col9a1b cDNA ORF clone, Danio rerio(zebrafish)

Gene
Clones
gRNAs

Summary

Gene Symbol	col9a1b
Entrez Gene ID	406548
Full Name	collagen, type IX, alpha 1b
Synonyms	cb339,col9a1,sb:cb339,wu:fa12a12,wu:fa12d02,wu:fc22c08,wu:fc38d08,zgc:85892
General protein information	Preferred Names collagen, type IX, alpha 1b Names collagen type IX alpha I collagen, type IX, alpha 1
Gene Type	protein-coding
Organism	Danio rerio(zebrafish)

mRNA and Protein(s)

mRNA	Protein	Name
NM_213264.2	NP_998429.2	collagen type IX alpha I precursor

Pathways from BioSystems

Homologies

Pan troglodytes (chimpanzee)	COL9A1	XP_001136406.1
Canis lupus familiaris (dog)	COL9A1	XP_538994.3
Danio rerio (zebrafish)	col9a1	NP_998429.2
Rattus norvegicus (Norway rat)	Col9a1	NP_001094312.1
Mus musculus (house mouse)	Col9a1	NP_031766.3
Danio rerio (zebrafish)	zgc:194937	NP_001124096.1
	COL9A1	XP_001111640.1
Xenopus tropicalis (tropical clawed frog)	col9a1	XP_002939444.2
Homo sapiens (human)	COL9A1	NP_001842.3
Gallus gallus (chicken)	COL9A1	NP_001094381.1

Gene Ontology
Bibliography

Related articles in PubMed

Gene evolution and gene expression after whole genome duplication in fish: the PhyloFish database.
Pasquier J, Cabau C, Nguyen T, Jouanno E, Severac D, Braasch I, Journot L, Pontarotti P, Klopp C, Postlethwait JH, Guiguen Y, Bobe J
BMC genomics17368(2016 05)

Juxtaposition of chemical and mutation-induced developmental defects in zebrafish reveal a copper-chelating activity for kalihinol F.
Sandoval IT, Manos EJ, Van Wagoner RM, Delacruz RG, Edes K, Winge DR, Ireland CM, Jones DA
Chemistry & biology20(6)753-63(2013 Jun)

Collagen IX is required for the integrity of collagen II fibrils and the regulation of vascular plexus formation in zebrafish caudal fins.
Huang CC, Wang TC, Lin BH, Wang YW, Johnson SL, Yu J
Developmental biology332(2)360-70(2009 Aug)

Expression profiling and comparative genomics identify a conserved regulatory region controlling midline expression in the zebrafish embryo.
Dickmeis T, Plessy C, Rastegar S, Aanstad P, Herwig R, Chalmel F, Fischer N, Str?hle U
Genome research14(2)228-38(2004 Feb)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Data show that mutation of collagen IXalpha1 causes a significant reduction in the size and dramatic structural defects in collagen II-rich extracellular matrices of both embryonic finfolds and adult fins.
Title: Collagen IX is required for the integrity of collagen II fibrils and the regulation of vascular plexus formation in zebrafish caudal fins.

The following col9a1b gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the col9a1b cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
ODa00908	NM_213264.2 Latest version!	Danio rerio collagen, type IX, alpha 1b (col9a1b), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$426.30 $609.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

col9a1b ( NM_213264.2 ) cDNA ORF clone, Danio rerio(zebrafish) -> NP_998429.2 Danio rerio collagen, type IX, alpha 1b (col9a1b), mRNA.

Price & Availability↑

CloneID	ODa00908
Clone ID Related Accession (Same CDS sequence)	NM_213264.2
Accession Version	NM_213264.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2046bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-11-23
Organism	Danio rerio(zebrafish)
Product	collagen type IX alpha I precursor
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from BX322620.13, BC068419.1 and CD777796.1. On Oct 17, 2008 this sequence version replaced NM_213264.1. ##Evidence-Data-START## RNAseq introns :: single sample supports all introns SAMEA3505389, SAMEA4476734 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGCTCGCG CTGCAGGTGT GCGCGGGCCC TTTTTACTCA TTTTGTTGGA GATAACCATC 
ATCTGCACGG CGCAGAGGAC TGGATCTGGG ACTCCTGGTC CTGCTGGTGT GCCTGGAATA 
GATGGCATTG ATGGGGAGAG AGGTGACACC GGTGAAGATG GCTCTGCTGG TCTTGATGGA 
GATGCTGGAA AACCTGGTTC TGCTGGACTT CCTGGATTAC CTGGAGCTGA TGGTCTCATA 
GGCCCCATTG GAGAACCAGG CCTCGACGGG CCCCCAGGAC AAAAGGGAGA ACCTGGTAAA 
CCTGGTATCA GAGGAGAGGC GGGTGTTGGA CCTGATGGAC CAATGGGCCC ACCAGGACCA 
GGAGGACTTC CGGGAGAGCT GGGAAAAGTG GGACCACCTG GAGCCATGGG TGTGAGAGGA 
CCACAGGGAC CCAGAGGACC AACAGGGCCC AGAGGAGCTG CAGGGATGTT GGGTGGATCT 
ATGGAACTGT GCCCTAATGC ATGCCCACCG GGAGCACCTG GCCATCCAGG ATTGCCTGGG 
ATGAAGGGTC ATAAAGGTGT GAAAGGAGAA TCTGGAGAGC CTGGAAAACA AGGACACAAG 
GGAGAGGAAG GGGATCAGGG AGCCTCTGGA GAGGTCGGAG CTCAAGGCCC TACTGGACCA 
CAGGGAATTC GAGGTGCTAC CGGAATGATT GGAGCAAAAG GAGAAACGGG GCCTCGGGGT 
GTTGATGGTG ACCCGGGCCC TCAAGGTGTT GCAGGGGCTG CGGGGGATCG AGGACAACGC 
GGAGGAGTGG GGGAAACCGG TCCTAAAGGA GAACAGGGAC CTCAAGGGCC AAGAGGCAAC 
ACTGGTCTTC CAGGGCCAAA AGGAGATGGC GGTTTGCCAG GAGTGGACGG ACGTGAGGGA 
ATCCCTGGCA TGCCAGGTGC CAAGGGCGAC AAAGGAAAAG CTGGAAGCCC CGGGGAAATT 
GGCCTTCAAG GACTGCCTGG TTTACCAGGA ATCCCAGGAC CAAAAGGTGG AGGAGGAGAG 
AAGGGTAACG CTGGACAACC AGGCCTTATT GGCACTATGG GATCTTCAGG CAAACAAGGT 
GAACGTGGAG AACAAGGAGA ACTCGGACCC ATTGGGCCTA TTGGACTAGC TGGAGACCGC 
GGAGAACAGG GACCCGTTGG ACCGATGGGG AAACCAGGAG CCAGAGGGCC TAAAGGTGAC 
TTGGGTTTGC CTGGACTTCC TGGACCGCCG GGCCTGCCAG GAATAAAAGG AGACCGGGGT 
GAAGGAGGTG AACCTGGACC AAAGGGAGAA CCGGGAGATC AAGGTGCAGA GGGAACACCA 
GGAGACAAGG GAGATGTCGG TGACCCCGGA CAATCTGGAG CCAAAGGCGA AGTTGGAAAC 
CAGGGTGATC CGGGTAACAG AGGGCCGCAG GGAGCTCGAG GGCAGCCTGG CATTGAGGGG 
CCTGCTGGTA GTGCTGGTCC ACGCGGCATG CAGGGCAACA GAGGAGTTCC AGGACCTCGT 
GGCTCTCAGG GACCAGCGGG CAAGGAACCG AGTGATCAAC ACATCAGACA AGTGTGCATG 
CGAGTTATGC AGGAGCAGTT GGCACAGTTG GCTGCCAGTC TGAGGAGACC AGAGTCTGGA 
GCAGTTGGAC TACCAGGCAA ACCTGGACCT CCGGGACCTC CCGGGCCCCA AGGTGACAGT 
GGTTTCCCGG GGCATGCAGG ATCCAGAGGA TTGCCGGGAC TTAAAGGGCC TCCGGGACTG 
ATGGGTGTCA AGGGGCCAAA AGGTGAGATG GGAGACAGAG GAGACAGGGG TCCAACAGTT 
CGGGGACCCA AAGGCCAGCC GGGACCTCCT GGACTACCAG GTGAACCTGG AATACCAGGA 
TATGGTCAAG ATGGACGTGA TGGAGAACGT GGTCCACCTG GATCTCCAGG TCAGCCAGGT 
GTTCCTGGAC CACCCGGTTC GGCCGGACCA CCAGGCTACT GCGATCCTTC AGCCTGTAAT 
CTAAGCGCAG GGGCCGCACA CCAATCCCTC AAAGACTCTA GCATGAAAGG ACCAGGCGGA 
AACTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_998429.2
CDS	77..2122
Translation	MARAAGVRGPFLLILLEITIICTAQRTGSGTPGPAGVPGIDGIDGERGDTGEDGSAGLDGDAGKPGSAGLPGLPGADGLIGPIGEPGLDGPPGQKGEPGKPGIRGEAGVGPDGPMGPPGPGGLPGELGKVGPPGAMGVRGPQGPRGPTGPRGAAGMLGGSMELCPNACPPGAPGHPGLPGMKGHKGVKGESGEPGKQGHKGEEGDQGASGEVGAQGPTGPQGIRGATGMIGAKGETGPRGVDGDPGPQGVAGAAGDRGQRGGVGETGPKGEQGPQGPRGNTGLPGPKGDGGLPGVDGREGIPGMPGAKGDKGKAGSPGEIGLQGLPGLPGIPGPKGGGGEKGNAGQPGLIGTMGSSGKQGERGEQGELGPIGPIGLAGDRGEQGPVGPMGKPGARGPKGDLGLPGLPGPPGLPGIKGDRGEGGEPGPKGEPGDQGAEGTPGDKGDVGDPGQSGAKGEVGNQGDPGNRGPQGARGQPGIEGPAGSAGPRGMQGNRGVPGPRGSQGPAGKEPSDQHIRQVCMRVMQEQLAQLAASLRRPESGAVGLPGKPGPPGPPGPQGDSGFPGHAGSRGLPGLKGPPGLMGVKGPKGEMGDRGDRGPTVRGPKGQPGPPGLPGEPGIPGYGQDGRDGERGPPGSPGQPGVPGPPGSAGPPGYCDPSACNLSAGAAHQSLKDSSMKGPGGN

Target ORF information:

RefSeq Version	NM_213264.2
Organism	Danio rerio(zebrafish)
Definition	Danio rerio collagen, type IX, alpha 1b (col9a1b), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_213264.2

ORF Insert Sequence:

ATGGCTCGCG CTGCAGGTGT GCGCGGGCCC TTTTTACTCA TTTTGTTGGA GATAACCATC 
ATCTGCACGG CGCAGAGGAC TGGATCTGGG ACTCCTGGTC CTGCTGGTGT GCCTGGAATA 
GATGGCATTG ATGGGGAGAG AGGTGACACC GGTGAAGATG GCTCTGCTGG TCTTGATGGA 
GATGCTGGAA AACCTGGTTC TGCTGGACTT CCTGGATTAC CTGGAGCTGA TGGTCTCATA 
GGCCCCATTG GAGAACCAGG CCTCGACGGG CCCCCAGGAC AAAAGGGAGA ACCTGGTAAA 
CCTGGTATCA GAGGAGAGGC GGGTGTTGGA CCTGATGGAC CAATGGGCCC ACCAGGACCA 
GGAGGACTTC CGGGAGAGCT GGGAAAAGTG GGACCACCTG GAGCCATGGG TGTGAGAGGA 
CCACAGGGAC CCAGAGGACC AACAGGGCCC AGAGGAGCTG CAGGGATGTT GGGTGGATCT 
ATGGAACTGT GCCCTAATGC ATGCCCACCG GGAGCACCTG GCCATCCAGG ATTGCCTGGG 
ATGAAGGGTC ATAAAGGTGT GAAAGGAGAA TCTGGAGAGC CTGGAAAACA AGGACACAAG 
GGAGAGGAAG GGGATCAGGG AGCCTCTGGA GAGGTCGGAG CTCAAGGCCC TACTGGACCA 
CAGGGAATTC GAGGTGCTAC CGGAATGATT GGAGCAAAAG GAGAAACGGG GCCTCGGGGT 
GTTGATGGTG ACCCGGGCCC TCAAGGTGTT GCAGGGGCTG CGGGGGATCG AGGACAACGC 
GGAGGAGTGG GGGAAACCGG TCCTAAAGGA GAACAGGGAC CTCAAGGGCC AAGAGGCAAC 
ACTGGTCTTC CAGGGCCAAA AGGAGATGGC GGTTTGCCAG GAGTGGACGG ACGTGAGGGA 
ATCCCTGGCA TGCCAGGTGC CAAGGGCGAC AAAGGAAAAG CTGGAAGCCC CGGGGAAATT 
GGCCTTCAAG GACTGCCTGG TTTACCAGGA ATCCCAGGAC CAAAAGGTGG AGGAGGAGAG 
AAGGGTAACG CTGGACAACC AGGCCTTATT GGCACTATGG GATCTTCAGG CAAACAAGGT 
GAACGTGGAG AACAAGGAGA ACTCGGACCC ATTGGGCCTA TTGGACTAGC TGGAGACCGC 
GGAGAACAGG GACCCGTTGG ACCGATGGGG AAACCAGGAG CCAGAGGGCC TAAAGGTGAC 
TTGGGTTTGC CTGGACTTCC TGGACCGCCG GGCCTGCCAG GAATAAAAGG AGACCGGGGT 
GAAGGAGGTG AACCTGGACC AAAGGGAGAA CCGGGAGATC AAGGTGCAGA GGGAACACCA 
GGAGACAAGG GAGATGTCGG TGACCCCGGA CAATCTGGAG CCAAAGGCGA AGTTGGAAAC 
CAGGGTGATC CGGGTAACAG AGGGCCGCAG GGAGCTCGAG GGCAGCCTGG CATTGAGGGG 
CCTGCTGGTA GTGCTGGTCC ACGCGGCATG CAGGGCAACA GAGGAGTTCC AGGACCTCGT 
GGCTCTCAGG GACCAGCGGG CAAGGAACCG AGTGATCAAC ACATCAGACA AGTGTGCATG 
CGAGTTATGC AGGAGCAGTT GGCACAGTTG GCTGCCAGTC TGAGGAGACC AGAGTCTGGA 
GCAGTTGGAC TACCAGGCAA ACCTGGACCT CCGGGACCTC CCGGGCCCCA AGGTGACAGT 
GGTTTCCCGG GGCATGCAGG ATCCAGAGGA TTGCCGGGAC TTAAAGGGCC TCCGGGACTG 
ATGGGTGTCA AGGGGCCAAA AGGTGAGATG GGAGACAGAG GAGACAGGGG TCCAACAGTT 
CGGGGACCCA AAGGCCAGCC GGGACCTCCT GGACTACCAG GTGAACCTGG AATACCAGGA 
TATGGTCAAG ATGGACGTGA TGGAGAACGT GGTCCACCTG GATCTCCAGG TCAGCCAGGT 
GTTCCTGGAC CACCCGGTTC GGCCGGACCA CCAGGCTACT GCGATCCTTC AGCCTGTAAT 
CTAAGCGCAG GGGCCGCACA CCAATCCCTC AAAGACTCTA GCATGAAAGG ACCAGGCGGA 
AACTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Gene evolution and gene expression after whole genome duplication in fish: the PhyloFish database.
BMC genomics17368(2016 05)
Pasquier J,Cabau C,Nguyen T,Jouanno E,Severac D,Braasch I,Journot L,Pontarotti P,Klopp C,Postlethwait JH,Guiguen Y,Bobe J

Juxtaposition of chemical and mutation-induced developmental defects in zebrafish reveal a copper-chelating activity for kalihinol F.
Chemistry & biology20(6)753-63(2013 Jun)
Sandoval IT,Manos EJ,Van Wagoner RM,Delacruz RG,Edes K,Winge DR,Ireland CM,Jones DA

Collagen IX is required for the integrity of collagen II fibrils and the regulation of vascular plexus formation in zebrafish caudal fins.
Developmental biology332(2)360-70(2009 Aug)
Huang CC,Wang TC,Lin BH,Wang YW,Johnson SL,Yu J

Expression profiling and comparative genomics identify a conserved regulatory region controlling midline expression in the zebrafish embryo.
Genome research14(2)228-38(2004 Feb)
Dickmeis T,Plessy C,Rastegar S,Aanstad P,Herwig R,Chalmel F,Fischer N,Str?hle U