NELFB cDNA ORF clone, Microcebus murinus(gray mouse lemur)

Gene
Clones
gRNAs

Summary

Gene Symbol	NELFB
Entrez Gene ID	105882218
Full Name	negative elongation factor complex member B
Gene Type	protein-coding
Organism	Microcebus murinus(gray mouse lemur)

mRNA and Protein(s)

mRNA	Protein	Name
XM_020289578.1	XP_020145167.1	LOW QUALITY PROTEIN: negative elongation factor B

Pathways from BioSystems
Gene Ontology

The following NELFB gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the NELFB cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OMi57508	XM_020289578.1 Latest version!	Microcebus murinus negative elongation factor complex member B (NELFB), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$342.30 $489.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

NELFB ( XM_020289578.1 ) cDNA ORF clone, Microcebus murinus(gray mouse lemur) -> XP_020145167.1 Microcebus murinus negative elongation factor complex member B (NELFB), mRNA.

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CloneID	OMi57508
Clone ID Related Accession (Same CDS sequence)	XM_020289578.1
Accession Version	XM_020289578.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1773bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-02-23
Organism	Microcebus murinus(gray mouse lemur)
Product	LOW QUALITY PROTEIN: negative elongation factor B
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_033669.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Microcebus murinus Annotation Release 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## ##RefSeq-Attributes-START## frameshifts :: corrected 2 indels ##RefSeq-Attributes-END##

ATGTTCGCGG GGCTGCAGGA CCTGGGCGTG GCCAACGGCG AGGACCTGAA GGAGACCCTC 
ACCAACTGCA CGGAGCCGCT CAAGGCCATC GAGCAGTTCC AGACAGAGAA TGGCGTGCTG 
CTGCCCTCCC TGCAGTCGGC CTTGCCCTTC TTGGACCTAC ATGGGACGCC GCGGCTGGAG 
TTCCACCAGT CGGTGTTTGA TGAGCTGCGG GACAAACTGT TGGAGCGAGT GTCAGCCATT 
GCCTCGGAGG GGAAGGCTGA GGAAAGGTAC AAGAAGCTGG AAGACCTTCT GGAGAAGAGC 
TTTCTCTCGG TCAAGATGCC ATCTTTGCAG CCAGTGNNGA TGTGTGTCAT GAAGCACCTG 
CCCAAGGTAA GAGTCAAGGT TCCAGAGAAG AAGCTGAAGC TGGTGATGGC TGACAAGGAG 
CTGTACCGAG CCTGCGCCGT GGAGGTAGAG CGGCAGATCT GGCAAGACAA CCAGNGCCTG 
TTTGGGGACG AGGTCTCCCC ACTGCTGAAG CAGTACATCC TGGAGAAGAG AGTGGGCTCT 
TCAGCACGGA GCCTCCGTCC TGCACAACTT CTAGTCCTTC CCCAAACCAG GCGCCAGGGC 
GAGGTGGTGC GGAGGCTGAC GCAGATGGTG GGGAAGAGCG TGAAGCTGTA CGACATGGTG 
CTGCAGTTCC TGCGCACGCT CTTTCTGCGC ACGAGGAACG TGCACTACTG CACGCTGCGG 
GCCGAGCTGC TCATGTCCCT GCACGACCTG GACGTGGGCG ACATCTGCAC CGTGGACCCC 
TGCCACAAGT TCACCTGGTG CCTGGACGCC TGCATCCGAG AGCGGTTCGT GGACAGCAAG 
CGAGCCCGGG AGCTGCAGGG CTTCCTCGAC GGAGTGAAGA AGGGCCAGGA GCAGGTCCTC 
GGGGACTTGT CCATGATCCT CTGCGACCCG TTTGCCATCA ACACGCTGGC GCTGAGCACC 
ATCAGACACC TGCAGGAGCT GGTTGGCCAG GAGACGCTGC CCAGGGACAG CCCCGACCTC 
CTCCTGCTGC TCCGGCTGCT GGCGCTGGGC CAGGGTGCCT GGGACATGAT CGACAGCCAG 
GTCTTCAAGG AGCCCAAGAT GGAGGTGGAG CTCATCACCA GGTTCCTGCC GATGCTCATG 
TCCTTTGTGG TGGACGACCA CACCTTCAAC GTGGACCAGA AGCTCCCGGC TGAGGAGAAG 
GCCCCCGTCA CGTACCCCAA CACGCTTCCT GAGAGTTTCA CTAAGTTTCT GCAGGAGCAG 
CGCATGGCCT GCGAGGTGGG GCTATACTAC GTCCTGCACG TCACCAAGCA GAGGAACAAG 
AACGCGCTGC TCCGCCTGCT GCCGGGACTG GTGGAGACCT TTGGTGACCT GGCCTTCAGT 
GACATCTTCC TTCACTTGCT CACCGGGAAC CTTGCGCTTC TGGCTGATGA ATTTGCCCTG 
GAGGATTTCT GCAGCTGCCT CTTCGATGGC TTCTTCCTCT CGGCCTCACC AAGGAAGGAG 
AACGTGCACC GCCACGTGCT GCGGCTGCTC CTCCACTTGC ACCCCAGGGT GGCGCCGTCC 
AAGCTGGAGG CCCTGCAGAA GGCCCTGGAG CCCACAGGCC AGAGTGGAGA GGCCGTGAAG 
GAGCTTTACT CACAGCTCGG TGAGAAGCTG GAGCAGCTGG ACCACCGCAA GCCCAGCCCG 
GCCCAGGCTG CGGAAACGCC TGCCCTGGAG CTGCCCCTCC CCAGCGCGCC CGCCCCTGCG 
CCCGCCCCTG CCCCCGCTCC TGCCACGCTC TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_020145167.1
CDS	260..2032
Translation	MFAGLQDLGVANGEDLKETLTNCTEPLKAIEQFQTENGVLLPSLQSALPFLDLHGTPRLEFHQSVFDELRDKLLERVSAIASEGKAEERYKKLEDLLEKSFLSVKMPSLQPVnullMCVMKHLPKVRVKVPEKKLKLVMADKELYRACAVEVERQIWQDNQnullLFGDEVSPLLKQYILEKRVGSSARSLRPAQLLVLPQTRRQGEVVRRLTQMVGKSVKLYDMVLQFLRTLFLRTRNVHYCTLRAELLMSLHDLDVGDICTVDPCHKFTWCLDACIRERFVDSKRARELQGFLDGVKKGQEQVLGDLSMILCDPFAINTLALSTIRHLQELVGQETLPRDSPDLLLLLRLLALGQGAWDMIDSQVFKEPKMEVELITRFLPMLMSFVVDDHTFNVDQKLPAEEKAPVTYPNTLPESFTKFLQEQRMACEVGLYYVLHVTKQRNKNALLRLLPGLVETFGDLAFSDIFLHLLTGNLALLADEFALEDFCSCLFDGFFLSASPRKENVHRHVLRLLLHLHPRVAPSKLEALQKALEPTGQSGEAVKELYSQLGEKLEQLDHRKPSPAQAAETPALELPLPSAPAPAPAPAPAPATL

Target ORF information:

RefSeq Version	XM_020289578.1
Organism	Microcebus murinus(gray mouse lemur)
Definition	Microcebus murinus negative elongation factor complex member B (NELFB), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_020289578.1

ORF Insert Sequence:

ATGTTCGCGG GGCTGCAGGA CCTGGGCGTG GCCAACGGCG AGGACCTGAA GGAGACCCTC 
ACCAACTGCA CGGAGCCGCT CAAGGCCATC GAGCAGTTCC AGACAGAGAA TGGCGTGCTG 
CTGCCCTCCC TGCAGTCGGC CTTGCCCTTC TTGGACCTAC ATGGGACGCC GCGGCTGGAG 
TTCCACCAGT CGGTGTTTGA TGAGCTGCGG GACAAACTGT TGGAGCGAGT GTCAGCCATT 
GCCTCGGAGG GGAAGGCTGA GGAAAGGTAC AAGAAGCTGG AAGACCTTCT GGAGAAGAGC 
TTTCTCTCGG TCAAGATGCC ATCTTTGCAG CCAGTGNNGA TGTGTGTCAT GAAGCACCTG 
CCCAAGGTAA GAGTCAAGGT TCCAGAGAAG AAGCTGAAGC TGGTGATGGC TGACAAGGAG 
CTGTACCGAG CCTGCGCCGT GGAGGTAGAG CGGCAGATCT GGCAAGACAA CCAGNGCCTG 
TTTGGGGACG AGGTCTCCCC ACTGCTGAAG CAGTACATCC TGGAGAAGAG AGTGGGCTCT 
TCAGCACGGA GCCTCCGTCC TGCACAACTT CTAGTCCTTC CCCAAACCAG GCGCCAGGGC 
GAGGTGGTGC GGAGGCTGAC GCAGATGGTG GGGAAGAGCG TGAAGCTGTA CGACATGGTG 
CTGCAGTTCC TGCGCACGCT CTTTCTGCGC ACGAGGAACG TGCACTACTG CACGCTGCGG 
GCCGAGCTGC TCATGTCCCT GCACGACCTG GACGTGGGCG ACATCTGCAC CGTGGACCCC 
TGCCACAAGT TCACCTGGTG CCTGGACGCC TGCATCCGAG AGCGGTTCGT GGACAGCAAG 
CGAGCCCGGG AGCTGCAGGG CTTCCTCGAC GGAGTGAAGA AGGGCCAGGA GCAGGTCCTC 
GGGGACTTGT CCATGATCCT CTGCGACCCG TTTGCCATCA ACACGCTGGC GCTGAGCACC 
ATCAGACACC TGCAGGAGCT GGTTGGCCAG GAGACGCTGC CCAGGGACAG CCCCGACCTC 
CTCCTGCTGC TCCGGCTGCT GGCGCTGGGC CAGGGTGCCT GGGACATGAT CGACAGCCAG 
GTCTTCAAGG AGCCCAAGAT GGAGGTGGAG CTCATCACCA GGTTCCTGCC GATGCTCATG 
TCCTTTGTGG TGGACGACCA CACCTTCAAC GTGGACCAGA AGCTCCCGGC TGAGGAGAAG 
GCCCCCGTCA CGTACCCCAA CACGCTTCCT GAGAGTTTCA CTAAGTTTCT GCAGGAGCAG 
CGCATGGCCT GCGAGGTGGG GCTATACTAC GTCCTGCACG TCACCAAGCA GAGGAACAAG 
AACGCGCTGC TCCGCCTGCT GCCGGGACTG GTGGAGACCT TTGGTGACCT GGCCTTCAGT 
GACATCTTCC TTCACTTGCT CACCGGGAAC CTTGCGCTTC TGGCTGATGA ATTTGCCCTG 
GAGGATTTCT GCAGCTGCCT CTTCGATGGC TTCTTCCTCT CGGCCTCACC AAGGAAGGAG 
AACGTGCACC GCCACGTGCT GCGGCTGCTC CTCCACTTGC ACCCCAGGGT GGCGCCGTCC 
AAGCTGGAGG CCCTGCAGAA GGCCCTGGAG CCCACAGGCC AGAGTGGAGA GGCCGTGAAG 
GAGCTTTACT CACAGCTCGG TGAGAAGCTG GAGCAGCTGG ACCACCGCAA GCCCAGCCCG 
GCCCAGGCTG CGGAAACGCC TGCCCTGGAG CTGCCCCTCC CCAGCGCGCC CGCCCCTGCG 
CCCGCCCCTG CCCCCGCTCC TGCCACGCTC TGA