Nhlh2 cDNA ORF clone, Mus musculus(house mouse)

Gene
Clones
gRNAs

Summary

Gene Symbol	Nhlh2
Entrez Gene ID	18072
Full Name	nescient helix loop helix 2
Synonyms	6230401I09Rik,Hen2,NSCL2,Nscl-2,bHLHa34
General protein information	Preferred Names nescient helix loop helix 2 Names helix-loop-helix protein 2 HEN-2
Gene Type	protein-coding
Organism	Mus musculus(house mouse)
Genome	Chromosome: 3 Map Location: 3\|3 F2.2

mRNA and Protein(s)

mRNA	Protein	Name
NM_178777.3	NP_848892.1	helix-loop-helix protein 2
XM_006501112.2	XP_006501175.1	helix-loop-helix protein 2 isoform X1
XM_006501112.3	XP_006501175.1	helix-loop-helix protein 2 isoform X1

Pathways from BioSystems

Homologies

Rattus norvegicus (Norway rat)	Nhlh2	NP_001099927.1
Homo sapiens (human)	NHLH2	NP_001104531.1
Canis lupus familiaris (dog)	NHLH2	XP_005630709.1
Mus musculus (house mouse)	Nhlh2	NP_848892.1
Pan troglodytes (chimpanzee)	NHLH2	XP_001148497.1
Gallus gallus (chicken)	NHLH2	NP_990128.1
Danio rerio (zebrafish)	nhlh2	NP_991232.1
	LOC709920	XP_001112098.1
Bos taurus (cattle)	NHLH2	NP_001103473.1

Gene Ontology
Bibliography

Related articles in PubMed

High-throughput discovery of novel developmental phenotypes.
Dickinson ME, Flenniken AM, Ji X, Teboul L, Wong MD, White JK, Meehan TF, Weninger WJ, Westerberg H, Adissu H, Baker CN, Bower L, Brown JM, Caddle LB, Chiani F, Clary D, Cleak J, Daly MJ, Denegre JM, Doe B, Dolan ME, Edie SM, Fuchs H, Gailus-Durner V, Galli A, Gambadoro A, Gallegos J, Guo S, Horner NR, Hsu CW, Johnson SJ, Kalaga S, Keith LC, Lanoue L, Lawson TN, Lek M, Mark M, Marschall S, Mason J, McElwee ML, Newbigging S, Nutter LM, Peterson KA, Ramirez-Solis R, Rowland DJ, Ryder E, Samocha KE, Seavitt JR, Selloum M, Szoke-Kovacs Z, Tamura M, Trainor AG, Tudose I, Wakana S, Warren J, Wendling O, West DB, Wong L, Yoshiki A, , , , , , , , , MacArthur DG, Tocchini-Valentini GP, Gao X, Flicek P, Bradley A, Skarnes WC, Justice MJ, Parkinson HE, Moore M, Wells S, Braun RE, Svenson KL, de Angelis MH, Herault Y, Mohun T, Mallon AM, Henkelman RM, Brown SD, Adams DJ, Lloyd KC, McKerlie C, Beaudet AL, Bu?an M, Murray SA
Nature537(7621)508-514(2016 09)

Polo-Like Kinase 3 Appears Dispensable for Normal Retinal Development Despite Robust Embryonic Expression.
Goetz JJ, Laboissonniere LA, Wester AK, Lynch MR, Trimarchi JM
PloS one11(3)e0150878(2016)

NeuroD1 reprograms chromatin and transcription factor landscapes to induce the neuronal program.
Pataskar A, Jung J, Smialowski P, Noack F, Calegari F, Straub T, Tiwari VK
The EMBO journal35(1)24-45(2016 Jan)

Oncogenic Lmo3 cooperates with Hen2 to induce hydrocephalus in mice.
Isogai E, Okumura K, Saito M, Yoshizawa Y, Itoh K, Tando S, Ohira M, Haraguchi S, Nakagawara A, Fushiki S, Nagase H, Wakabayashi Y
Experimental animals64(4)407-14(2015)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

suggested that the expression levels of Lmo3 and/or Hen2 could determine the fate of stem cells by inhibiting Hes1 function during nervous system development and might be a trigger of aberrant neurogenesis in vivo
Title: Oncogenic Lmo3 cooperates with Hen2 to induce hydrocephalus in mice.

We found that loss of Nscl-2 results in aberrant migration of gonadotropin releasing hormone neurons in Nscl-2 mutant mice causing a lineage switch of ectopically located gonadotropin releasing hormone neurons
Title: Loss of NSCL-2 in gonadotropin releasing hormone neurons leads to reduction of pro-opiomelanocortin neurons in specific hypothalamic nuclei and causes visceral obesity.

Melanocortin 4 receptor is a transcriptional target of nescient helix-loop-helix-2
Title: Melanocortin 4 receptor is a transcriptional target of nescient helix-loop-helix-2.

Nhlh2 in the central control of adipocyte function
Title: Deletion of Nhlh2 results in a defective torpor response and reduced Beta adrenergic receptor expression in adipose tissue.

the reduction of the innervation and vascularization of white adipose tissue in Nscl-2 mutant mice leads to the increase of preadipocyte/macrophage-like cells, a bimodal distribution of the size of adipocytes
Title: Defective peripheral nerve development is linked to abnormal architecture and metabolic activity of adipose tissue in Nscl-2 mutant mice.

The following Nhlh2 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Nhlh2 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OMu17659	NM_178777.3 Latest version!	Mus musculus nescient helix loop helix 2 (Nhlh2), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$69.30 $99.00
OMu17659	XM_006501112.3 Latest version!	Mus musculus nescient helix loop helix 2 (Nhlh2), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$69.30 $99.00
View Old Accession Versions >>
OMu17659	XM_006501112.2	Mus musculus nescient helix loop helix 2 (Nhlh2), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$69.30 $99.00
Hide Old Accession Versions >>

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Nhlh2 ( NM_178777.3 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_848892.1 Mus musculus nescient helix loop helix 2 (Nhlh2), mRNA.

Price & Availability↑

CloneID	OMu17659
Clone ID Related Accession (Same CDS sequence)	NM_178777.3 , XM_006501112.2 , XM_006501112.3
Accession Version	NM_178777.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 408bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-27
Organism	Mus musculus(house mouse)
Product	helix-loop-helix protein 2
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AK031747.1 and AC166072.2. On Jul 16, 2009 this sequence version replaced NM_178777.2. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AK084308.1, BC058413.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00849383, SAMN01164131 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGATGCTGA GTCCGGACCA AGCCGCCGAC TCAGATCACC CCAGCTCGAC GCACTCGGAC 
CCGGAGTCTC TGGGCGGCGC AGACACCAAG GTTCTGGGCA GCGTGTCGGA CCTAGAGCCA 
GTGGAGGAGG CGGACGGCGA CGGCAAGGGA GGCAGCCGGG CCGCGCTCTA CCCGCACCCG 
CAGCAGCTGA GCCGCGAAGA AAAGCGCCGC CGCCGGCGCG CCACGGCCAA GTACCGATCG 
GCCCACGCCA CCCGCGAGCG CATCCGAGTG GAGGCTTTCA ACCTGGCCTT CGCCGAGCTC 
CGCAAACTAC TACCCACGCT GCCTCCGGAC AAGAAGCTCT CCAAGATCGA GATTCTGCGC 
TTGGCCATCT GCTACATCTC CTATCTCAAC CATGTCCTGG ACGTGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_848892.1
CDS	515..922
Translation	MMLSPDQAADSDHPSSTHSDPESLGGADTKVLGSVSDLEPVEEADGDGKGGSRAALYPHPQQLSREEKRRRRRATAKYRSAHATRERIRVEAFNLAFAELRKLLPTLPPDKKLSKIEILRLAICYISYLNHVLDV

Target ORF information:

RefSeq Version	NM_178777.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus nescient helix loop helix 2 (Nhlh2), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_178777.3

ORF Insert Sequence:

ATGATGCTGA GTCCGGACCA AGCCGCCGAC TCAGATCACC CCAGCTCGAC GCACTCGGAC 
CCGGAGTCTC TGGGCGGCGC AGACACCAAG GTTCTGGGCA GCGTGTCGGA CCTAGAGCCA 
GTGGAGGAGG CGGACGGCGA CGGCAAGGGA GGCAGCCGGG CCGCGCTCTA CCCGCACCCG 
CAGCAGCTGA GCCGCGAAGA AAAGCGCCGC CGCCGGCGCG CCACGGCCAA GTACCGATCG 
GCCCACGCCA CCCGCGAGCG CATCCGAGTG GAGGCTTTCA ACCTGGCCTT CGCCGAGCTC 
CGCAAACTAC TACCCACGCT GCCTCCGGAC AAGAAGCTCT CCAAGATCGA GATTCTGCGC 
TTGGCCATCT GCTACATCTC CTATCTCAAC CATGTCCTGG ACGTGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Nhlh2 ( XM_006501112.2 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006501175.1 Mus musculus nescient helix loop helix 2 (Nhlh2), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OMu17659
Clone ID Related Accession (Same CDS sequence)	NM_178777.3 , XM_006501112.2 , XM_006501112.3
Accession Version	XM_006501112.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 408bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	helix-loop-helix protein 2 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000069.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Feb 9, 2015 this sequence version replaced XM_006501112.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGATGCTGA GTCCGGACCA AGCCGCCGAC TCAGATCACC CCAGCTCGAC GCACTCGGAC 
CCGGAGTCTC TGGGCGGCGC AGACACCAAG GTTCTGGGCA GCGTGTCGGA CCTAGAGCCA 
GTGGAGGAGG CGGACGGCGA CGGCAAGGGA GGCAGCCGGG CCGCGCTCTA CCCGCACCCG 
CAGCAGCTGA GCCGCGAAGA AAAGCGCCGC CGCCGGCGCG CCACGGCCAA GTACCGATCG 
GCCCACGCCA CCCGCGAGCG CATCCGAGTG GAGGCTTTCA ACCTGGCCTT CGCCGAGCTC 
CGCAAACTAC TACCCACGCT GCCTCCGGAC AAGAAGCTCT CCAAGATCGA GATTCTGCGC 
TTGGCCATCT GCTACATCTC CTATCTCAAC CATGTCCTGG ACGTGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006501175.1
CDS	300..707
Translation	MMLSPDQAADSDHPSSTHSDPESLGGADTKVLGSVSDLEPVEEADGDGKGGSRAALYPHPQQLSREEKRRRRRATAKYRSAHATRERIRVEAFNLAFAELRKLLPTLPPDKKLSKIEILRLAICYISYLNHVLDV

Target ORF information:

RefSeq Version	XM_006501112.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus nescient helix loop helix 2 (Nhlh2), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006501112.2

ORF Insert Sequence:

ATGATGCTGA GTCCGGACCA AGCCGCCGAC TCAGATCACC CCAGCTCGAC GCACTCGGAC 
CCGGAGTCTC TGGGCGGCGC AGACACCAAG GTTCTGGGCA GCGTGTCGGA CCTAGAGCCA 
GTGGAGGAGG CGGACGGCGA CGGCAAGGGA GGCAGCCGGG CCGCGCTCTA CCCGCACCCG 
CAGCAGCTGA GCCGCGAAGA AAAGCGCCGC CGCCGGCGCG CCACGGCCAA GTACCGATCG 
GCCCACGCCA CCCGCGAGCG CATCCGAGTG GAGGCTTTCA ACCTGGCCTT CGCCGAGCTC 
CGCAAACTAC TACCCACGCT GCCTCCGGAC AAGAAGCTCT CCAAGATCGA GATTCTGCGC 
TTGGCCATCT GCTACATCTC CTATCTCAAC CATGTCCTGG ACGTGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Nhlh2 ( XM_006501112.3 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006501175.1 Mus musculus nescient helix loop helix 2 (Nhlh2), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OMu17659
Clone ID Related Accession (Same CDS sequence)	NM_178777.3 , XM_006501112.2 , XM_006501112.3
Accession Version	XM_006501112.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 408bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	helix-loop-helix protein 2 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000069.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_006501112.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGATGCTGA GTCCGGACCA AGCCGCCGAC TCAGATCACC CCAGCTCGAC GCACTCGGAC 
CCGGAGTCTC TGGGCGGCGC AGACACCAAG GTTCTGGGCA GCGTGTCGGA CCTAGAGCCA 
GTGGAGGAGG CGGACGGCGA CGGCAAGGGA GGCAGCCGGG CCGCGCTCTA CCCGCACCCG 
CAGCAGCTGA GCCGCGAAGA AAAGCGCCGC CGCCGGCGCG CCACGGCCAA GTACCGATCG 
GCCCACGCCA CCCGCGAGCG CATCCGAGTG GAGGCTTTCA ACCTGGCCTT CGCCGAGCTC 
CGCAAACTAC TACCCACGCT GCCTCCGGAC AAGAAGCTCT CCAAGATCGA GATTCTGCGC 
TTGGCCATCT GCTACATCTC CTATCTCAAC CATGTCCTGG ACGTGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006501175.1
CDS	299..706
Translation	MMLSPDQAADSDHPSSTHSDPESLGGADTKVLGSVSDLEPVEEADGDGKGGSRAALYPHPQQLSREEKRRRRRATAKYRSAHATRERIRVEAFNLAFAELRKLLPTLPPDKKLSKIEILRLAICYISYLNHVLDV

Target ORF information:

RefSeq Version	XM_006501112.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus nescient helix loop helix 2 (Nhlh2), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006501112.3

ORF Insert Sequence:

ATGATGCTGA GTCCGGACCA AGCCGCCGAC TCAGATCACC CCAGCTCGAC GCACTCGGAC 
CCGGAGTCTC TGGGCGGCGC AGACACCAAG GTTCTGGGCA GCGTGTCGGA CCTAGAGCCA 
GTGGAGGAGG CGGACGGCGA CGGCAAGGGA GGCAGCCGGG CCGCGCTCTA CCCGCACCCG 
CAGCAGCTGA GCCGCGAAGA AAAGCGCCGC CGCCGGCGCG CCACGGCCAA GTACCGATCG 
GCCCACGCCA CCCGCGAGCG CATCCGAGTG GAGGCTTTCA ACCTGGCCTT CGCCGAGCTC 
CGCAAACTAC TACCCACGCT GCCTCCGGAC AAGAAGCTCT CCAAGATCGA GATTCTGCGC 
TTGGCCATCT GCTACATCTC CTATCTCAAC CATGTCCTGG ACGTGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

High-throughput discovery of novel developmental phenotypes.
Nature537(7621)508-514(2016 09)
Dickinson ME,Flenniken AM,Ji X,Teboul L,Wong MD,White JK,Meehan TF,Weninger WJ,Westerberg H,Adissu H,Baker CN,Bower L,Brown JM,Caddle LB,Chiani F,Clary D,Cleak J,Daly MJ,Denegre JM,Doe B,Dolan ME,Edie SM,Fuchs H,Gailus-Durner V,Galli A,Gambadoro A,Gallegos J,Guo S,Horner NR,Hsu CW,Johnson SJ,Kalaga S,Keith LC,Lanoue L,Lawson TN,Lek M,Mark M,Marschall S,Mason J,McElwee ML,Newbigging S,Nutter LM,Peterson KA,Ramirez-Solis R,Rowland DJ,Ryder E,Samocha KE,Seavitt JR,Selloum M,Szoke-Kovacs Z,Tamura M,Trainor AG,Tudose I,Wakana S,Warren J,Wendling O,West DB,Wong L,Yoshiki A,,,,,,,,,MacArthur DG,Tocchini-Valentini GP,Gao X,Flicek P,Bradley A,Skarnes WC,Justice MJ,Parkinson HE,Moore M,Wells S,Braun RE,Svenson KL,de Angelis MH,Herault Y,Mohun T,Mallon AM,Henkelman RM,Brown SD,Adams DJ,Lloyd KC,McKerlie C,Beaudet AL,Bu?an M,Murray SA

Polo-Like Kinase 3 Appears Dispensable for Normal Retinal Development Despite Robust Embryonic Expression.
PloS one11(3)e0150878(2016)
Goetz JJ,Laboissonniere LA,Wester AK,Lynch MR,Trimarchi JM

NeuroD1 reprograms chromatin and transcription factor landscapes to induce the neuronal program.
The EMBO journal35(1)24-45(2016 Jan)
Pataskar A,Jung J,Smialowski P,Noack F,Calegari F,Straub T,Tiwari VK

Oncogenic Lmo3 cooperates with Hen2 to induce hydrocephalus in mice.
Experimental animals64(4)407-14(2015)
Isogai E,Okumura K,Saito M,Yoshizawa Y,Itoh K,Tando S,Ohira M,Haraguchi S,Nakagawara A,Fushiki S,Nagase H,Wakabayashi Y

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Nhlh2 cDNA ORF clone, Mus musculus(house mouse)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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