LOC111021883 cDNA ORF clone, Momordica charantia(bitter melon)

Gene
Clones
gRNAs

Summary

Gene Symbol	LOC111021883
Entrez Gene ID	111021883
Full Name	putative disease resistance protein RGA3
Gene Type	protein-coding
Organism	Momordica charantia(bitter melon)

mRNA and Protein(s)

mRNA	Protein	Name
XM_022299005.1	XP_022154697.1	putative disease resistance protein RGA3
XM_022298997.1	XP_022154689.1	putative disease resistance protein RGA3

Pathways from BioSystems
Gene Ontology

The following LOC111021883 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the LOC111021883 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OMo120674	XM_022298997.1 Latest version!	Momordica charantia putative disease resistance protein RGA3 (LOC111021883), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$510.30 $729.00
OMo120674	XM_022299005.1 Latest version!	Momordica charantia putative disease resistance protein RGA3 (LOC111021883), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$510.30 $729.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
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2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
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*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

LOC111021883 ( XM_022299005.1 ) cDNA ORF clone, Momordica charantia(bitter melon) -> XP_022154697.1 Momordica charantia putative disease resistance protein RGA3 (LOC111021883), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OMo120674
Clone ID Related Accession (Same CDS sequence)	XM_022299005.1 , XM_022298997.1
Accession Version	XM_022299005.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2634bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-08-10
Organism	Momordica charantia(bitter melon)
Product	putative disease resistance protein RGA3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_019104489.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Momordica charantia Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCGCAAT CAATTTTGTT CAATGTTGCG GGCAGCCTTA TAATGAAACT GGGCTCTCTT 
GCATTCCGAG AGCTTGCTTT ACTCTGGGGT GTTGATGATG AGCTTGAAAA ACTCAAAGAC 
GCTGTTTCTG CCATTAAAGC CGTACTTTTG GATGCAGAGG AGCAGCAGTA CAAGAGCCAC 
GCGGTGAAGA ATTGGGTTTC GAGGCTTAAA GATGTTCTCT ACGATGTTGA CGACTTGATT 
GATGAGTTCT CTTATGAAAC CTTGAGAAGA CAAGTTAAGG CCAAAGATGG ACGAAAGCCT 
GAACAGGTAT GTATGTTCTT CTCCAAGTCA AATCCAGTTG CATTTGGTTT TAGAATGGGT 
CAAACAATAA AGGACATTAG GGAGAAATTA AATGGCATTA ATGCTGATAA AACCCAATTT 
CACTTTTCTG AGCGTACGAT GGACACGCCA GATGATGGGC TTAGGAAGGG ACGAGAGACT 
TACTCTTTTA TACCTGAAGG TGAAGTAATT GGTAGGGATG ATGACAGAAA AGCAATTGCA 
GATCTTTTGT TAGATACCAA CTTCAAAGAG GATATTGCAG TGATTGCTAT AGTTGGAATG 
GGAGGATTAG GAAAGACCAC ACTTGCTCAA TCTGTCTATA ACGATGAGAT GATAAGAAAA 
CATTTTGCAC TAAAATTGTG GGTGTGTGTT TCTGAAGAAT TTGATGTTAA AATTATTGTA 
GAAAAGATTC TGGAGTCTGC AACAATGAAG AGGCACGGGC CTTTTGAAAT AGATACATTG 
CAAAGTTTAC TTCGAAAGGA AATTGATGGA AAGAAATATC TATTCGTCAT GGATGATGTG 
TGGAATGAGA ACTATGAGAA ATGGGTCCAT TTGAAAAGAT TATTAATGTG TGGAGCAACT 
GGAAGTAGGA TTTTGATCAC CACGCGTAGT ATACAAGTTG CAAAAACATT TGATGTAGTA 
TCACTATATC CTTTAGAAAA TCTAAATGAA GACGACTCGT GGTTATTGTT TAAAAAGATG 
GCATCTTTGA AAGGAGCAGA AGAAGAGCTT GGAAATTCAA ATCTTTTCAA AATTGGCAAA 
GAGATTGTTG CAAAGTTGAA AGGTGTTCCT CTTGCAATAA GAACTATTTG GAGACTTCTA 
TGTTCCAAAA AATCAGAAGC TGACTGGTTA TTTTTCAAAA ATAATGAACT TTCAAGAGCG 
GTACGACAAG AAAATGAGAT GCAATCAATA CTTAAGATTA GTTATAACCA CCTTTCGTCT 
AATTTAAAGC AATGTTTTGC ATATTGTGCT TTATTCCCTA AAAATTATGA GATTCGAAAG 
GATGAGGTGA TAAAGCAATG GGTAGCACAA GGTTTTATTC AACCACAGAG TATTAAAGCA 
ATTGAAGATG TTGGTGAAGA TTATTTTATG GAGTTATTGT GGAGGTCGTT CTTTCAAGAT 
ATAAGGAGTA ATGAATTGGG AGACATCAAA CAGTTTAAGA TGCACGACTT AATGCATGAT 
CTTGCATGTT CGATAGATGA AAATGAATGC GTGCTTGCTA GTTTGAATGC TAGGTCTATC 
GTAAAAAGAA CTCGACACGT GTTATTTGAG TTTAAGTTAA ATGAAACGTG GGAACTTCCA 
GAATCATTAA TTGAGGCAAA GAATTTGACA ACATTGTATA ATCCTCCATT TGATCGTAAG 
AAACCCCTCC ACAAGATGTT TTCTAATCAT TTGCGATTAC GAACATTGGT TCTGGTGTAT 
AGCTTTATTA TAGAATTGCC CAAGTCCACT AGCAAGTTGA AACATTTGCG ATATCTTGAC 
CTTAGTTGTA ATAATTATAT AGAATTCCTT CCAAATTCTA TCACTGAATT GTATAATTTG 
GAAACACTTC TCCTTTGTGG TTGCACAGGT TCGAGAACAT TGCCAAGAGA TACTAAAAAT 
TTGATCAGCC TTAGACATCT TGATATGTGT GATTGTTATG CATTGACTCA TATGCCAAAA 
GGATTGGGTG AGATGACTAG TCTTCAGACA ATGAATTTAT TTGTATTAGG AGATACTAAT 
AGCAACGGAT TAAGTGAGTT GAATGGACTT GGAAATTTGA AGGGATCGTT AACTATTCGA 
GATTTACAAT TTCAGACTAT TGGTGATTTA CAAAAAAATG CTTATTACTT GAAATTAAAG 
TCAGGAATTA AACGGTTGAT GTTACATTGG AAGGATGGTC GTGGAAAGAA GTCTAAAATT 
GTTGCCTTGG ATGATGAAGA AAGAGTTTTA GAATGCTTAG AACCACATCC AAATCTTCAA 
AAGATAGAAA TATTGGGATA CCATGGAGCA AGGCTTTGTA ATTGGCTATC CTCTGATTCG 
CTTGTTCGTC TGGTCACCAT AAAACTTTCA CATTGTAGTA GATTGCAGCA GCTCCCTCAA 
TTTGATCAAT ATCCTTTTCT CAAGCATCTT TGTCTTGAAT CCTTGATCTG TATCGAGTAC 
ATTGACAACA ATAGTAATAT TTACCTTTCT TCATCAACGT TCTTCCCATC CCTTGAGAAA 
TTAGAAATTT CAACCATGCC AAACTTGAAG GGATGGTGGA AAGGCGAAAC GTCAGGGGAA 
TTAGCTCAAA ATAGTGTCTC ATTTCCAACA ATGATGCCTT GCCTTTCTAC GTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_022154697.1
CDS	27..2660
Translation	MAQSILFNVAGSLIMKLGSLAFRELALLWGVDDELEKLKDAVSAIKAVLLDAEEQQYKSHAVKNWVSRLKDVLYDVDDLIDEFSYETLRRQVKAKDGRKPEQVCMFFSKSNPVAFGFRMGQTIKDIREKLNGINADKTQFHFSERTMDTPDDGLRKGRETYSFIPEGEVIGRDDDRKAIADLLLDTNFKEDIAVIAIVGMGGLGKTTLAQSVYNDEMIRKHFALKLWVCVSEEFDVKIIVEKILESATMKRHGPFEIDTLQSLLRKEIDGKKYLFVMDDVWNENYEKWVHLKRLLMCGATGSRILITTRSIQVAKTFDVVSLYPLENLNEDDSWLLFKKMASLKGAEEELGNSNLFKIGKEIVAKLKGVPLAIRTIWRLLCSKKSEADWLFFKNNELSRAVRQENEMQSILKISYNHLSSNLKQCFAYCALFPKNYEIRKDEVIKQWVAQGFIQPQSIKAIEDVGEDYFMELLWRSFFQDIRSNELGDIKQFKMHDLMHDLACSIDENECVLASLNARSIVKRTRHVLFEFKLNETWELPESLIEAKNLTTLYNPPFDRKKPLHKMFSNHLRLRTLVLVYSFIIELPKSTSKLKHLRYLDLSCNNYIEFLPNSITELYNLETLLLCGCTGSRTLPRDTKNLISLRHLDMCDCYALTHMPKGLGEMTSLQTMNLFVLGDTNSNGLSELNGLGNLKGSLTIRDLQFQTIGDLQKNAYYLKLKSGIKRLMLHWKDGRGKKSKIVALDDEERVLECLEPHPNLQKIEILGYHGARLCNWLSSDSLVRLVTIKLSHCSRLQQLPQFDQYPFLKHLCLESLICIEYIDNNSNIYLSSSTFFPSLEKLEISTMPNLKGWWKGETSGELAQNSVSFPTMMPCLST

Target ORF information:

RefSeq Version	XM_022299005.1
Organism	Momordica charantia(bitter melon)
Definition	Momordica charantia putative disease resistance protein RGA3 (LOC111021883), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_022299005.1

ORF Insert Sequence:

ATGGCGCAAT CAATTTTGTT CAATGTTGCG GGCAGCCTTA TAATGAAACT GGGCTCTCTT 
GCATTCCGAG AGCTTGCTTT ACTCTGGGGT GTTGATGATG AGCTTGAAAA ACTCAAAGAC 
GCTGTTTCTG CCATTAAAGC CGTACTTTTG GATGCAGAGG AGCAGCAGTA CAAGAGCCAC 
GCGGTGAAGA ATTGGGTTTC GAGGCTTAAA GATGTTCTCT ACGATGTTGA CGACTTGATT 
GATGAGTTCT CTTATGAAAC CTTGAGAAGA CAAGTTAAGG CCAAAGATGG ACGAAAGCCT 
GAACAGGTAT GTATGTTCTT CTCCAAGTCA AATCCAGTTG CATTTGGTTT TAGAATGGGT 
CAAACAATAA AGGACATTAG GGAGAAATTA AATGGCATTA ATGCTGATAA AACCCAATTT 
CACTTTTCTG AGCGTACGAT GGACACGCCA GATGATGGGC TTAGGAAGGG ACGAGAGACT 
TACTCTTTTA TACCTGAAGG TGAAGTAATT GGTAGGGATG ATGACAGAAA AGCAATTGCA 
GATCTTTTGT TAGATACCAA CTTCAAAGAG GATATTGCAG TGATTGCTAT AGTTGGAATG 
GGAGGATTAG GAAAGACCAC ACTTGCTCAA TCTGTCTATA ACGATGAGAT GATAAGAAAA 
CATTTTGCAC TAAAATTGTG GGTGTGTGTT TCTGAAGAAT TTGATGTTAA AATTATTGTA 
GAAAAGATTC TGGAGTCTGC AACAATGAAG AGGCACGGGC CTTTTGAAAT AGATACATTG 
CAAAGTTTAC TTCGAAAGGA AATTGATGGA AAGAAATATC TATTCGTCAT GGATGATGTG 
TGGAATGAGA ACTATGAGAA ATGGGTCCAT TTGAAAAGAT TATTAATGTG TGGAGCAACT 
GGAAGTAGGA TTTTGATCAC CACGCGTAGT ATACAAGTTG CAAAAACATT TGATGTAGTA 
TCACTATATC CTTTAGAAAA TCTAAATGAA GACGACTCGT GGTTATTGTT TAAAAAGATG 
GCATCTTTGA AAGGAGCAGA AGAAGAGCTT GGAAATTCAA ATCTTTTCAA AATTGGCAAA 
GAGATTGTTG CAAAGTTGAA AGGTGTTCCT CTTGCAATAA GAACTATTTG GAGACTTCTA 
TGTTCCAAAA AATCAGAAGC TGACTGGTTA TTTTTCAAAA ATAATGAACT TTCAAGAGCG 
GTACGACAAG AAAATGAGAT GCAATCAATA CTTAAGATTA GTTATAACCA CCTTTCGTCT 
AATTTAAAGC AATGTTTTGC ATATTGTGCT TTATTCCCTA AAAATTATGA GATTCGAAAG 
GATGAGGTGA TAAAGCAATG GGTAGCACAA GGTTTTATTC AACCACAGAG TATTAAAGCA 
ATTGAAGATG TTGGTGAAGA TTATTTTATG GAGTTATTGT GGAGGTCGTT CTTTCAAGAT 
ATAAGGAGTA ATGAATTGGG AGACATCAAA CAGTTTAAGA TGCACGACTT AATGCATGAT 
CTTGCATGTT CGATAGATGA AAATGAATGC GTGCTTGCTA GTTTGAATGC TAGGTCTATC 
GTAAAAAGAA CTCGACACGT GTTATTTGAG TTTAAGTTAA ATGAAACGTG GGAACTTCCA 
GAATCATTAA TTGAGGCAAA GAATTTGACA ACATTGTATA ATCCTCCATT TGATCGTAAG 
AAACCCCTCC ACAAGATGTT TTCTAATCAT TTGCGATTAC GAACATTGGT TCTGGTGTAT 
AGCTTTATTA TAGAATTGCC CAAGTCCACT AGCAAGTTGA AACATTTGCG ATATCTTGAC 
CTTAGTTGTA ATAATTATAT AGAATTCCTT CCAAATTCTA TCACTGAATT GTATAATTTG 
GAAACACTTC TCCTTTGTGG TTGCACAGGT TCGAGAACAT TGCCAAGAGA TACTAAAAAT 
TTGATCAGCC TTAGACATCT TGATATGTGT GATTGTTATG CATTGACTCA TATGCCAAAA 
GGATTGGGTG AGATGACTAG TCTTCAGACA ATGAATTTAT TTGTATTAGG AGATACTAAT 
AGCAACGGAT TAAGTGAGTT GAATGGACTT GGAAATTTGA AGGGATCGTT AACTATTCGA 
GATTTACAAT TTCAGACTAT TGGTGATTTA CAAAAAAATG CTTATTACTT GAAATTAAAG 
TCAGGAATTA AACGGTTGAT GTTACATTGG AAGGATGGTC GTGGAAAGAA GTCTAAAATT 
GTTGCCTTGG ATGATGAAGA AAGAGTTTTA GAATGCTTAG AACCACATCC AAATCTTCAA 
AAGATAGAAA TATTGGGATA CCATGGAGCA AGGCTTTGTA ATTGGCTATC CTCTGATTCG 
CTTGTTCGTC TGGTCACCAT AAAACTTTCA CATTGTAGTA GATTGCAGCA GCTCCCTCAA 
TTTGATCAAT ATCCTTTTCT CAAGCATCTT TGTCTTGAAT CCTTGATCTG TATCGAGTAC 
ATTGACAACA ATAGTAATAT TTACCTTTCT TCATCAACGT TCTTCCCATC CCTTGAGAAA 
TTAGAAATTT CAACCATGCC AAACTTGAAG GGATGGTGGA AAGGCGAAAC GTCAGGGGAA 
TTAGCTCAAA ATAGTGTCTC ATTTCCAACA ATGATGCCTT GCCTTTCTAC GTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC111021883 ( XM_022298997.1 ) cDNA ORF clone, Momordica charantia(bitter melon) -> XP_022154689.1 Momordica charantia putative disease resistance protein RGA3 (LOC111021883), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OMo120674
Clone ID Related Accession (Same CDS sequence)	XM_022299005.1 , XM_022298997.1
Accession Version	XM_022298997.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2634bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-08-10
Organism	Momordica charantia(bitter melon)
Product	putative disease resistance protein RGA3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_019104489.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Momordica charantia Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCGCAAT CAATTTTGTT CAATGTTGCG GGCAGCCTTA TAATGAAACT GGGCTCTCTT 
GCATTCCGAG AGCTTGCTTT ACTCTGGGGT GTTGATGATG AGCTTGAAAA ACTCAAAGAC 
GCTGTTTCTG CCATTAAAGC CGTACTTTTG GATGCAGAGG AGCAGCAGTA CAAGAGCCAC 
GCGGTGAAGA ATTGGGTTTC GAGGCTTAAA GATGTTCTCT ACGATGTTGA CGACTTGATT 
GATGAGTTCT CTTATGAAAC CTTGAGAAGA CAAGTTAAGG CCAAAGATGG ACGAAAGCCT 
GAACAGGTAT GTATGTTCTT CTCCAAGTCA AATCCAGTTG CATTTGGTTT TAGAATGGGT 
CAAACAATAA AGGACATTAG GGAGAAATTA AATGGCATTA ATGCTGATAA AACCCAATTT 
CACTTTTCTG AGCGTACGAT GGACACGCCA GATGATGGGC TTAGGAAGGG ACGAGAGACT 
TACTCTTTTA TACCTGAAGG TGAAGTAATT GGTAGGGATG ATGACAGAAA AGCAATTGCA 
GATCTTTTGT TAGATACCAA CTTCAAAGAG GATATTGCAG TGATTGCTAT AGTTGGAATG 
GGAGGATTAG GAAAGACCAC ACTTGCTCAA TCTGTCTATA ACGATGAGAT GATAAGAAAA 
CATTTTGCAC TAAAATTGTG GGTGTGTGTT TCTGAAGAAT TTGATGTTAA AATTATTGTA 
GAAAAGATTC TGGAGTCTGC AACAATGAAG AGGCACGGGC CTTTTGAAAT AGATACATTG 
CAAAGTTTAC TTCGAAAGGA AATTGATGGA AAGAAATATC TATTCGTCAT GGATGATGTG 
TGGAATGAGA ACTATGAGAA ATGGGTCCAT TTGAAAAGAT TATTAATGTG TGGAGCAACT 
GGAAGTAGGA TTTTGATCAC CACGCGTAGT ATACAAGTTG CAAAAACATT TGATGTAGTA 
TCACTATATC CTTTAGAAAA TCTAAATGAA GACGACTCGT GGTTATTGTT TAAAAAGATG 
GCATCTTTGA AAGGAGCAGA AGAAGAGCTT GGAAATTCAA ATCTTTTCAA AATTGGCAAA 
GAGATTGTTG CAAAGTTGAA AGGTGTTCCT CTTGCAATAA GAACTATTTG GAGACTTCTA 
TGTTCCAAAA AATCAGAAGC TGACTGGTTA TTTTTCAAAA ATAATGAACT TTCAAGAGCG 
GTACGACAAG AAAATGAGAT GCAATCAATA CTTAAGATTA GTTATAACCA CCTTTCGTCT 
AATTTAAAGC AATGTTTTGC ATATTGTGCT TTATTCCCTA AAAATTATGA GATTCGAAAG 
GATGAGGTGA TAAAGCAATG GGTAGCACAA GGTTTTATTC AACCACAGAG TATTAAAGCA 
ATTGAAGATG TTGGTGAAGA TTATTTTATG GAGTTATTGT GGAGGTCGTT CTTTCAAGAT 
ATAAGGAGTA ATGAATTGGG AGACATCAAA CAGTTTAAGA TGCACGACTT AATGCATGAT 
CTTGCATGTT CGATAGATGA AAATGAATGC GTGCTTGCTA GTTTGAATGC TAGGTCTATC 
GTAAAAAGAA CTCGACACGT GTTATTTGAG TTTAAGTTAA ATGAAACGTG GGAACTTCCA 
GAATCATTAA TTGAGGCAAA GAATTTGACA ACATTGTATA ATCCTCCATT TGATCGTAAG 
AAACCCCTCC ACAAGATGTT TTCTAATCAT TTGCGATTAC GAACATTGGT TCTGGTGTAT 
AGCTTTATTA TAGAATTGCC CAAGTCCACT AGCAAGTTGA AACATTTGCG ATATCTTGAC 
CTTAGTTGTA ATAATTATAT AGAATTCCTT CCAAATTCTA TCACTGAATT GTATAATTTG 
GAAACACTTC TCCTTTGTGG TTGCACAGGT TCGAGAACAT TGCCAAGAGA TACTAAAAAT 
TTGATCAGCC TTAGACATCT TGATATGTGT GATTGTTATG CATTGACTCA TATGCCAAAA 
GGATTGGGTG AGATGACTAG TCTTCAGACA ATGAATTTAT TTGTATTAGG AGATACTAAT 
AGCAACGGAT TAAGTGAGTT GAATGGACTT GGAAATTTGA AGGGATCGTT AACTATTCGA 
GATTTACAAT TTCAGACTAT TGGTGATTTA CAAAAAAATG CTTATTACTT GAAATTAAAG 
TCAGGAATTA AACGGTTGAT GTTACATTGG AAGGATGGTC GTGGAAAGAA GTCTAAAATT 
GTTGCCTTGG ATGATGAAGA AAGAGTTTTA GAATGCTTAG AACCACATCC AAATCTTCAA 
AAGATAGAAA TATTGGGATA CCATGGAGCA AGGCTTTGTA ATTGGCTATC CTCTGATTCG 
CTTGTTCGTC TGGTCACCAT AAAACTTTCA CATTGTAGTA GATTGCAGCA GCTCCCTCAA 
TTTGATCAAT ATCCTTTTCT CAAGCATCTT TGTCTTGAAT CCTTGATCTG TATCGAGTAC 
ATTGACAACA ATAGTAATAT TTACCTTTCT TCATCAACGT TCTTCCCATC CCTTGAGAAA 
TTAGAAATTT CAACCATGCC AAACTTGAAG GGATGGTGGA AAGGCGAAAC GTCAGGGGAA 
TTAGCTCAAA ATAGTGTCTC ATTTCCAACA ATGATGCCTT GCCTTTCTAC GTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_022154689.1
CDS	27..2660
Translation	MAQSILFNVAGSLIMKLGSLAFRELALLWGVDDELEKLKDAVSAIKAVLLDAEEQQYKSHAVKNWVSRLKDVLYDVDDLIDEFSYETLRRQVKAKDGRKPEQVCMFFSKSNPVAFGFRMGQTIKDIREKLNGINADKTQFHFSERTMDTPDDGLRKGRETYSFIPEGEVIGRDDDRKAIADLLLDTNFKEDIAVIAIVGMGGLGKTTLAQSVYNDEMIRKHFALKLWVCVSEEFDVKIIVEKILESATMKRHGPFEIDTLQSLLRKEIDGKKYLFVMDDVWNENYEKWVHLKRLLMCGATGSRILITTRSIQVAKTFDVVSLYPLENLNEDDSWLLFKKMASLKGAEEELGNSNLFKIGKEIVAKLKGVPLAIRTIWRLLCSKKSEADWLFFKNNELSRAVRQENEMQSILKISYNHLSSNLKQCFAYCALFPKNYEIRKDEVIKQWVAQGFIQPQSIKAIEDVGEDYFMELLWRSFFQDIRSNELGDIKQFKMHDLMHDLACSIDENECVLASLNARSIVKRTRHVLFEFKLNETWELPESLIEAKNLTTLYNPPFDRKKPLHKMFSNHLRLRTLVLVYSFIIELPKSTSKLKHLRYLDLSCNNYIEFLPNSITELYNLETLLLCGCTGSRTLPRDTKNLISLRHLDMCDCYALTHMPKGLGEMTSLQTMNLFVLGDTNSNGLSELNGLGNLKGSLTIRDLQFQTIGDLQKNAYYLKLKSGIKRLMLHWKDGRGKKSKIVALDDEERVLECLEPHPNLQKIEILGYHGARLCNWLSSDSLVRLVTIKLSHCSRLQQLPQFDQYPFLKHLCLESLICIEYIDNNSNIYLSSSTFFPSLEKLEISTMPNLKGWWKGETSGELAQNSVSFPTMMPCLST

Target ORF information:

RefSeq Version	XM_022298997.1
Organism	Momordica charantia(bitter melon)
Definition	Momordica charantia putative disease resistance protein RGA3 (LOC111021883), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_022298997.1

ORF Insert Sequence:

ATGGCGCAAT CAATTTTGTT CAATGTTGCG GGCAGCCTTA TAATGAAACT GGGCTCTCTT 
GCATTCCGAG AGCTTGCTTT ACTCTGGGGT GTTGATGATG AGCTTGAAAA ACTCAAAGAC 
GCTGTTTCTG CCATTAAAGC CGTACTTTTG GATGCAGAGG AGCAGCAGTA CAAGAGCCAC 
GCGGTGAAGA ATTGGGTTTC GAGGCTTAAA GATGTTCTCT ACGATGTTGA CGACTTGATT 
GATGAGTTCT CTTATGAAAC CTTGAGAAGA CAAGTTAAGG CCAAAGATGG ACGAAAGCCT 
GAACAGGTAT GTATGTTCTT CTCCAAGTCA AATCCAGTTG CATTTGGTTT TAGAATGGGT 
CAAACAATAA AGGACATTAG GGAGAAATTA AATGGCATTA ATGCTGATAA AACCCAATTT 
CACTTTTCTG AGCGTACGAT GGACACGCCA GATGATGGGC TTAGGAAGGG ACGAGAGACT 
TACTCTTTTA TACCTGAAGG TGAAGTAATT GGTAGGGATG ATGACAGAAA AGCAATTGCA 
GATCTTTTGT TAGATACCAA CTTCAAAGAG GATATTGCAG TGATTGCTAT AGTTGGAATG 
GGAGGATTAG GAAAGACCAC ACTTGCTCAA TCTGTCTATA ACGATGAGAT GATAAGAAAA 
CATTTTGCAC TAAAATTGTG GGTGTGTGTT TCTGAAGAAT TTGATGTTAA AATTATTGTA 
GAAAAGATTC TGGAGTCTGC AACAATGAAG AGGCACGGGC CTTTTGAAAT AGATACATTG 
CAAAGTTTAC TTCGAAAGGA AATTGATGGA AAGAAATATC TATTCGTCAT GGATGATGTG 
TGGAATGAGA ACTATGAGAA ATGGGTCCAT TTGAAAAGAT TATTAATGTG TGGAGCAACT 
GGAAGTAGGA TTTTGATCAC CACGCGTAGT ATACAAGTTG CAAAAACATT TGATGTAGTA 
TCACTATATC CTTTAGAAAA TCTAAATGAA GACGACTCGT GGTTATTGTT TAAAAAGATG 
GCATCTTTGA AAGGAGCAGA AGAAGAGCTT GGAAATTCAA ATCTTTTCAA AATTGGCAAA 
GAGATTGTTG CAAAGTTGAA AGGTGTTCCT CTTGCAATAA GAACTATTTG GAGACTTCTA 
TGTTCCAAAA AATCAGAAGC TGACTGGTTA TTTTTCAAAA ATAATGAACT TTCAAGAGCG 
GTACGACAAG AAAATGAGAT GCAATCAATA CTTAAGATTA GTTATAACCA CCTTTCGTCT 
AATTTAAAGC AATGTTTTGC ATATTGTGCT TTATTCCCTA AAAATTATGA GATTCGAAAG 
GATGAGGTGA TAAAGCAATG GGTAGCACAA GGTTTTATTC AACCACAGAG TATTAAAGCA 
ATTGAAGATG TTGGTGAAGA TTATTTTATG GAGTTATTGT GGAGGTCGTT CTTTCAAGAT 
ATAAGGAGTA ATGAATTGGG AGACATCAAA CAGTTTAAGA TGCACGACTT AATGCATGAT 
CTTGCATGTT CGATAGATGA AAATGAATGC GTGCTTGCTA GTTTGAATGC TAGGTCTATC 
GTAAAAAGAA CTCGACACGT GTTATTTGAG TTTAAGTTAA ATGAAACGTG GGAACTTCCA 
GAATCATTAA TTGAGGCAAA GAATTTGACA ACATTGTATA ATCCTCCATT TGATCGTAAG 
AAACCCCTCC ACAAGATGTT TTCTAATCAT TTGCGATTAC GAACATTGGT TCTGGTGTAT 
AGCTTTATTA TAGAATTGCC CAAGTCCACT AGCAAGTTGA AACATTTGCG ATATCTTGAC 
CTTAGTTGTA ATAATTATAT AGAATTCCTT CCAAATTCTA TCACTGAATT GTATAATTTG 
GAAACACTTC TCCTTTGTGG TTGCACAGGT TCGAGAACAT TGCCAAGAGA TACTAAAAAT 
TTGATCAGCC TTAGACATCT TGATATGTGT GATTGTTATG CATTGACTCA TATGCCAAAA 
GGATTGGGTG AGATGACTAG TCTTCAGACA ATGAATTTAT TTGTATTAGG AGATACTAAT 
AGCAACGGAT TAAGTGAGTT GAATGGACTT GGAAATTTGA AGGGATCGTT AACTATTCGA 
GATTTACAAT TTCAGACTAT TGGTGATTTA CAAAAAAATG CTTATTACTT GAAATTAAAG 
TCAGGAATTA AACGGTTGAT GTTACATTGG AAGGATGGTC GTGGAAAGAA GTCTAAAATT 
GTTGCCTTGG ATGATGAAGA AAGAGTTTTA GAATGCTTAG AACCACATCC AAATCTTCAA 
AAGATAGAAA TATTGGGATA CCATGGAGCA AGGCTTTGTA ATTGGCTATC CTCTGATTCG 
CTTGTTCGTC TGGTCACCAT AAAACTTTCA CATTGTAGTA GATTGCAGCA GCTCCCTCAA 
TTTGATCAAT ATCCTTTTCT CAAGCATCTT TGTCTTGAAT CCTTGATCTG TATCGAGTAC 
ATTGACAACA ATAGTAATAT TTACCTTTCT TCATCAACGT TCTTCCCATC CCTTGAGAAA 
TTAGAAATTT CAACCATGCC AAACTTGAAG GGATGGTGGA AAGGCGAAAC GTCAGGGGAA 
TTAGCTCAAA ATAGTGTCTC ATTTCCAACA ATGATGCCTT GCCTTTCTAC GTGA