ORF » Species Summary » Dictyostelium discoideum AX4 » rasB cDNA ORF clone

rasB cDNA ORF clone, Dictyostelium discoideum AX4

The following rasB gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the rasB cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
ODm12280 XM_629298.1
Latest version!		 Dictyostelium discoideum AX4 Ras GTPase (rasB) mRNA, complete cds. pcDNA3.1-C-(k)DYK or customized vector 7-9 $104.30
$149.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    rasB ( XM_629298.1 ) cDNA ORF clone, Dictyostelium discoideum AX4 -> XP_629300.1 Dictyostelium discoideum AX4 Ras GTPase (rasB) mRNA, complete cds.
    Price & Availability↑
    CloneID ODm12280
    Clone ID Related Accession (Same CDS sequence) XM_629298.1
    Accession Version XM_629298.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 594bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2010-01-28
    Organism Dictyostelium discoideum AX4
    Product Ras GTPase
    Comment Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. This record is derived from an annotated genomic sequence (NC_007092). COMPLETENESS: incomplete on both ends. 

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    ATGTCAGTTT CAAATGAATA TAAATTAGTT GTTATGGGTG GTGGTGGTGT TGGTAAGAGT 
    GCACTTACTA TCCAATTTAT CCAAAATCAC TTTATTGAGG AATACGATCC AACAATAGAG 
    GACAGTTATC GTAGACAATG TCAAGTCGAT GAAGATACTT GTTTATTAGA TATTTTAGAT 
    ACTGCAGGTC AAGATGATTA CAGTGCTATG AGAGACCAAT ATATGCGTAC AGGTCAAGGT 
    TTCTTATGTG TTTATGATGT AACATCCAGA ACTTCTTTTG AAGAAATTAA TGTTGTTAGA 
    GAACAAATCA TTAGAGTAAA AGATAATGAT AAAGTTCCAA TTGTATTAGT CGGTAACAAA 
    TGTGATTTAG AAAACCTTCG TGAAGTTACT GAAGGTGAAG GAAGTGAATT GGCTAAATCT 
    TTCTCTGTTC CATTCTTGGA GACCTCTGCC AAGAAAAGAT TGAATGTGGA TGAATGTTTC 
    TTTGAAGTGG TTAGAGAAAT CAAAAAATCA CTCAAAGAGC CAGGCAGATC AAAGAAAGAT 
    AAAAAAGGTG GTATCCTTAA AAAGTTTAAA GGTGGTGATT GTTTAATCCT TTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_629300.1
    CDS1..594
    Translation

    Target ORF information:

    RefSeq Version XM_629298.1
    Organism Dictyostelium discoideum AX4
    Definition Dictyostelium discoideum AX4 Ras GTPase (rasB) mRNA, complete cds.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    		 XM_629298.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    ATGTCAGTTT CAAATGAATA TAAATTAGTT GTTATGGGTG GTGGTGGTGT TGGTAAGAGT 
    GCACTTACTA TCCAATTTAT CCAAAATCAC TTTATTGAGG AATACGATCC AACAATAGAG 
    GACAGTTATC GTAGACAATG TCAAGTCGAT GAAGATACTT GTTTATTAGA TATTTTAGAT 
    ACTGCAGGTC AAGATGATTA CAGTGCTATG AGAGACCAAT ATATGCGTAC AGGTCAAGGT 
    TTCTTATGTG TTTATGATGT AACATCCAGA ACTTCTTTTG AAGAAATTAA TGTTGTTAGA 
    GAACAAATCA TTAGAGTAAA AGATAATGAT AAAGTTCCAA TTGTATTAGT CGGTAACAAA 
    TGTGATTTAG AAAACCTTCG TGAAGTTACT GAAGGTGAAG GAAGTGAATT GGCTAAATCT 
    TTCTCTGTTC CATTCTTGGA GACCTCTGCC AAGAAAAGAT TGAATGTGGA TGAATGTTTC 
    TTTGAAGTGG TTAGAGAAAT CAAAAAATCA CTCAAAGAGC CAGGCAGATC AAAGAAAGAT 
    AAAAAAGGTG GTATCCTTAA AAAGTTTAAA GGTGGTGATT GTTTAATCCT TTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

  • PubMed

    The genome of the social amoeba Dictyostelium discoideum.
    Nature435(7038)43-57(2005 May)
    Eichinger L,Pachebat JA,Gl?ckner G,Rajandream MA,Sucgang R,Berriman M,Song J,Olsen R,Szafranski K,Xu Q,Tunggal B,Kummerfeld S,Madera M,Konfortov BA,Rivero F,Bankier AT,Lehmann R,Hamlin N,Davies R,Gaudet P,Fey P,Pilcher K,Chen G,Saunders D,Sodergren E,Davis P,Kerhornou A,Nie X,Hall N,Anjard C,Hemphill L,Bason N,Farbrother P,Desany B,Just E,Morio T,Rost R,Churcher C,Cooper J,Haydock S,van Driessche N,Cronin A,Goodhead I,Muzny D,Mourier T,Pain A,Lu M,Harper D,Lindsay R,Hauser H,James K,Quiles M,Madan Babu M,Saito T,Buchrieser C,Wardroper A,Felder M,Thangavelu M,Johnson D,Knights A,Loulseged H,Mungall K,Oliver K,Price C,Quail MA,Urushihara H,Hernandez J,Rabbinowitsch E,Steffen D,Sanders M,Ma J,Kohara Y,Sharp S,Simmonds M,Spiegler S,Tivey A,Sugano S,White B,Walker D,Woodward J,Winckler T,Tanaka Y,Shaulsky G,Schleicher M,Weinstock G,Rosenthal A,Cox EC,Chisholm RL,Gibbs R,Loomis WF,Platzer M,Kay RR,Williams J,Dear PH,Noegel AA,Barrell B,Kuspa A


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