wu:fi15d04 gene cDNA ORF clone, Danio rerio(zebrafish)

mRNA	Protein	Name
XM_021470635.1	XP_021326310.1	A-kinase anchor protein 13 isoform X7
XM_021470637.1	XP_021326312.1	A-kinase anchor protein 13 isoform X9
XM_021470641.1	XP_021326316.1	A-kinase anchor protein 13 isoform X13
XM_009297837.2	XP_009296112.1	A-kinase anchor protein 13 isoform X4
XM_009297843.2	XP_009296118.1	A-kinase anchor protein 13 isoform X10
XM_009297846.2	XP_009296121.1	A-kinase anchor protein 13 isoform X11
XM_021470631.1	XP_021326306.1	A-kinase anchor protein 13 isoform X3
XM_021470633.1	XP_021326308.1	A-kinase anchor protein 13 isoform X5
XM_009297835.2	XP_009296110.1	A-kinase anchor protein 13 isoform X5
XM_009297834.2	XP_009296109.1	A-kinase anchor protein 13 isoform X2
XM_009297836.2	XP_009296111.1	A-kinase anchor protein 13 isoform X3
XM_009297842.2	XP_009296117.1	A-kinase anchor protein 13 isoform X9
XM_021470632.1	XP_021326307.1	A-kinase anchor protein 13 isoform X4
XM_021470634.1	XP_021326309.1	A-kinase anchor protein 13 isoform X6
XM_009297833.2	XP_009296108.1	A-kinase anchor protein 13 isoform X1
XM_009297839.2	XP_009296114.1	A-kinase anchor protein 13 isoform X7
XM_009297841.2	XP_009296116.1	A-kinase anchor protein 13 isoform X8
XM_009297843.3	XP_009296118.1	A-kinase anchor protein 13 isoform X10
XM_021470638.1	XP_021326313.1	A-kinase anchor protein 13 isoform X11
XM_009297838.2	XP_009296113.1	A-kinase anchor protein 13 isoform X6
XM_009297839.3	XP_009296114.1	A-kinase anchor protein 13 isoform X1
XM_021470630.1	XP_021326305.1	A-kinase anchor protein 13 isoform X2
XM_021470636.1	XP_021326311.1	A-kinase anchor protein 13 isoform X8
XM_021470639.1	XP_021326314.1	A-kinase anchor protein 13 isoform X12

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
ODa39938	XM_021470638.1 Latest version!	Danio rerio wu:fi15d04 (wu:fi15d04), transcript variant X11, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$629.30 $899.00
ODa39939	XM_021470639.1 Latest version!	Danio rerio wu:fi15d04 (wu:fi15d04), transcript variant X12, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$629.30 $899.00
ODa39937	XM_009297843.3 Latest version!	Danio rerio wu:fi15d04 (wu:fi15d04), transcript variant X10, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$797.30 $1139.00
ODa141425	XM_021470630.1 Latest version!	Danio rerio wu:fi15d04 (wu:fi15d04), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1371.30 $1959.00
ODa141426	XM_021470631.1 Latest version!	Danio rerio wu:fi15d04 (wu:fi15d04), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1371.30 $1959.00
ODa141427	XM_021470632.1 Latest version!	Danio rerio wu:fi15d04 (wu:fi15d04), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1371.30 $1959.00
ODa141428	XM_021470633.1 Latest version!	Danio rerio wu:fi15d04 (wu:fi15d04), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1371.30 $1959.00
ODa141429	XM_021470634.1 Latest version!	Danio rerio wu:fi15d04 (wu:fi15d04), transcript variant X6, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1371.30 $1959.00
ODa141430	XM_021470635.1 Latest version!	Danio rerio wu:fi15d04 (wu:fi15d04), transcript variant X7, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1371.30 $1959.00
ODa141431	XM_021470636.1 Latest version!	Danio rerio wu:fi15d04 (wu:fi15d04), transcript variant X8, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1371.30 $1959.00
ODa141432	XM_021470637.1 Latest version!	Danio rerio wu:fi15d04 (wu:fi15d04), transcript variant X9, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1371.30 $1959.00
ODa141433	XM_021470641.1 Latest version!	Danio rerio wu:fi15d04 (wu:fi15d04), transcript variant X13, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1371.30 $1959.00
ODa39927	XM_009297833.2 Latest version!	Danio rerio wu:fi15d04 (wu:fi15d04), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	30	$1371.30 $1959.00
ODa39928	XM_009297834.2 Latest version!	Danio rerio wu:fi15d04 (wu:fi15d04), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	30	$1371.30 $1959.00
ODa39929	XM_009297835.2 Latest version!	Danio rerio wu:fi15d04 (wu:fi15d04), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	30	$1371.30 $1959.00
ODa39930	XM_009297836.2 Latest version!	Danio rerio wu:fi15d04 (wu:fi15d04), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	30	$1371.30 $1959.00
ODa39931	XM_009297837.2 Latest version!	Danio rerio wu:fi15d04 (wu:fi15d04), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	30	$1371.30 $1959.00
ODa39932	XM_009297838.2 Latest version!	Danio rerio wu:fi15d04 (wu:fi15d04), transcript variant X6, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	30	$1371.30 $1959.00
ODa39933	XM_009297839.3 Latest version!	Danio rerio wu:fi15d04 (wu:fi15d04), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	30	$1371.30 $1959.00
ODa39935	XM_009297841.2 Latest version!	Danio rerio wu:fi15d04 (wu:fi15d04), transcript variant X8, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	30	$1371.30 $1959.00
ODa39936	XM_009297842.2 Latest version!	Danio rerio wu:fi15d04 (wu:fi15d04), transcript variant X9, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	30	$1371.30 $1959.00
ODa39940	XM_009297846.2 Latest version!	Danio rerio wu:fi15d04 (wu:fi15d04), transcript variant X11, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	30	$1371.30 $1959.00
View Old Accession Versions >>
ODa39937	XM_009297843.2	Danio rerio wu:fi15d04 (wu:fi15d04), transcript variant X10, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$797.30 $1139.00
ODa39933	XM_009297839.2	Danio rerio wu:fi15d04 (wu:fi15d04), transcript variant X7, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	30	$1371.30 $1959.00
Hide Old Accession Versions >>

wu:fi15d04 ( XM_021470638.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_021326313.1 Danio rerio wu:fi15d04 (wu:fi15d04), transcript variant X11, mRNA.

Price & Availability↑

CloneID	ODa39938
Clone ID Related Accession (Same CDS sequence)	XM_021470638.1
Accession Version	XM_021470638.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3210bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	A-kinase anchor protein 13 isoform X11
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007136.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCTACGT TGTGTCGTAG GTTTTTACCG CAGGGTCGGT CACTGAAGAT CAAGAATACT 
GTTGGTGTGG TTGAGCAGGA GAAGGAGAAA GAGAAGAATA AGGAGCGTGA GCGAGATGCC 
AAAGACAAGG AGAGAAGAGC CTCAAACGGA CATCTCTTCA CTACGGTCAT GACTGTACCC 
ACAATCCCCT GCCACCAGTG CAACAAACTC ATCAACACTA AAGACTCTAT CCTCTGCACC 
AATTGCAATG CACAAGTCCA CAAAGGCTGC AAAGAGAGTC TTCCTGTCTG CGCTAAAGTC 
AAGATGAAGC AGCAAAAACA GCAGCAGACT GTTACAGATT CTGCCTCGAC GCCTAGTGTC 
ACCCTCAGAA GTAAAACTCT TCCAGCAAGG GATCGTCCGT GGTCCACAAT ATCCATTCCC 
GATGACCAGC CGGCTCCCCT CGCTCCTCCT CGGAAAAGCC CCAGCAGCAT CATGTCTTTC 
AACAGCAACC CACTGTCCAA GAGCATGTCC ATTAATAACA TAGCAGGACA AATGGACGAC 
ACTCCTCTTA AGAAGTTTCT GTCCCAGTCA ACGGACTCTC TTCACAAGAC CAGTAAAGTC 
ACGGAGTCTA CAGAATCTCT TACTGATGAG GGTACAGAGA TGATGGACAG CCAGCTGATG 
GGAGAATTTG AAGCTGACGC CAAAGAGCTG GAGGCTGATT CTTATAGCTT CACGGTAGAC 
AAGAAATTTC TTAAGCAGCT TAAAAAGGAT GTTATCAAAA GGCAAGACGT CATCTATGAG 
CTCATCCAGA CAGAAATGCA CCATGTAAGG ACCTTAAAGA TCATGGCTGA TGTTTACAGC 
AAAGGACTAC TGAGAGAAGT TCAACTAGAA GCTCAGACGG TGGAGAAGAT GTTCCCCATG 
TTGGACGATG TTCTGGATTT GCACACACAG TTCTTTTCAC ACCTGCTTGA AAGAAAGAAG 
GAATCAGGGA GCCAAGAAGA GGGCGGCTTT GTCATCCAAA GGATTGGCGA TGTGCTGGTT 
TCTCAGTTCT CAGGTTCCAG TGCTGAGAGA ATGAAGAAGG TCTATGGGAA GTTCTGCGGC 
AGGCACAATG AAGCTGTGAA CTTCTACAAG GAGCTTCACA CCAAAGACAA ACGCTTTCAA 
GCTTTCATCA AGAAAAAGAT GAGCAGCCCT GTTGTCCGAC GGCTAAGCAT TCCTGAGTGT 
ATATTGTTAG TCACACAACG AATCACAAAA TATCCCGTGT TACTACAGAG AATACTGCAG 
CACACGAAAG AAAACGAGGA GGACCATGCA GACGTTAGTC AAGGTCTGAA GCTGGTGAAG 
GAGGTGATTG CTGCCGTGGA CAATAAAGTG AATGAGCATG AGAAGAAGAA GAGGCTAAAG 
GAAGTGTACA GCCGGACGGA CAACAAGTCC ATCATGAGGA TGAAGAGCGG GCAGATGTTT 
GCCAGAGAGG ATCTGTTACG TGGGCGGAAG TTAATTCACG ACGGGCCGCT GCAGCTCAAG 
AACTCAGCTG GCAGACTCAA GGATGTCCAG GCTCTGCTGC TGAGGGACGT GATTGTGTTC 
CTGCAGGAGA AAGATCAGAA GTACATTTTC GCCTCTTTGG ACCAGCGCTC CACCGTCATC 
TCCCTGCAGA AGCTCATTGT GAGGGAAGTA GCCAATGAGG AGAAAGCTCT GTTTCTGATC 
ACAGCAGGCA TCGAGAAGCC CGAGATGGTA GAAGTGTACA CAAGCTCCAA AGAGGAGAGA 
AACACATGGA TGCAGCTCAT TCAGGAGGCC ATGCAGTCTA TACTGTCCAT TTCCTCCAGG 
GAGAGAGATG ATGATGAAGG TGTGCCAAGT GAAAACGAAG AGGACAGGAG ACTTCAGGAA 
ATCAAGGTCA AGGAGTTCAG AGATCAGCTG CACAAAAGAG ACGAGCAGAT CTTGACGTTA 
CTGGAGGAGA AGGTGAAGTT GTTCAGGGAC ATGTGTGACT GTGGTGCACC GGACGAGACG 
GGGCTTCGTA ATCGAATGCT CTTCAGGGCC ACGACGGATG ATGTCACCAG AGGAGAGCCA 
ATCATGAAGG AGGCTCTGAA GGAAGTTGAA ACGCTGCAGG TTCTGGTTAA TGACAGTCTT 
GGCGGTGCGG TTCAGGAAGG TGTGTGCGCG GCTGGACATG TGACTTTGCC ACGACGAGCG 
GAAACATTTG GAGGTTTCGA CAGTCACCAG ATGAACATCT CTAAGAGCAA AGATGGAGAC 
AGAGAGGAGC TGGAGGAGTC TGCAGAGCTC CGCAGGACTG AGTCAGACAG TGTGTTGAAG 
AAGACGAGTA AGACCAACCC TTTACTGCTG GTCAGGAGAA ACAATGAGCA GGTTCTTCAG 
AGCGTTGCTC ATCTTCATGA CCTGCTGAAC ACATTACAAG CTGTGGTGGT CCAGCAGGAC 
ACCTTCATCG AAGACCAGCG TCACGCTCAT CCCTCCTCAC GCCACCCATC CATCTCCTCC 
TCTACCTCCT CTTTATCATC CACCTCATCC CGGCCGAGCT CTCTAATCGA GCAGGAGAAA 
CAGCGCAGCC TGGAGAAGCA GCGGCAGGAG GTGGCCAGCC TCCAAAAACA GCAGGCGGCC 
CACGCCGAGG AGAGGCGGCG CAGGGAAAAG CAGTGGGAGA CCCGAGAGAA AGAGCTAACC 
CAGAGGGAGA TGCAGATCCA GAGCCAAGAA GAGGAGGCGC AGAGGAGAGC CAGACAGCTG 
GAGGAGGAGA AACAGGAGCT GCAGAGTAAG AAAGAGGAGT ATCAGAGAGA CCTGGCACGA 
CTGCGGGACA GCCAGAAGAG ACTGGAGAGA GAGAGAGAGC AGGTGCAGAG AGAGGCAGAT 
GAGCTTAAAC TGACTGAGGA AGTGACAAAG AACCGGTCTC CGTCAACCAC GTCTGAAGAC 
TCCACGAAGT TCCAGAGCAC CGGTTCATTA GACCGTGACC CATCAGAGAT GGAGCTCTCC 
TCCTCCCCGT CAGCCAGAGA CTTCCTGTCC CGCATGGACT CCAAACGCAA AGGGAAAAAC 
CTCAATCCAT TCTCATCCAA CTCAAGCCAG AAGAGCCAGA ACAGCGAGGC TCAGAGCCAG 
ATGCCCAGCC GTCTGCTCCA GCTGGCCAAA CCCAAGGAGA AGAAGGACAA GAAGAAGAAG 
AAGGGGAAAG GCCAGCAGAG CCAGACTGCA GACTCTCAGA GCACTGTTGT TCCAGGCGCT 
ACCCCAGACG GAGAGATCTT TTTCTGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_021326313.1
CDS	151..3360
Translation	MSTLCRRFLPQGRSLKIKNTVGVVEQEKEKEKNKERERDAKDKERRASNGHLFTTVMTVPTIPCHQCNKLINTKDSILCTNCNAQVHKGCKESLPVCAKVKMKQQKQQQTVTDSASTPSVTLRSKTLPARDRPWSTISIPDDQPAPLAPPRKSPSSIMSFNSNPLSKSMSINNIAGQMDDTPLKKFLSQSTDSLHKTSKVTESTESLTDEGTEMMDSQLMGEFEADAKELEADSYSFTVDKKFLKQLKKDVIKRQDVIYELIQTEMHHVRTLKIMADVYSKGLLREVQLEAQTVEKMFPMLDDVLDLHTQFFSHLLERKKESGSQEEGGFVIQRIGDVLVSQFSGSSAERMKKVYGKFCGRHNEAVNFYKELHTKDKRFQAFIKKKMSSPVVRRLSIPECILLVTQRITKYPVLLQRILQHTKENEEDHADVSQGLKLVKEVIAAVDNKVNEHEKKKRLKEVYSRTDNKSIMRMKSGQMFAREDLLRGRKLIHDGPLQLKNSAGRLKDVQALLLRDVIVFLQEKDQKYIFASLDQRSTVISLQKLIVREVANEEKALFLITAGIEKPEMVEVYTSSKEERNTWMQLIQEAMQSILSISSRERDDDEGVPSENEEDRRLQEIKVKEFRDQLHKRDEQILTLLEEKVKLFRDMCDCGAPDETGLRNRMLFRATTDDVTRGEPIMKEALKEVETLQVLVNDSLGGAVQEGVCAAGHVTLPRRAETFGGFDSHQMNISKSKDGDREELEESAELRRTESDSVLKKTSKTNPLLLVRRNNEQVLQSVAHLHDLLNTLQAVVVQQDTFIEDQRHAHPSSRHPSISSSTSSLSSTSSRPSSLIEQEKQRSLEKQRQEVASLQKQQAAHAEERRRREKQWETREKELTQREMQIQSQEEEAQRRARQLEEEKQELQSKKEEYQRDLARLRDSQKRLEREREQVQREADELKLTEEVTKNRSPSTTSEDSTKFQSTGSLDRDPSEMELSSSPSARDFLSRMDSKRKGKNLNPFSSNSSQKSQNSEAQSQMPSRLLQLAKPKEKKDKKKKKGKGQQSQTADSQSTVVPGATPDGEIFFC

Target ORF information:

RefSeq Version	XM_021470638.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio wu:fi15d04 (wu:fi15d04), transcript variant X11, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_021470638.1

ORF Insert Sequence:

ATGTCTACGT TGTGTCGTAG GTTTTTACCG CAGGGTCGGT CACTGAAGAT CAAGAATACT 
GTTGGTGTGG TTGAGCAGGA GAAGGAGAAA GAGAAGAATA AGGAGCGTGA GCGAGATGCC 
AAAGACAAGG AGAGAAGAGC CTCAAACGGA CATCTCTTCA CTACGGTCAT GACTGTACCC 
ACAATCCCCT GCCACCAGTG CAACAAACTC ATCAACACTA AAGACTCTAT CCTCTGCACC 
AATTGCAATG CACAAGTCCA CAAAGGCTGC AAAGAGAGTC TTCCTGTCTG CGCTAAAGTC 
AAGATGAAGC AGCAAAAACA GCAGCAGACT GTTACAGATT CTGCCTCGAC GCCTAGTGTC 
ACCCTCAGAA GTAAAACTCT TCCAGCAAGG GATCGTCCGT GGTCCACAAT ATCCATTCCC 
GATGACCAGC CGGCTCCCCT CGCTCCTCCT CGGAAAAGCC CCAGCAGCAT CATGTCTTTC 
AACAGCAACC CACTGTCCAA GAGCATGTCC ATTAATAACA TAGCAGGACA AATGGACGAC 
ACTCCTCTTA AGAAGTTTCT GTCCCAGTCA ACGGACTCTC TTCACAAGAC CAGTAAAGTC 
ACGGAGTCTA CAGAATCTCT TACTGATGAG GGTACAGAGA TGATGGACAG CCAGCTGATG 
GGAGAATTTG AAGCTGACGC CAAAGAGCTG GAGGCTGATT CTTATAGCTT CACGGTAGAC 
AAGAAATTTC TTAAGCAGCT TAAAAAGGAT GTTATCAAAA GGCAAGACGT CATCTATGAG 
CTCATCCAGA CAGAAATGCA CCATGTAAGG ACCTTAAAGA TCATGGCTGA TGTTTACAGC 
AAAGGACTAC TGAGAGAAGT TCAACTAGAA GCTCAGACGG TGGAGAAGAT GTTCCCCATG 
TTGGACGATG TTCTGGATTT GCACACACAG TTCTTTTCAC ACCTGCTTGA AAGAAAGAAG 
GAATCAGGGA GCCAAGAAGA GGGCGGCTTT GTCATCCAAA GGATTGGCGA TGTGCTGGTT 
TCTCAGTTCT CAGGTTCCAG TGCTGAGAGA ATGAAGAAGG TCTATGGGAA GTTCTGCGGC 
AGGCACAATG AAGCTGTGAA CTTCTACAAG GAGCTTCACA CCAAAGACAA ACGCTTTCAA 
GCTTTCATCA AGAAAAAGAT GAGCAGCCCT GTTGTCCGAC GGCTAAGCAT TCCTGAGTGT 
ATATTGTTAG TCACACAACG AATCACAAAA TATCCCGTGT TACTACAGAG AATACTGCAG 
CACACGAAAG AAAACGAGGA GGACCATGCA GACGTTAGTC AAGGTCTGAA GCTGGTGAAG 
GAGGTGATTG CTGCCGTGGA CAATAAAGTG AATGAGCATG AGAAGAAGAA GAGGCTAAAG 
GAAGTGTACA GCCGGACGGA CAACAAGTCC ATCATGAGGA TGAAGAGCGG GCAGATGTTT 
GCCAGAGAGG ATCTGTTACG TGGGCGGAAG TTAATTCACG ACGGGCCGCT GCAGCTCAAG 
AACTCAGCTG GCAGACTCAA GGATGTCCAG GCTCTGCTGC TGAGGGACGT GATTGTGTTC 
CTGCAGGAGA AAGATCAGAA GTACATTTTC GCCTCTTTGG ACCAGCGCTC CACCGTCATC 
TCCCTGCAGA AGCTCATTGT GAGGGAAGTA GCCAATGAGG AGAAAGCTCT GTTTCTGATC 
ACAGCAGGCA TCGAGAAGCC CGAGATGGTA GAAGTGTACA CAAGCTCCAA AGAGGAGAGA 
AACACATGGA TGCAGCTCAT TCAGGAGGCC ATGCAGTCTA TACTGTCCAT TTCCTCCAGG 
GAGAGAGATG ATGATGAAGG TGTGCCAAGT GAAAACGAAG AGGACAGGAG ACTTCAGGAA 
ATCAAGGTCA AGGAGTTCAG AGATCAGCTG CACAAAAGAG ACGAGCAGAT CTTGACGTTA 
CTGGAGGAGA AGGTGAAGTT GTTCAGGGAC ATGTGTGACT GTGGTGCACC GGACGAGACG 
GGGCTTCGTA ATCGAATGCT CTTCAGGGCC ACGACGGATG ATGTCACCAG AGGAGAGCCA 
ATCATGAAGG AGGCTCTGAA GGAAGTTGAA ACGCTGCAGG TTCTGGTTAA TGACAGTCTT 
GGCGGTGCGG TTCAGGAAGG TGTGTGCGCG GCTGGACATG TGACTTTGCC ACGACGAGCG 
GAAACATTTG GAGGTTTCGA CAGTCACCAG ATGAACATCT CTAAGAGCAA AGATGGAGAC 
AGAGAGGAGC TGGAGGAGTC TGCAGAGCTC CGCAGGACTG AGTCAGACAG TGTGTTGAAG 
AAGACGAGTA AGACCAACCC TTTACTGCTG GTCAGGAGAA ACAATGAGCA GGTTCTTCAG 
AGCGTTGCTC ATCTTCATGA CCTGCTGAAC ACATTACAAG CTGTGGTGGT CCAGCAGGAC 
ACCTTCATCG AAGACCAGCG TCACGCTCAT CCCTCCTCAC GCCACCCATC CATCTCCTCC 
TCTACCTCCT CTTTATCATC CACCTCATCC CGGCCGAGCT CTCTAATCGA GCAGGAGAAA 
CAGCGCAGCC TGGAGAAGCA GCGGCAGGAG GTGGCCAGCC TCCAAAAACA GCAGGCGGCC 
CACGCCGAGG AGAGGCGGCG CAGGGAAAAG CAGTGGGAGA CCCGAGAGAA AGAGCTAACC 
CAGAGGGAGA TGCAGATCCA GAGCCAAGAA GAGGAGGCGC AGAGGAGAGC CAGACAGCTG 
GAGGAGGAGA AACAGGAGCT GCAGAGTAAG AAAGAGGAGT ATCAGAGAGA CCTGGCACGA 
CTGCGGGACA GCCAGAAGAG ACTGGAGAGA GAGAGAGAGC AGGTGCAGAG AGAGGCAGAT 
GAGCTTAAAC TGACTGAGGA AGTGACAAAG AACCGGTCTC CGTCAACCAC GTCTGAAGAC 
TCCACGAAGT TCCAGAGCAC CGGTTCATTA GACCGTGACC CATCAGAGAT GGAGCTCTCC 
TCCTCCCCGT CAGCCAGAGA CTTCCTGTCC CGCATGGACT CCAAACGCAA AGGGAAAAAC 
CTCAATCCAT TCTCATCCAA CTCAAGCCAG AAGAGCCAGA ACAGCGAGGC TCAGAGCCAG 
ATGCCCAGCC GTCTGCTCCA GCTGGCCAAA CCCAAGGAGA AGAAGGACAA GAAGAAGAAG 
AAGGGGAAAG GCCAGCAGAG CCAGACTGCA GACTCTCAGA GCACTGTTGT TCCAGGCGCT 
ACCCCAGACG GAGAGATCTT TTTCTGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

wu:fi15d04 ( XM_021470639.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_021326314.1 Danio rerio wu:fi15d04 (wu:fi15d04), transcript variant X12, mRNA.

Price & Availability↑

CloneID	ODa39939
Clone ID Related Accession (Same CDS sequence)	XM_021470639.1
Accession Version	XM_021470639.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3207bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	A-kinase anchor protein 13 isoform X12
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007136.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCTACGT TGTGTCGTAG GTTTTTACCG CAGGGTCGGT CACTGAAGAT CAAGAATACT 
GTTGGTGTGG TTGAGGAGAA GGAGAAAGAG AAGAATAAGG AGCGTGAGCG AGATGCCAAA 
GACAAGGAGA GAAGAGCCTC AAACGGACAT CTCTTCACTA CGGTCATGAC TGTACCCACA 
ATCCCCTGCC ACCAGTGCAA CAAACTCATC AACACTAAAG ACTCTATCCT CTGCACCAAT 
TGCAATGCAC AAGTCCACAA AGGCTGCAAA GAGAGTCTTC CTGTCTGCGC TAAAGTCAAG 
ATGAAGCAGC AAAAACAGCA GCAGACTGTT ACAGATTCTG CCTCGACGCC TAGTGTCACC 
CTCAGAAGTA AAACTCTTCC AGCAAGGGAT CGTCCGTGGT CCACAATATC CATTCCCGAT 
GACCAGCCGG CTCCCCTCGC TCCTCCTCGG AAAAGCCCCA GCAGCATCAT GTCTTTCAAC 
AGCAACCCAC TGTCCAAGAG CATGTCCATT AATAACATAG CAGGACAAAT GGACGACACT 
CCTCTTAAGA AGTTTCTGTC CCAGTCAACG GACTCTCTTC ACAAGACCAG TAAAGTCACG 
GAGTCTACAG AATCTCTTAC TGATGAGGGT ACAGAGATGA TGGACAGCCA GCTGATGGGA 
GAATTTGAAG CTGACGCCAA AGAGCTGGAG GCTGATTCTT ATAGCTTCAC GGTAGACAAG 
AAATTTCTTA AGCAGCTTAA AAAGGATGTT ATCAAAAGGC AAGACGTCAT CTATGAGCTC 
ATCCAGACAG AAATGCACCA TGTAAGGACC TTAAAGATCA TGGCTGATGT TTACAGCAAA 
GGACTACTGA GAGAAGTTCA ACTAGAAGCT CAGACGGTGG AGAAGATGTT CCCCATGTTG 
GACGATGTTC TGGATTTGCA CACACAGTTC TTTTCACACC TGCTTGAAAG AAAGAAGGAA 
TCAGGGAGCC AAGAAGAGGG CGGCTTTGTC ATCCAAAGGA TTGGCGATGT GCTGGTTTCT 
CAGTTCTCAG GTTCCAGTGC TGAGAGAATG AAGAAGGTCT ATGGGAAGTT CTGCGGCAGG 
CACAATGAAG CTGTGAACTT CTACAAGGAG CTTCACACCA AAGACAAACG CTTTCAAGCT 
TTCATCAAGA AAAAGATGAG CAGCCCTGTT GTCCGACGGC TAAGCATTCC TGAGTGTATA 
TTGTTAGTCA CACAACGAAT CACAAAATAT CCCGTGTTAC TACAGAGAAT ACTGCAGCAC 
ACGAAAGAAA ACGAGGAGGA CCATGCAGAC GTTAGTCAAG GTCTGAAGCT GGTGAAGGAG 
GTGATTGCTG CCGTGGACAA TAAAGTGAAT GAGCATGAGA AGAAGAAGAG GCTAAAGGAA 
GTGTACAGCC GGACGGACAA CAAGTCCATC ATGAGGATGA AGAGCGGGCA GATGTTTGCC 
AGAGAGGATC TGTTACGTGG GCGGAAGTTA ATTCACGACG GGCCGCTGCA GCTCAAGAAC 
TCAGCTGGCA GACTCAAGGA TGTCCAGGCT CTGCTGCTGA GGGACGTGAT TGTGTTCCTG 
CAGGAGAAAG ATCAGAAGTA CATTTTCGCC TCTTTGGACC AGCGCTCCAC CGTCATCTCC 
CTGCAGAAGC TCATTGTGAG GGAAGTAGCC AATGAGGAGA AAGCTCTGTT TCTGATCACA 
GCAGGCATCG AGAAGCCCGA GATGGTAGAA GTGTACACAA GCTCCAAAGA GGAGAGAAAC 
ACATGGATGC AGCTCATTCA GGAGGCCATG CAGTCTATAC TGTCCATTTC CTCCAGGGAG 
AGAGATGATG ATGAAGGTGT GCCAAGTGAA AACGAAGAGG ACAGGAGACT TCAGGAAATC 
AAGGTCAAGG AGTTCAGAGA TCAGCTGCAC AAAAGAGACG AGCAGATCTT GACGTTACTG 
GAGGAGAAGG TGAAGTTGTT CAGGGACATG TGTGACTGTG GTGCACCGGA CGAGACGGGG 
CTTCGTAATC GAATGCTCTT CAGGGCCACG ACGGATGATG TCACCAGAGG AGAGCCAATC 
ATGAAGGAGG CTCTGAAGGA AGTTGAAACG CTGCAGGTTC TGGTTAATGA CAGTCTTGGC 
GGTGCGGTTC AGGAAGGTGT GTGCGCGGCT GGACATGTGA CTTTGCCACG ACGAGCGGAA 
ACATTTGGAG GTTTCGACAG TCACCAGATG AACATCTCTA AGAGCAAAGA TGGAGACAGA 
GAGGAGCTGG AGGAGTCTGC AGAGCTCCGC AGGACTGAGT CAGACAGTGT GTTGAAGAAG 
ACGAGTAAGA CCAACCCTTT ACTGCTGGTC AGGAGAAACA ATGAGCAGGT TCTTCAGAGC 
GTTGCTCATC TTCATGACCT GCTGAACACA TTACAAGCTG TGGTGGTCCA GCAGGACACC 
TTCATCGAAG ACCAGCGTCA CGCTCATCCC TCCTCACGCC ACCCATCCAT CTCCTCCTCT 
ACCTCCTCTT TATCATCCAC CTCATCCCGG CCGAGCTCTC TAATCGAGCA GGAGAAACAG 
CGCAGCCTGG AGAAGCAGCG GCAGGAGGTG GCCAGCCTCC AAAAACAGCA GGCGGCCCAC 
GCCGAGGAGA GGCGGCGCAG GGAAAAGCAG TGGGAGACCC GAGAGAAAGA GCTAACCCAG 
AGGGAGATGC AGATCCAGAG CCAAGAAGAG GAGGCGCAGA GGAGAGCCAG ACAGCTGGAG 
GAGGAGAAAC AGGAGCTGCA GAGTAAGAAA GAGGAGTATC AGAGAGACCT GGCACGACTG 
CGGGACAGCC AGAAGAGACT GGAGAGAGAG AGAGAGCAGG TGCAGAGAGA GGCAGATGAG 
CTTAAACTGA CTGAGGAAGT GACAAAGAAC CGGTCTCCGT CAACCACGTC TGAAGACTCC 
ACGAAGTTCC AGAGCACCGG TTCATTAGAC CGTGACCCAT CAGAGATGGA GCTCTCCTCC 
TCCCCGTCAG CCAGAGACTT CCTGTCCCGC ATGGACTCCA AACGCAAAGG GAAAAACCTC 
AATCCATTCT CATCCAACTC AAGCCAGAAG AGCCAGAACA GCGAGGCTCA GAGCCAGATG 
CCCAGCCGTC TGCTCCAGCT GGCCAAACCC AAGGAGAAGA AGGACAAGAA GAAGAAGAAG 
GGGAAAGGCC AGCAGAGCCA GACTGCAGAC TCTCAGAGCA CTGTTGTTCC AGGCGCTACC 
CCAGACGGAG AGATCTTTTT CTGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_021326314.1
CDS	151..3357
Translation	MSTLCRRFLPQGRSLKIKNTVGVVEEKEKEKNKERERDAKDKERRASNGHLFTTVMTVPTIPCHQCNKLINTKDSILCTNCNAQVHKGCKESLPVCAKVKMKQQKQQQTVTDSASTPSVTLRSKTLPARDRPWSTISIPDDQPAPLAPPRKSPSSIMSFNSNPLSKSMSINNIAGQMDDTPLKKFLSQSTDSLHKTSKVTESTESLTDEGTEMMDSQLMGEFEADAKELEADSYSFTVDKKFLKQLKKDVIKRQDVIYELIQTEMHHVRTLKIMADVYSKGLLREVQLEAQTVEKMFPMLDDVLDLHTQFFSHLLERKKESGSQEEGGFVIQRIGDVLVSQFSGSSAERMKKVYGKFCGRHNEAVNFYKELHTKDKRFQAFIKKKMSSPVVRRLSIPECILLVTQRITKYPVLLQRILQHTKENEEDHADVSQGLKLVKEVIAAVDNKVNEHEKKKRLKEVYSRTDNKSIMRMKSGQMFAREDLLRGRKLIHDGPLQLKNSAGRLKDVQALLLRDVIVFLQEKDQKYIFASLDQRSTVISLQKLIVREVANEEKALFLITAGIEKPEMVEVYTSSKEERNTWMQLIQEAMQSILSISSRERDDDEGVPSENEEDRRLQEIKVKEFRDQLHKRDEQILTLLEEKVKLFRDMCDCGAPDETGLRNRMLFRATTDDVTRGEPIMKEALKEVETLQVLVNDSLGGAVQEGVCAAGHVTLPRRAETFGGFDSHQMNISKSKDGDREELEESAELRRTESDSVLKKTSKTNPLLLVRRNNEQVLQSVAHLHDLLNTLQAVVVQQDTFIEDQRHAHPSSRHPSISSSTSSLSSTSSRPSSLIEQEKQRSLEKQRQEVASLQKQQAAHAEERRRREKQWETREKELTQREMQIQSQEEEAQRRARQLEEEKQELQSKKEEYQRDLARLRDSQKRLEREREQVQREADELKLTEEVTKNRSPSTTSEDSTKFQSTGSLDRDPSEMELSSSPSARDFLSRMDSKRKGKNLNPFSSNSSQKSQNSEAQSQMPSRLLQLAKPKEKKDKKKKKGKGQQSQTADSQSTVVPGATPDGEIFFC

Target ORF information:

RefSeq Version	XM_021470639.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio wu:fi15d04 (wu:fi15d04), transcript variant X12, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_021470639.1

ORF Insert Sequence:

ATGTCTACGT TGTGTCGTAG GTTTTTACCG CAGGGTCGGT CACTGAAGAT CAAGAATACT 
GTTGGTGTGG TTGAGGAGAA GGAGAAAGAG AAGAATAAGG AGCGTGAGCG AGATGCCAAA 
GACAAGGAGA GAAGAGCCTC AAACGGACAT CTCTTCACTA CGGTCATGAC TGTACCCACA 
ATCCCCTGCC ACCAGTGCAA CAAACTCATC AACACTAAAG ACTCTATCCT CTGCACCAAT 
TGCAATGCAC AAGTCCACAA AGGCTGCAAA GAGAGTCTTC CTGTCTGCGC TAAAGTCAAG 
ATGAAGCAGC AAAAACAGCA GCAGACTGTT ACAGATTCTG CCTCGACGCC TAGTGTCACC 
CTCAGAAGTA AAACTCTTCC AGCAAGGGAT CGTCCGTGGT CCACAATATC CATTCCCGAT 
GACCAGCCGG CTCCCCTCGC TCCTCCTCGG AAAAGCCCCA GCAGCATCAT GTCTTTCAAC 
AGCAACCCAC TGTCCAAGAG CATGTCCATT AATAACATAG CAGGACAAAT GGACGACACT 
CCTCTTAAGA AGTTTCTGTC CCAGTCAACG GACTCTCTTC ACAAGACCAG TAAAGTCACG 
GAGTCTACAG AATCTCTTAC TGATGAGGGT ACAGAGATGA TGGACAGCCA GCTGATGGGA 
GAATTTGAAG CTGACGCCAA AGAGCTGGAG GCTGATTCTT ATAGCTTCAC GGTAGACAAG 
AAATTTCTTA AGCAGCTTAA AAAGGATGTT ATCAAAAGGC AAGACGTCAT CTATGAGCTC 
ATCCAGACAG AAATGCACCA TGTAAGGACC TTAAAGATCA TGGCTGATGT TTACAGCAAA 
GGACTACTGA GAGAAGTTCA ACTAGAAGCT CAGACGGTGG AGAAGATGTT CCCCATGTTG 
GACGATGTTC TGGATTTGCA CACACAGTTC TTTTCACACC TGCTTGAAAG AAAGAAGGAA 
TCAGGGAGCC AAGAAGAGGG CGGCTTTGTC ATCCAAAGGA TTGGCGATGT GCTGGTTTCT 
CAGTTCTCAG GTTCCAGTGC TGAGAGAATG AAGAAGGTCT ATGGGAAGTT CTGCGGCAGG 
CACAATGAAG CTGTGAACTT CTACAAGGAG CTTCACACCA AAGACAAACG CTTTCAAGCT 
TTCATCAAGA AAAAGATGAG CAGCCCTGTT GTCCGACGGC TAAGCATTCC TGAGTGTATA 
TTGTTAGTCA CACAACGAAT CACAAAATAT CCCGTGTTAC TACAGAGAAT ACTGCAGCAC 
ACGAAAGAAA ACGAGGAGGA CCATGCAGAC GTTAGTCAAG GTCTGAAGCT GGTGAAGGAG 
GTGATTGCTG CCGTGGACAA TAAAGTGAAT GAGCATGAGA AGAAGAAGAG GCTAAAGGAA 
GTGTACAGCC GGACGGACAA CAAGTCCATC ATGAGGATGA AGAGCGGGCA GATGTTTGCC 
AGAGAGGATC TGTTACGTGG GCGGAAGTTA ATTCACGACG GGCCGCTGCA GCTCAAGAAC 
TCAGCTGGCA GACTCAAGGA TGTCCAGGCT CTGCTGCTGA GGGACGTGAT TGTGTTCCTG 
CAGGAGAAAG ATCAGAAGTA CATTTTCGCC TCTTTGGACC AGCGCTCCAC CGTCATCTCC 
CTGCAGAAGC TCATTGTGAG GGAAGTAGCC AATGAGGAGA AAGCTCTGTT TCTGATCACA 
GCAGGCATCG AGAAGCCCGA GATGGTAGAA GTGTACACAA GCTCCAAAGA GGAGAGAAAC 
ACATGGATGC AGCTCATTCA GGAGGCCATG CAGTCTATAC TGTCCATTTC CTCCAGGGAG 
AGAGATGATG ATGAAGGTGT GCCAAGTGAA AACGAAGAGG ACAGGAGACT TCAGGAAATC 
AAGGTCAAGG AGTTCAGAGA TCAGCTGCAC AAAAGAGACG AGCAGATCTT GACGTTACTG 
GAGGAGAAGG TGAAGTTGTT CAGGGACATG TGTGACTGTG GTGCACCGGA CGAGACGGGG 
CTTCGTAATC GAATGCTCTT CAGGGCCACG ACGGATGATG TCACCAGAGG AGAGCCAATC 
ATGAAGGAGG CTCTGAAGGA AGTTGAAACG CTGCAGGTTC TGGTTAATGA CAGTCTTGGC 
GGTGCGGTTC AGGAAGGTGT GTGCGCGGCT GGACATGTGA CTTTGCCACG ACGAGCGGAA 
ACATTTGGAG GTTTCGACAG TCACCAGATG AACATCTCTA AGAGCAAAGA TGGAGACAGA 
GAGGAGCTGG AGGAGTCTGC AGAGCTCCGC AGGACTGAGT CAGACAGTGT GTTGAAGAAG 
ACGAGTAAGA CCAACCCTTT ACTGCTGGTC AGGAGAAACA ATGAGCAGGT TCTTCAGAGC 
GTTGCTCATC TTCATGACCT GCTGAACACA TTACAAGCTG TGGTGGTCCA GCAGGACACC 
TTCATCGAAG ACCAGCGTCA CGCTCATCCC TCCTCACGCC ACCCATCCAT CTCCTCCTCT 
ACCTCCTCTT TATCATCCAC CTCATCCCGG CCGAGCTCTC TAATCGAGCA GGAGAAACAG 
CGCAGCCTGG AGAAGCAGCG GCAGGAGGTG GCCAGCCTCC AAAAACAGCA GGCGGCCCAC 
GCCGAGGAGA GGCGGCGCAG GGAAAAGCAG TGGGAGACCC GAGAGAAAGA GCTAACCCAG 
AGGGAGATGC AGATCCAGAG CCAAGAAGAG GAGGCGCAGA GGAGAGCCAG ACAGCTGGAG 
GAGGAGAAAC AGGAGCTGCA GAGTAAGAAA GAGGAGTATC AGAGAGACCT GGCACGACTG 
CGGGACAGCC AGAAGAGACT GGAGAGAGAG AGAGAGCAGG TGCAGAGAGA GGCAGATGAG 
CTTAAACTGA CTGAGGAAGT GACAAAGAAC CGGTCTCCGT CAACCACGTC TGAAGACTCC 
ACGAAGTTCC AGAGCACCGG TTCATTAGAC CGTGACCCAT CAGAGATGGA GCTCTCCTCC 
TCCCCGTCAG CCAGAGACTT CCTGTCCCGC ATGGACTCCA AACGCAAAGG GAAAAACCTC 
AATCCATTCT CATCCAACTC AAGCCAGAAG AGCCAGAACA GCGAGGCTCA GAGCCAGATG 
CCCAGCCGTC TGCTCCAGCT GGCCAAACCC AAGGAGAAGA AGGACAAGAA GAAGAAGAAG 
GGGAAAGGCC AGCAGAGCCA GACTGCAGAC TCTCAGAGCA CTGTTGTTCC AGGCGCTACC 
CCAGACGGAG AGATCTTTTT CTGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

wu:fi15d04 ( XM_009297843.2 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_009296118.1 Danio rerio wu:fi15d04 (wu:fi15d04), transcript variant X10, mRNA.

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CloneID	ODa39937
Clone ID Related Accession (Same CDS sequence)	XM_009297843.2 , XM_009297843.3
Accession Version	XM_009297843.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4428bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-23
Organism	Danio rerio(Zebrafish)
Product	A-kinase anchor protein 13 isoform X10
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007136.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_009297843.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTACGAGC GACACCGGCG AAGATACAGC CTGTGCGCGA AGAGCGAGAG ATCGGTGGAT 
GTGGTTTTGA TCAAGATCCA CAGAGAGACC GAAACATTTT ATTCAGTTGA TGCCAGTCAA 
GACTCTGTAC TCCAGGAGAA GAAATCTTCT TCAGTGAGCT CAGAAATCCC CTCTTTGACC 
CCCTCACCTG AGAGCATACG TCACAAAGAG TCGCCAAGCA CTATGCGAGG GTCCGCCAGC 
GATGGAGACT CTCTGTTTAG TCAGGATCTG GGCGAGGACA GCGTTTTTAA TAATAAGGCT 
GAGGACAGCA TTACCATCAC CAGTGGCGTG TCCATGTCCT ATTCGTCCTC CACAGATGAC 
TCTTCCAGCT TGGGAACCAC TTGCGCCACC CCTCAGACTG GCACAGAGGC TCCTGCATCA 
GACCCCTTTC AGGAACAGCC CACATCTACT GGAGCTTCTG GGGAAACAGA GGAGGAAGAG 
AAAAAGGACC GTCTCACTGA GGTTCCGGAG CGATCGGCCA TTTTACGGAC CACCATACGA 
TCACTGTCCC CTTTTCGAAG ACACAGTTGG GGGCCGGGCA AAAATTTGGC AGGGGAGACA 
GAGATGAGTC AGCGCAGTGC GGCACAAGGT GAAGTGGAGC GCAGATCTGT TGGTCATAGG 
AGAAGCATGA GCTGGTGTCC TTCTGTTGTC CTGCGCTCTG AAAATGATGA AATGAATGAA 
AGAAGTTACA GTCTGGAAGG ACTTGCACCA GAGAAGGAGG GAAGGAAAAG TCTGGTTCAG 
CAGCGTGTCG GAGAGTCCAG CCAGGAGCCC AAATGTCCAT CAAAGGTGGA AAAAGAAGAC 
AGAGGCTCGC TTGTGTCTCT GACTGAAGAA GAGCAGGAGT CAGATCTAGG CGACTGCAGC 
AGTTTGGGCA GTCAGAAGTC CATCCAGTCG GGTCGGAGAT GTGTCCCACA TTCACAACCC 
ATCCTCACCA AATCTGTGTC CATGGTGGCC ATCAGTCAGA AGGACCTGGA CGCAATGGGA 
CGGCTGCGAC CCAAGCGACG AATCTCCTTC TCCTTCAGCA TCTCGCCGAT GCTCCCCAAA 
TCTAAAACTC TCTTTTCTCT TGGCTCTTCA TCCAGTGATG AGGAGGAGGC AGTCAGATTG 
AGTTCATTCA CTGGAACATC AGGATCATTA GAGTACAGTA TATCAGAGGA GGATCCTGGG 
CCATTACGGA GTGACAGTGA AGGCAAAATT GGAGGAACCA AAGTGAGCAG GACATTCAGT 
TACCTCAAGA GCAAGATGAG CAAGAAGAAC AAGCAGGAGA AGGAGAAAGA GAAGAATAAG 
GAGCGTGAGC GAGATGCCAA AGACAAGGAG AGAAGAGCCT CAAACGGACA TCTCTTCACT 
ACGGTCATGA CTGTACCCAC AATCCCCTGC CACCAGTGCA ACAAACTCAT CAACACTAAA 
GACTCTATCC TCTGCACCAA TTGCAATGCA CAAGTCCACA AAGGCTGCAA AGAGAGTCTT 
CCTGTCTGCG CTAAAGTCAA GATGAAGCAG CAAAAACAGC AGCAGACTGT TACAGATTCT 
GCCTCGACGC CTAGTGTCAC CCTCAGAAGT AAAACTCTTC CAGCAAGGGA TCGTCCGTGG 
TCCACAATAT CCATTCCCGA TGACCAGCCG GCTCCCCTCG CTCCTCCTCG GAAAAGCCCC 
AGCAGCATCA TGTCTTTCAA CAGCAACCCA CTGTCCAAGA GCATGTCCAT TAATAACATA 
GCAGGACAAA TGGACGACAC TCCTCTTAAG AAGTTTCTGT CCCAGTCAAC GGACTCTCTT 
CACAAGACCA GTAAAGTCAC GGAGTCTACA GAATCTCTTA CTGATGAGGG TACAGAGATG 
ATGGACAGCC AGCTGATGGG AGAATTTGAA GCTGACGCCA AAGAGCTGGA GGCTGATTCT 
TATAGCTTCA CGGTAGACAA GAAATTTCTT AAGCAGCTTA AAAAGGATGT TATCAAAAGG 
CAAGACGTCA TCTATGAGCT CATCCAGACA GAAATGCACC ATGTAAGGAC CTTAAAGATC 
ATGGCTGATG TTTACAGCAA AGGACTACTG AGAGAAGTTC AACTAGAAGC TCAGACGGTG 
GAGAAGATGT TCCCCATGTT GGACGATGTT CTGGATTTGC ACACACAGTT CTTTTCACAC 
CTGCTTGAAA GAAAGAAGGA ATCAGGGAGC CAAGAAGAGG GCGGCTTTGT CATCCAAAGG 
ATTGGCGATG TGCTGGTTTC TCAGTTCTCA GGTTCCAGTG CTGAGAGAAT GAAGAAGGTC 
TATGGGAAGT TCTGCGGCAG GCACAATGAA GCTGTGAACT TCTACAAGGA GCTTCACACC 
AAAGACAAAC GCTTTCAAGC TTTCATCAAG AAAAAGATGA GCAGCCCTGT TGTCCGACGG 
CTAAGCATTC CTGAGTGTAT ATTGTTAGTC ACACAACGAA TCACAAAATA TCCCGTGTTA 
CTACAGAGAA TACTGCAGCA CACGAAAGAA AACGAGGAGG ACCATGCAGA CGTTAGTCAA 
GGTCTGAAGC TGGTGAAGGA GGTGATTGCT GCCGTGGACA ATAAAGTGAA TGAGCATGAG 
AAGAAGAAGA GGCTAAAGGA AGTGTACAGC CGGACGGACA ACAAGTCCAT CATGAGGATG 
AAGAGCGGGC AGATGTTTGC CAGAGAGGAT CTGTTACGTG GGCGGAAGTT AATTCACGAC 
GGGCCGCTGC AGCTCAAGAA CTCAGCTGGC AGACTCAAGG ATGTCCAGGC TCTGCTGCTG 
AGGGACGTGA TTGTGTTCCT GCAGGAGAAA GATCAGAAGT ACATTTTCGC CTCTTTGGAC 
CAGCGCTCCA CCGTCATCTC CCTGCAGAAG CTCATTGTGA GGGAAGTAGC CAATGAGGAG 
AAAGCTCTGT TTCTGATCAC AGCAGGCATC GAGAAGCCCG AGATGGTAGA AGTGTACACA 
AGCTCCAAAG AGGAGAGAAA CACATGGATG CAGCTCATTC AGGAGGCCAT GCAGTCTATA 
CTGTCCATTT CCTCCAGGGA GAGAGATGAT GATGAAGGTG TGCCAAGTGA AAACGAAGAG 
GACAGGAGAC TTCAGGAAAT CAAGGTCAAG GAGTTCAGAG ATCAGCTGCA CAAAAGAGAC 
GAGCAGATCT TGACGTTACT GGAGGAGAAG GTGAAGTTGT TCAGGGACAT GTGTGACTGT 
GGTGCACCGG ACGAGACGGG GCTTCGTAAT CGAATGCTCT TCAGGGCCAC GACGGATGAT 
GTCACCAGAG GAGAGCCAAT CATGAAGGAG GCTCTGAAGG AAGTTGAAAC GCTGCAGGTT 
CTGGTTAATG ACAGTCTTGG CGGTGCGGTT CAGGAAGGTG TGTGCGCGGC TGGACATGTG 
ACTTTGCCAC GACGAGCGGA AACATTTGGA GGTTTCGACA GTCACCAGAT GAACATCTCT 
AAGAGCAAAG ATGGAGACAG AGAGGAGCTG GAGGAGTCTG CAGAGCTCCG CAGGACTGAG 
TCAGACAGTG TGTTGAAGAA GACGAGTAAG ACCAACCCTT TACTGCTGGT CAGGAGAAAC 
AATGAGCAGG TTCTTCAGAG CGTTGCTCAT CTTCATGACC TGCTGAACAC ATTACAAGCT 
GTGGTGGTCC AGCAGGACAC CTTCATCGAA GACCAGCGTC ACGCTCATCC CTCCTCACGC 
CACCCATCCA TCTCCTCCTC TACCTCCTCT TTATCATCCA CCTCATCCCG GCCGAGCTCT 
CTAATCGAGC AGGAGAAACA GCGCAGCCTG GAGAAGCAGC GGCAGGAGGT GGCCAGCCTC 
CAAAAACAGC AGGCGGCCCA CGCCGAGGAG AGGCGGCGCA GGGAAAAGCA GTGGGAGACC 
CGAGAGAAAG AGCTAACCCA GAGGGAGATG CAGATCCAGA GCCAAGAAGA GGAGGCGCAG 
AGGAGAGCCA GACAGCTGGA GGAGGAGAAA CAGGAGCTGC AGAGTAAGAA AGAGGAGTAT 
CAGAGAGACC TGGCACGACT GCGGGACAGC CAGAAGAGAC TGGAGAGAGA GAGAGAGCAG 
GTGCAGAGAG AGGCAGATGA GCTTAAACTG ACTGAGGAAG TGACAAAGAA CCGGTCTCCG 
TCAACCACGT CTGAAGACTC CACGAAGTTC CAGAGCACCG GTTCATTAGA CCGTGACCCA 
TCAGAGATGG AGCTCTCCTC CTCCCCGTCA GCCAGAGACT TCCTGTCCCG CATGGACTCC 
AAACGCAAAG GGAAAAACCT CAATCCATTC TCATCCAACT CAAGCCAGAA GAGCCAGAAC 
AGCGAGGCTC AGAGCCAGAT GCCCAGCCGT CTGCTCCAGC TGGCCAAACC CAAGGAGAAG 
AAGGACAAGA AGAAGAAGAA GGGGAAAGGC CAGCAGAGCC AGACTGCAGA CTCTCAGAGC 
ACTGTTGTTC CAGGCGCTAC CCCAGACGGA GAGATCTTTT TCTGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_009296118.1
CDS	142..4569
Translation	MYERHRRRYSLCAKSERSVDVVLIKIHRETETFYSVDASQDSVLQEKKSSSVSSEIPSLTPSPESIRHKESPSTMRGSASDGDSLFSQDLGEDSVFNNKAEDSITITSGVSMSYSSSTDDSSSLGTTCATPQTGTEAPASDPFQEQPTSTGASGETEEEEKKDRLTEVPERSAILRTTIRSLSPFRRHSWGPGKNLAGETEMSQRSAAQGEVERRSVGHRRSMSWCPSVVLRSENDEMNERSYSLEGLAPEKEGRKSLVQQRVGESSQEPKCPSKVEKEDRGSLVSLTEEEQESDLGDCSSLGSQKSIQSGRRCVPHSQPILTKSVSMVAISQKDLDAMGRLRPKRRISFSFSISPMLPKSKTLFSLGSSSSDEEEAVRLSSFTGTSGSLEYSISEEDPGPLRSDSEGKIGGTKVSRTFSYLKSKMSKKNKQEKEKEKNKERERDAKDKERRASNGHLFTTVMTVPTIPCHQCNKLINTKDSILCTNCNAQVHKGCKESLPVCAKVKMKQQKQQQTVTDSASTPSVTLRSKTLPARDRPWSTISIPDDQPAPLAPPRKSPSSIMSFNSNPLSKSMSINNIAGQMDDTPLKKFLSQSTDSLHKTSKVTESTESLTDEGTEMMDSQLMGEFEADAKELEADSYSFTVDKKFLKQLKKDVIKRQDVIYELIQTEMHHVRTLKIMADVYSKGLLREVQLEAQTVEKMFPMLDDVLDLHTQFFSHLLERKKESGSQEEGGFVIQRIGDVLVSQFSGSSAERMKKVYGKFCGRHNEAVNFYKELHTKDKRFQAFIKKKMSSPVVRRLSIPECILLVTQRITKYPVLLQRILQHTKENEEDHADVSQGLKLVKEVIAAVDNKVNEHEKKKRLKEVYSRTDNKSIMRMKSGQMFAREDLLRGRKLIHDGPLQLKNSAGRLKDVQALLLRDVIVFLQEKDQKYIFASLDQRSTVISLQKLIVREVANEEKALFLITAGIEKPEMVEVYTSSKEERNTWMQLIQEAMQSILSISSRERDDDEGVPSENEEDRRLQEIKVKEFRDQLHKRDEQILTLLEEKVKLFRDMCDCGAPDETGLRNRMLFRATTDDVTRGEPIMKEALKEVETLQVLVNDSLGGAVQEGVCAAGHVTLPRRAETFGGFDSHQMNISKSKDGDREELEESAELRRTESDSVLKKTSKTNPLLLVRRNNEQVLQSVAHLHDLLNTLQAVVVQQDTFIEDQRHAHPSSRHPSISSSTSSLSSTSSRPSSLIEQEKQRSLEKQRQEVASLQKQQAAHAEERRRREKQWETREKELTQREMQIQSQEEEAQRRARQLEEEKQELQSKKEEYQRDLARLRDSQKRLEREREQVQREADELKLTEEVTKNRSPSTTSEDSTKFQSTGSLDRDPSEMELSSSPSARDFLSRMDSKRKGKNLNPFSSNSSQKSQNSEAQSQMPSRLLQLAKPKEKKDKKKKKGKGQQSQTADSQSTVVPGATPDGEIFFC

Target ORF information:

RefSeq Version	XM_009297843.2
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio wu:fi15d04 (wu:fi15d04), transcript variant X10, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_009297843.2

ORF Insert Sequence:

ATGTACGAGC GACACCGGCG AAGATACAGC CTGTGCGCGA AGAGCGAGAG ATCGGTGGAT 
GTGGTTTTGA TCAAGATCCA CAGAGAGACC GAAACATTTT ATTCAGTTGA TGCCAGTCAA 
GACTCTGTAC TCCAGGAGAA GAAATCTTCT TCAGTGAGCT CAGAAATCCC CTCTTTGACC 
CCCTCACCTG AGAGCATACG TCACAAAGAG TCGCCAAGCA CTATGCGAGG GTCCGCCAGC 
GATGGAGACT CTCTGTTTAG TCAGGATCTG GGCGAGGACA GCGTTTTTAA TAATAAGGCT 
GAGGACAGCA TTACCATCAC CAGTGGCGTG TCCATGTCCT ATTCGTCCTC CACAGATGAC 
TCTTCCAGCT TGGGAACCAC TTGCGCCACC CCTCAGACTG GCACAGAGGC TCCTGCATCA 
GACCCCTTTC AGGAACAGCC CACATCTACT GGAGCTTCTG GGGAAACAGA GGAGGAAGAG 
AAAAAGGACC GTCTCACTGA GGTTCCGGAG CGATCGGCCA TTTTACGGAC CACCATACGA 
TCACTGTCCC CTTTTCGAAG ACACAGTTGG GGGCCGGGCA AAAATTTGGC AGGGGAGACA 
GAGATGAGTC AGCGCAGTGC GGCACAAGGT GAAGTGGAGC GCAGATCTGT TGGTCATAGG 
AGAAGCATGA GCTGGTGTCC TTCTGTTGTC CTGCGCTCTG AAAATGATGA AATGAATGAA 
AGAAGTTACA GTCTGGAAGG ACTTGCACCA GAGAAGGAGG GAAGGAAAAG TCTGGTTCAG 
CAGCGTGTCG GAGAGTCCAG CCAGGAGCCC AAATGTCCAT CAAAGGTGGA AAAAGAAGAC 
AGAGGCTCGC TTGTGTCTCT GACTGAAGAA GAGCAGGAGT CAGATCTAGG CGACTGCAGC 
AGTTTGGGCA GTCAGAAGTC CATCCAGTCG GGTCGGAGAT GTGTCCCACA TTCACAACCC 
ATCCTCACCA AATCTGTGTC CATGGTGGCC ATCAGTCAGA AGGACCTGGA CGCAATGGGA 
CGGCTGCGAC CCAAGCGACG AATCTCCTTC TCCTTCAGCA TCTCGCCGAT GCTCCCCAAA 
TCTAAAACTC TCTTTTCTCT TGGCTCTTCA TCCAGTGATG AGGAGGAGGC AGTCAGATTG 
AGTTCATTCA CTGGAACATC AGGATCATTA GAGTACAGTA TATCAGAGGA GGATCCTGGG 
CCATTACGGA GTGACAGTGA AGGCAAAATT GGAGGAACCA AAGTGAGCAG GACATTCAGT 
TACCTCAAGA GCAAGATGAG CAAGAAGAAC AAGCAGGAGA AGGAGAAAGA GAAGAATAAG 
GAGCGTGAGC GAGATGCCAA AGACAAGGAG AGAAGAGCCT CAAACGGACA TCTCTTCACT 
ACGGTCATGA CTGTACCCAC AATCCCCTGC CACCAGTGCA ACAAACTCAT CAACACTAAA 
GACTCTATCC TCTGCACCAA TTGCAATGCA CAAGTCCACA AAGGCTGCAA AGAGAGTCTT 
CCTGTCTGCG CTAAAGTCAA GATGAAGCAG CAAAAACAGC AGCAGACTGT TACAGATTCT 
GCCTCGACGC CTAGTGTCAC CCTCAGAAGT AAAACTCTTC CAGCAAGGGA TCGTCCGTGG 
TCCACAATAT CCATTCCCGA TGACCAGCCG GCTCCCCTCG CTCCTCCTCG GAAAAGCCCC 
AGCAGCATCA TGTCTTTCAA CAGCAACCCA CTGTCCAAGA GCATGTCCAT TAATAACATA 
GCAGGACAAA TGGACGACAC TCCTCTTAAG AAGTTTCTGT CCCAGTCAAC GGACTCTCTT 
CACAAGACCA GTAAAGTCAC GGAGTCTACA GAATCTCTTA CTGATGAGGG TACAGAGATG 
ATGGACAGCC AGCTGATGGG AGAATTTGAA GCTGACGCCA AAGAGCTGGA GGCTGATTCT 
TATAGCTTCA CGGTAGACAA GAAATTTCTT AAGCAGCTTA AAAAGGATGT TATCAAAAGG 
CAAGACGTCA TCTATGAGCT CATCCAGACA GAAATGCACC ATGTAAGGAC CTTAAAGATC 
ATGGCTGATG TTTACAGCAA AGGACTACTG AGAGAAGTTC AACTAGAAGC TCAGACGGTG 
GAGAAGATGT TCCCCATGTT GGACGATGTT CTGGATTTGC ACACACAGTT CTTTTCACAC 
CTGCTTGAAA GAAAGAAGGA ATCAGGGAGC CAAGAAGAGG GCGGCTTTGT CATCCAAAGG 
ATTGGCGATG TGCTGGTTTC TCAGTTCTCA GGTTCCAGTG CTGAGAGAAT GAAGAAGGTC 
TATGGGAAGT TCTGCGGCAG GCACAATGAA GCTGTGAACT TCTACAAGGA GCTTCACACC 
AAAGACAAAC GCTTTCAAGC TTTCATCAAG AAAAAGATGA GCAGCCCTGT TGTCCGACGG 
CTAAGCATTC CTGAGTGTAT ATTGTTAGTC ACACAACGAA TCACAAAATA TCCCGTGTTA 
CTACAGAGAA TACTGCAGCA CACGAAAGAA AACGAGGAGG ACCATGCAGA CGTTAGTCAA 
GGTCTGAAGC TGGTGAAGGA GGTGATTGCT GCCGTGGACA ATAAAGTGAA TGAGCATGAG 
AAGAAGAAGA GGCTAAAGGA AGTGTACAGC CGGACGGACA ACAAGTCCAT CATGAGGATG 
AAGAGCGGGC AGATGTTTGC CAGAGAGGAT CTGTTACGTG GGCGGAAGTT AATTCACGAC 
GGGCCGCTGC AGCTCAAGAA CTCAGCTGGC AGACTCAAGG ATGTCCAGGC TCTGCTGCTG 
AGGGACGTGA TTGTGTTCCT GCAGGAGAAA GATCAGAAGT ACATTTTCGC CTCTTTGGAC 
CAGCGCTCCA CCGTCATCTC CCTGCAGAAG CTCATTGTGA GGGAAGTAGC CAATGAGGAG 
AAAGCTCTGT TTCTGATCAC AGCAGGCATC GAGAAGCCCG AGATGGTAGA AGTGTACACA 
AGCTCCAAAG AGGAGAGAAA CACATGGATG CAGCTCATTC AGGAGGCCAT GCAGTCTATA 
CTGTCCATTT CCTCCAGGGA GAGAGATGAT GATGAAGGTG TGCCAAGTGA AAACGAAGAG 
GACAGGAGAC TTCAGGAAAT CAAGGTCAAG GAGTTCAGAG ATCAGCTGCA CAAAAGAGAC 
GAGCAGATCT TGACGTTACT GGAGGAGAAG GTGAAGTTGT TCAGGGACAT GTGTGACTGT 
GGTGCACCGG ACGAGACGGG GCTTCGTAAT CGAATGCTCT TCAGGGCCAC GACGGATGAT 
GTCACCAGAG GAGAGCCAAT CATGAAGGAG GCTCTGAAGG AAGTTGAAAC GCTGCAGGTT 
CTGGTTAATG ACAGTCTTGG CGGTGCGGTT CAGGAAGGTG TGTGCGCGGC TGGACATGTG 
ACTTTGCCAC GACGAGCGGA AACATTTGGA GGTTTCGACA GTCACCAGAT GAACATCTCT 
AAGAGCAAAG ATGGAGACAG AGAGGAGCTG GAGGAGTCTG CAGAGCTCCG CAGGACTGAG 
TCAGACAGTG TGTTGAAGAA GACGAGTAAG ACCAACCCTT TACTGCTGGT CAGGAGAAAC 
AATGAGCAGG TTCTTCAGAG CGTTGCTCAT CTTCATGACC TGCTGAACAC ATTACAAGCT 
GTGGTGGTCC AGCAGGACAC CTTCATCGAA GACCAGCGTC ACGCTCATCC CTCCTCACGC 
CACCCATCCA TCTCCTCCTC TACCTCCTCT TTATCATCCA CCTCATCCCG GCCGAGCTCT 
CTAATCGAGC AGGAGAAACA GCGCAGCCTG GAGAAGCAGC GGCAGGAGGT GGCCAGCCTC 
CAAAAACAGC AGGCGGCCCA CGCCGAGGAG AGGCGGCGCA GGGAAAAGCA GTGGGAGACC 
CGAGAGAAAG AGCTAACCCA GAGGGAGATG CAGATCCAGA GCCAAGAAGA GGAGGCGCAG 
AGGAGAGCCA GACAGCTGGA GGAGGAGAAA CAGGAGCTGC AGAGTAAGAA AGAGGAGTAT 
CAGAGAGACC TGGCACGACT GCGGGACAGC CAGAAGAGAC TGGAGAGAGA GAGAGAGCAG 
GTGCAGAGAG AGGCAGATGA GCTTAAACTG ACTGAGGAAG TGACAAAGAA CCGGTCTCCG 
TCAACCACGT CTGAAGACTC CACGAAGTTC CAGAGCACCG GTTCATTAGA CCGTGACCCA 
TCAGAGATGG AGCTCTCCTC CTCCCCGTCA GCCAGAGACT TCCTGTCCCG CATGGACTCC 
AAACGCAAAG GGAAAAACCT CAATCCATTC TCATCCAACT CAAGCCAGAA GAGCCAGAAC 
AGCGAGGCTC AGAGCCAGAT GCCCAGCCGT CTGCTCCAGC TGGCCAAACC CAAGGAGAAG 
AAGGACAAGA AGAAGAAGAA GGGGAAAGGC CAGCAGAGCC AGACTGCAGA CTCTCAGAGC 
ACTGTTGTTC CAGGCGCTAC CCCAGACGGA GAGATCTTTT TCTGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

wu:fi15d04 ( XM_009297843.3 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_009296118.1 Danio rerio wu:fi15d04 (wu:fi15d04), transcript variant X10, mRNA.

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CloneID	ODa39937
Clone ID Related Accession (Same CDS sequence)	XM_009297843.2 , XM_009297843.3
Accession Version	XM_009297843.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4428bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	A-kinase anchor protein 13 isoform X10
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007136.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_009297843.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTACGAGC GACACCGGCG AAGATACAGC CTGTGCGCGA AGAGCGAGAG ATCGGTGGAT 
GTGGTTTTGA TCAAGATCCA CAGAGAGACC GAAACATTTT ATTCAGTTGA TGCCAGTCAA 
GACTCTGTAC TCCAGGAGAA GAAATCTTCT TCAGTGAGCT CAGAAATCCC CTCTTTGACC 
CCCTCACCTG AGAGCATACG TCACAAAGAG TCGCCAAGCA CTATGCGAGG GTCCGCCAGC 
GATGGAGACT CTCTGTTTAG TCAGGATCTG GGCGAGGACA GCGTTTTTAA TAATAAGGCT 
GAGGACAGCA TTACCATCAC CAGTGGCGTG TCCATGTCCT ATTCGTCCTC CACAGATGAC 
TCTTCCAGCT TGGGAACCAC TTGCGCCACC CCTCAGACTG GCACAGAGGC TCCTGCATCA 
GACCCCTTTC AGGAACAGCC CACATCTACT GGAGCTTCTG GGGAAACAGA GGAGGAAGAG 
AAAAAGGACC GTCTCACTGA GGTTCCGGAG CGATCGGCCA TTTTACGGAC CACCATACGA 
TCACTGTCCC CTTTTCGAAG ACACAGTTGG GGGCCGGGCA AAAATTTGGC AGGGGAGACA 
GAGATGAGTC AGCGCAGTGC GGCACAAGGT GAAGTGGAGC GCAGATCTGT TGGTCATAGG 
AGAAGCATGA GCTGGTGTCC TTCTGTTGTC CTGCGCTCTG AAAATGATGA AATGAATGAA 
AGAAGTTACA GTCTGGAAGG ACTTGCACCA GAGAAGGAGG GAAGGAAAAG TCTGGTTCAG 
CAGCGTGTCG GAGAGTCCAG CCAGGAGCCC AAATGTCCAT CAAAGGTGGA AAAAGAAGAC 
AGAGGCTCGC TTGTGTCTCT GACTGAAGAA GAGCAGGAGT CAGATCTAGG CGACTGCAGC 
AGTTTGGGCA GTCAGAAGTC CATCCAGTCG GGTCGGAGAT GTGTCCCACA TTCACAACCC 
ATCCTCACCA AATCTGTGTC CATGGTGGCC ATCAGTCAGA AGGACCTGGA CGCAATGGGA 
CGGCTGCGAC CCAAGCGACG AATCTCCTTC TCCTTCAGCA TCTCGCCGAT GCTCCCCAAA 
TCTAAAACTC TCTTTTCTCT TGGCTCTTCA TCCAGTGATG AGGAGGAGGC AGTCAGATTG 
AGTTCATTCA CTGGAACATC AGGATCATTA GAGTACAGTA TATCAGAGGA GGATCCTGGG 
CCATTACGGA GTGACAGTGA AGGCAAAATT GGAGGAACCA AAGTGAGCAG GACATTCAGT 
TACCTCAAGA GCAAGATGAG CAAGAAGAAC AAGCAGGAGA AGGAGAAAGA GAAGAATAAG 
GAGCGTGAGC GAGATGCCAA AGACAAGGAG AGAAGAGCCT CAAACGGACA TCTCTTCACT 
ACGGTCATGA CTGTACCCAC AATCCCCTGC CACCAGTGCA ACAAACTCAT CAACACTAAA 
GACTCTATCC TCTGCACCAA TTGCAATGCA CAAGTCCACA AAGGCTGCAA AGAGAGTCTT 
CCTGTCTGCG CTAAAGTCAA GATGAAGCAG CAAAAACAGC AGCAGACTGT TACAGATTCT 
GCCTCGACGC CTAGTGTCAC CCTCAGAAGT AAAACTCTTC CAGCAAGGGA TCGTCCGTGG 
TCCACAATAT CCATTCCCGA TGACCAGCCG GCTCCCCTCG CTCCTCCTCG GAAAAGCCCC 
AGCAGCATCA TGTCTTTCAA CAGCAACCCA CTGTCCAAGA GCATGTCCAT TAATAACATA 
GCAGGACAAA TGGACGACAC TCCTCTTAAG AAGTTTCTGT CCCAGTCAAC GGACTCTCTT 
CACAAGACCA GTAAAGTCAC GGAGTCTACA GAATCTCTTA CTGATGAGGG TACAGAGATG 
ATGGACAGCC AGCTGATGGG AGAATTTGAA GCTGACGCCA AAGAGCTGGA GGCTGATTCT 
TATAGCTTCA CGGTAGACAA GAAATTTCTT AAGCAGCTTA AAAAGGATGT TATCAAAAGG 
CAAGACGTCA TCTATGAGCT CATCCAGACA GAAATGCACC ATGTAAGGAC CTTAAAGATC 
ATGGCTGATG TTTACAGCAA AGGACTACTG AGAGAAGTTC AACTAGAAGC TCAGACGGTG 
GAGAAGATGT TCCCCATGTT GGACGATGTT CTGGATTTGC ACACACAGTT CTTTTCACAC 
CTGCTTGAAA GAAAGAAGGA ATCAGGGAGC CAAGAAGAGG GCGGCTTTGT CATCCAAAGG 
ATTGGCGATG TGCTGGTTTC TCAGTTCTCA GGTTCCAGTG CTGAGAGAAT GAAGAAGGTC 
TATGGGAAGT TCTGCGGCAG GCACAATGAA GCTGTGAACT TCTACAAGGA GCTTCACACC 
AAAGACAAAC GCTTTCAAGC TTTCATCAAG AAAAAGATGA GCAGCCCTGT TGTCCGACGG 
CTAAGCATTC CTGAGTGTAT ATTGTTAGTC ACACAACGAA TCACAAAATA TCCCGTGTTA 
CTACAGAGAA TACTGCAGCA CACGAAAGAA AACGAGGAGG ACCATGCAGA CGTTAGTCAA 
GGTCTGAAGC TGGTGAAGGA GGTGATTGCT GCCGTGGACA ATAAAGTGAA TGAGCATGAG 
AAGAAGAAGA GGCTAAAGGA AGTGTACAGC CGGACGGACA ACAAGTCCAT CATGAGGATG 
AAGAGCGGGC AGATGTTTGC CAGAGAGGAT CTGTTACGTG GGCGGAAGTT AATTCACGAC 
GGGCCGCTGC AGCTCAAGAA CTCAGCTGGC AGACTCAAGG ATGTCCAGGC TCTGCTGCTG 
AGGGACGTGA TTGTGTTCCT GCAGGAGAAA GATCAGAAGT ACATTTTCGC CTCTTTGGAC 
CAGCGCTCCA CCGTCATCTC CCTGCAGAAG CTCATTGTGA GGGAAGTAGC CAATGAGGAG 
AAAGCTCTGT TTCTGATCAC AGCAGGCATC GAGAAGCCCG AGATGGTAGA AGTGTACACA 
AGCTCCAAAG AGGAGAGAAA CACATGGATG CAGCTCATTC AGGAGGCCAT GCAGTCTATA 
CTGTCCATTT CCTCCAGGGA GAGAGATGAT GATGAAGGTG TGCCAAGTGA AAACGAAGAG 
GACAGGAGAC TTCAGGAAAT CAAGGTCAAG GAGTTCAGAG ATCAGCTGCA CAAAAGAGAC 
GAGCAGATCT TGACGTTACT GGAGGAGAAG GTGAAGTTGT TCAGGGACAT GTGTGACTGT 
GGTGCACCGG ACGAGACGGG GCTTCGTAAT CGAATGCTCT TCAGGGCCAC GACGGATGAT 
GTCACCAGAG GAGAGCCAAT CATGAAGGAG GCTCTGAAGG AAGTTGAAAC GCTGCAGGTT 
CTGGTTAATG ACAGTCTTGG CGGTGCGGTT CAGGAAGGTG TGTGCGCGGC TGGACATGTG 
ACTTTGCCAC GACGAGCGGA AACATTTGGA GGTTTCGACA GTCACCAGAT GAACATCTCT 
AAGAGCAAAG ATGGAGACAG AGAGGAGCTG GAGGAGTCTG CAGAGCTCCG CAGGACTGAG 
TCAGACAGTG TGTTGAAGAA GACGAGTAAG ACCAACCCTT TACTGCTGGT CAGGAGAAAC 
AATGAGCAGG TTCTTCAGAG CGTTGCTCAT CTTCATGACC TGCTGAACAC ATTACAAGCT 
GTGGTGGTCC AGCAGGACAC CTTCATCGAA GACCAGCGTC ACGCTCATCC CTCCTCACGC 
CACCCATCCA TCTCCTCCTC TACCTCCTCT TTATCATCCA CCTCATCCCG GCCGAGCTCT 
CTAATCGAGC AGGAGAAACA GCGCAGCCTG GAGAAGCAGC GGCAGGAGGT GGCCAGCCTC 
CAAAAACAGC AGGCGGCCCA CGCCGAGGAG AGGCGGCGCA GGGAAAAGCA GTGGGAGACC 
CGAGAGAAAG AGCTAACCCA GAGGGAGATG CAGATCCAGA GCCAAGAAGA GGAGGCGCAG 
AGGAGAGCCA GACAGCTGGA GGAGGAGAAA CAGGAGCTGC AGAGTAAGAA AGAGGAGTAT 
CAGAGAGACC TGGCACGACT GCGGGACAGC CAGAAGAGAC TGGAGAGAGA GAGAGAGCAG 
GTGCAGAGAG AGGCAGATGA GCTTAAACTG ACTGAGGAAG TGACAAAGAA CCGGTCTCCG 
TCAACCACGT CTGAAGACTC CACGAAGTTC CAGAGCACCG GTTCATTAGA CCGTGACCCA 
TCAGAGATGG AGCTCTCCTC CTCCCCGTCA GCCAGAGACT TCCTGTCCCG CATGGACTCC 
AAACGCAAAG GGAAAAACCT CAATCCATTC TCATCCAACT CAAGCCAGAA GAGCCAGAAC 
AGCGAGGCTC AGAGCCAGAT GCCCAGCCGT CTGCTCCAGC TGGCCAAACC CAAGGAGAAG 
AAGGACAAGA AGAAGAAGAA GGGGAAAGGC CAGCAGAGCC AGACTGCAGA CTCTCAGAGC 
ACTGTTGTTC CAGGCGCTAC CCCAGACGGA GAGATCTTTT TCTGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_009296118.1
CDS	150..4577
Translation	MYERHRRRYSLCAKSERSVDVVLIKIHRETETFYSVDASQDSVLQEKKSSSVSSEIPSLTPSPESIRHKESPSTMRGSASDGDSLFSQDLGEDSVFNNKAEDSITITSGVSMSYSSSTDDSSSLGTTCATPQTGTEAPASDPFQEQPTSTGASGETEEEEKKDRLTEVPERSAILRTTIRSLSPFRRHSWGPGKNLAGETEMSQRSAAQGEVERRSVGHRRSMSWCPSVVLRSENDEMNERSYSLEGLAPEKEGRKSLVQQRVGESSQEPKCPSKVEKEDRGSLVSLTEEEQESDLGDCSSLGSQKSIQSGRRCVPHSQPILTKSVSMVAISQKDLDAMGRLRPKRRISFSFSISPMLPKSKTLFSLGSSSSDEEEAVRLSSFTGTSGSLEYSISEEDPGPLRSDSEGKIGGTKVSRTFSYLKSKMSKKNKQEKEKEKNKERERDAKDKERRASNGHLFTTVMTVPTIPCHQCNKLINTKDSILCTNCNAQVHKGCKESLPVCAKVKMKQQKQQQTVTDSASTPSVTLRSKTLPARDRPWSTISIPDDQPAPLAPPRKSPSSIMSFNSNPLSKSMSINNIAGQMDDTPLKKFLSQSTDSLHKTSKVTESTESLTDEGTEMMDSQLMGEFEADAKELEADSYSFTVDKKFLKQLKKDVIKRQDVIYELIQTEMHHVRTLKIMADVYSKGLLREVQLEAQTVEKMFPMLDDVLDLHTQFFSHLLERKKESGSQEEGGFVIQRIGDVLVSQFSGSSAERMKKVYGKFCGRHNEAVNFYKELHTKDKRFQAFIKKKMSSPVVRRLSIPECILLVTQRITKYPVLLQRILQHTKENEEDHADVSQGLKLVKEVIAAVDNKVNEHEKKKRLKEVYSRTDNKSIMRMKSGQMFAREDLLRGRKLIHDGPLQLKNSAGRLKDVQALLLRDVIVFLQEKDQKYIFASLDQRSTVISLQKLIVREVANEEKALFLITAGIEKPEMVEVYTSSKEERNTWMQLIQEAMQSILSISSRERDDDEGVPSENEEDRRLQEIKVKEFRDQLHKRDEQILTLLEEKVKLFRDMCDCGAPDETGLRNRMLFRATTDDVTRGEPIMKEALKEVETLQVLVNDSLGGAVQEGVCAAGHVTLPRRAETFGGFDSHQMNISKSKDGDREELEESAELRRTESDSVLKKTSKTNPLLLVRRNNEQVLQSVAHLHDLLNTLQAVVVQQDTFIEDQRHAHPSSRHPSISSSTSSLSSTSSRPSSLIEQEKQRSLEKQRQEVASLQKQQAAHAEERRRREKQWETREKELTQREMQIQSQEEEAQRRARQLEEEKQELQSKKEEYQRDLARLRDSQKRLEREREQVQREADELKLTEEVTKNRSPSTTSEDSTKFQSTGSLDRDPSEMELSSSPSARDFLSRMDSKRKGKNLNPFSSNSSQKSQNSEAQSQMPSRLLQLAKPKEKKDKKKKKGKGQQSQTADSQSTVVPGATPDGEIFFC

Target ORF information:

RefSeq Version	XM_009297843.3
Organism	Danio rerio(zebrafish)
Definition	Danio rerio wu:fi15d04 (wu:fi15d04), transcript variant X10, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_009297843.3

ORF Insert Sequence:

ATGTACGAGC GACACCGGCG AAGATACAGC CTGTGCGCGA AGAGCGAGAG ATCGGTGGAT 
GTGGTTTTGA TCAAGATCCA CAGAGAGACC GAAACATTTT ATTCAGTTGA TGCCAGTCAA 
GACTCTGTAC TCCAGGAGAA GAAATCTTCT TCAGTGAGCT CAGAAATCCC CTCTTTGACC 
CCCTCACCTG AGAGCATACG TCACAAAGAG TCGCCAAGCA CTATGCGAGG GTCCGCCAGC 
GATGGAGACT CTCTGTTTAG TCAGGATCTG GGCGAGGACA GCGTTTTTAA TAATAAGGCT 
GAGGACAGCA TTACCATCAC CAGTGGCGTG TCCATGTCCT ATTCGTCCTC CACAGATGAC 
TCTTCCAGCT TGGGAACCAC TTGCGCCACC CCTCAGACTG GCACAGAGGC TCCTGCATCA 
GACCCCTTTC AGGAACAGCC CACATCTACT GGAGCTTCTG GGGAAACAGA GGAGGAAGAG 
AAAAAGGACC GTCTCACTGA GGTTCCGGAG CGATCGGCCA TTTTACGGAC CACCATACGA 
TCACTGTCCC CTTTTCGAAG ACACAGTTGG GGGCCGGGCA AAAATTTGGC AGGGGAGACA 
GAGATGAGTC AGCGCAGTGC GGCACAAGGT GAAGTGGAGC GCAGATCTGT TGGTCATAGG 
AGAAGCATGA GCTGGTGTCC TTCTGTTGTC CTGCGCTCTG AAAATGATGA AATGAATGAA 
AGAAGTTACA GTCTGGAAGG ACTTGCACCA GAGAAGGAGG GAAGGAAAAG TCTGGTTCAG 
CAGCGTGTCG GAGAGTCCAG CCAGGAGCCC AAATGTCCAT CAAAGGTGGA AAAAGAAGAC 
AGAGGCTCGC TTGTGTCTCT GACTGAAGAA GAGCAGGAGT CAGATCTAGG CGACTGCAGC 
AGTTTGGGCA GTCAGAAGTC CATCCAGTCG GGTCGGAGAT GTGTCCCACA TTCACAACCC 
ATCCTCACCA AATCTGTGTC CATGGTGGCC ATCAGTCAGA AGGACCTGGA CGCAATGGGA 
CGGCTGCGAC CCAAGCGACG AATCTCCTTC TCCTTCAGCA TCTCGCCGAT GCTCCCCAAA 
TCTAAAACTC TCTTTTCTCT TGGCTCTTCA TCCAGTGATG AGGAGGAGGC AGTCAGATTG 
AGTTCATTCA CTGGAACATC AGGATCATTA GAGTACAGTA TATCAGAGGA GGATCCTGGG 
CCATTACGGA GTGACAGTGA AGGCAAAATT GGAGGAACCA AAGTGAGCAG GACATTCAGT 
TACCTCAAGA GCAAGATGAG CAAGAAGAAC AAGCAGGAGA AGGAGAAAGA GAAGAATAAG 
GAGCGTGAGC GAGATGCCAA AGACAAGGAG AGAAGAGCCT CAAACGGACA TCTCTTCACT 
ACGGTCATGA CTGTACCCAC AATCCCCTGC CACCAGTGCA ACAAACTCAT CAACACTAAA 
GACTCTATCC TCTGCACCAA TTGCAATGCA CAAGTCCACA AAGGCTGCAA AGAGAGTCTT 
CCTGTCTGCG CTAAAGTCAA GATGAAGCAG CAAAAACAGC AGCAGACTGT TACAGATTCT 
GCCTCGACGC CTAGTGTCAC CCTCAGAAGT AAAACTCTTC CAGCAAGGGA TCGTCCGTGG 
TCCACAATAT CCATTCCCGA TGACCAGCCG GCTCCCCTCG CTCCTCCTCG GAAAAGCCCC 
AGCAGCATCA TGTCTTTCAA CAGCAACCCA CTGTCCAAGA GCATGTCCAT TAATAACATA 
GCAGGACAAA TGGACGACAC TCCTCTTAAG AAGTTTCTGT CCCAGTCAAC GGACTCTCTT 
CACAAGACCA GTAAAGTCAC GGAGTCTACA GAATCTCTTA CTGATGAGGG TACAGAGATG 
ATGGACAGCC AGCTGATGGG AGAATTTGAA GCTGACGCCA AAGAGCTGGA GGCTGATTCT 
TATAGCTTCA CGGTAGACAA GAAATTTCTT AAGCAGCTTA AAAAGGATGT TATCAAAAGG 
CAAGACGTCA TCTATGAGCT CATCCAGACA GAAATGCACC ATGTAAGGAC CTTAAAGATC 
ATGGCTGATG TTTACAGCAA AGGACTACTG AGAGAAGTTC AACTAGAAGC TCAGACGGTG 
GAGAAGATGT TCCCCATGTT GGACGATGTT CTGGATTTGC ACACACAGTT CTTTTCACAC 
CTGCTTGAAA GAAAGAAGGA ATCAGGGAGC CAAGAAGAGG GCGGCTTTGT CATCCAAAGG 
ATTGGCGATG TGCTGGTTTC TCAGTTCTCA GGTTCCAGTG CTGAGAGAAT GAAGAAGGTC 
TATGGGAAGT TCTGCGGCAG GCACAATGAA GCTGTGAACT TCTACAAGGA GCTTCACACC 
AAAGACAAAC GCTTTCAAGC TTTCATCAAG AAAAAGATGA GCAGCCCTGT TGTCCGACGG 
CTAAGCATTC CTGAGTGTAT ATTGTTAGTC ACACAACGAA TCACAAAATA TCCCGTGTTA 
CTACAGAGAA TACTGCAGCA CACGAAAGAA AACGAGGAGG ACCATGCAGA CGTTAGTCAA 
GGTCTGAAGC TGGTGAAGGA GGTGATTGCT GCCGTGGACA ATAAAGTGAA TGAGCATGAG 
AAGAAGAAGA GGCTAAAGGA AGTGTACAGC CGGACGGACA ACAAGTCCAT CATGAGGATG 
AAGAGCGGGC AGATGTTTGC CAGAGAGGAT CTGTTACGTG GGCGGAAGTT AATTCACGAC 
GGGCCGCTGC AGCTCAAGAA CTCAGCTGGC AGACTCAAGG ATGTCCAGGC TCTGCTGCTG 
AGGGACGTGA TTGTGTTCCT GCAGGAGAAA GATCAGAAGT ACATTTTCGC CTCTTTGGAC 
CAGCGCTCCA CCGTCATCTC CCTGCAGAAG CTCATTGTGA GGGAAGTAGC CAATGAGGAG 
AAAGCTCTGT TTCTGATCAC AGCAGGCATC GAGAAGCCCG AGATGGTAGA AGTGTACACA 
AGCTCCAAAG AGGAGAGAAA CACATGGATG CAGCTCATTC AGGAGGCCAT GCAGTCTATA 
CTGTCCATTT CCTCCAGGGA GAGAGATGAT GATGAAGGTG TGCCAAGTGA AAACGAAGAG 
GACAGGAGAC TTCAGGAAAT CAAGGTCAAG GAGTTCAGAG ATCAGCTGCA CAAAAGAGAC 
GAGCAGATCT TGACGTTACT GGAGGAGAAG GTGAAGTTGT TCAGGGACAT GTGTGACTGT 
GGTGCACCGG ACGAGACGGG GCTTCGTAAT CGAATGCTCT TCAGGGCCAC GACGGATGAT 
GTCACCAGAG GAGAGCCAAT CATGAAGGAG GCTCTGAAGG AAGTTGAAAC GCTGCAGGTT 
CTGGTTAATG ACAGTCTTGG CGGTGCGGTT CAGGAAGGTG TGTGCGCGGC TGGACATGTG 
ACTTTGCCAC GACGAGCGGA AACATTTGGA GGTTTCGACA GTCACCAGAT GAACATCTCT 
AAGAGCAAAG ATGGAGACAG AGAGGAGCTG GAGGAGTCTG CAGAGCTCCG CAGGACTGAG 
TCAGACAGTG TGTTGAAGAA GACGAGTAAG ACCAACCCTT TACTGCTGGT CAGGAGAAAC 
AATGAGCAGG TTCTTCAGAG CGTTGCTCAT CTTCATGACC TGCTGAACAC ATTACAAGCT 
GTGGTGGTCC AGCAGGACAC CTTCATCGAA GACCAGCGTC ACGCTCATCC CTCCTCACGC 
CACCCATCCA TCTCCTCCTC TACCTCCTCT TTATCATCCA CCTCATCCCG GCCGAGCTCT 
CTAATCGAGC AGGAGAAACA GCGCAGCCTG GAGAAGCAGC GGCAGGAGGT GGCCAGCCTC 
CAAAAACAGC AGGCGGCCCA CGCCGAGGAG AGGCGGCGCA GGGAAAAGCA GTGGGAGACC 
CGAGAGAAAG AGCTAACCCA GAGGGAGATG CAGATCCAGA GCCAAGAAGA GGAGGCGCAG 
AGGAGAGCCA GACAGCTGGA GGAGGAGAAA CAGGAGCTGC AGAGTAAGAA AGAGGAGTAT 
CAGAGAGACC TGGCACGACT GCGGGACAGC CAGAAGAGAC TGGAGAGAGA GAGAGAGCAG 
GTGCAGAGAG AGGCAGATGA GCTTAAACTG ACTGAGGAAG TGACAAAGAA CCGGTCTCCG 
TCAACCACGT CTGAAGACTC CACGAAGTTC CAGAGCACCG GTTCATTAGA CCGTGACCCA 
TCAGAGATGG AGCTCTCCTC CTCCCCGTCA GCCAGAGACT TCCTGTCCCG CATGGACTCC 
AAACGCAAAG GGAAAAACCT CAATCCATTC TCATCCAACT CAAGCCAGAA GAGCCAGAAC 
AGCGAGGCTC AGAGCCAGAT GCCCAGCCGT CTGCTCCAGC TGGCCAAACC CAAGGAGAAG 
AAGGACAAGA AGAAGAAGAA GGGGAAAGGC CAGCAGAGCC AGACTGCAGA CTCTCAGAGC 
ACTGTTGTTC CAGGCGCTAC CCCAGACGGA GAGATCTTTT TCTGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

wu:fi15d04 ( XM_021470630.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_021326305.1 Danio rerio wu:fi15d04 (wu:fi15d04), transcript variant X2, mRNA.

Price & Availability↑

CloneID	ODa141425
Clone ID Related Accession (Same CDS sequence)	XM_021470630.1
Accession Version	XM_021470630.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7659bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	A-kinase anchor protein 13 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007136.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAACTGA ATCCACAGCA AGCCCCATTA TATGGAGAGT GTCTGCTGAC GGTCCAGCTC 
TGTGAGGATG AGTGTGTTGA GGATGAGGAT GATGTGGAGT TTTACCTGCT CTTTGCTGGA 
TCCACACAGA GACATGTGTC CAGCACACTG AAGATCAGCC ATGTCACGCT GCAGGCCGTC 
TGTCCAGCTC ATGATCGCAG TGAAACGGTA CGGGTCACAC TATGCCAGGC TCGTCCCGGC 
GGCTCAGTGG ACCCAGTGTC CGAGGAGTGT TTCCAGTTTG TCCAGGACCT GGCTCTGGAC 
ATGGCCCAGT TCCTGGTAAA TGCGGCGGGG AAAACAGAAG GAGCGCTGCT GCTGGACGAG 
TGTCAGATCC CACTGAAAGA GTGCGAGCGG CTGGACGATA CTCTTGCTCT GGCTCTCCGG 
CATCTGCCTT TGCCTGCTGG ATGGAGCGTT TTGGGCACAG ATTTGGACAC GGGCACTTGC 
ACAGGCTCGG GCATCGAGCC TGGACCACAC GATACACTTT TGCACTTCGC TGCGAAGCGC 
GGACTACGCA AGGTTGCACT CTTTCTGCTC CAGCAACCAG GGGGAAGAGA GGCTCTCCGG 
CTTCCCAACA AACAGGGTCG CACACCGGCC AAGGTCGCCC AGAAGAGGGG ACACTCTCAG 
CTCCACAAAC TGCTCACGGA GGTTGACAAT CTGCCCGAGC TGGAGACGAA AACATCTAAG 
TGGTGTTACC CAGAAGGCAG AGTCCTGCAA CACCACCCCA AGCTAAACAC TTACACCCTT 
ACCCTGGAGG ATGTGCCCAG GAGCTCCCCA CCAAGCCTTC AGTGTGATGT GGAGGAGCTC 
CAGCGTCTCA TTCAGTCACA TCAGAAGGAA GATCCTCCAA CACCGCATAA GTCAGCACTG 
CTGATTCTCA ATAGAGACTC CAGTAATAGC ACAGAGACAG ATGACTGCCT TCCGCCCAAC 
TCAGTGGAAC CTGAGCATCA GGAGCACAAC AAACAAGAAG AAACTTGCAA ATCGCTTTTA 
AACAGCCTTA CCTTTGAGGA TGGATTGTGT GTTCATCAAA ACGGCACAGG AGATCATGTC 
CAGTTGCTGG ATGGGGACGT GGAGCCTGAC CTGCCAGCTG TAGGAGATAC AGGGAATGCA 
TTAGATAAGA CTGACTGTTT GCAGCGGCGG GTAGATGGTG ATTCTGTGGC TTTCAGTGCC 
CCTTCCTGTG GAAACCAGCG GGAGGAAGCC GATATAGAGC TCTGCATAAT CAGTGCCAAG 
CAGGGGGTTG CTTCACAGCC TGATAAAGGG AAGCAAGAAG AGCAAGAGGC TTGCCAGAAG 
AGGTCAGAGA CTGAAAAGGA CTATAACACT GAATCTGGCA ACCTTGGGCT TCAAGACATG 
GGCCACGCAC AATCTCAGGG TCTCCTCCCC ACGGAAACTG GTCAGCCTAC ACAAAGGGAG 
GTGGAGAGTG AGGGGAGTAC AGATGTCTGT TGGCGTGGGG ACTTACCAGA GGAAAGGGAA 
GGGGACATGA GTAGCAACCA GGAAATAGAC TCATATTCTT CAGCACTTTC TTCCCATAAT 
CTAGAGGGAA ACAGCCAGCA CGATGGTGAG ACTGAGCCCC AGTTGCCTAC ACAATGCTCA 
GAGGGAACAG ATGAAGCTAA ATTGACTTTA ATTCAGGTAA ATTCTGAAGT TACTTTGTCT 
ATACGAAGCG AGGATACCGT TATTTCTGAC AGTGTTGACA AGCCACATGG TCTCTTAGAG 
ACGGAGACAG GAAATCTCAG CGAACACACC AAAGGCAACA CTGAGTCAGC TGCTGATTTG 
GTTGCGGATG ACGCTAGTGT AAAGCAGAAC TCTGGATTTA GCGTTTTAGA GGGGGATCTT 
TTGGAATCAT TAAGTCTACG GCCAAACATG GCACCGGGAC CAGTGCATAA GGTTCAGCAA 
GGTCTGTCAC CAGTTCCTGA GACTTCCTCT CATGTAGGTC CAGCTTTGGA CCCCTTTTCT 
GAGACTACAG AAAGTTTTCA ACAGCCGAAT CAATCCACAC AGCTTTTAGA AACTGCATTA 
GAATGTGAGG ATGTTTCATT AAATAACTCA ACCGGTGACA TTAATGCAAT AGCAGGGGTT 
TCTGTTGACA TTTCTGCAGA GGTTTCACTT TGCCAGAATG AAGGGTGTCC TCCTGATGGA 
AGCAAAGAAG AGGTAGCCCC CAGTGAGAGG ATACCAGACC TTAAAGAGCT TACGGAAGAG 
CCGGAATCAG TTGAGGACAT TATTGTCTCA GATGTCTCAG ATTTGGATGA CAAATCTCAA 
CCTTTTGAAG AGTCTTCTTT GCCTATCGAC TCTGAATGTT TTATAAGAGG TGATTTCCAA 
AACGATGAGT CTACAGTACA GTCTTCTGAA TGTACTGGAG GTGACATAAA TGTAGGAACA 
CTCTTAGGAG AAGAAGAGGC AACTGAAAAA ATAGAACAGC CAGACTCTTC ACTTAAACTG 
GTTGAGCTAA TTCCTGAGGA ATATACTGGA GATGCCACAA ACGAAAATAA AAAAATTGAG 
CCAGAGGAAC AATCTATTTT ACTGCCAACT TTGCAAGGGG AATCAGGATT TAAGGATGTT 
GCCACAGAGG AACCTGTACA GGAAAGGGAA ATTGACTCCA CTTCAGAGGA AACAATCAGC 
GATCTCCCTG ATCCATCGGA AATCACAAAC AATGATTGTG CTGAATCCAA TGCACACTGC 
CAGCTCTCAG AATCCTCCAA GGAGAGCGCA AACACATCCT CAGAACCTGC TGAATGTGTG 
AATGACCTAG ACTTGTCTTG TGCTTTACCT CAAACAGAAA TCGAAGGCAG CAGACCTTCT 
GAGGGCCCCT TAATTGATGG AGTAACAATA TGTGCTGAAA TTCCCATTGA TGACAAAGGT 
GTTGACATGG CTACCAATAT GAGTAAGACG ACCATCTCTG AGGATTGTCT GTCCTCTCCT 
ATGGACACCA CCAGCTGTTC AGTTCTGGAG AGCTCCTCCA TGTCTGACCC ACTCAATGCC 
TCATCCTCAA CAGCTGAGCT CACTTCCCCT TTGGATATTA ATAGTGGATT GCATCAGGAT 
TTGGAACACA CTTCCCCAGT GGATATAGAC AATGGTTTGC CTGAGGAACC GCCGCCGTCA 
GACACACCTG ATTGCCTGAA TGGATTACAT CAAGATTCTC CTGATAGTTT GATGGAGCAC 
AGTGGAACTC CTCAGCCATG TTCTGAGGAA CAGTGTGTGG ATCAAATCAC AGATGACAGC 
ACTTCAGCAG AGGATGCAGC GGACGGACAC CAAGACTCCT CAGTAGACAT AGCAAATTCC 
GAAGCGATCC ACAGAGAGAC CGAAACATTT TATTCAGTTG ATGCCAGTCA AGACTCTGTA 
CTCCAGGAGA AGAAATCTTC TTCAGTGAGC TCAGAAATCC CCTCTTTGAC CCCCTCACCT 
GAGAGCATAC GTCACAAAGA GTCGCCAAGC ACTATGCGAG GGTCCGCCAG CGATGGAGAC 
TCTCTGTTTA GTCAGGATCT GGGCGAGGAC AGCGTTTTTA ATAATAAGGC TGAGGACAGC 
ATTACCATCA CCAGTGGCGT GTCCATGTCC TATTCGTCCT CCACAGATGA CTCTTCCAGC 
TTGGGAACCA CTTGCGCCAC CCCTCAGACT GGCACAGAGG CTCCTGCATC AGACCCCTTT 
CAGGAACAGC CCACATCTAC TGGAGCTTCT GGGGAAACAG AGGAGGAAGA GAAAAAGGAC 
CGTCTCACTG AGGTTCCGGA GCGATCGGCC ATTTTACGGA CCACCATACG ATCACTGTCC 
CCTTTTCGAA GACACAGTTG GGGGCCGGGC AAAAATTTGG CAGGGGAGAC AGAGATGAGT 
CAGCGCAGTG CGGCACAAGG TGAAGTGGAG CGCAGATCTG TTGGTCATAG GAGAAGCATG 
AGCTGGTGTC CTTCTGTTGT CCTGCGCTCT GAAAATGATG AAATGAATGA AAGAAGTTAC 
AGTCTGGAAG GACTTGCACC AGAGAAGGAG GGAAGGAAAA GTCTGGTTCA GCAGCGTGTC 
GGAGAGTCCA GCCAGGAGCC CAAATGTCCA TCAAAGGTGG AAAAAGAAGA CAGAGGCTCG 
CTTGTGTCTC TGACTGAAGA AGAGCAGGAG TCAGATCTAG GCGACTGCAG CAGTTTGGGC 
AGTCAGAAGT CCATCCAGTC GGGTCGGAGA TGTGTCCCAC ATTCACAACC CATCCTCACC 
AAATCTGTGT CCATGGTGGC CATCAGTCAG AAGGACCTGG ACGCAATGGG ACGGCTGCGA 
CCCAAGCGAC GAATCTCCTT CTCCTTCAGC ATCTCGCCGA TGCTCCCCAA ATCTAAAACT 
CTCTTTTCTC TTGGCTCTTC ATCCAGTGAT GAGGAGGAGG CAGTCAGATT GAGTTCATTC 
ACTGGAACAT CAGGATCATT AGAGTACAGT ATATCAGAGG AGGATCCTGG GCCATTACGG 
AGTGACAGTG AAGGCAAAAT TGGAGGAACC AAAGTGAGCA GGACATTCAG TTACCTCAAG 
AGCAAGATGA GCAAGAAGAA CAAGCAGGAG AAGGAGAAAG AGAAGAATAA GGAGCGTGAG 
CGAGATGCCA AAGACAAGGA GAGAAGAGCC TCAAACGGAC ATCTCTTCAC TACGGTCATG 
ACTGTACCCA CAATCCCCTG CCACCAGTGC AACAAACTCA TCAACACTAA AGACTCTATC 
CTCTGCACCA ATTGCAATGC ACAAGTCCAC AAAGGCTGCA AAGAGAGTCT TCCTGTCTGC 
GCTAAAGTCA AGATGAAGCA GCAAAAACAG CAGCAGACTG TTACAGATTC TGCCTCGACG 
CCTAGTGTCA CCCTCAGAAG TAAAACTCTT CCAGCAAGGG ATCGTCCGTG GTCCACAATA 
TCCATTCCCG ATGACCAGCC GGCTCCCCTC GCTCCTCCTC GGAAAAGCCC CAGCAGCATC 
ATGTCTTTCA ACAGCAACCC ACTGTCCAAG AGCATGTCCA TTAATAACAT AGCAGGACAA 
ATGGACGACA CTCCTCTTAA GAAGTTTCTG TCCCAGTCAA CGGACTCTCT TCACAAGACC 
AGTAAAGTCA CGGAGTCTAC AGAATCTCTT ACTGATGAGG GTACAGAGAT GATGGACAGC 
CAGCTGATGG GAGAATTTGA AGCTGACGCC AAAGAGCTGG AGGCTGATTC TTATAGCTTC 
ACGGTAGACA AGAAATTTCT TAAGCAGCTT AAAAAGGATG TTATCAAAAG GCAAGACGTC 
ATCTATGAGC TCATCCAGAC AGAAATGCAC CATGTAAGGA CCTTAAAGAT CATGGCTGAT 
GTTTACAGCA AAGGACTACT GAGAGAAGTT CAACTAGAAG CTCAGACGGT GGAGAAGATG 
TTCCCCATGT TGGACGATGT TCTGGATTTG CACACACAGT TCTTTTCACA CCTGCTTGAA 
AGAAAGAAGG AATCAGGGAG CCAAGAAGAG GGCGGCTTTG TCATCCAAAG GATTGGCGAT 
GTGCTGGTTT CTCAGTTCTC AGGTTCCAGT GCTGAGAGAA TGAAGAAGGT CTATGGGAAG 
TTCTGCGGCA GGCACAATGA AGCTGTGAAC TTCTACAAGG AGCTTCACAC CAAAGACAAA 
CGCTTTCAAG CTTTCATCAA GAAAAAGATG AGCAGCCCTG TTGTCCGACG GCTAAGCATT 
CCTGAGTGTA TATTGTTAGT CACACAACGA ATCACAAAAT ATCCCGTGTT ACTACAGAGA 
ATACTGCAGC ACACGAAAGA AAACGAGGAG GACCATGCAG ACGTTAGTCA AGGTCTGAAG 
CTGGTGAAGG AGGTGATTGC TGCCGTGGAC AATAAAGTGA ATGAGCATGA GAAGAAGAAG 
AGGCTAAAGG AAGTGTACAG CCGGACGGAC AACAAGTCCA TCATGAGGAT GAAGAGCGGG 
CAGATGTTTG CCAGAGAGGA TCTGTTACGT GGGCGGAAGT TAATTCACGA CGGGCCGCTG 
CAGCTCAAGA ACTCAGCTGG CAGACTCAAG GATGTCCAGG CTCTGCTGCT GAGGGACGTG 
ATTGTGTTCC TGCAGGAGAA AGATCAGAAG TACATTTTCG CCTCTTTGGA CCAGCGCTCC 
ACCGTCATCT CCCTGCAGAA GCTCATTGTG AGGGAAGTAG CCAATGAGGA GAAAGCTCTG 
TTTCTGATCA CAGCAGGCAT CGAGAAGCCC GAGATGGTAG AAGTGTACAC AAGCTCCAAA 
GAGGAGAGAA ACACATGGAT GCAGCTCATT CAGGAGGCCA TGCAGTCTAT ACTGTCCATT 
TCCTCCAGGG AGAGAGATGA TGATGAAGGT GTGCCAAGTG AAAACGAAGA GGACAGGAGA 
CTTCAGGAAA TCAAGGTCAA GGAGTTCAGA GATCAGCTGC ACAAAAGAGA CGAGCAGATC 
TTGACGTTAC TGGAGGAGAA GGTGAAGTTG TTCAGGGACA TGTGTGACTG TGGTGCACCG 
GACGAGACGG GGCTTCGTAA TCGAATGCTC TTCAGGGCCA CGACGGATGA TGTCACCAGA 
GGAGAGCCAA TCATGAAGGA GGCTCTGAAG GAAGTTGAAA CGCTGCAGGT TCTGGTTAAT 
GACAGTCTTG GCGGTGCGGT TCAGGAAGGT GTGTGCGCGG CTGGACATGT GACTTTGCCA 
CGACGAGCGG AAACATTTGG AGGTTTCGAC AGTCACCAGA TGAACATCTC TAAGAGCAAA 
GATGGAGACA GAGAGGAGCT GGAGGAGTCT GCAGAGCTCC GCAGGACTGA GTCAGACAGT 
GTGTTGAAGA AGACGAGTAA GACCAACCCT TTACTGCTGG TCAGGAGAAA CAATGAGCAG 
GTTCTTCAGA GCGTTGCTCA TCTTCATGAC CTGCTGAACA CATTACAAGC TGTGGTGGTC 
CAGCAGGACA CCTTCATCGA AGACCAGCGT CACGCTCATC CCTCCTCACG CCACCCATCC 
ATCTCCTCCT CTACCTCCTC TTTATCATCC ACCTCATCCC GGCCGAGCTC TCTAATCGAG 
CAGGAGAAAC AGCGCAGCCT GGAGAAGCAG CGGCAGGAGG TGGCCAGCCT CCAAAAACAG 
CAGGCGGCCC ACGCCGAGGA GAGGCGGCGC AGGGAAAAGC AGTGGGAGAC CCGAGAGAAA 
GAGCTAACCC AGAGGGAGAT GCAGATCCAG AGCCAAGAAG AGGAGGCGCA GAGGAGAGCC 
AGACAGCTGG AGGAGGAGAA ACAGGAGCTG CAGAGTAAGA AAGAGGAGTA TCAGAGAGAC 
CTGGCACGAC TGCGGGACAG CCAGAAGAGA CTGGAGAGAG AGAGAGAGCA GGTGCAGAGA 
GAGGCAGATG AGCTTAAACT GACTGAGGAA GTGACAAAGA ACCGGTCTCC GTCAACCACG 
TCTGAAGACT CCACGAAGTT CCAGAGCACC GGTTCATTAG ACCGTGACCC ATCAGAGATG 
GAGCTCTCCT CCTCCCCGTC AGCCAGAGAC TTCCTGTCCC GCATGGACTC CAAACGCAAA 
GGGAAAAACC TCAATCCATT CTCATCCAAC TCAAGCCAGA AGAGCCAGAA CAGCGAGGCT 
CAGAGCCAGA TGCCCAGCCG TCTGCTCCAG CTGGCCAAAC CCAAGGAGAA GAAGGACAAG 
AAGAAGAAGA AGGGGAAAGG CCAGCAGAGC CAGACTGCAG ACTCTCAGAG CACTGTTGTT 
CCAGGCGCTA CCCCAGACGG AGAGATCTTT TTCTGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_021326305.1
CDS	398..8056
Translation	MKLNPQQAPLYGECLLTVQLCEDECVEDEDDVEFYLLFAGSTQRHVSSTLKISHVTLQAVCPAHDRSETVRVTLCQARPGGSVDPVSEECFQFVQDLALDMAQFLVNAAGKTEGALLLDECQIPLKECERLDDTLALALRHLPLPAGWSVLGTDLDTGTCTGSGIEPGPHDTLLHFAAKRGLRKVALFLLQQPGGREALRLPNKQGRTPAKVAQKRGHSQLHKLLTEVDNLPELETKTSKWCYPEGRVLQHHPKLNTYTLTLEDVPRSSPPSLQCDVEELQRLIQSHQKEDPPTPHKSALLILNRDSSNSTETDDCLPPNSVEPEHQEHNKQEETCKSLLNSLTFEDGLCVHQNGTGDHVQLLDGDVEPDLPAVGDTGNALDKTDCLQRRVDGDSVAFSAPSCGNQREEADIELCIISAKQGVASQPDKGKQEEQEACQKRSETEKDYNTESGNLGLQDMGHAQSQGLLPTETGQPTQREVESEGSTDVCWRGDLPEEREGDMSSNQEIDSYSSALSSHNLEGNSQHDGETEPQLPTQCSEGTDEAKLTLIQVNSEVTLSIRSEDTVISDSVDKPHGLLETETGNLSEHTKGNTESAADLVADDASVKQNSGFSVLEGDLLESLSLRPNMAPGPVHKVQQGLSPVPETSSHVGPALDPFSETTESFQQPNQSTQLLETALECEDVSLNNSTGDINAIAGVSVDISAEVSLCQNEGCPPDGSKEEVAPSERIPDLKELTEEPESVEDIIVSDVSDLDDKSQPFEESSLPIDSECFIRGDFQNDESTVQSSECTGGDINVGTLLGEEEATEKIEQPDSSLKLVELIPEEYTGDATNENKKIEPEEQSILLPTLQGESGFKDVATEEPVQEREIDSTSEETISDLPDPSEITNNDCAESNAHCQLSESSKESANTSSEPAECVNDLDLSCALPQTEIEGSRPSEGPLIDGVTICAEIPIDDKGVDMATNMSKTTISEDCLSSPMDTTSCSVLESSSMSDPLNASSSTAELTSPLDINSGLHQDLEHTSPVDIDNGLPEEPPPSDTPDCLNGLHQDSPDSLMEHSGTPQPCSEEQCVDQITDDSTSAEDAADGHQDSSVDIANSEAIHRETETFYSVDASQDSVLQEKKSSSVSSEIPSLTPSPESIRHKESPSTMRGSASDGDSLFSQDLGEDSVFNNKAEDSITITSGVSMSYSSSTDDSSSLGTTCATPQTGTEAPASDPFQEQPTSTGASGETEEEEKKDRLTEVPERSAILRTTIRSLSPFRRHSWGPGKNLAGETEMSQRSAAQGEVERRSVGHRRSMSWCPSVVLRSENDEMNERSYSLEGLAPEKEGRKSLVQQRVGESSQEPKCPSKVEKEDRGSLVSLTEEEQESDLGDCSSLGSQKSIQSGRRCVPHSQPILTKSVSMVAISQKDLDAMGRLRPKRRISFSFSISPMLPKSKTLFSLGSSSSDEEEAVRLSSFTGTSGSLEYSISEEDPGPLRSDSEGKIGGTKVSRTFSYLKSKMSKKNKQEKEKEKNKERERDAKDKERRASNGHLFTTVMTVPTIPCHQCNKLINTKDSILCTNCNAQVHKGCKESLPVCAKVKMKQQKQQQTVTDSASTPSVTLRSKTLPARDRPWSTISIPDDQPAPLAPPRKSPSSIMSFNSNPLSKSMSINNIAGQMDDTPLKKFLSQSTDSLHKTSKVTESTESLTDEGTEMMDSQLMGEFEADAKELEADSYSFTVDKKFLKQLKKDVIKRQDVIYELIQTEMHHVRTLKIMADVYSKGLLREVQLEAQTVEKMFPMLDDVLDLHTQFFSHLLERKKESGSQEEGGFVIQRIGDVLVSQFSGSSAERMKKVYGKFCGRHNEAVNFYKELHTKDKRFQAFIKKKMSSPVVRRLSIPECILLVTQRITKYPVLLQRILQHTKENEEDHADVSQGLKLVKEVIAAVDNKVNEHEKKKRLKEVYSRTDNKSIMRMKSGQMFAREDLLRGRKLIHDGPLQLKNSAGRLKDVQALLLRDVIVFLQEKDQKYIFASLDQRSTVISLQKLIVREVANEEKALFLITAGIEKPEMVEVYTSSKEERNTWMQLIQEAMQSILSISSRERDDDEGVPSENEEDRRLQEIKVKEFRDQLHKRDEQILTLLEEKVKLFRDMCDCGAPDETGLRNRMLFRATTDDVTRGEPIMKEALKEVETLQVLVNDSLGGAVQEGVCAAGHVTLPRRAETFGGFDSHQMNISKSKDGDREELEESAELRRTESDSVLKKTSKTNPLLLVRRNNEQVLQSVAHLHDLLNTLQAVVVQQDTFIEDQRHAHPSSRHPSISSSTSSLSSTSSRPSSLIEQEKQRSLEKQRQEVASLQKQQAAHAEERRRREKQWETREKELTQREMQIQSQEEEAQRRARQLEEEKQELQSKKEEYQRDLARLRDSQKRLEREREQVQREADELKLTEEVTKNRSPSTTSEDSTKFQSTGSLDRDPSEMELSSSPSARDFLSRMDSKRKGKNLNPFSSNSSQKSQNSEAQSQMPSRLLQLAKPKEKKDKKKKKGKGQQSQTADSQSTVVPGATPDGEIFFC

Target ORF information:

RefSeq Version	XM_021470630.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio wu:fi15d04 (wu:fi15d04), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_021470630.1

ORF Insert Sequence:

ATGAAACTGA ATCCACAGCA AGCCCCATTA TATGGAGAGT GTCTGCTGAC GGTCCAGCTC 
TGTGAGGATG AGTGTGTTGA GGATGAGGAT GATGTGGAGT TTTACCTGCT CTTTGCTGGA 
TCCACACAGA GACATGTGTC CAGCACACTG AAGATCAGCC ATGTCACGCT GCAGGCCGTC 
TGTCCAGCTC ATGATCGCAG TGAAACGGTA CGGGTCACAC TATGCCAGGC TCGTCCCGGC 
GGCTCAGTGG ACCCAGTGTC CGAGGAGTGT TTCCAGTTTG TCCAGGACCT GGCTCTGGAC 
ATGGCCCAGT TCCTGGTAAA TGCGGCGGGG AAAACAGAAG GAGCGCTGCT GCTGGACGAG 
TGTCAGATCC CACTGAAAGA GTGCGAGCGG CTGGACGATA CTCTTGCTCT GGCTCTCCGG 
CATCTGCCTT TGCCTGCTGG ATGGAGCGTT TTGGGCACAG ATTTGGACAC GGGCACTTGC 
ACAGGCTCGG GCATCGAGCC TGGACCACAC GATACACTTT TGCACTTCGC TGCGAAGCGC 
GGACTACGCA AGGTTGCACT CTTTCTGCTC CAGCAACCAG GGGGAAGAGA GGCTCTCCGG 
CTTCCCAACA AACAGGGTCG CACACCGGCC AAGGTCGCCC AGAAGAGGGG ACACTCTCAG 
CTCCACAAAC TGCTCACGGA GGTTGACAAT CTGCCCGAGC TGGAGACGAA AACATCTAAG 
TGGTGTTACC CAGAAGGCAG AGTCCTGCAA CACCACCCCA AGCTAAACAC TTACACCCTT 
ACCCTGGAGG ATGTGCCCAG GAGCTCCCCA CCAAGCCTTC AGTGTGATGT GGAGGAGCTC 
CAGCGTCTCA TTCAGTCACA TCAGAAGGAA GATCCTCCAA CACCGCATAA GTCAGCACTG 
CTGATTCTCA ATAGAGACTC CAGTAATAGC ACAGAGACAG ATGACTGCCT TCCGCCCAAC 
TCAGTGGAAC CTGAGCATCA GGAGCACAAC AAACAAGAAG AAACTTGCAA ATCGCTTTTA 
AACAGCCTTA CCTTTGAGGA TGGATTGTGT GTTCATCAAA ACGGCACAGG AGATCATGTC 
CAGTTGCTGG ATGGGGACGT GGAGCCTGAC CTGCCAGCTG TAGGAGATAC AGGGAATGCA 
TTAGATAAGA CTGACTGTTT GCAGCGGCGG GTAGATGGTG ATTCTGTGGC TTTCAGTGCC 
CCTTCCTGTG GAAACCAGCG GGAGGAAGCC GATATAGAGC TCTGCATAAT CAGTGCCAAG 
CAGGGGGTTG CTTCACAGCC TGATAAAGGG AAGCAAGAAG AGCAAGAGGC TTGCCAGAAG 
AGGTCAGAGA CTGAAAAGGA CTATAACACT GAATCTGGCA ACCTTGGGCT TCAAGACATG 
GGCCACGCAC AATCTCAGGG TCTCCTCCCC ACGGAAACTG GTCAGCCTAC ACAAAGGGAG 
GTGGAGAGTG AGGGGAGTAC AGATGTCTGT TGGCGTGGGG ACTTACCAGA GGAAAGGGAA 
GGGGACATGA GTAGCAACCA GGAAATAGAC TCATATTCTT CAGCACTTTC TTCCCATAAT 
CTAGAGGGAA ACAGCCAGCA CGATGGTGAG ACTGAGCCCC AGTTGCCTAC ACAATGCTCA 
GAGGGAACAG ATGAAGCTAA ATTGACTTTA ATTCAGGTAA ATTCTGAAGT TACTTTGTCT 
ATACGAAGCG AGGATACCGT TATTTCTGAC AGTGTTGACA AGCCACATGG TCTCTTAGAG 
ACGGAGACAG GAAATCTCAG CGAACACACC AAAGGCAACA CTGAGTCAGC TGCTGATTTG 
GTTGCGGATG ACGCTAGTGT AAAGCAGAAC TCTGGATTTA GCGTTTTAGA GGGGGATCTT 
TTGGAATCAT TAAGTCTACG GCCAAACATG GCACCGGGAC CAGTGCATAA GGTTCAGCAA 
GGTCTGTCAC CAGTTCCTGA GACTTCCTCT CATGTAGGTC CAGCTTTGGA CCCCTTTTCT 
GAGACTACAG AAAGTTTTCA ACAGCCGAAT CAATCCACAC AGCTTTTAGA AACTGCATTA 
GAATGTGAGG ATGTTTCATT AAATAACTCA ACCGGTGACA TTAATGCAAT AGCAGGGGTT 
TCTGTTGACA TTTCTGCAGA GGTTTCACTT TGCCAGAATG AAGGGTGTCC TCCTGATGGA 
AGCAAAGAAG AGGTAGCCCC CAGTGAGAGG ATACCAGACC TTAAAGAGCT TACGGAAGAG 
CCGGAATCAG TTGAGGACAT TATTGTCTCA GATGTCTCAG ATTTGGATGA CAAATCTCAA 
CCTTTTGAAG AGTCTTCTTT GCCTATCGAC TCTGAATGTT TTATAAGAGG TGATTTCCAA 
AACGATGAGT CTACAGTACA GTCTTCTGAA TGTACTGGAG GTGACATAAA TGTAGGAACA 
CTCTTAGGAG AAGAAGAGGC AACTGAAAAA ATAGAACAGC CAGACTCTTC ACTTAAACTG 
GTTGAGCTAA TTCCTGAGGA ATATACTGGA GATGCCACAA ACGAAAATAA AAAAATTGAG 
CCAGAGGAAC AATCTATTTT ACTGCCAACT TTGCAAGGGG AATCAGGATT TAAGGATGTT 
GCCACAGAGG AACCTGTACA GGAAAGGGAA ATTGACTCCA CTTCAGAGGA AACAATCAGC 
GATCTCCCTG ATCCATCGGA AATCACAAAC AATGATTGTG CTGAATCCAA TGCACACTGC 
CAGCTCTCAG AATCCTCCAA GGAGAGCGCA AACACATCCT CAGAACCTGC TGAATGTGTG 
AATGACCTAG ACTTGTCTTG TGCTTTACCT CAAACAGAAA TCGAAGGCAG CAGACCTTCT 
GAGGGCCCCT TAATTGATGG AGTAACAATA TGTGCTGAAA TTCCCATTGA TGACAAAGGT 
GTTGACATGG CTACCAATAT GAGTAAGACG ACCATCTCTG AGGATTGTCT GTCCTCTCCT 
ATGGACACCA CCAGCTGTTC AGTTCTGGAG AGCTCCTCCA TGTCTGACCC ACTCAATGCC 
TCATCCTCAA CAGCTGAGCT CACTTCCCCT TTGGATATTA ATAGTGGATT GCATCAGGAT 
TTGGAACACA CTTCCCCAGT GGATATAGAC AATGGTTTGC CTGAGGAACC GCCGCCGTCA 
GACACACCTG ATTGCCTGAA TGGATTACAT CAAGATTCTC CTGATAGTTT GATGGAGCAC 
AGTGGAACTC CTCAGCCATG TTCTGAGGAA CAGTGTGTGG ATCAAATCAC AGATGACAGC 
ACTTCAGCAG AGGATGCAGC GGACGGACAC CAAGACTCCT CAGTAGACAT AGCAAATTCC 
GAAGCGATCC ACAGAGAGAC CGAAACATTT TATTCAGTTG ATGCCAGTCA AGACTCTGTA 
CTCCAGGAGA AGAAATCTTC TTCAGTGAGC TCAGAAATCC CCTCTTTGAC CCCCTCACCT 
GAGAGCATAC GTCACAAAGA GTCGCCAAGC ACTATGCGAG GGTCCGCCAG CGATGGAGAC 
TCTCTGTTTA GTCAGGATCT GGGCGAGGAC AGCGTTTTTA ATAATAAGGC TGAGGACAGC 
ATTACCATCA CCAGTGGCGT GTCCATGTCC TATTCGTCCT CCACAGATGA CTCTTCCAGC 
TTGGGAACCA CTTGCGCCAC CCCTCAGACT GGCACAGAGG CTCCTGCATC AGACCCCTTT 
CAGGAACAGC CCACATCTAC TGGAGCTTCT GGGGAAACAG AGGAGGAAGA GAAAAAGGAC 
CGTCTCACTG AGGTTCCGGA GCGATCGGCC ATTTTACGGA CCACCATACG ATCACTGTCC 
CCTTTTCGAA GACACAGTTG GGGGCCGGGC AAAAATTTGG CAGGGGAGAC AGAGATGAGT 
CAGCGCAGTG CGGCACAAGG TGAAGTGGAG CGCAGATCTG TTGGTCATAG GAGAAGCATG 
AGCTGGTGTC CTTCTGTTGT CCTGCGCTCT GAAAATGATG AAATGAATGA AAGAAGTTAC 
AGTCTGGAAG GACTTGCACC AGAGAAGGAG GGAAGGAAAA GTCTGGTTCA GCAGCGTGTC 
GGAGAGTCCA GCCAGGAGCC CAAATGTCCA TCAAAGGTGG AAAAAGAAGA CAGAGGCTCG 
CTTGTGTCTC TGACTGAAGA AGAGCAGGAG TCAGATCTAG GCGACTGCAG CAGTTTGGGC 
AGTCAGAAGT CCATCCAGTC GGGTCGGAGA TGTGTCCCAC ATTCACAACC CATCCTCACC 
AAATCTGTGT CCATGGTGGC CATCAGTCAG AAGGACCTGG ACGCAATGGG ACGGCTGCGA 
CCCAAGCGAC GAATCTCCTT CTCCTTCAGC ATCTCGCCGA TGCTCCCCAA ATCTAAAACT 
CTCTTTTCTC TTGGCTCTTC ATCCAGTGAT GAGGAGGAGG CAGTCAGATT GAGTTCATTC 
ACTGGAACAT CAGGATCATT AGAGTACAGT ATATCAGAGG AGGATCCTGG GCCATTACGG 
AGTGACAGTG AAGGCAAAAT TGGAGGAACC AAAGTGAGCA GGACATTCAG TTACCTCAAG 
AGCAAGATGA GCAAGAAGAA CAAGCAGGAG AAGGAGAAAG AGAAGAATAA GGAGCGTGAG 
CGAGATGCCA AAGACAAGGA GAGAAGAGCC TCAAACGGAC ATCTCTTCAC TACGGTCATG 
ACTGTACCCA CAATCCCCTG CCACCAGTGC AACAAACTCA TCAACACTAA AGACTCTATC 
CTCTGCACCA ATTGCAATGC ACAAGTCCAC AAAGGCTGCA AAGAGAGTCT TCCTGTCTGC 
GCTAAAGTCA AGATGAAGCA GCAAAAACAG CAGCAGACTG TTACAGATTC TGCCTCGACG 
CCTAGTGTCA CCCTCAGAAG TAAAACTCTT CCAGCAAGGG ATCGTCCGTG GTCCACAATA 
TCCATTCCCG ATGACCAGCC GGCTCCCCTC GCTCCTCCTC GGAAAAGCCC CAGCAGCATC 
ATGTCTTTCA ACAGCAACCC ACTGTCCAAG AGCATGTCCA TTAATAACAT AGCAGGACAA 
ATGGACGACA CTCCTCTTAA GAAGTTTCTG TCCCAGTCAA CGGACTCTCT TCACAAGACC 
AGTAAAGTCA CGGAGTCTAC AGAATCTCTT ACTGATGAGG GTACAGAGAT GATGGACAGC 
CAGCTGATGG GAGAATTTGA AGCTGACGCC AAAGAGCTGG AGGCTGATTC TTATAGCTTC 
ACGGTAGACA AGAAATTTCT TAAGCAGCTT AAAAAGGATG TTATCAAAAG GCAAGACGTC 
ATCTATGAGC TCATCCAGAC AGAAATGCAC CATGTAAGGA CCTTAAAGAT CATGGCTGAT 
GTTTACAGCA AAGGACTACT GAGAGAAGTT CAACTAGAAG CTCAGACGGT GGAGAAGATG 
TTCCCCATGT TGGACGATGT TCTGGATTTG CACACACAGT TCTTTTCACA CCTGCTTGAA 
AGAAAGAAGG AATCAGGGAG CCAAGAAGAG GGCGGCTTTG TCATCCAAAG GATTGGCGAT 
GTGCTGGTTT CTCAGTTCTC AGGTTCCAGT GCTGAGAGAA TGAAGAAGGT CTATGGGAAG 
TTCTGCGGCA GGCACAATGA AGCTGTGAAC TTCTACAAGG AGCTTCACAC CAAAGACAAA 
CGCTTTCAAG CTTTCATCAA GAAAAAGATG AGCAGCCCTG TTGTCCGACG GCTAAGCATT 
CCTGAGTGTA TATTGTTAGT CACACAACGA ATCACAAAAT ATCCCGTGTT ACTACAGAGA 
ATACTGCAGC ACACGAAAGA AAACGAGGAG GACCATGCAG ACGTTAGTCA AGGTCTGAAG 
CTGGTGAAGG AGGTGATTGC TGCCGTGGAC AATAAAGTGA ATGAGCATGA GAAGAAGAAG 
AGGCTAAAGG AAGTGTACAG CCGGACGGAC AACAAGTCCA TCATGAGGAT GAAGAGCGGG 
CAGATGTTTG CCAGAGAGGA TCTGTTACGT GGGCGGAAGT TAATTCACGA CGGGCCGCTG 
CAGCTCAAGA ACTCAGCTGG CAGACTCAAG GATGTCCAGG CTCTGCTGCT GAGGGACGTG 
ATTGTGTTCC TGCAGGAGAA AGATCAGAAG TACATTTTCG CCTCTTTGGA CCAGCGCTCC 
ACCGTCATCT CCCTGCAGAA GCTCATTGTG AGGGAAGTAG CCAATGAGGA GAAAGCTCTG 
TTTCTGATCA CAGCAGGCAT CGAGAAGCCC GAGATGGTAG AAGTGTACAC AAGCTCCAAA 
GAGGAGAGAA ACACATGGAT GCAGCTCATT CAGGAGGCCA TGCAGTCTAT ACTGTCCATT 
TCCTCCAGGG AGAGAGATGA TGATGAAGGT GTGCCAAGTG AAAACGAAGA GGACAGGAGA 
CTTCAGGAAA TCAAGGTCAA GGAGTTCAGA GATCAGCTGC ACAAAAGAGA CGAGCAGATC 
TTGACGTTAC TGGAGGAGAA GGTGAAGTTG TTCAGGGACA TGTGTGACTG TGGTGCACCG 
GACGAGACGG GGCTTCGTAA TCGAATGCTC TTCAGGGCCA CGACGGATGA TGTCACCAGA 
GGAGAGCCAA TCATGAAGGA GGCTCTGAAG GAAGTTGAAA CGCTGCAGGT TCTGGTTAAT 
GACAGTCTTG GCGGTGCGGT TCAGGAAGGT GTGTGCGCGG CTGGACATGT GACTTTGCCA 
CGACGAGCGG AAACATTTGG AGGTTTCGAC AGTCACCAGA TGAACATCTC TAAGAGCAAA 
GATGGAGACA GAGAGGAGCT GGAGGAGTCT GCAGAGCTCC GCAGGACTGA GTCAGACAGT 
GTGTTGAAGA AGACGAGTAA GACCAACCCT TTACTGCTGG TCAGGAGAAA CAATGAGCAG 
GTTCTTCAGA GCGTTGCTCA TCTTCATGAC CTGCTGAACA CATTACAAGC TGTGGTGGTC 
CAGCAGGACA CCTTCATCGA AGACCAGCGT CACGCTCATC CCTCCTCACG CCACCCATCC 
ATCTCCTCCT CTACCTCCTC TTTATCATCC ACCTCATCCC GGCCGAGCTC TCTAATCGAG 
CAGGAGAAAC AGCGCAGCCT GGAGAAGCAG CGGCAGGAGG TGGCCAGCCT CCAAAAACAG 
CAGGCGGCCC ACGCCGAGGA GAGGCGGCGC AGGGAAAAGC AGTGGGAGAC CCGAGAGAAA 
GAGCTAACCC AGAGGGAGAT GCAGATCCAG AGCCAAGAAG AGGAGGCGCA GAGGAGAGCC 
AGACAGCTGG AGGAGGAGAA ACAGGAGCTG CAGAGTAAGA AAGAGGAGTA TCAGAGAGAC 
CTGGCACGAC TGCGGGACAG CCAGAAGAGA CTGGAGAGAG AGAGAGAGCA GGTGCAGAGA 
GAGGCAGATG AGCTTAAACT GACTGAGGAA GTGACAAAGA ACCGGTCTCC GTCAACCACG 
TCTGAAGACT CCACGAAGTT CCAGAGCACC GGTTCATTAG ACCGTGACCC ATCAGAGATG 
GAGCTCTCCT CCTCCCCGTC AGCCAGAGAC TTCCTGTCCC GCATGGACTC CAAACGCAAA 
GGGAAAAACC TCAATCCATT CTCATCCAAC TCAAGCCAGA AGAGCCAGAA CAGCGAGGCT 
CAGAGCCAGA TGCCCAGCCG TCTGCTCCAG CTGGCCAAAC CCAAGGAGAA GAAGGACAAG 
AAGAAGAAGA AGGGGAAAGG CCAGCAGAGC CAGACTGCAG ACTCTCAGAG CACTGTTGTT 
CCAGGCGCTA CCCCAGACGG AGAGATCTTT TTCTGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

wu:fi15d04 ( XM_021470631.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_021326306.1 Danio rerio wu:fi15d04 (wu:fi15d04), transcript variant X3, mRNA.

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CloneID	ODa141426
Clone ID Related Accession (Same CDS sequence)	XM_021470631.1
Accession Version	XM_021470631.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7659bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	A-kinase anchor protein 13 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007136.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAACTGA ATCCACAGCA AGCCCCATTA TATGGAGAGT GTCTGCTGAC GGTCCAGCTC 
TGTGAGGATG AGTGTGTTGA GGATGAGGAT GATGTGGAGT TTTACCTGCT CTTTGCTGGA 
TCCACACAGA GACATGTGTC CAGCACACTG AAGATCAGCC ATGTCACGCT GCAGGCCGTC 
TGTCCAGCTC ATGATCGCAG TGAAACGGTA CGGGTCACAC TATGCCAGGC TCGTCCCGGC 
GGCTCAGTGG ACCCAGTGTC CGAGGAGTGT TTCCAGTTTG TCCAGGACCT GGCTCTGGAC 
ATGGCCCAGT TCCTGGTAAA TGCGGCGGGG AAAACAGAAG GAGCGCTGCT GCTGGACGAG 
TGTCAGATCC CACTGAAAGA GTGCGAGCGG CTGGACGATA CTCTTGCTCT GGCTCTCCGG 
CATCTGCCTT TGCCTGCTGG ATGGAGCGTT TTGGGCACAG ATTTGGACAC GGGCACTTGC 
ACAGGCTCGG GCATCGCAGA GCCTGGACCA CACGATACAC TTTTGCACTT CGCTGCGAAG 
CGCGGACTAC GCAAGGTTGC ACTCTTTCTG CTCCAGCAAC CAGGGGGAAG AGAGGCTCTC 
CGGCTTCCCA ACAAACAGGG TCGCACACCG GCCAAGGTCG CCCAGAAGAG GGGACACTCT 
CAGCTCCACA AACTGCTCAC GGAGGTTGAC AATCTGCCCG AGCTGGAGAC GAAAACATCT 
AAGTGGTGTT ACCCAGAAGG CAGAGTCCTG CAACACCACC CCAAGCTAAA CACTTACACC 
CTTACCCTGG AGGATGTGCC CAGGAGCTCC CCACCAAGCC TTCAGTGTGA TGTGGAGGAG 
CTCCAGCGTC TCATTCAGTC ACATCAGAAG GAAGATCCTC CAACACCGCA TAAGTCAGCA 
CTGCTGATTC TCAATAGAGA CTCCAGTAAT AGCACAGAGA CAGATGACTG CCTTCCGCCC 
AACTCAGTGG AACCTGAGCA TCAGGAGCAC AACAAACAAG AAGAAACTTG CAAATCGCTT 
TTAAACAGCC TTACCTTTGA GGATGGATTG TGTGTTCATC AAAACGGCAC AGGAGATCAT 
GTCCAGTTGC TGGATGGGGA CGTGGAGCCT GACCTGCCAG CTGTAGGAGA TACAGGGAAT 
GCATTAGATA AGACTGACTG TTTGCAGCGG CGGGTAGATG GTGATTCTGT GGCTTTCAGT 
GCCCCTTCCT GTGGAAACCA GCGGGAGGAA GCCGATATAG AGCTCTGCAT AATCAGTGCC 
AAGCAGGGGG TTGCTTCACA GCCTGATAAA GGGAAGCAAG AAGAGCAAGA GGCTTGCCAG 
AAGAGGTCAG AGACTGAAAA GGACTATAAC ACTGAATCTG GCAACCTTGG GCTTCAAGAC 
ATGGGCCACG CACAATCTCA GGGTCTCCTC CCCACGGAAA CTGGTCAGCC TACACAAAGG 
GAGGTGGAGA GTGAGGGGAG TACAGATGTC TGTTGGCGTG GGGACTTACC AGAGGAAAGG 
GAAGGGGACA TGAGTAGCAA CCAGGAAATA GACTCATATT CTTCAGCACT TTCTTCCCAT 
AATCTAGAGG GAAACAGCCA GCACGATGGT GAGACTGAGC CCCAGTTGCC TACACAATGC 
TCAGAGGGAA CAGATGAAGC TAAATTGACT TTAATTCAGG TAAATTCTGA AGTTACTTTG 
TCTATACGAA GCGAGGATAC CGTTATTTCT GACAGTGTTG ACAAGCCACA TGGTCTCTTA 
GAGACGGAGA CAGGAAATCT CAGCGAACAC ACCAAAGGCA ACACTGAGTC AGCTGCTGAT 
TTGGTTGCGG ATGACGCTAG TGTAAAGCAG AACTCTGGAT TTAGCGTTTT AGAGGGGGAT 
CTTTTGGAAT CATTAAGTCT ACGGCCAAAC ATGGCACCGG GACCAGTGCA TAAGGTTCAG 
CAAGGTCTGT CACCAGTTCC TGAGACTTCC TCTCATGTAG GTCCAGCTTT GGACCCCTTT 
TCTGAGACTA CAGAAAGTTT TCAACAGCCG AATCAATCCA CACAGCTTTT AGAAACTGCA 
TTAGAATGTG AGGATGTTTC ATTAAATAAC TCAACCGGTG ACATTAATGC AATAGCAGGG 
GTTTCTGTTG ACATTTCTGC AGAGGTTTCA CTTTGCCAGA ATGAAGGGTG TCCTCCTGAT 
GGAAGCAAAG AAGAGGTAGC CCCCAGTGAG AGGATACCAG ACCTTAAAGA GCTTACGGAA 
GAGCCGGAAT CAGTTGAGGA CATTATTGTC TCAGATGTCT CAGATTTGGA TGACAAATCT 
CAACCTTTTG AAGAGTCTTC TTTGCCTATC GACTCTGAAT GTTTTATAAG AGGTGATTTC 
CAAAACGATG AGTCTACAGT ACAGTCTTCT GAATGTACTG GAGGTGACAT AAATGTAGGA 
ACACTCTTAG GAGAAGAAGA GGCAACTGAA AAAATAGAAC AGCCAGACTC TTCACTTAAA 
CTGGTTGAGC TAATTCCTGA GGAATATACT GGAGATGCCA CAAACGAAAA TAAAAAAATT 
GAGCCAGAGG AACAATCTAT TTTACTGCCA ACTTTGCAAG GGGAATCAGG ATTTAAGGAT 
GTTGCCACAG AGGAACCTGT ACAGGAAAGG GAAATTGACT CCACTTCAGA GGAAACAATC 
AGCGATCTCC CTGATCCATC GGAAATCACA AACAATGATT GTGCTGAATC CAATGCACAC 
TGCCAGCTCT CAGAATCCTC CAAGGAGAGC GCAAACACAT CCTCAGAACC TGCTGAATGT 
GTGAATGACC TAGACTTGTC TTGTGCTTTA CCTCAAACAG AAATCGAAGG CAGCAGACCT 
TCTGAGGGCC CCTTAATTGA TGGAGTAACA ATATGTGCTG AAATTCCCAT TGATGACAAA 
GGTGTTGACA TGGCTACCAA TATGAGTAAG ACGACCATCT CTGAGGATTG TCTGTCCTCT 
CCTATGGACA CCACCAGCTG TTCAGTTCTG GAGAGCTCCT CCATGTCTGA CCCACTCAAT 
GCCTCATCCT CAACAGCTGA GCTCACTTCC CCTTTGGATA TTAATAGTGG ATTGCATCAG 
GATTTGGAAC ACACTTCCCC AGTGGATATA GACAATGGTT TGCCTGAGGA ACCGCCGCCG 
TCAGACACAC CTGATTGCCT GAATGGATTA CATCAAGATT CTCCTGATAG TTTGATGGAG 
CACAGTGGAA CTCCTCAGCC ATGTTCTGAG GAACAGTGTG TGGATCAAAT CACAGATGAC 
AGCACTTCAG CAGAGGATGC AGCGGACGGA CACCAAGACT CCTCAGTAGA CATAGCAAAT 
TCCGAAGCGA TCCACAGAGA GACCGAAACA TTTTATTCAG TTGATGCCAG TCAAGACTCT 
GTACTCCAGG AGAAGAAATC TTCTTCAGTG AGCTCAGAAA TCCCCTCTTT GACCCCCTCA 
CCTGAGAGCA TACGTCACAA AGAGTCGCCA AGCACTATGC GAGGGTCCGC CAGCGATGGA 
GACTCTCTGT TTAGTCAGGA TCTGGGCGAG GACAGCGTTT TTAATAATAA GGCTGAGGAC 
AGCATTACCA TCACCAGTGG CGTGTCCATG TCCTATTCGT CCTCCACAGA TGACTCTTCC 
AGCTTGGGAA CCACTTGCGC CACCCCTCAG ACTGGCACAG AGGCTCCTGC ATCAGACCCC 
TTTCAGGAAC AGCCCACATC TACTGGAGCT TCTGGGGAAA CAGAGGAGGA AGAGAAAAAG 
GACCGTCTCA CTGAGGTTCC GGAGCGATCG GCCATTTTAC GGACCACCAT ACGATCACTG 
TCCCCTTTTC GAAGACACAG TTGGGGGCCG GGCAAAAATT TGGCAGGGGA GACAGAGATG 
AGTCAGCGCA GTGCGGCACA AGGTGAAGTG GAGCGCAGAT CTGTTGGTCA TAGGAGAAGC 
ATGAGCTGGT GTCCTTCTGT TGTCCTGCGC TCTGAAAATG ATGAAATGAA TGAAAGAAGT 
TACAGTCTGG AAGGACTTGC ACCAGAGAAG GAGGGAAGGA AAAGTCTGGT TCAGCAGCGT 
GTCGGAGAGT CCAGCCAGGA GCCCAAATGT CCATCAAAGG TGGAAAAAGA AGACAGAGGC 
TCGCTTGTGT CTCTGACTGA AGAAGAGCAG GAGTCAGATC TAGGCGACTG CAGCAGTTTG 
GGCAGTCAGA AGTCCATCCA GTCGGGTCGG AGATGTGTCC CACATTCACA ACCCATCCTC 
ACCAAATCTG TGTCCATGGT GGCCATCAGT CAGAAGGACC TGGACGCAAT GGGACGGCTG 
CGACCCAAGC GACGAATCTC CTTCTCCTTC AGCATCTCGC CGATGCTCCC CAAATCTAAA 
ACTCTCTTTT CTCTTGGCTC TTCATCCAGT GATGAGGAGG AGGCAGTCAG ATTGAGTTCA 
TTCACTGGAA CATCAGGATC ATTAGAGTAC AGTATATCAG AGGAGGATCC TGGGCCATTA 
CGGAGTGACA GTGAAGGCAA AATTGGAGGA ACCAAAGTGA GCAGGACATT CAGTTACCTC 
AAGAGCAAGA TGAGCAAGAA GAACAAGGAG AAGGAGAAAG AGAAGAATAA GGAGCGTGAG 
CGAGATGCCA AAGACAAGGA GAGAAGAGCC TCAAACGGAC ATCTCTTCAC TACGGTCATG 
ACTGTACCCA CAATCCCCTG CCACCAGTGC AACAAACTCA TCAACACTAA AGACTCTATC 
CTCTGCACCA ATTGCAATGC ACAAGTCCAC AAAGGCTGCA AAGAGAGTCT TCCTGTCTGC 
GCTAAAGTCA AGATGAAGCA GCAAAAACAG CAGCAGACTG TTACAGATTC TGCCTCGACG 
CCTAGTGTCA CCCTCAGAAG TAAAACTCTT CCAGCAAGGG ATCGTCCGTG GTCCACAATA 
TCCATTCCCG ATGACCAGCC GGCTCCCCTC GCTCCTCCTC GGAAAAGCCC CAGCAGCATC 
ATGTCTTTCA ACAGCAACCC ACTGTCCAAG AGCATGTCCA TTAATAACAT AGCAGGACAA 
ATGGACGACA CTCCTCTTAA GAAGTTTCTG TCCCAGTCAA CGGACTCTCT TCACAAGACC 
AGTAAAGTCA CGGAGTCTAC AGAATCTCTT ACTGATGAGG GTACAGAGAT GATGGACAGC 
CAGCTGATGG GAGAATTTGA AGCTGACGCC AAAGAGCTGG AGGCTGATTC TTATAGCTTC 
ACGGTAGACA AGAAATTTCT TAAGCAGCTT AAAAAGGATG TTATCAAAAG GCAAGACGTC 
ATCTATGAGC TCATCCAGAC AGAAATGCAC CATGTAAGGA CCTTAAAGAT CATGGCTGAT 
GTTTACAGCA AAGGACTACT GAGAGAAGTT CAACTAGAAG CTCAGACGGT GGAGAAGATG 
TTCCCCATGT TGGACGATGT TCTGGATTTG CACACACAGT TCTTTTCACA CCTGCTTGAA 
AGAAAGAAGG AATCAGGGAG CCAAGAAGAG GGCGGCTTTG TCATCCAAAG GATTGGCGAT 
GTGCTGGTTT CTCAGTTCTC AGGTTCCAGT GCTGAGAGAA TGAAGAAGGT CTATGGGAAG 
TTCTGCGGCA GGCACAATGA AGCTGTGAAC TTCTACAAGG AGCTTCACAC CAAAGACAAA 
CGCTTTCAAG CTTTCATCAA GAAAAAGATG AGCAGCCCTG TTGTCCGACG GCTAAGCATT 
CCTGAGTGTA TATTGTTAGT CACACAACGA ATCACAAAAT ATCCCGTGTT ACTACAGAGA 
ATACTGCAGC ACACGAAAGA AAACGAGGAG GACCATGCAG ACGTTAGTCA AGGTCTGAAG 
CTGGTGAAGG AGGTGATTGC TGCCGTGGAC AATAAAGTGA ATGAGCATGA GAAGAAGAAG 
AGGCTAAAGG AAGTGTACAG CCGGACGGAC AACAAGTCCA TCATGAGGAT GAAGAGCGGG 
CAGATGTTTG CCAGAGAGGA TCTGTTACGT GGGCGGAAGT TAATTCACGA CGGGCCGCTG 
CAGCTCAAGA ACTCAGCTGG CAGACTCAAG GATGTCCAGG CTCTGCTGCT GAGGGACGTG 
ATTGTGTTCC TGCAGGAGAA AGATCAGAAG TACATTTTCG CCTCTTTGGA CCAGCGCTCC 
ACCGTCATCT CCCTGCAGAA GCTCATTGTG AGGGAAGTAG CCAATGAGGA GAAAGCTCTG 
TTTCTGATCA CAGCAGGCAT CGAGAAGCCC GAGATGGTAG AAGTGTACAC AAGCTCCAAA 
GAGGAGAGAA ACACATGGAT GCAGCTCATT CAGGAGGCCA TGCAGTCTAT ACTGTCCATT 
TCCTCCAGGG AGAGAGATGA TGATGAAGGT GTGCCAAGTG AAAACGAAGA GGACAGGAGA 
CTTCAGGAAA TCAAGGTCAA GGAGTTCAGA GATCAGCTGC ACAAAAGAGA CGAGCAGATC 
TTGACGTTAC TGGAGGAGAA GGTGAAGTTG TTCAGGGACA TGTGTGACTG TGGTGCACCG 
GACGAGACGG GGCTTCGTAA TCGAATGCTC TTCAGGGCCA CGACGGATGA TGTCACCAGA 
GGAGAGCCAA TCATGAAGGA GGCTCTGAAG GAAGTTGAAA CGCTGCAGGT TCTGGTTAAT 
GACAGTCTTG GCGGTGCGGT TCAGGAAGGT GTGTGCGCGG CTGGACATGT GACTTTGCCA 
CGACGAGCGG AAACATTTGG AGGTTTCGAC AGTCACCAGA TGAACATCTC TAAGAGCAAA 
GATGGAGACA GAGAGGAGCT GGAGGAGTCT GCAGAGCTCC GCAGGACTGA GTCAGACAGT 
GTGTTGAAGA AGACGAGTAA GACCAACCCT TTACTGCTGG TCAGGAGAAA CAATGAGCAG 
GTTCTTCAGA GCGTTGCTCA TCTTCATGAC CTGCTGAACA CATTACAAGC TGTGGTGGTC 
CAGCAGGACA CCTTCATCGA AGACCAGCGT CACGCTCATC CCTCCTCACG CCACCCATCC 
ATCTCCTCCT CTACCTCCTC TTTATCATCC ACCTCATCCC GGCCGAGCTC TCTAATCGAG 
CAGGAGAAAC AGCGCAGCCT GGAGAAGCAG CGGCAGGAGG TGGCCAGCCT CCAAAAACAG 
CAGGCGGCCC ACGCCGAGGA GAGGCGGCGC AGGGAAAAGC AGTGGGAGAC CCGAGAGAAA 
GAGCTAACCC AGAGGGAGAT GCAGATCCAG AGCCAAGAAG AGGAGGCGCA GAGGAGAGCC 
AGACAGCTGG AGGAGGAGAA ACAGGAGCTG CAGAGTAAGA AAGAGGAGTA TCAGAGAGAC 
CTGGCACGAC TGCGGGACAG CCAGAAGAGA CTGGAGAGAG AGAGAGAGCA GGTGCAGAGA 
GAGGCAGATG AGCTTAAACT GACTGAGGAA GTGACAAAGA ACCGGTCTCC GTCAACCACG 
TCTGAAGACT CCACGAAGTT CCAGAGCACC GGTTCATTAG ACCGTGACCC ATCAGAGATG 
GAGCTCTCCT CCTCCCCGTC AGCCAGAGAC TTCCTGTCCC GCATGGACTC CAAACGCAAA 
GGGAAAAACC TCAATCCATT CTCATCCAAC TCAAGCCAGA AGAGCCAGAA CAGCGAGGCT 
CAGAGCCAGA TGCCCAGCCG TCTGCTCCAG CTGGCCAAAC CCAAGGAGAA GAAGGACAAG 
AAGAAGAAGA AGGGGAAAGG CCAGCAGAGC CAGACTGCAG ACTCTCAGAG CACTGTTGTT 
CCAGGCGCTA CCCCAGACGG AGAGATCTTT TTCTGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_021326306.1
CDS	398..8056
Translation	MKLNPQQAPLYGECLLTVQLCEDECVEDEDDVEFYLLFAGSTQRHVSSTLKISHVTLQAVCPAHDRSETVRVTLCQARPGGSVDPVSEECFQFVQDLALDMAQFLVNAAGKTEGALLLDECQIPLKECERLDDTLALALRHLPLPAGWSVLGTDLDTGTCTGSGIAEPGPHDTLLHFAAKRGLRKVALFLLQQPGGREALRLPNKQGRTPAKVAQKRGHSQLHKLLTEVDNLPELETKTSKWCYPEGRVLQHHPKLNTYTLTLEDVPRSSPPSLQCDVEELQRLIQSHQKEDPPTPHKSALLILNRDSSNSTETDDCLPPNSVEPEHQEHNKQEETCKSLLNSLTFEDGLCVHQNGTGDHVQLLDGDVEPDLPAVGDTGNALDKTDCLQRRVDGDSVAFSAPSCGNQREEADIELCIISAKQGVASQPDKGKQEEQEACQKRSETEKDYNTESGNLGLQDMGHAQSQGLLPTETGQPTQREVESEGSTDVCWRGDLPEEREGDMSSNQEIDSYSSALSSHNLEGNSQHDGETEPQLPTQCSEGTDEAKLTLIQVNSEVTLSIRSEDTVISDSVDKPHGLLETETGNLSEHTKGNTESAADLVADDASVKQNSGFSVLEGDLLESLSLRPNMAPGPVHKVQQGLSPVPETSSHVGPALDPFSETTESFQQPNQSTQLLETALECEDVSLNNSTGDINAIAGVSVDISAEVSLCQNEGCPPDGSKEEVAPSERIPDLKELTEEPESVEDIIVSDVSDLDDKSQPFEESSLPIDSECFIRGDFQNDESTVQSSECTGGDINVGTLLGEEEATEKIEQPDSSLKLVELIPEEYTGDATNENKKIEPEEQSILLPTLQGESGFKDVATEEPVQEREIDSTSEETISDLPDPSEITNNDCAESNAHCQLSESSKESANTSSEPAECVNDLDLSCALPQTEIEGSRPSEGPLIDGVTICAEIPIDDKGVDMATNMSKTTISEDCLSSPMDTTSCSVLESSSMSDPLNASSSTAELTSPLDINSGLHQDLEHTSPVDIDNGLPEEPPPSDTPDCLNGLHQDSPDSLMEHSGTPQPCSEEQCVDQITDDSTSAEDAADGHQDSSVDIANSEAIHRETETFYSVDASQDSVLQEKKSSSVSSEIPSLTPSPESIRHKESPSTMRGSASDGDSLFSQDLGEDSVFNNKAEDSITITSGVSMSYSSSTDDSSSLGTTCATPQTGTEAPASDPFQEQPTSTGASGETEEEEKKDRLTEVPERSAILRTTIRSLSPFRRHSWGPGKNLAGETEMSQRSAAQGEVERRSVGHRRSMSWCPSVVLRSENDEMNERSYSLEGLAPEKEGRKSLVQQRVGESSQEPKCPSKVEKEDRGSLVSLTEEEQESDLGDCSSLGSQKSIQSGRRCVPHSQPILTKSVSMVAISQKDLDAMGRLRPKRRISFSFSISPMLPKSKTLFSLGSSSSDEEEAVRLSSFTGTSGSLEYSISEEDPGPLRSDSEGKIGGTKVSRTFSYLKSKMSKKNKEKEKEKNKERERDAKDKERRASNGHLFTTVMTVPTIPCHQCNKLINTKDSILCTNCNAQVHKGCKESLPVCAKVKMKQQKQQQTVTDSASTPSVTLRSKTLPARDRPWSTISIPDDQPAPLAPPRKSPSSIMSFNSNPLSKSMSINNIAGQMDDTPLKKFLSQSTDSLHKTSKVTESTESLTDEGTEMMDSQLMGEFEADAKELEADSYSFTVDKKFLKQLKKDVIKRQDVIYELIQTEMHHVRTLKIMADVYSKGLLREVQLEAQTVEKMFPMLDDVLDLHTQFFSHLLERKKESGSQEEGGFVIQRIGDVLVSQFSGSSAERMKKVYGKFCGRHNEAVNFYKELHTKDKRFQAFIKKKMSSPVVRRLSIPECILLVTQRITKYPVLLQRILQHTKENEEDHADVSQGLKLVKEVIAAVDNKVNEHEKKKRLKEVYSRTDNKSIMRMKSGQMFAREDLLRGRKLIHDGPLQLKNSAGRLKDVQALLLRDVIVFLQEKDQKYIFASLDQRSTVISLQKLIVREVANEEKALFLITAGIEKPEMVEVYTSSKEERNTWMQLIQEAMQSILSISSRERDDDEGVPSENEEDRRLQEIKVKEFRDQLHKRDEQILTLLEEKVKLFRDMCDCGAPDETGLRNRMLFRATTDDVTRGEPIMKEALKEVETLQVLVNDSLGGAVQEGVCAAGHVTLPRRAETFGGFDSHQMNISKSKDGDREELEESAELRRTESDSVLKKTSKTNPLLLVRRNNEQVLQSVAHLHDLLNTLQAVVVQQDTFIEDQRHAHPSSRHPSISSSTSSLSSTSSRPSSLIEQEKQRSLEKQRQEVASLQKQQAAHAEERRRREKQWETREKELTQREMQIQSQEEEAQRRARQLEEEKQELQSKKEEYQRDLARLRDSQKRLEREREQVQREADELKLTEEVTKNRSPSTTSEDSTKFQSTGSLDRDPSEMELSSSPSARDFLSRMDSKRKGKNLNPFSSNSSQKSQNSEAQSQMPSRLLQLAKPKEKKDKKKKKGKGQQSQTADSQSTVVPGATPDGEIFFC

Target ORF information:

RefSeq Version	XM_021470631.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio wu:fi15d04 (wu:fi15d04), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_021470631.1

ORF Insert Sequence:

ATGAAACTGA ATCCACAGCA AGCCCCATTA TATGGAGAGT GTCTGCTGAC GGTCCAGCTC 
TGTGAGGATG AGTGTGTTGA GGATGAGGAT GATGTGGAGT TTTACCTGCT CTTTGCTGGA 
TCCACACAGA GACATGTGTC CAGCACACTG AAGATCAGCC ATGTCACGCT GCAGGCCGTC 
TGTCCAGCTC ATGATCGCAG TGAAACGGTA CGGGTCACAC TATGCCAGGC TCGTCCCGGC 
GGCTCAGTGG ACCCAGTGTC CGAGGAGTGT TTCCAGTTTG TCCAGGACCT GGCTCTGGAC 
ATGGCCCAGT TCCTGGTAAA TGCGGCGGGG AAAACAGAAG GAGCGCTGCT GCTGGACGAG 
TGTCAGATCC CACTGAAAGA GTGCGAGCGG CTGGACGATA CTCTTGCTCT GGCTCTCCGG 
CATCTGCCTT TGCCTGCTGG ATGGAGCGTT TTGGGCACAG ATTTGGACAC GGGCACTTGC 
ACAGGCTCGG GCATCGCAGA GCCTGGACCA CACGATACAC TTTTGCACTT CGCTGCGAAG 
CGCGGACTAC GCAAGGTTGC ACTCTTTCTG CTCCAGCAAC CAGGGGGAAG AGAGGCTCTC 
CGGCTTCCCA ACAAACAGGG TCGCACACCG GCCAAGGTCG CCCAGAAGAG GGGACACTCT 
CAGCTCCACA AACTGCTCAC GGAGGTTGAC AATCTGCCCG AGCTGGAGAC GAAAACATCT 
AAGTGGTGTT ACCCAGAAGG CAGAGTCCTG CAACACCACC CCAAGCTAAA CACTTACACC 
CTTACCCTGG AGGATGTGCC CAGGAGCTCC CCACCAAGCC TTCAGTGTGA TGTGGAGGAG 
CTCCAGCGTC TCATTCAGTC ACATCAGAAG GAAGATCCTC CAACACCGCA TAAGTCAGCA 
CTGCTGATTC TCAATAGAGA CTCCAGTAAT AGCACAGAGA CAGATGACTG CCTTCCGCCC 
AACTCAGTGG AACCTGAGCA TCAGGAGCAC AACAAACAAG AAGAAACTTG CAAATCGCTT 
TTAAACAGCC TTACCTTTGA GGATGGATTG TGTGTTCATC AAAACGGCAC AGGAGATCAT 
GTCCAGTTGC TGGATGGGGA CGTGGAGCCT GACCTGCCAG CTGTAGGAGA TACAGGGAAT 
GCATTAGATA AGACTGACTG TTTGCAGCGG CGGGTAGATG GTGATTCTGT GGCTTTCAGT 
GCCCCTTCCT GTGGAAACCA GCGGGAGGAA GCCGATATAG AGCTCTGCAT AATCAGTGCC 
AAGCAGGGGG TTGCTTCACA GCCTGATAAA GGGAAGCAAG AAGAGCAAGA GGCTTGCCAG 
AAGAGGTCAG AGACTGAAAA GGACTATAAC ACTGAATCTG GCAACCTTGG GCTTCAAGAC 
ATGGGCCACG CACAATCTCA GGGTCTCCTC CCCACGGAAA CTGGTCAGCC TACACAAAGG 
GAGGTGGAGA GTGAGGGGAG TACAGATGTC TGTTGGCGTG GGGACTTACC AGAGGAAAGG 
GAAGGGGACA TGAGTAGCAA CCAGGAAATA GACTCATATT CTTCAGCACT TTCTTCCCAT 
AATCTAGAGG GAAACAGCCA GCACGATGGT GAGACTGAGC CCCAGTTGCC TACACAATGC 
TCAGAGGGAA CAGATGAAGC TAAATTGACT TTAATTCAGG TAAATTCTGA AGTTACTTTG 
TCTATACGAA GCGAGGATAC CGTTATTTCT GACAGTGTTG ACAAGCCACA TGGTCTCTTA 
GAGACGGAGA CAGGAAATCT CAGCGAACAC ACCAAAGGCA ACACTGAGTC AGCTGCTGAT 
TTGGTTGCGG ATGACGCTAG TGTAAAGCAG AACTCTGGAT TTAGCGTTTT AGAGGGGGAT 
CTTTTGGAAT CATTAAGTCT ACGGCCAAAC ATGGCACCGG GACCAGTGCA TAAGGTTCAG 
CAAGGTCTGT CACCAGTTCC TGAGACTTCC TCTCATGTAG GTCCAGCTTT GGACCCCTTT 
TCTGAGACTA CAGAAAGTTT TCAACAGCCG AATCAATCCA CACAGCTTTT AGAAACTGCA 
TTAGAATGTG AGGATGTTTC ATTAAATAAC TCAACCGGTG ACATTAATGC AATAGCAGGG 
GTTTCTGTTG ACATTTCTGC AGAGGTTTCA CTTTGCCAGA ATGAAGGGTG TCCTCCTGAT 
GGAAGCAAAG AAGAGGTAGC CCCCAGTGAG AGGATACCAG ACCTTAAAGA GCTTACGGAA 
GAGCCGGAAT CAGTTGAGGA CATTATTGTC TCAGATGTCT CAGATTTGGA TGACAAATCT 
CAACCTTTTG AAGAGTCTTC TTTGCCTATC GACTCTGAAT GTTTTATAAG AGGTGATTTC 
CAAAACGATG AGTCTACAGT ACAGTCTTCT GAATGTACTG GAGGTGACAT AAATGTAGGA 
ACACTCTTAG GAGAAGAAGA GGCAACTGAA AAAATAGAAC AGCCAGACTC TTCACTTAAA 
CTGGTTGAGC TAATTCCTGA GGAATATACT GGAGATGCCA CAAACGAAAA TAAAAAAATT 
GAGCCAGAGG AACAATCTAT TTTACTGCCA ACTTTGCAAG GGGAATCAGG ATTTAAGGAT 
GTTGCCACAG AGGAACCTGT ACAGGAAAGG GAAATTGACT CCACTTCAGA GGAAACAATC 
AGCGATCTCC CTGATCCATC GGAAATCACA AACAATGATT GTGCTGAATC CAATGCACAC 
TGCCAGCTCT CAGAATCCTC CAAGGAGAGC GCAAACACAT CCTCAGAACC TGCTGAATGT 
GTGAATGACC TAGACTTGTC TTGTGCTTTA CCTCAAACAG AAATCGAAGG CAGCAGACCT 
TCTGAGGGCC CCTTAATTGA TGGAGTAACA ATATGTGCTG AAATTCCCAT TGATGACAAA 
GGTGTTGACA TGGCTACCAA TATGAGTAAG ACGACCATCT CTGAGGATTG TCTGTCCTCT 
CCTATGGACA CCACCAGCTG TTCAGTTCTG GAGAGCTCCT CCATGTCTGA CCCACTCAAT 
GCCTCATCCT CAACAGCTGA GCTCACTTCC CCTTTGGATA TTAATAGTGG ATTGCATCAG 
GATTTGGAAC ACACTTCCCC AGTGGATATA GACAATGGTT TGCCTGAGGA ACCGCCGCCG 
TCAGACACAC CTGATTGCCT GAATGGATTA CATCAAGATT CTCCTGATAG TTTGATGGAG 
CACAGTGGAA CTCCTCAGCC ATGTTCTGAG GAACAGTGTG TGGATCAAAT CACAGATGAC 
AGCACTTCAG CAGAGGATGC AGCGGACGGA CACCAAGACT CCTCAGTAGA CATAGCAAAT 
TCCGAAGCGA TCCACAGAGA GACCGAAACA TTTTATTCAG TTGATGCCAG TCAAGACTCT 
GTACTCCAGG AGAAGAAATC TTCTTCAGTG AGCTCAGAAA TCCCCTCTTT GACCCCCTCA 
CCTGAGAGCA TACGTCACAA AGAGTCGCCA AGCACTATGC GAGGGTCCGC CAGCGATGGA 
GACTCTCTGT TTAGTCAGGA TCTGGGCGAG GACAGCGTTT TTAATAATAA GGCTGAGGAC 
AGCATTACCA TCACCAGTGG CGTGTCCATG TCCTATTCGT CCTCCACAGA TGACTCTTCC 
AGCTTGGGAA CCACTTGCGC CACCCCTCAG ACTGGCACAG AGGCTCCTGC ATCAGACCCC 
TTTCAGGAAC AGCCCACATC TACTGGAGCT TCTGGGGAAA CAGAGGAGGA AGAGAAAAAG 
GACCGTCTCA CTGAGGTTCC GGAGCGATCG GCCATTTTAC GGACCACCAT ACGATCACTG 
TCCCCTTTTC GAAGACACAG TTGGGGGCCG GGCAAAAATT TGGCAGGGGA GACAGAGATG 
AGTCAGCGCA GTGCGGCACA AGGTGAAGTG GAGCGCAGAT CTGTTGGTCA TAGGAGAAGC 
ATGAGCTGGT GTCCTTCTGT TGTCCTGCGC TCTGAAAATG ATGAAATGAA TGAAAGAAGT 
TACAGTCTGG AAGGACTTGC ACCAGAGAAG GAGGGAAGGA AAAGTCTGGT TCAGCAGCGT 
GTCGGAGAGT CCAGCCAGGA GCCCAAATGT CCATCAAAGG TGGAAAAAGA AGACAGAGGC 
TCGCTTGTGT CTCTGACTGA AGAAGAGCAG GAGTCAGATC TAGGCGACTG CAGCAGTTTG 
GGCAGTCAGA AGTCCATCCA GTCGGGTCGG AGATGTGTCC CACATTCACA ACCCATCCTC 
ACCAAATCTG TGTCCATGGT GGCCATCAGT CAGAAGGACC TGGACGCAAT GGGACGGCTG 
CGACCCAAGC GACGAATCTC CTTCTCCTTC AGCATCTCGC CGATGCTCCC CAAATCTAAA 
ACTCTCTTTT CTCTTGGCTC TTCATCCAGT GATGAGGAGG AGGCAGTCAG ATTGAGTTCA 
TTCACTGGAA CATCAGGATC ATTAGAGTAC AGTATATCAG AGGAGGATCC TGGGCCATTA 
CGGAGTGACA GTGAAGGCAA AATTGGAGGA ACCAAAGTGA GCAGGACATT CAGTTACCTC 
AAGAGCAAGA TGAGCAAGAA GAACAAGGAG AAGGAGAAAG AGAAGAATAA GGAGCGTGAG 
CGAGATGCCA AAGACAAGGA GAGAAGAGCC TCAAACGGAC ATCTCTTCAC TACGGTCATG 
ACTGTACCCA CAATCCCCTG CCACCAGTGC AACAAACTCA TCAACACTAA AGACTCTATC 
CTCTGCACCA ATTGCAATGC ACAAGTCCAC AAAGGCTGCA AAGAGAGTCT TCCTGTCTGC 
GCTAAAGTCA AGATGAAGCA GCAAAAACAG CAGCAGACTG TTACAGATTC TGCCTCGACG 
CCTAGTGTCA CCCTCAGAAG TAAAACTCTT CCAGCAAGGG ATCGTCCGTG GTCCACAATA 
TCCATTCCCG ATGACCAGCC GGCTCCCCTC GCTCCTCCTC GGAAAAGCCC CAGCAGCATC 
ATGTCTTTCA ACAGCAACCC ACTGTCCAAG AGCATGTCCA TTAATAACAT AGCAGGACAA 
ATGGACGACA CTCCTCTTAA GAAGTTTCTG TCCCAGTCAA CGGACTCTCT TCACAAGACC 
AGTAAAGTCA CGGAGTCTAC AGAATCTCTT ACTGATGAGG GTACAGAGAT GATGGACAGC 
CAGCTGATGG GAGAATTTGA AGCTGACGCC AAAGAGCTGG AGGCTGATTC TTATAGCTTC 
ACGGTAGACA AGAAATTTCT TAAGCAGCTT AAAAAGGATG TTATCAAAAG GCAAGACGTC 
ATCTATGAGC TCATCCAGAC AGAAATGCAC CATGTAAGGA CCTTAAAGAT CATGGCTGAT 
GTTTACAGCA AAGGACTACT GAGAGAAGTT CAACTAGAAG CTCAGACGGT GGAGAAGATG 
TTCCCCATGT TGGACGATGT TCTGGATTTG CACACACAGT TCTTTTCACA CCTGCTTGAA 
AGAAAGAAGG AATCAGGGAG CCAAGAAGAG GGCGGCTTTG TCATCCAAAG GATTGGCGAT 
GTGCTGGTTT CTCAGTTCTC AGGTTCCAGT GCTGAGAGAA TGAAGAAGGT CTATGGGAAG 
TTCTGCGGCA GGCACAATGA AGCTGTGAAC TTCTACAAGG AGCTTCACAC CAAAGACAAA 
CGCTTTCAAG CTTTCATCAA GAAAAAGATG AGCAGCCCTG TTGTCCGACG GCTAAGCATT 
CCTGAGTGTA TATTGTTAGT CACACAACGA ATCACAAAAT ATCCCGTGTT ACTACAGAGA 
ATACTGCAGC ACACGAAAGA AAACGAGGAG GACCATGCAG ACGTTAGTCA AGGTCTGAAG 
CTGGTGAAGG AGGTGATTGC TGCCGTGGAC AATAAAGTGA ATGAGCATGA GAAGAAGAAG 
AGGCTAAAGG AAGTGTACAG CCGGACGGAC AACAAGTCCA TCATGAGGAT GAAGAGCGGG 
CAGATGTTTG CCAGAGAGGA TCTGTTACGT GGGCGGAAGT TAATTCACGA CGGGCCGCTG 
CAGCTCAAGA ACTCAGCTGG CAGACTCAAG GATGTCCAGG CTCTGCTGCT GAGGGACGTG 
ATTGTGTTCC TGCAGGAGAA AGATCAGAAG TACATTTTCG CCTCTTTGGA CCAGCGCTCC 
ACCGTCATCT CCCTGCAGAA GCTCATTGTG AGGGAAGTAG CCAATGAGGA GAAAGCTCTG 
TTTCTGATCA CAGCAGGCAT CGAGAAGCCC GAGATGGTAG AAGTGTACAC AAGCTCCAAA 
GAGGAGAGAA ACACATGGAT GCAGCTCATT CAGGAGGCCA TGCAGTCTAT ACTGTCCATT 
TCCTCCAGGG AGAGAGATGA TGATGAAGGT GTGCCAAGTG AAAACGAAGA GGACAGGAGA 
CTTCAGGAAA TCAAGGTCAA GGAGTTCAGA GATCAGCTGC ACAAAAGAGA CGAGCAGATC 
TTGACGTTAC TGGAGGAGAA GGTGAAGTTG TTCAGGGACA TGTGTGACTG TGGTGCACCG 
GACGAGACGG GGCTTCGTAA TCGAATGCTC TTCAGGGCCA CGACGGATGA TGTCACCAGA 
GGAGAGCCAA TCATGAAGGA GGCTCTGAAG GAAGTTGAAA CGCTGCAGGT TCTGGTTAAT 
GACAGTCTTG GCGGTGCGGT TCAGGAAGGT GTGTGCGCGG CTGGACATGT GACTTTGCCA 
CGACGAGCGG AAACATTTGG AGGTTTCGAC AGTCACCAGA TGAACATCTC TAAGAGCAAA 
GATGGAGACA GAGAGGAGCT GGAGGAGTCT GCAGAGCTCC GCAGGACTGA GTCAGACAGT 
GTGTTGAAGA AGACGAGTAA GACCAACCCT TTACTGCTGG TCAGGAGAAA CAATGAGCAG 
GTTCTTCAGA GCGTTGCTCA TCTTCATGAC CTGCTGAACA CATTACAAGC TGTGGTGGTC 
CAGCAGGACA CCTTCATCGA AGACCAGCGT CACGCTCATC CCTCCTCACG CCACCCATCC 
ATCTCCTCCT CTACCTCCTC TTTATCATCC ACCTCATCCC GGCCGAGCTC TCTAATCGAG 
CAGGAGAAAC AGCGCAGCCT GGAGAAGCAG CGGCAGGAGG TGGCCAGCCT CCAAAAACAG 
CAGGCGGCCC ACGCCGAGGA GAGGCGGCGC AGGGAAAAGC AGTGGGAGAC CCGAGAGAAA 
GAGCTAACCC AGAGGGAGAT GCAGATCCAG AGCCAAGAAG AGGAGGCGCA GAGGAGAGCC 
AGACAGCTGG AGGAGGAGAA ACAGGAGCTG CAGAGTAAGA AAGAGGAGTA TCAGAGAGAC 
CTGGCACGAC TGCGGGACAG CCAGAAGAGA CTGGAGAGAG AGAGAGAGCA GGTGCAGAGA 
GAGGCAGATG AGCTTAAACT GACTGAGGAA GTGACAAAGA ACCGGTCTCC GTCAACCACG 
TCTGAAGACT CCACGAAGTT CCAGAGCACC GGTTCATTAG ACCGTGACCC ATCAGAGATG 
GAGCTCTCCT CCTCCCCGTC AGCCAGAGAC TTCCTGTCCC GCATGGACTC CAAACGCAAA 
GGGAAAAACC TCAATCCATT CTCATCCAAC TCAAGCCAGA AGAGCCAGAA CAGCGAGGCT 
CAGAGCCAGA TGCCCAGCCG TCTGCTCCAG CTGGCCAAAC CCAAGGAGAA GAAGGACAAG 
AAGAAGAAGA AGGGGAAAGG CCAGCAGAGC CAGACTGCAG ACTCTCAGAG CACTGTTGTT 
CCAGGCGCTA CCCCAGACGG AGAGATCTTT TTCTGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

wu:fi15d04 ( XM_021470632.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_021326307.1 Danio rerio wu:fi15d04 (wu:fi15d04), transcript variant X4, mRNA.

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CloneID	ODa141427
Clone ID Related Accession (Same CDS sequence)	XM_021470632.1
Accession Version	XM_021470632.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7659bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	A-kinase anchor protein 13 isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007136.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAACTGA ATCCACAGCA AGCCCCATTA TATGGAGAGT GTCTGCTGAC GGTCCAGCTC 
TGTGAGGATG AGTGTGTTGA GGATGAGGAT GATGTGGAGT TTTACCTGCT CTTTGCTGGA 
TCCACACAGA GACATGTGTC CAGCACACTG AAGATCAGCC ATGTCACGCT GCAGGCCGTC 
TGTCCAGCTC ATGATCGCAG TGAAACGGTA CGGGTCACAC TATGCCAGGC TCGTCCCGGC 
GGCTCAGTGG ACCCAGTGTC CGAGGAGTGT TTCCAGTTTG TCCAGGACCT GGCTCTGGAC 
ATGGCCCAGT TCCTGGTAAA TGCGGCGGGG AAAACAGAAG GAGCGCTGCT GCTGGACGAG 
TGTCAGATCC CACTGAAAGA GTGCGAGCGG CTGGACGATA CTCTTGCTCT GGCTCTCCGG 
CATCTGCCTT TGCCTGCTGG ATGGAGCGTT TTGGGCACAG ATTTGGACAC GGGCACTTGC 
ACAGGCTCGG GCATCGCAGA GCCTGGACCA CACGATACAC TTTTGCACTT CGCTGCGAAG 
CGCGGACTAC GCAAGGTTGC ACTCTTTCTG CTCCAGCAAC CAGGGGGAAG AGAGGCTCTC 
CGGCTTCCCA ACAAACAGGG TCGCACACCG GCCAAGGTCG CCCAGAAGAG GGGACACTCT 
CAGCTCCACA AACTGCTCAC GGAGGTTGAC AATCTGCCCG AGCTGGAGAC GAAAACATCT 
AAGTGGTGTT ACCCAGAAGG CAGAGTCCTG CAACACCACC CCAAGCTAAA CACTTACACC 
CTTACCCTGG AGGATGTGCC CAGGAGCTCC CCACCAAGCC TTCAGTGTGA TGTGGAGGAG 
CTCCAGCGTC TCATTCAGTC ACATCAGAAG GAAGATCCTC CAACACCGCA TAAGTCAGCA 
CTGCTGATTC TCAATAGAGA CTCCAGTAAT AGCACAGAGA CAGATGACTG CCTTCCGCCC 
AACTCAGTGG AACCTGAGCA TCAGGAGCAC AACAAACAAG AAGAAACTTG CAAATCGCTT 
TTAAACAGCC TTACCTTTGA GGATGGATTG TGTGTTCATC AAAACGGCAC AGGAGATCAT 
GTCCAGTTGC TGGATGGGGA CGTGGAGCCT GACCTGCCAG CTGTAGGAGA TACAGGGAAT 
GCATTAGATA AGACTGACTG TTTGCAGCGG CGGGTAGATG GTGATTCTGT GGCTTTCAGT 
GCCCCTTCCT GTGGAAACCA GCGGGAGGAA GCCGATATAG AGCTCTGCAT AATCAGTGCC 
AAGCAGGGGG TTGCTTCACA GCCTGATAAA GGGAAGCAAG AAGAGCAAGA GGCTTGCCAG 
AAGAGGTCAG AGACTGAAAA GGACTATAAC ACTGAATCTG GCAACCTTGG GCTTCAAGAC 
ATGGGCCACG CACAATCTCA GGGTCTCCTC CCCACGGAAA CTGGTCAGCC TACACAAAGG 
GAGGTGGAGA GTGAGGGGAG TACAGATGTC TGTTGGCGTG GGGACTTACC AGAGGAAAGG 
GAAGGGGACA TGAGTAGCAA CCAGGAAATA GACTCATATT CTTCAGCACT TTCTTCCCAT 
AATCTAGAGG GAAACAGCCA GCACGATGGT GAGACTGAGC CCCAGTTGCC TACACAATGC 
TCAGAGGGAA CAGATGAAGC TAAATTGACT TTAATTCAGG TAAATTCTGA AGTTACTTTG 
TCTATACGAA GCGAGGATAC CGTTATTTCT GACAGTGTTG ACAAGCCACA TGGTCTCTTA 
GAGACGGAGA CAGGAAATCT CAGCGAACAC ACCAAAGGCA ACACTGAGTC AGCTGCTGAT 
TTGGTTGCGG ATGACGCTAG TGTAAAGCAG AACTCTGGAT TTAGCGTTTT AGAGGGGGAT 
CTTTTGGAAT CATTAAGTCT ACGGCCAAAC ATGGCACCGG GACCAGTGCA TAAGGTTCAG 
CAAGGTCTGT CACCAGTTCC TGAGACTTCC TCTCATGTAG GTCCAGCTTT GGACCCCTTT 
TCTGAGACTA CAGAAAGTTT TCAACAGCCG AATCAATCCA CACAGCTTTT AGAAACTGCA 
TTAGAATGTG AGGATGTTTC ATTAAATAAC TCAACCGGTG ACATTAATGC AATAGCAGGG 
GTTTCTGTTG ACATTTCTGC AGAGGTTTCA CTTTGCCAGA ATGAAGGGTG TCCTCCTGAT 
GGAAGCAAAG AAGAGGTAGC CCCCAGTGAG AGGATACCAG ACCTTAAAGA GCTTACGGAA 
GAGCCGGAAT CAGTTGAGGA CATTATTGTC TCAGATGTCT CAGATTTGGA TGACAAATCT 
CAACCTTTTG AAGAGTCTTC TTTGCCTATC GACTCTGAAT GTTTTATAAG AGGTGATTTC 
CAAAACGATG AGTCTACAGT ACAGTCTTCT GAATGTACTG GAGGTGACAT AAATGTAGGA 
ACACTCTTAG GAGAAGAAGA GGCAACTGAA AAAATAGAAC AGCCAGACTC TTCACTTAAA 
CTGGTTGAGC TAATTCCTGA GGAATATACT GGAGATGCCA CAAACGAAAA TAAAAAAATT 
GAGCCAGAGG AACAATCTAT TTTACTGCCA ACTTTGCAAG GGGAATCAGG ATTTAAGGAT 
GTTGCCACAG AGGAACCTGT ACAGGAAAGG GAAATTGACT CCACTTCAGA GGAAACAATC 
AGCGATCTCC CTGATCCATC GGAAATCACA AACAATGATT GTGCTGAATC CAATGCACAC 
TGCCAGCTCT CAGAATCCTC CAAGGAGAGC GCAAACACAT CCTCAGAACC TGCTGAATGT 
GTGAATGACC TAGACTTGTC TTGTGCTTTA CCTCAAACAG AAATCGAAGG CAGCAGACCT 
TCTGAGGGCC CCTTAATTGA TGGAGTAACA ATATGTGCTG AAATTCCCAT TGATGACAAA 
GGTGTTGACA TGGCTACCAA TATGAGTAAG ACGACCATCT CTGAGGATTG TCTGTCCTCT 
CCTATGGACA CCACCAGCTG TTCAGTTCTG GAGAGCTCCT CCATGTCTGA CCCACTCAAT 
GCCTCATCCT CAACAGCTGA GCTCACTTCC CCTTTGGATA TTAATAGTGG ATTGCATCAG 
GATTTGGAAC ACACTTCCCC AGTGGATATA GACAATGGTT TGCCTGAGGA ACCGCCGCCG 
TCAGACACAC CTGATTGCCT GAATGGATTA CATCAAGATT CTCCTGATAG TTTGATGGAG 
CACAGTGGAA CTCCTCAGCC ATGTTCTGAG GAACAGTGTG TGGATCAAAT CACAGATGAC 
AGCACTTCAG CAGAGGATGC AGCGGACGGA CACCAAGACT CCTCAGTAGA CATAGCAAAT 
TCCGAAGCGA TCCACAGAGA GACCGAAACA TTTTATTCAG TTGATGCCAG TCAAGACTCT 
GTACTCCAGG AGAAGAAATC TTCTTCAGTG AGCTCAGAAA TCCCCTCTTT GACCCCCTCA 
CCTGAGAGCA TACGTCACAA AGAGTCGCCA AGCACTATGC GAGGGTCCGC CAGCGATGGA 
GACTCTCTGT TTAGTCAGGA TCTGGGCGAG GACAGCGTTT TTAATAATAA GGCTGAGGAC 
AGCATTACCA TCACCAGTGG CGTGTCCATG TCCTATTCGT CCTCCACAGA TGACTCTTCC 
AGCTTGGGAA CCACTTGCGC CACCCCTCAG ACTGGCACAG AGGCTCCTGC ATCAGACCCC 
TTTCAGGAAC AGCCCACATC TACTGGAGCT TCTGGGGAAA CAGAGGAGGA AGAGAAAAAG 
GACCGTCTCA CTGAGGTTCC GGAGCGATCG GCCATTTTAC GGACCACCAT ACGATCACTG 
TCCCCTTTTC GAAGACACAG TTGGGGGCCG GGCAAAAATT TGGCAGGGGA GACAGAGATG 
AGTCAGCGCA GTGCGGCACA AGGTGAAGTG GAGCGCAGAT CTGTTGGTCA TAGGAGAAGC 
ATGAGCTGGT GTCCTTCTGT TGTCCTGCGC TCTGAAAATG ATGAAATGAA TGAAAGAAGT 
TACAGTCTGG AAGGACTTGC ACCAGAGAAG GAGGGAAGGA AAAGTCTGGT TCAGCAGCGT 
GTCGGAGAGT CCAGCCAGGA GCCCAAATGT CCATCAAAGG TGGAAAAAGA AGACAGAGGC 
TCGCTTGTGT CTCTGACTGA AGAAGAGCAG GAGTCAGATC TAGGCGACTG CAGCAGTTTG 
GGCAGTCAGA AGTCCATCCA GTCGGGTCGG AGATGTGTCC CACATTCACA ACCCATCCTC 
ACCAAATCTG TGTCCATGGT GGCCATCAGT CAGAAGGACC TGGACGCAAT GGGACGGCTG 
CGACCCAAGC GACGAATCTC CTTCTCCTTC AGCATCTCGC CGATGCTCCC CAAATCTAAA 
ACTCTCTTTT CTCTTGGCTC TTCATCCAGT GATGAGGAGG AGGCAGTCAG ATTGAGTTCA 
TTCACTGGAA CATCAGGATC ATTAGAGTAC AGTATATCAG AGGAGGATCC TGGGCCATTA 
CGGAGTGACA GTGAAGGCAA AATTGGAGGA ACCAAAGTGA GCAGGACATT CAGTTACCTC 
AAGAGCAAGA TGAGCAAGAA GAACAAGCAG GAGAAGGAGA AAGAGAAGAA TAAGGAGCGT 
GAGCGAGATG CCAAAGACAA GGAGAGAAGA GCCTCAAACG GACATCTCTT CACTACGGTC 
ATGACTGTAC CCACAATCCC CTGCCACCAG TGCAACAAAC TCATCAACAC TAAAGACTCT 
ATCCTCTGCA CCAATTGCAA TGCACAAGTC CACAAAGGCT GCAAAGAGAG TCTTCCTGTC 
TGCGCTAAAG TCAAGATGAA GCAGCAAAAA CAGCAGCAGA CTGTTACAGA TTCTGCCTCG 
ACGCCTAGTG TCACCCTCAG AAGTAAAACT CTTCCAGCAA GGGATCGTCC GTGGTCCACA 
ATATCCATTC CCGATGACCA GCCGGCTCCC CTCGCTCCTC CTCGGAAAAG CCCCAGCAGC 
ATCATGTCTT TCAACAGCAA CCCACTGTCC AAGAGCATGT CCATTAATAA CATAGCAGGA 
CAAATGGACG ACACTCCTCT TAAGAAGTTT CTGTCCCAGT CAACGGACTC TCTTCACAAG 
ACCAGTAAAG TCACGGAGTC TACAGAATCT CTTACTGATG AGGGTACAGA GATGATGGAC 
AGCCAGCTGA TGGGAGAATT TGAAGCTGAC GCCAAAGAGC TGGAGGCTGA TTCTTATAGC 
TTCACGGTAG ACAAGAAATT TCTTAAGCAG CTTAAAAAGG ATGTTATCAA AAGGCAAGAC 
GTCATCTATG AGCTCATCCA GACAGAAATG CACCATGTAA GGACCTTAAA GATCATGGCT 
GATGTTTACA GCAAAGGACT ACTGAGAGAA GTTCAACTAG AAGCTCAGAC GGTGGAGAAG 
ATGTTCCCCA TGTTGGACGA TGTTCTGGAT TTGCACACAC AGTTCTTTTC ACACCTGCTT 
GAAAGAAAGA AGGAATCAGG GAGCCAAGAA GAGGGCGGCT TTGTCATCCA AAGGATTGGC 
GATGTGCTGG TTTCTCAGTT CTCAGGTTCC AGTGCTGAGA GAATGAAGAA GGTCTATGGG 
AAGTTCTGCG GCAGGCACAA TGAAGCTGTG AACTTCTACA AGGAGCTTCA CACCAAAGAC 
AAACGCTTTC AAGCTTTCAT CAAGAAAAAG ATGAGCAGCC CTGTTGTCCG ACGGCTAAGC 
ATTCCTGAGT GTATATTGTT AGTCACACAA CGAATCACAA AATATCCCGT GTTACTACAG 
AGAATACTGC AGCACACGAA AGAAAACGAG GAGGACCATG CAGACGTTAG TCAAGGTCTG 
AAGCTGGTGA AGGAGGTGAT TGCTGCCGTG GACAATAAAG TGAATGAGCA TGAGAAGAAG 
AAGAGGCTAA AGGAAGTGTA CAGCCGGACG GACAACAAGT CCATCATGAG GATGAAGAGC 
GGGCAGATGT TTGCCAGAGA GGATCTGTTA CGTGGGCGGA AGTTAATTCA CGACGGGCCG 
CTGCAGCTCA AGAACTCAGC TGGCAGACTC AAGGATGTCC AGGCTCTGCT GCTGAGGGAC 
GTGATTGTGT TCCTGCAGGA GAAAGATCAG AAGTACATTT TCGCCTCTTT GGACCAGCGC 
TCCACCGTCA TCTCCCTGCA GAAGCTCATT GTGAGGGAAG TAGCCAATGA GGAGAAAGCT 
CTGTTTCTGA TCACAGCAGG CATCGAGAAG CCCGAGATGG TAGAAGTGTA CACAAGCTCC 
AAAGAGGAGA GAAACACATG GATGCAGCTC ATTCAGGAGG CCATGCAGTC TATACTGTCC 
ATTTCCTCCA GGGAGAGAGA TGATGATGAA GGTGTGCCAA GTGAAAACGA AGAGGACAGG 
AGACTTCAGG AAATCAAGGT CAAGGAGTTC AGAGATCAGC TGCACAAAAG AGACGAGCAG 
ATCTTGACGT TACTGGAGGA GAAGGTGAAG TTGTTCAGGG ACATGTGTGA CTGTGGTGCA 
CCGGACGAGA CGGGGCTTCG TAATCGAATG CTCTTCAGGG CCACGACGGA TGATGTCACC 
AGAGGAGAGC CAATCATGAA GGAGGCTCTG AAGGAAGTTG AAACGCTGCA GGTTCTGGTT 
AATGACAGTC TTGGCGGTGC GGTTCAGGAA GGTGTGTGCG CGGCTGGACA TGTGACTTTG 
CCACGACGAG CGGAAACATT TGGAGGTTTC GACAGTCACC AGATGAACAT CTCTAAGAGC 
AAAGATGGAG ACAGAGAGGA GCTGGAGGAG TCTGCAGAGC TCCGCAGGAC TGAGTCAGAC 
AGTGTGTTGA AGAAGACGAG TAAGACCAAC CCTTTACTGC TGGTCAGGAG AAACAATGAG 
GTTCTTCAGA GCGTTGCTCA TCTTCATGAC CTGCTGAACA CATTACAAGC TGTGGTGGTC 
CAGCAGGACA CCTTCATCGA AGACCAGCGT CACGCTCATC CCTCCTCACG CCACCCATCC 
ATCTCCTCCT CTACCTCCTC TTTATCATCC ACCTCATCCC GGCCGAGCTC TCTAATCGAG 
CAGGAGAAAC AGCGCAGCCT GGAGAAGCAG CGGCAGGAGG TGGCCAGCCT CCAAAAACAG 
CAGGCGGCCC ACGCCGAGGA GAGGCGGCGC AGGGAAAAGC AGTGGGAGAC CCGAGAGAAA 
GAGCTAACCC AGAGGGAGAT GCAGATCCAG AGCCAAGAAG AGGAGGCGCA GAGGAGAGCC 
AGACAGCTGG AGGAGGAGAA ACAGGAGCTG CAGAGTAAGA AAGAGGAGTA TCAGAGAGAC 
CTGGCACGAC TGCGGGACAG CCAGAAGAGA CTGGAGAGAG AGAGAGAGCA GGTGCAGAGA 
GAGGCAGATG AGCTTAAACT GACTGAGGAA GTGACAAAGA ACCGGTCTCC GTCAACCACG 
TCTGAAGACT CCACGAAGTT CCAGAGCACC GGTTCATTAG ACCGTGACCC ATCAGAGATG 
GAGCTCTCCT CCTCCCCGTC AGCCAGAGAC TTCCTGTCCC GCATGGACTC CAAACGCAAA 
GGGAAAAACC TCAATCCATT CTCATCCAAC TCAAGCCAGA AGAGCCAGAA CAGCGAGGCT 
CAGAGCCAGA TGCCCAGCCG TCTGCTCCAG CTGGCCAAAC CCAAGGAGAA GAAGGACAAG 
AAGAAGAAGA AGGGGAAAGG CCAGCAGAGC CAGACTGCAG ACTCTCAGAG CACTGTTGTT 
CCAGGCGCTA CCCCAGACGG AGAGATCTTT TTCTGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_021326307.1
CDS	398..8056
Translation	MKLNPQQAPLYGECLLTVQLCEDECVEDEDDVEFYLLFAGSTQRHVSSTLKISHVTLQAVCPAHDRSETVRVTLCQARPGGSVDPVSEECFQFVQDLALDMAQFLVNAAGKTEGALLLDECQIPLKECERLDDTLALALRHLPLPAGWSVLGTDLDTGTCTGSGIAEPGPHDTLLHFAAKRGLRKVALFLLQQPGGREALRLPNKQGRTPAKVAQKRGHSQLHKLLTEVDNLPELETKTSKWCYPEGRVLQHHPKLNTYTLTLEDVPRSSPPSLQCDVEELQRLIQSHQKEDPPTPHKSALLILNRDSSNSTETDDCLPPNSVEPEHQEHNKQEETCKSLLNSLTFEDGLCVHQNGTGDHVQLLDGDVEPDLPAVGDTGNALDKTDCLQRRVDGDSVAFSAPSCGNQREEADIELCIISAKQGVASQPDKGKQEEQEACQKRSETEKDYNTESGNLGLQDMGHAQSQGLLPTETGQPTQREVESEGSTDVCWRGDLPEEREGDMSSNQEIDSYSSALSSHNLEGNSQHDGETEPQLPTQCSEGTDEAKLTLIQVNSEVTLSIRSEDTVISDSVDKPHGLLETETGNLSEHTKGNTESAADLVADDASVKQNSGFSVLEGDLLESLSLRPNMAPGPVHKVQQGLSPVPETSSHVGPALDPFSETTESFQQPNQSTQLLETALECEDVSLNNSTGDINAIAGVSVDISAEVSLCQNEGCPPDGSKEEVAPSERIPDLKELTEEPESVEDIIVSDVSDLDDKSQPFEESSLPIDSECFIRGDFQNDESTVQSSECTGGDINVGTLLGEEEATEKIEQPDSSLKLVELIPEEYTGDATNENKKIEPEEQSILLPTLQGESGFKDVATEEPVQEREIDSTSEETISDLPDPSEITNNDCAESNAHCQLSESSKESANTSSEPAECVNDLDLSCALPQTEIEGSRPSEGPLIDGVTICAEIPIDDKGVDMATNMSKTTISEDCLSSPMDTTSCSVLESSSMSDPLNASSSTAELTSPLDINSGLHQDLEHTSPVDIDNGLPEEPPPSDTPDCLNGLHQDSPDSLMEHSGTPQPCSEEQCVDQITDDSTSAEDAADGHQDSSVDIANSEAIHRETETFYSVDASQDSVLQEKKSSSVSSEIPSLTPSPESIRHKESPSTMRGSASDGDSLFSQDLGEDSVFNNKAEDSITITSGVSMSYSSSTDDSSSLGTTCATPQTGTEAPASDPFQEQPTSTGASGETEEEEKKDRLTEVPERSAILRTTIRSLSPFRRHSWGPGKNLAGETEMSQRSAAQGEVERRSVGHRRSMSWCPSVVLRSENDEMNERSYSLEGLAPEKEGRKSLVQQRVGESSQEPKCPSKVEKEDRGSLVSLTEEEQESDLGDCSSLGSQKSIQSGRRCVPHSQPILTKSVSMVAISQKDLDAMGRLRPKRRISFSFSISPMLPKSKTLFSLGSSSSDEEEAVRLSSFTGTSGSLEYSISEEDPGPLRSDSEGKIGGTKVSRTFSYLKSKMSKKNKQEKEKEKNKERERDAKDKERRASNGHLFTTVMTVPTIPCHQCNKLINTKDSILCTNCNAQVHKGCKESLPVCAKVKMKQQKQQQTVTDSASTPSVTLRSKTLPARDRPWSTISIPDDQPAPLAPPRKSPSSIMSFNSNPLSKSMSINNIAGQMDDTPLKKFLSQSTDSLHKTSKVTESTESLTDEGTEMMDSQLMGEFEADAKELEADSYSFTVDKKFLKQLKKDVIKRQDVIYELIQTEMHHVRTLKIMADVYSKGLLREVQLEAQTVEKMFPMLDDVLDLHTQFFSHLLERKKESGSQEEGGFVIQRIGDVLVSQFSGSSAERMKKVYGKFCGRHNEAVNFYKELHTKDKRFQAFIKKKMSSPVVRRLSIPECILLVTQRITKYPVLLQRILQHTKENEEDHADVSQGLKLVKEVIAAVDNKVNEHEKKKRLKEVYSRTDNKSIMRMKSGQMFAREDLLRGRKLIHDGPLQLKNSAGRLKDVQALLLRDVIVFLQEKDQKYIFASLDQRSTVISLQKLIVREVANEEKALFLITAGIEKPEMVEVYTSSKEERNTWMQLIQEAMQSILSISSRERDDDEGVPSENEEDRRLQEIKVKEFRDQLHKRDEQILTLLEEKVKLFRDMCDCGAPDETGLRNRMLFRATTDDVTRGEPIMKEALKEVETLQVLVNDSLGGAVQEGVCAAGHVTLPRRAETFGGFDSHQMNISKSKDGDREELEESAELRRTESDSVLKKTSKTNPLLLVRRNNEVLQSVAHLHDLLNTLQAVVVQQDTFIEDQRHAHPSSRHPSISSSTSSLSSTSSRPSSLIEQEKQRSLEKQRQEVASLQKQQAAHAEERRRREKQWETREKELTQREMQIQSQEEEAQRRARQLEEEKQELQSKKEEYQRDLARLRDSQKRLEREREQVQREADELKLTEEVTKNRSPSTTSEDSTKFQSTGSLDRDPSEMELSSSPSARDFLSRMDSKRKGKNLNPFSSNSSQKSQNSEAQSQMPSRLLQLAKPKEKKDKKKKKGKGQQSQTADSQSTVVPGATPDGEIFFC

Target ORF information:

RefSeq Version	XM_021470632.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio wu:fi15d04 (wu:fi15d04), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_021470632.1

ORF Insert Sequence:

ATGAAACTGA ATCCACAGCA AGCCCCATTA TATGGAGAGT GTCTGCTGAC GGTCCAGCTC 
TGTGAGGATG AGTGTGTTGA GGATGAGGAT GATGTGGAGT TTTACCTGCT CTTTGCTGGA 
TCCACACAGA GACATGTGTC CAGCACACTG AAGATCAGCC ATGTCACGCT GCAGGCCGTC 
TGTCCAGCTC ATGATCGCAG TGAAACGGTA CGGGTCACAC TATGCCAGGC TCGTCCCGGC 
GGCTCAGTGG ACCCAGTGTC CGAGGAGTGT TTCCAGTTTG TCCAGGACCT GGCTCTGGAC 
ATGGCCCAGT TCCTGGTAAA TGCGGCGGGG AAAACAGAAG GAGCGCTGCT GCTGGACGAG 
TGTCAGATCC CACTGAAAGA GTGCGAGCGG CTGGACGATA CTCTTGCTCT GGCTCTCCGG 
CATCTGCCTT TGCCTGCTGG ATGGAGCGTT TTGGGCACAG ATTTGGACAC GGGCACTTGC 
ACAGGCTCGG GCATCGCAGA GCCTGGACCA CACGATACAC TTTTGCACTT CGCTGCGAAG 
CGCGGACTAC GCAAGGTTGC ACTCTTTCTG CTCCAGCAAC CAGGGGGAAG AGAGGCTCTC 
CGGCTTCCCA ACAAACAGGG TCGCACACCG GCCAAGGTCG CCCAGAAGAG GGGACACTCT 
CAGCTCCACA AACTGCTCAC GGAGGTTGAC AATCTGCCCG AGCTGGAGAC GAAAACATCT 
AAGTGGTGTT ACCCAGAAGG CAGAGTCCTG CAACACCACC CCAAGCTAAA CACTTACACC 
CTTACCCTGG AGGATGTGCC CAGGAGCTCC CCACCAAGCC TTCAGTGTGA TGTGGAGGAG 
CTCCAGCGTC TCATTCAGTC ACATCAGAAG GAAGATCCTC CAACACCGCA TAAGTCAGCA 
CTGCTGATTC TCAATAGAGA CTCCAGTAAT AGCACAGAGA CAGATGACTG CCTTCCGCCC 
AACTCAGTGG AACCTGAGCA TCAGGAGCAC AACAAACAAG AAGAAACTTG CAAATCGCTT 
TTAAACAGCC TTACCTTTGA GGATGGATTG TGTGTTCATC AAAACGGCAC AGGAGATCAT 
GTCCAGTTGC TGGATGGGGA CGTGGAGCCT GACCTGCCAG CTGTAGGAGA TACAGGGAAT 
GCATTAGATA AGACTGACTG TTTGCAGCGG CGGGTAGATG GTGATTCTGT GGCTTTCAGT 
GCCCCTTCCT GTGGAAACCA GCGGGAGGAA GCCGATATAG AGCTCTGCAT AATCAGTGCC 
AAGCAGGGGG TTGCTTCACA GCCTGATAAA GGGAAGCAAG AAGAGCAAGA GGCTTGCCAG 
AAGAGGTCAG AGACTGAAAA GGACTATAAC ACTGAATCTG GCAACCTTGG GCTTCAAGAC 
ATGGGCCACG CACAATCTCA GGGTCTCCTC CCCACGGAAA CTGGTCAGCC TACACAAAGG 
GAGGTGGAGA GTGAGGGGAG TACAGATGTC TGTTGGCGTG GGGACTTACC AGAGGAAAGG 
GAAGGGGACA TGAGTAGCAA CCAGGAAATA GACTCATATT CTTCAGCACT TTCTTCCCAT 
AATCTAGAGG GAAACAGCCA GCACGATGGT GAGACTGAGC CCCAGTTGCC TACACAATGC 
TCAGAGGGAA CAGATGAAGC TAAATTGACT TTAATTCAGG TAAATTCTGA AGTTACTTTG 
TCTATACGAA GCGAGGATAC CGTTATTTCT GACAGTGTTG ACAAGCCACA TGGTCTCTTA 
GAGACGGAGA CAGGAAATCT CAGCGAACAC ACCAAAGGCA ACACTGAGTC AGCTGCTGAT 
TTGGTTGCGG ATGACGCTAG TGTAAAGCAG AACTCTGGAT TTAGCGTTTT AGAGGGGGAT 
CTTTTGGAAT CATTAAGTCT ACGGCCAAAC ATGGCACCGG GACCAGTGCA TAAGGTTCAG 
CAAGGTCTGT CACCAGTTCC TGAGACTTCC TCTCATGTAG GTCCAGCTTT GGACCCCTTT 
TCTGAGACTA CAGAAAGTTT TCAACAGCCG AATCAATCCA CACAGCTTTT AGAAACTGCA 
TTAGAATGTG AGGATGTTTC ATTAAATAAC TCAACCGGTG ACATTAATGC AATAGCAGGG 
GTTTCTGTTG ACATTTCTGC AGAGGTTTCA CTTTGCCAGA ATGAAGGGTG TCCTCCTGAT 
GGAAGCAAAG AAGAGGTAGC CCCCAGTGAG AGGATACCAG ACCTTAAAGA GCTTACGGAA 
GAGCCGGAAT CAGTTGAGGA CATTATTGTC TCAGATGTCT CAGATTTGGA TGACAAATCT 
CAACCTTTTG AAGAGTCTTC TTTGCCTATC GACTCTGAAT GTTTTATAAG AGGTGATTTC 
CAAAACGATG AGTCTACAGT ACAGTCTTCT GAATGTACTG GAGGTGACAT AAATGTAGGA 
ACACTCTTAG GAGAAGAAGA GGCAACTGAA AAAATAGAAC AGCCAGACTC TTCACTTAAA 
CTGGTTGAGC TAATTCCTGA GGAATATACT GGAGATGCCA CAAACGAAAA TAAAAAAATT 
GAGCCAGAGG AACAATCTAT TTTACTGCCA ACTTTGCAAG GGGAATCAGG ATTTAAGGAT 
GTTGCCACAG AGGAACCTGT ACAGGAAAGG GAAATTGACT CCACTTCAGA GGAAACAATC 
AGCGATCTCC CTGATCCATC GGAAATCACA AACAATGATT GTGCTGAATC CAATGCACAC 
TGCCAGCTCT CAGAATCCTC CAAGGAGAGC GCAAACACAT CCTCAGAACC TGCTGAATGT 
GTGAATGACC TAGACTTGTC TTGTGCTTTA CCTCAAACAG AAATCGAAGG CAGCAGACCT 
TCTGAGGGCC CCTTAATTGA TGGAGTAACA ATATGTGCTG AAATTCCCAT TGATGACAAA 
GGTGTTGACA TGGCTACCAA TATGAGTAAG ACGACCATCT CTGAGGATTG TCTGTCCTCT 
CCTATGGACA CCACCAGCTG TTCAGTTCTG GAGAGCTCCT CCATGTCTGA CCCACTCAAT 
GCCTCATCCT CAACAGCTGA GCTCACTTCC CCTTTGGATA TTAATAGTGG ATTGCATCAG 
GATTTGGAAC ACACTTCCCC AGTGGATATA GACAATGGTT TGCCTGAGGA ACCGCCGCCG 
TCAGACACAC CTGATTGCCT GAATGGATTA CATCAAGATT CTCCTGATAG TTTGATGGAG 
CACAGTGGAA CTCCTCAGCC ATGTTCTGAG GAACAGTGTG TGGATCAAAT CACAGATGAC 
AGCACTTCAG CAGAGGATGC AGCGGACGGA CACCAAGACT CCTCAGTAGA CATAGCAAAT 
TCCGAAGCGA TCCACAGAGA GACCGAAACA TTTTATTCAG TTGATGCCAG TCAAGACTCT 
GTACTCCAGG AGAAGAAATC TTCTTCAGTG AGCTCAGAAA TCCCCTCTTT GACCCCCTCA 
CCTGAGAGCA TACGTCACAA AGAGTCGCCA AGCACTATGC GAGGGTCCGC CAGCGATGGA 
GACTCTCTGT TTAGTCAGGA TCTGGGCGAG GACAGCGTTT TTAATAATAA GGCTGAGGAC 
AGCATTACCA TCACCAGTGG CGTGTCCATG TCCTATTCGT CCTCCACAGA TGACTCTTCC 
AGCTTGGGAA CCACTTGCGC CACCCCTCAG ACTGGCACAG AGGCTCCTGC ATCAGACCCC 
TTTCAGGAAC AGCCCACATC TACTGGAGCT TCTGGGGAAA CAGAGGAGGA AGAGAAAAAG 
GACCGTCTCA CTGAGGTTCC GGAGCGATCG GCCATTTTAC GGACCACCAT ACGATCACTG 
TCCCCTTTTC GAAGACACAG TTGGGGGCCG GGCAAAAATT TGGCAGGGGA GACAGAGATG 
AGTCAGCGCA GTGCGGCACA AGGTGAAGTG GAGCGCAGAT CTGTTGGTCA TAGGAGAAGC 
ATGAGCTGGT GTCCTTCTGT TGTCCTGCGC TCTGAAAATG ATGAAATGAA TGAAAGAAGT 
TACAGTCTGG AAGGACTTGC ACCAGAGAAG GAGGGAAGGA AAAGTCTGGT TCAGCAGCGT 
GTCGGAGAGT CCAGCCAGGA GCCCAAATGT CCATCAAAGG TGGAAAAAGA AGACAGAGGC 
TCGCTTGTGT CTCTGACTGA AGAAGAGCAG GAGTCAGATC TAGGCGACTG CAGCAGTTTG 
GGCAGTCAGA AGTCCATCCA GTCGGGTCGG AGATGTGTCC CACATTCACA ACCCATCCTC 
ACCAAATCTG TGTCCATGGT GGCCATCAGT CAGAAGGACC TGGACGCAAT GGGACGGCTG 
CGACCCAAGC GACGAATCTC CTTCTCCTTC AGCATCTCGC CGATGCTCCC CAAATCTAAA 
ACTCTCTTTT CTCTTGGCTC TTCATCCAGT GATGAGGAGG AGGCAGTCAG ATTGAGTTCA 
TTCACTGGAA CATCAGGATC ATTAGAGTAC AGTATATCAG AGGAGGATCC TGGGCCATTA 
CGGAGTGACA GTGAAGGCAA AATTGGAGGA ACCAAAGTGA GCAGGACATT CAGTTACCTC 
AAGAGCAAGA TGAGCAAGAA GAACAAGCAG GAGAAGGAGA AAGAGAAGAA TAAGGAGCGT 
GAGCGAGATG CCAAAGACAA GGAGAGAAGA GCCTCAAACG GACATCTCTT CACTACGGTC 
ATGACTGTAC CCACAATCCC CTGCCACCAG TGCAACAAAC TCATCAACAC TAAAGACTCT 
ATCCTCTGCA CCAATTGCAA TGCACAAGTC CACAAAGGCT GCAAAGAGAG TCTTCCTGTC 
TGCGCTAAAG TCAAGATGAA GCAGCAAAAA CAGCAGCAGA CTGTTACAGA TTCTGCCTCG 
ACGCCTAGTG TCACCCTCAG AAGTAAAACT CTTCCAGCAA GGGATCGTCC GTGGTCCACA 
ATATCCATTC CCGATGACCA GCCGGCTCCC CTCGCTCCTC CTCGGAAAAG CCCCAGCAGC 
ATCATGTCTT TCAACAGCAA CCCACTGTCC AAGAGCATGT CCATTAATAA CATAGCAGGA 
CAAATGGACG ACACTCCTCT TAAGAAGTTT CTGTCCCAGT CAACGGACTC TCTTCACAAG 
ACCAGTAAAG TCACGGAGTC TACAGAATCT CTTACTGATG AGGGTACAGA GATGATGGAC 
AGCCAGCTGA TGGGAGAATT TGAAGCTGAC GCCAAAGAGC TGGAGGCTGA TTCTTATAGC 
TTCACGGTAG ACAAGAAATT TCTTAAGCAG CTTAAAAAGG ATGTTATCAA AAGGCAAGAC 
GTCATCTATG AGCTCATCCA GACAGAAATG CACCATGTAA GGACCTTAAA GATCATGGCT 
GATGTTTACA GCAAAGGACT ACTGAGAGAA GTTCAACTAG AAGCTCAGAC GGTGGAGAAG 
ATGTTCCCCA TGTTGGACGA TGTTCTGGAT TTGCACACAC AGTTCTTTTC ACACCTGCTT 
GAAAGAAAGA AGGAATCAGG GAGCCAAGAA GAGGGCGGCT TTGTCATCCA AAGGATTGGC 
GATGTGCTGG TTTCTCAGTT CTCAGGTTCC AGTGCTGAGA GAATGAAGAA GGTCTATGGG 
AAGTTCTGCG GCAGGCACAA TGAAGCTGTG AACTTCTACA AGGAGCTTCA CACCAAAGAC 
AAACGCTTTC AAGCTTTCAT CAAGAAAAAG ATGAGCAGCC CTGTTGTCCG ACGGCTAAGC 
ATTCCTGAGT GTATATTGTT AGTCACACAA CGAATCACAA AATATCCCGT GTTACTACAG 
AGAATACTGC AGCACACGAA AGAAAACGAG GAGGACCATG CAGACGTTAG TCAAGGTCTG 
AAGCTGGTGA AGGAGGTGAT TGCTGCCGTG GACAATAAAG TGAATGAGCA TGAGAAGAAG 
AAGAGGCTAA AGGAAGTGTA CAGCCGGACG GACAACAAGT CCATCATGAG GATGAAGAGC 
GGGCAGATGT TTGCCAGAGA GGATCTGTTA CGTGGGCGGA AGTTAATTCA CGACGGGCCG 
CTGCAGCTCA AGAACTCAGC TGGCAGACTC AAGGATGTCC AGGCTCTGCT GCTGAGGGAC 
GTGATTGTGT TCCTGCAGGA GAAAGATCAG AAGTACATTT TCGCCTCTTT GGACCAGCGC 
TCCACCGTCA TCTCCCTGCA GAAGCTCATT GTGAGGGAAG TAGCCAATGA GGAGAAAGCT 
CTGTTTCTGA TCACAGCAGG CATCGAGAAG CCCGAGATGG TAGAAGTGTA CACAAGCTCC 
AAAGAGGAGA GAAACACATG GATGCAGCTC ATTCAGGAGG CCATGCAGTC TATACTGTCC 
ATTTCCTCCA GGGAGAGAGA TGATGATGAA GGTGTGCCAA GTGAAAACGA AGAGGACAGG 
AGACTTCAGG AAATCAAGGT CAAGGAGTTC AGAGATCAGC TGCACAAAAG AGACGAGCAG 
ATCTTGACGT TACTGGAGGA GAAGGTGAAG TTGTTCAGGG ACATGTGTGA CTGTGGTGCA 
CCGGACGAGA CGGGGCTTCG TAATCGAATG CTCTTCAGGG CCACGACGGA TGATGTCACC 
AGAGGAGAGC CAATCATGAA GGAGGCTCTG AAGGAAGTTG AAACGCTGCA GGTTCTGGTT 
AATGACAGTC TTGGCGGTGC GGTTCAGGAA GGTGTGTGCG CGGCTGGACA TGTGACTTTG 
CCACGACGAG CGGAAACATT TGGAGGTTTC GACAGTCACC AGATGAACAT CTCTAAGAGC 
AAAGATGGAG ACAGAGAGGA GCTGGAGGAG TCTGCAGAGC TCCGCAGGAC TGAGTCAGAC 
AGTGTGTTGA AGAAGACGAG TAAGACCAAC CCTTTACTGC TGGTCAGGAG AAACAATGAG 
GTTCTTCAGA GCGTTGCTCA TCTTCATGAC CTGCTGAACA CATTACAAGC TGTGGTGGTC 
CAGCAGGACA CCTTCATCGA AGACCAGCGT CACGCTCATC CCTCCTCACG CCACCCATCC 
ATCTCCTCCT CTACCTCCTC TTTATCATCC ACCTCATCCC GGCCGAGCTC TCTAATCGAG 
CAGGAGAAAC AGCGCAGCCT GGAGAAGCAG CGGCAGGAGG TGGCCAGCCT CCAAAAACAG 
CAGGCGGCCC ACGCCGAGGA GAGGCGGCGC AGGGAAAAGC AGTGGGAGAC CCGAGAGAAA 
GAGCTAACCC AGAGGGAGAT GCAGATCCAG AGCCAAGAAG AGGAGGCGCA GAGGAGAGCC 
AGACAGCTGG AGGAGGAGAA ACAGGAGCTG CAGAGTAAGA AAGAGGAGTA TCAGAGAGAC 
CTGGCACGAC TGCGGGACAG CCAGAAGAGA CTGGAGAGAG AGAGAGAGCA GGTGCAGAGA 
GAGGCAGATG AGCTTAAACT GACTGAGGAA GTGACAAAGA ACCGGTCTCC GTCAACCACG 
TCTGAAGACT CCACGAAGTT CCAGAGCACC GGTTCATTAG ACCGTGACCC ATCAGAGATG 
GAGCTCTCCT CCTCCCCGTC AGCCAGAGAC TTCCTGTCCC GCATGGACTC CAAACGCAAA 
GGGAAAAACC TCAATCCATT CTCATCCAAC TCAAGCCAGA AGAGCCAGAA CAGCGAGGCT 
CAGAGCCAGA TGCCCAGCCG TCTGCTCCAG CTGGCCAAAC CCAAGGAGAA GAAGGACAAG 
AAGAAGAAGA AGGGGAAAGG CCAGCAGAGC CAGACTGCAG ACTCTCAGAG CACTGTTGTT 
CCAGGCGCTA CCCCAGACGG AGAGATCTTT TTCTGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

wu:fi15d04 ( XM_021470633.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_021326308.1 Danio rerio wu:fi15d04 (wu:fi15d04), transcript variant X5, mRNA.

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CloneID	ODa141428
Clone ID Related Accession (Same CDS sequence)	XM_021470633.1
Accession Version	XM_021470633.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7659bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	A-kinase anchor protein 13 isoform X5
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007136.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAACTGA ATCCACAGCA AGCCCCATTA TATGGAGAGT GTCTGCTGAC GGTCCAGCTC 
TGTGAGGATG AGTGTGTTGA GGATGAGGAT GATGTGGAGT TTTACCTGCT CTTTGCTGGA 
TCCACACAGA GACATGTGTC CAGCACACTG AAGATCAGCC ATGTCACGCT GCAGGCCGTC 
TGTCCAGCTC ATGATCGCAG TGAAACGGTA CGGGTCACAC TATGCCAGGC TCGTCCCGGC 
GGCTCAGTGG ACCCAGTGTC CGAGGAGTGT TTCCAGTTTG TCCAGGACCT GGCTCTGGAC 
ATGGCCCAGT TCCTGGTAAA TGCGGCGGGG AAAACAGAAG GAGCGCTGCT GCTGGACGAG 
TGTCAGATCC CACTGAAAGA GTGCGAGCGG CTGGACGATA CTCTTGCTCT GGCTCTCCGG 
CATCTGCCTT TGCCTGCTGG ATGGAGCGTT TTGGGCACAG ATTTGGACAC GGGCACTTGC 
ACAGGCTCGG GCATCGCAGA GCCTGGACCA CACGATACAC TTTTGCACTT CGCTGCGAAG 
CGCGGACTAC GCAAGGTTGC ACTCTTTCTG CTCCAGCAAC CAGGGGGAAG AGAGGCTCTC 
CGGCTTCCCA ACAAACAGGG TCGCACACCG GCCAAGGTCG CCCAGAAGAG GGGACACTCT 
CAGCTCCACA AACTGCTCAC GGAGGTTGAC AATCTGCCCG AGCTGGAGAC GAAAACATCT 
AAGTGGTGTT ACCCAGAAGG CAGAGTCCTG CAACACCACC CCAAGCTAAA CACTTACACC 
CTTACCCTGG AGGATGTGCC CAGGAGCTCC CCACCAAGCC TTCAGTGTGA TGTGGAGGAG 
CTCCAGCGTC TCATTCAGTC ACATCAGAAG GAAGATCCTC CAACACCGCA TAAGTCAGCA 
CTGCTGATTC TCAATAGAGA CTCCAGTAAT AGCACAGAGA CAGATGACTG CCTTCCGCCC 
AACTCAGTGG AACCTGAGCA TCAGGAGCAC AACAAACAAG AAGAAACTTG CAAATCGCTT 
TTAAACAGCC TTACCTTTGA GGATGGATTG TGTGTTCATC AAAACGGCAC AGGAGATCAT 
GTCCAGTTGC TGGATGGGGA CGTGGAGCCT GACCTGCCAG CTGTAGGAGA TACAGGGAAT 
GCATTAGATA AGACTGACTG TTTGCAGCGG CGGGTAGATG GTGATTCTGT GGCTTTCAGT 
GCCCCTTCCT GTGGAAACCA GCGGGAGGAA GCCGATATAG AGCTCTGCAT AATCAGTGCC 
AAGCAGGGGG TTGCTTCACA GCCTGATAAA GGGAAGCAAG AAGAGCAAGA GGCTTGCCAG 
AAGAGGTCAG AGACTGAAAA GGACTATAAC ACTGAATCTG GCAACCTTGG GCTTCAAGAC 
ATGGGCCACG CACAATCTCA GGGTCTCCTC CCCACGGAAA CTGGTCAGCC TACACAAAGG 
GAGGTGGAGA GTGAGGGGAG TACAGATGTC TGTTGGCGTG GGGACTTACC AGAGGAAAGG 
GAAGGGGACA TGAGTAGCAA CCAGGAAATA GACTCATATT CTTCAGCACT TTCTTCCCAT 
AATCTAGAGG GAAACAGCCA GCACGATGGT GAGACTGAGC CCCAGTTGCC TACACAATGC 
TCAGAGGGAA CAGATGAAGC TAAATTGACT TTAATTCAGG TAAATTCTGA AGTTACTTTG 
TCTATACGAA GCGAGGATAC CGTTATTTCT GACAGTGTTG ACAAGCCACA TGGTCTCTTA 
GAGACGGAGA CAGGAAATCT CAGCGAACAC ACCAAAGGCA ACACTGAGTC AGCTGCTGAT 
TTGGTTGCGG ATGACGCTAG TGTAAAGCAG AACTCTGGAT TTAGCGTTTT AGAGGGGGAT 
CTTTTGGAAT CATTAAGTCT ACGGCCAAAC ATGGCACCGG GACCAGTGCA TAAGGTTCAG 
CAAGGTCTGT CACCAGTTCC TGAGACTTCC TCTCATGTAG GTCCAGCTTT GGACCCCTTT 
TCTGAGACTA CAGAAAGTTT TCAACAGCCG AATCAATCCA CACAGCTTTT AGAAACTGCA 
TTAGAATGTG AGGATGTTTC ATTAAATAAC TCAACCGGTG ACATTAATGC AATAGCAGGG 
GTTTCTGTTG ACATTTCTGC AGAGGTTTCA CTTTGCCAGA ATGAAGGGTG TCCTCCTGAT 
GGAAGCAAAG AAGAGGTAGC CCCCAGTGAG AGGATACCAG ACCTTAAAGA GCTTACGGAA 
GAGCCGGAAT CAGTTGAGGA CATTATTGTC TCAGATGTCT CAGATTTGGA TGACAAATCT 
CAACCTTTTG AAGAGTCTTC TTTGCCTATC GACTCTGAAT GTTTTATAAG AGGTGATTTC 
CAAAACGATG AGTCTACAGT ACAGTCTTCT GAATGTACTG GAGGTGACAT AAATGTAGGA 
ACACTCTTAG GAGAAGAAGA GGCAACTGAA AAAATAGAAC AGCCAGACTC TTCACTTAAA 
CTGGTTGAGC TAATTCCTGA GGAATATACT GGAGATGCCA CAAACGAAAA TAAAAAAATT 
GAGCCAGAGG AACAATCTAT TTTACTGCCA ACTTTGCAAG GGGAATCAGG ATTTAAGGAT 
GTTGCCACAG AGGAACCTGT ACAGGAAAGG GAAATTGACT CCACTTCAGA GGAAACAATC 
AGCGATCTCC CTGATCCATC GGAAATCACA AACAATGATT GTGCTGAATC CAATGCACAC 
TGCCAGCTCT CAGAATCCTC CAAGGAGAGC GCAAACACAT CCTCAGAACC TGCTGAATGT 
GTGAATGACC TAGACTTGTC TTGTGCTTTA CCTCAAACAG AAATCGAAGG CAGCAGACCT 
TCTGAGGGCC CCTTAATTGA TGGAGTAACA ATATGTGCTG AAATTCCCAT TGATGACAAA 
GGTGTTGACA TGGCTACCAA TATGAGTAAG ACGACCATCT CTGAGGATTG TCTGTCCTCT 
CCTATGGACA CCACCAGCTG TTCAGTTCTG GAGAGCTCCT CCATGTCTGA CCCACTCAAT 
GCCTCATCCT CAACAGCTGA GCTCACTTCC CCTTTGGATA TTAATAGTGG ATTGCATCAG 
GATTTGGAAC ACACTTCCCC AGTGGATATA GACAATGGTT TGCCTGAGGA ACCGCCGCCG 
TCAGACACAC CTGATTGCCT GAATGGATTA CATCAAGATT CTCCTGATAG TTTGATGGAG 
CACAGTGGAA CTCCTCAGCC ATGTTCTGAG GAACAGTGTG TGGATCAAAT CACAGATGAC 
AGCACTTCAG CAGAGGATGC AGCGGACGGA CACCAAGACT CCTCAGTAGA CATAGCAAAT 
TCCGAAGCGA TCCACAGAGA GACCGAAACA TTTTATTCAG TTGATGCCAG TCAAGACTCT 
GTACTCCAGG AGAAGAAATC TTCTTCAGTG AGCTCAGAAA TCCCCTCTTT GACCCCCTCA 
CCTGAGAGCA TACGTCACAA AGAGTCGCCA AGCACTATGC GAGGGTCCGC CAGCGATGGA 
GACTCTCTGT TTAGTCAGGA TCTGGGCGAG GACAGCGTTT TTAATAATAA GGCTGAGGAC 
AGCATTACCA TCACCAGTGG CGTGTCCATG TCCTATTCGT CCTCCACAGA TGACTCTTCC 
AGCTTGGGAA CCACTTGCGC CACCCCTCAG ACTGGCACAG AGGCTCCTGC ATCAGACCCC 
TTTCAGGAAC AGCCCACATC TACTGGAGCT TCTGGGGAAA CAGAGGAGGA AGAGAAAAAG 
GACCGTCTCA CTGAGGTTCC GGAGCGATCG GCCATTTTAC GGACCACCAT ACGATCACTG 
TCCCCTTTTC GAAGACACAG TTGGGGGCCG GGCAAAAATT TGGCAGGGGA GACAGAGATG 
AGTCAGCGCA GTGCGGCACA AGGTGAAGTG GAGCGCAGAT CTGTTGGTCA TAGGAGAAGC 
ATGAGCTGGT GTCCTTCTGT TGTCCTGCGC TCTGAAAATG ATGAAATGAA TGAAAGAAGT 
TACAGTCTGG AAGGACTTGC ACCAGAGAAG GAGGGAAGGA AAAGTCTGGT TCAGCAGCGT 
GTCGGAGAGT CCAGCCAGGA GCCCAAATGT CCATCAAAGG TGGAAAAAGA AGACAGAGGC 
TCGCTTGTGT CTCTGACTGA AGAAGAGCAG GAGTCAGATC TAGGCGACTG CAGCAGTTTG 
GGCAGTCAGA AGTCCATCCA GTCGGGTCGG AGATGTGTCC CACATTCACA ACCCATCCTC 
ACCAAATCTG TGTCCATGGT GGCCATCAGT CAGAAGGACC TGGACGCAAT GGGACGGCTG 
CGACCCAAGC GACGAATCTC CTTCTCCTTC AGCATCTCGC CGATGCTCCC CAAATCTAAA 
ACTCTCTTTT CTCTTGGCTC TTCATCCAGT GATGAGGAGG AGGCAGTCAG ATTGAGTTCA 
TTCACTGGAA CATCAGGATC ATTAGAGTAC AGTATATCAG AGGAGGATCC TGGGCCATTA 
CGGAGTGACA GTGAAGGCAA AATTGGAGGA ACCAAAGTGA GCAGGACATT CAGTTACCTC 
AAGAGCAAGA TGAGCAAGAA GAACAAGCAG GAGAAGGAGA AAGAGAAGAA TAAGGAGCGT 
GAGCGAGATG CCAAAGACAA GGAGAGAAGA GCCTCAAACG GACATCTCTT CACTACGGTC 
ATGACTGTAC CCACAATCCC CTGCCACCAG TGCAACAAAC TCATCAACAC TAAAGACTCT 
ATCCTCTGCA CCAATTGCAA TGCACAAGTC CACAAAGGCT GCAAAGAGAG TCTTCCTGTC 
TGCGCTAAAG TCAAGATGAA GCAAAAACAG CAGCAGACTG TTACAGATTC TGCCTCGACG 
CCTAGTGTCA CCCTCAGAAG TAAAACTCTT CCAGCAAGGG ATCGTCCGTG GTCCACAATA 
TCCATTCCCG ATGACCAGCC GGCTCCCCTC GCTCCTCCTC GGAAAAGCCC CAGCAGCATC 
ATGTCTTTCA ACAGCAACCC ACTGTCCAAG AGCATGTCCA TTAATAACAT AGCAGGACAA 
ATGGACGACA CTCCTCTTAA GAAGTTTCTG TCCCAGTCAA CGGACTCTCT TCACAAGACC 
AGTAAAGTCA CGGAGTCTAC AGAATCTCTT ACTGATGAGG GTACAGAGAT GATGGACAGC 
CAGCTGATGG GAGAATTTGA AGCTGACGCC AAAGAGCTGG AGGCTGATTC TTATAGCTTC 
ACGGTAGACA AGAAATTTCT TAAGCAGCTT AAAAAGGATG TTATCAAAAG GCAAGACGTC 
ATCTATGAGC TCATCCAGAC AGAAATGCAC CATGTAAGGA CCTTAAAGAT CATGGCTGAT 
GTTTACAGCA AAGGACTACT GAGAGAAGTT CAACTAGAAG CTCAGACGGT GGAGAAGATG 
TTCCCCATGT TGGACGATGT TCTGGATTTG CACACACAGT TCTTTTCACA CCTGCTTGAA 
AGAAAGAAGG AATCAGGGAG CCAAGAAGAG GGCGGCTTTG TCATCCAAAG GATTGGCGAT 
GTGCTGGTTT CTCAGTTCTC AGGTTCCAGT GCTGAGAGAA TGAAGAAGGT CTATGGGAAG 
TTCTGCGGCA GGCACAATGA AGCTGTGAAC TTCTACAAGG AGCTTCACAC CAAAGACAAA 
CGCTTTCAAG CTTTCATCAA GAAAAAGATG AGCAGCCCTG TTGTCCGACG GCTAAGCATT 
CCTGAGTGTA TATTGTTAGT CACACAACGA ATCACAAAAT ATCCCGTGTT ACTACAGAGA 
ATACTGCAGC ACACGAAAGA AAACGAGGAG GACCATGCAG ACGTTAGTCA AGGTCTGAAG 
CTGGTGAAGG AGGTGATTGC TGCCGTGGAC AATAAAGTGA ATGAGCATGA GAAGAAGAAG 
AGGCTAAAGG AAGTGTACAG CCGGACGGAC AACAAGTCCA TCATGAGGAT GAAGAGCGGG 
CAGATGTTTG CCAGAGAGGA TCTGTTACGT GGGCGGAAGT TAATTCACGA CGGGCCGCTG 
CAGCTCAAGA ACTCAGCTGG CAGACTCAAG GATGTCCAGG CTCTGCTGCT GAGGGACGTG 
ATTGTGTTCC TGCAGGAGAA AGATCAGAAG TACATTTTCG CCTCTTTGGA CCAGCGCTCC 
ACCGTCATCT CCCTGCAGAA GCTCATTGTG AGGGAAGTAG CCAATGAGGA GAAAGCTCTG 
TTTCTGATCA CAGCAGGCAT CGAGAAGCCC GAGATGGTAG AAGTGTACAC AAGCTCCAAA 
GAGGAGAGAA ACACATGGAT GCAGCTCATT CAGGAGGCCA TGCAGTCTAT ACTGTCCATT 
TCCTCCAGGG AGAGAGATGA TGATGAAGGT GTGCCAAGTG AAAACGAAGA GGACAGGAGA 
CTTCAGGAAA TCAAGGTCAA GGAGTTCAGA GATCAGCTGC ACAAAAGAGA CGAGCAGATC 
TTGACGTTAC TGGAGGAGAA GGTGAAGTTG TTCAGGGACA TGTGTGACTG TGGTGCACCG 
GACGAGACGG GGCTTCGTAA TCGAATGCTC TTCAGGGCCA CGACGGATGA TGTCACCAGA 
GGAGAGCCAA TCATGAAGGA GGCTCTGAAG GAAGTTGAAA CGCTGCAGGT TCTGGTTAAT 
GACAGTCTTG GCGGTGCGGT TCAGGAAGGT GTGTGCGCGG CTGGACATGT GACTTTGCCA 
CGACGAGCGG AAACATTTGG AGGTTTCGAC AGTCACCAGA TGAACATCTC TAAGAGCAAA 
GATGGAGACA GAGAGGAGCT GGAGGAGTCT GCAGAGCTCC GCAGGACTGA GTCAGACAGT 
GTGTTGAAGA AGACGAGTAA GACCAACCCT TTACTGCTGG TCAGGAGAAA CAATGAGCAG 
GTTCTTCAGA GCGTTGCTCA TCTTCATGAC CTGCTGAACA CATTACAAGC TGTGGTGGTC 
CAGCAGGACA CCTTCATCGA AGACCAGCGT CACGCTCATC CCTCCTCACG CCACCCATCC 
ATCTCCTCCT CTACCTCCTC TTTATCATCC ACCTCATCCC GGCCGAGCTC TCTAATCGAG 
CAGGAGAAAC AGCGCAGCCT GGAGAAGCAG CGGCAGGAGG TGGCCAGCCT CCAAAAACAG 
CAGGCGGCCC ACGCCGAGGA GAGGCGGCGC AGGGAAAAGC AGTGGGAGAC CCGAGAGAAA 
GAGCTAACCC AGAGGGAGAT GCAGATCCAG AGCCAAGAAG AGGAGGCGCA GAGGAGAGCC 
AGACAGCTGG AGGAGGAGAA ACAGGAGCTG CAGAGTAAGA AAGAGGAGTA TCAGAGAGAC 
CTGGCACGAC TGCGGGACAG CCAGAAGAGA CTGGAGAGAG AGAGAGAGCA GGTGCAGAGA 
GAGGCAGATG AGCTTAAACT GACTGAGGAA GTGACAAAGA ACCGGTCTCC GTCAACCACG 
TCTGAAGACT CCACGAAGTT CCAGAGCACC GGTTCATTAG ACCGTGACCC ATCAGAGATG 
GAGCTCTCCT CCTCCCCGTC AGCCAGAGAC TTCCTGTCCC GCATGGACTC CAAACGCAAA 
GGGAAAAACC TCAATCCATT CTCATCCAAC TCAAGCCAGA AGAGCCAGAA CAGCGAGGCT 
CAGAGCCAGA TGCCCAGCCG TCTGCTCCAG CTGGCCAAAC CCAAGGAGAA GAAGGACAAG 
AAGAAGAAGA AGGGGAAAGG CCAGCAGAGC CAGACTGCAG ACTCTCAGAG CACTGTTGTT 
CCAGGCGCTA CCCCAGACGG AGAGATCTTT TTCTGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_021326308.1
CDS	398..8056
Translation	MKLNPQQAPLYGECLLTVQLCEDECVEDEDDVEFYLLFAGSTQRHVSSTLKISHVTLQAVCPAHDRSETVRVTLCQARPGGSVDPVSEECFQFVQDLALDMAQFLVNAAGKTEGALLLDECQIPLKECERLDDTLALALRHLPLPAGWSVLGTDLDTGTCTGSGIAEPGPHDTLLHFAAKRGLRKVALFLLQQPGGREALRLPNKQGRTPAKVAQKRGHSQLHKLLTEVDNLPELETKTSKWCYPEGRVLQHHPKLNTYTLTLEDVPRSSPPSLQCDVEELQRLIQSHQKEDPPTPHKSALLILNRDSSNSTETDDCLPPNSVEPEHQEHNKQEETCKSLLNSLTFEDGLCVHQNGTGDHVQLLDGDVEPDLPAVGDTGNALDKTDCLQRRVDGDSVAFSAPSCGNQREEADIELCIISAKQGVASQPDKGKQEEQEACQKRSETEKDYNTESGNLGLQDMGHAQSQGLLPTETGQPTQREVESEGSTDVCWRGDLPEEREGDMSSNQEIDSYSSALSSHNLEGNSQHDGETEPQLPTQCSEGTDEAKLTLIQVNSEVTLSIRSEDTVISDSVDKPHGLLETETGNLSEHTKGNTESAADLVADDASVKQNSGFSVLEGDLLESLSLRPNMAPGPVHKVQQGLSPVPETSSHVGPALDPFSETTESFQQPNQSTQLLETALECEDVSLNNSTGDINAIAGVSVDISAEVSLCQNEGCPPDGSKEEVAPSERIPDLKELTEEPESVEDIIVSDVSDLDDKSQPFEESSLPIDSECFIRGDFQNDESTVQSSECTGGDINVGTLLGEEEATEKIEQPDSSLKLVELIPEEYTGDATNENKKIEPEEQSILLPTLQGESGFKDVATEEPVQEREIDSTSEETISDLPDPSEITNNDCAESNAHCQLSESSKESANTSSEPAECVNDLDLSCALPQTEIEGSRPSEGPLIDGVTICAEIPIDDKGVDMATNMSKTTISEDCLSSPMDTTSCSVLESSSMSDPLNASSSTAELTSPLDINSGLHQDLEHTSPVDIDNGLPEEPPPSDTPDCLNGLHQDSPDSLMEHSGTPQPCSEEQCVDQITDDSTSAEDAADGHQDSSVDIANSEAIHRETETFYSVDASQDSVLQEKKSSSVSSEIPSLTPSPESIRHKESPSTMRGSASDGDSLFSQDLGEDSVFNNKAEDSITITSGVSMSYSSSTDDSSSLGTTCATPQTGTEAPASDPFQEQPTSTGASGETEEEEKKDRLTEVPERSAILRTTIRSLSPFRRHSWGPGKNLAGETEMSQRSAAQGEVERRSVGHRRSMSWCPSVVLRSENDEMNERSYSLEGLAPEKEGRKSLVQQRVGESSQEPKCPSKVEKEDRGSLVSLTEEEQESDLGDCSSLGSQKSIQSGRRCVPHSQPILTKSVSMVAISQKDLDAMGRLRPKRRISFSFSISPMLPKSKTLFSLGSSSSDEEEAVRLSSFTGTSGSLEYSISEEDPGPLRSDSEGKIGGTKVSRTFSYLKSKMSKKNKQEKEKEKNKERERDAKDKERRASNGHLFTTVMTVPTIPCHQCNKLINTKDSILCTNCNAQVHKGCKESLPVCAKVKMKQKQQQTVTDSASTPSVTLRSKTLPARDRPWSTISIPDDQPAPLAPPRKSPSSIMSFNSNPLSKSMSINNIAGQMDDTPLKKFLSQSTDSLHKTSKVTESTESLTDEGTEMMDSQLMGEFEADAKELEADSYSFTVDKKFLKQLKKDVIKRQDVIYELIQTEMHHVRTLKIMADVYSKGLLREVQLEAQTVEKMFPMLDDVLDLHTQFFSHLLERKKESGSQEEGGFVIQRIGDVLVSQFSGSSAERMKKVYGKFCGRHNEAVNFYKELHTKDKRFQAFIKKKMSSPVVRRLSIPECILLVTQRITKYPVLLQRILQHTKENEEDHADVSQGLKLVKEVIAAVDNKVNEHEKKKRLKEVYSRTDNKSIMRMKSGQMFAREDLLRGRKLIHDGPLQLKNSAGRLKDVQALLLRDVIVFLQEKDQKYIFASLDQRSTVISLQKLIVREVANEEKALFLITAGIEKPEMVEVYTSSKEERNTWMQLIQEAMQSILSISSRERDDDEGVPSENEEDRRLQEIKVKEFRDQLHKRDEQILTLLEEKVKLFRDMCDCGAPDETGLRNRMLFRATTDDVTRGEPIMKEALKEVETLQVLVNDSLGGAVQEGVCAAGHVTLPRRAETFGGFDSHQMNISKSKDGDREELEESAELRRTESDSVLKKTSKTNPLLLVRRNNEQVLQSVAHLHDLLNTLQAVVVQQDTFIEDQRHAHPSSRHPSISSSTSSLSSTSSRPSSLIEQEKQRSLEKQRQEVASLQKQQAAHAEERRRREKQWETREKELTQREMQIQSQEEEAQRRARQLEEEKQELQSKKEEYQRDLARLRDSQKRLEREREQVQREADELKLTEEVTKNRSPSTTSEDSTKFQSTGSLDRDPSEMELSSSPSARDFLSRMDSKRKGKNLNPFSSNSSQKSQNSEAQSQMPSRLLQLAKPKEKKDKKKKKGKGQQSQTADSQSTVVPGATPDGEIFFC

Target ORF information:

RefSeq Version	XM_021470633.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio wu:fi15d04 (wu:fi15d04), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_021470633.1

ORF Insert Sequence:

ATGAAACTGA ATCCACAGCA AGCCCCATTA TATGGAGAGT GTCTGCTGAC GGTCCAGCTC 
TGTGAGGATG AGTGTGTTGA GGATGAGGAT GATGTGGAGT TTTACCTGCT CTTTGCTGGA 
TCCACACAGA GACATGTGTC CAGCACACTG AAGATCAGCC ATGTCACGCT GCAGGCCGTC 
TGTCCAGCTC ATGATCGCAG TGAAACGGTA CGGGTCACAC TATGCCAGGC TCGTCCCGGC 
GGCTCAGTGG ACCCAGTGTC CGAGGAGTGT TTCCAGTTTG TCCAGGACCT GGCTCTGGAC 
ATGGCCCAGT TCCTGGTAAA TGCGGCGGGG AAAACAGAAG GAGCGCTGCT GCTGGACGAG 
TGTCAGATCC CACTGAAAGA GTGCGAGCGG CTGGACGATA CTCTTGCTCT GGCTCTCCGG 
CATCTGCCTT TGCCTGCTGG ATGGAGCGTT TTGGGCACAG ATTTGGACAC GGGCACTTGC 
ACAGGCTCGG GCATCGCAGA GCCTGGACCA CACGATACAC TTTTGCACTT CGCTGCGAAG 
CGCGGACTAC GCAAGGTTGC ACTCTTTCTG CTCCAGCAAC CAGGGGGAAG AGAGGCTCTC 
CGGCTTCCCA ACAAACAGGG TCGCACACCG GCCAAGGTCG CCCAGAAGAG GGGACACTCT 
CAGCTCCACA AACTGCTCAC GGAGGTTGAC AATCTGCCCG AGCTGGAGAC GAAAACATCT 
AAGTGGTGTT ACCCAGAAGG CAGAGTCCTG CAACACCACC CCAAGCTAAA CACTTACACC 
CTTACCCTGG AGGATGTGCC CAGGAGCTCC CCACCAAGCC TTCAGTGTGA TGTGGAGGAG 
CTCCAGCGTC TCATTCAGTC ACATCAGAAG GAAGATCCTC CAACACCGCA TAAGTCAGCA 
CTGCTGATTC TCAATAGAGA CTCCAGTAAT AGCACAGAGA CAGATGACTG CCTTCCGCCC 
AACTCAGTGG AACCTGAGCA TCAGGAGCAC AACAAACAAG AAGAAACTTG CAAATCGCTT 
TTAAACAGCC TTACCTTTGA GGATGGATTG TGTGTTCATC AAAACGGCAC AGGAGATCAT 
GTCCAGTTGC TGGATGGGGA CGTGGAGCCT GACCTGCCAG CTGTAGGAGA TACAGGGAAT 
GCATTAGATA AGACTGACTG TTTGCAGCGG CGGGTAGATG GTGATTCTGT GGCTTTCAGT 
GCCCCTTCCT GTGGAAACCA GCGGGAGGAA GCCGATATAG AGCTCTGCAT AATCAGTGCC 
AAGCAGGGGG TTGCTTCACA GCCTGATAAA GGGAAGCAAG AAGAGCAAGA GGCTTGCCAG 
AAGAGGTCAG AGACTGAAAA GGACTATAAC ACTGAATCTG GCAACCTTGG GCTTCAAGAC 
ATGGGCCACG CACAATCTCA GGGTCTCCTC CCCACGGAAA CTGGTCAGCC TACACAAAGG 
GAGGTGGAGA GTGAGGGGAG TACAGATGTC TGTTGGCGTG GGGACTTACC AGAGGAAAGG 
GAAGGGGACA TGAGTAGCAA CCAGGAAATA GACTCATATT CTTCAGCACT TTCTTCCCAT 
AATCTAGAGG GAAACAGCCA GCACGATGGT GAGACTGAGC CCCAGTTGCC TACACAATGC 
TCAGAGGGAA CAGATGAAGC TAAATTGACT TTAATTCAGG TAAATTCTGA AGTTACTTTG 
TCTATACGAA GCGAGGATAC CGTTATTTCT GACAGTGTTG ACAAGCCACA TGGTCTCTTA 
GAGACGGAGA CAGGAAATCT CAGCGAACAC ACCAAAGGCA ACACTGAGTC AGCTGCTGAT 
TTGGTTGCGG ATGACGCTAG TGTAAAGCAG AACTCTGGAT TTAGCGTTTT AGAGGGGGAT 
CTTTTGGAAT CATTAAGTCT ACGGCCAAAC ATGGCACCGG GACCAGTGCA TAAGGTTCAG 
CAAGGTCTGT CACCAGTTCC TGAGACTTCC TCTCATGTAG GTCCAGCTTT GGACCCCTTT 
TCTGAGACTA CAGAAAGTTT TCAACAGCCG AATCAATCCA CACAGCTTTT AGAAACTGCA 
TTAGAATGTG AGGATGTTTC ATTAAATAAC TCAACCGGTG ACATTAATGC AATAGCAGGG 
GTTTCTGTTG ACATTTCTGC AGAGGTTTCA CTTTGCCAGA ATGAAGGGTG TCCTCCTGAT 
GGAAGCAAAG AAGAGGTAGC CCCCAGTGAG AGGATACCAG ACCTTAAAGA GCTTACGGAA 
GAGCCGGAAT CAGTTGAGGA CATTATTGTC TCAGATGTCT CAGATTTGGA TGACAAATCT 
CAACCTTTTG AAGAGTCTTC TTTGCCTATC GACTCTGAAT GTTTTATAAG AGGTGATTTC 
CAAAACGATG AGTCTACAGT ACAGTCTTCT GAATGTACTG GAGGTGACAT AAATGTAGGA 
ACACTCTTAG GAGAAGAAGA GGCAACTGAA AAAATAGAAC AGCCAGACTC TTCACTTAAA 
CTGGTTGAGC TAATTCCTGA GGAATATACT GGAGATGCCA CAAACGAAAA TAAAAAAATT 
GAGCCAGAGG AACAATCTAT TTTACTGCCA ACTTTGCAAG GGGAATCAGG ATTTAAGGAT 
GTTGCCACAG AGGAACCTGT ACAGGAAAGG GAAATTGACT CCACTTCAGA GGAAACAATC 
AGCGATCTCC CTGATCCATC GGAAATCACA AACAATGATT GTGCTGAATC CAATGCACAC 
TGCCAGCTCT CAGAATCCTC CAAGGAGAGC GCAAACACAT CCTCAGAACC TGCTGAATGT 
GTGAATGACC TAGACTTGTC TTGTGCTTTA CCTCAAACAG AAATCGAAGG CAGCAGACCT 
TCTGAGGGCC CCTTAATTGA TGGAGTAACA ATATGTGCTG AAATTCCCAT TGATGACAAA 
GGTGTTGACA TGGCTACCAA TATGAGTAAG ACGACCATCT CTGAGGATTG TCTGTCCTCT 
CCTATGGACA CCACCAGCTG TTCAGTTCTG GAGAGCTCCT CCATGTCTGA CCCACTCAAT 
GCCTCATCCT CAACAGCTGA GCTCACTTCC CCTTTGGATA TTAATAGTGG ATTGCATCAG 
GATTTGGAAC ACACTTCCCC AGTGGATATA GACAATGGTT TGCCTGAGGA ACCGCCGCCG 
TCAGACACAC CTGATTGCCT GAATGGATTA CATCAAGATT CTCCTGATAG TTTGATGGAG 
CACAGTGGAA CTCCTCAGCC ATGTTCTGAG GAACAGTGTG TGGATCAAAT CACAGATGAC 
AGCACTTCAG CAGAGGATGC AGCGGACGGA CACCAAGACT CCTCAGTAGA CATAGCAAAT 
TCCGAAGCGA TCCACAGAGA GACCGAAACA TTTTATTCAG TTGATGCCAG TCAAGACTCT 
GTACTCCAGG AGAAGAAATC TTCTTCAGTG AGCTCAGAAA TCCCCTCTTT GACCCCCTCA 
CCTGAGAGCA TACGTCACAA AGAGTCGCCA AGCACTATGC GAGGGTCCGC CAGCGATGGA 
GACTCTCTGT TTAGTCAGGA TCTGGGCGAG GACAGCGTTT TTAATAATAA GGCTGAGGAC 
AGCATTACCA TCACCAGTGG CGTGTCCATG TCCTATTCGT CCTCCACAGA TGACTCTTCC 
AGCTTGGGAA CCACTTGCGC CACCCCTCAG ACTGGCACAG AGGCTCCTGC ATCAGACCCC 
TTTCAGGAAC AGCCCACATC TACTGGAGCT TCTGGGGAAA CAGAGGAGGA AGAGAAAAAG 
GACCGTCTCA CTGAGGTTCC GGAGCGATCG GCCATTTTAC GGACCACCAT ACGATCACTG 
TCCCCTTTTC GAAGACACAG TTGGGGGCCG GGCAAAAATT TGGCAGGGGA GACAGAGATG 
AGTCAGCGCA GTGCGGCACA AGGTGAAGTG GAGCGCAGAT CTGTTGGTCA TAGGAGAAGC 
ATGAGCTGGT GTCCTTCTGT TGTCCTGCGC TCTGAAAATG ATGAAATGAA TGAAAGAAGT 
TACAGTCTGG AAGGACTTGC ACCAGAGAAG GAGGGAAGGA AAAGTCTGGT TCAGCAGCGT 
GTCGGAGAGT CCAGCCAGGA GCCCAAATGT CCATCAAAGG TGGAAAAAGA AGACAGAGGC 
TCGCTTGTGT CTCTGACTGA AGAAGAGCAG GAGTCAGATC TAGGCGACTG CAGCAGTTTG 
GGCAGTCAGA AGTCCATCCA GTCGGGTCGG AGATGTGTCC CACATTCACA ACCCATCCTC 
ACCAAATCTG TGTCCATGGT GGCCATCAGT CAGAAGGACC TGGACGCAAT GGGACGGCTG 
CGACCCAAGC GACGAATCTC CTTCTCCTTC AGCATCTCGC CGATGCTCCC CAAATCTAAA 
ACTCTCTTTT CTCTTGGCTC TTCATCCAGT GATGAGGAGG AGGCAGTCAG ATTGAGTTCA 
TTCACTGGAA CATCAGGATC ATTAGAGTAC AGTATATCAG AGGAGGATCC TGGGCCATTA 
CGGAGTGACA GTGAAGGCAA AATTGGAGGA ACCAAAGTGA GCAGGACATT CAGTTACCTC 
AAGAGCAAGA TGAGCAAGAA GAACAAGCAG GAGAAGGAGA AAGAGAAGAA TAAGGAGCGT 
GAGCGAGATG CCAAAGACAA GGAGAGAAGA GCCTCAAACG GACATCTCTT CACTACGGTC 
ATGACTGTAC CCACAATCCC CTGCCACCAG TGCAACAAAC TCATCAACAC TAAAGACTCT 
ATCCTCTGCA CCAATTGCAA TGCACAAGTC CACAAAGGCT GCAAAGAGAG TCTTCCTGTC 
TGCGCTAAAG TCAAGATGAA GCAAAAACAG CAGCAGACTG TTACAGATTC TGCCTCGACG 
CCTAGTGTCA CCCTCAGAAG TAAAACTCTT CCAGCAAGGG ATCGTCCGTG GTCCACAATA 
TCCATTCCCG ATGACCAGCC GGCTCCCCTC GCTCCTCCTC GGAAAAGCCC CAGCAGCATC 
ATGTCTTTCA ACAGCAACCC ACTGTCCAAG AGCATGTCCA TTAATAACAT AGCAGGACAA 
ATGGACGACA CTCCTCTTAA GAAGTTTCTG TCCCAGTCAA CGGACTCTCT TCACAAGACC 
AGTAAAGTCA CGGAGTCTAC AGAATCTCTT ACTGATGAGG GTACAGAGAT GATGGACAGC 
CAGCTGATGG GAGAATTTGA AGCTGACGCC AAAGAGCTGG AGGCTGATTC TTATAGCTTC 
ACGGTAGACA AGAAATTTCT TAAGCAGCTT AAAAAGGATG TTATCAAAAG GCAAGACGTC 
ATCTATGAGC TCATCCAGAC AGAAATGCAC CATGTAAGGA CCTTAAAGAT CATGGCTGAT 
GTTTACAGCA AAGGACTACT GAGAGAAGTT CAACTAGAAG CTCAGACGGT GGAGAAGATG 
TTCCCCATGT TGGACGATGT TCTGGATTTG CACACACAGT TCTTTTCACA CCTGCTTGAA 
AGAAAGAAGG AATCAGGGAG CCAAGAAGAG GGCGGCTTTG TCATCCAAAG GATTGGCGAT 
GTGCTGGTTT CTCAGTTCTC AGGTTCCAGT GCTGAGAGAA TGAAGAAGGT CTATGGGAAG 
TTCTGCGGCA GGCACAATGA AGCTGTGAAC TTCTACAAGG AGCTTCACAC CAAAGACAAA 
CGCTTTCAAG CTTTCATCAA GAAAAAGATG AGCAGCCCTG TTGTCCGACG GCTAAGCATT 
CCTGAGTGTA TATTGTTAGT CACACAACGA ATCACAAAAT ATCCCGTGTT ACTACAGAGA 
ATACTGCAGC ACACGAAAGA AAACGAGGAG GACCATGCAG ACGTTAGTCA AGGTCTGAAG 
CTGGTGAAGG AGGTGATTGC TGCCGTGGAC AATAAAGTGA ATGAGCATGA GAAGAAGAAG 
AGGCTAAAGG AAGTGTACAG CCGGACGGAC AACAAGTCCA TCATGAGGAT GAAGAGCGGG 
CAGATGTTTG CCAGAGAGGA TCTGTTACGT GGGCGGAAGT TAATTCACGA CGGGCCGCTG 
CAGCTCAAGA ACTCAGCTGG CAGACTCAAG GATGTCCAGG CTCTGCTGCT GAGGGACGTG 
ATTGTGTTCC TGCAGGAGAA AGATCAGAAG TACATTTTCG CCTCTTTGGA CCAGCGCTCC 
ACCGTCATCT CCCTGCAGAA GCTCATTGTG AGGGAAGTAG CCAATGAGGA GAAAGCTCTG 
TTTCTGATCA CAGCAGGCAT CGAGAAGCCC GAGATGGTAG AAGTGTACAC AAGCTCCAAA 
GAGGAGAGAA ACACATGGAT GCAGCTCATT CAGGAGGCCA TGCAGTCTAT ACTGTCCATT 
TCCTCCAGGG AGAGAGATGA TGATGAAGGT GTGCCAAGTG AAAACGAAGA GGACAGGAGA 
CTTCAGGAAA TCAAGGTCAA GGAGTTCAGA GATCAGCTGC ACAAAAGAGA CGAGCAGATC 
TTGACGTTAC TGGAGGAGAA GGTGAAGTTG TTCAGGGACA TGTGTGACTG TGGTGCACCG 
GACGAGACGG GGCTTCGTAA TCGAATGCTC TTCAGGGCCA CGACGGATGA TGTCACCAGA 
GGAGAGCCAA TCATGAAGGA GGCTCTGAAG GAAGTTGAAA CGCTGCAGGT TCTGGTTAAT 
GACAGTCTTG GCGGTGCGGT TCAGGAAGGT GTGTGCGCGG CTGGACATGT GACTTTGCCA 
CGACGAGCGG AAACATTTGG AGGTTTCGAC AGTCACCAGA TGAACATCTC TAAGAGCAAA 
GATGGAGACA GAGAGGAGCT GGAGGAGTCT GCAGAGCTCC GCAGGACTGA GTCAGACAGT 
GTGTTGAAGA AGACGAGTAA GACCAACCCT TTACTGCTGG TCAGGAGAAA CAATGAGCAG 
GTTCTTCAGA GCGTTGCTCA TCTTCATGAC CTGCTGAACA CATTACAAGC TGTGGTGGTC 
CAGCAGGACA CCTTCATCGA AGACCAGCGT CACGCTCATC CCTCCTCACG CCACCCATCC 
ATCTCCTCCT CTACCTCCTC TTTATCATCC ACCTCATCCC GGCCGAGCTC TCTAATCGAG 
CAGGAGAAAC AGCGCAGCCT GGAGAAGCAG CGGCAGGAGG TGGCCAGCCT CCAAAAACAG 
CAGGCGGCCC ACGCCGAGGA GAGGCGGCGC AGGGAAAAGC AGTGGGAGAC CCGAGAGAAA 
GAGCTAACCC AGAGGGAGAT GCAGATCCAG AGCCAAGAAG AGGAGGCGCA GAGGAGAGCC 
AGACAGCTGG AGGAGGAGAA ACAGGAGCTG CAGAGTAAGA AAGAGGAGTA TCAGAGAGAC 
CTGGCACGAC TGCGGGACAG CCAGAAGAGA CTGGAGAGAG AGAGAGAGCA GGTGCAGAGA 
GAGGCAGATG AGCTTAAACT GACTGAGGAA GTGACAAAGA ACCGGTCTCC GTCAACCACG 
TCTGAAGACT CCACGAAGTT CCAGAGCACC GGTTCATTAG ACCGTGACCC ATCAGAGATG 
GAGCTCTCCT CCTCCCCGTC AGCCAGAGAC TTCCTGTCCC GCATGGACTC CAAACGCAAA 
GGGAAAAACC TCAATCCATT CTCATCCAAC TCAAGCCAGA AGAGCCAGAA CAGCGAGGCT 
CAGAGCCAGA TGCCCAGCCG TCTGCTCCAG CTGGCCAAAC CCAAGGAGAA GAAGGACAAG 
AAGAAGAAGA AGGGGAAAGG CCAGCAGAGC CAGACTGCAG ACTCTCAGAG CACTGTTGTT 
CCAGGCGCTA CCCCAGACGG AGAGATCTTT TTCTGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

wu:fi15d04 ( XM_021470634.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_021326309.1 Danio rerio wu:fi15d04 (wu:fi15d04), transcript variant X6, mRNA.

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CloneID	ODa141429
Clone ID Related Accession (Same CDS sequence)	XM_021470634.1
Accession Version	XM_021470634.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7644bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	A-kinase anchor protein 13 isoform X6
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007136.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAACTGA ATCCACAGCA AGCCCCATTA TATGGAGAGT GTCTGCTGAC GGTCCAGCTC 
TGTGAGGATG AGTGTGTTGA GGATGAGGAT GATGTGGAGT TTTACCTGCT CTTTGCTGGA 
TCCACACAGA GACATGTGTC CAGCACACTG AAGATCAGCC ATGTCACGCT GCAGGCCGTC 
TGTCCAGCTC ATGATCGCAG TGAAACGGTA CGGGTCACAC TATGCCAGGC TCGTCCCGGC 
GGCTCAGTGG ACCCAGTGTC CGAGGAGTGT TTCCAGTTTG TCCAGGACCT GGCTCTGGAC 
ATGGCCCAGT TCCTGGTAAA TGCGGCGGGG AAAACAGAAG GAGCGCTGCT GCTGGACGAG 
TGTCAGATCC CACTGAAAGA GTGCGAGCGG CTGGACGATA CTCTTGCTCT GGCTCTCCGG 
CATCTGCCTT TGCCTGCTGG ATGGAGCGTT TTGGGCACAG ATTTGGACAC GGGCACTTGC 
ACAGGCTCGG GCATCGCAGA GCCTGGACCA CACGATACAC TTTTGCACTT CGCTGCGAAG 
CGCGGACTAC GCAAGGTTGC ACTCTTTCTG CTCCAGCAAC CAGGGGGAAG AGAGGCTCTC 
CGGCTTCCCA ACAAACAGGG TCGCACACCG GCCAAGGTCG CCCAGAAGAG GGGACACTCT 
CAGCTCCACA AACTGCTCAC GGAGGTTGAC AATCTGCCCG AGCTGGAGAC GAAAACATCT 
AAGTGGTGTT ACCCAGAAGG CAGAGTCCTG CAACACCACC CCAAGCTAAA CACTTACACC 
CTTACCCTGG AGGATGTGCC CAGGAGCTCC CCACCAAGCC TTCAGTGTGA TGTGGAGGAG 
CTCCAGCGTC TCATTCAGTC ACATCAGAAG GAAGATCCTC CAACACCGCA TAAGTCAGCA 
CTGCTGATTC TCAATAGAGA CTCCAGTAAT AGCACAGAGA CAGATGACTG CCTTCCGCCC 
AACTCAGTGG AACCTGAGCA TCAGGAGCAC AACAAACAAG AAGAAACTTG CAAATCGCTT 
TTAAACAGCC TTACCTTTGA GGATGGATTG TGTGTTCATC AAAACGGCAC AGGAGATCAT 
GTCCAGTTGC TGGATGGGGA CGTGGAGCCT GACCTGCCAG CTGTAGGAGA TACAGGGAAT 
GCATTAGATA AGACTGACTG TTTGCAGCGG CGGGTAGATG GTGATTCTGT GGCTTTCAGT 
GCCCCTTCCT GTGGAAACCA GCGGGAGGAA GCCGATATAG AGCTCTGCAT AATCAGTGCC 
AAGCAGGGGG TTGCTTCACA GCCTGATAAA GGGAAGCAAG AAGAGCAAGA GGCTTGCCAG 
AAGAGGTCAG AGACTGAAAA GGACTATAAC ACTGAATCTG GCAACCTTGG GCTTCAAGAC 
ATGGGCCACG CACAATCTCA GGGTCTCCTC CCCACGGAAA CTGGTCAGCC TACACAAAGG 
GAGGTGGAGA GTGAGGGGAG TACAGATGTC TGTTGGCGTG GGGACTTACC AGAGGAAAGG 
GAAGGGGACA TGAGTAGCAA CCAGGAAATA GACTCATATT CTTCAGCACT TTCTTCCCAT 
AATCTAGAGG GAAACAGCCA GCACGATGGT GAGACTGAGC CCCAGTTGCC TACACAATGC 
TCAGAGGGAA CAGATGAAGC TAAATTGACT TTAATTCAGG TAAATTCTGA AGTTACTTTG 
TCTATACGAA GCGAGGATAC CGTTATTTCT GACAGTGTTG ACAAGCCACA TGGTCTCTTA 
GAGACGGAGA CAGGAAATCT CAGCGAACAC ACCAAAGGCA ACACTGAGTC AGCTGCTGAT 
TTGGTTGCGG ATGACGCTAG TGTAAAGCAG AACTCTGGAT TTAGCGTTTT AGAGGGGGAT 
CTTTTGGAAT CATTAAGTCT ACGGCCAAAC ATGGCACCGG GACCAGTGCA TAAGGTTCAG 
CAAGGTCTGT CACCAGTTCC TGAGACTTCC TCTCATGTAG GTCCAGCTTT GGACCCCTTT 
TCTGAGACTA CAGAAAGTTT TCAACAGCCG AATCAATCCA CACAGCTTTT AGAAACTGCA 
TTAGAATGTG AGGATGTTTC ATTAAATAAC TCAACCGGTG ACATTAATGC AATAGCAGGG 
GTTTCTGTTG ACATTTCTGC AGAGGTTTCA CTTTGCCAGA ATGAAGGGTG TCCTCCTGAT 
GGAAGCAAAG AAGAGGTAGC CCCCAGTGAG AGGATACCAG ACCTTAAAGA GCTTACGGAA 
GAGCCGGAAT CAGTTGAGGA CATTATTGTC TCAGATGTCT CAGATTTGGA TGACAAATCT 
CAACCTTTTG AAGAGTCTTC TTTGCCTATC GACTCTGAAT GTTTTATAAG AGGTGATTTC 
CAAAACGATG AGTCTACAGT ACAGTCTTCT GAATGTACTG GAGGTGACAT AAATGTAGGA 
ACACTCTTAG GAGAAGAAGA GGCAACTGAA AAAATAGAAC AGCCAGACTC TTCACTTAAA 
CTGGTTGAGC TAATTCCTGA GGAATATACT GGAGATGCCA CAAACGAAAA TAAAAAAATT 
GAGCCAGAGG AACAATCTAT TTTACTGCCA ACTTTGCAAG GGGAATCAGG ATTTAAGGAT 
GTTGCCACAG AGGAACCTGT ACAGGAAAGG GAAATTGACT CCACTTCAGA GGAAACAATC 
AGCGATCTCC CTGATCCATC GGAAATCACA AACAATGATT GTGCTGAATC CAATGCACAC 
TGCCAGCTCT CAGAATCCTC CAAGGAGAGC GCAAACACAT CCTCAGAACC TGCTGAATGT 
GTGAATGACC TAGACTTGTC TTGTGCTTTA CCTCAAACAG AAATCGAAGG CAGCAGACCT 
TCTGAGGGCC CCTTAATTGA TGGAGTAACA ATATGTGCTG AAATTCCCAT TGATGACAAA 
GGTGTTGACA TGGCTACCAA TATGAGTAAG ACGACCATCT CTGAGGATTG TCTGTCCTCT 
CCTATGGACA CCACCAGCTG TTCAGTTCTG GAGAGCTCCT CCATGTCTGA CCCACTCAAT 
GCCTCATCCT CAACAGCTGA GCTCACTTCC CCTTTGGATA TTAATAGTGG ATTGCATCAG 
GATTTGGAAC ACACTTCCCC AGTGGATATA GACAATGGTT TGCCTGAGGA ACCGCCGCCG 
TCAGACACAC CTGATTGCCT GAATGGATTA CATCAAGATT CTCCTGATAG TTTGATGGAG 
CACAGTGGAA CTCCTCAGCC ATGTTCTGAG GAACAGTGTG TGGATCAAAT CACAGATGAC 
AGCACTTCAG CAGAGGATGC AGCGGACGGA CACCAAGACT CCTCAGTAGA CATAGCAAAT 
TCCGAAGCGA TCCACAGAGA GACCGAAACA TTTTATTCAG TTGATGCCAG TCAAGACTCT 
GTACTCCAGG AGAAGAAATC TTCTTCAGTG AGCTCAGAAA TCCCCTCTTT GACCCCCTCA 
CCTGAGAGCA TACGTCACAA AGAGTCGCCA AGCACTATGC GAGGGTCCGC CAGCGATGGA 
GACTCTCTGT TTAGTCAGGA TCTGGGCGAG GACAGCGTTT TTAATAATAA GGCTGAGGAC 
AGCATTACCA TCACCAGTGG CGTGTCCATG TCCTATTCGT CCTCCACAGA TGACTCTTCC 
AGCTTGGGAA CCACTTGCGC CACCCCTCAG ACTGGCACAG AGGCTCCTGC ATCAGACCCC 
TTTCAGGAAC AGCCCACATC TACTGGAGCT TCTGGGGAAA CAGAGGAGGA AGAGAAAAAG 
GACCGTCTCA CTGAGGTTCC GGAGCGATCG GCCATTTTAC GGACCACCAT ACGATCACTG 
TCCCCTTTTC GAAGACACAG TTGGGGGCCG GGCAAAAATT TGGCAGGGGA GACAGAGATG 
AGTCAGCGCA GTGCGGCACA AGGTGAAGTG GAGCGCAGAT CTGTTGGTCA TAGGAGAAGC 
ATGAGCTGGT GTCCTTCTGT TGTCCTGCGC TCTGAAAATG ATGAAATGAA TGAAAGAAGT 
TACAGTCTGG AAGGACTTGC ACCAGAGAAG GAGGGAAGGA AAAGTCTGGT TCAGCAGCGT 
GTCGGAGAGT CCAGCCAGGA GCCCAAATGT CCATCAAAGG TGGAAAAAGA AGACAGAGGC 
TCGCTTGTGT CTCTGACTGA AGAAGAGCAG GAGTCAGATC TAGGCGACTG CAGCAGTTTG 
GGCAGTCAGA AGTCCATCCA GTCGGGTCGG AGATGTGTCC CACATTCACA ACCCATCCTC 
ACCAAATCTG TGTCCATGGT GGCCATCAGT CAGAAGGACC TGGACGCAAT GGGACGGCTG 
CGACCCAAGC GACGAATCTC CTTCTCCTTC AGCATCTCGC CGATGCTCCC CAAATCTAAA 
ACTCTCTTTT CTCTTGGCTC TTCATCCAGT GATGAGGAGG AGGCAGTCAG ATTGAGTTCA 
TTCACTGGAA CATCAGGATC ATTAGAGTAC AGTATATCAG AGGAGGATCC TGGGCCATTA 
CGGAGTGACA GTGAAGGCAA AATTGGAGGA ACCAAAGTGA GCAGGACATT CAGTTACCTC 
AAGAGCAAGA TGAGCAAGAA GAACAAGCAG GAGAAGGAGA AAGAGAAGAA TAAGGAGCGT 
GAGCGAGATG CCAAAGACAA GGAGAGAAGA GCCTCAAACG GACATCTCTT CACTACGGTC 
ATGACTGTAC CCACAATCCC CTGCCACCAG TGCAACAAAC TCATCAACAC TAAAGACTCT 
ATCCTCTGCA CCAATTGCAA TGCACAAGTC CACAAAGGCT GCAAAGAGAG TCTTCCTGTC 
TGCGCTAAAG TCAAGATGAA GCAGCAAAAA CAGCAGCAGA CTGTTACAGA TTCTGCCTCG 
ACGCCTAGTG TCACCCTCAG AAGTAAAACT CTTCCAGCAA GGGATCGTCC GTGGTCCACA 
ATATCCATTC CCGATGACCA GCCGGCTCCC CTCGCTCCTC CTCGGAAAAG CCCCAGCAGC 
ATCATGTCTT TCAACAGCAA CCCACTGTCC AAGAGCATGT CCATTAATAA CATAGCAGGA 
CAAATGGACG ACACTCCTCT TAAGAAGTTT CTGTCCCAGT CAACGGACTC TCTTCACAAG 
ACCAGTAAAG TCACGGAGTC TACAGAATCT CTTACTGATG AGGGTACAGA GATGATGGAC 
AGCCAGCTGA TGGGAGAATT TGAAGCTGAC GCCAAAGAGC TGGAGGCTGA TTCTTATAGC 
TTCACGGTAG ACAAGAAATT TCTTAAGCAG CTTAAAAAGG ATGTTATCAA AAGGCAAGAC 
GTCATCTATG AGCTCATCCA GACAGAAATG CACCATGTAA GGACCTTAAA GATCATGGCT 
GATGTTTACA GCAAAGGACT ACTGAGAGAA GTTCAACTAG AAGCTCAGAC GGTGGAGAAG 
ATGTTCCCCA TGTTGGACGA TGTTCTGGAT TTGCACACAC AGTTCTTTTC ACACCTGCTT 
GAAAGAAAGA AGGAATCAGG GAGCCAAGAA GAGGGCGGCT TTGTCATCCA AAGGATTGGC 
GATGTGCTGG TTTCTCAGTT CTCAGGTTCC AGTGCTGAGA GAATGAAGAA GGTCTATGGG 
AAGTTCTGCG GCAGGCACAA TGAAGCTGTG AACTTCTACA AGGAGCTTCA CACCAAAGAC 
AAACGCTTTC AAGCTTTCAT CAAGAAAAAG ATGAGCAGCC CTGTTGTCCG ACGGCTAAGC 
ATTCCTGAGT GTATATTGTT AGTCACACAA CGAATCACAA AATATCCCGT GTTACTACAG 
AGAATACTGC AGCACACGAA AGAAAACGAG GAGGACCATG CAGACGTTAG TCAAGGTCTG 
AAGCTGGTGA AGGAGGTGAT TGCTGCCGTG GACAATAAAG TGAATGAGCA TGAGAAGAAG 
AAGAGGCTAA AGGAAGTGTA CAGCCGGACG GACAACAAGT CCATCATGAG GATGAAGAGC 
GGGCAGATGT TTGCCAGAGA GGATCTGTTA CGTGGGCGGA AGTTAATTCA CGACGGGCCG 
CTGCAGCTCA AGAACTCAGC TGGCAGACTC AAGGATGTCC AGGCTCTGCT GCTGAGGGAC 
GTGATTGTGT TCCTGCAGGA GAAAGATCAG AAGTACATTT TCGCCTCTTT GGACCAGCGC 
TCCACCGTCA TCTCCCTGCA GAAGCTCATT GTGAGGGAAG TAGCCAATGA GGAGAAAGCT 
CTGTTTCTGA TCACAGCAGG CATCGAGAAG CCCGAGATGG TAGAAGTGTA CACAAGCTCC 
AAAGAGGAGA GAAACACATG GATGCAGCTC ATTCAGGAGG CCATGCAGTC TATGGAGAGA 
GATGATGATG AAGGTGTGCC AAGTGAAAAC GAAGAGGACA GGAGACTTCA GGAAATCAAG 
GTCAAGGAGT TCAGAGATCA GCTGCACAAA AGAGACGAGC AGATCTTGAC GTTACTGGAG 
GAGAAGGTGA AGTTGTTCAG GGACATGTGT GACTGTGGTG CACCGGACGA GACGGGGCTT 
CGTAATCGAA TGCTCTTCAG GGCCACGACG GATGATGTCA CCAGAGGAGA GCCAATCATG 
AAGGAGGCTC TGAAGGAAGT TGAAACGCTG CAGGTTCTGG TTAATGACAG TCTTGGCGGT 
GCGGTTCAGG AAGGTGTGTG CGCGGCTGGA CATGTGACTT TGCCACGACG AGCGGAAACA 
TTTGGAGGTT TCGACAGTCA CCAGATGAAC ATCTCTAAGA GCAAAGATGG AGACAGAGAG 
GAGCTGGAGG AGTCTGCAGA GCTCCGCAGG ACTGAGTCAG ACAGTGTGTT GAAGAAGACG 
AGTAAGACCA ACCCTTTACT GCTGGTCAGG AGAAACAATG AGCAGGTTCT TCAGAGCGTT 
GCTCATCTTC ATGACCTGCT GAACACATTA CAAGCTGTGG TGGTCCAGCA GGACACCTTC 
ATCGAAGACC AGCGTCACGC TCATCCCTCC TCACGCCACC CATCCATCTC CTCCTCTACC 
TCCTCTTTAT CATCCACCTC ATCCCGGCCG AGCTCTCTAA TCGAGCAGGA GAAACAGCGC 
AGCCTGGAGA AGCAGCGGCA GGAGGTGGCC AGCCTCCAAA AACAGCAGGC GGCCCACGCC 
GAGGAGAGGC GGCGCAGGGA AAAGCAGTGG GAGACCCGAG AGAAAGAGCT AACCCAGAGG 
GAGATGCAGA TCCAGAGCCA AGAAGAGGAG GCGCAGAGGA GAGCCAGACA GCTGGAGGAG 
GAGAAACAGG AGCTGCAGAG TAAGAAAGAG GAGTATCAGA GAGACCTGGC ACGACTGCGG 
GACAGCCAGA AGAGACTGGA GAGAGAGAGA GAGCAGGTGC AGAGAGAGGC AGATGAGCTT 
AAACTGACTG AGGAAGTGAC AAAGAACCGG TCTCCGTCAA CCACGTCTGA AGACTCCACG 
AAGTTCCAGA GCACCGGTTC ATTAGACCGT GACCCATCAG AGATGGAGCT CTCCTCCTCC 
CCGTCAGCCA GAGACTTCCT GTCCCGCATG GACTCCAAAC GCAAAGGGAA AAACCTCAAT 
CCATTCTCAT CCAACTCAAG CCAGAAGAGC CAGAACAGCG AGGCTCAGAG CCAGATGCCC 
AGCCGTCTGC TCCAGCTGGC CAAACCCAAG GAGAAGAAGG ACAAGAAGAA GAAGAAGGGG 
AAAGGCCAGC AGAGCCAGAC TGCAGACTCT CAGAGCACTG TTGTTCCAGG CGCTACCCCA 
GACGGAGAGA TCTTTTTCTG CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_021326309.1
CDS	398..8041
Translation	MKLNPQQAPLYGECLLTVQLCEDECVEDEDDVEFYLLFAGSTQRHVSSTLKISHVTLQAVCPAHDRSETVRVTLCQARPGGSVDPVSEECFQFVQDLALDMAQFLVNAAGKTEGALLLDECQIPLKECERLDDTLALALRHLPLPAGWSVLGTDLDTGTCTGSGIAEPGPHDTLLHFAAKRGLRKVALFLLQQPGGREALRLPNKQGRTPAKVAQKRGHSQLHKLLTEVDNLPELETKTSKWCYPEGRVLQHHPKLNTYTLTLEDVPRSSPPSLQCDVEELQRLIQSHQKEDPPTPHKSALLILNRDSSNSTETDDCLPPNSVEPEHQEHNKQEETCKSLLNSLTFEDGLCVHQNGTGDHVQLLDGDVEPDLPAVGDTGNALDKTDCLQRRVDGDSVAFSAPSCGNQREEADIELCIISAKQGVASQPDKGKQEEQEACQKRSETEKDYNTESGNLGLQDMGHAQSQGLLPTETGQPTQREVESEGSTDVCWRGDLPEEREGDMSSNQEIDSYSSALSSHNLEGNSQHDGETEPQLPTQCSEGTDEAKLTLIQVNSEVTLSIRSEDTVISDSVDKPHGLLETETGNLSEHTKGNTESAADLVADDASVKQNSGFSVLEGDLLESLSLRPNMAPGPVHKVQQGLSPVPETSSHVGPALDPFSETTESFQQPNQSTQLLETALECEDVSLNNSTGDINAIAGVSVDISAEVSLCQNEGCPPDGSKEEVAPSERIPDLKELTEEPESVEDIIVSDVSDLDDKSQPFEESSLPIDSECFIRGDFQNDESTVQSSECTGGDINVGTLLGEEEATEKIEQPDSSLKLVELIPEEYTGDATNENKKIEPEEQSILLPTLQGESGFKDVATEEPVQEREIDSTSEETISDLPDPSEITNNDCAESNAHCQLSESSKESANTSSEPAECVNDLDLSCALPQTEIEGSRPSEGPLIDGVTICAEIPIDDKGVDMATNMSKTTISEDCLSSPMDTTSCSVLESSSMSDPLNASSSTAELTSPLDINSGLHQDLEHTSPVDIDNGLPEEPPPSDTPDCLNGLHQDSPDSLMEHSGTPQPCSEEQCVDQITDDSTSAEDAADGHQDSSVDIANSEAIHRETETFYSVDASQDSVLQEKKSSSVSSEIPSLTPSPESIRHKESPSTMRGSASDGDSLFSQDLGEDSVFNNKAEDSITITSGVSMSYSSSTDDSSSLGTTCATPQTGTEAPASDPFQEQPTSTGASGETEEEEKKDRLTEVPERSAILRTTIRSLSPFRRHSWGPGKNLAGETEMSQRSAAQGEVERRSVGHRRSMSWCPSVVLRSENDEMNERSYSLEGLAPEKEGRKSLVQQRVGESSQEPKCPSKVEKEDRGSLVSLTEEEQESDLGDCSSLGSQKSIQSGRRCVPHSQPILTKSVSMVAISQKDLDAMGRLRPKRRISFSFSISPMLPKSKTLFSLGSSSSDEEEAVRLSSFTGTSGSLEYSISEEDPGPLRSDSEGKIGGTKVSRTFSYLKSKMSKKNKQEKEKEKNKERERDAKDKERRASNGHLFTTVMTVPTIPCHQCNKLINTKDSILCTNCNAQVHKGCKESLPVCAKVKMKQQKQQQTVTDSASTPSVTLRSKTLPARDRPWSTISIPDDQPAPLAPPRKSPSSIMSFNSNPLSKSMSINNIAGQMDDTPLKKFLSQSTDSLHKTSKVTESTESLTDEGTEMMDSQLMGEFEADAKELEADSYSFTVDKKFLKQLKKDVIKRQDVIYELIQTEMHHVRTLKIMADVYSKGLLREVQLEAQTVEKMFPMLDDVLDLHTQFFSHLLERKKESGSQEEGGFVIQRIGDVLVSQFSGSSAERMKKVYGKFCGRHNEAVNFYKELHTKDKRFQAFIKKKMSSPVVRRLSIPECILLVTQRITKYPVLLQRILQHTKENEEDHADVSQGLKLVKEVIAAVDNKVNEHEKKKRLKEVYSRTDNKSIMRMKSGQMFAREDLLRGRKLIHDGPLQLKNSAGRLKDVQALLLRDVIVFLQEKDQKYIFASLDQRSTVISLQKLIVREVANEEKALFLITAGIEKPEMVEVYTSSKEERNTWMQLIQEAMQSMERDDDEGVPSENEEDRRLQEIKVKEFRDQLHKRDEQILTLLEEKVKLFRDMCDCGAPDETGLRNRMLFRATTDDVTRGEPIMKEALKEVETLQVLVNDSLGGAVQEGVCAAGHVTLPRRAETFGGFDSHQMNISKSKDGDREELEESAELRRTESDSVLKKTSKTNPLLLVRRNNEQVLQSVAHLHDLLNTLQAVVVQQDTFIEDQRHAHPSSRHPSISSSTSSLSSTSSRPSSLIEQEKQRSLEKQRQEVASLQKQQAAHAEERRRREKQWETREKELTQREMQIQSQEEEAQRRARQLEEEKQELQSKKEEYQRDLARLRDSQKRLEREREQVQREADELKLTEEVTKNRSPSTTSEDSTKFQSTGSLDRDPSEMELSSSPSARDFLSRMDSKRKGKNLNPFSSNSSQKSQNSEAQSQMPSRLLQLAKPKEKKDKKKKKGKGQQSQTADSQSTVVPGATPDGEIFFC

Target ORF information:

RefSeq Version	XM_021470634.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio wu:fi15d04 (wu:fi15d04), transcript variant X6, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_021470634.1

ORF Insert Sequence:

ATGAAACTGA ATCCACAGCA AGCCCCATTA TATGGAGAGT GTCTGCTGAC GGTCCAGCTC 
TGTGAGGATG AGTGTGTTGA GGATGAGGAT GATGTGGAGT TTTACCTGCT CTTTGCTGGA 
TCCACACAGA GACATGTGTC CAGCACACTG AAGATCAGCC ATGTCACGCT GCAGGCCGTC 
TGTCCAGCTC ATGATCGCAG TGAAACGGTA CGGGTCACAC TATGCCAGGC TCGTCCCGGC 
GGCTCAGTGG ACCCAGTGTC CGAGGAGTGT TTCCAGTTTG TCCAGGACCT GGCTCTGGAC 
ATGGCCCAGT TCCTGGTAAA TGCGGCGGGG AAAACAGAAG GAGCGCTGCT GCTGGACGAG 
TGTCAGATCC CACTGAAAGA GTGCGAGCGG CTGGACGATA CTCTTGCTCT GGCTCTCCGG 
CATCTGCCTT TGCCTGCTGG ATGGAGCGTT TTGGGCACAG ATTTGGACAC GGGCACTTGC 
ACAGGCTCGG GCATCGCAGA GCCTGGACCA CACGATACAC TTTTGCACTT CGCTGCGAAG 
CGCGGACTAC GCAAGGTTGC ACTCTTTCTG CTCCAGCAAC CAGGGGGAAG AGAGGCTCTC 
CGGCTTCCCA ACAAACAGGG TCGCACACCG GCCAAGGTCG CCCAGAAGAG GGGACACTCT 
CAGCTCCACA AACTGCTCAC GGAGGTTGAC AATCTGCCCG AGCTGGAGAC GAAAACATCT 
AAGTGGTGTT ACCCAGAAGG CAGAGTCCTG CAACACCACC CCAAGCTAAA CACTTACACC 
CTTACCCTGG AGGATGTGCC CAGGAGCTCC CCACCAAGCC TTCAGTGTGA TGTGGAGGAG 
CTCCAGCGTC TCATTCAGTC ACATCAGAAG GAAGATCCTC CAACACCGCA TAAGTCAGCA 
CTGCTGATTC TCAATAGAGA CTCCAGTAAT AGCACAGAGA CAGATGACTG CCTTCCGCCC 
AACTCAGTGG AACCTGAGCA TCAGGAGCAC AACAAACAAG AAGAAACTTG CAAATCGCTT 
TTAAACAGCC TTACCTTTGA GGATGGATTG TGTGTTCATC AAAACGGCAC AGGAGATCAT 
GTCCAGTTGC TGGATGGGGA CGTGGAGCCT GACCTGCCAG CTGTAGGAGA TACAGGGAAT 
GCATTAGATA AGACTGACTG TTTGCAGCGG CGGGTAGATG GTGATTCTGT GGCTTTCAGT 
GCCCCTTCCT GTGGAAACCA GCGGGAGGAA GCCGATATAG AGCTCTGCAT AATCAGTGCC 
AAGCAGGGGG TTGCTTCACA GCCTGATAAA GGGAAGCAAG AAGAGCAAGA GGCTTGCCAG 
AAGAGGTCAG AGACTGAAAA GGACTATAAC ACTGAATCTG GCAACCTTGG GCTTCAAGAC 
ATGGGCCACG CACAATCTCA GGGTCTCCTC CCCACGGAAA CTGGTCAGCC TACACAAAGG 
GAGGTGGAGA GTGAGGGGAG TACAGATGTC TGTTGGCGTG GGGACTTACC AGAGGAAAGG 
GAAGGGGACA TGAGTAGCAA CCAGGAAATA GACTCATATT CTTCAGCACT TTCTTCCCAT 
AATCTAGAGG GAAACAGCCA GCACGATGGT GAGACTGAGC CCCAGTTGCC TACACAATGC 
TCAGAGGGAA CAGATGAAGC TAAATTGACT TTAATTCAGG TAAATTCTGA AGTTACTTTG 
TCTATACGAA GCGAGGATAC CGTTATTTCT GACAGTGTTG ACAAGCCACA TGGTCTCTTA 
GAGACGGAGA CAGGAAATCT CAGCGAACAC ACCAAAGGCA ACACTGAGTC AGCTGCTGAT 
TTGGTTGCGG ATGACGCTAG TGTAAAGCAG AACTCTGGAT TTAGCGTTTT AGAGGGGGAT 
CTTTTGGAAT CATTAAGTCT ACGGCCAAAC ATGGCACCGG GACCAGTGCA TAAGGTTCAG 
CAAGGTCTGT CACCAGTTCC TGAGACTTCC TCTCATGTAG GTCCAGCTTT GGACCCCTTT 
TCTGAGACTA CAGAAAGTTT TCAACAGCCG AATCAATCCA CACAGCTTTT AGAAACTGCA 
TTAGAATGTG AGGATGTTTC ATTAAATAAC TCAACCGGTG ACATTAATGC AATAGCAGGG 
GTTTCTGTTG ACATTTCTGC AGAGGTTTCA CTTTGCCAGA ATGAAGGGTG TCCTCCTGAT 
GGAAGCAAAG AAGAGGTAGC CCCCAGTGAG AGGATACCAG ACCTTAAAGA GCTTACGGAA 
GAGCCGGAAT CAGTTGAGGA CATTATTGTC TCAGATGTCT CAGATTTGGA TGACAAATCT 
CAACCTTTTG AAGAGTCTTC TTTGCCTATC GACTCTGAAT GTTTTATAAG AGGTGATTTC 
CAAAACGATG AGTCTACAGT ACAGTCTTCT GAATGTACTG GAGGTGACAT AAATGTAGGA 
ACACTCTTAG GAGAAGAAGA GGCAACTGAA AAAATAGAAC AGCCAGACTC TTCACTTAAA 
CTGGTTGAGC TAATTCCTGA GGAATATACT GGAGATGCCA CAAACGAAAA TAAAAAAATT 
GAGCCAGAGG AACAATCTAT TTTACTGCCA ACTTTGCAAG GGGAATCAGG ATTTAAGGAT 
GTTGCCACAG AGGAACCTGT ACAGGAAAGG GAAATTGACT CCACTTCAGA GGAAACAATC 
AGCGATCTCC CTGATCCATC GGAAATCACA AACAATGATT GTGCTGAATC CAATGCACAC 
TGCCAGCTCT CAGAATCCTC CAAGGAGAGC GCAAACACAT CCTCAGAACC TGCTGAATGT 
GTGAATGACC TAGACTTGTC TTGTGCTTTA CCTCAAACAG AAATCGAAGG CAGCAGACCT 
TCTGAGGGCC CCTTAATTGA TGGAGTAACA ATATGTGCTG AAATTCCCAT TGATGACAAA 
GGTGTTGACA TGGCTACCAA TATGAGTAAG ACGACCATCT CTGAGGATTG TCTGTCCTCT 
CCTATGGACA CCACCAGCTG TTCAGTTCTG GAGAGCTCCT CCATGTCTGA CCCACTCAAT 
GCCTCATCCT CAACAGCTGA GCTCACTTCC CCTTTGGATA TTAATAGTGG ATTGCATCAG 
GATTTGGAAC ACACTTCCCC AGTGGATATA GACAATGGTT TGCCTGAGGA ACCGCCGCCG 
TCAGACACAC CTGATTGCCT GAATGGATTA CATCAAGATT CTCCTGATAG TTTGATGGAG 
CACAGTGGAA CTCCTCAGCC ATGTTCTGAG GAACAGTGTG TGGATCAAAT CACAGATGAC 
AGCACTTCAG CAGAGGATGC AGCGGACGGA CACCAAGACT CCTCAGTAGA CATAGCAAAT 
TCCGAAGCGA TCCACAGAGA GACCGAAACA TTTTATTCAG TTGATGCCAG TCAAGACTCT 
GTACTCCAGG AGAAGAAATC TTCTTCAGTG AGCTCAGAAA TCCCCTCTTT GACCCCCTCA 
CCTGAGAGCA TACGTCACAA AGAGTCGCCA AGCACTATGC GAGGGTCCGC CAGCGATGGA 
GACTCTCTGT TTAGTCAGGA TCTGGGCGAG GACAGCGTTT TTAATAATAA GGCTGAGGAC 
AGCATTACCA TCACCAGTGG CGTGTCCATG TCCTATTCGT CCTCCACAGA TGACTCTTCC 
AGCTTGGGAA CCACTTGCGC CACCCCTCAG ACTGGCACAG AGGCTCCTGC ATCAGACCCC 
TTTCAGGAAC AGCCCACATC TACTGGAGCT TCTGGGGAAA CAGAGGAGGA AGAGAAAAAG 
GACCGTCTCA CTGAGGTTCC GGAGCGATCG GCCATTTTAC GGACCACCAT ACGATCACTG 
TCCCCTTTTC GAAGACACAG TTGGGGGCCG GGCAAAAATT TGGCAGGGGA GACAGAGATG 
AGTCAGCGCA GTGCGGCACA AGGTGAAGTG GAGCGCAGAT CTGTTGGTCA TAGGAGAAGC 
ATGAGCTGGT GTCCTTCTGT TGTCCTGCGC TCTGAAAATG ATGAAATGAA TGAAAGAAGT 
TACAGTCTGG AAGGACTTGC ACCAGAGAAG GAGGGAAGGA AAAGTCTGGT TCAGCAGCGT 
GTCGGAGAGT CCAGCCAGGA GCCCAAATGT CCATCAAAGG TGGAAAAAGA AGACAGAGGC 
TCGCTTGTGT CTCTGACTGA AGAAGAGCAG GAGTCAGATC TAGGCGACTG CAGCAGTTTG 
GGCAGTCAGA AGTCCATCCA GTCGGGTCGG AGATGTGTCC CACATTCACA ACCCATCCTC 
ACCAAATCTG TGTCCATGGT GGCCATCAGT CAGAAGGACC TGGACGCAAT GGGACGGCTG 
CGACCCAAGC GACGAATCTC CTTCTCCTTC AGCATCTCGC CGATGCTCCC CAAATCTAAA 
ACTCTCTTTT CTCTTGGCTC TTCATCCAGT GATGAGGAGG AGGCAGTCAG ATTGAGTTCA 
TTCACTGGAA CATCAGGATC ATTAGAGTAC AGTATATCAG AGGAGGATCC TGGGCCATTA 
CGGAGTGACA GTGAAGGCAA AATTGGAGGA ACCAAAGTGA GCAGGACATT CAGTTACCTC 
AAGAGCAAGA TGAGCAAGAA GAACAAGCAG GAGAAGGAGA AAGAGAAGAA TAAGGAGCGT 
GAGCGAGATG CCAAAGACAA GGAGAGAAGA GCCTCAAACG GACATCTCTT CACTACGGTC 
ATGACTGTAC CCACAATCCC CTGCCACCAG TGCAACAAAC TCATCAACAC TAAAGACTCT 
ATCCTCTGCA CCAATTGCAA TGCACAAGTC CACAAAGGCT GCAAAGAGAG TCTTCCTGTC 
TGCGCTAAAG TCAAGATGAA GCAGCAAAAA CAGCAGCAGA CTGTTACAGA TTCTGCCTCG 
ACGCCTAGTG TCACCCTCAG AAGTAAAACT CTTCCAGCAA GGGATCGTCC GTGGTCCACA 
ATATCCATTC CCGATGACCA GCCGGCTCCC CTCGCTCCTC CTCGGAAAAG CCCCAGCAGC 
ATCATGTCTT TCAACAGCAA CCCACTGTCC AAGAGCATGT CCATTAATAA CATAGCAGGA 
CAAATGGACG ACACTCCTCT TAAGAAGTTT CTGTCCCAGT CAACGGACTC TCTTCACAAG 
ACCAGTAAAG TCACGGAGTC TACAGAATCT CTTACTGATG AGGGTACAGA GATGATGGAC 
AGCCAGCTGA TGGGAGAATT TGAAGCTGAC GCCAAAGAGC TGGAGGCTGA TTCTTATAGC 
TTCACGGTAG ACAAGAAATT TCTTAAGCAG CTTAAAAAGG ATGTTATCAA AAGGCAAGAC 
GTCATCTATG AGCTCATCCA GACAGAAATG CACCATGTAA GGACCTTAAA GATCATGGCT 
GATGTTTACA GCAAAGGACT ACTGAGAGAA GTTCAACTAG AAGCTCAGAC GGTGGAGAAG 
ATGTTCCCCA TGTTGGACGA TGTTCTGGAT TTGCACACAC AGTTCTTTTC ACACCTGCTT 
GAAAGAAAGA AGGAATCAGG GAGCCAAGAA GAGGGCGGCT TTGTCATCCA AAGGATTGGC 
GATGTGCTGG TTTCTCAGTT CTCAGGTTCC AGTGCTGAGA GAATGAAGAA GGTCTATGGG 
AAGTTCTGCG GCAGGCACAA TGAAGCTGTG AACTTCTACA AGGAGCTTCA CACCAAAGAC 
AAACGCTTTC AAGCTTTCAT CAAGAAAAAG ATGAGCAGCC CTGTTGTCCG ACGGCTAAGC 
ATTCCTGAGT GTATATTGTT AGTCACACAA CGAATCACAA AATATCCCGT GTTACTACAG 
AGAATACTGC AGCACACGAA AGAAAACGAG GAGGACCATG CAGACGTTAG TCAAGGTCTG 
AAGCTGGTGA AGGAGGTGAT TGCTGCCGTG GACAATAAAG TGAATGAGCA TGAGAAGAAG 
AAGAGGCTAA AGGAAGTGTA CAGCCGGACG GACAACAAGT CCATCATGAG GATGAAGAGC 
GGGCAGATGT TTGCCAGAGA GGATCTGTTA CGTGGGCGGA AGTTAATTCA CGACGGGCCG 
CTGCAGCTCA AGAACTCAGC TGGCAGACTC AAGGATGTCC AGGCTCTGCT GCTGAGGGAC 
GTGATTGTGT TCCTGCAGGA GAAAGATCAG AAGTACATTT TCGCCTCTTT GGACCAGCGC 
TCCACCGTCA TCTCCCTGCA GAAGCTCATT GTGAGGGAAG TAGCCAATGA GGAGAAAGCT 
CTGTTTCTGA TCACAGCAGG CATCGAGAAG CCCGAGATGG TAGAAGTGTA CACAAGCTCC 
AAAGAGGAGA GAAACACATG GATGCAGCTC ATTCAGGAGG CCATGCAGTC TATGGAGAGA 
GATGATGATG AAGGTGTGCC AAGTGAAAAC GAAGAGGACA GGAGACTTCA GGAAATCAAG 
GTCAAGGAGT TCAGAGATCA GCTGCACAAA AGAGACGAGC AGATCTTGAC GTTACTGGAG 
GAGAAGGTGA AGTTGTTCAG GGACATGTGT GACTGTGGTG CACCGGACGA GACGGGGCTT 
CGTAATCGAA TGCTCTTCAG GGCCACGACG GATGATGTCA CCAGAGGAGA GCCAATCATG 
AAGGAGGCTC TGAAGGAAGT TGAAACGCTG CAGGTTCTGG TTAATGACAG TCTTGGCGGT 
GCGGTTCAGG AAGGTGTGTG CGCGGCTGGA CATGTGACTT TGCCACGACG AGCGGAAACA 
TTTGGAGGTT TCGACAGTCA CCAGATGAAC ATCTCTAAGA GCAAAGATGG AGACAGAGAG 
GAGCTGGAGG AGTCTGCAGA GCTCCGCAGG ACTGAGTCAG ACAGTGTGTT GAAGAAGACG 
AGTAAGACCA ACCCTTTACT GCTGGTCAGG AGAAACAATG AGCAGGTTCT TCAGAGCGTT 
GCTCATCTTC ATGACCTGCT GAACACATTA CAAGCTGTGG TGGTCCAGCA GGACACCTTC 
ATCGAAGACC AGCGTCACGC TCATCCCTCC TCACGCCACC CATCCATCTC CTCCTCTACC 
TCCTCTTTAT CATCCACCTC ATCCCGGCCG AGCTCTCTAA TCGAGCAGGA GAAACAGCGC 
AGCCTGGAGA AGCAGCGGCA GGAGGTGGCC AGCCTCCAAA AACAGCAGGC GGCCCACGCC 
GAGGAGAGGC GGCGCAGGGA AAAGCAGTGG GAGACCCGAG AGAAAGAGCT AACCCAGAGG 
GAGATGCAGA TCCAGAGCCA AGAAGAGGAG GCGCAGAGGA GAGCCAGACA GCTGGAGGAG 
GAGAAACAGG AGCTGCAGAG TAAGAAAGAG GAGTATCAGA GAGACCTGGC ACGACTGCGG 
GACAGCCAGA AGAGACTGGA GAGAGAGAGA GAGCAGGTGC AGAGAGAGGC AGATGAGCTT 
AAACTGACTG AGGAAGTGAC AAAGAACCGG TCTCCGTCAA CCACGTCTGA AGACTCCACG 
AAGTTCCAGA GCACCGGTTC ATTAGACCGT GACCCATCAG AGATGGAGCT CTCCTCCTCC 
CCGTCAGCCA GAGACTTCCT GTCCCGCATG GACTCCAAAC GCAAAGGGAA AAACCTCAAT 
CCATTCTCAT CCAACTCAAG CCAGAAGAGC CAGAACAGCG AGGCTCAGAG CCAGATGCCC 
AGCCGTCTGC TCCAGCTGGC CAAACCCAAG GAGAAGAAGG ACAAGAAGAA GAAGAAGGGG 
AAAGGCCAGC AGAGCCAGAC TGCAGACTCT CAGAGCACTG TTGTTCCAGG CGCTACCCCA 
GACGGAGAGA TCTTTTTCTG CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

wu:fi15d04 ( XM_021470635.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_021326310.1 Danio rerio wu:fi15d04 (wu:fi15d04), transcript variant X7, mRNA.

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CloneID	ODa141430
Clone ID Related Accession (Same CDS sequence)	XM_021470635.1
Accession Version	XM_021470635.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7602bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	A-kinase anchor protein 13 isoform X7
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007136.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAACTGA ATCCACAGCA AGCCCCATTA TATGGAGAGT GTCTGCTGAC GGTCCAGCTC 
TGTGAGGATG AGTGTGTTGA GGATGAGGAT GATGTGGAGT TTTACCTGCT CTTTGCTGGA 
TCCACACAGA GACATGTGTC CAGCACACTG AAGATCAGCC ATGTCACGCT GCAGGCCGTC 
TGTCCAGCTC ATGATCGCAG TGAAACGGTA CGGGTCACAC TATGCCAGGC TCGTCCCGGC 
GGCTCAGTGG ACCCAGTGTC CGAGGAGTGT TTCCAGTTTG TCCAGGACCT GGCTCTGGAC 
ATGGCCCAGT TCCTGGTAAA TGCGGCGGGG AAAACAGAAG GAGCGCTGCT GCTGGACGAG 
TGTCAGATCC CACTGAAAGA GTGCGAGCGG CTGGACGATA CTCTTGCTCT GGCTCTCCGG 
CATCTGCCTT TGCCTGCTGG ATGGAGCGTT TTGGGCACAG ATTTGGACAC GGGCACTTGC 
ACAGGCTCGG GCATCGCAGA GCCTGGACCA CACGATACAC TTTTGCACTT CGCTGCGAAG 
CGCGGACTAC GCAAGGTTGC ACTCTTTCTG CTCCAGCAAC CAGGGGGAAG AGAGGCTCTC 
CGGCTTCCCA ACAAACAGGG TCGCACACCG GCCAAGGTCG CCCAGAAGAG GGGACACTCT 
CAGCTCCACA AACTGCTCAC GGAGGTTGAC AATCTGCCCG AGCTGGAGAC GAAAACATCT 
AAGTGGTGTT ACCCAGAAGG CAGAGTCCTG CAACACCACC CCAAGCTAAA CACTTACACC 
CTTACCCTGG AGGATGTGCC CAGGAGCTCC CCACCAAGCC TTCAGTGTGA TGTGGAGGAG 
CTCCAGCGTC TCATTCAGTC ACATCAGAAG GAAGATCCTC CAACACCGCA TAAGTCAGCA 
CTGCTGATTC TCAATAGAGA CTCCAGTAAT AGCACAGAGA CAGATGACTG CCTTCCGCCC 
AACTCAGTGG AACCTGAGCA TCAGGAGCAC AACAAACAAG AAGAAACTTG CAAATCGCTT 
TTAAACAGCC TTACCTTTGA GGATGGATTG TGTGTTCATC AAAACGGCAC AGGAGATCAT 
GTCCAGTTGC TGGATGGGGA CGTGGAGCCT GACCTGCCAG CTGTAGGAGA TACAGGGAAT 
GCATTAGATA AGACTGACTG TTTGCAGCGG CGGGTAGATG GTGATTCTGT GGCTTTCAGT 
GCCCCTTCCT GTGGAAACCA GCGGGAGGAA GCCGATATAG AGCTCTGCAT AATCAGTGCC 
AAGCAGGGGG TTGCTTCACA GCCTGATAAA GGGAAGCAAG AAGAGCAAGA GGCTTGCCAG 
AAGAGGTCAG AGACTGAAAA GGACTATAAC ACTGAATCTG GCAACCTTGG GCTTCAAGAC 
ATGGGCCACG CACAATCTCA GGGTCTCCTC CCCACGGAAA CTGGTCAGCC TACACAAAGG 
GAGGTGGAGA GTGAGGGGAG TACAGATGTC TGTTGGCGTG GGGACTTACC AGAGGAAAGG 
GAAGGGGACA TGAGTAGCAA CCAGGAAATA GACTCATATT CTTCAGCACT TTCTTCCCAT 
AATCTAGAGG GAAACAGCCA GCACGATGGT GAGACTGAGC CCCAGTTGCC TACACAATGC 
TCAGAGGGAA CAGATGAAGC TAAATTGACT TTAATTCAGG TAAATTCTGA AGTTACTTTG 
TCTATACGAA GCGAGGATAC CGTTATTTCT GACAGTGTTG ACAAGCCACA TGGTCTCTTA 
GAGACGGAGA CAGGAAATCT CAGCGAACAC ACCAAAGGCA ACACTGAGTC AGCTGCTGAT 
TTGGTTGCGG ATGACGCTAG TGTAAAGCAG AACTCTGGAT TTAGCGTTTT AGAGGGGGAT 
CTTTTGGAAT CATTAAGTCT ACGGCCAAAC ATGGCACCGG GACCAGTGCA TAAGGTTCAG 
CAAGGTCTGT CACCAGTTCC TGAGACTTCC TCTCATGTAG GTCCAGCTTT GGACCCCTTT 
TCTGAGACTA CAGAAAGTTT TCAACAGCCG AATCAATCCA CACAGCTTTT AGAAACTGCA 
TTAGAATGTG AGGATGTTTC ATTAAATAAC TCAACCGGTG ACATTAATGC AATAGCAGGG 
GTTTCTGTTG ACATTTCTGC AGAGGTTTCA CTTTGCCAGA ATGAAGGGTG TCCTCCTGAT 
GGAAGCAAAG AAGAGGTAGC CCCCAGTGAG AGGATACCAG ACCTTAAAGA GCTTACGGAA 
GAGCCGGAAT CAGTTGAGGA CATTATTGTC TCAGATGTCT CAGATTTGGA TGACAAATCT 
CAACCTTTTG AAGAGTCTTC TTTGCCTATC GACTCTGAAT GTTTTATAAG AGGTGATTTC 
CAAAACGATG AGTCTACAGT ACAGTCTTCT GAATGTACTG GAGGTGACAT AAATGTAGGA 
ACACTCTTAG GAGAAGAAGA GGCAACTGAA AAAATAGAAC AGCCAGACTC TTCACTTAAA 
CTGGTTGAGC TAATTCCTGA GGAATATACT GGAGATGCCA CAAACGAAAA TAAAAAAATT 
GAGCCAGAGG AACAATCTAT TTTACTGCCA ACTTTGCAAG GGGAATCAGG ATTTAAGGAT 
GTTGCCACAG AGGAACCTGT ACAGGAAAGG GAAATTGACT CCACTTCAGA GGAAACAATC 
AGCGATCTCC CTGATCCATC GGAAATCACA AACAATGATT GTGCTGAATC CAATGCACAC 
TGCCAGCTCT CAGAATCCTC CAAGGAGAGC GCAAACACAT CCTCAGAACC TGCTGAATGT 
GTGAATGACC TAGACTTGTC TTGTGCTTTA CCTCAAACAG AAATCGAAGG CAGCAGACCT 
TCTGAGGGCC CCTTAATTGA TGGAGTAACA ATATGTGCTG AAATTCCCAT TGATGACAAA 
GGTGTTGACA TGGCTACCAA TATGAGTAAG ACGACCATCT CTGAGGATTG TCTGTCCTCT 
CCTATGGACA CCACCAGCTG TTCAGTTCTG GAGAGCTCCT CCATGTCTGA CCCACTCAAT 
GCCTCATCCT CAACAGCTGA GCTCACTTCC CCTTTGGATA TTAATAGTGG ATTGCATCAG 
GATTTGGAAC ACACTTCCCC AGTGGATATA GACAATGGTT TGCCTGAGGA ACCGCCGCCG 
TCAGACACAC CTGATTGCCT GAATGGATTA CATCAAGATT CTCCTGATAG TTTGATGGAG 
CACAGTGGAA CTCCTCAGCC ATGTTCTGAG GAACAGTGTG TGGATCAAAT CACAGATGAC 
AGCACTTCAG CAGAGGATGC AGCGGACGGA CACCAAGACT CCTCAGTAGA CATAGCAAAT 
TCCGAAGCGA TCCACAGAGA GACCGAAACA TTTTATTCAG TTGATGCCAG TCAAGACTCT 
GTACTCCAGG AGAAGAAATC TTCTTCAGTG AGCTCAGAAA TCCCCTCTTT GACCCCCTCA 
CCTGAGAGCA TACGTCACAA AGAGTCGCCA AGCACTATGC GAGGGTCCGC CAGCGATGGA 
GACTCTCTGT TTAGTCAGGA TCTGGGCGAG GACAGCGTTT TTAATAATAA GGCTGAGGAC 
AGCATTACCA TCACCAGTGG CGTGTCCATG TCCTATTCGT CCTCCACAGA TGACTCTTCC 
AGCTTGGGAA CCACTTGCGC CACCCCTCAG ACTGGCACAG AGGCTCCTGC ATCAGACCCC 
TTTCAGGAAC AGCCCACATC TACTGGAGCT TCTGGGGAAA CAGAGGAGGA AGAGAAAAAG 
GACCGTCTCA CTGAGGTTCC GGAGCGATCG GCCATTTTAC GGACCACCAT ACGATCACTG 
TCCCCTTTTC GAAGACACAG TTGGGGGCCG GGCAAAAATT TGGCAGGGGA GACAGAGATG 
AGTCAGCGCA GTGCGGCACA AGGTGAAGTG GAGCGCAGAT CTGTTGGTCA TAGGAGAAGT 
TACAGTCTGG AAGGACTTGC ACCAGAGAAG GAGGGAAGGA AAAGTCTGGT TCAGCAGCGT 
GTCGGAGAGT CCAGCCAGGA GCCCAAATGT CCATCAAAGG TGGAAAAAGA AGACAGAGGC 
TCGCTTGTGT CTCTGACTGA AGAAGAGCAG GAGTCAGATC TAGGCGACTG CAGCAGTTTG 
GGCAGTCAGA AGTCCATCCA GTCGGGTCGG AGATGTGTCC CACATTCACA ACCCATCCTC 
ACCAAATCTG TGTCCATGGT GGCCATCAGT CAGAAGGACC TGGACGCAAT GGGACGGCTG 
CGACCCAAGC GACGAATCTC CTTCTCCTTC AGCATCTCGC CGATGCTCCC CAAATCTAAA 
ACTCTCTTTT CTCTTGGCTC TTCATCCAGT GATGAGGAGG AGGCAGTCAG ATTGAGTTCA 
TTCACTGGAA CATCAGGATC ATTAGAGTAC AGTATATCAG AGGAGGATCC TGGGCCATTA 
CGGAGTGACA GTGAAGGCAA AATTGGAGGA ACCAAAGTGA GCAGGACATT CAGTTACCTC 
AAGAGCAAGA TGAGCAAGAA GAACAAGCAG GAGAAGGAGA AAGAGAAGAA TAAGGAGCGT 
GAGCGAGATG CCAAAGACAA GGAGAGAAGA GCCTCAAACG GACATCTCTT CACTACGGTC 
ATGACTGTAC CCACAATCCC CTGCCACCAG TGCAACAAAC TCATCAACAC TAAAGACTCT 
ATCCTCTGCA CCAATTGCAA TGCACAAGTC CACAAAGGCT GCAAAGAGAG TCTTCCTGTC 
TGCGCTAAAG TCAAGATGAA GCAGCAAAAA CAGCAGCAGA CTGTTACAGA TTCTGCCTCG 
ACGCCTAGTG TCACCCTCAG AAGTAAAACT CTTCCAGCAA GGGATCGTCC GTGGTCCACA 
ATATCCATTC CCGATGACCA GCCGGCTCCC CTCGCTCCTC CTCGGAAAAG CCCCAGCAGC 
ATCATGTCTT TCAACAGCAA CCCACTGTCC AAGAGCATGT CCATTAATAA CATAGCAGGA 
CAAATGGACG ACACTCCTCT TAAGAAGTTT CTGTCCCAGT CAACGGACTC TCTTCACAAG 
ACCAGTAAAG TCACGGAGTC TACAGAATCT CTTACTGATG AGGGTACAGA GATGATGGAC 
AGCCAGCTGA TGGGAGAATT TGAAGCTGAC GCCAAAGAGC TGGAGGCTGA TTCTTATAGC 
TTCACGGTAG ACAAGAAATT TCTTAAGCAG CTTAAAAAGG ATGTTATCAA AAGGCAAGAC 
GTCATCTATG AGCTCATCCA GACAGAAATG CACCATGTAA GGACCTTAAA GATCATGGCT 
GATGTTTACA GCAAAGGACT ACTGAGAGAA GTTCAACTAG AAGCTCAGAC GGTGGAGAAG 
ATGTTCCCCA TGTTGGACGA TGTTCTGGAT TTGCACACAC AGTTCTTTTC ACACCTGCTT 
GAAAGAAAGA AGGAATCAGG GAGCCAAGAA GAGGGCGGCT TTGTCATCCA AAGGATTGGC 
GATGTGCTGG TTTCTCAGTT CTCAGGTTCC AGTGCTGAGA GAATGAAGAA GGTCTATGGG 
AAGTTCTGCG GCAGGCACAA TGAAGCTGTG AACTTCTACA AGGAGCTTCA CACCAAAGAC 
AAACGCTTTC AAGCTTTCAT CAAGAAAAAG ATGAGCAGCC CTGTTGTCCG ACGGCTAAGC 
ATTCCTGAGT GTATATTGTT AGTCACACAA CGAATCACAA AATATCCCGT GTTACTACAG 
AGAATACTGC AGCACACGAA AGAAAACGAG GAGGACCATG CAGACGTTAG TCAAGGTCTG 
AAGCTGGTGA AGGAGGTGAT TGCTGCCGTG GACAATAAAG TGAATGAGCA TGAGAAGAAG 
AAGAGGCTAA AGGAAGTGTA CAGCCGGACG GACAACAAGT CCATCATGAG GATGAAGAGC 
GGGCAGATGT TTGCCAGAGA GGATCTGTTA CGTGGGCGGA AGTTAATTCA CGACGGGCCG 
CTGCAGCTCA AGAACTCAGC TGGCAGACTC AAGGATGTCC AGGCTCTGCT GCTGAGGGAC 
GTGATTGTGT TCCTGCAGGA GAAAGATCAG AAGTACATTT TCGCCTCTTT GGACCAGCGC 
TCCACCGTCA TCTCCCTGCA GAAGCTCATT GTGAGGGAAG TAGCCAATGA GGAGAAAGCT 
CTGTTTCTGA TCACAGCAGG CATCGAGAAG CCCGAGATGG TAGAAGTGTA CACAAGCTCC 
AAAGAGGAGA GAAACACATG GATGCAGCTC ATTCAGGAGG CCATGCAGTC TATACTGTCC 
ATTTCCTCCA GGGAGAGAGA TGATGATGAA GGTGTGCCAA GTGAAAACGA AGAGGACAGG 
AGACTTCAGG AAATCAAGGT CAAGGAGTTC AGAGATCAGC TGCACAAAAG AGACGAGCAG 
ATCTTGACGT TACTGGAGGA GAAGGTGAAG TTGTTCAGGG ACATGTGTGA CTGTGGTGCA 
CCGGACGAGA CGGGGCTTCG TAATCGAATG CTCTTCAGGG CCACGACGGA TGATGTCACC 
AGAGGAGAGC CAATCATGAA GGAGGCTCTG AAGGAAGTTG AAACGCTGCA GGTTCTGGTT 
AATGACAGTC TTGGCGGTGC GGTTCAGGAA GGTGTGTGCG CGGCTGGACA TGTGACTTTG 
CCACGACGAG CGGAAACATT TGGAGGTTTC GACAGTCACC AGATGAACAT CTCTAAGAGC 
AAAGATGGAG ACAGAGAGGA GCTGGAGGAG TCTGCAGAGC TCCGCAGGAC TGAGTCAGAC 
AGTGTGTTGA AGAAGACGAG TAAGACCAAC CCTTTACTGC TGGTCAGGAG AAACAATGAG 
CAGGTTCTTC AGAGCGTTGC TCATCTTCAT GACCTGCTGA ACACATTACA AGCTGTGGTG 
GTCCAGCAGG ACACCTTCAT CGAAGACCAG CGTCACGCTC ATCCCTCCTC ACGCCACCCA 
TCCATCTCCT CCTCTACCTC CTCTTTATCA TCCACCTCAT CCCGGCCGAG CTCTCTAATC 
GAGCAGGAGA AACAGCGCAG CCTGGAGAAG CAGCGGCAGG AGGTGGCCAG CCTCCAAAAA 
CAGCAGGCGG CCCACGCCGA GGAGAGGCGG CGCAGGGAAA AGCAGTGGGA GACCCGAGAG 
AAAGAGCTAA CCCAGAGGGA GATGCAGATC CAGAGCCAAG AAGAGGAGGC GCAGAGGAGA 
GCCAGACAGC TGGAGGAGGA GAAACAGGAG CTGCAGAGTA AGAAAGAGGA GTATCAGAGA 
GACCTGGCAC GACTGCGGGA CAGCCAGAAG AGACTGGAGA GAGAGAGAGA GCAGGTGCAG 
AGAGAGGCAG ATGAGCTTAA ACTGACTGAG GAAGTGACAA AGAACCGGTC TCCGTCAACC 
ACGTCTGAAG ACTCCACGAA GTTCCAGAGC ACCGGTTCAT TAGACCGTGA CCCATCAGAG 
ATGGAGCTCT CCTCCTCCCC GTCAGCCAGA GACTTCCTGT CCCGCATGGA CTCCAAACGC 
AAAGGGAAAA ACCTCAATCC ATTCTCATCC AACTCAAGCC AGAAGAGCCA GAACAGCGAG 
GCTCAGAGCC AGATGCCCAG CCGTCTGCTC CAGCTGGCCA AACCCAAGGA GAAGAAGGAC 
AAGAAGAAGA AGAAGGGGAA AGGCCAGCAG AGCCAGACTG CAGACTCTCA GAGCACTGTT 
GTTCCAGGCG CTACCCCAGA CGGAGAGATC TTTTTCTGCT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_021326310.1
CDS	398..7999
Translation	MKLNPQQAPLYGECLLTVQLCEDECVEDEDDVEFYLLFAGSTQRHVSSTLKISHVTLQAVCPAHDRSETVRVTLCQARPGGSVDPVSEECFQFVQDLALDMAQFLVNAAGKTEGALLLDECQIPLKECERLDDTLALALRHLPLPAGWSVLGTDLDTGTCTGSGIAEPGPHDTLLHFAAKRGLRKVALFLLQQPGGREALRLPNKQGRTPAKVAQKRGHSQLHKLLTEVDNLPELETKTSKWCYPEGRVLQHHPKLNTYTLTLEDVPRSSPPSLQCDVEELQRLIQSHQKEDPPTPHKSALLILNRDSSNSTETDDCLPPNSVEPEHQEHNKQEETCKSLLNSLTFEDGLCVHQNGTGDHVQLLDGDVEPDLPAVGDTGNALDKTDCLQRRVDGDSVAFSAPSCGNQREEADIELCIISAKQGVASQPDKGKQEEQEACQKRSETEKDYNTESGNLGLQDMGHAQSQGLLPTETGQPTQREVESEGSTDVCWRGDLPEEREGDMSSNQEIDSYSSALSSHNLEGNSQHDGETEPQLPTQCSEGTDEAKLTLIQVNSEVTLSIRSEDTVISDSVDKPHGLLETETGNLSEHTKGNTESAADLVADDASVKQNSGFSVLEGDLLESLSLRPNMAPGPVHKVQQGLSPVPETSSHVGPALDPFSETTESFQQPNQSTQLLETALECEDVSLNNSTGDINAIAGVSVDISAEVSLCQNEGCPPDGSKEEVAPSERIPDLKELTEEPESVEDIIVSDVSDLDDKSQPFEESSLPIDSECFIRGDFQNDESTVQSSECTGGDINVGTLLGEEEATEKIEQPDSSLKLVELIPEEYTGDATNENKKIEPEEQSILLPTLQGESGFKDVATEEPVQEREIDSTSEETISDLPDPSEITNNDCAESNAHCQLSESSKESANTSSEPAECVNDLDLSCALPQTEIEGSRPSEGPLIDGVTICAEIPIDDKGVDMATNMSKTTISEDCLSSPMDTTSCSVLESSSMSDPLNASSSTAELTSPLDINSGLHQDLEHTSPVDIDNGLPEEPPPSDTPDCLNGLHQDSPDSLMEHSGTPQPCSEEQCVDQITDDSTSAEDAADGHQDSSVDIANSEAIHRETETFYSVDASQDSVLQEKKSSSVSSEIPSLTPSPESIRHKESPSTMRGSASDGDSLFSQDLGEDSVFNNKAEDSITITSGVSMSYSSSTDDSSSLGTTCATPQTGTEAPASDPFQEQPTSTGASGETEEEEKKDRLTEVPERSAILRTTIRSLSPFRRHSWGPGKNLAGETEMSQRSAAQGEVERRSVGHRRSYSLEGLAPEKEGRKSLVQQRVGESSQEPKCPSKVEKEDRGSLVSLTEEEQESDLGDCSSLGSQKSIQSGRRCVPHSQPILTKSVSMVAISQKDLDAMGRLRPKRRISFSFSISPMLPKSKTLFSLGSSSSDEEEAVRLSSFTGTSGSLEYSISEEDPGPLRSDSEGKIGGTKVSRTFSYLKSKMSKKNKQEKEKEKNKERERDAKDKERRASNGHLFTTVMTVPTIPCHQCNKLINTKDSILCTNCNAQVHKGCKESLPVCAKVKMKQQKQQQTVTDSASTPSVTLRSKTLPARDRPWSTISIPDDQPAPLAPPRKSPSSIMSFNSNPLSKSMSINNIAGQMDDTPLKKFLSQSTDSLHKTSKVTESTESLTDEGTEMMDSQLMGEFEADAKELEADSYSFTVDKKFLKQLKKDVIKRQDVIYELIQTEMHHVRTLKIMADVYSKGLLREVQLEAQTVEKMFPMLDDVLDLHTQFFSHLLERKKESGSQEEGGFVIQRIGDVLVSQFSGSSAERMKKVYGKFCGRHNEAVNFYKELHTKDKRFQAFIKKKMSSPVVRRLSIPECILLVTQRITKYPVLLQRILQHTKENEEDHADVSQGLKLVKEVIAAVDNKVNEHEKKKRLKEVYSRTDNKSIMRMKSGQMFAREDLLRGRKLIHDGPLQLKNSAGRLKDVQALLLRDVIVFLQEKDQKYIFASLDQRSTVISLQKLIVREVANEEKALFLITAGIEKPEMVEVYTSSKEERNTWMQLIQEAMQSILSISSRERDDDEGVPSENEEDRRLQEIKVKEFRDQLHKRDEQILTLLEEKVKLFRDMCDCGAPDETGLRNRMLFRATTDDVTRGEPIMKEALKEVETLQVLVNDSLGGAVQEGVCAAGHVTLPRRAETFGGFDSHQMNISKSKDGDREELEESAELRRTESDSVLKKTSKTNPLLLVRRNNEQVLQSVAHLHDLLNTLQAVVVQQDTFIEDQRHAHPSSRHPSISSSTSSLSSTSSRPSSLIEQEKQRSLEKQRQEVASLQKQQAAHAEERRRREKQWETREKELTQREMQIQSQEEEAQRRARQLEEEKQELQSKKEEYQRDLARLRDSQKRLEREREQVQREADELKLTEEVTKNRSPSTTSEDSTKFQSTGSLDRDPSEMELSSSPSARDFLSRMDSKRKGKNLNPFSSNSSQKSQNSEAQSQMPSRLLQLAKPKEKKDKKKKKGKGQQSQTADSQSTVVPGATPDGEIFFC

Target ORF information:

RefSeq Version	XM_021470635.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio wu:fi15d04 (wu:fi15d04), transcript variant X7, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_021470635.1

ORF Insert Sequence:

ATGAAACTGA ATCCACAGCA AGCCCCATTA TATGGAGAGT GTCTGCTGAC GGTCCAGCTC 
TGTGAGGATG AGTGTGTTGA GGATGAGGAT GATGTGGAGT TTTACCTGCT CTTTGCTGGA 
TCCACACAGA GACATGTGTC CAGCACACTG AAGATCAGCC ATGTCACGCT GCAGGCCGTC 
TGTCCAGCTC ATGATCGCAG TGAAACGGTA CGGGTCACAC TATGCCAGGC TCGTCCCGGC 
GGCTCAGTGG ACCCAGTGTC CGAGGAGTGT TTCCAGTTTG TCCAGGACCT GGCTCTGGAC 
ATGGCCCAGT TCCTGGTAAA TGCGGCGGGG AAAACAGAAG GAGCGCTGCT GCTGGACGAG 
TGTCAGATCC CACTGAAAGA GTGCGAGCGG CTGGACGATA CTCTTGCTCT GGCTCTCCGG 
CATCTGCCTT TGCCTGCTGG ATGGAGCGTT TTGGGCACAG ATTTGGACAC GGGCACTTGC 
ACAGGCTCGG GCATCGCAGA GCCTGGACCA CACGATACAC TTTTGCACTT CGCTGCGAAG 
CGCGGACTAC GCAAGGTTGC ACTCTTTCTG CTCCAGCAAC CAGGGGGAAG AGAGGCTCTC 
CGGCTTCCCA ACAAACAGGG TCGCACACCG GCCAAGGTCG CCCAGAAGAG GGGACACTCT 
CAGCTCCACA AACTGCTCAC GGAGGTTGAC AATCTGCCCG AGCTGGAGAC GAAAACATCT 
AAGTGGTGTT ACCCAGAAGG CAGAGTCCTG CAACACCACC CCAAGCTAAA CACTTACACC 
CTTACCCTGG AGGATGTGCC CAGGAGCTCC CCACCAAGCC TTCAGTGTGA TGTGGAGGAG 
CTCCAGCGTC TCATTCAGTC ACATCAGAAG GAAGATCCTC CAACACCGCA TAAGTCAGCA 
CTGCTGATTC TCAATAGAGA CTCCAGTAAT AGCACAGAGA CAGATGACTG CCTTCCGCCC 
AACTCAGTGG AACCTGAGCA TCAGGAGCAC AACAAACAAG AAGAAACTTG CAAATCGCTT 
TTAAACAGCC TTACCTTTGA GGATGGATTG TGTGTTCATC AAAACGGCAC AGGAGATCAT 
GTCCAGTTGC TGGATGGGGA CGTGGAGCCT GACCTGCCAG CTGTAGGAGA TACAGGGAAT 
GCATTAGATA AGACTGACTG TTTGCAGCGG CGGGTAGATG GTGATTCTGT GGCTTTCAGT 
GCCCCTTCCT GTGGAAACCA GCGGGAGGAA GCCGATATAG AGCTCTGCAT AATCAGTGCC 
AAGCAGGGGG TTGCTTCACA GCCTGATAAA GGGAAGCAAG AAGAGCAAGA GGCTTGCCAG 
AAGAGGTCAG AGACTGAAAA GGACTATAAC ACTGAATCTG GCAACCTTGG GCTTCAAGAC 
ATGGGCCACG CACAATCTCA GGGTCTCCTC CCCACGGAAA CTGGTCAGCC TACACAAAGG 
GAGGTGGAGA GTGAGGGGAG TACAGATGTC TGTTGGCGTG GGGACTTACC AGAGGAAAGG 
GAAGGGGACA TGAGTAGCAA CCAGGAAATA GACTCATATT CTTCAGCACT TTCTTCCCAT 
AATCTAGAGG GAAACAGCCA GCACGATGGT GAGACTGAGC CCCAGTTGCC TACACAATGC 
TCAGAGGGAA CAGATGAAGC TAAATTGACT TTAATTCAGG TAAATTCTGA AGTTACTTTG 
TCTATACGAA GCGAGGATAC CGTTATTTCT GACAGTGTTG ACAAGCCACA TGGTCTCTTA 
GAGACGGAGA CAGGAAATCT CAGCGAACAC ACCAAAGGCA ACACTGAGTC AGCTGCTGAT 
TTGGTTGCGG ATGACGCTAG TGTAAAGCAG AACTCTGGAT TTAGCGTTTT AGAGGGGGAT 
CTTTTGGAAT CATTAAGTCT ACGGCCAAAC ATGGCACCGG GACCAGTGCA TAAGGTTCAG 
CAAGGTCTGT CACCAGTTCC TGAGACTTCC TCTCATGTAG GTCCAGCTTT GGACCCCTTT 
TCTGAGACTA CAGAAAGTTT TCAACAGCCG AATCAATCCA CACAGCTTTT AGAAACTGCA 
TTAGAATGTG AGGATGTTTC ATTAAATAAC TCAACCGGTG ACATTAATGC AATAGCAGGG 
GTTTCTGTTG ACATTTCTGC AGAGGTTTCA CTTTGCCAGA ATGAAGGGTG TCCTCCTGAT 
GGAAGCAAAG AAGAGGTAGC CCCCAGTGAG AGGATACCAG ACCTTAAAGA GCTTACGGAA 
GAGCCGGAAT CAGTTGAGGA CATTATTGTC TCAGATGTCT CAGATTTGGA TGACAAATCT 
CAACCTTTTG AAGAGTCTTC TTTGCCTATC GACTCTGAAT GTTTTATAAG AGGTGATTTC 
CAAAACGATG AGTCTACAGT ACAGTCTTCT GAATGTACTG GAGGTGACAT AAATGTAGGA 
ACACTCTTAG GAGAAGAAGA GGCAACTGAA AAAATAGAAC AGCCAGACTC TTCACTTAAA 
CTGGTTGAGC TAATTCCTGA GGAATATACT GGAGATGCCA CAAACGAAAA TAAAAAAATT 
GAGCCAGAGG AACAATCTAT TTTACTGCCA ACTTTGCAAG GGGAATCAGG ATTTAAGGAT 
GTTGCCACAG AGGAACCTGT ACAGGAAAGG GAAATTGACT CCACTTCAGA GGAAACAATC 
AGCGATCTCC CTGATCCATC GGAAATCACA AACAATGATT GTGCTGAATC CAATGCACAC 
TGCCAGCTCT CAGAATCCTC CAAGGAGAGC GCAAACACAT CCTCAGAACC TGCTGAATGT 
GTGAATGACC TAGACTTGTC TTGTGCTTTA CCTCAAACAG AAATCGAAGG CAGCAGACCT 
TCTGAGGGCC CCTTAATTGA TGGAGTAACA ATATGTGCTG AAATTCCCAT TGATGACAAA 
GGTGTTGACA TGGCTACCAA TATGAGTAAG ACGACCATCT CTGAGGATTG TCTGTCCTCT 
CCTATGGACA CCACCAGCTG TTCAGTTCTG GAGAGCTCCT CCATGTCTGA CCCACTCAAT 
GCCTCATCCT CAACAGCTGA GCTCACTTCC CCTTTGGATA TTAATAGTGG ATTGCATCAG 
GATTTGGAAC ACACTTCCCC AGTGGATATA GACAATGGTT TGCCTGAGGA ACCGCCGCCG 
TCAGACACAC CTGATTGCCT GAATGGATTA CATCAAGATT CTCCTGATAG TTTGATGGAG 
CACAGTGGAA CTCCTCAGCC ATGTTCTGAG GAACAGTGTG TGGATCAAAT CACAGATGAC 
AGCACTTCAG CAGAGGATGC AGCGGACGGA CACCAAGACT CCTCAGTAGA CATAGCAAAT 
TCCGAAGCGA TCCACAGAGA GACCGAAACA TTTTATTCAG TTGATGCCAG TCAAGACTCT 
GTACTCCAGG AGAAGAAATC TTCTTCAGTG AGCTCAGAAA TCCCCTCTTT GACCCCCTCA 
CCTGAGAGCA TACGTCACAA AGAGTCGCCA AGCACTATGC GAGGGTCCGC CAGCGATGGA 
GACTCTCTGT TTAGTCAGGA TCTGGGCGAG GACAGCGTTT TTAATAATAA GGCTGAGGAC 
AGCATTACCA TCACCAGTGG CGTGTCCATG TCCTATTCGT CCTCCACAGA TGACTCTTCC 
AGCTTGGGAA CCACTTGCGC CACCCCTCAG ACTGGCACAG AGGCTCCTGC ATCAGACCCC 
TTTCAGGAAC AGCCCACATC TACTGGAGCT TCTGGGGAAA CAGAGGAGGA AGAGAAAAAG 
GACCGTCTCA CTGAGGTTCC GGAGCGATCG GCCATTTTAC GGACCACCAT ACGATCACTG 
TCCCCTTTTC GAAGACACAG TTGGGGGCCG GGCAAAAATT TGGCAGGGGA GACAGAGATG 
AGTCAGCGCA GTGCGGCACA AGGTGAAGTG GAGCGCAGAT CTGTTGGTCA TAGGAGAAGT 
TACAGTCTGG AAGGACTTGC ACCAGAGAAG GAGGGAAGGA AAAGTCTGGT TCAGCAGCGT 
GTCGGAGAGT CCAGCCAGGA GCCCAAATGT CCATCAAAGG TGGAAAAAGA AGACAGAGGC 
TCGCTTGTGT CTCTGACTGA AGAAGAGCAG GAGTCAGATC TAGGCGACTG CAGCAGTTTG 
GGCAGTCAGA AGTCCATCCA GTCGGGTCGG AGATGTGTCC CACATTCACA ACCCATCCTC 
ACCAAATCTG TGTCCATGGT GGCCATCAGT CAGAAGGACC TGGACGCAAT GGGACGGCTG 
CGACCCAAGC GACGAATCTC CTTCTCCTTC AGCATCTCGC CGATGCTCCC CAAATCTAAA 
ACTCTCTTTT CTCTTGGCTC TTCATCCAGT GATGAGGAGG AGGCAGTCAG ATTGAGTTCA 
TTCACTGGAA CATCAGGATC ATTAGAGTAC AGTATATCAG AGGAGGATCC TGGGCCATTA 
CGGAGTGACA GTGAAGGCAA AATTGGAGGA ACCAAAGTGA GCAGGACATT CAGTTACCTC 
AAGAGCAAGA TGAGCAAGAA GAACAAGCAG GAGAAGGAGA AAGAGAAGAA TAAGGAGCGT 
GAGCGAGATG CCAAAGACAA GGAGAGAAGA GCCTCAAACG GACATCTCTT CACTACGGTC 
ATGACTGTAC CCACAATCCC CTGCCACCAG TGCAACAAAC TCATCAACAC TAAAGACTCT 
ATCCTCTGCA CCAATTGCAA TGCACAAGTC CACAAAGGCT GCAAAGAGAG TCTTCCTGTC 
TGCGCTAAAG TCAAGATGAA GCAGCAAAAA CAGCAGCAGA CTGTTACAGA TTCTGCCTCG 
ACGCCTAGTG TCACCCTCAG AAGTAAAACT CTTCCAGCAA GGGATCGTCC GTGGTCCACA 
ATATCCATTC CCGATGACCA GCCGGCTCCC CTCGCTCCTC CTCGGAAAAG CCCCAGCAGC 
ATCATGTCTT TCAACAGCAA CCCACTGTCC AAGAGCATGT CCATTAATAA CATAGCAGGA 
CAAATGGACG ACACTCCTCT TAAGAAGTTT CTGTCCCAGT CAACGGACTC TCTTCACAAG 
ACCAGTAAAG TCACGGAGTC TACAGAATCT CTTACTGATG AGGGTACAGA GATGATGGAC 
AGCCAGCTGA TGGGAGAATT TGAAGCTGAC GCCAAAGAGC TGGAGGCTGA TTCTTATAGC 
TTCACGGTAG ACAAGAAATT TCTTAAGCAG CTTAAAAAGG ATGTTATCAA AAGGCAAGAC 
GTCATCTATG AGCTCATCCA GACAGAAATG CACCATGTAA GGACCTTAAA GATCATGGCT 
GATGTTTACA GCAAAGGACT ACTGAGAGAA GTTCAACTAG AAGCTCAGAC GGTGGAGAAG 
ATGTTCCCCA TGTTGGACGA TGTTCTGGAT TTGCACACAC AGTTCTTTTC ACACCTGCTT 
GAAAGAAAGA AGGAATCAGG GAGCCAAGAA GAGGGCGGCT TTGTCATCCA AAGGATTGGC 
GATGTGCTGG TTTCTCAGTT CTCAGGTTCC AGTGCTGAGA GAATGAAGAA GGTCTATGGG 
AAGTTCTGCG GCAGGCACAA TGAAGCTGTG AACTTCTACA AGGAGCTTCA CACCAAAGAC 
AAACGCTTTC AAGCTTTCAT CAAGAAAAAG ATGAGCAGCC CTGTTGTCCG ACGGCTAAGC 
ATTCCTGAGT GTATATTGTT AGTCACACAA CGAATCACAA AATATCCCGT GTTACTACAG 
AGAATACTGC AGCACACGAA AGAAAACGAG GAGGACCATG CAGACGTTAG TCAAGGTCTG 
AAGCTGGTGA AGGAGGTGAT TGCTGCCGTG GACAATAAAG TGAATGAGCA TGAGAAGAAG 
AAGAGGCTAA AGGAAGTGTA CAGCCGGACG GACAACAAGT CCATCATGAG GATGAAGAGC 
GGGCAGATGT TTGCCAGAGA GGATCTGTTA CGTGGGCGGA AGTTAATTCA CGACGGGCCG 
CTGCAGCTCA AGAACTCAGC TGGCAGACTC AAGGATGTCC AGGCTCTGCT GCTGAGGGAC 
GTGATTGTGT TCCTGCAGGA GAAAGATCAG AAGTACATTT TCGCCTCTTT GGACCAGCGC 
TCCACCGTCA TCTCCCTGCA GAAGCTCATT GTGAGGGAAG TAGCCAATGA GGAGAAAGCT 
CTGTTTCTGA TCACAGCAGG CATCGAGAAG CCCGAGATGG TAGAAGTGTA CACAAGCTCC 
AAAGAGGAGA GAAACACATG GATGCAGCTC ATTCAGGAGG CCATGCAGTC TATACTGTCC 
ATTTCCTCCA GGGAGAGAGA TGATGATGAA GGTGTGCCAA GTGAAAACGA AGAGGACAGG 
AGACTTCAGG AAATCAAGGT CAAGGAGTTC AGAGATCAGC TGCACAAAAG AGACGAGCAG 
ATCTTGACGT TACTGGAGGA GAAGGTGAAG TTGTTCAGGG ACATGTGTGA CTGTGGTGCA 
CCGGACGAGA CGGGGCTTCG TAATCGAATG CTCTTCAGGG CCACGACGGA TGATGTCACC 
AGAGGAGAGC CAATCATGAA GGAGGCTCTG AAGGAAGTTG AAACGCTGCA GGTTCTGGTT 
AATGACAGTC TTGGCGGTGC GGTTCAGGAA GGTGTGTGCG CGGCTGGACA TGTGACTTTG 
CCACGACGAG CGGAAACATT TGGAGGTTTC GACAGTCACC AGATGAACAT CTCTAAGAGC 
AAAGATGGAG ACAGAGAGGA GCTGGAGGAG TCTGCAGAGC TCCGCAGGAC TGAGTCAGAC 
AGTGTGTTGA AGAAGACGAG TAAGACCAAC CCTTTACTGC TGGTCAGGAG AAACAATGAG 
CAGGTTCTTC AGAGCGTTGC TCATCTTCAT GACCTGCTGA ACACATTACA AGCTGTGGTG 
GTCCAGCAGG ACACCTTCAT CGAAGACCAG CGTCACGCTC ATCCCTCCTC ACGCCACCCA 
TCCATCTCCT CCTCTACCTC CTCTTTATCA TCCACCTCAT CCCGGCCGAG CTCTCTAATC 
GAGCAGGAGA AACAGCGCAG CCTGGAGAAG CAGCGGCAGG AGGTGGCCAG CCTCCAAAAA 
CAGCAGGCGG CCCACGCCGA GGAGAGGCGG CGCAGGGAAA AGCAGTGGGA GACCCGAGAG 
AAAGAGCTAA CCCAGAGGGA GATGCAGATC CAGAGCCAAG AAGAGGAGGC GCAGAGGAGA 
GCCAGACAGC TGGAGGAGGA GAAACAGGAG CTGCAGAGTA AGAAAGAGGA GTATCAGAGA 
GACCTGGCAC GACTGCGGGA CAGCCAGAAG AGACTGGAGA GAGAGAGAGA GCAGGTGCAG 
AGAGAGGCAG ATGAGCTTAA ACTGACTGAG GAAGTGACAA AGAACCGGTC TCCGTCAACC 
ACGTCTGAAG ACTCCACGAA GTTCCAGAGC ACCGGTTCAT TAGACCGTGA CCCATCAGAG 
ATGGAGCTCT CCTCCTCCCC GTCAGCCAGA GACTTCCTGT CCCGCATGGA CTCCAAACGC 
AAAGGGAAAA ACCTCAATCC ATTCTCATCC AACTCAAGCC AGAAGAGCCA GAACAGCGAG 
GCTCAGAGCC AGATGCCCAG CCGTCTGCTC CAGCTGGCCA AACCCAAGGA GAAGAAGGAC 
AAGAAGAAGA AGAAGGGGAA AGGCCAGCAG AGCCAGACTG CAGACTCTCA GAGCACTGTT 
GTTCCAGGCG CTACCCCAGA CGGAGAGATC TTTTTCTGCT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

wu:fi15d04 ( XM_021470636.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_021326311.1 Danio rerio wu:fi15d04 (wu:fi15d04), transcript variant X8, mRNA.

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CloneID	ODa141431
Clone ID Related Accession (Same CDS sequence)	XM_021470636.1
Accession Version	XM_021470636.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7554bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	A-kinase anchor protein 13 isoform X8
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007136.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAACTGA ATCCACAGCA AGCCCCATTA TATGGAGAGT GTCTGCTGAC GGTCCAGCTC 
TGTGAGGATG AGTGTGTTGA GGATGAGGAT GATGTGGAGT TTTACCTGCT CTTTGCTGGA 
TCCACACAGA GACATGTGTC CAGCACACTG AAGATCAGCC ATGTCACGCT GCAGGCCGTC 
TGTCCAGCTC ATGATCGCAG TGAAACGGTA CGGGTCACAC TATGCCAGGC TCGTCCCGGC 
GGCTCAGTGG ACCCAGTGTC CGAGGAGTGT TTCCAGTTTG TCCAGGACCT GGCTCTGGAC 
ATGGCCCAGT TCCTGGTAAA TGCGGCGGGG AAAACAGAAG GAGCGCTGCT GCTGGACGAG 
TGTCAGATCC CACTGAAAGA GTGCGAGCGG CTGGACGATA CTCTTGCTCT GGCTCTCCGG 
CATCTGCCTT TGCCTGCTGG ATGGAGCGTT TTGGGCACAG ATTTGGACAC GGGCACTTGC 
ACAGGCTCGG GCATCGCAGA GCCTGGACCA CACGATACAC TTTTGCACTT CGCTGCGAAG 
CGCGGACTAC GCAAGGTTGC ACTCTTTCTG CTCCAGCAAC CAGGGGGAAG AGAGGCTCTC 
CGGCTTCCCA ACAAACAGGG TCGCACACCG GCCAAGGTCG CCCAGAAGAG GGGACACTCT 
CAGCTCCACA AACTGCTCAC GGAGGTTGAC AATCTGCCCG AGCTGGAGAC GAAAACATCT 
AAGTGGTGTT ACCCAGAAGG CAGAGTCCTG CAACACCACC CCAAGCTAAA CACTTACACC 
CTTACCCTGG AGGATGTGCC CAGGAGCTCC CCACCAAGCC TTCAGTGTGA TGTGGAGGAG 
CTCCAGCGTC TCATTCAGTC ACATCAGAAG GAAGATCCTC CAACACCGCA TAAGTCAGCA 
CTGCTGATTC TCAATAGAGA CTCCAGTAAT AGCACAGAGA CAGATGACTG CCTTCCGCCC 
AACTCAGTGG AACCTGAGCA TCAGGAGCAC AACAAACAAG AAGAAACTTG CAAATCGCTT 
TTAAACAGCC TTACCTTTGA GGATGGATTG TGTGTTCATC AAAACGGCAC AGGAGATCAT 
GTCCAGTTGC TGGATGGGGA CGTGGAGCCT GACCTGCCAG CTGTAGGAGA TACAGGGAAT 
GCATTAGATA AGACTGACTG TTTGCAGCGG CGGGTAGATG GTGATTCTGT GGCTTTCAGT 
GCCCCTTCCT GTGGAAACCA GCGGGAGGAA GCCGATATAG AGCTCTGCAT AATCAGTGCC 
AAGCAGGGGG TTGCTTCACA GCCTGATAAA GGGAAGCAAG AAGAGCAAGA GGCTTGCCAG 
AAGAGGTCAG AGACTGAAAA GGACTATAAC ACTGAATCTG GCAACCTTGG GCTTCAAGAC 
ATGGGCCACG CACAATCTCA GGGTCTCCTC CCCACGGAAA CTGGTCAGCC TACACAAAGG 
GAGGTGGAGA GTGAGGGGAG TACAGATGTC TGTTGGCGTG GGGACTTACC AGAGGAAAGG 
GAAGGGGACA TGAGTAGCAA CCAGGAAATA GACTCATATT CTTCAGCACT TTCTTCCCAT 
AATCTAGAGG GAAACAGCCA GCACGATGGT GAGACTGAGC CCCAGTTGCC TACACAATGC 
TCAGAGGGAA CAGATGAAGC TAAATTGACT TTAATTCAGG TAAATTCTGA AGTTACTTTG 
TCTATACGAA GCGAGGATAC CGTTATTTCT GACAGTGTTG ACAAGCCACA TGGTCTCTTA 
GAGACGGAGA CAGGAAATCT CAGCGAACAC ACCAAAGGCA ACACTGAGTC AGCTGCTGAT 
TTGGTTGCGG ATGACGCTAG TGTAAAGCAG AACTCTGGAT TTAGCGTTTT AGAGGGGGAT 
CTTTTGGAAT CATTAAGTCT ACGGCCAAAC ATGGCACCGG GACCAGTGCA TAAGGTTCAG 
CAAGGTCTGT CACCAGTTCC TGAGACTTCC TCTCATGTAG GTCCAGCTTT GGACCCCTTT 
TCTGAGACTA CAGAAAGTTT TCAACAGCCG AATCAATCCA CACAGCTTTT AGAAACTGCA 
TTAGAATGTG AGGATGTTTC ATTAAATAAC TCAACCGGTG ACATTAATGC AATAGCAGGG 
GTTTCTGTTG ACATTTCTGC AGAGGTTTCA CTTTGCCAGA ATGAAGGGTG TCCTCCTGAT 
GGAAGCAAAG AAGAGGTAGC CCCCAGTGAG AGGATACCAG ACCTTAAAGA GCTTACGGAA 
GAGCCGGAAT CAGTTGAGGA CATTATTGTC TCAGATGTCT CAGATTTGGA TGACAAATCT 
CAACCTTTTG AAGAGTCTTC TTTGCCTATC GACTCTGAAT GTTTTATAAG AGGTGATTTC 
CAAAACGATG AGTCTACAGT ACAGTCTTCT GAATGTACTG GAGGTGACAT AAATGTAGGA 
ACACTCTTAG GAGAAGAAGA GGCAACTGAA AAAATAGAAC AGCCAGACTC TTCACTTAAA 
CTGGTTGAGC TAATTCCTGA GGAATATACT GGAGATGCCA CAAACGAAAA TAAAAAAATT 
GAGCCAGAGG AACAATCTAT TTTACTGCCA ACTTTGCAAG GGGAATCAGG ATTTAAGGAT 
GTTGCCACAG AGGAACCTGT ACAGGAAAGG GAAATTGACT CCACTTCAGA GGAAACAATC 
AGCGATCTCC CTGATCCATC GGAAATCACA AACAATGATT GTGCTGAATC CAATGCACAC 
TGCCAGCTCT CAGAATCCTC CAAGGAGAGC GCAAACACAT CCTCAGAACC TGCTGAATGT 
GTGAATGACC TAGACTTGTC TTGTGCTTTA CCTCAAACAG AAATCGAAGG CAGCAGACCT 
TCTGAGGGCC CCTTAATTGA TGGAGTAACA ATATGTGCTG AAATTCCCAT TGATGACAAA 
GGTGTTGACA TGGCTACCAA TATGAGTAAG ACGACCATCT CTGAGGATTG TCTGTCCTCT 
CCTATGGACA CCACCAGCTG TTCAGTTCTG GAGAGCTCCT CCATGTCTGA CCCACTCAAT 
GCCTCATCCT CAACAGCTGA GCTCACTTCC CCTTTGGATA TTAATAGTGG ATTGCATCAG 
GATTTGGAAC ACACTTCCCC AGTGGATATA GACAATGGTT TGCCTGAGGA ACCGCCGCCG 
TCAGACACAC CTGATTGCCT GAATGGATTA CATCAAGATT CTCCTGATAG TTTGATGGAG 
CACAGTGGAA CTCCTCAGCC ATGTTCTGAG GAACAGTGTG TGGATCAAAT CACAGATGAC 
AGCACTTCAG CAGAGGATGC AGCGGACGGA CACCAAGACT CCTCAGTAGA CATAGCAAAT 
TCCGAAGCGA TCCACAGAGA GACCGAAACA TTTTATTCAG TTGATGCCAG TCAAGACTCT 
GTACTCCAGG AGAAGAAATC TTCTTCAGTG AGCTCAGAAA TCCCCTCTTT GACCCCCTCA 
CCTGAGAGCA TACGTCACAA AGAGTCGCCA AGCACTATGC GAGGGTCCGC CAGCGATGGA 
GACTCTCTGT TTAGTCAGGA TCTGGGCGAG GACAGCGTTT TTAATAATAA GGCTGAGGAC 
AGCATTACCA TCACCAGTGG CGTGTCCATG TCCTATTCGT CCTCCACAGA TGACTCTTCC 
AGCTTGGGAA CCACTTGCGC CACCCCTCAG ACTGGCACAG AGGCTCCTGC ATCAGACCCC 
TTTCAGGAAC AGCCCACATC TACTGGAGCT TCTGGGGAAA CAGAGGAGGA AGAGAAAAAG 
GACCGTCTCA CTGAGGTTCC GGAGCGATCG GCCATTTTAC GGACCACCAT ACGATCACTG 
TCCCCTTTTC GAAGACACAG TTGGGGGCCG GGCAAAAATT TGGCAGGGGA GACAGAGATG 
AGTCAGCGCA GTTACAGTCT GGAAGGACTT GCACCAGAGA AGGAGGGAAG GAAAAGTCTG 
GTTCAGCAGC GTGTCGGAGA GTCCAGCCAG GAGCCCAAAT GTCCATCAAA GGTGGAAAAA 
GAAGACAGAG GCTCGCTTGT GTCTCTGACT GAAGAAGAGC AGGAGTCAGA TCTAGGCGAC 
TGCAGCAGTT TGGGCAGTCA GAAGTCCATC CAGTCGGGTC GGAGATGTGT CCCACATTCA 
CAACCCATCC TCACCAAATC TGTGTCCATG GTGGCCATCA GTCAGAAGGA CCTGGACGCA 
ATGGGACGGC TGCGACCCAA GCGACGAATC TCCTTCTCCT TCAGCATCTC GCCGATGCTC 
CCCAAATCTA AAACTCTCTT TTCTCTTGGC TCTTCATCCA GTGATGAGGA GGAGGCAGTC 
AGATTGAGTT CATTCACTGG AACATCAGGA TCATTAGAGT ACAGTATATC AGAGGAGGAT 
CCTGGGCCAT TACGGAGTGA CAGTGAAGGC AAAATTGGAG GAACCAAAGT GAGCAGGACA 
TTCAGTTACC TCAAGAGCAA GATGAGCAAG AAGAACAAGC AGGAGAAGGA GAAAGAGAAG 
AATAAGGAGC GTGAGCGAGA TGCCAAAGAC AAGGAGAGAA GAGCCTCAAA CGGACATCTC 
TTCACTACGG TCATGACTGT ACCCACAATC CCCTGCCACC AGTGCAACAA ACTCATCAAC 
ACTAAAGACT CTATCCTCTG CACCAATTGC AATGCACAAG TCCACAAAGG CTGCAAAGAG 
AGTCTTCCTG TCTGCGCTAA AGTCAAGATG AAGCAGCAAA AACAGCAGCA GACTGTTACA 
GATTCTGCCT CGACGCCTAG TGTCACCCTC AGAAGTAAAA CTCTTCCAGC AAGGGATCGT 
CCGTGGTCCA CAATATCCAT TCCCGATGAC CAGCCGGCTC CCCTCGCTCC TCCTCGGAAA 
AGCCCCAGCA GCATCATGTC TTTCAACAGC AACCCACTGT CCAAGAGCAT GTCCATTAAT 
AACATAGCAG GACAAATGGA CGACACTCCT CTTAAGAAGT TTCTGTCCCA GTCAACGGAC 
TCTCTTCACA AGACCAGTAA AGTCACGGAG TCTACAGAAT CTCTTACTGA TGAGGGTACA 
GAGATGATGG ACAGCCAGCT GATGGGAGAA TTTGAAGCTG ACGCCAAAGA GCTGGAGGCT 
GATTCTTATA GCTTCACGGT AGACAAGAAA TTTCTTAAGC AGCTTAAAAA GGATGTTATC 
AAAAGGCAAG ACGTCATCTA TGAGCTCATC CAGACAGAAA TGCACCATGT AAGGACCTTA 
AAGATCATGG CTGATGTTTA CAGCAAAGGA CTACTGAGAG AAGTTCAACT AGAAGCTCAG 
ACGGTGGAGA AGATGTTCCC CATGTTGGAC GATGTTCTGG ATTTGCACAC ACAGTTCTTT 
TCACACCTGC TTGAAAGAAA GAAGGAATCA GGGAGCCAAG AAGAGGGCGG CTTTGTCATC 
CAAAGGATTG GCGATGTGCT GGTTTCTCAG TTCTCAGGTT CCAGTGCTGA GAGAATGAAG 
AAGGTCTATG GGAAGTTCTG CGGCAGGCAC AATGAAGCTG TGAACTTCTA CAAGGAGCTT 
CACACCAAAG ACAAACGCTT TCAAGCTTTC ATCAAGAAAA AGATGAGCAG CCCTGTTGTC 
CGACGGCTAA GCATTCCTGA GTGTATATTG TTAGTCACAC AACGAATCAC AAAATATCCC 
GTGTTACTAC AGAGAATACT GCAGCACACG AAAGAAAACG AGGAGGACCA TGCAGACGTT 
AGTCAAGGTC TGAAGCTGGT GAAGGAGGTG ATTGCTGCCG TGGACAATAA AGTGAATGAG 
CATGAGAAGA AGAAGAGGCT AAAGGAAGTG TACAGCCGGA CGGACAACAA GTCCATCATG 
AGGATGAAGA GCGGGCAGAT GTTTGCCAGA GAGGATCTGT TACGTGGGCG GAAGTTAATT 
CACGACGGGC CGCTGCAGCT CAAGAACTCA GCTGGCAGAC TCAAGGATGT CCAGGCTCTG 
CTGCTGAGGG ACGTGATTGT GTTCCTGCAG GAGAAAGATC AGAAGTACAT TTTCGCCTCT 
TTGGACCAGC GCTCCACCGT CATCTCCCTG CAGAAGCTCA TTGTGAGGGA AGTAGCCAAT 
GAGGAGAAAG CTCTGTTTCT GATCACAGCA GGCATCGAGA AGCCCGAGAT GGTAGAAGTG 
TACACAAGCT CCAAAGAGGA GAGAAACACA TGGATGCAGC TCATTCAGGA GGCCATGCAG 
TCTATACTGT CCATTTCCTC CAGGGAGAGA GATGATGATG AAGGTGTGCC AAGTGAAAAC 
GAAGAGGACA GGAGACTTCA GGAAATCAAG GTCAAGGAGT TCAGAGATCA GCTGCACAAA 
AGAGACGAGC AGATCTTGAC GTTACTGGAG GAGAAGGTGA AGTTGTTCAG GGACATGTGT 
GACTGTGGTG CACCGGACGA GACGGGGCTT CGTAATCGAA TGCTCTTCAG GGCCACGACG 
GATGATGTCA CCAGAGGAGA GCCAATCATG AAGGAGGCTC TGAAGGAAGT TGAAACGCTG 
CAGGTTCTGG TTAATGACAG TCTTGGCGGT GCGGTTCAGG AAGGTGTGTG CGCGGCTGGA 
CATGTGACTT TGCCACGACG AGCGGAAACA TTTGGAGGTT TCGACAGTCA CCAGATGAAC 
ATCTCTAAGA GCAAAGATGG AGACAGAGAG GAGCTGGAGG AGTCTGCAGA GCTCCGCAGG 
ACTGAGTCAG ACAGTGTGTT GAAGAAGACG AGTAAGACCA ACCCTTTACT GCTGGTCAGG 
AGAAACAATG AGCAGGTTCT TCAGAGCGTT GCTCATCTTC ATGACCTGCT GAACACATTA 
CAAGCTGTGG TGGTCCAGCA GGACACCTTC ATCGAAGACC AGCGTCACGC TCATCCCTCC 
TCACGCCACC CATCCATCTC CTCCTCTACC TCCTCTTTAT CATCCACCTC ATCCCGGCCG 
AGCTCTCTAA TCGAGCAGGA GAAACAGCGC AGCCTGGAGA AGCAGCGGCA GGAGGTGGCC 
AGCCTCCAAA AACAGCAGGC GGCCCACGCC GAGGAGAGGC GGCGCAGGGA AAAGCAGTGG 
GAGACCCGAG AGAAAGAGCT AACCCAGAGG GAGATGCAGA TCCAGAGCCA AGAAGAGGAG 
GCGCAGAGGA GAGCCAGACA GCTGGAGGAG GAGAAACAGG AGCTGCAGAG TAAGAAAGAG 
GAGTATCAGA GAGACCTGGC ACGACTGCGG GACAGCCAGA AGAGACTGGA GAGAGAGAGA 
GAGCAGGTGC AGAGAGAGGC AGATGAGCTT AAACTGACTG AGGAAGTGAC AAAGAACCGG 
TCTCCGTCAA CCACGTCTGA AGACTCCACG AAGTTCCAGA GCACCGGTTC ATTAGACCGT 
GACCCATCAG AGATGGAGCT CTCCTCCTCC CCGTCAGCCA GAGACTTCCT GTCCCGCATG 
GACTCCAAAC GCAAAGGGAA AAACCTCAAT CCATTCTCAT CCAACTCAAG CCAGAAGAGC 
CAGAACAGCG AGGCTCAGAG CCAGATGCCC AGCCGTCTGC TCCAGCTGGC CAAACCCAAG 
GAGAAGAAGG ACAAGAAGAA GAAGAAGGGG AAAGGCCAGC AGAGCCAGAC TGCAGACTCT 
CAGAGCACTG TTGTTCCAGG CGCTACCCCA GACGGAGAGA TCTTTTTCTG CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_021326311.1
CDS	398..7951
Translation	MKLNPQQAPLYGECLLTVQLCEDECVEDEDDVEFYLLFAGSTQRHVSSTLKISHVTLQAVCPAHDRSETVRVTLCQARPGGSVDPVSEECFQFVQDLALDMAQFLVNAAGKTEGALLLDECQIPLKECERLDDTLALALRHLPLPAGWSVLGTDLDTGTCTGSGIAEPGPHDTLLHFAAKRGLRKVALFLLQQPGGREALRLPNKQGRTPAKVAQKRGHSQLHKLLTEVDNLPELETKTSKWCYPEGRVLQHHPKLNTYTLTLEDVPRSSPPSLQCDVEELQRLIQSHQKEDPPTPHKSALLILNRDSSNSTETDDCLPPNSVEPEHQEHNKQEETCKSLLNSLTFEDGLCVHQNGTGDHVQLLDGDVEPDLPAVGDTGNALDKTDCLQRRVDGDSVAFSAPSCGNQREEADIELCIISAKQGVASQPDKGKQEEQEACQKRSETEKDYNTESGNLGLQDMGHAQSQGLLPTETGQPTQREVESEGSTDVCWRGDLPEEREGDMSSNQEIDSYSSALSSHNLEGNSQHDGETEPQLPTQCSEGTDEAKLTLIQVNSEVTLSIRSEDTVISDSVDKPHGLLETETGNLSEHTKGNTESAADLVADDASVKQNSGFSVLEGDLLESLSLRPNMAPGPVHKVQQGLSPVPETSSHVGPALDPFSETTESFQQPNQSTQLLETALECEDVSLNNSTGDINAIAGVSVDISAEVSLCQNEGCPPDGSKEEVAPSERIPDLKELTEEPESVEDIIVSDVSDLDDKSQPFEESSLPIDSECFIRGDFQNDESTVQSSECTGGDINVGTLLGEEEATEKIEQPDSSLKLVELIPEEYTGDATNENKKIEPEEQSILLPTLQGESGFKDVATEEPVQEREIDSTSEETISDLPDPSEITNNDCAESNAHCQLSESSKESANTSSEPAECVNDLDLSCALPQTEIEGSRPSEGPLIDGVTICAEIPIDDKGVDMATNMSKTTISEDCLSSPMDTTSCSVLESSSMSDPLNASSSTAELTSPLDINSGLHQDLEHTSPVDIDNGLPEEPPPSDTPDCLNGLHQDSPDSLMEHSGTPQPCSEEQCVDQITDDSTSAEDAADGHQDSSVDIANSEAIHRETETFYSVDASQDSVLQEKKSSSVSSEIPSLTPSPESIRHKESPSTMRGSASDGDSLFSQDLGEDSVFNNKAEDSITITSGVSMSYSSSTDDSSSLGTTCATPQTGTEAPASDPFQEQPTSTGASGETEEEEKKDRLTEVPERSAILRTTIRSLSPFRRHSWGPGKNLAGETEMSQRSYSLEGLAPEKEGRKSLVQQRVGESSQEPKCPSKVEKEDRGSLVSLTEEEQESDLGDCSSLGSQKSIQSGRRCVPHSQPILTKSVSMVAISQKDLDAMGRLRPKRRISFSFSISPMLPKSKTLFSLGSSSSDEEEAVRLSSFTGTSGSLEYSISEEDPGPLRSDSEGKIGGTKVSRTFSYLKSKMSKKNKQEKEKEKNKERERDAKDKERRASNGHLFTTVMTVPTIPCHQCNKLINTKDSILCTNCNAQVHKGCKESLPVCAKVKMKQQKQQQTVTDSASTPSVTLRSKTLPARDRPWSTISIPDDQPAPLAPPRKSPSSIMSFNSNPLSKSMSINNIAGQMDDTPLKKFLSQSTDSLHKTSKVTESTESLTDEGTEMMDSQLMGEFEADAKELEADSYSFTVDKKFLKQLKKDVIKRQDVIYELIQTEMHHVRTLKIMADVYSKGLLREVQLEAQTVEKMFPMLDDVLDLHTQFFSHLLERKKESGSQEEGGFVIQRIGDVLVSQFSGSSAERMKKVYGKFCGRHNEAVNFYKELHTKDKRFQAFIKKKMSSPVVRRLSIPECILLVTQRITKYPVLLQRILQHTKENEEDHADVSQGLKLVKEVIAAVDNKVNEHEKKKRLKEVYSRTDNKSIMRMKSGQMFAREDLLRGRKLIHDGPLQLKNSAGRLKDVQALLLRDVIVFLQEKDQKYIFASLDQRSTVISLQKLIVREVANEEKALFLITAGIEKPEMVEVYTSSKEERNTWMQLIQEAMQSILSISSRERDDDEGVPSENEEDRRLQEIKVKEFRDQLHKRDEQILTLLEEKVKLFRDMCDCGAPDETGLRNRMLFRATTDDVTRGEPIMKEALKEVETLQVLVNDSLGGAVQEGVCAAGHVTLPRRAETFGGFDSHQMNISKSKDGDREELEESAELRRTESDSVLKKTSKTNPLLLVRRNNEQVLQSVAHLHDLLNTLQAVVVQQDTFIEDQRHAHPSSRHPSISSSTSSLSSTSSRPSSLIEQEKQRSLEKQRQEVASLQKQQAAHAEERRRREKQWETREKELTQREMQIQSQEEEAQRRARQLEEEKQELQSKKEEYQRDLARLRDSQKRLEREREQVQREADELKLTEEVTKNRSPSTTSEDSTKFQSTGSLDRDPSEMELSSSPSARDFLSRMDSKRKGKNLNPFSSNSSQKSQNSEAQSQMPSRLLQLAKPKEKKDKKKKKGKGQQSQTADSQSTVVPGATPDGEIFFC

Target ORF information:

RefSeq Version	XM_021470636.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio wu:fi15d04 (wu:fi15d04), transcript variant X8, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_021470636.1

ORF Insert Sequence:

ATGAAACTGA ATCCACAGCA AGCCCCATTA TATGGAGAGT GTCTGCTGAC GGTCCAGCTC 
TGTGAGGATG AGTGTGTTGA GGATGAGGAT GATGTGGAGT TTTACCTGCT CTTTGCTGGA 
TCCACACAGA GACATGTGTC CAGCACACTG AAGATCAGCC ATGTCACGCT GCAGGCCGTC 
TGTCCAGCTC ATGATCGCAG TGAAACGGTA CGGGTCACAC TATGCCAGGC TCGTCCCGGC 
GGCTCAGTGG ACCCAGTGTC CGAGGAGTGT TTCCAGTTTG TCCAGGACCT GGCTCTGGAC 
ATGGCCCAGT TCCTGGTAAA TGCGGCGGGG AAAACAGAAG GAGCGCTGCT GCTGGACGAG 
TGTCAGATCC CACTGAAAGA GTGCGAGCGG CTGGACGATA CTCTTGCTCT GGCTCTCCGG 
CATCTGCCTT TGCCTGCTGG ATGGAGCGTT TTGGGCACAG ATTTGGACAC GGGCACTTGC 
ACAGGCTCGG GCATCGCAGA GCCTGGACCA CACGATACAC TTTTGCACTT CGCTGCGAAG 
CGCGGACTAC GCAAGGTTGC ACTCTTTCTG CTCCAGCAAC CAGGGGGAAG AGAGGCTCTC 
CGGCTTCCCA ACAAACAGGG TCGCACACCG GCCAAGGTCG CCCAGAAGAG GGGACACTCT 
CAGCTCCACA AACTGCTCAC GGAGGTTGAC AATCTGCCCG AGCTGGAGAC GAAAACATCT 
AAGTGGTGTT ACCCAGAAGG CAGAGTCCTG CAACACCACC CCAAGCTAAA CACTTACACC 
CTTACCCTGG AGGATGTGCC CAGGAGCTCC CCACCAAGCC TTCAGTGTGA TGTGGAGGAG 
CTCCAGCGTC TCATTCAGTC ACATCAGAAG GAAGATCCTC CAACACCGCA TAAGTCAGCA 
CTGCTGATTC TCAATAGAGA CTCCAGTAAT AGCACAGAGA CAGATGACTG CCTTCCGCCC 
AACTCAGTGG AACCTGAGCA TCAGGAGCAC AACAAACAAG AAGAAACTTG CAAATCGCTT 
TTAAACAGCC TTACCTTTGA GGATGGATTG TGTGTTCATC AAAACGGCAC AGGAGATCAT 
GTCCAGTTGC TGGATGGGGA CGTGGAGCCT GACCTGCCAG CTGTAGGAGA TACAGGGAAT 
GCATTAGATA AGACTGACTG TTTGCAGCGG CGGGTAGATG GTGATTCTGT GGCTTTCAGT 
GCCCCTTCCT GTGGAAACCA GCGGGAGGAA GCCGATATAG AGCTCTGCAT AATCAGTGCC 
AAGCAGGGGG TTGCTTCACA GCCTGATAAA GGGAAGCAAG AAGAGCAAGA GGCTTGCCAG 
AAGAGGTCAG AGACTGAAAA GGACTATAAC ACTGAATCTG GCAACCTTGG GCTTCAAGAC 
ATGGGCCACG CACAATCTCA GGGTCTCCTC CCCACGGAAA CTGGTCAGCC TACACAAAGG 
GAGGTGGAGA GTGAGGGGAG TACAGATGTC TGTTGGCGTG GGGACTTACC AGAGGAAAGG 
GAAGGGGACA TGAGTAGCAA CCAGGAAATA GACTCATATT CTTCAGCACT TTCTTCCCAT 
AATCTAGAGG GAAACAGCCA GCACGATGGT GAGACTGAGC CCCAGTTGCC TACACAATGC 
TCAGAGGGAA CAGATGAAGC TAAATTGACT TTAATTCAGG TAAATTCTGA AGTTACTTTG 
TCTATACGAA GCGAGGATAC CGTTATTTCT GACAGTGTTG ACAAGCCACA TGGTCTCTTA 
GAGACGGAGA CAGGAAATCT CAGCGAACAC ACCAAAGGCA ACACTGAGTC AGCTGCTGAT 
TTGGTTGCGG ATGACGCTAG TGTAAAGCAG AACTCTGGAT TTAGCGTTTT AGAGGGGGAT 
CTTTTGGAAT CATTAAGTCT ACGGCCAAAC ATGGCACCGG GACCAGTGCA TAAGGTTCAG 
CAAGGTCTGT CACCAGTTCC TGAGACTTCC TCTCATGTAG GTCCAGCTTT GGACCCCTTT 
TCTGAGACTA CAGAAAGTTT TCAACAGCCG AATCAATCCA CACAGCTTTT AGAAACTGCA 
TTAGAATGTG AGGATGTTTC ATTAAATAAC TCAACCGGTG ACATTAATGC AATAGCAGGG 
GTTTCTGTTG ACATTTCTGC AGAGGTTTCA CTTTGCCAGA ATGAAGGGTG TCCTCCTGAT 
GGAAGCAAAG AAGAGGTAGC CCCCAGTGAG AGGATACCAG ACCTTAAAGA GCTTACGGAA 
GAGCCGGAAT CAGTTGAGGA CATTATTGTC TCAGATGTCT CAGATTTGGA TGACAAATCT 
CAACCTTTTG AAGAGTCTTC TTTGCCTATC GACTCTGAAT GTTTTATAAG AGGTGATTTC 
CAAAACGATG AGTCTACAGT ACAGTCTTCT GAATGTACTG GAGGTGACAT AAATGTAGGA 
ACACTCTTAG GAGAAGAAGA GGCAACTGAA AAAATAGAAC AGCCAGACTC TTCACTTAAA 
CTGGTTGAGC TAATTCCTGA GGAATATACT GGAGATGCCA CAAACGAAAA TAAAAAAATT 
GAGCCAGAGG AACAATCTAT TTTACTGCCA ACTTTGCAAG GGGAATCAGG ATTTAAGGAT 
GTTGCCACAG AGGAACCTGT ACAGGAAAGG GAAATTGACT CCACTTCAGA GGAAACAATC 
AGCGATCTCC CTGATCCATC GGAAATCACA AACAATGATT GTGCTGAATC CAATGCACAC 
TGCCAGCTCT CAGAATCCTC CAAGGAGAGC GCAAACACAT CCTCAGAACC TGCTGAATGT 
GTGAATGACC TAGACTTGTC TTGTGCTTTA CCTCAAACAG AAATCGAAGG CAGCAGACCT 
TCTGAGGGCC CCTTAATTGA TGGAGTAACA ATATGTGCTG AAATTCCCAT TGATGACAAA 
GGTGTTGACA TGGCTACCAA TATGAGTAAG ACGACCATCT CTGAGGATTG TCTGTCCTCT 
CCTATGGACA CCACCAGCTG TTCAGTTCTG GAGAGCTCCT CCATGTCTGA CCCACTCAAT 
GCCTCATCCT CAACAGCTGA GCTCACTTCC CCTTTGGATA TTAATAGTGG ATTGCATCAG 
GATTTGGAAC ACACTTCCCC AGTGGATATA GACAATGGTT TGCCTGAGGA ACCGCCGCCG 
TCAGACACAC CTGATTGCCT GAATGGATTA CATCAAGATT CTCCTGATAG TTTGATGGAG 
CACAGTGGAA CTCCTCAGCC ATGTTCTGAG GAACAGTGTG TGGATCAAAT CACAGATGAC 
AGCACTTCAG CAGAGGATGC AGCGGACGGA CACCAAGACT CCTCAGTAGA CATAGCAAAT 
TCCGAAGCGA TCCACAGAGA GACCGAAACA TTTTATTCAG TTGATGCCAG TCAAGACTCT 
GTACTCCAGG AGAAGAAATC TTCTTCAGTG AGCTCAGAAA TCCCCTCTTT GACCCCCTCA 
CCTGAGAGCA TACGTCACAA AGAGTCGCCA AGCACTATGC GAGGGTCCGC CAGCGATGGA 
GACTCTCTGT TTAGTCAGGA TCTGGGCGAG GACAGCGTTT TTAATAATAA GGCTGAGGAC 
AGCATTACCA TCACCAGTGG CGTGTCCATG TCCTATTCGT CCTCCACAGA TGACTCTTCC 
AGCTTGGGAA CCACTTGCGC CACCCCTCAG ACTGGCACAG AGGCTCCTGC ATCAGACCCC 
TTTCAGGAAC AGCCCACATC TACTGGAGCT TCTGGGGAAA CAGAGGAGGA AGAGAAAAAG 
GACCGTCTCA CTGAGGTTCC GGAGCGATCG GCCATTTTAC GGACCACCAT ACGATCACTG 
TCCCCTTTTC GAAGACACAG TTGGGGGCCG GGCAAAAATT TGGCAGGGGA GACAGAGATG 
AGTCAGCGCA GTTACAGTCT GGAAGGACTT GCACCAGAGA AGGAGGGAAG GAAAAGTCTG 
GTTCAGCAGC GTGTCGGAGA GTCCAGCCAG GAGCCCAAAT GTCCATCAAA GGTGGAAAAA 
GAAGACAGAG GCTCGCTTGT GTCTCTGACT GAAGAAGAGC AGGAGTCAGA TCTAGGCGAC 
TGCAGCAGTT TGGGCAGTCA GAAGTCCATC CAGTCGGGTC GGAGATGTGT CCCACATTCA 
CAACCCATCC TCACCAAATC TGTGTCCATG GTGGCCATCA GTCAGAAGGA CCTGGACGCA 
ATGGGACGGC TGCGACCCAA GCGACGAATC TCCTTCTCCT TCAGCATCTC GCCGATGCTC 
CCCAAATCTA AAACTCTCTT TTCTCTTGGC TCTTCATCCA GTGATGAGGA GGAGGCAGTC 
AGATTGAGTT CATTCACTGG AACATCAGGA TCATTAGAGT ACAGTATATC AGAGGAGGAT 
CCTGGGCCAT TACGGAGTGA CAGTGAAGGC AAAATTGGAG GAACCAAAGT GAGCAGGACA 
TTCAGTTACC TCAAGAGCAA GATGAGCAAG AAGAACAAGC AGGAGAAGGA GAAAGAGAAG 
AATAAGGAGC GTGAGCGAGA TGCCAAAGAC AAGGAGAGAA GAGCCTCAAA CGGACATCTC 
TTCACTACGG TCATGACTGT ACCCACAATC CCCTGCCACC AGTGCAACAA ACTCATCAAC 
ACTAAAGACT CTATCCTCTG CACCAATTGC AATGCACAAG TCCACAAAGG CTGCAAAGAG 
AGTCTTCCTG TCTGCGCTAA AGTCAAGATG AAGCAGCAAA AACAGCAGCA GACTGTTACA 
GATTCTGCCT CGACGCCTAG TGTCACCCTC AGAAGTAAAA CTCTTCCAGC AAGGGATCGT 
CCGTGGTCCA CAATATCCAT TCCCGATGAC CAGCCGGCTC CCCTCGCTCC TCCTCGGAAA 
AGCCCCAGCA GCATCATGTC TTTCAACAGC AACCCACTGT CCAAGAGCAT GTCCATTAAT 
AACATAGCAG GACAAATGGA CGACACTCCT CTTAAGAAGT TTCTGTCCCA GTCAACGGAC 
TCTCTTCACA AGACCAGTAA AGTCACGGAG TCTACAGAAT CTCTTACTGA TGAGGGTACA 
GAGATGATGG ACAGCCAGCT GATGGGAGAA TTTGAAGCTG ACGCCAAAGA GCTGGAGGCT 
GATTCTTATA GCTTCACGGT AGACAAGAAA TTTCTTAAGC AGCTTAAAAA GGATGTTATC 
AAAAGGCAAG ACGTCATCTA TGAGCTCATC CAGACAGAAA TGCACCATGT AAGGACCTTA 
AAGATCATGG CTGATGTTTA CAGCAAAGGA CTACTGAGAG AAGTTCAACT AGAAGCTCAG 
ACGGTGGAGA AGATGTTCCC CATGTTGGAC GATGTTCTGG ATTTGCACAC ACAGTTCTTT 
TCACACCTGC TTGAAAGAAA GAAGGAATCA GGGAGCCAAG AAGAGGGCGG CTTTGTCATC 
CAAAGGATTG GCGATGTGCT GGTTTCTCAG TTCTCAGGTT CCAGTGCTGA GAGAATGAAG 
AAGGTCTATG GGAAGTTCTG CGGCAGGCAC AATGAAGCTG TGAACTTCTA CAAGGAGCTT 
CACACCAAAG ACAAACGCTT TCAAGCTTTC ATCAAGAAAA AGATGAGCAG CCCTGTTGTC 
CGACGGCTAA GCATTCCTGA GTGTATATTG TTAGTCACAC AACGAATCAC AAAATATCCC 
GTGTTACTAC AGAGAATACT GCAGCACACG AAAGAAAACG AGGAGGACCA TGCAGACGTT 
AGTCAAGGTC TGAAGCTGGT GAAGGAGGTG ATTGCTGCCG TGGACAATAA AGTGAATGAG 
CATGAGAAGA AGAAGAGGCT AAAGGAAGTG TACAGCCGGA CGGACAACAA GTCCATCATG 
AGGATGAAGA GCGGGCAGAT GTTTGCCAGA GAGGATCTGT TACGTGGGCG GAAGTTAATT 
CACGACGGGC CGCTGCAGCT CAAGAACTCA GCTGGCAGAC TCAAGGATGT CCAGGCTCTG 
CTGCTGAGGG ACGTGATTGT GTTCCTGCAG GAGAAAGATC AGAAGTACAT TTTCGCCTCT 
TTGGACCAGC GCTCCACCGT CATCTCCCTG CAGAAGCTCA TTGTGAGGGA AGTAGCCAAT 
GAGGAGAAAG CTCTGTTTCT GATCACAGCA GGCATCGAGA AGCCCGAGAT GGTAGAAGTG 
TACACAAGCT CCAAAGAGGA GAGAAACACA TGGATGCAGC TCATTCAGGA GGCCATGCAG 
TCTATACTGT CCATTTCCTC CAGGGAGAGA GATGATGATG AAGGTGTGCC AAGTGAAAAC 
GAAGAGGACA GGAGACTTCA GGAAATCAAG GTCAAGGAGT TCAGAGATCA GCTGCACAAA 
AGAGACGAGC AGATCTTGAC GTTACTGGAG GAGAAGGTGA AGTTGTTCAG GGACATGTGT 
GACTGTGGTG CACCGGACGA GACGGGGCTT CGTAATCGAA TGCTCTTCAG GGCCACGACG 
GATGATGTCA CCAGAGGAGA GCCAATCATG AAGGAGGCTC TGAAGGAAGT TGAAACGCTG 
CAGGTTCTGG TTAATGACAG TCTTGGCGGT GCGGTTCAGG AAGGTGTGTG CGCGGCTGGA 
CATGTGACTT TGCCACGACG AGCGGAAACA TTTGGAGGTT TCGACAGTCA CCAGATGAAC 
ATCTCTAAGA GCAAAGATGG AGACAGAGAG GAGCTGGAGG AGTCTGCAGA GCTCCGCAGG 
ACTGAGTCAG ACAGTGTGTT GAAGAAGACG AGTAAGACCA ACCCTTTACT GCTGGTCAGG 
AGAAACAATG AGCAGGTTCT TCAGAGCGTT GCTCATCTTC ATGACCTGCT GAACACATTA 
CAAGCTGTGG TGGTCCAGCA GGACACCTTC ATCGAAGACC AGCGTCACGC TCATCCCTCC 
TCACGCCACC CATCCATCTC CTCCTCTACC TCCTCTTTAT CATCCACCTC ATCCCGGCCG 
AGCTCTCTAA TCGAGCAGGA GAAACAGCGC AGCCTGGAGA AGCAGCGGCA GGAGGTGGCC 
AGCCTCCAAA AACAGCAGGC GGCCCACGCC GAGGAGAGGC GGCGCAGGGA AAAGCAGTGG 
GAGACCCGAG AGAAAGAGCT AACCCAGAGG GAGATGCAGA TCCAGAGCCA AGAAGAGGAG 
GCGCAGAGGA GAGCCAGACA GCTGGAGGAG GAGAAACAGG AGCTGCAGAG TAAGAAAGAG 
GAGTATCAGA GAGACCTGGC ACGACTGCGG GACAGCCAGA AGAGACTGGA GAGAGAGAGA 
GAGCAGGTGC AGAGAGAGGC AGATGAGCTT AAACTGACTG AGGAAGTGAC AAAGAACCGG 
TCTCCGTCAA CCACGTCTGA AGACTCCACG AAGTTCCAGA GCACCGGTTC ATTAGACCGT 
GACCCATCAG AGATGGAGCT CTCCTCCTCC CCGTCAGCCA GAGACTTCCT GTCCCGCATG 
GACTCCAAAC GCAAAGGGAA AAACCTCAAT CCATTCTCAT CCAACTCAAG CCAGAAGAGC 
CAGAACAGCG AGGCTCAGAG CCAGATGCCC AGCCGTCTGC TCCAGCTGGC CAAACCCAAG 
GAGAAGAAGG ACAAGAAGAA GAAGAAGGGG AAAGGCCAGC AGAGCCAGAC TGCAGACTCT 
CAGAGCACTG TTGTTCCAGG CGCTACCCCA GACGGAGAGA TCTTTTTCTG CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

wu:fi15d04 ( XM_021470637.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_021326312.1 Danio rerio wu:fi15d04 (wu:fi15d04), transcript variant X9, mRNA.

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CloneID	ODa141432
Clone ID Related Accession (Same CDS sequence)	XM_021470637.1
Accession Version	XM_021470637.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7539bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	A-kinase anchor protein 13 isoform X9
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007136.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAACTGA ATCCACAGCA AGCCCCATTA TATGGAGAGT GTCTGCTGAC GGTCCAGCTC 
TGTGAGGATG AGTGTGTTGA GGATGAGGAT GATGTGGAGT TTTACCTGCT CTTTGCTGGA 
TCCACACAGA GACATGTGTC CAGCACACTG AAGATCAGCC ATGTCACGCT GCAGGCCGTC 
TGTCCAGCTC ATGATCGCAG TGAAACGGTA CGGGTCACAC TATGCCAGGC TCGTCCCGGC 
GGCTCAGTGG ACCCAGTGTC CGAGGAGTGT TTCCAGTTTG TCCAGGACCT GGCTCTGGAC 
ATGGCCCAGT TCCTGGTAAA TGCGGCGGGG AAAACAGAAG GAGCGCTGCT GCTGGACGAG 
TGTCAGATCC CACTGAAAGA GTGCGAGCGG CTGGACGATA CTCTTGCTCT GGCTCTCCGG 
CATCTGCCTT TGCCTGCTGG ATGGAGCGTT TTGGGCACAG ATTTGGACAC GGGCACTTGC 
ACAGGCTCGG GCATCGCAGA GCCTGGACCA CACGATACAC TTTTGCACTT CGCTGCGAAG 
CGCGGACTAC GCAAGGTTGC ACTCTTTCTG CTCCAGCAAC CAGGGGGAAG AGAGGCTCTC 
CGGCTTCCCA ACAAACAGGG TCGCACACCG GCCAAGGTCG CCCAGAAGAG GGGACACTCT 
CAGCTCCACA AACTGCTCAC GGAGGTTGAC AATCTGCCCG AGCTGGAGAC GAAAACATCT 
AAGTGGTGTT ACCCAGAAGG CAGAGTCCTG CAACACCACC CCAAGCTAAA CACTTACACC 
CTTACCCTGG AGGATGTGCC CAGGAGCTCC CCACCAAGCC TTCAGTGTGA TGTGGAGGAG 
CTCCAGCGTC TCATTCAGTC ACATCAGAAG GAAGATCCTC CAACACCGCA TAAGTCAGCA 
CTGCTGATTC TCAATAGAGA CTCCAGTAAT AGCACAGAGA CAGATGACTG CCTTCCGCCC 
AACTCAGTGG AACCTGAGCA TCAGGAGCAC AACAAACAAG AAGAAACTTG CAAATCGCTT 
TTAAACAGCC TTACCTTTGA GGATGGATTG TGTGTTCATC AAAACGGCAC AGGAGATCAT 
GTCCAGTTGC TGGATGGGGA CGTGGAGCCT GACCTGCCAG CTGTAGGAGA TACAGGGAAT 
GCATTAGATA AGACTGACTG TTTGCAGCGG CGGGTAGATG GTGATTCTGT GGCTTTCAGT 
GCCCCTTCCT GTGGAAACCA GCGGGAGGAA GCCGATATAG AGCTCTGCAT AATCAGTGCC 
AAGCAGGGGG TTGCTTCACA GCCTGATAAA GGGAAGCAAG AAGAGCAAGA GGCTTGCCAG 
AAGAGGTCAG AGACTGAAAA GGACTATAAC ACTGAATCTG GCAACCTTGG GCTTCAAGAC 
ATGGGCCACG CACAATCTCA GGGTCTCCTC CCCACGGAAA CTGGTCAGCC TACACAAAGG 
GAGGTGGAGA GTGAGGGGAG TACAGATGTC TGTTGGCGTG GGGACTTACC AGAGGAAAGG 
GAAGGGGACA TGAGTAGCAA CCAGGAAATA GACTCATATT CTTCAGCACT TTCTTCCCAT 
AATCTAGAGG GAAACAGCCA GCACGATGGT GAGACTGAGC CCCAGTTGCC TACACAATGC 
TCAGAGGGAA CAGATGAAGC TAAATTGACT TTAATTCAGG TAAATTCTGA AGTTACTTTG 
TCTATACGAA GCGAGGATAC CGTTATTTCT GACAGTGTTG ACAAGCCACA TGGTCTCTTA 
GAGACGGAGA CAGGAAATCT CAGCGAACAC ACCAAAGGCA ACACTGAGTC AGCTGCTGAT 
TTGGTTGCGG ATGACGCTAG TGTAAAGCAG AACTCTGGAT TTAGCGTTTT AGAGGGGGAT 
CTTTTGGAAT CATTAAGTCT ACGGCCAAAC ATGGCACCGG GACCAGTGCA TAAGGTTCAG 
CAAGGTCTGT CACCAGTTCC TGAGACTTCC TCTCATGTAG GTCCAGCTTT GGACCCCTTT 
TCTGAGACTA CAGAAAGTTT TCAACAGCCG AATCAATCCA CACAGCTTTT AGAAACTGCA 
TTAGAATGTG AGGATGTTTC ATTAAATAAC TCAACCGGTG ACATTAATGC AATAGCAGGG 
GTTTCTGTTG ACATTTCTGC AGAGGTTTCA CTTTGCCAGA ATGAAGGGTG TCCTCCTGAT 
GGAAGCAAAG AAGAGGTAGC CCCCAGTGAG AGGATACCAG ACCTTAAAGA GCTTACGGAA 
GAGCCGGAAT CAGTTGAGGA CATTATTGTC TCAGATGTCT CAGATTTGGA TGACAAATCT 
CAACCTTTTG AAGAGTCTTC TTTGCCTATC GACTCTGAAT GTTTTATAAG AGGTGATTTC 
CAAAACGATG AGTCTACAGT ACAGTCTTCT GAATGTACTG GAGGTGACAT AAATGTAGGA 
ACACTCTTAG GAGAAGAAGA GGCAACTGAA AAAATAGAAC AGCCAGACTC TTCACTTAAA 
CTGGTTGAGC TAATTCCTGA GGAATATACT GGAGATGCCA CAAACGAAAA TAAAAAAATT 
GAGCCAGAGG AACAATCTAT TTTACTGCCA ACTTTGCAAG GGGAATCAGG ATTTAAGGAT 
GTTGCCACAG AGGAACCTGT ACAGGAAAGG GAAATTGACT CCACTTCAGA GGAAACAATC 
AGCGATCTCC CTGATCCATC GGAAATCACA AACAATGATT GTGCTGAATC CAATGCACAC 
TGCCAGCTCT CAGAATCCTC CAAGGAGAGC GCAAACACAT CCTCAGAACC TGCTGAATGT 
GTGAATGACC TAGACTTGTC TTGTGCTTTA CCTCAAACAG AAATCGAAGG CAGCAGACCT 
TCTGAGGGCC CCTTAATTGA TGGAGTAACA ATATGTGCTG AAATTCCCAT TGATGACAAA 
GGTGTTGACA TGGCTACCAA TATGAGTAAG ACGACCATCT CTGAGGATTG TCTGTCCTCT 
CCTATGGACA CCACCAGCTG TTCAGTTCTG GAGAGCTCCT CCATGTCTGA CCCACTCAAT 
GCCTCATCCT CAACAGCTGA GCTCACTTCC CCTTTGGATA TTAATAGTGG ATTGCATCAG 
GATTTGGAAC ACACTTCCCC AGTGGATATA GACAATGGTT TGCCTGAGGA ACCGCCGCCG 
TCAGACACAC CTGATTGCCT GAATGGATTA CATCAAGATT CTCCTGATAG TTTGATGGAG 
CACAGTGGAA CTCCTCAGCC ATGTTCTGAG GAACAGTGTG TGGATCAAAT CACAGATGAC 
AGCACTTCAG CAGAGGATGC AGCGGACGGA CACCAAGACT CCTCAGTAGA CATAGCAAAT 
TCCGAAGCGA TCCACAGAGA GACCGAAACA TTTTATTCAG TTGATGCCAG TCAAGACTCT 
GTACTCCAGG AGAAGAAATC TTCTTCAGTG AGCTCAGAAA TCCCCTCTTT GACCCCCTCA 
CCTGAGAGCA TACGTCACAA AGAGTCGCCA AGCACTATGC GAGGGTCCGC CAGCGATGGA 
GACTCTCTGT TTAGTCAGGA TCTGGGCGAG GACAGCGTTT TTAATAATAA GGCTGAGGAC 
AGCATTACCA TCACCAGTGG CGTGTCCATG TCCTATTCGT CCTCCACAGA TGACTCTTCC 
AGCTTGGGAA CCACTTGCGC CACCCCTCAG ACTGGCACAG AGGCTCCTGC ATCAGACCCC 
TTTCAGGAAC AGCCCACATC TACTGGAGCT TCTGGGGAAA CAGAGGAGGA AGAGAAAAAG 
GACCGTCTCA CTGAGGTTCC GGAGCGATCG GCCATTTTAC GGACCACCAT ACGATCACTG 
TCCCCTTTTC GAAGACACAG TTGGGGGCCG GGCAAAAATT TGGCAGGGGA GACAGAGATG 
AGTCAGCGCA GTGCGGCACA AGGTGAAGTG GAGCGCAGAT CTGTTGGTCA TAGGAGAAGC 
ATGAGCTGGT GTCCTTCTGT TGTCCTGCGC TCTGAAAATG ATGAAATGAA TGAAAGAAGT 
TACAGTCTGG AAGGACTTGC ACCAGAGAAG GAGGGAAGGA AAAGTCTGGT TCAGCAGCGT 
GTCGGAGAGT CCAGCCAGGA GCCCAAATGT CCATCAAAGG TGGAAAAAGA AGACAGAGGC 
TCGCTTGTGT CTCTGACTGA AGAAGAGCAG GAGTCAGATC TAGGCGACTG CAGCAGTTTG 
GGCAGTCAGA AGTCCATCCA GTCGGGTCGG AGATGTGTCC CACATTCACA ACCCATCCTC 
ACCAAATCTG TGTCCATGGT GGCCATCAGT CAGAAGGACC TGGACGGATT GAGTTCATTC 
ACTGGAACAT CAGGATCATT AGAGTACAGT ATATCAGAGG AGGATCCTGG GCCATTACGG 
AGTGACAGTG AAGGCAAAAT TGGAGGAACC AAAGTGAGCA GGACATTCAG TTACCTCAAG 
AGCAAGATGA GCAAGAAGAA CAAGCAGGAG AAGGAGAAAG AGAAGAATAA GGAGCGTGAG 
CGAGATGCCA AAGACAAGGA GAGAAGAGCC TCAAACGGAC ATCTCTTCAC TACGGTCATG 
ACTGTACCCA CAATCCCCTG CCACCAGTGC AACAAACTCA TCAACACTAA AGACTCTATC 
CTCTGCACCA ATTGCAATGC ACAAGTCCAC AAAGGCTGCA AAGAGAGTCT TCCTGTCTGC 
GCTAAAGTCA AGATGAAGCA GCAAAAACAG CAGCAGACTG TTACAGATTC TGCCTCGACG 
CCTAGTGTCA CCCTCAGAAG TAAAACTCTT CCAGCAAGGG ATCGTCCGTG GTCCACAATA 
TCCATTCCCG ATGACCAGCC GGCTCCCCTC GCTCCTCCTC GGAAAAGCCC CAGCAGCATC 
ATGTCTTTCA ACAGCAACCC ACTGTCCAAG AGCATGTCCA TTAATAACAT AGCAGGACAA 
ATGGACGACA CTCCTCTTAA GAAGTTTCTG TCCCAGTCAA CGGACTCTCT TCACAAGACC 
AGTAAAGTCA CGGAGTCTAC AGAATCTCTT ACTGATGAGG GTACAGAGAT GATGGACAGC 
CAGCTGATGG GAGAATTTGA AGCTGACGCC AAAGAGCTGG AGGCTGATTC TTATAGCTTC 
ACGGTAGACA AGAAATTTCT TAAGCAGCTT AAAAAGGATG TTATCAAAAG GCAAGACGTC 
ATCTATGAGC TCATCCAGAC AGAAATGCAC CATGTAAGGA CCTTAAAGAT CATGGCTGAT 
GTTTACAGCA AAGGACTACT GAGAGAAGTT CAACTAGAAG CTCAGACGGT GGAGAAGATG 
TTCCCCATGT TGGACGATGT TCTGGATTTG CACACACAGT TCTTTTCACA CCTGCTTGAA 
AGAAAGAAGG AATCAGGGAG CCAAGAAGAG GGCGGCTTTG TCATCCAAAG GATTGGCGAT 
GTGCTGGTTT CTCAGTTCTC AGGTTCCAGT GCTGAGAGAA TGAAGAAGGT CTATGGGAAG 
TTCTGCGGCA GGCACAATGA AGCTGTGAAC TTCTACAAGG AGCTTCACAC CAAAGACAAA 
CGCTTTCAAG CTTTCATCAA GAAAAAGATG AGCAGCCCTG TTGTCCGACG GCTAAGCATT 
CCTGAGTGTA TATTGTTAGT CACACAACGA ATCACAAAAT ATCCCGTGTT ACTACAGAGA 
ATACTGCAGC ACACGAAAGA AAACGAGGAG GACCATGCAG ACGTTAGTCA AGGTCTGAAG 
CTGGTGAAGG AGGTGATTGC TGCCGTGGAC AATAAAGTGA ATGAGCATGA GAAGAAGAAG 
AGGCTAAAGG AAGTGTACAG CCGGACGGAC AACAAGTCCA TCATGAGGAT GAAGAGCGGG 
CAGATGTTTG CCAGAGAGGA TCTGTTACGT GGGCGGAAGT TAATTCACGA CGGGCCGCTG 
CAGCTCAAGA ACTCAGCTGG CAGACTCAAG GATGTCCAGG CTCTGCTGCT GAGGGACGTG 
ATTGTGTTCC TGCAGGAGAA AGATCAGAAG TACATTTTCG CCTCTTTGGA CCAGCGCTCC 
ACCGTCATCT CCCTGCAGAA GCTCATTGTG AGGGAAGTAG CCAATGAGGA GAAAGCTCTG 
TTTCTGATCA CAGCAGGCAT CGAGAAGCCC GAGATGGTAG AAGTGTACAC AAGCTCCAAA 
GAGGAGAGAA ACACATGGAT GCAGCTCATT CAGGAGGCCA TGCAGTCTAT ACTGTCCATT 
TCCTCCAGGG AGAGAGATGA TGATGAAGGT GTGCCAAGTG AAAACGAAGA GGACAGGAGA 
CTTCAGGAAA TCAAGGTCAA GGAGTTCAGA GATCAGCTGC ACAAAAGAGA CGAGCAGATC 
TTGACGTTAC TGGAGGAGAA GGTGAAGTTG TTCAGGGACA TGTGTGACTG TGGTGCACCG 
GACGAGACGG GGCTTCGTAA TCGAATGCTC TTCAGGGCCA CGACGGATGA TGTCACCAGA 
GGAGAGCCAA TCATGAAGGA GGCTCTGAAG GAAGTTGAAA CGCTGCAGGT TCTGGTTAAT 
GACAGTCTTG GCGGTGCGGT TCAGGAAGGT GTGTGCGCGG CTGGACATGT GACTTTGCCA 
CGACGAGCGG AAACATTTGG AGGTTTCGAC AGTCACCAGA TGAACATCTC TAAGAGCAAA 
GATGGAGACA GAGAGGAGCT GGAGGAGTCT GCAGAGCTCC GCAGGACTGA GTCAGACAGT 
GTGTTGAAGA AGACGAGTAA GACCAACCCT TTACTGCTGG TCAGGAGAAA CAATGAGCAG 
GTTCTTCAGA GCGTTGCTCA TCTTCATGAC CTGCTGAACA CATTACAAGC TGTGGTGGTC 
CAGCAGGACA CCTTCATCGA AGACCAGCGT CACGCTCATC CCTCCTCACG CCACCCATCC 
ATCTCCTCCT CTACCTCCTC TTTATCATCC ACCTCATCCC GGCCGAGCTC TCTAATCGAG 
CAGGAGAAAC AGCGCAGCCT GGAGAAGCAG CGGCAGGAGG TGGCCAGCCT CCAAAAACAG 
CAGGCGGCCC ACGCCGAGGA GAGGCGGCGC AGGGAAAAGC AGTGGGAGAC CCGAGAGAAA 
GAGCTAACCC AGAGGGAGAT GCAGATCCAG AGCCAAGAAG AGGAGGCGCA GAGGAGAGCC 
AGACAGCTGG AGGAGGAGAA ACAGGAGCTG CAGAGTAAGA AAGAGGAGTA TCAGAGAGAC 
CTGGCACGAC TGCGGGACAG CCAGAAGAGA CTGGAGAGAG AGAGAGAGCA GGTGCAGAGA 
GAGGCAGATG AGCTTAAACT GACTGAGGAA GTGACAAAGA ACCGGTCTCC GTCAACCACG 
TCTGAAGACT CCACGAAGTT CCAGAGCACC GGTTCATTAG ACCGTGACCC ATCAGAGATG 
GAGCTCTCCT CCTCCCCGTC AGCCAGAGAC TTCCTGTCCC GCATGGACTC CAAACGCAAA 
GGGAAAAACC TCAATCCATT CTCATCCAAC TCAAGCCAGA AGAGCCAGAA CAGCGAGGCT 
CAGAGCCAGA TGCCCAGCCG TCTGCTCCAG CTGGCCAAAC CCAAGGAGAA GAAGGACAAG 
AAGAAGAAGA AGGGGAAAGG CCAGCAGAGC CAGACTGCAG ACTCTCAGAG CACTGTTGTT 
CCAGGCGCTA CCCCAGACGG AGAGATCTTT TTCTGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_021326312.1
CDS	398..7936
Translation	MKLNPQQAPLYGECLLTVQLCEDECVEDEDDVEFYLLFAGSTQRHVSSTLKISHVTLQAVCPAHDRSETVRVTLCQARPGGSVDPVSEECFQFVQDLALDMAQFLVNAAGKTEGALLLDECQIPLKECERLDDTLALALRHLPLPAGWSVLGTDLDTGTCTGSGIAEPGPHDTLLHFAAKRGLRKVALFLLQQPGGREALRLPNKQGRTPAKVAQKRGHSQLHKLLTEVDNLPELETKTSKWCYPEGRVLQHHPKLNTYTLTLEDVPRSSPPSLQCDVEELQRLIQSHQKEDPPTPHKSALLILNRDSSNSTETDDCLPPNSVEPEHQEHNKQEETCKSLLNSLTFEDGLCVHQNGTGDHVQLLDGDVEPDLPAVGDTGNALDKTDCLQRRVDGDSVAFSAPSCGNQREEADIELCIISAKQGVASQPDKGKQEEQEACQKRSETEKDYNTESGNLGLQDMGHAQSQGLLPTETGQPTQREVESEGSTDVCWRGDLPEEREGDMSSNQEIDSYSSALSSHNLEGNSQHDGETEPQLPTQCSEGTDEAKLTLIQVNSEVTLSIRSEDTVISDSVDKPHGLLETETGNLSEHTKGNTESAADLVADDASVKQNSGFSVLEGDLLESLSLRPNMAPGPVHKVQQGLSPVPETSSHVGPALDPFSETTESFQQPNQSTQLLETALECEDVSLNNSTGDINAIAGVSVDISAEVSLCQNEGCPPDGSKEEVAPSERIPDLKELTEEPESVEDIIVSDVSDLDDKSQPFEESSLPIDSECFIRGDFQNDESTVQSSECTGGDINVGTLLGEEEATEKIEQPDSSLKLVELIPEEYTGDATNENKKIEPEEQSILLPTLQGESGFKDVATEEPVQEREIDSTSEETISDLPDPSEITNNDCAESNAHCQLSESSKESANTSSEPAECVNDLDLSCALPQTEIEGSRPSEGPLIDGVTICAEIPIDDKGVDMATNMSKTTISEDCLSSPMDTTSCSVLESSSMSDPLNASSSTAELTSPLDINSGLHQDLEHTSPVDIDNGLPEEPPPSDTPDCLNGLHQDSPDSLMEHSGTPQPCSEEQCVDQITDDSTSAEDAADGHQDSSVDIANSEAIHRETETFYSVDASQDSVLQEKKSSSVSSEIPSLTPSPESIRHKESPSTMRGSASDGDSLFSQDLGEDSVFNNKAEDSITITSGVSMSYSSSTDDSSSLGTTCATPQTGTEAPASDPFQEQPTSTGASGETEEEEKKDRLTEVPERSAILRTTIRSLSPFRRHSWGPGKNLAGETEMSQRSAAQGEVERRSVGHRRSMSWCPSVVLRSENDEMNERSYSLEGLAPEKEGRKSLVQQRVGESSQEPKCPSKVEKEDRGSLVSLTEEEQESDLGDCSSLGSQKSIQSGRRCVPHSQPILTKSVSMVAISQKDLDGLSSFTGTSGSLEYSISEEDPGPLRSDSEGKIGGTKVSRTFSYLKSKMSKKNKQEKEKEKNKERERDAKDKERRASNGHLFTTVMTVPTIPCHQCNKLINTKDSILCTNCNAQVHKGCKESLPVCAKVKMKQQKQQQTVTDSASTPSVTLRSKTLPARDRPWSTISIPDDQPAPLAPPRKSPSSIMSFNSNPLSKSMSINNIAGQMDDTPLKKFLSQSTDSLHKTSKVTESTESLTDEGTEMMDSQLMGEFEADAKELEADSYSFTVDKKFLKQLKKDVIKRQDVIYELIQTEMHHVRTLKIMADVYSKGLLREVQLEAQTVEKMFPMLDDVLDLHTQFFSHLLERKKESGSQEEGGFVIQRIGDVLVSQFSGSSAERMKKVYGKFCGRHNEAVNFYKELHTKDKRFQAFIKKKMSSPVVRRLSIPECILLVTQRITKYPVLLQRILQHTKENEEDHADVSQGLKLVKEVIAAVDNKVNEHEKKKRLKEVYSRTDNKSIMRMKSGQMFAREDLLRGRKLIHDGPLQLKNSAGRLKDVQALLLRDVIVFLQEKDQKYIFASLDQRSTVISLQKLIVREVANEEKALFLITAGIEKPEMVEVYTSSKEERNTWMQLIQEAMQSILSISSRERDDDEGVPSENEEDRRLQEIKVKEFRDQLHKRDEQILTLLEEKVKLFRDMCDCGAPDETGLRNRMLFRATTDDVTRGEPIMKEALKEVETLQVLVNDSLGGAVQEGVCAAGHVTLPRRAETFGGFDSHQMNISKSKDGDREELEESAELRRTESDSVLKKTSKTNPLLLVRRNNEQVLQSVAHLHDLLNTLQAVVVQQDTFIEDQRHAHPSSRHPSISSSTSSLSSTSSRPSSLIEQEKQRSLEKQRQEVASLQKQQAAHAEERRRREKQWETREKELTQREMQIQSQEEEAQRRARQLEEEKQELQSKKEEYQRDLARLRDSQKRLEREREQVQREADELKLTEEVTKNRSPSTTSEDSTKFQSTGSLDRDPSEMELSSSPSARDFLSRMDSKRKGKNLNPFSSNSSQKSQNSEAQSQMPSRLLQLAKPKEKKDKKKKKGKGQQSQTADSQSTVVPGATPDGEIFFC

Target ORF information:

RefSeq Version	XM_021470637.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio wu:fi15d04 (wu:fi15d04), transcript variant X9, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_021470637.1

ORF Insert Sequence:

ATGAAACTGA ATCCACAGCA AGCCCCATTA TATGGAGAGT GTCTGCTGAC GGTCCAGCTC 
TGTGAGGATG AGTGTGTTGA GGATGAGGAT GATGTGGAGT TTTACCTGCT CTTTGCTGGA 
TCCACACAGA GACATGTGTC CAGCACACTG AAGATCAGCC ATGTCACGCT GCAGGCCGTC 
TGTCCAGCTC ATGATCGCAG TGAAACGGTA CGGGTCACAC TATGCCAGGC TCGTCCCGGC 
GGCTCAGTGG ACCCAGTGTC CGAGGAGTGT TTCCAGTTTG TCCAGGACCT GGCTCTGGAC 
ATGGCCCAGT TCCTGGTAAA TGCGGCGGGG AAAACAGAAG GAGCGCTGCT GCTGGACGAG 
TGTCAGATCC CACTGAAAGA GTGCGAGCGG CTGGACGATA CTCTTGCTCT GGCTCTCCGG 
CATCTGCCTT TGCCTGCTGG ATGGAGCGTT TTGGGCACAG ATTTGGACAC GGGCACTTGC 
ACAGGCTCGG GCATCGCAGA GCCTGGACCA CACGATACAC TTTTGCACTT CGCTGCGAAG 
CGCGGACTAC GCAAGGTTGC ACTCTTTCTG CTCCAGCAAC CAGGGGGAAG AGAGGCTCTC 
CGGCTTCCCA ACAAACAGGG TCGCACACCG GCCAAGGTCG CCCAGAAGAG GGGACACTCT 
CAGCTCCACA AACTGCTCAC GGAGGTTGAC AATCTGCCCG AGCTGGAGAC GAAAACATCT 
AAGTGGTGTT ACCCAGAAGG CAGAGTCCTG CAACACCACC CCAAGCTAAA CACTTACACC 
CTTACCCTGG AGGATGTGCC CAGGAGCTCC CCACCAAGCC TTCAGTGTGA TGTGGAGGAG 
CTCCAGCGTC TCATTCAGTC ACATCAGAAG GAAGATCCTC CAACACCGCA TAAGTCAGCA 
CTGCTGATTC TCAATAGAGA CTCCAGTAAT AGCACAGAGA CAGATGACTG CCTTCCGCCC 
AACTCAGTGG AACCTGAGCA TCAGGAGCAC AACAAACAAG AAGAAACTTG CAAATCGCTT 
TTAAACAGCC TTACCTTTGA GGATGGATTG TGTGTTCATC AAAACGGCAC AGGAGATCAT 
GTCCAGTTGC TGGATGGGGA CGTGGAGCCT GACCTGCCAG CTGTAGGAGA TACAGGGAAT 
GCATTAGATA AGACTGACTG TTTGCAGCGG CGGGTAGATG GTGATTCTGT GGCTTTCAGT 
GCCCCTTCCT GTGGAAACCA GCGGGAGGAA GCCGATATAG AGCTCTGCAT AATCAGTGCC 
AAGCAGGGGG TTGCTTCACA GCCTGATAAA GGGAAGCAAG AAGAGCAAGA GGCTTGCCAG 
AAGAGGTCAG AGACTGAAAA GGACTATAAC ACTGAATCTG GCAACCTTGG GCTTCAAGAC 
ATGGGCCACG CACAATCTCA GGGTCTCCTC CCCACGGAAA CTGGTCAGCC TACACAAAGG 
GAGGTGGAGA GTGAGGGGAG TACAGATGTC TGTTGGCGTG GGGACTTACC AGAGGAAAGG 
GAAGGGGACA TGAGTAGCAA CCAGGAAATA GACTCATATT CTTCAGCACT TTCTTCCCAT 
AATCTAGAGG GAAACAGCCA GCACGATGGT GAGACTGAGC CCCAGTTGCC TACACAATGC 
TCAGAGGGAA CAGATGAAGC TAAATTGACT TTAATTCAGG TAAATTCTGA AGTTACTTTG 
TCTATACGAA GCGAGGATAC CGTTATTTCT GACAGTGTTG ACAAGCCACA TGGTCTCTTA 
GAGACGGAGA CAGGAAATCT CAGCGAACAC ACCAAAGGCA ACACTGAGTC AGCTGCTGAT 
TTGGTTGCGG ATGACGCTAG TGTAAAGCAG AACTCTGGAT TTAGCGTTTT AGAGGGGGAT 
CTTTTGGAAT CATTAAGTCT ACGGCCAAAC ATGGCACCGG GACCAGTGCA TAAGGTTCAG 
CAAGGTCTGT CACCAGTTCC TGAGACTTCC TCTCATGTAG GTCCAGCTTT GGACCCCTTT 
TCTGAGACTA CAGAAAGTTT TCAACAGCCG AATCAATCCA CACAGCTTTT AGAAACTGCA 
TTAGAATGTG AGGATGTTTC ATTAAATAAC TCAACCGGTG ACATTAATGC AATAGCAGGG 
GTTTCTGTTG ACATTTCTGC AGAGGTTTCA CTTTGCCAGA ATGAAGGGTG TCCTCCTGAT 
GGAAGCAAAG AAGAGGTAGC CCCCAGTGAG AGGATACCAG ACCTTAAAGA GCTTACGGAA 
GAGCCGGAAT CAGTTGAGGA CATTATTGTC TCAGATGTCT CAGATTTGGA TGACAAATCT 
CAACCTTTTG AAGAGTCTTC TTTGCCTATC GACTCTGAAT GTTTTATAAG AGGTGATTTC 
CAAAACGATG AGTCTACAGT ACAGTCTTCT GAATGTACTG GAGGTGACAT AAATGTAGGA 
ACACTCTTAG GAGAAGAAGA GGCAACTGAA AAAATAGAAC AGCCAGACTC TTCACTTAAA 
CTGGTTGAGC TAATTCCTGA GGAATATACT GGAGATGCCA CAAACGAAAA TAAAAAAATT 
GAGCCAGAGG AACAATCTAT TTTACTGCCA ACTTTGCAAG GGGAATCAGG ATTTAAGGAT 
GTTGCCACAG AGGAACCTGT ACAGGAAAGG GAAATTGACT CCACTTCAGA GGAAACAATC 
AGCGATCTCC CTGATCCATC GGAAATCACA AACAATGATT GTGCTGAATC CAATGCACAC 
TGCCAGCTCT CAGAATCCTC CAAGGAGAGC GCAAACACAT CCTCAGAACC TGCTGAATGT 
GTGAATGACC TAGACTTGTC TTGTGCTTTA CCTCAAACAG AAATCGAAGG CAGCAGACCT 
TCTGAGGGCC CCTTAATTGA TGGAGTAACA ATATGTGCTG AAATTCCCAT TGATGACAAA 
GGTGTTGACA TGGCTACCAA TATGAGTAAG ACGACCATCT CTGAGGATTG TCTGTCCTCT 
CCTATGGACA CCACCAGCTG TTCAGTTCTG GAGAGCTCCT CCATGTCTGA CCCACTCAAT 
GCCTCATCCT CAACAGCTGA GCTCACTTCC CCTTTGGATA TTAATAGTGG ATTGCATCAG 
GATTTGGAAC ACACTTCCCC AGTGGATATA GACAATGGTT TGCCTGAGGA ACCGCCGCCG 
TCAGACACAC CTGATTGCCT GAATGGATTA CATCAAGATT CTCCTGATAG TTTGATGGAG 
CACAGTGGAA CTCCTCAGCC ATGTTCTGAG GAACAGTGTG TGGATCAAAT CACAGATGAC 
AGCACTTCAG CAGAGGATGC AGCGGACGGA CACCAAGACT CCTCAGTAGA CATAGCAAAT 
TCCGAAGCGA TCCACAGAGA GACCGAAACA TTTTATTCAG TTGATGCCAG TCAAGACTCT 
GTACTCCAGG AGAAGAAATC TTCTTCAGTG AGCTCAGAAA TCCCCTCTTT GACCCCCTCA 
CCTGAGAGCA TACGTCACAA AGAGTCGCCA AGCACTATGC GAGGGTCCGC CAGCGATGGA 
GACTCTCTGT TTAGTCAGGA TCTGGGCGAG GACAGCGTTT TTAATAATAA GGCTGAGGAC 
AGCATTACCA TCACCAGTGG CGTGTCCATG TCCTATTCGT CCTCCACAGA TGACTCTTCC 
AGCTTGGGAA CCACTTGCGC CACCCCTCAG ACTGGCACAG AGGCTCCTGC ATCAGACCCC 
TTTCAGGAAC AGCCCACATC TACTGGAGCT TCTGGGGAAA CAGAGGAGGA AGAGAAAAAG 
GACCGTCTCA CTGAGGTTCC GGAGCGATCG GCCATTTTAC GGACCACCAT ACGATCACTG 
TCCCCTTTTC GAAGACACAG TTGGGGGCCG GGCAAAAATT TGGCAGGGGA GACAGAGATG 
AGTCAGCGCA GTGCGGCACA AGGTGAAGTG GAGCGCAGAT CTGTTGGTCA TAGGAGAAGC 
ATGAGCTGGT GTCCTTCTGT TGTCCTGCGC TCTGAAAATG ATGAAATGAA TGAAAGAAGT 
TACAGTCTGG AAGGACTTGC ACCAGAGAAG GAGGGAAGGA AAAGTCTGGT TCAGCAGCGT 
GTCGGAGAGT CCAGCCAGGA GCCCAAATGT CCATCAAAGG TGGAAAAAGA AGACAGAGGC 
TCGCTTGTGT CTCTGACTGA AGAAGAGCAG GAGTCAGATC TAGGCGACTG CAGCAGTTTG 
GGCAGTCAGA AGTCCATCCA GTCGGGTCGG AGATGTGTCC CACATTCACA ACCCATCCTC 
ACCAAATCTG TGTCCATGGT GGCCATCAGT CAGAAGGACC TGGACGGATT GAGTTCATTC 
ACTGGAACAT CAGGATCATT AGAGTACAGT ATATCAGAGG AGGATCCTGG GCCATTACGG 
AGTGACAGTG AAGGCAAAAT TGGAGGAACC AAAGTGAGCA GGACATTCAG TTACCTCAAG 
AGCAAGATGA GCAAGAAGAA CAAGCAGGAG AAGGAGAAAG AGAAGAATAA GGAGCGTGAG 
CGAGATGCCA AAGACAAGGA GAGAAGAGCC TCAAACGGAC ATCTCTTCAC TACGGTCATG 
ACTGTACCCA CAATCCCCTG CCACCAGTGC AACAAACTCA TCAACACTAA AGACTCTATC 
CTCTGCACCA ATTGCAATGC ACAAGTCCAC AAAGGCTGCA AAGAGAGTCT TCCTGTCTGC 
GCTAAAGTCA AGATGAAGCA GCAAAAACAG CAGCAGACTG TTACAGATTC TGCCTCGACG 
CCTAGTGTCA CCCTCAGAAG TAAAACTCTT CCAGCAAGGG ATCGTCCGTG GTCCACAATA 
TCCATTCCCG ATGACCAGCC GGCTCCCCTC GCTCCTCCTC GGAAAAGCCC CAGCAGCATC 
ATGTCTTTCA ACAGCAACCC ACTGTCCAAG AGCATGTCCA TTAATAACAT AGCAGGACAA 
ATGGACGACA CTCCTCTTAA GAAGTTTCTG TCCCAGTCAA CGGACTCTCT TCACAAGACC 
AGTAAAGTCA CGGAGTCTAC AGAATCTCTT ACTGATGAGG GTACAGAGAT GATGGACAGC 
CAGCTGATGG GAGAATTTGA AGCTGACGCC AAAGAGCTGG AGGCTGATTC TTATAGCTTC 
ACGGTAGACA AGAAATTTCT TAAGCAGCTT AAAAAGGATG TTATCAAAAG GCAAGACGTC 
ATCTATGAGC TCATCCAGAC AGAAATGCAC CATGTAAGGA CCTTAAAGAT CATGGCTGAT 
GTTTACAGCA AAGGACTACT GAGAGAAGTT CAACTAGAAG CTCAGACGGT GGAGAAGATG 
TTCCCCATGT TGGACGATGT TCTGGATTTG CACACACAGT TCTTTTCACA CCTGCTTGAA 
AGAAAGAAGG AATCAGGGAG CCAAGAAGAG GGCGGCTTTG TCATCCAAAG GATTGGCGAT 
GTGCTGGTTT CTCAGTTCTC AGGTTCCAGT GCTGAGAGAA TGAAGAAGGT CTATGGGAAG 
TTCTGCGGCA GGCACAATGA AGCTGTGAAC TTCTACAAGG AGCTTCACAC CAAAGACAAA 
CGCTTTCAAG CTTTCATCAA GAAAAAGATG AGCAGCCCTG TTGTCCGACG GCTAAGCATT 
CCTGAGTGTA TATTGTTAGT CACACAACGA ATCACAAAAT ATCCCGTGTT ACTACAGAGA 
ATACTGCAGC ACACGAAAGA AAACGAGGAG GACCATGCAG ACGTTAGTCA AGGTCTGAAG 
CTGGTGAAGG AGGTGATTGC TGCCGTGGAC AATAAAGTGA ATGAGCATGA GAAGAAGAAG 
AGGCTAAAGG AAGTGTACAG CCGGACGGAC AACAAGTCCA TCATGAGGAT GAAGAGCGGG 
CAGATGTTTG CCAGAGAGGA TCTGTTACGT GGGCGGAAGT TAATTCACGA CGGGCCGCTG 
CAGCTCAAGA ACTCAGCTGG CAGACTCAAG GATGTCCAGG CTCTGCTGCT GAGGGACGTG 
ATTGTGTTCC TGCAGGAGAA AGATCAGAAG TACATTTTCG CCTCTTTGGA CCAGCGCTCC 
ACCGTCATCT CCCTGCAGAA GCTCATTGTG AGGGAAGTAG CCAATGAGGA GAAAGCTCTG 
TTTCTGATCA CAGCAGGCAT CGAGAAGCCC GAGATGGTAG AAGTGTACAC AAGCTCCAAA 
GAGGAGAGAA ACACATGGAT GCAGCTCATT CAGGAGGCCA TGCAGTCTAT ACTGTCCATT 
TCCTCCAGGG AGAGAGATGA TGATGAAGGT GTGCCAAGTG AAAACGAAGA GGACAGGAGA 
CTTCAGGAAA TCAAGGTCAA GGAGTTCAGA GATCAGCTGC ACAAAAGAGA CGAGCAGATC 
TTGACGTTAC TGGAGGAGAA GGTGAAGTTG TTCAGGGACA TGTGTGACTG TGGTGCACCG 
GACGAGACGG GGCTTCGTAA TCGAATGCTC TTCAGGGCCA CGACGGATGA TGTCACCAGA 
GGAGAGCCAA TCATGAAGGA GGCTCTGAAG GAAGTTGAAA CGCTGCAGGT TCTGGTTAAT 
GACAGTCTTG GCGGTGCGGT TCAGGAAGGT GTGTGCGCGG CTGGACATGT GACTTTGCCA 
CGACGAGCGG AAACATTTGG AGGTTTCGAC AGTCACCAGA TGAACATCTC TAAGAGCAAA 
GATGGAGACA GAGAGGAGCT GGAGGAGTCT GCAGAGCTCC GCAGGACTGA GTCAGACAGT 
GTGTTGAAGA AGACGAGTAA GACCAACCCT TTACTGCTGG TCAGGAGAAA CAATGAGCAG 
GTTCTTCAGA GCGTTGCTCA TCTTCATGAC CTGCTGAACA CATTACAAGC TGTGGTGGTC 
CAGCAGGACA CCTTCATCGA AGACCAGCGT CACGCTCATC CCTCCTCACG CCACCCATCC 
ATCTCCTCCT CTACCTCCTC TTTATCATCC ACCTCATCCC GGCCGAGCTC TCTAATCGAG 
CAGGAGAAAC AGCGCAGCCT GGAGAAGCAG CGGCAGGAGG TGGCCAGCCT CCAAAAACAG 
CAGGCGGCCC ACGCCGAGGA GAGGCGGCGC AGGGAAAAGC AGTGGGAGAC CCGAGAGAAA 
GAGCTAACCC AGAGGGAGAT GCAGATCCAG AGCCAAGAAG AGGAGGCGCA GAGGAGAGCC 
AGACAGCTGG AGGAGGAGAA ACAGGAGCTG CAGAGTAAGA AAGAGGAGTA TCAGAGAGAC 
CTGGCACGAC TGCGGGACAG CCAGAAGAGA CTGGAGAGAG AGAGAGAGCA GGTGCAGAGA 
GAGGCAGATG AGCTTAAACT GACTGAGGAA GTGACAAAGA ACCGGTCTCC GTCAACCACG 
TCTGAAGACT CCACGAAGTT CCAGAGCACC GGTTCATTAG ACCGTGACCC ATCAGAGATG 
GAGCTCTCCT CCTCCCCGTC AGCCAGAGAC TTCCTGTCCC GCATGGACTC CAAACGCAAA 
GGGAAAAACC TCAATCCATT CTCATCCAAC TCAAGCCAGA AGAGCCAGAA CAGCGAGGCT 
CAGAGCCAGA TGCCCAGCCG TCTGCTCCAG CTGGCCAAAC CCAAGGAGAA GAAGGACAAG 
AAGAAGAAGA AGGGGAAAGG CCAGCAGAGC CAGACTGCAG ACTCTCAGAG CACTGTTGTT 
CCAGGCGCTA CCCCAGACGG AGAGATCTTT TTCTGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

wu:fi15d04 ( XM_021470641.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_021326316.1 Danio rerio wu:fi15d04 (wu:fi15d04), transcript variant X13, mRNA.

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CloneID	ODa141433
Clone ID Related Accession (Same CDS sequence)	XM_021470641.1
Accession Version	XM_021470641.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7533bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	A-kinase anchor protein 13 isoform X13
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007136.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAACTGA ATCCACAGCA AGCCCCATTA TATGGAGAGT GTCTGCTGAC GGTCCAGCTC 
TGTGAGGATG AGTGTGTTGA GGATGAGGAT GATGTGGAGT TTTACCTGCT CTTTGCTGGA 
TCCACACAGA GACATGTGTC CAGCACACTG AAGATCAGCC ATGTCACGCT GCAGGCCGTC 
TGTCCAGCTC ATGATCGCAG TGAAACGGTA CGGGTCACAC TATGCCAGGC TCGTCCCGGC 
GGCTCAGTGG ACCCAGTGTC CGAGGAGTGT TTCCAGTTTG TCCAGGACCT GGCTCTGGAC 
ATGGCCCAGT TCCTGGTAAA TGCGGCGGGG AAAACAGAAG GAGCGCTGCT GCTGGACGAG 
TGTCAGATCC CACTGAAAGA GTGCGAGCGG CTGGACGATA CTCTTGCTCT GGCTCTCCGG 
CATCTGCCTT TGCCTGCTGG ATGGAGCGTT TTGGGCACAG ATTTGGACAC GGGCACTTGC 
ACAGGCTCGG GCATCGCAGA GCCTGGACCA CACGATACAC TTTTGCACTT CGCTGCGAAG 
CGCGGACTAC GCAAGGTTGC ACTCTTTCTG CTCCAGCAAC CAGGGGGAAG AGAGGCTCTC 
CGGCTTCCCA ACAAACAGGG TCGCACACCG GCCAAGGTCG CCCAGAAGAG GGGACACTCT 
CAGCTCCACA AACTGCTCAC GGAGGTTGAC AATCTGCCCG AGCTGGAGAC GAAAACATCT 
AAGTGGTGTT ACCCAGAAGG CAGAGTCCTG CAACACCACC CCAAGCTAAA CACTTACACC 
CTTACCCTGG AGGATGTGCC CAGGAGCTCC CCACCAAGCC TTCAGTGTGA TGTGGAGGAG 
CTCCAGCGTC TCATTCAGTC ACATCAGAAG GAAGATCCTC CAACACCGCA TAAGTCAGCA 
CTGCTGATTC TCAATAGAGA CTCCAGTAAT AGCACAGAGA CAGATGACTG CCTTCCGCCC 
AACTCAGTGG AACCTGAGCA TCAGGAGCAC AACAAACAAG AAGAAACTTG CAAATCGCTT 
TTAAACAGCC TTACCTTTGA GGATGGATTG TGTGTTCATC AAAACGGCAC AGGAGATCAT 
GTCCAGTTGC TGGATGGGGA CGTGGAGCCT GACCTGCCAG CTGTAGGAGA TACAGGGAAT 
GCATTAGATA AGACTGACTG TTTGCAGCGG CGGGTAGATG GTGATTCTGT GGCTTTCAGT 
GCCCCTTCCT GTGGAAACCA GCGGGAGGAA GCCGATATAG AGCTCTGCAT AATCAGTGCC 
AAGCAGGGGG TTGCTTCACA GCCTGATAAA GGGAAGCAAG AAGAGCAAGA GGCTTGCCAG 
AAGAGGTCAG AGACTGAAAA GGACTATAAC ACTGAATCTG GCAACCTTGG GCTTCAAGAC 
ATGGGCCACG CACAATCTCA GGGTCTCCTC CCCACGGAAA CTGGTCAGCC TACACAAAGG 
GAGGTGGAGA GTGAGGGGAG TACAGATGTC TGTTGGCGTG GGGACTTACC AGAGGAAAGG 
GAAGGGGACA TGAGTAGCAA CCAGGAAATA GACTCATATT CTTCAGCACT TTCTTCCCAT 
AATCTAGAGG GAAACAGCCA GCACGATGGT GAGACTGAGC CCCAGTTGCC TACACAATGC 
TCAGAGGGAA CAGATGAAGC TAAATTGACT TTAATTCAGG TAAATTCTGA AGTTACTTTG 
TCTATACGAA GCGAGGATAC CGTTATTTCT GACAGTGTTG ACAAGCCACA TGGTCTCTTA 
GAGACGGAGA CAGGAAATCT CAGCGAACAC ACCAAAGGCA ACACTGAGTC AGCTGCTGAT 
TTGGTTGCGG ATGACGCTAG TGTAAAGCAG AACTCTGGAT TTAGCGTTTT AGAGGGGGAT 
CTTTTGGAAT CATTAAGTCT ACGGCCAAAC ATGGCACCGG GACCAGTGCA TAAGGTTCAG 
CAAGGTCTGT CACCAGTTCC TGAGACTTCC TCTCATGTAG GTCCAGCTTT GGACCCCTTT 
TCTGAGACTA CAGAAAGTTT TCAACAGCCG AATCAATCCA CACAGCTTTT AGAAACTGCA 
TTAGAATGTG AGGATGTTTC ATTAAATAAC TCAACCGGTG ACATTAATGC AATAGCAGGG 
GTTTCTGTTG ACATTTCTGC AGAGGTTTCA CTTTGCCAGA ATGAAGGGTG TCCTCCTGAT 
GGAAGCAAAG AAGAGGTAGC CCCCAGTGAG AGGATACCAG ACCTTAAAGA GCTTACGGAA 
GAGCCGGAAT CAGTTGAGGA CATTATTGTC TCAGATGTCT CAGATTTGGA TGACAAATCT 
CAACCTTTTG AAGAGTCTTC TTTGCCTATC GACTCTGAAT GTTTTATAAG AGGTGATTTC 
CAAAACGATG AGTCTACAGT ACAGTCTTCT GAATGTACTG GAGGTGACAT AAATGTAGGA 
ACACTCTTAG GAGAAGAAGA GGCAACTGAA AAAATAGAAC AGCCAGACTC TTCACTTAAA 
CTGGTTGAGC TAATTCCTGA GGAATATACT GGAGATGCCA CAAACGAAAA TAAAAAAATT 
GAGCCAGAGG AACAATCTAT TTTACTGCCA ACTTTGCAAG GGGAATCAGG ATTTAAGGAT 
GTTGCCACAG AGGAACCTGT ACAGGAAAGG GAAATTGACT CCACTTCAGA GGAAACAATC 
AGCGATCTCC CTGATCCATC GGAAATCACA AACAATGATT GTGCTGAATC CAATGCACAC 
TGCCAGCTCT CAGAATCCTC CAAGGAGAGC GCAAACACAT CCTCAGAACC TGCTGAATGT 
GTGAATGACC TAGACTTGTC TTGTGCTTTA CCTCAAACAG AAATCGAAGG CAGCAGACCT 
TCTGAGGGCC CCTTAATTGA TGGAGTAACA ATATGTGCTG AAATTCCCAT TGATGACAAA 
GGTGTTGACA TGGCTACCAA TATGAGTAAG ACGACCATCT CTGAGGATTG TCTGTCCTCT 
CCTATGGACA CCACCAGCTG TTCAGTTCTG GAGAGCTCCT CCATGTCTGA CCCACTCAAT 
GCCTCATCCT CAACAGCTGA GCTCACTTCC CCTTTGGATA TTAATAGTGG ATTGCATCAG 
GATTTGGAAC ACACTTCCCC AGTGGATATA GACAATGGTT TGCCTGAGGA ACCGCCGCCG 
TCAGACACAC CTGATTGCCT GAATGGATTA CATCAAGATT CTCCTGATAG TTTGATGGAG 
CACAGTGGAA CTCCTCAGCC ATGTTCTGAG GAACAGTGTG TGGATCAAAT CACAGATGAC 
AGCACTTCAG CAGAGGATGC AGCGGACGGA CACCAAGACT CCTCAGTAGA CATAGCAAAT 
TCCGAAGCGA TCCACAGAGA GACCGAAACA TTTTATTCAG TTGATGCCAG TCAAGACTCT 
GTACTCCAGG AGAAGAAATC TTCTTCAGTG AGCTCAGAAA TCCCCTCTTT GACCCCCTCA 
CCTGAGAGCA TACGTCACAA AGAGTCGCCA AGCACTATGC GAGGGTCCGC CAGCGATGGA 
GACTCTCTGT TTAGTCAGGA TCTGGGCGAG GACAGCGTTT TTAATAATAA GGCTGAGGAC 
AGCATTACCA TCACCAGTGG CGTGTCCATG TCCTATTCGT CCTCCACAGA TGACTCTTCC 
AGCTTGGGAA CCACTTGCGC CACCCCTCAG ACTGGCACAG AGGCTCCTGC ATCAGACCCC 
TTTCAGGAAC AGCCCACATC TACTGGAGCT TCTGGGGAAA CAGAGGAGGA AGAGAAAAAG 
GACCGTCTCA CTGAGGTTCC GGAGCGATCG GCCATTTTAC GGACCACCAT ACGATCACTG 
TCCCCTTTTC GAAGACACAG TTGGGGGCCG GGCAAAAATT TGGCAGGGGA GACAGAGATG 
AGTCAGCGCA GTTACAGTCT GGAAGGACTT GCACCAGAGA AGGAGGGAAG GAAAAGTCTG 
GTTCAGCAGC GTGTCGGAGA GTCCAGCCAG GAGCCCAAAT GTCCATCAAA GGTGGAAAAA 
GAAGACAGAG GCTCGCTTGT GTCTCTGACT GAAGAAGAGC AGGAGTCAGA TCTAGGCGAC 
TGCAGCAGTT TGGGCAGTCA GAAGTCCATC CAGTCGGGTC GGAGATGTGT CCCACATTCA 
CAACCCATCC TCACCAAATC TGTGTCCATG GTGGCCATCA GTCAGAAGGA CCTGGACGCA 
ATGGGACGGC TGCGACCCAA GCGACGAATC TCCTTCTCCT TCAGCATCTC GCCGATGCTC 
CCCAAATCTA AAACTCTCTT TTCTCTTGGC TCTTCATCCA GTGATGAGGA GGAGGCAGTC 
AGATTGAGTT CATTCACTGG AACATCAGGA TCATTAGAGT ACAGTATATC AGAGGAGGAT 
CCTGGGCCAT TACGGAGTGA CAGTGAAGGC AAAATTGGAG GAACCAAAGT GAGCAGGACA 
TTCAGTTACC TCAAGAGCAA GATGAGCAAG AAGAACAAGG AGAAGGAGAA AGAGAAGAAT 
AAGGAGCGTG AGCGAGATGC CAAAGACAAG GAGAGAAGAG CCTCAAACGG ACATCTCTTC 
ACTACGGTCA TGACTGTACC CACAATCCCC TGCCACCAGT GCAACAAACT CATCAACACT 
AAAGACTCTA TCCTCTGCAC CAATTGCAAT GCACAAGTCC ACAAAGGCTG CAAAGAGAGT 
CTTCCTGTCT GCGCTAAAGT CAAGATGAAG CAGCAAAAAC AGCAGCAGAC TGTTACAGAT 
TCTGCCTCGA CGCCTAGTGT CACCCTCAGA AGTAAAACTC TTCCAGCAAG GGATCGTCCG 
TGGTCCACAA TATCCATTCC CGATGACCAG CCGGCTCCCC TCGCTCCTCC TCGGAAAAGC 
CCCAGCAGCA TCATGTCTTT CAACAGCAAC CCACTGTCCA AGAGCATGTC CATTAATAAC 
ATAGCAGGAC AAATGGACGA CACTCCTCTT AAGAAGTTTC TGTCCCAGTC AACGGACTCT 
CTTCACAAGA CCAGTAAAGT CACGGAGTCT ACAGAATCTC TTACTGATGA GGGTACAGAG 
ATGATGGACA GCCAGCTGAT GGGAGAATTT GAAGCTGACG CCAAAGAGCT GGAGGCTGAT 
TCTTATAGCT TCACGGTAGA CAAGAAATTT CTTAAGCAGC TTAAAAAGGA TGTTATCAAA 
AGGCAAGACG TCATCTATGA GCTCATCCAG ACAGAAATGC ACCATGTAAG GACCTTAAAG 
ATCATGGCTG ATGTTTACAG CAAAGGACTA CTGAGAGAAG TTCAACTAGA AGCTCAGACG 
GTGGAGAAGA TGTTCCCCAT GTTGGACGAT GTTCTGGATT TGCACACACA GTTCTTTTCA 
CACCTGCTTG AAAGAAAGAA GGAATCAGGG AGCCAAGAAG AGGGCGGCTT TGTCATCCAA 
AGGATTGGCG ATGTGCTGGT TTCTCAGTTC TCAGGTTCCA GTGCTGAGAG AATGAAGAAG 
GTCTATGGGA AGTTCTGCGG CAGGCACAAT GAAGCTGTGA ACTTCTACAA GGAGCTTCAC 
ACCAAAGACA AACGCTTTCA AGCTTTCATC AAGAAAAAGA TGAGCAGCCC TGTTGTCCGA 
CGGCTAAGCA TTCCTGAGTG TATATTGTTA GTCACACAAC GAATCACAAA ATATCCCGTG 
TTACTACAGA GAATACTGCA GCACACGAAA GAAAACGAGG AGGACCATGC AGACGTTAGT 
CAAGGTCTGA AGCTGGTGAA GGAGGTGATT GCTGCCGTGG ACAATAAAGT GAATGAGCAT 
GAGAAGAAGA AGAGGCTAAA GGAAGTGTAC AGCCGGACGG ACAACAAGTC CATCATGAGG 
ATGAAGAGCG GGCAGATGTT TGCCAGAGAG GATCTGTTAC GTGGGCGGAA GTTAATTCAC 
GACGGGCCGC TGCAGCTCAA GAACTCAGCT GGCAGACTCA AGGATGTCCA GGCTCTGCTG 
CTGAGGGACG TGATTGTGTT CCTGCAGGAG AAAGATCAGA AGTACATTTT CGCCTCTTTG 
GACCAGCGCT CCACCGTCAT CTCCCTGCAG AAGCTCATTG TGAGGGAAGT AGCCAATGAG 
GAGAAAGCTC TGTTTCTGAT CACAGCAGGC ATCGAGAAGC CCGAGATGGT AGAAGTGTAC 
ACAAGCTCCA AAGAGGAGAG AAACACATGG ATGCAGCTCA TTCAGGAGGC CATGCAGTCT 
ATGGAGAGAG ATGATGATGA AGGTGTGCCA AGTGAAAACG AAGAGGACAG GAGACTTCAG 
GAAATCAAGG TCAAGGAGTT CAGAGATCAG CTGCACAAAA GAGACGAGCA GATCTTGACG 
TTACTGGAGG AGAAGGTGAA GTTGTTCAGG GACATGTGTG ACTGTGGTGC ACCGGACGAG 
ACGGGGCTTC GTAATCGAAT GCTCTTCAGG GCCACGACGG ATGATGTCAC CAGAGGAGAG 
CCAATCATGA AGGAGGCTCT GAAGGAAGTT GAAACGCTGC AGGTTCTGGT TAATGACAGT 
CTTGGCGGTG CGGTTCAGGA AGGTGTGTGC GCGGCTGGAC ATGTGACTTT GCCACGACGA 
GCGGAAACAT TTGGAGGTTT CGACAGTCAC CAGATGAACA TCTCTAAGAG CAAAGATGGA 
GACAGAGAGG AGCTGGAGGA GTCTGCAGAG CTCCGCAGGA CTGAGTCAGA CAGTGTGTTG 
AAGAAGACGA GTAAGACCAA CCCTTTACTG CTGGTCAGGA GAAACAATGA GCAGGTTCTT 
CAGAGCGTTG CTCATCTTCA TGACCTGCTG AACACATTAC AAGCTGTGGT GGTCCAGCAG 
GACACCTTCA TCGAAGACCA GCGTCACGCT CATCCCTCCT CACGCCACCC ATCCATCTCC 
TCCTCTACCT CCTCTTTATC ATCCACCTCA TCCCGGCCGA GCTCTCTAAT CGAGCAGGAG 
AAACAGCGCA GCCTGGAGAA GCAGCGGCAG GAGGTGGCCA GCCTCCAAAA ACAGCAGGCG 
GCCCACGCCG AGGAGAGGCG GCGCAGGGAA AAGCAGTGGG AGACCCGAGA GAAAGAGCTA 
ACCCAGAGGG AGATGCAGAT CCAGAGCCAA GAAGAGGAGG CGCAGAGGAG AGCCAGACAG 
CTGGAGGAGG AGAAACAGGA GCTGCAGAGT AAGAAAGAGG AGTATCAGAG AGACCTGGCA 
CGACTGCGGG ACAGCCAGAA GAGACTGGAG AGAGAGAGAG AGCAGGTGCA GAGAGAGGCA 
GATGAGCTTA AACTGACTGA GGAAGTGACA AAGAACCGGT CTCCGTCAAC CACGTCTGAA 
GACTCCACGA AGTTCCAGAG CACCGGTTCA TTAGACCGTG ACCCATCAGA GATGGAGCTC 
TCCTCCTCCC CGTCAGCCAG AGACTTCCTG TCCCGCATGG ACTCCAAACG CAAAGGGAAA 
AACCTCAATC CATTCTCATC CAACTCAAGC CAGAAGAGCC AGAACAGCGA GGCTCAGAGC 
CAGATGCCCA GCCGTCTGCT CCAGCTGGCC AAACCCAAGG AGAAGAAGGA CAAGAAGAAG 
AAGAAGGGGA AAGGCCAGCA GAGCCAGACT GCAGACTCTC AGAGCACTGT TGTTCCAGGC 
GCTACCCCAG ACGGAGAGAT CTTTTTCTGC TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_021326316.1
CDS	398..7930
Translation	MKLNPQQAPLYGECLLTVQLCEDECVEDEDDVEFYLLFAGSTQRHVSSTLKISHVTLQAVCPAHDRSETVRVTLCQARPGGSVDPVSEECFQFVQDLALDMAQFLVNAAGKTEGALLLDECQIPLKECERLDDTLALALRHLPLPAGWSVLGTDLDTGTCTGSGIAEPGPHDTLLHFAAKRGLRKVALFLLQQPGGREALRLPNKQGRTPAKVAQKRGHSQLHKLLTEVDNLPELETKTSKWCYPEGRVLQHHPKLNTYTLTLEDVPRSSPPSLQCDVEELQRLIQSHQKEDPPTPHKSALLILNRDSSNSTETDDCLPPNSVEPEHQEHNKQEETCKSLLNSLTFEDGLCVHQNGTGDHVQLLDGDVEPDLPAVGDTGNALDKTDCLQRRVDGDSVAFSAPSCGNQREEADIELCIISAKQGVASQPDKGKQEEQEACQKRSETEKDYNTESGNLGLQDMGHAQSQGLLPTETGQPTQREVESEGSTDVCWRGDLPEEREGDMSSNQEIDSYSSALSSHNLEGNSQHDGETEPQLPTQCSEGTDEAKLTLIQVNSEVTLSIRSEDTVISDSVDKPHGLLETETGNLSEHTKGNTESAADLVADDASVKQNSGFSVLEGDLLESLSLRPNMAPGPVHKVQQGLSPVPETSSHVGPALDPFSETTESFQQPNQSTQLLETALECEDVSLNNSTGDINAIAGVSVDISAEVSLCQNEGCPPDGSKEEVAPSERIPDLKELTEEPESVEDIIVSDVSDLDDKSQPFEESSLPIDSECFIRGDFQNDESTVQSSECTGGDINVGTLLGEEEATEKIEQPDSSLKLVELIPEEYTGDATNENKKIEPEEQSILLPTLQGESGFKDVATEEPVQEREIDSTSEETISDLPDPSEITNNDCAESNAHCQLSESSKESANTSSEPAECVNDLDLSCALPQTEIEGSRPSEGPLIDGVTICAEIPIDDKGVDMATNMSKTTISEDCLSSPMDTTSCSVLESSSMSDPLNASSSTAELTSPLDINSGLHQDLEHTSPVDIDNGLPEEPPPSDTPDCLNGLHQDSPDSLMEHSGTPQPCSEEQCVDQITDDSTSAEDAADGHQDSSVDIANSEAIHRETETFYSVDASQDSVLQEKKSSSVSSEIPSLTPSPESIRHKESPSTMRGSASDGDSLFSQDLGEDSVFNNKAEDSITITSGVSMSYSSSTDDSSSLGTTCATPQTGTEAPASDPFQEQPTSTGASGETEEEEKKDRLTEVPERSAILRTTIRSLSPFRRHSWGPGKNLAGETEMSQRSYSLEGLAPEKEGRKSLVQQRVGESSQEPKCPSKVEKEDRGSLVSLTEEEQESDLGDCSSLGSQKSIQSGRRCVPHSQPILTKSVSMVAISQKDLDAMGRLRPKRRISFSFSISPMLPKSKTLFSLGSSSSDEEEAVRLSSFTGTSGSLEYSISEEDPGPLRSDSEGKIGGTKVSRTFSYLKSKMSKKNKEKEKEKNKERERDAKDKERRASNGHLFTTVMTVPTIPCHQCNKLINTKDSILCTNCNAQVHKGCKESLPVCAKVKMKQQKQQQTVTDSASTPSVTLRSKTLPARDRPWSTISIPDDQPAPLAPPRKSPSSIMSFNSNPLSKSMSINNIAGQMDDTPLKKFLSQSTDSLHKTSKVTESTESLTDEGTEMMDSQLMGEFEADAKELEADSYSFTVDKKFLKQLKKDVIKRQDVIYELIQTEMHHVRTLKIMADVYSKGLLREVQLEAQTVEKMFPMLDDVLDLHTQFFSHLLERKKESGSQEEGGFVIQRIGDVLVSQFSGSSAERMKKVYGKFCGRHNEAVNFYKELHTKDKRFQAFIKKKMSSPVVRRLSIPECILLVTQRITKYPVLLQRILQHTKENEEDHADVSQGLKLVKEVIAAVDNKVNEHEKKKRLKEVYSRTDNKSIMRMKSGQMFAREDLLRGRKLIHDGPLQLKNSAGRLKDVQALLLRDVIVFLQEKDQKYIFASLDQRSTVISLQKLIVREVANEEKALFLITAGIEKPEMVEVYTSSKEERNTWMQLIQEAMQSMERDDDEGVPSENEEDRRLQEIKVKEFRDQLHKRDEQILTLLEEKVKLFRDMCDCGAPDETGLRNRMLFRATTDDVTRGEPIMKEALKEVETLQVLVNDSLGGAVQEGVCAAGHVTLPRRAETFGGFDSHQMNISKSKDGDREELEESAELRRTESDSVLKKTSKTNPLLLVRRNNEQVLQSVAHLHDLLNTLQAVVVQQDTFIEDQRHAHPSSRHPSISSSTSSLSSTSSRPSSLIEQEKQRSLEKQRQEVASLQKQQAAHAEERRRREKQWETREKELTQREMQIQSQEEEAQRRARQLEEEKQELQSKKEEYQRDLARLRDSQKRLEREREQVQREADELKLTEEVTKNRSPSTTSEDSTKFQSTGSLDRDPSEMELSSSPSARDFLSRMDSKRKGKNLNPFSSNSSQKSQNSEAQSQMPSRLLQLAKPKEKKDKKKKKGKGQQSQTADSQSTVVPGATPDGEIFFC

Target ORF information:

RefSeq Version	XM_021470641.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio wu:fi15d04 (wu:fi15d04), transcript variant X13, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_021470641.1

ORF Insert Sequence:

ATGAAACTGA ATCCACAGCA AGCCCCATTA TATGGAGAGT GTCTGCTGAC GGTCCAGCTC 
TGTGAGGATG AGTGTGTTGA GGATGAGGAT GATGTGGAGT TTTACCTGCT CTTTGCTGGA 
TCCACACAGA GACATGTGTC CAGCACACTG AAGATCAGCC ATGTCACGCT GCAGGCCGTC 
TGTCCAGCTC ATGATCGCAG TGAAACGGTA CGGGTCACAC TATGCCAGGC TCGTCCCGGC 
GGCTCAGTGG ACCCAGTGTC CGAGGAGTGT TTCCAGTTTG TCCAGGACCT GGCTCTGGAC 
ATGGCCCAGT TCCTGGTAAA TGCGGCGGGG AAAACAGAAG GAGCGCTGCT GCTGGACGAG 
TGTCAGATCC CACTGAAAGA GTGCGAGCGG CTGGACGATA CTCTTGCTCT GGCTCTCCGG 
CATCTGCCTT TGCCTGCTGG ATGGAGCGTT TTGGGCACAG ATTTGGACAC GGGCACTTGC 
ACAGGCTCGG GCATCGCAGA GCCTGGACCA CACGATACAC TTTTGCACTT CGCTGCGAAG 
CGCGGACTAC GCAAGGTTGC ACTCTTTCTG CTCCAGCAAC CAGGGGGAAG AGAGGCTCTC 
CGGCTTCCCA ACAAACAGGG TCGCACACCG GCCAAGGTCG CCCAGAAGAG GGGACACTCT 
CAGCTCCACA AACTGCTCAC GGAGGTTGAC AATCTGCCCG AGCTGGAGAC GAAAACATCT 
AAGTGGTGTT ACCCAGAAGG CAGAGTCCTG CAACACCACC CCAAGCTAAA CACTTACACC 
CTTACCCTGG AGGATGTGCC CAGGAGCTCC CCACCAAGCC TTCAGTGTGA TGTGGAGGAG 
CTCCAGCGTC TCATTCAGTC ACATCAGAAG GAAGATCCTC CAACACCGCA TAAGTCAGCA 
CTGCTGATTC TCAATAGAGA CTCCAGTAAT AGCACAGAGA CAGATGACTG CCTTCCGCCC 
AACTCAGTGG AACCTGAGCA TCAGGAGCAC AACAAACAAG AAGAAACTTG CAAATCGCTT 
TTAAACAGCC TTACCTTTGA GGATGGATTG TGTGTTCATC AAAACGGCAC AGGAGATCAT 
GTCCAGTTGC TGGATGGGGA CGTGGAGCCT GACCTGCCAG CTGTAGGAGA TACAGGGAAT 
GCATTAGATA AGACTGACTG TTTGCAGCGG CGGGTAGATG GTGATTCTGT GGCTTTCAGT 
GCCCCTTCCT GTGGAAACCA GCGGGAGGAA GCCGATATAG AGCTCTGCAT AATCAGTGCC 
AAGCAGGGGG TTGCTTCACA GCCTGATAAA GGGAAGCAAG AAGAGCAAGA GGCTTGCCAG 
AAGAGGTCAG AGACTGAAAA GGACTATAAC ACTGAATCTG GCAACCTTGG GCTTCAAGAC 
ATGGGCCACG CACAATCTCA GGGTCTCCTC CCCACGGAAA CTGGTCAGCC TACACAAAGG 
GAGGTGGAGA GTGAGGGGAG TACAGATGTC TGTTGGCGTG GGGACTTACC AGAGGAAAGG 
GAAGGGGACA TGAGTAGCAA CCAGGAAATA GACTCATATT CTTCAGCACT TTCTTCCCAT 
AATCTAGAGG GAAACAGCCA GCACGATGGT GAGACTGAGC CCCAGTTGCC TACACAATGC 
TCAGAGGGAA CAGATGAAGC TAAATTGACT TTAATTCAGG TAAATTCTGA AGTTACTTTG 
TCTATACGAA GCGAGGATAC CGTTATTTCT GACAGTGTTG ACAAGCCACA TGGTCTCTTA 
GAGACGGAGA CAGGAAATCT CAGCGAACAC ACCAAAGGCA ACACTGAGTC AGCTGCTGAT 
TTGGTTGCGG ATGACGCTAG TGTAAAGCAG AACTCTGGAT TTAGCGTTTT AGAGGGGGAT 
CTTTTGGAAT CATTAAGTCT ACGGCCAAAC ATGGCACCGG GACCAGTGCA TAAGGTTCAG 
CAAGGTCTGT CACCAGTTCC TGAGACTTCC TCTCATGTAG GTCCAGCTTT GGACCCCTTT 
TCTGAGACTA CAGAAAGTTT TCAACAGCCG AATCAATCCA CACAGCTTTT AGAAACTGCA 
TTAGAATGTG AGGATGTTTC ATTAAATAAC TCAACCGGTG ACATTAATGC AATAGCAGGG 
GTTTCTGTTG ACATTTCTGC AGAGGTTTCA CTTTGCCAGA ATGAAGGGTG TCCTCCTGAT 
GGAAGCAAAG AAGAGGTAGC CCCCAGTGAG AGGATACCAG ACCTTAAAGA GCTTACGGAA 
GAGCCGGAAT CAGTTGAGGA CATTATTGTC TCAGATGTCT CAGATTTGGA TGACAAATCT 
CAACCTTTTG AAGAGTCTTC TTTGCCTATC GACTCTGAAT GTTTTATAAG AGGTGATTTC 
CAAAACGATG AGTCTACAGT ACAGTCTTCT GAATGTACTG GAGGTGACAT AAATGTAGGA 
ACACTCTTAG GAGAAGAAGA GGCAACTGAA AAAATAGAAC AGCCAGACTC TTCACTTAAA 
CTGGTTGAGC TAATTCCTGA GGAATATACT GGAGATGCCA CAAACGAAAA TAAAAAAATT 
GAGCCAGAGG AACAATCTAT TTTACTGCCA ACTTTGCAAG GGGAATCAGG ATTTAAGGAT 
GTTGCCACAG AGGAACCTGT ACAGGAAAGG GAAATTGACT CCACTTCAGA GGAAACAATC 
AGCGATCTCC CTGATCCATC GGAAATCACA AACAATGATT GTGCTGAATC CAATGCACAC 
TGCCAGCTCT CAGAATCCTC CAAGGAGAGC GCAAACACAT CCTCAGAACC TGCTGAATGT 
GTGAATGACC TAGACTTGTC TTGTGCTTTA CCTCAAACAG AAATCGAAGG CAGCAGACCT 
TCTGAGGGCC CCTTAATTGA TGGAGTAACA ATATGTGCTG AAATTCCCAT TGATGACAAA 
GGTGTTGACA TGGCTACCAA TATGAGTAAG ACGACCATCT CTGAGGATTG TCTGTCCTCT 
CCTATGGACA CCACCAGCTG TTCAGTTCTG GAGAGCTCCT CCATGTCTGA CCCACTCAAT 
GCCTCATCCT CAACAGCTGA GCTCACTTCC CCTTTGGATA TTAATAGTGG ATTGCATCAG 
GATTTGGAAC ACACTTCCCC AGTGGATATA GACAATGGTT TGCCTGAGGA ACCGCCGCCG 
TCAGACACAC CTGATTGCCT GAATGGATTA CATCAAGATT CTCCTGATAG TTTGATGGAG 
CACAGTGGAA CTCCTCAGCC ATGTTCTGAG GAACAGTGTG TGGATCAAAT CACAGATGAC 
AGCACTTCAG CAGAGGATGC AGCGGACGGA CACCAAGACT CCTCAGTAGA CATAGCAAAT 
TCCGAAGCGA TCCACAGAGA GACCGAAACA TTTTATTCAG TTGATGCCAG TCAAGACTCT 
GTACTCCAGG AGAAGAAATC TTCTTCAGTG AGCTCAGAAA TCCCCTCTTT GACCCCCTCA 
CCTGAGAGCA TACGTCACAA AGAGTCGCCA AGCACTATGC GAGGGTCCGC CAGCGATGGA 
GACTCTCTGT TTAGTCAGGA TCTGGGCGAG GACAGCGTTT TTAATAATAA GGCTGAGGAC 
AGCATTACCA TCACCAGTGG CGTGTCCATG TCCTATTCGT CCTCCACAGA TGACTCTTCC 
AGCTTGGGAA CCACTTGCGC CACCCCTCAG ACTGGCACAG AGGCTCCTGC ATCAGACCCC 
TTTCAGGAAC AGCCCACATC TACTGGAGCT TCTGGGGAAA CAGAGGAGGA AGAGAAAAAG 
GACCGTCTCA CTGAGGTTCC GGAGCGATCG GCCATTTTAC GGACCACCAT ACGATCACTG 
TCCCCTTTTC GAAGACACAG TTGGGGGCCG GGCAAAAATT TGGCAGGGGA GACAGAGATG 
AGTCAGCGCA GTTACAGTCT GGAAGGACTT GCACCAGAGA AGGAGGGAAG GAAAAGTCTG 
GTTCAGCAGC GTGTCGGAGA GTCCAGCCAG GAGCCCAAAT GTCCATCAAA GGTGGAAAAA 
GAAGACAGAG GCTCGCTTGT GTCTCTGACT GAAGAAGAGC AGGAGTCAGA TCTAGGCGAC 
TGCAGCAGTT TGGGCAGTCA GAAGTCCATC CAGTCGGGTC GGAGATGTGT CCCACATTCA 
CAACCCATCC TCACCAAATC TGTGTCCATG GTGGCCATCA GTCAGAAGGA CCTGGACGCA 
ATGGGACGGC TGCGACCCAA GCGACGAATC TCCTTCTCCT TCAGCATCTC GCCGATGCTC 
CCCAAATCTA AAACTCTCTT TTCTCTTGGC TCTTCATCCA GTGATGAGGA GGAGGCAGTC 
AGATTGAGTT CATTCACTGG AACATCAGGA TCATTAGAGT ACAGTATATC AGAGGAGGAT 
CCTGGGCCAT TACGGAGTGA CAGTGAAGGC AAAATTGGAG GAACCAAAGT GAGCAGGACA 
TTCAGTTACC TCAAGAGCAA GATGAGCAAG AAGAACAAGG AGAAGGAGAA AGAGAAGAAT 
AAGGAGCGTG AGCGAGATGC CAAAGACAAG GAGAGAAGAG CCTCAAACGG ACATCTCTTC 
ACTACGGTCA TGACTGTACC CACAATCCCC TGCCACCAGT GCAACAAACT CATCAACACT 
AAAGACTCTA TCCTCTGCAC CAATTGCAAT GCACAAGTCC ACAAAGGCTG CAAAGAGAGT 
CTTCCTGTCT GCGCTAAAGT CAAGATGAAG CAGCAAAAAC AGCAGCAGAC TGTTACAGAT 
TCTGCCTCGA CGCCTAGTGT CACCCTCAGA AGTAAAACTC TTCCAGCAAG GGATCGTCCG 
TGGTCCACAA TATCCATTCC CGATGACCAG CCGGCTCCCC TCGCTCCTCC TCGGAAAAGC 
CCCAGCAGCA TCATGTCTTT CAACAGCAAC CCACTGTCCA AGAGCATGTC CATTAATAAC 
ATAGCAGGAC AAATGGACGA CACTCCTCTT AAGAAGTTTC TGTCCCAGTC AACGGACTCT 
CTTCACAAGA CCAGTAAAGT CACGGAGTCT ACAGAATCTC TTACTGATGA GGGTACAGAG 
ATGATGGACA GCCAGCTGAT GGGAGAATTT GAAGCTGACG CCAAAGAGCT GGAGGCTGAT 
TCTTATAGCT TCACGGTAGA CAAGAAATTT CTTAAGCAGC TTAAAAAGGA TGTTATCAAA 
AGGCAAGACG TCATCTATGA GCTCATCCAG ACAGAAATGC ACCATGTAAG GACCTTAAAG 
ATCATGGCTG ATGTTTACAG CAAAGGACTA CTGAGAGAAG TTCAACTAGA AGCTCAGACG 
GTGGAGAAGA TGTTCCCCAT GTTGGACGAT GTTCTGGATT TGCACACACA GTTCTTTTCA 
CACCTGCTTG AAAGAAAGAA GGAATCAGGG AGCCAAGAAG AGGGCGGCTT TGTCATCCAA 
AGGATTGGCG ATGTGCTGGT TTCTCAGTTC TCAGGTTCCA GTGCTGAGAG AATGAAGAAG 
GTCTATGGGA AGTTCTGCGG CAGGCACAAT GAAGCTGTGA ACTTCTACAA GGAGCTTCAC 
ACCAAAGACA AACGCTTTCA AGCTTTCATC AAGAAAAAGA TGAGCAGCCC TGTTGTCCGA 
CGGCTAAGCA TTCCTGAGTG TATATTGTTA GTCACACAAC GAATCACAAA ATATCCCGTG 
TTACTACAGA GAATACTGCA GCACACGAAA GAAAACGAGG AGGACCATGC AGACGTTAGT 
CAAGGTCTGA AGCTGGTGAA GGAGGTGATT GCTGCCGTGG ACAATAAAGT GAATGAGCAT 
GAGAAGAAGA AGAGGCTAAA GGAAGTGTAC AGCCGGACGG ACAACAAGTC CATCATGAGG 
ATGAAGAGCG GGCAGATGTT TGCCAGAGAG GATCTGTTAC GTGGGCGGAA GTTAATTCAC 
GACGGGCCGC TGCAGCTCAA GAACTCAGCT GGCAGACTCA AGGATGTCCA GGCTCTGCTG 
CTGAGGGACG TGATTGTGTT CCTGCAGGAG AAAGATCAGA AGTACATTTT CGCCTCTTTG 
GACCAGCGCT CCACCGTCAT CTCCCTGCAG AAGCTCATTG TGAGGGAAGT AGCCAATGAG 
GAGAAAGCTC TGTTTCTGAT CACAGCAGGC ATCGAGAAGC CCGAGATGGT AGAAGTGTAC 
ACAAGCTCCA AAGAGGAGAG AAACACATGG ATGCAGCTCA TTCAGGAGGC CATGCAGTCT 
ATGGAGAGAG ATGATGATGA AGGTGTGCCA AGTGAAAACG AAGAGGACAG GAGACTTCAG 
GAAATCAAGG TCAAGGAGTT CAGAGATCAG CTGCACAAAA GAGACGAGCA GATCTTGACG 
TTACTGGAGG AGAAGGTGAA GTTGTTCAGG GACATGTGTG ACTGTGGTGC ACCGGACGAG 
ACGGGGCTTC GTAATCGAAT GCTCTTCAGG GCCACGACGG ATGATGTCAC CAGAGGAGAG 
CCAATCATGA AGGAGGCTCT GAAGGAAGTT GAAACGCTGC AGGTTCTGGT TAATGACAGT 
CTTGGCGGTG CGGTTCAGGA AGGTGTGTGC GCGGCTGGAC ATGTGACTTT GCCACGACGA 
GCGGAAACAT TTGGAGGTTT CGACAGTCAC CAGATGAACA TCTCTAAGAG CAAAGATGGA 
GACAGAGAGG AGCTGGAGGA GTCTGCAGAG CTCCGCAGGA CTGAGTCAGA CAGTGTGTTG 
AAGAAGACGA GTAAGACCAA CCCTTTACTG CTGGTCAGGA GAAACAATGA GCAGGTTCTT 
CAGAGCGTTG CTCATCTTCA TGACCTGCTG AACACATTAC AAGCTGTGGT GGTCCAGCAG 
GACACCTTCA TCGAAGACCA GCGTCACGCT CATCCCTCCT CACGCCACCC ATCCATCTCC 
TCCTCTACCT CCTCTTTATC ATCCACCTCA TCCCGGCCGA GCTCTCTAAT CGAGCAGGAG 
AAACAGCGCA GCCTGGAGAA GCAGCGGCAG GAGGTGGCCA GCCTCCAAAA ACAGCAGGCG 
GCCCACGCCG AGGAGAGGCG GCGCAGGGAA AAGCAGTGGG AGACCCGAGA GAAAGAGCTA 
ACCCAGAGGG AGATGCAGAT CCAGAGCCAA GAAGAGGAGG CGCAGAGGAG AGCCAGACAG 
CTGGAGGAGG AGAAACAGGA GCTGCAGAGT AAGAAAGAGG AGTATCAGAG AGACCTGGCA 
CGACTGCGGG ACAGCCAGAA GAGACTGGAG AGAGAGAGAG AGCAGGTGCA GAGAGAGGCA 
GATGAGCTTA AACTGACTGA GGAAGTGACA AAGAACCGGT CTCCGTCAAC CACGTCTGAA 
GACTCCACGA AGTTCCAGAG CACCGGTTCA TTAGACCGTG ACCCATCAGA GATGGAGCTC 
TCCTCCTCCC CGTCAGCCAG AGACTTCCTG TCCCGCATGG ACTCCAAACG CAAAGGGAAA 
AACCTCAATC CATTCTCATC CAACTCAAGC CAGAAGAGCC AGAACAGCGA GGCTCAGAGC 
CAGATGCCCA GCCGTCTGCT CCAGCTGGCC AAACCCAAGG AGAAGAAGGA CAAGAAGAAG 
AAGAAGGGGA AAGGCCAGCA GAGCCAGACT GCAGACTCTC AGAGCACTGT TGTTCCAGGC 
GCTACCCCAG ACGGAGAGAT CTTTTTCTGC TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

wu:fi15d04 ( XM_009297833.2 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_009296108.1 Danio rerio wu:fi15d04 (wu:fi15d04), transcript variant X1, mRNA.

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CloneID	ODa39927
Clone ID Related Accession (Same CDS sequence)	XM_009297833.2
Accession Version	XM_009297833.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7692bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-23
Organism	Danio rerio(Zebrafish)
Product	A-kinase anchor protein 13 isoform X1
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007136.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_009297833.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAGGGTG TTCCACAAGT GGCCAGTGCC ATGAAACTGA ATCCACAGCA AGCCCCATTA 
TATGGAGAGT GTCTGCTGAC GGTCCAGCTC TGTGAGGATG AGTGTGTTGA GGATGAGGAT 
GATGTGGAGT TTTACCTGCT CTTTGCTGGA TCCACACAGA GACATGTGTC CAGCACACTG 
AAGATCAGCC ATGTCACGCT GCAGGCCGTC TGTCCAGCTC ATGATCGCAG TGAAACGGTA 
CGGGTCACAC TATGCCAGGC TCGTCCCGGC GGCTCAGTGG ACCCAGTGTC CGAGGAGTGT 
TTCCAGTTTG TCCAGGACCT GGCTCTGGAC ATGGCCCAGT TCCTGGTAAA TGCGGCGGGG 
AAAACAGAAG GAGCGCTGCT GCTGGACGAG TGTCAGATCC CACTGAAAGA GTGCGAGCGG 
CTGGACGATA CTCTTGCTCT GGCTCTCCGG CATCTGCCTT TGCCTGCTGG ATGGAGCGTT 
TTGGGCACAG ATTTGGACAC GGGCACTTGC ACAGGCTCGG GCATCGCAGA GCCTGGACCA 
CACGATACAC TTTTGCACTT CGCTGCGAAG CGCGGACTAC GCAAGGTTGC ACTCTTTCTG 
CTCCAGCAAC CAGGGGGAAG AGAGGCTCTC CGGCTTCCCA ACAAACAGGG TCGCACACCG 
GCCAAGGTCG CCCAGAAGAG GGGACACTCT CAGCTCCACA AACTGCTCAC GGAGGTTGAC 
AATCTGCCCG AGCTGGAGAC GAAAACATCT AAGTGGTGTT ACCCAGAAGG CAGAGTCCTG 
CAACACCACC CCAAGCTAAA CACTTACACC CTTACCCTGG AGGATGTGCC CAGGAGCTCC 
CCACCAAGCC TTCAGTGTGA TGTGGAGGAG CTCCAGCGTC TCATTCAGTC ACATCAGAAG 
GAAGATCCTC CAACACCGCA TAAGTCAGCA CTGCTGATTC TCAATAGAGA CTCCAGTAAT 
AGCACAGAGA CAGATGACTG CCTTCCGCCC AACTCAGTGG AACCTGAGCA TCAGGAGCAC 
AACAAACAAG AAGAAACTTG CAAATCGCTT TTAAACAGCC TTACCTTTGA GGATGGATTG 
TGTGTTCATC AAAACGGCAC AGGAGATCAT GTCCAGTTGC TGGATGGGGA CGTGGAGCCT 
GACCTGCCAG CTGTAGGAGA TACAGGGAAT GCATTAGATA AGACTGACTG TTTGCAGCGG 
CGGGTAGATG GTGATTCTGT GGCTTTCAGT GCCCCTTCCT GTGGAAACCA GCGGGAGGAA 
GCCGATATAG AGCTCTGCAT AATCAGTGCC AAGCAGGGGG TTGCTTCACA GCCTGATAAA 
GGGAAGCAAG AAGAGCAAGA GGCTTGCCAG AAGAGGTCAG AGACTGAAAA GGACTATAAC 
ACTGAATCTG GCAACCTTGG GCTTCAAGAC ATGGGCCACG CACAATCTCA GGGTCTCCTC 
CCCACGGAAA CTGGTCAGCC TACACAAAGG GAGGTGGAGA GTGAGGGGAG TACAGATGTC 
TGTTGGCGTG GGGACTTACC AGAGGAAAGG GAAGGGGACA TGAGTAGCAA CCAGGAAATA 
GACTCATATT CTTCAGCACT TTCTTCCCAT AATCTAGAGG GAAACAGCCA GCACGATGGT 
GAGACTGAGC CCCAGTTGCC TACACAATGC TCAGAGGGAA CAGATGAAGC TAAATTGACT 
TTAATTCAGG TAAATTCTGA AGTTACTTTG TCTATACGAA GCGAGGATAC CGTTATTTCT 
GACAGTGTTG ACAAGCCACA TGGTCTCTTA GAGACGGAGA CAGGAAATCT CAGCGAACAC 
ACCAAAGGCA ACACTGAGTC AGCTGCTGAT TTGGTTGCGG ATGACGCTAG TGTAAAGCAG 
AACTCTGGAT TTAGCGTTTT AGAGGGGGAT CTTTTGGAAT CATTAAGTCT ACGGCCAAAC 
ATGGCACCGG GACCAGTGCA TAAGGTTCAG CAAGGTCTGT CACCAGTTCC TGAGACTTCC 
TCTCATGTAG GTCCAGCTTT GGACCCCTTT TCTGAGACTA CAGAAAGTTT TCAACAGCCG 
AATCAATCCA CACAGCTTTT AGAAACTGCA TTAGAATGTG AGGATGTTTC ATTAAATAAC 
TCAACCGGTG ACATTAATGC AATAGCAGGG GTTTCTGTTG ACATTTCTGC AGAGGTTTCA 
CTTTGCCAGA ATGAAGGGTG TCCTCCTGAT GGAAGCAAAG AAGAGGTAGC CCCCAGTGAG 
AGGATACCAG ACCTTAAAGA GCTTACGGAA GAGCCGGAAT CAGTTGAGGA CATTATTGTC 
TCAGATGTCT CAGATTTGGA TGACAAATCT CAACCTTTTG AAGAGTCTTC TTTGCCTATC 
GACTCTGAAT GTTTTATAAG AGGTGATTTC CAAAACGATG AGTCTACAGT ACAGTCTTCT 
GAATGTACTG GAGGTGACAT AAATGTAGGA ACACTCTTAG GAGAAGAAGA GGCAACTGAA 
AAAATAGAAC AGCCAGACTC TTCACTTAAA CTGGTTGAGC TAATTCCTGA GGAATATACT 
GGAGATGCCA CAAACGAAAA TAAAAAAATT GAGCCAGAGG AACAATCTAT TTTACTGCCA 
ACTTTGCAAG GGGAATCAGG ATTTAAGGAT GTTGCCACAG AGGAACCTGT ACAGGAAAGG 
GAAATTGACT CCACTTCAGA GGAAACAATC AGCGATCTCC CTGATCCATC GGAAATCACA 
AACAATGATT GTGCTGAATC CAATGCACAC TGCCAGCTCT CAGAATCCTC CAAGGAGAGC 
GCAAACACAT CCTCAGAACC TGCTGAATGT GTGAATGACC TAGACTTGTC TTGTGCTTTA 
CCTCAAACAG AAATCGAAGG CAGCAGACCT TCTGAGGGCC CCTTAATTGA TGGAGTAACA 
ATATGTGCTG AAATTCCCAT TGATGACAAA GGTGTTGACA TGGCTACCAA TATGAGTAAG 
ACGACCATCT CTGAGGATTG TCTGTCCTCT CCTATGGACA CCACCAGCTG TTCAGTTCTG 
GAGAGCTCCT CCATGTCTGA CCCACTCAAT GCCTCATCCT CAACAGCTGA GCTCACTTCC 
CCTTTGGATA TTAATAGTGG ATTGCATCAG GATTTGGAAC ACACTTCCCC AGTGGATATA 
GACAATGGTT TGCCTGAGGA ACCGCCGCCG TCAGACACAC CTGATTGCCT GAATGGATTA 
CATCAAGATT CTCCTGATAG TTTGATGGAG CACAGTGGAA CTCCTCAGCC ATGTTCTGAG 
GAACAGTGTG TGGATCAAAT CACAGATGAC AGCACTTCAG CAGAGGATGC AGCGGACGGA 
CACCAAGACT CCTCAGTAGA CATAGCAAAT TCCGAAGCGA TCCACAGAGA GACCGAAACA 
TTTTATTCAG TTGATGCCAG TCAAGACTCT GTACTCCAGG AGAAGAAATC TTCTTCAGTG 
AGCTCAGAAA TCCCCTCTTT GACCCCCTCA CCTGAGAGCA TACGTCACAA AGAGTCGCCA 
AGCACTATGC GAGGGTCCGC CAGCGATGGA GACTCTCTGT TTAGTCAGGA TCTGGGCGAG 
GACAGCGTTT TTAATAATAA GGCTGAGGAC AGCATTACCA TCACCAGTGG CGTGTCCATG 
TCCTATTCGT CCTCCACAGA TGACTCTTCC AGCTTGGGAA CCACTTGCGC CACCCCTCAG 
ACTGGCACAG AGGCTCCTGC ATCAGACCCC TTTCAGGAAC AGCCCACATC TACTGGAGCT 
TCTGGGGAAA CAGAGGAGGA AGAGAAAAAG GACCGTCTCA CTGAGGTTCC GGAGCGATCG 
GCCATTTTAC GGACCACCAT ACGATCACTG TCCCCTTTTC GAAGACACAG TTGGGGGCCG 
GGCAAAAATT TGGCAGGGGA GACAGAGATG AGTCAGCGCA GTGCGGCACA AGGTGAAGTG 
GAGCGCAGAT CTGTTGGTCA TAGGAGAAGC ATGAGCTGGT GTCCTTCTGT TGTCCTGCGC 
TCTGAAAATG ATGAAATGAA TGAAAGAAGT TACAGTCTGG AAGGACTTGC ACCAGAGAAG 
GAGGGAAGGA AAAGTCTGGT TCAGCAGCGT GTCGGAGAGT CCAGCCAGGA GCCCAAATGT 
CCATCAAAGG TGGAAAAAGA AGACAGAGGC TCGCTTGTGT CTCTGACTGA AGAAGAGCAG 
GAGTCAGATC TAGGCGACTG CAGCAGTTTG GGCAGTCAGA AGTCCATCCA GTCGGGTCGG 
AGATGTGTCC CACATTCACA ACCCATCCTC ACCAAATCTG TGTCCATGGT GGCCATCAGT 
CAGAAGGACC TGGACGCAAT GGGACGGCTG CGACCCAAGC GACGAATCTC CTTCTCCTTC 
AGCATCTCGC CGATGCTCCC CAAATCTAAA ACTCTCTTTT CTCTTGGCTC TTCATCCAGT 
GATGAGGAGG AGGCAGTCAG ATTGAGTTCA TTCACTGGAA CATCAGGATC ATTAGAGTAC 
AGTATATCAG AGGAGGATCC TGGGCCATTA CGGAGTGACA GTGAAGGCAA AATTGGAGGA 
ACCAAAGTGA GCAGGACATT CAGTTACCTC AAGAGCAAGA TGAGCAAGAA GAACAAGCAG 
GAGAAGGAGA AAGAGAAGAA TAAGGAGCGT GAGCGAGATG CCAAAGACAA GGAGAGAAGA 
GCCTCAAACG GACATCTCTT CACTACGGTC ATGACTGTAC CCACAATCCC CTGCCACCAG 
TGCAACAAAC TCATCAACAC TAAAGACTCT ATCCTCTGCA CCAATTGCAA TGCACAAGTC 
CACAAAGGCT GCAAAGAGAG TCTTCCTGTC TGCGCTAAAG TCAAGATGAA GCAGCAAAAA 
CAGCAGCAGA CTGTTACAGA TTCTGCCTCG ACGCCTAGTG TCACCCTCAG AAGTAAAACT 
CTTCCAGCAA GGGATCGTCC GTGGTCCACA ATATCCATTC CCGATGACCA GCCGGCTCCC 
CTCGCTCCTC CTCGGAAAAG CCCCAGCAGC ATCATGTCTT TCAACAGCAA CCCACTGTCC 
AAGAGCATGT CCATTAATAA CATAGCAGGA CAAATGGACG ACACTCCTCT TAAGAAGTTT 
CTGTCCCAGT CAACGGACTC TCTTCACAAG ACCAGTAAAG TCACGGAGTC TACAGAATCT 
CTTACTGATG AGGGTACAGA GATGATGGAC AGCCAGCTGA TGGGAGAATT TGAAGCTGAC 
GCCAAAGAGC TGGAGGCTGA TTCTTATAGC TTCACGGTAG ACAAGAAATT TCTTAAGCAG 
CTTAAAAAGG ATGTTATCAA AAGGCAAGAC GTCATCTATG AGCTCATCCA GACAGAAATG 
CACCATGTAA GGACCTTAAA GATCATGGCT GATGTTTACA GCAAAGGACT ACTGAGAGAA 
GTTCAACTAG AAGCTCAGAC GGTGGAGAAG ATGTTCCCCA TGTTGGACGA TGTTCTGGAT 
TTGCACACAC AGTTCTTTTC ACACCTGCTT GAAAGAAAGA AGGAATCAGG GAGCCAAGAA 
GAGGGCGGCT TTGTCATCCA AAGGATTGGC GATGTGCTGG TTTCTCAGTT CTCAGGTTCC 
AGTGCTGAGA GAATGAAGAA GGTCTATGGG AAGTTCTGCG GCAGGCACAA TGAAGCTGTG 
AACTTCTACA AGGAGCTTCA CACCAAAGAC AAACGCTTTC AAGCTTTCAT CAAGAAAAAG 
ATGAGCAGCC CTGTTGTCCG ACGGCTAAGC ATTCCTGAGT GTATATTGTT AGTCACACAA 
CGAATCACAA AATATCCCGT GTTACTACAG AGAATACTGC AGCACACGAA AGAAAACGAG 
GAGGACCATG CAGACGTTAG TCAAGGTCTG AAGCTGGTGA AGGAGGTGAT TGCTGCCGTG 
GACAATAAAG TGAATGAGCA TGAGAAGAAG AAGAGGCTAA AGGAAGTGTA CAGCCGGACG 
GACAACAAGT CCATCATGAG GATGAAGAGC GGGCAGATGT TTGCCAGAGA GGATCTGTTA 
CGTGGGCGGA AGTTAATTCA CGACGGGCCG CTGCAGCTCA AGAACTCAGC TGGCAGACTC 
AAGGATGTCC AGGCTCTGCT GCTGAGGGAC GTGATTGTGT TCCTGCAGGA GAAAGATCAG 
AAGTACATTT TCGCCTCTTT GGACCAGCGC TCCACCGTCA TCTCCCTGCA GAAGCTCATT 
GTGAGGGAAG TAGCCAATGA GGAGAAAGCT CTGTTTCTGA TCACAGCAGG CATCGAGAAG 
CCCGAGATGG TAGAAGTGTA CACAAGCTCC AAAGAGGAGA GAAACACATG GATGCAGCTC 
ATTCAGGAGG CCATGCAGTC TATACTGTCC ATTTCCTCCA GGGAGAGAGA TGATGATGAA 
GGTGTGCCAA GTGAAAACGA AGAGGACAGG AGACTTCAGG AAATCAAGGT CAAGGAGTTC 
AGAGATCAGC TGCACAAAAG AGACGAGCAG ATCTTGACGT TACTGGAGGA GAAGGTGAAG 
TTGTTCAGGG ACATGTGTGA CTGTGGTGCA CCGGACGAGA CGGGGCTTCG TAATCGAATG 
CTCTTCAGGG CCACGACGGA TGATGTCACC AGAGGAGAGC CAATCATGAA GGAGGCTCTG 
AAGGAAGTTG AAACGCTGCA GGTTCTGGTT AATGACAGTC TTGGCGGTGC GGTTCAGGAA 
GGTGTGTGCG CGGCTGGACA TGTGACTTTG CCACGACGAG CGGAAACATT TGGAGGTTTC 
GACAGTCACC AGATGAACAT CTCTAAGAGC AAAGATGGAG ACAGAGAGGA GCTGGAGGAG 
TCTGCAGAGC TCCGCAGGAC TGAGTCAGAC AGTGTGTTGA AGAAGACGAG TAAGACCAAC 
CCTTTACTGC TGGTCAGGAG AAACAATGAG CAGGTTCTTC AGAGCGTTGC TCATCTTCAT 
GACCTGCTGA ACACATTACA AGCTGTGGTG GTCCAGCAGG ACACCTTCAT CGAAGACCAG 
CGTCACGCTC ATCCCTCCTC ACGCCACCCA TCCATCTCCT CCTCTACCTC CTCTTTATCA 
TCCACCTCAT CCCGGCCGAG CTCTCTAATC GAGCAGGAGA AACAGCGCAG CCTGGAGAAG 
CAGCGGCAGG AGGTGGCCAG CCTCCAAAAA CAGCAGGCGG CCCACGCCGA GGAGAGGCGG 
CGCAGGGAAA AGCAGTGGGA GACCCGAGAG AAAGAGCTAA CCCAGAGGGA GATGCAGATC 
CAGAGCCAAG AAGAGGAGGC GCAGAGGAGA GCCAGACAGC TGGAGGAGGA GAAACAGGAG 
CTGCAGAGTA AGAAAGAGGA GTATCAGAGA GACCTGGCAC GACTGCGGGA CAGCCAGAAG 
AGACTGGAGA GAGAGAGAGA GCAGGTGCAG AGAGAGGCAG ATGAGCTTAA ACTGACTGAG 
GAAGTGACAA AGAACCGGTC TCCGTCAACC ACGTCTGAAG ACTCCACGAA GTTCCAGAGC 
ACCGGTTCAT TAGACCGTGA CCCATCAGAG ATGGAGCTCT CCTCCTCCCC GTCAGCCAGA 
GACTTCCTGT CCCGCATGGA CTCCAAACGC AAAGGGAAAA ACCTCAATCC ATTCTCATCC 
AACTCAAGCC AGAAGAGCCA GAACAGCGAG GCTCAGAGCC AGATGCCCAG CCGTCTGCTC 
CAGCTGGCCA AACCCAAGGA GAAGAAGGAC AAGAAGAAGA AGAAGGGGAA AGGCCAGCAG 
AGCCAGACTG CAGACTCTCA GAGCACTGTT GTTCCAGGCG CTACCCCAGA CGGAGAGATC 
TTTTTCTGCT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_009296108.1
CDS	28..7719
Translation	MKGVPQVASAMKLNPQQAPLYGECLLTVQLCEDECVEDEDDVEFYLLFAGSTQRHVSSTLKISHVTLQAVCPAHDRSETVRVTLCQARPGGSVDPVSEECFQFVQDLALDMAQFLVNAAGKTEGALLLDECQIPLKECERLDDTLALALRHLPLPAGWSVLGTDLDTGTCTGSGIAEPGPHDTLLHFAAKRGLRKVALFLLQQPGGREALRLPNKQGRTPAKVAQKRGHSQLHKLLTEVDNLPELETKTSKWCYPEGRVLQHHPKLNTYTLTLEDVPRSSPPSLQCDVEELQRLIQSHQKEDPPTPHKSALLILNRDSSNSTETDDCLPPNSVEPEHQEHNKQEETCKSLLNSLTFEDGLCVHQNGTGDHVQLLDGDVEPDLPAVGDTGNALDKTDCLQRRVDGDSVAFSAPSCGNQREEADIELCIISAKQGVASQPDKGKQEEQEACQKRSETEKDYNTESGNLGLQDMGHAQSQGLLPTETGQPTQREVESEGSTDVCWRGDLPEEREGDMSSNQEIDSYSSALSSHNLEGNSQHDGETEPQLPTQCSEGTDEAKLTLIQVNSEVTLSIRSEDTVISDSVDKPHGLLETETGNLSEHTKGNTESAADLVADDASVKQNSGFSVLEGDLLESLSLRPNMAPGPVHKVQQGLSPVPETSSHVGPALDPFSETTESFQQPNQSTQLLETALECEDVSLNNSTGDINAIAGVSVDISAEVSLCQNEGCPPDGSKEEVAPSERIPDLKELTEEPESVEDIIVSDVSDLDDKSQPFEESSLPIDSECFIRGDFQNDESTVQSSECTGGDINVGTLLGEEEATEKIEQPDSSLKLVELIPEEYTGDATNENKKIEPEEQSILLPTLQGESGFKDVATEEPVQEREIDSTSEETISDLPDPSEITNNDCAESNAHCQLSESSKESANTSSEPAECVNDLDLSCALPQTEIEGSRPSEGPLIDGVTICAEIPIDDKGVDMATNMSKTTISEDCLSSPMDTTSCSVLESSSMSDPLNASSSTAELTSPLDINSGLHQDLEHTSPVDIDNGLPEEPPPSDTPDCLNGLHQDSPDSLMEHSGTPQPCSEEQCVDQITDDSTSAEDAADGHQDSSVDIANSEAIHRETETFYSVDASQDSVLQEKKSSSVSSEIPSLTPSPESIRHKESPSTMRGSASDGDSLFSQDLGEDSVFNNKAEDSITITSGVSMSYSSSTDDSSSLGTTCATPQTGTEAPASDPFQEQPTSTGASGETEEEEKKDRLTEVPERSAILRTTIRSLSPFRRHSWGPGKNLAGETEMSQRSAAQGEVERRSVGHRRSMSWCPSVVLRSENDEMNERSYSLEGLAPEKEGRKSLVQQRVGESSQEPKCPSKVEKEDRGSLVSLTEEEQESDLGDCSSLGSQKSIQSGRRCVPHSQPILTKSVSMVAISQKDLDAMGRLRPKRRISFSFSISPMLPKSKTLFSLGSSSSDEEEAVRLSSFTGTSGSLEYSISEEDPGPLRSDSEGKIGGTKVSRTFSYLKSKMSKKNKQEKEKEKNKERERDAKDKERRASNGHLFTTVMTVPTIPCHQCNKLINTKDSILCTNCNAQVHKGCKESLPVCAKVKMKQQKQQQTVTDSASTPSVTLRSKTLPARDRPWSTISIPDDQPAPLAPPRKSPSSIMSFNSNPLSKSMSINNIAGQMDDTPLKKFLSQSTDSLHKTSKVTESTESLTDEGTEMMDSQLMGEFEADAKELEADSYSFTVDKKFLKQLKKDVIKRQDVIYELIQTEMHHVRTLKIMADVYSKGLLREVQLEAQTVEKMFPMLDDVLDLHTQFFSHLLERKKESGSQEEGGFVIQRIGDVLVSQFSGSSAERMKKVYGKFCGRHNEAVNFYKELHTKDKRFQAFIKKKMSSPVVRRLSIPECILLVTQRITKYPVLLQRILQHTKENEEDHADVSQGLKLVKEVIAAVDNKVNEHEKKKRLKEVYSRTDNKSIMRMKSGQMFAREDLLRGRKLIHDGPLQLKNSAGRLKDVQALLLRDVIVFLQEKDQKYIFASLDQRSTVISLQKLIVREVANEEKALFLITAGIEKPEMVEVYTSSKEERNTWMQLIQEAMQSILSISSRERDDDEGVPSENEEDRRLQEIKVKEFRDQLHKRDEQILTLLEEKVKLFRDMCDCGAPDETGLRNRMLFRATTDDVTRGEPIMKEALKEVETLQVLVNDSLGGAVQEGVCAAGHVTLPRRAETFGGFDSHQMNISKSKDGDREELEESAELRRTESDSVLKKTSKTNPLLLVRRNNEQVLQSVAHLHDLLNTLQAVVVQQDTFIEDQRHAHPSSRHPSISSSTSSLSSTSSRPSSLIEQEKQRSLEKQRQEVASLQKQQAAHAEERRRREKQWETREKELTQREMQIQSQEEEAQRRARQLEEEKQELQSKKEEYQRDLARLRDSQKRLEREREQVQREADELKLTEEVTKNRSPSTTSEDSTKFQSTGSLDRDPSEMELSSSPSARDFLSRMDSKRKGKNLNPFSSNSSQKSQNSEAQSQMPSRLLQLAKPKEKKDKKKKKGKGQQSQTADSQSTVVPGATPDGEIFFC

Target ORF information:

RefSeq Version	XM_009297833.2
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio wu:fi15d04 (wu:fi15d04), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_009297833.2

ORF Insert Sequence:

ATGAAGGGTG TTCCACAAGT GGCCAGTGCC ATGAAACTGA ATCCACAGCA AGCCCCATTA 
TATGGAGAGT GTCTGCTGAC GGTCCAGCTC TGTGAGGATG AGTGTGTTGA GGATGAGGAT 
GATGTGGAGT TTTACCTGCT CTTTGCTGGA TCCACACAGA GACATGTGTC CAGCACACTG 
AAGATCAGCC ATGTCACGCT GCAGGCCGTC TGTCCAGCTC ATGATCGCAG TGAAACGGTA 
CGGGTCACAC TATGCCAGGC TCGTCCCGGC GGCTCAGTGG ACCCAGTGTC CGAGGAGTGT 
TTCCAGTTTG TCCAGGACCT GGCTCTGGAC ATGGCCCAGT TCCTGGTAAA TGCGGCGGGG 
AAAACAGAAG GAGCGCTGCT GCTGGACGAG TGTCAGATCC CACTGAAAGA GTGCGAGCGG 
CTGGACGATA CTCTTGCTCT GGCTCTCCGG CATCTGCCTT TGCCTGCTGG ATGGAGCGTT 
TTGGGCACAG ATTTGGACAC GGGCACTTGC ACAGGCTCGG GCATCGCAGA GCCTGGACCA 
CACGATACAC TTTTGCACTT CGCTGCGAAG CGCGGACTAC GCAAGGTTGC ACTCTTTCTG 
CTCCAGCAAC CAGGGGGAAG AGAGGCTCTC CGGCTTCCCA ACAAACAGGG TCGCACACCG 
GCCAAGGTCG CCCAGAAGAG GGGACACTCT CAGCTCCACA AACTGCTCAC GGAGGTTGAC 
AATCTGCCCG AGCTGGAGAC GAAAACATCT AAGTGGTGTT ACCCAGAAGG CAGAGTCCTG 
CAACACCACC CCAAGCTAAA CACTTACACC CTTACCCTGG AGGATGTGCC CAGGAGCTCC 
CCACCAAGCC TTCAGTGTGA TGTGGAGGAG CTCCAGCGTC TCATTCAGTC ACATCAGAAG 
GAAGATCCTC CAACACCGCA TAAGTCAGCA CTGCTGATTC TCAATAGAGA CTCCAGTAAT 
AGCACAGAGA CAGATGACTG CCTTCCGCCC AACTCAGTGG AACCTGAGCA TCAGGAGCAC 
AACAAACAAG AAGAAACTTG CAAATCGCTT TTAAACAGCC TTACCTTTGA GGATGGATTG 
TGTGTTCATC AAAACGGCAC AGGAGATCAT GTCCAGTTGC TGGATGGGGA CGTGGAGCCT 
GACCTGCCAG CTGTAGGAGA TACAGGGAAT GCATTAGATA AGACTGACTG TTTGCAGCGG 
CGGGTAGATG GTGATTCTGT GGCTTTCAGT GCCCCTTCCT GTGGAAACCA GCGGGAGGAA 
GCCGATATAG AGCTCTGCAT AATCAGTGCC AAGCAGGGGG TTGCTTCACA GCCTGATAAA 
GGGAAGCAAG AAGAGCAAGA GGCTTGCCAG AAGAGGTCAG AGACTGAAAA GGACTATAAC 
ACTGAATCTG GCAACCTTGG GCTTCAAGAC ATGGGCCACG CACAATCTCA GGGTCTCCTC 
CCCACGGAAA CTGGTCAGCC TACACAAAGG GAGGTGGAGA GTGAGGGGAG TACAGATGTC 
TGTTGGCGTG GGGACTTACC AGAGGAAAGG GAAGGGGACA TGAGTAGCAA CCAGGAAATA 
GACTCATATT CTTCAGCACT TTCTTCCCAT AATCTAGAGG GAAACAGCCA GCACGATGGT 
GAGACTGAGC CCCAGTTGCC TACACAATGC TCAGAGGGAA CAGATGAAGC TAAATTGACT 
TTAATTCAGG TAAATTCTGA AGTTACTTTG TCTATACGAA GCGAGGATAC CGTTATTTCT 
GACAGTGTTG ACAAGCCACA TGGTCTCTTA GAGACGGAGA CAGGAAATCT CAGCGAACAC 
ACCAAAGGCA ACACTGAGTC AGCTGCTGAT TTGGTTGCGG ATGACGCTAG TGTAAAGCAG 
AACTCTGGAT TTAGCGTTTT AGAGGGGGAT CTTTTGGAAT CATTAAGTCT ACGGCCAAAC 
ATGGCACCGG GACCAGTGCA TAAGGTTCAG CAAGGTCTGT CACCAGTTCC TGAGACTTCC 
TCTCATGTAG GTCCAGCTTT GGACCCCTTT TCTGAGACTA CAGAAAGTTT TCAACAGCCG 
AATCAATCCA CACAGCTTTT AGAAACTGCA TTAGAATGTG AGGATGTTTC ATTAAATAAC 
TCAACCGGTG ACATTAATGC AATAGCAGGG GTTTCTGTTG ACATTTCTGC AGAGGTTTCA 
CTTTGCCAGA ATGAAGGGTG TCCTCCTGAT GGAAGCAAAG AAGAGGTAGC CCCCAGTGAG 
AGGATACCAG ACCTTAAAGA GCTTACGGAA GAGCCGGAAT CAGTTGAGGA CATTATTGTC 
TCAGATGTCT CAGATTTGGA TGACAAATCT CAACCTTTTG AAGAGTCTTC TTTGCCTATC 
GACTCTGAAT GTTTTATAAG AGGTGATTTC CAAAACGATG AGTCTACAGT ACAGTCTTCT 
GAATGTACTG GAGGTGACAT AAATGTAGGA ACACTCTTAG GAGAAGAAGA GGCAACTGAA 
AAAATAGAAC AGCCAGACTC TTCACTTAAA CTGGTTGAGC TAATTCCTGA GGAATATACT 
GGAGATGCCA CAAACGAAAA TAAAAAAATT GAGCCAGAGG AACAATCTAT TTTACTGCCA 
ACTTTGCAAG GGGAATCAGG ATTTAAGGAT GTTGCCACAG AGGAACCTGT ACAGGAAAGG 
GAAATTGACT CCACTTCAGA GGAAACAATC AGCGATCTCC CTGATCCATC GGAAATCACA 
AACAATGATT GTGCTGAATC CAATGCACAC TGCCAGCTCT CAGAATCCTC CAAGGAGAGC 
GCAAACACAT CCTCAGAACC TGCTGAATGT GTGAATGACC TAGACTTGTC TTGTGCTTTA 
CCTCAAACAG AAATCGAAGG CAGCAGACCT TCTGAGGGCC CCTTAATTGA TGGAGTAACA 
ATATGTGCTG AAATTCCCAT TGATGACAAA GGTGTTGACA TGGCTACCAA TATGAGTAAG 
ACGACCATCT CTGAGGATTG TCTGTCCTCT CCTATGGACA CCACCAGCTG TTCAGTTCTG 
GAGAGCTCCT CCATGTCTGA CCCACTCAAT GCCTCATCCT CAACAGCTGA GCTCACTTCC 
CCTTTGGATA TTAATAGTGG ATTGCATCAG GATTTGGAAC ACACTTCCCC AGTGGATATA 
GACAATGGTT TGCCTGAGGA ACCGCCGCCG TCAGACACAC CTGATTGCCT GAATGGATTA 
CATCAAGATT CTCCTGATAG TTTGATGGAG CACAGTGGAA CTCCTCAGCC ATGTTCTGAG 
GAACAGTGTG TGGATCAAAT CACAGATGAC AGCACTTCAG CAGAGGATGC AGCGGACGGA 
CACCAAGACT CCTCAGTAGA CATAGCAAAT TCCGAAGCGA TCCACAGAGA GACCGAAACA 
TTTTATTCAG TTGATGCCAG TCAAGACTCT GTACTCCAGG AGAAGAAATC TTCTTCAGTG 
AGCTCAGAAA TCCCCTCTTT GACCCCCTCA CCTGAGAGCA TACGTCACAA AGAGTCGCCA 
AGCACTATGC GAGGGTCCGC CAGCGATGGA GACTCTCTGT TTAGTCAGGA TCTGGGCGAG 
GACAGCGTTT TTAATAATAA GGCTGAGGAC AGCATTACCA TCACCAGTGG CGTGTCCATG 
TCCTATTCGT CCTCCACAGA TGACTCTTCC AGCTTGGGAA CCACTTGCGC CACCCCTCAG 
ACTGGCACAG AGGCTCCTGC ATCAGACCCC TTTCAGGAAC AGCCCACATC TACTGGAGCT 
TCTGGGGAAA CAGAGGAGGA AGAGAAAAAG GACCGTCTCA CTGAGGTTCC GGAGCGATCG 
GCCATTTTAC GGACCACCAT ACGATCACTG TCCCCTTTTC GAAGACACAG TTGGGGGCCG 
GGCAAAAATT TGGCAGGGGA GACAGAGATG AGTCAGCGCA GTGCGGCACA AGGTGAAGTG 
GAGCGCAGAT CTGTTGGTCA TAGGAGAAGC ATGAGCTGGT GTCCTTCTGT TGTCCTGCGC 
TCTGAAAATG ATGAAATGAA TGAAAGAAGT TACAGTCTGG AAGGACTTGC ACCAGAGAAG 
GAGGGAAGGA AAAGTCTGGT TCAGCAGCGT GTCGGAGAGT CCAGCCAGGA GCCCAAATGT 
CCATCAAAGG TGGAAAAAGA AGACAGAGGC TCGCTTGTGT CTCTGACTGA AGAAGAGCAG 
GAGTCAGATC TAGGCGACTG CAGCAGTTTG GGCAGTCAGA AGTCCATCCA GTCGGGTCGG 
AGATGTGTCC CACATTCACA ACCCATCCTC ACCAAATCTG TGTCCATGGT GGCCATCAGT 
CAGAAGGACC TGGACGCAAT GGGACGGCTG CGACCCAAGC GACGAATCTC CTTCTCCTTC 
AGCATCTCGC CGATGCTCCC CAAATCTAAA ACTCTCTTTT CTCTTGGCTC TTCATCCAGT 
GATGAGGAGG AGGCAGTCAG ATTGAGTTCA TTCACTGGAA CATCAGGATC ATTAGAGTAC 
AGTATATCAG AGGAGGATCC TGGGCCATTA CGGAGTGACA GTGAAGGCAA AATTGGAGGA 
ACCAAAGTGA GCAGGACATT CAGTTACCTC AAGAGCAAGA TGAGCAAGAA GAACAAGCAG 
GAGAAGGAGA AAGAGAAGAA TAAGGAGCGT GAGCGAGATG CCAAAGACAA GGAGAGAAGA 
GCCTCAAACG GACATCTCTT CACTACGGTC ATGACTGTAC CCACAATCCC CTGCCACCAG 
TGCAACAAAC TCATCAACAC TAAAGACTCT ATCCTCTGCA CCAATTGCAA TGCACAAGTC 
CACAAAGGCT GCAAAGAGAG TCTTCCTGTC TGCGCTAAAG TCAAGATGAA GCAGCAAAAA 
CAGCAGCAGA CTGTTACAGA TTCTGCCTCG ACGCCTAGTG TCACCCTCAG AAGTAAAACT 
CTTCCAGCAA GGGATCGTCC GTGGTCCACA ATATCCATTC CCGATGACCA GCCGGCTCCC 
CTCGCTCCTC CTCGGAAAAG CCCCAGCAGC ATCATGTCTT TCAACAGCAA CCCACTGTCC 
AAGAGCATGT CCATTAATAA CATAGCAGGA CAAATGGACG ACACTCCTCT TAAGAAGTTT 
CTGTCCCAGT CAACGGACTC TCTTCACAAG ACCAGTAAAG TCACGGAGTC TACAGAATCT 
CTTACTGATG AGGGTACAGA GATGATGGAC AGCCAGCTGA TGGGAGAATT TGAAGCTGAC 
GCCAAAGAGC TGGAGGCTGA TTCTTATAGC TTCACGGTAG ACAAGAAATT TCTTAAGCAG 
CTTAAAAAGG ATGTTATCAA AAGGCAAGAC GTCATCTATG AGCTCATCCA GACAGAAATG 
CACCATGTAA GGACCTTAAA GATCATGGCT GATGTTTACA GCAAAGGACT ACTGAGAGAA 
GTTCAACTAG AAGCTCAGAC GGTGGAGAAG ATGTTCCCCA TGTTGGACGA TGTTCTGGAT 
TTGCACACAC AGTTCTTTTC ACACCTGCTT GAAAGAAAGA AGGAATCAGG GAGCCAAGAA 
GAGGGCGGCT TTGTCATCCA AAGGATTGGC GATGTGCTGG TTTCTCAGTT CTCAGGTTCC 
AGTGCTGAGA GAATGAAGAA GGTCTATGGG AAGTTCTGCG GCAGGCACAA TGAAGCTGTG 
AACTTCTACA AGGAGCTTCA CACCAAAGAC AAACGCTTTC AAGCTTTCAT CAAGAAAAAG 
ATGAGCAGCC CTGTTGTCCG ACGGCTAAGC ATTCCTGAGT GTATATTGTT AGTCACACAA 
CGAATCACAA AATATCCCGT GTTACTACAG AGAATACTGC AGCACACGAA AGAAAACGAG 
GAGGACCATG CAGACGTTAG TCAAGGTCTG AAGCTGGTGA AGGAGGTGAT TGCTGCCGTG 
GACAATAAAG TGAATGAGCA TGAGAAGAAG AAGAGGCTAA AGGAAGTGTA CAGCCGGACG 
GACAACAAGT CCATCATGAG GATGAAGAGC GGGCAGATGT TTGCCAGAGA GGATCTGTTA 
CGTGGGCGGA AGTTAATTCA CGACGGGCCG CTGCAGCTCA AGAACTCAGC TGGCAGACTC 
AAGGATGTCC AGGCTCTGCT GCTGAGGGAC GTGATTGTGT TCCTGCAGGA GAAAGATCAG 
AAGTACATTT TCGCCTCTTT GGACCAGCGC TCCACCGTCA TCTCCCTGCA GAAGCTCATT 
GTGAGGGAAG TAGCCAATGA GGAGAAAGCT CTGTTTCTGA TCACAGCAGG CATCGAGAAG 
CCCGAGATGG TAGAAGTGTA CACAAGCTCC AAAGAGGAGA GAAACACATG GATGCAGCTC 
ATTCAGGAGG CCATGCAGTC TATACTGTCC ATTTCCTCCA GGGAGAGAGA TGATGATGAA 
GGTGTGCCAA GTGAAAACGA AGAGGACAGG AGACTTCAGG AAATCAAGGT CAAGGAGTTC 
AGAGATCAGC TGCACAAAAG AGACGAGCAG ATCTTGACGT TACTGGAGGA GAAGGTGAAG 
TTGTTCAGGG ACATGTGTGA CTGTGGTGCA CCGGACGAGA CGGGGCTTCG TAATCGAATG 
CTCTTCAGGG CCACGACGGA TGATGTCACC AGAGGAGAGC CAATCATGAA GGAGGCTCTG 
AAGGAAGTTG AAACGCTGCA GGTTCTGGTT AATGACAGTC TTGGCGGTGC GGTTCAGGAA 
GGTGTGTGCG CGGCTGGACA TGTGACTTTG CCACGACGAG CGGAAACATT TGGAGGTTTC 
GACAGTCACC AGATGAACAT CTCTAAGAGC AAAGATGGAG ACAGAGAGGA GCTGGAGGAG 
TCTGCAGAGC TCCGCAGGAC TGAGTCAGAC AGTGTGTTGA AGAAGACGAG TAAGACCAAC 
CCTTTACTGC TGGTCAGGAG AAACAATGAG CAGGTTCTTC AGAGCGTTGC TCATCTTCAT 
GACCTGCTGA ACACATTACA AGCTGTGGTG GTCCAGCAGG ACACCTTCAT CGAAGACCAG 
CGTCACGCTC ATCCCTCCTC ACGCCACCCA TCCATCTCCT CCTCTACCTC CTCTTTATCA 
TCCACCTCAT CCCGGCCGAG CTCTCTAATC GAGCAGGAGA AACAGCGCAG CCTGGAGAAG 
CAGCGGCAGG AGGTGGCCAG CCTCCAAAAA CAGCAGGCGG CCCACGCCGA GGAGAGGCGG 
CGCAGGGAAA AGCAGTGGGA GACCCGAGAG AAAGAGCTAA CCCAGAGGGA GATGCAGATC 
CAGAGCCAAG AAGAGGAGGC GCAGAGGAGA GCCAGACAGC TGGAGGAGGA GAAACAGGAG 
CTGCAGAGTA AGAAAGAGGA GTATCAGAGA GACCTGGCAC GACTGCGGGA CAGCCAGAAG 
AGACTGGAGA GAGAGAGAGA GCAGGTGCAG AGAGAGGCAG ATGAGCTTAA ACTGACTGAG 
GAAGTGACAA AGAACCGGTC TCCGTCAACC ACGTCTGAAG ACTCCACGAA GTTCCAGAGC 
ACCGGTTCAT TAGACCGTGA CCCATCAGAG ATGGAGCTCT CCTCCTCCCC GTCAGCCAGA 
GACTTCCTGT CCCGCATGGA CTCCAAACGC AAAGGGAAAA ACCTCAATCC ATTCTCATCC 
AACTCAAGCC AGAAGAGCCA GAACAGCGAG GCTCAGAGCC AGATGCCCAG CCGTCTGCTC 
CAGCTGGCCA AACCCAAGGA GAAGAAGGAC AAGAAGAAGA AGAAGGGGAA AGGCCAGCAG 
AGCCAGACTG CAGACTCTCA GAGCACTGTT GTTCCAGGCG CTACCCCAGA CGGAGAGATC 
TTTTTCTGCT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

wu:fi15d04 ( XM_009297834.2 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_009296109.1 Danio rerio wu:fi15d04 (wu:fi15d04), transcript variant X2, mRNA.

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CloneID	ODa39928
Clone ID Related Accession (Same CDS sequence)	XM_009297834.2
Accession Version	XM_009297834.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7689bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-23
Organism	Danio rerio(Zebrafish)
Product	A-kinase anchor protein 13 isoform X2
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007136.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_009297834.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAGGGTG TTCCACAAGT GGCCAGTGCC ATGAAACTGA ATCCACAGCA AGCCCCATTA 
TATGGAGAGT GTCTGCTGAC GGTCCAGCTC TGTGAGGATG AGTGTGTTGA GGATGAGGAT 
GATGTGGAGT TTTACCTGCT CTTTGCTGGA TCCACACAGA GACATGTGTC CAGCACACTG 
AAGATCAGCC ATGTCACGCT GCAGGCCGTC TGTCCAGCTC ATGATCGCAG TGAAACGGTA 
CGGGTCACAC TATGCCAGGC TCGTCCCGGC GGCTCAGTGG ACCCAGTGTC CGAGGAGTGT 
TTCCAGTTTG TCCAGGACCT GGCTCTGGAC ATGGCCCAGT TCCTGGTAAA TGCGGCGGGG 
AAAACAGAAG GAGCGCTGCT GCTGGACGAG TGTCAGATCC CACTGAAAGA GTGCGAGCGG 
CTGGACGATA CTCTTGCTCT GGCTCTCCGG CATCTGCCTT TGCCTGCTGG ATGGAGCGTT 
TTGGGCACAG ATTTGGACAC GGGCACTTGC ACAGGCTCGG GCATCGAGCC TGGACCACAC 
GATACACTTT TGCACTTCGC TGCGAAGCGC GGACTACGCA AGGTTGCACT CTTTCTGCTC 
CAGCAACCAG GGGGAAGAGA GGCTCTCCGG CTTCCCAACA AACAGGGTCG CACACCGGCC 
AAGGTCGCCC AGAAGAGGGG ACACTCTCAG CTCCACAAAC TGCTCACGGA GGTTGACAAT 
CTGCCCGAGC TGGAGACGAA AACATCTAAG TGGTGTTACC CAGAAGGCAG AGTCCTGCAA 
CACCACCCCA AGCTAAACAC TTACACCCTT ACCCTGGAGG ATGTGCCCAG GAGCTCCCCA 
CCAAGCCTTC AGTGTGATGT GGAGGAGCTC CAGCGTCTCA TTCAGTCACA TCAGAAGGAA 
GATCCTCCAA CACCGCATAA GTCAGCACTG CTGATTCTCA ATAGAGACTC CAGTAATAGC 
ACAGAGACAG ATGACTGCCT TCCGCCCAAC TCAGTGGAAC CTGAGCATCA GGAGCACAAC 
AAACAAGAAG AAACTTGCAA ATCGCTTTTA AACAGCCTTA CCTTTGAGGA TGGATTGTGT 
GTTCATCAAA ACGGCACAGG AGATCATGTC CAGTTGCTGG ATGGGGACGT GGAGCCTGAC 
CTGCCAGCTG TAGGAGATAC AGGGAATGCA TTAGATAAGA CTGACTGTTT GCAGCGGCGG 
GTAGATGGTG ATTCTGTGGC TTTCAGTGCC CCTTCCTGTG GAAACCAGCG GGAGGAAGCC 
GATATAGAGC TCTGCATAAT CAGTGCCAAG CAGGGGGTTG CTTCACAGCC TGATAAAGGG 
AAGCAAGAAG AGCAAGAGGC TTGCCAGAAG AGGTCAGAGA CTGAAAAGGA CTATAACACT 
GAATCTGGCA ACCTTGGGCT TCAAGACATG GGCCACGCAC AATCTCAGGG TCTCCTCCCC 
ACGGAAACTG GTCAGCCTAC ACAAAGGGAG GTGGAGAGTG AGGGGAGTAC AGATGTCTGT 
TGGCGTGGGG ACTTACCAGA GGAAAGGGAA GGGGACATGA GTAGCAACCA GGAAATAGAC 
TCATATTCTT CAGCACTTTC TTCCCATAAT CTAGAGGGAA ACAGCCAGCA CGATGGTGAG 
ACTGAGCCCC AGTTGCCTAC ACAATGCTCA GAGGGAACAG ATGAAGCTAA ATTGACTTTA 
ATTCAGGTAA ATTCTGAAGT TACTTTGTCT ATACGAAGCG AGGATACCGT TATTTCTGAC 
AGTGTTGACA AGCCACATGG TCTCTTAGAG ACGGAGACAG GAAATCTCAG CGAACACACC 
AAAGGCAACA CTGAGTCAGC TGCTGATTTG GTTGCGGATG ACGCTAGTGT AAAGCAGAAC 
TCTGGATTTA GCGTTTTAGA GGGGGATCTT TTGGAATCAT TAAGTCTACG GCCAAACATG 
GCACCGGGAC CAGTGCATAA GGTTCAGCAA GGTCTGTCAC CAGTTCCTGA GACTTCCTCT 
CATGTAGGTC CAGCTTTGGA CCCCTTTTCT GAGACTACAG AAAGTTTTCA ACAGCCGAAT 
CAATCCACAC AGCTTTTAGA AACTGCATTA GAATGTGAGG ATGTTTCATT AAATAACTCA 
ACCGGTGACA TTAATGCAAT AGCAGGGGTT TCTGTTGACA TTTCTGCAGA GGTTTCACTT 
TGCCAGAATG AAGGGTGTCC TCCTGATGGA AGCAAAGAAG AGGTAGCCCC CAGTGAGAGG 
ATACCAGACC TTAAAGAGCT TACGGAAGAG CCGGAATCAG TTGAGGACAT TATTGTCTCA 
GATGTCTCAG ATTTGGATGA CAAATCTCAA CCTTTTGAAG AGTCTTCTTT GCCTATCGAC 
TCTGAATGTT TTATAAGAGG TGATTTCCAA AACGATGAGT CTACAGTACA GTCTTCTGAA 
TGTACTGGAG GTGACATAAA TGTAGGAACA CTCTTAGGAG AAGAAGAGGC AACTGAAAAA 
ATAGAACAGC CAGACTCTTC ACTTAAACTG GTTGAGCTAA TTCCTGAGGA ATATACTGGA 
GATGCCACAA ACGAAAATAA AAAAATTGAG CCAGAGGAAC AATCTATTTT ACTGCCAACT 
TTGCAAGGGG AATCAGGATT TAAGGATGTT GCCACAGAGG AACCTGTACA GGAAAGGGAA 
ATTGACTCCA CTTCAGAGGA AACAATCAGC GATCTCCCTG ATCCATCGGA AATCACAAAC 
AATGATTGTG CTGAATCCAA TGCACACTGC CAGCTCTCAG AATCCTCCAA GGAGAGCGCA 
AACACATCCT CAGAACCTGC TGAATGTGTG AATGACCTAG ACTTGTCTTG TGCTTTACCT 
CAAACAGAAA TCGAAGGCAG CAGACCTTCT GAGGGCCCCT TAATTGATGG AGTAACAATA 
TGTGCTGAAA TTCCCATTGA TGACAAAGGT GTTGACATGG CTACCAATAT GAGTAAGACG 
ACCATCTCTG AGGATTGTCT GTCCTCTCCT ATGGACACCA CCAGCTGTTC AGTTCTGGAG 
AGCTCCTCCA TGTCTGACCC ACTCAATGCC TCATCCTCAA CAGCTGAGCT CACTTCCCCT 
TTGGATATTA ATAGTGGATT GCATCAGGAT TTGGAACACA CTTCCCCAGT GGATATAGAC 
AATGGTTTGC CTGAGGAACC GCCGCCGTCA GACACACCTG ATTGCCTGAA TGGATTACAT 
CAAGATTCTC CTGATAGTTT GATGGAGCAC AGTGGAACTC CTCAGCCATG TTCTGAGGAA 
CAGTGTGTGG ATCAAATCAC AGATGACAGC ACTTCAGCAG AGGATGCAGC GGACGGACAC 
CAAGACTCCT CAGTAGACAT AGCAAATTCC GAAGCGATCC ACAGAGAGAC CGAAACATTT 
TATTCAGTTG ATGCCAGTCA AGACTCTGTA CTCCAGGAGA AGAAATCTTC TTCAGTGAGC 
TCAGAAATCC CCTCTTTGAC CCCCTCACCT GAGAGCATAC GTCACAAAGA GTCGCCAAGC 
ACTATGCGAG GGTCCGCCAG CGATGGAGAC TCTCTGTTTA GTCAGGATCT GGGCGAGGAC 
AGCGTTTTTA ATAATAAGGC TGAGGACAGC ATTACCATCA CCAGTGGCGT GTCCATGTCC 
TATTCGTCCT CCACAGATGA CTCTTCCAGC TTGGGAACCA CTTGCGCCAC CCCTCAGACT 
GGCACAGAGG CTCCTGCATC AGACCCCTTT CAGGAACAGC CCACATCTAC TGGAGCTTCT 
GGGGAAACAG AGGAGGAAGA GAAAAAGGAC CGTCTCACTG AGGTTCCGGA GCGATCGGCC 
ATTTTACGGA CCACCATACG ATCACTGTCC CCTTTTCGAA GACACAGTTG GGGGCCGGGC 
AAAAATTTGG CAGGGGAGAC AGAGATGAGT CAGCGCAGTG CGGCACAAGG TGAAGTGGAG 
CGCAGATCTG TTGGTCATAG GAGAAGCATG AGCTGGTGTC CTTCTGTTGT CCTGCGCTCT 
GAAAATGATG AAATGAATGA AAGAAGTTAC AGTCTGGAAG GACTTGCACC AGAGAAGGAG 
GGAAGGAAAA GTCTGGTTCA GCAGCGTGTC GGAGAGTCCA GCCAGGAGCC CAAATGTCCA 
TCAAAGGTGG AAAAAGAAGA CAGAGGCTCG CTTGTGTCTC TGACTGAAGA AGAGCAGGAG 
TCAGATCTAG GCGACTGCAG CAGTTTGGGC AGTCAGAAGT CCATCCAGTC GGGTCGGAGA 
TGTGTCCCAC ATTCACAACC CATCCTCACC AAATCTGTGT CCATGGTGGC CATCAGTCAG 
AAGGACCTGG ACGCAATGGG ACGGCTGCGA CCCAAGCGAC GAATCTCCTT CTCCTTCAGC 
ATCTCGCCGA TGCTCCCCAA ATCTAAAACT CTCTTTTCTC TTGGCTCTTC ATCCAGTGAT 
GAGGAGGAGG CAGTCAGATT GAGTTCATTC ACTGGAACAT CAGGATCATT AGAGTACAGT 
ATATCAGAGG AGGATCCTGG GCCATTACGG AGTGACAGTG AAGGCAAAAT TGGAGGAACC 
AAAGTGAGCA GGACATTCAG TTACCTCAAG AGCAAGATGA GCAAGAAGAA CAAGCAGGAG 
AAGGAGAAAG AGAAGAATAA GGAGCGTGAG CGAGATGCCA AAGACAAGGA GAGAAGAGCC 
TCAAACGGAC ATCTCTTCAC TACGGTCATG ACTGTACCCA CAATCCCCTG CCACCAGTGC 
AACAAACTCA TCAACACTAA AGACTCTATC CTCTGCACCA ATTGCAATGC ACAAGTCCAC 
AAAGGCTGCA AAGAGAGTCT TCCTGTCTGC GCTAAAGTCA AGATGAAGCA GCAAAAACAG 
CAGCAGACTG TTACAGATTC TGCCTCGACG CCTAGTGTCA CCCTCAGAAG TAAAACTCTT 
CCAGCAAGGG ATCGTCCGTG GTCCACAATA TCCATTCCCG ATGACCAGCC GGCTCCCCTC 
GCTCCTCCTC GGAAAAGCCC CAGCAGCATC ATGTCTTTCA ACAGCAACCC ACTGTCCAAG 
AGCATGTCCA TTAATAACAT AGCAGGACAA ATGGACGACA CTCCTCTTAA GAAGTTTCTG 
TCCCAGTCAA CGGACTCTCT TCACAAGACC AGTAAAGTCA CGGAGTCTAC AGAATCTCTT 
ACTGATGAGG GTACAGAGAT GATGGACAGC CAGCTGATGG GAGAATTTGA AGCTGACGCC 
AAAGAGCTGG AGGCTGATTC TTATAGCTTC ACGGTAGACA AGAAATTTCT TAAGCAGCTT 
AAAAAGGATG TTATCAAAAG GCAAGACGTC ATCTATGAGC TCATCCAGAC AGAAATGCAC 
CATGTAAGGA CCTTAAAGAT CATGGCTGAT GTTTACAGCA AAGGACTACT GAGAGAAGTT 
CAACTAGAAG CTCAGACGGT GGAGAAGATG TTCCCCATGT TGGACGATGT TCTGGATTTG 
CACACACAGT TCTTTTCACA CCTGCTTGAA AGAAAGAAGG AATCAGGGAG CCAAGAAGAG 
GGCGGCTTTG TCATCCAAAG GATTGGCGAT GTGCTGGTTT CTCAGTTCTC AGGTTCCAGT 
GCTGAGAGAA TGAAGAAGGT CTATGGGAAG TTCTGCGGCA GGCACAATGA AGCTGTGAAC 
TTCTACAAGG AGCTTCACAC CAAAGACAAA CGCTTTCAAG CTTTCATCAA GAAAAAGATG 
AGCAGCCCTG TTGTCCGACG GCTAAGCATT CCTGAGTGTA TATTGTTAGT CACACAACGA 
ATCACAAAAT ATCCCGTGTT ACTACAGAGA ATACTGCAGC ACACGAAAGA AAACGAGGAG 
GACCATGCAG ACGTTAGTCA AGGTCTGAAG CTGGTGAAGG AGGTGATTGC TGCCGTGGAC 
AATAAAGTGA ATGAGCATGA GAAGAAGAAG AGGCTAAAGG AAGTGTACAG CCGGACGGAC 
AACAAGTCCA TCATGAGGAT GAAGAGCGGG CAGATGTTTG CCAGAGAGGA TCTGTTACGT 
GGGCGGAAGT TAATTCACGA CGGGCCGCTG CAGCTCAAGA ACTCAGCTGG CAGACTCAAG 
GATGTCCAGG CTCTGCTGCT GAGGGACGTG ATTGTGTTCC TGCAGGAGAA AGATCAGAAG 
TACATTTTCG CCTCTTTGGA CCAGCGCTCC ACCGTCATCT CCCTGCAGAA GCTCATTGTG 
AGGGAAGTAG CCAATGAGGA GAAAGCTCTG TTTCTGATCA CAGCAGGCAT CGAGAAGCCC 
GAGATGGTAG AAGTGTACAC AAGCTCCAAA GAGGAGAGAA ACACATGGAT GCAGCTCATT 
CAGGAGGCCA TGCAGTCTAT ACTGTCCATT TCCTCCAGGG AGAGAGATGA TGATGAAGGT 
GTGCCAAGTG AAAACGAAGA GGACAGGAGA CTTCAGGAAA TCAAGGTCAA GGAGTTCAGA 
GATCAGCTGC ACAAAAGAGA CGAGCAGATC TTGACGTTAC TGGAGGAGAA GGTGAAGTTG 
TTCAGGGACA TGTGTGACTG TGGTGCACCG GACGAGACGG GGCTTCGTAA TCGAATGCTC 
TTCAGGGCCA CGACGGATGA TGTCACCAGA GGAGAGCCAA TCATGAAGGA GGCTCTGAAG 
GAAGTTGAAA CGCTGCAGGT TCTGGTTAAT GACAGTCTTG GCGGTGCGGT TCAGGAAGGT 
GTGTGCGCGG CTGGACATGT GACTTTGCCA CGACGAGCGG AAACATTTGG AGGTTTCGAC 
AGTCACCAGA TGAACATCTC TAAGAGCAAA GATGGAGACA GAGAGGAGCT GGAGGAGTCT 
GCAGAGCTCC GCAGGACTGA GTCAGACAGT GTGTTGAAGA AGACGAGTAA GACCAACCCT 
TTACTGCTGG TCAGGAGAAA CAATGAGCAG GTTCTTCAGA GCGTTGCTCA TCTTCATGAC 
CTGCTGAACA CATTACAAGC TGTGGTGGTC CAGCAGGACA CCTTCATCGA AGACCAGCGT 
CACGCTCATC CCTCCTCACG CCACCCATCC ATCTCCTCCT CTACCTCCTC TTTATCATCC 
ACCTCATCCC GGCCGAGCTC TCTAATCGAG CAGGAGAAAC AGCGCAGCCT GGAGAAGCAG 
CGGCAGGAGG TGGCCAGCCT CCAAAAACAG CAGGCGGCCC ACGCCGAGGA GAGGCGGCGC 
AGGGAAAAGC AGTGGGAGAC CCGAGAGAAA GAGCTAACCC AGAGGGAGAT GCAGATCCAG 
AGCCAAGAAG AGGAGGCGCA GAGGAGAGCC AGACAGCTGG AGGAGGAGAA ACAGGAGCTG 
CAGAGTAAGA AAGAGGAGTA TCAGAGAGAC CTGGCACGAC TGCGGGACAG CCAGAAGAGA 
CTGGAGAGAG AGAGAGAGCA GGTGCAGAGA GAGGCAGATG AGCTTAAACT GACTGAGGAA 
GTGACAAAGA ACCGGTCTCC GTCAACCACG TCTGAAGACT CCACGAAGTT CCAGAGCACC 
GGTTCATTAG ACCGTGACCC ATCAGAGATG GAGCTCTCCT CCTCCCCGTC AGCCAGAGAC 
TTCCTGTCCC GCATGGACTC CAAACGCAAA GGGAAAAACC TCAATCCATT CTCATCCAAC 
TCAAGCCAGA AGAGCCAGAA CAGCGAGGCT CAGAGCCAGA TGCCCAGCCG TCTGCTCCAG 
CTGGCCAAAC CCAAGGAGAA GAAGGACAAG AAGAAGAAGA AGGGGAAAGG CCAGCAGAGC 
CAGACTGCAG ACTCTCAGAG CACTGTTGTT CCAGGCGCTA CCCCAGACGG AGAGATCTTT 
TTCTGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_009296109.1
CDS	28..7716
Translation	MKGVPQVASAMKLNPQQAPLYGECLLTVQLCEDECVEDEDDVEFYLLFAGSTQRHVSSTLKISHVTLQAVCPAHDRSETVRVTLCQARPGGSVDPVSEECFQFVQDLALDMAQFLVNAAGKTEGALLLDECQIPLKECERLDDTLALALRHLPLPAGWSVLGTDLDTGTCTGSGIEPGPHDTLLHFAAKRGLRKVALFLLQQPGGREALRLPNKQGRTPAKVAQKRGHSQLHKLLTEVDNLPELETKTSKWCYPEGRVLQHHPKLNTYTLTLEDVPRSSPPSLQCDVEELQRLIQSHQKEDPPTPHKSALLILNRDSSNSTETDDCLPPNSVEPEHQEHNKQEETCKSLLNSLTFEDGLCVHQNGTGDHVQLLDGDVEPDLPAVGDTGNALDKTDCLQRRVDGDSVAFSAPSCGNQREEADIELCIISAKQGVASQPDKGKQEEQEACQKRSETEKDYNTESGNLGLQDMGHAQSQGLLPTETGQPTQREVESEGSTDVCWRGDLPEEREGDMSSNQEIDSYSSALSSHNLEGNSQHDGETEPQLPTQCSEGTDEAKLTLIQVNSEVTLSIRSEDTVISDSVDKPHGLLETETGNLSEHTKGNTESAADLVADDASVKQNSGFSVLEGDLLESLSLRPNMAPGPVHKVQQGLSPVPETSSHVGPALDPFSETTESFQQPNQSTQLLETALECEDVSLNNSTGDINAIAGVSVDISAEVSLCQNEGCPPDGSKEEVAPSERIPDLKELTEEPESVEDIIVSDVSDLDDKSQPFEESSLPIDSECFIRGDFQNDESTVQSSECTGGDINVGTLLGEEEATEKIEQPDSSLKLVELIPEEYTGDATNENKKIEPEEQSILLPTLQGESGFKDVATEEPVQEREIDSTSEETISDLPDPSEITNNDCAESNAHCQLSESSKESANTSSEPAECVNDLDLSCALPQTEIEGSRPSEGPLIDGVTICAEIPIDDKGVDMATNMSKTTISEDCLSSPMDTTSCSVLESSSMSDPLNASSSTAELTSPLDINSGLHQDLEHTSPVDIDNGLPEEPPPSDTPDCLNGLHQDSPDSLMEHSGTPQPCSEEQCVDQITDDSTSAEDAADGHQDSSVDIANSEAIHRETETFYSVDASQDSVLQEKKSSSVSSEIPSLTPSPESIRHKESPSTMRGSASDGDSLFSQDLGEDSVFNNKAEDSITITSGVSMSYSSSTDDSSSLGTTCATPQTGTEAPASDPFQEQPTSTGASGETEEEEKKDRLTEVPERSAILRTTIRSLSPFRRHSWGPGKNLAGETEMSQRSAAQGEVERRSVGHRRSMSWCPSVVLRSENDEMNERSYSLEGLAPEKEGRKSLVQQRVGESSQEPKCPSKVEKEDRGSLVSLTEEEQESDLGDCSSLGSQKSIQSGRRCVPHSQPILTKSVSMVAISQKDLDAMGRLRPKRRISFSFSISPMLPKSKTLFSLGSSSSDEEEAVRLSSFTGTSGSLEYSISEEDPGPLRSDSEGKIGGTKVSRTFSYLKSKMSKKNKQEKEKEKNKERERDAKDKERRASNGHLFTTVMTVPTIPCHQCNKLINTKDSILCTNCNAQVHKGCKESLPVCAKVKMKQQKQQQTVTDSASTPSVTLRSKTLPARDRPWSTISIPDDQPAPLAPPRKSPSSIMSFNSNPLSKSMSINNIAGQMDDTPLKKFLSQSTDSLHKTSKVTESTESLTDEGTEMMDSQLMGEFEADAKELEADSYSFTVDKKFLKQLKKDVIKRQDVIYELIQTEMHHVRTLKIMADVYSKGLLREVQLEAQTVEKMFPMLDDVLDLHTQFFSHLLERKKESGSQEEGGFVIQRIGDVLVSQFSGSSAERMKKVYGKFCGRHNEAVNFYKELHTKDKRFQAFIKKKMSSPVVRRLSIPECILLVTQRITKYPVLLQRILQHTKENEEDHADVSQGLKLVKEVIAAVDNKVNEHEKKKRLKEVYSRTDNKSIMRMKSGQMFAREDLLRGRKLIHDGPLQLKNSAGRLKDVQALLLRDVIVFLQEKDQKYIFASLDQRSTVISLQKLIVREVANEEKALFLITAGIEKPEMVEVYTSSKEERNTWMQLIQEAMQSILSISSRERDDDEGVPSENEEDRRLQEIKVKEFRDQLHKRDEQILTLLEEKVKLFRDMCDCGAPDETGLRNRMLFRATTDDVTRGEPIMKEALKEVETLQVLVNDSLGGAVQEGVCAAGHVTLPRRAETFGGFDSHQMNISKSKDGDREELEESAELRRTESDSVLKKTSKTNPLLLVRRNNEQVLQSVAHLHDLLNTLQAVVVQQDTFIEDQRHAHPSSRHPSISSSTSSLSSTSSRPSSLIEQEKQRSLEKQRQEVASLQKQQAAHAEERRRREKQWETREKELTQREMQIQSQEEEAQRRARQLEEEKQELQSKKEEYQRDLARLRDSQKRLEREREQVQREADELKLTEEVTKNRSPSTTSEDSTKFQSTGSLDRDPSEMELSSSPSARDFLSRMDSKRKGKNLNPFSSNSSQKSQNSEAQSQMPSRLLQLAKPKEKKDKKKKKGKGQQSQTADSQSTVVPGATPDGEIFFC

Target ORF information:

RefSeq Version	XM_009297834.2
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio wu:fi15d04 (wu:fi15d04), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_009297834.2

ORF Insert Sequence:

ATGAAGGGTG TTCCACAAGT GGCCAGTGCC ATGAAACTGA ATCCACAGCA AGCCCCATTA 
TATGGAGAGT GTCTGCTGAC GGTCCAGCTC TGTGAGGATG AGTGTGTTGA GGATGAGGAT 
GATGTGGAGT TTTACCTGCT CTTTGCTGGA TCCACACAGA GACATGTGTC CAGCACACTG 
AAGATCAGCC ATGTCACGCT GCAGGCCGTC TGTCCAGCTC ATGATCGCAG TGAAACGGTA 
CGGGTCACAC TATGCCAGGC TCGTCCCGGC GGCTCAGTGG ACCCAGTGTC CGAGGAGTGT 
TTCCAGTTTG TCCAGGACCT GGCTCTGGAC ATGGCCCAGT TCCTGGTAAA TGCGGCGGGG 
AAAACAGAAG GAGCGCTGCT GCTGGACGAG TGTCAGATCC CACTGAAAGA GTGCGAGCGG 
CTGGACGATA CTCTTGCTCT GGCTCTCCGG CATCTGCCTT TGCCTGCTGG ATGGAGCGTT 
TTGGGCACAG ATTTGGACAC GGGCACTTGC ACAGGCTCGG GCATCGAGCC TGGACCACAC 
GATACACTTT TGCACTTCGC TGCGAAGCGC GGACTACGCA AGGTTGCACT CTTTCTGCTC 
CAGCAACCAG GGGGAAGAGA GGCTCTCCGG CTTCCCAACA AACAGGGTCG CACACCGGCC 
AAGGTCGCCC AGAAGAGGGG ACACTCTCAG CTCCACAAAC TGCTCACGGA GGTTGACAAT 
CTGCCCGAGC TGGAGACGAA AACATCTAAG TGGTGTTACC CAGAAGGCAG AGTCCTGCAA 
CACCACCCCA AGCTAAACAC TTACACCCTT ACCCTGGAGG ATGTGCCCAG GAGCTCCCCA 
CCAAGCCTTC AGTGTGATGT GGAGGAGCTC CAGCGTCTCA TTCAGTCACA TCAGAAGGAA 
GATCCTCCAA CACCGCATAA GTCAGCACTG CTGATTCTCA ATAGAGACTC CAGTAATAGC 
ACAGAGACAG ATGACTGCCT TCCGCCCAAC TCAGTGGAAC CTGAGCATCA GGAGCACAAC 
AAACAAGAAG AAACTTGCAA ATCGCTTTTA AACAGCCTTA CCTTTGAGGA TGGATTGTGT 
GTTCATCAAA ACGGCACAGG AGATCATGTC CAGTTGCTGG ATGGGGACGT GGAGCCTGAC 
CTGCCAGCTG TAGGAGATAC AGGGAATGCA TTAGATAAGA CTGACTGTTT GCAGCGGCGG 
GTAGATGGTG ATTCTGTGGC TTTCAGTGCC CCTTCCTGTG GAAACCAGCG GGAGGAAGCC 
GATATAGAGC TCTGCATAAT CAGTGCCAAG CAGGGGGTTG CTTCACAGCC TGATAAAGGG 
AAGCAAGAAG AGCAAGAGGC TTGCCAGAAG AGGTCAGAGA CTGAAAAGGA CTATAACACT 
GAATCTGGCA ACCTTGGGCT TCAAGACATG GGCCACGCAC AATCTCAGGG TCTCCTCCCC 
ACGGAAACTG GTCAGCCTAC ACAAAGGGAG GTGGAGAGTG AGGGGAGTAC AGATGTCTGT 
TGGCGTGGGG ACTTACCAGA GGAAAGGGAA GGGGACATGA GTAGCAACCA GGAAATAGAC 
TCATATTCTT CAGCACTTTC TTCCCATAAT CTAGAGGGAA ACAGCCAGCA CGATGGTGAG 
ACTGAGCCCC AGTTGCCTAC ACAATGCTCA GAGGGAACAG ATGAAGCTAA ATTGACTTTA 
ATTCAGGTAA ATTCTGAAGT TACTTTGTCT ATACGAAGCG AGGATACCGT TATTTCTGAC 
AGTGTTGACA AGCCACATGG TCTCTTAGAG ACGGAGACAG GAAATCTCAG CGAACACACC 
AAAGGCAACA CTGAGTCAGC TGCTGATTTG GTTGCGGATG ACGCTAGTGT AAAGCAGAAC 
TCTGGATTTA GCGTTTTAGA GGGGGATCTT TTGGAATCAT TAAGTCTACG GCCAAACATG 
GCACCGGGAC CAGTGCATAA GGTTCAGCAA GGTCTGTCAC CAGTTCCTGA GACTTCCTCT 
CATGTAGGTC CAGCTTTGGA CCCCTTTTCT GAGACTACAG AAAGTTTTCA ACAGCCGAAT 
CAATCCACAC AGCTTTTAGA AACTGCATTA GAATGTGAGG ATGTTTCATT AAATAACTCA 
ACCGGTGACA TTAATGCAAT AGCAGGGGTT TCTGTTGACA TTTCTGCAGA GGTTTCACTT 
TGCCAGAATG AAGGGTGTCC TCCTGATGGA AGCAAAGAAG AGGTAGCCCC CAGTGAGAGG 
ATACCAGACC TTAAAGAGCT TACGGAAGAG CCGGAATCAG TTGAGGACAT TATTGTCTCA 
GATGTCTCAG ATTTGGATGA CAAATCTCAA CCTTTTGAAG AGTCTTCTTT GCCTATCGAC 
TCTGAATGTT TTATAAGAGG TGATTTCCAA AACGATGAGT CTACAGTACA GTCTTCTGAA 
TGTACTGGAG GTGACATAAA TGTAGGAACA CTCTTAGGAG AAGAAGAGGC AACTGAAAAA 
ATAGAACAGC CAGACTCTTC ACTTAAACTG GTTGAGCTAA TTCCTGAGGA ATATACTGGA 
GATGCCACAA ACGAAAATAA AAAAATTGAG CCAGAGGAAC AATCTATTTT ACTGCCAACT 
TTGCAAGGGG AATCAGGATT TAAGGATGTT GCCACAGAGG AACCTGTACA GGAAAGGGAA 
ATTGACTCCA CTTCAGAGGA AACAATCAGC GATCTCCCTG ATCCATCGGA AATCACAAAC 
AATGATTGTG CTGAATCCAA TGCACACTGC CAGCTCTCAG AATCCTCCAA GGAGAGCGCA 
AACACATCCT CAGAACCTGC TGAATGTGTG AATGACCTAG ACTTGTCTTG TGCTTTACCT 
CAAACAGAAA TCGAAGGCAG CAGACCTTCT GAGGGCCCCT TAATTGATGG AGTAACAATA 
TGTGCTGAAA TTCCCATTGA TGACAAAGGT GTTGACATGG CTACCAATAT GAGTAAGACG 
ACCATCTCTG AGGATTGTCT GTCCTCTCCT ATGGACACCA CCAGCTGTTC AGTTCTGGAG 
AGCTCCTCCA TGTCTGACCC ACTCAATGCC TCATCCTCAA CAGCTGAGCT CACTTCCCCT 
TTGGATATTA ATAGTGGATT GCATCAGGAT TTGGAACACA CTTCCCCAGT GGATATAGAC 
AATGGTTTGC CTGAGGAACC GCCGCCGTCA GACACACCTG ATTGCCTGAA TGGATTACAT 
CAAGATTCTC CTGATAGTTT GATGGAGCAC AGTGGAACTC CTCAGCCATG TTCTGAGGAA 
CAGTGTGTGG ATCAAATCAC AGATGACAGC ACTTCAGCAG AGGATGCAGC GGACGGACAC 
CAAGACTCCT CAGTAGACAT AGCAAATTCC GAAGCGATCC ACAGAGAGAC CGAAACATTT 
TATTCAGTTG ATGCCAGTCA AGACTCTGTA CTCCAGGAGA AGAAATCTTC TTCAGTGAGC 
TCAGAAATCC CCTCTTTGAC CCCCTCACCT GAGAGCATAC GTCACAAAGA GTCGCCAAGC 
ACTATGCGAG GGTCCGCCAG CGATGGAGAC TCTCTGTTTA GTCAGGATCT GGGCGAGGAC 
AGCGTTTTTA ATAATAAGGC TGAGGACAGC ATTACCATCA CCAGTGGCGT GTCCATGTCC 
TATTCGTCCT CCACAGATGA CTCTTCCAGC TTGGGAACCA CTTGCGCCAC CCCTCAGACT 
GGCACAGAGG CTCCTGCATC AGACCCCTTT CAGGAACAGC CCACATCTAC TGGAGCTTCT 
GGGGAAACAG AGGAGGAAGA GAAAAAGGAC CGTCTCACTG AGGTTCCGGA GCGATCGGCC 
ATTTTACGGA CCACCATACG ATCACTGTCC CCTTTTCGAA GACACAGTTG GGGGCCGGGC 
AAAAATTTGG CAGGGGAGAC AGAGATGAGT CAGCGCAGTG CGGCACAAGG TGAAGTGGAG 
CGCAGATCTG TTGGTCATAG GAGAAGCATG AGCTGGTGTC CTTCTGTTGT CCTGCGCTCT 
GAAAATGATG AAATGAATGA AAGAAGTTAC AGTCTGGAAG GACTTGCACC AGAGAAGGAG 
GGAAGGAAAA GTCTGGTTCA GCAGCGTGTC GGAGAGTCCA GCCAGGAGCC CAAATGTCCA 
TCAAAGGTGG AAAAAGAAGA CAGAGGCTCG CTTGTGTCTC TGACTGAAGA AGAGCAGGAG 
TCAGATCTAG GCGACTGCAG CAGTTTGGGC AGTCAGAAGT CCATCCAGTC GGGTCGGAGA 
TGTGTCCCAC ATTCACAACC CATCCTCACC AAATCTGTGT CCATGGTGGC CATCAGTCAG 
AAGGACCTGG ACGCAATGGG ACGGCTGCGA CCCAAGCGAC GAATCTCCTT CTCCTTCAGC 
ATCTCGCCGA TGCTCCCCAA ATCTAAAACT CTCTTTTCTC TTGGCTCTTC ATCCAGTGAT 
GAGGAGGAGG CAGTCAGATT GAGTTCATTC ACTGGAACAT CAGGATCATT AGAGTACAGT 
ATATCAGAGG AGGATCCTGG GCCATTACGG AGTGACAGTG AAGGCAAAAT TGGAGGAACC 
AAAGTGAGCA GGACATTCAG TTACCTCAAG AGCAAGATGA GCAAGAAGAA CAAGCAGGAG 
AAGGAGAAAG AGAAGAATAA GGAGCGTGAG CGAGATGCCA AAGACAAGGA GAGAAGAGCC 
TCAAACGGAC ATCTCTTCAC TACGGTCATG ACTGTACCCA CAATCCCCTG CCACCAGTGC 
AACAAACTCA TCAACACTAA AGACTCTATC CTCTGCACCA ATTGCAATGC ACAAGTCCAC 
AAAGGCTGCA AAGAGAGTCT TCCTGTCTGC GCTAAAGTCA AGATGAAGCA GCAAAAACAG 
CAGCAGACTG TTACAGATTC TGCCTCGACG CCTAGTGTCA CCCTCAGAAG TAAAACTCTT 
CCAGCAAGGG ATCGTCCGTG GTCCACAATA TCCATTCCCG ATGACCAGCC GGCTCCCCTC 
GCTCCTCCTC GGAAAAGCCC CAGCAGCATC ATGTCTTTCA ACAGCAACCC ACTGTCCAAG 
AGCATGTCCA TTAATAACAT AGCAGGACAA ATGGACGACA CTCCTCTTAA GAAGTTTCTG 
TCCCAGTCAA CGGACTCTCT TCACAAGACC AGTAAAGTCA CGGAGTCTAC AGAATCTCTT 
ACTGATGAGG GTACAGAGAT GATGGACAGC CAGCTGATGG GAGAATTTGA AGCTGACGCC 
AAAGAGCTGG AGGCTGATTC TTATAGCTTC ACGGTAGACA AGAAATTTCT TAAGCAGCTT 
AAAAAGGATG TTATCAAAAG GCAAGACGTC ATCTATGAGC TCATCCAGAC AGAAATGCAC 
CATGTAAGGA CCTTAAAGAT CATGGCTGAT GTTTACAGCA AAGGACTACT GAGAGAAGTT 
CAACTAGAAG CTCAGACGGT GGAGAAGATG TTCCCCATGT TGGACGATGT TCTGGATTTG 
CACACACAGT TCTTTTCACA CCTGCTTGAA AGAAAGAAGG AATCAGGGAG CCAAGAAGAG 
GGCGGCTTTG TCATCCAAAG GATTGGCGAT GTGCTGGTTT CTCAGTTCTC AGGTTCCAGT 
GCTGAGAGAA TGAAGAAGGT CTATGGGAAG TTCTGCGGCA GGCACAATGA AGCTGTGAAC 
TTCTACAAGG AGCTTCACAC CAAAGACAAA CGCTTTCAAG CTTTCATCAA GAAAAAGATG 
AGCAGCCCTG TTGTCCGACG GCTAAGCATT CCTGAGTGTA TATTGTTAGT CACACAACGA 
ATCACAAAAT ATCCCGTGTT ACTACAGAGA ATACTGCAGC ACACGAAAGA AAACGAGGAG 
GACCATGCAG ACGTTAGTCA AGGTCTGAAG CTGGTGAAGG AGGTGATTGC TGCCGTGGAC 
AATAAAGTGA ATGAGCATGA GAAGAAGAAG AGGCTAAAGG AAGTGTACAG CCGGACGGAC 
AACAAGTCCA TCATGAGGAT GAAGAGCGGG CAGATGTTTG CCAGAGAGGA TCTGTTACGT 
GGGCGGAAGT TAATTCACGA CGGGCCGCTG CAGCTCAAGA ACTCAGCTGG CAGACTCAAG 
GATGTCCAGG CTCTGCTGCT GAGGGACGTG ATTGTGTTCC TGCAGGAGAA AGATCAGAAG 
TACATTTTCG CCTCTTTGGA CCAGCGCTCC ACCGTCATCT CCCTGCAGAA GCTCATTGTG 
AGGGAAGTAG CCAATGAGGA GAAAGCTCTG TTTCTGATCA CAGCAGGCAT CGAGAAGCCC 
GAGATGGTAG AAGTGTACAC AAGCTCCAAA GAGGAGAGAA ACACATGGAT GCAGCTCATT 
CAGGAGGCCA TGCAGTCTAT ACTGTCCATT TCCTCCAGGG AGAGAGATGA TGATGAAGGT 
GTGCCAAGTG AAAACGAAGA GGACAGGAGA CTTCAGGAAA TCAAGGTCAA GGAGTTCAGA 
GATCAGCTGC ACAAAAGAGA CGAGCAGATC TTGACGTTAC TGGAGGAGAA GGTGAAGTTG 
TTCAGGGACA TGTGTGACTG TGGTGCACCG GACGAGACGG GGCTTCGTAA TCGAATGCTC 
TTCAGGGCCA CGACGGATGA TGTCACCAGA GGAGAGCCAA TCATGAAGGA GGCTCTGAAG 
GAAGTTGAAA CGCTGCAGGT TCTGGTTAAT GACAGTCTTG GCGGTGCGGT TCAGGAAGGT 
GTGTGCGCGG CTGGACATGT GACTTTGCCA CGACGAGCGG AAACATTTGG AGGTTTCGAC 
AGTCACCAGA TGAACATCTC TAAGAGCAAA GATGGAGACA GAGAGGAGCT GGAGGAGTCT 
GCAGAGCTCC GCAGGACTGA GTCAGACAGT GTGTTGAAGA AGACGAGTAA GACCAACCCT 
TTACTGCTGG TCAGGAGAAA CAATGAGCAG GTTCTTCAGA GCGTTGCTCA TCTTCATGAC 
CTGCTGAACA CATTACAAGC TGTGGTGGTC CAGCAGGACA CCTTCATCGA AGACCAGCGT 
CACGCTCATC CCTCCTCACG CCACCCATCC ATCTCCTCCT CTACCTCCTC TTTATCATCC 
ACCTCATCCC GGCCGAGCTC TCTAATCGAG CAGGAGAAAC AGCGCAGCCT GGAGAAGCAG 
CGGCAGGAGG TGGCCAGCCT CCAAAAACAG CAGGCGGCCC ACGCCGAGGA GAGGCGGCGC 
AGGGAAAAGC AGTGGGAGAC CCGAGAGAAA GAGCTAACCC AGAGGGAGAT GCAGATCCAG 
AGCCAAGAAG AGGAGGCGCA GAGGAGAGCC AGACAGCTGG AGGAGGAGAA ACAGGAGCTG 
CAGAGTAAGA AAGAGGAGTA TCAGAGAGAC CTGGCACGAC TGCGGGACAG CCAGAAGAGA 
CTGGAGAGAG AGAGAGAGCA GGTGCAGAGA GAGGCAGATG AGCTTAAACT GACTGAGGAA 
GTGACAAAGA ACCGGTCTCC GTCAACCACG TCTGAAGACT CCACGAAGTT CCAGAGCACC 
GGTTCATTAG ACCGTGACCC ATCAGAGATG GAGCTCTCCT CCTCCCCGTC AGCCAGAGAC 
TTCCTGTCCC GCATGGACTC CAAACGCAAA GGGAAAAACC TCAATCCATT CTCATCCAAC 
TCAAGCCAGA AGAGCCAGAA CAGCGAGGCT CAGAGCCAGA TGCCCAGCCG TCTGCTCCAG 
CTGGCCAAAC CCAAGGAGAA GAAGGACAAG AAGAAGAAGA AGGGGAAAGG CCAGCAGAGC 
CAGACTGCAG ACTCTCAGAG CACTGTTGTT CCAGGCGCTA CCCCAGACGG AGAGATCTTT 
TTCTGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

wu:fi15d04 ( XM_009297835.2 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_009296110.1 Danio rerio wu:fi15d04 (wu:fi15d04), transcript variant X5, mRNA.

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CloneID	ODa39929
Clone ID Related Accession (Same CDS sequence)	XM_009297835.2
Accession Version	XM_009297835.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7689bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-23
Organism	Danio rerio(Zebrafish)
Product	A-kinase anchor protein 13 isoform X5
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007136.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_009297835.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAGGGTG TTCCACAAGT GGCCAGTGCC ATGAAACTGA ATCCACAGCA AGCCCCATTA 
TATGGAGAGT GTCTGCTGAC GGTCCAGCTC TGTGAGGATG AGTGTGTTGA GGATGAGGAT 
GATGTGGAGT TTTACCTGCT CTTTGCTGGA TCCACACAGA GACATGTGTC CAGCACACTG 
AAGATCAGCC ATGTCACGCT GCAGGCCGTC TGTCCAGCTC ATGATCGCAG TGAAACGGTA 
CGGGTCACAC TATGCCAGGC TCGTCCCGGC GGCTCAGTGG ACCCAGTGTC CGAGGAGTGT 
TTCCAGTTTG TCCAGGACCT GGCTCTGGAC ATGGCCCAGT TCCTGGTAAA TGCGGCGGGG 
AAAACAGAAG GAGCGCTGCT GCTGGACGAG TGTCAGATCC CACTGAAAGA GTGCGAGCGG 
CTGGACGATA CTCTTGCTCT GGCTCTCCGG CATCTGCCTT TGCCTGCTGG ATGGAGCGTT 
TTGGGCACAG ATTTGGACAC GGGCACTTGC ACAGGCTCGG GCATCGCAGA GCCTGGACCA 
CACGATACAC TTTTGCACTT CGCTGCGAAG CGCGGACTAC GCAAGGTTGC ACTCTTTCTG 
CTCCAGCAAC CAGGGGGAAG AGAGGCTCTC CGGCTTCCCA ACAAACAGGG TCGCACACCG 
GCCAAGGTCG CCCAGAAGAG GGGACACTCT CAGCTCCACA AACTGCTCAC GGAGGTTGAC 
AATCTGCCCG AGCTGGAGAC GAAAACATCT AAGTGGTGTT ACCCAGAAGG CAGAGTCCTG 
CAACACCACC CCAAGCTAAA CACTTACACC CTTACCCTGG AGGATGTGCC CAGGAGCTCC 
CCACCAAGCC TTCAGTGTGA TGTGGAGGAG CTCCAGCGTC TCATTCAGTC ACATCAGAAG 
GAAGATCCTC CAACACCGCA TAAGTCAGCA CTGCTGATTC TCAATAGAGA CTCCAGTAAT 
AGCACAGAGA CAGATGACTG CCTTCCGCCC AACTCAGTGG AACCTGAGCA TCAGGAGCAC 
AACAAACAAG AAGAAACTTG CAAATCGCTT TTAAACAGCC TTACCTTTGA GGATGGATTG 
TGTGTTCATC AAAACGGCAC AGGAGATCAT GTCCAGTTGC TGGATGGGGA CGTGGAGCCT 
GACCTGCCAG CTGTAGGAGA TACAGGGAAT GCATTAGATA AGACTGACTG TTTGCAGCGG 
CGGGTAGATG GTGATTCTGT GGCTTTCAGT GCCCCTTCCT GTGGAAACCA GCGGGAGGAA 
GCCGATATAG AGCTCTGCAT AATCAGTGCC AAGCAGGGGG TTGCTTCACA GCCTGATAAA 
GGGAAGCAAG AAGAGCAAGA GGCTTGCCAG AAGAGGTCAG AGACTGAAAA GGACTATAAC 
ACTGAATCTG GCAACCTTGG GCTTCAAGAC ATGGGCCACG CACAATCTCA GGGTCTCCTC 
CCCACGGAAA CTGGTCAGCC TACACAAAGG GAGGTGGAGA GTGAGGGGAG TACAGATGTC 
TGTTGGCGTG GGGACTTACC AGAGGAAAGG GAAGGGGACA TGAGTAGCAA CCAGGAAATA 
GACTCATATT CTTCAGCACT TTCTTCCCAT AATCTAGAGG GAAACAGCCA GCACGATGGT 
GAGACTGAGC CCCAGTTGCC TACACAATGC TCAGAGGGAA CAGATGAAGC TAAATTGACT 
TTAATTCAGG TAAATTCTGA AGTTACTTTG TCTATACGAA GCGAGGATAC CGTTATTTCT 
GACAGTGTTG ACAAGCCACA TGGTCTCTTA GAGACGGAGA CAGGAAATCT CAGCGAACAC 
ACCAAAGGCA ACACTGAGTC AGCTGCTGAT TTGGTTGCGG ATGACGCTAG TGTAAAGCAG 
AACTCTGGAT TTAGCGTTTT AGAGGGGGAT CTTTTGGAAT CATTAAGTCT ACGGCCAAAC 
ATGGCACCGG GACCAGTGCA TAAGGTTCAG CAAGGTCTGT CACCAGTTCC TGAGACTTCC 
TCTCATGTAG GTCCAGCTTT GGACCCCTTT TCTGAGACTA CAGAAAGTTT TCAACAGCCG 
AATCAATCCA CACAGCTTTT AGAAACTGCA TTAGAATGTG AGGATGTTTC ATTAAATAAC 
TCAACCGGTG ACATTAATGC AATAGCAGGG GTTTCTGTTG ACATTTCTGC AGAGGTTTCA 
CTTTGCCAGA ATGAAGGGTG TCCTCCTGAT GGAAGCAAAG AAGAGGTAGC CCCCAGTGAG 
AGGATACCAG ACCTTAAAGA GCTTACGGAA GAGCCGGAAT CAGTTGAGGA CATTATTGTC 
TCAGATGTCT CAGATTTGGA TGACAAATCT CAACCTTTTG AAGAGTCTTC TTTGCCTATC 
GACTCTGAAT GTTTTATAAG AGGTGATTTC CAAAACGATG AGTCTACAGT ACAGTCTTCT 
GAATGTACTG GAGGTGACAT AAATGTAGGA ACACTCTTAG GAGAAGAAGA GGCAACTGAA 
AAAATAGAAC AGCCAGACTC TTCACTTAAA CTGGTTGAGC TAATTCCTGA GGAATATACT 
GGAGATGCCA CAAACGAAAA TAAAAAAATT GAGCCAGAGG AACAATCTAT TTTACTGCCA 
ACTTTGCAAG GGGAATCAGG ATTTAAGGAT GTTGCCACAG AGGAACCTGT ACAGGAAAGG 
GAAATTGACT CCACTTCAGA GGAAACAATC AGCGATCTCC CTGATCCATC GGAAATCACA 
AACAATGATT GTGCTGAATC CAATGCACAC TGCCAGCTCT CAGAATCCTC CAAGGAGAGC 
GCAAACACAT CCTCAGAACC TGCTGAATGT GTGAATGACC TAGACTTGTC TTGTGCTTTA 
CCTCAAACAG AAATCGAAGG CAGCAGACCT TCTGAGGGCC CCTTAATTGA TGGAGTAACA 
ATATGTGCTG AAATTCCCAT TGATGACAAA GGTGTTGACA TGGCTACCAA TATGAGTAAG 
ACGACCATCT CTGAGGATTG TCTGTCCTCT CCTATGGACA CCACCAGCTG TTCAGTTCTG 
GAGAGCTCCT CCATGTCTGA CCCACTCAAT GCCTCATCCT CAACAGCTGA GCTCACTTCC 
CCTTTGGATA TTAATAGTGG ATTGCATCAG GATTTGGAAC ACACTTCCCC AGTGGATATA 
GACAATGGTT TGCCTGAGGA ACCGCCGCCG TCAGACACAC CTGATTGCCT GAATGGATTA 
CATCAAGATT CTCCTGATAG TTTGATGGAG CACAGTGGAA CTCCTCAGCC ATGTTCTGAG 
GAACAGTGTG TGGATCAAAT CACAGATGAC AGCACTTCAG CAGAGGATGC AGCGGACGGA 
CACCAAGACT CCTCAGTAGA CATAGCAAAT TCCGAAGCGA TCCACAGAGA GACCGAAACA 
TTTTATTCAG TTGATGCCAG TCAAGACTCT GTACTCCAGG AGAAGAAATC TTCTTCAGTG 
AGCTCAGAAA TCCCCTCTTT GACCCCCTCA CCTGAGAGCA TACGTCACAA AGAGTCGCCA 
AGCACTATGC GAGGGTCCGC CAGCGATGGA GACTCTCTGT TTAGTCAGGA TCTGGGCGAG 
GACAGCGTTT TTAATAATAA GGCTGAGGAC AGCATTACCA TCACCAGTGG CGTGTCCATG 
TCCTATTCGT CCTCCACAGA TGACTCTTCC AGCTTGGGAA CCACTTGCGC CACCCCTCAG 
ACTGGCACAG AGGCTCCTGC ATCAGACCCC TTTCAGGAAC AGCCCACATC TACTGGAGCT 
TCTGGGGAAA CAGAGGAGGA AGAGAAAAAG GACCGTCTCA CTGAGGTTCC GGAGCGATCG 
GCCATTTTAC GGACCACCAT ACGATCACTG TCCCCTTTTC GAAGACACAG TTGGGGGCCG 
GGCAAAAATT TGGCAGGGGA GACAGAGATG AGTCAGCGCA GTGCGGCACA AGGTGAAGTG 
GAGCGCAGAT CTGTTGGTCA TAGGAGAAGC ATGAGCTGGT GTCCTTCTGT TGTCCTGCGC 
TCTGAAAATG ATGAAATGAA TGAAAGAAGT TACAGTCTGG AAGGACTTGC ACCAGAGAAG 
GAGGGAAGGA AAAGTCTGGT TCAGCAGCGT GTCGGAGAGT CCAGCCAGGA GCCCAAATGT 
CCATCAAAGG TGGAAAAAGA AGACAGAGGC TCGCTTGTGT CTCTGACTGA AGAAGAGCAG 
GAGTCAGATC TAGGCGACTG CAGCAGTTTG GGCAGTCAGA AGTCCATCCA GTCGGGTCGG 
AGATGTGTCC CACATTCACA ACCCATCCTC ACCAAATCTG TGTCCATGGT GGCCATCAGT 
CAGAAGGACC TGGACGCAAT GGGACGGCTG CGACCCAAGC GACGAATCTC CTTCTCCTTC 
AGCATCTCGC CGATGCTCCC CAAATCTAAA ACTCTCTTTT CTCTTGGCTC TTCATCCAGT 
GATGAGGAGG AGGCAGTCAG ATTGAGTTCA TTCACTGGAA CATCAGGATC ATTAGAGTAC 
AGTATATCAG AGGAGGATCC TGGGCCATTA CGGAGTGACA GTGAAGGCAA AATTGGAGGA 
ACCAAAGTGA GCAGGACATT CAGTTACCTC AAGAGCAAGA TGAGCAAGAA GAACAAGGAG 
AAGGAGAAAG AGAAGAATAA GGAGCGTGAG CGAGATGCCA AAGACAAGGA GAGAAGAGCC 
TCAAACGGAC ATCTCTTCAC TACGGTCATG ACTGTACCCA CAATCCCCTG CCACCAGTGC 
AACAAACTCA TCAACACTAA AGACTCTATC CTCTGCACCA ATTGCAATGC ACAAGTCCAC 
AAAGGCTGCA AAGAGAGTCT TCCTGTCTGC GCTAAAGTCA AGATGAAGCA GCAAAAACAG 
CAGCAGACTG TTACAGATTC TGCCTCGACG CCTAGTGTCA CCCTCAGAAG TAAAACTCTT 
CCAGCAAGGG ATCGTCCGTG GTCCACAATA TCCATTCCCG ATGACCAGCC GGCTCCCCTC 
GCTCCTCCTC GGAAAAGCCC CAGCAGCATC ATGTCTTTCA ACAGCAACCC ACTGTCCAAG 
AGCATGTCCA TTAATAACAT AGCAGGACAA ATGGACGACA CTCCTCTTAA GAAGTTTCTG 
TCCCAGTCAA CGGACTCTCT TCACAAGACC AGTAAAGTCA CGGAGTCTAC AGAATCTCTT 
ACTGATGAGG GTACAGAGAT GATGGACAGC CAGCTGATGG GAGAATTTGA AGCTGACGCC 
AAAGAGCTGG AGGCTGATTC TTATAGCTTC ACGGTAGACA AGAAATTTCT TAAGCAGCTT 
AAAAAGGATG TTATCAAAAG GCAAGACGTC ATCTATGAGC TCATCCAGAC AGAAATGCAC 
CATGTAAGGA CCTTAAAGAT CATGGCTGAT GTTTACAGCA AAGGACTACT GAGAGAAGTT 
CAACTAGAAG CTCAGACGGT GGAGAAGATG TTCCCCATGT TGGACGATGT TCTGGATTTG 
CACACACAGT TCTTTTCACA CCTGCTTGAA AGAAAGAAGG AATCAGGGAG CCAAGAAGAG 
GGCGGCTTTG TCATCCAAAG GATTGGCGAT GTGCTGGTTT CTCAGTTCTC AGGTTCCAGT 
GCTGAGAGAA TGAAGAAGGT CTATGGGAAG TTCTGCGGCA GGCACAATGA AGCTGTGAAC 
TTCTACAAGG AGCTTCACAC CAAAGACAAA CGCTTTCAAG CTTTCATCAA GAAAAAGATG 
AGCAGCCCTG TTGTCCGACG GCTAAGCATT CCTGAGTGTA TATTGTTAGT CACACAACGA 
ATCACAAAAT ATCCCGTGTT ACTACAGAGA ATACTGCAGC ACACGAAAGA AAACGAGGAG 
GACCATGCAG ACGTTAGTCA AGGTCTGAAG CTGGTGAAGG AGGTGATTGC TGCCGTGGAC 
AATAAAGTGA ATGAGCATGA GAAGAAGAAG AGGCTAAAGG AAGTGTACAG CCGGACGGAC 
AACAAGTCCA TCATGAGGAT GAAGAGCGGG CAGATGTTTG CCAGAGAGGA TCTGTTACGT 
GGGCGGAAGT TAATTCACGA CGGGCCGCTG CAGCTCAAGA ACTCAGCTGG CAGACTCAAG 
GATGTCCAGG CTCTGCTGCT GAGGGACGTG ATTGTGTTCC TGCAGGAGAA AGATCAGAAG 
TACATTTTCG CCTCTTTGGA CCAGCGCTCC ACCGTCATCT CCCTGCAGAA GCTCATTGTG 
AGGGAAGTAG CCAATGAGGA GAAAGCTCTG TTTCTGATCA CAGCAGGCAT CGAGAAGCCC 
GAGATGGTAG AAGTGTACAC AAGCTCCAAA GAGGAGAGAA ACACATGGAT GCAGCTCATT 
CAGGAGGCCA TGCAGTCTAT ACTGTCCATT TCCTCCAGGG AGAGAGATGA TGATGAAGGT 
GTGCCAAGTG AAAACGAAGA GGACAGGAGA CTTCAGGAAA TCAAGGTCAA GGAGTTCAGA 
GATCAGCTGC ACAAAAGAGA CGAGCAGATC TTGACGTTAC TGGAGGAGAA GGTGAAGTTG 
TTCAGGGACA TGTGTGACTG TGGTGCACCG GACGAGACGG GGCTTCGTAA TCGAATGCTC 
TTCAGGGCCA CGACGGATGA TGTCACCAGA GGAGAGCCAA TCATGAAGGA GGCTCTGAAG 
GAAGTTGAAA CGCTGCAGGT TCTGGTTAAT GACAGTCTTG GCGGTGCGGT TCAGGAAGGT 
GTGTGCGCGG CTGGACATGT GACTTTGCCA CGACGAGCGG AAACATTTGG AGGTTTCGAC 
AGTCACCAGA TGAACATCTC TAAGAGCAAA GATGGAGACA GAGAGGAGCT GGAGGAGTCT 
GCAGAGCTCC GCAGGACTGA GTCAGACAGT GTGTTGAAGA AGACGAGTAA GACCAACCCT 
TTACTGCTGG TCAGGAGAAA CAATGAGCAG GTTCTTCAGA GCGTTGCTCA TCTTCATGAC 
CTGCTGAACA CATTACAAGC TGTGGTGGTC CAGCAGGACA CCTTCATCGA AGACCAGCGT 
CACGCTCATC CCTCCTCACG CCACCCATCC ATCTCCTCCT CTACCTCCTC TTTATCATCC 
ACCTCATCCC GGCCGAGCTC TCTAATCGAG CAGGAGAAAC AGCGCAGCCT GGAGAAGCAG 
CGGCAGGAGG TGGCCAGCCT CCAAAAACAG CAGGCGGCCC ACGCCGAGGA GAGGCGGCGC 
AGGGAAAAGC AGTGGGAGAC CCGAGAGAAA GAGCTAACCC AGAGGGAGAT GCAGATCCAG 
AGCCAAGAAG AGGAGGCGCA GAGGAGAGCC AGACAGCTGG AGGAGGAGAA ACAGGAGCTG 
CAGAGTAAGA AAGAGGAGTA TCAGAGAGAC CTGGCACGAC TGCGGGACAG CCAGAAGAGA 
CTGGAGAGAG AGAGAGAGCA GGTGCAGAGA GAGGCAGATG AGCTTAAACT GACTGAGGAA 
GTGACAAAGA ACCGGTCTCC GTCAACCACG TCTGAAGACT CCACGAAGTT CCAGAGCACC 
GGTTCATTAG ACCGTGACCC ATCAGAGATG GAGCTCTCCT CCTCCCCGTC AGCCAGAGAC 
TTCCTGTCCC GCATGGACTC CAAACGCAAA GGGAAAAACC TCAATCCATT CTCATCCAAC 
TCAAGCCAGA AGAGCCAGAA CAGCGAGGCT CAGAGCCAGA TGCCCAGCCG TCTGCTCCAG 
CTGGCCAAAC CCAAGGAGAA GAAGGACAAG AAGAAGAAGA AGGGGAAAGG CCAGCAGAGC 
CAGACTGCAG ACTCTCAGAG CACTGTTGTT CCAGGCGCTA CCCCAGACGG AGAGATCTTT 
TTCTGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_009296110.1
CDS	28..7716
Translation	MKGVPQVASAMKLNPQQAPLYGECLLTVQLCEDECVEDEDDVEFYLLFAGSTQRHVSSTLKISHVTLQAVCPAHDRSETVRVTLCQARPGGSVDPVSEECFQFVQDLALDMAQFLVNAAGKTEGALLLDECQIPLKECERLDDTLALALRHLPLPAGWSVLGTDLDTGTCTGSGIAEPGPHDTLLHFAAKRGLRKVALFLLQQPGGREALRLPNKQGRTPAKVAQKRGHSQLHKLLTEVDNLPELETKTSKWCYPEGRVLQHHPKLNTYTLTLEDVPRSSPPSLQCDVEELQRLIQSHQKEDPPTPHKSALLILNRDSSNSTETDDCLPPNSVEPEHQEHNKQEETCKSLLNSLTFEDGLCVHQNGTGDHVQLLDGDVEPDLPAVGDTGNALDKTDCLQRRVDGDSVAFSAPSCGNQREEADIELCIISAKQGVASQPDKGKQEEQEACQKRSETEKDYNTESGNLGLQDMGHAQSQGLLPTETGQPTQREVESEGSTDVCWRGDLPEEREGDMSSNQEIDSYSSALSSHNLEGNSQHDGETEPQLPTQCSEGTDEAKLTLIQVNSEVTLSIRSEDTVISDSVDKPHGLLETETGNLSEHTKGNTESAADLVADDASVKQNSGFSVLEGDLLESLSLRPNMAPGPVHKVQQGLSPVPETSSHVGPALDPFSETTESFQQPNQSTQLLETALECEDVSLNNSTGDINAIAGVSVDISAEVSLCQNEGCPPDGSKEEVAPSERIPDLKELTEEPESVEDIIVSDVSDLDDKSQPFEESSLPIDSECFIRGDFQNDESTVQSSECTGGDINVGTLLGEEEATEKIEQPDSSLKLVELIPEEYTGDATNENKKIEPEEQSILLPTLQGESGFKDVATEEPVQEREIDSTSEETISDLPDPSEITNNDCAESNAHCQLSESSKESANTSSEPAECVNDLDLSCALPQTEIEGSRPSEGPLIDGVTICAEIPIDDKGVDMATNMSKTTISEDCLSSPMDTTSCSVLESSSMSDPLNASSSTAELTSPLDINSGLHQDLEHTSPVDIDNGLPEEPPPSDTPDCLNGLHQDSPDSLMEHSGTPQPCSEEQCVDQITDDSTSAEDAADGHQDSSVDIANSEAIHRETETFYSVDASQDSVLQEKKSSSVSSEIPSLTPSPESIRHKESPSTMRGSASDGDSLFSQDLGEDSVFNNKAEDSITITSGVSMSYSSSTDDSSSLGTTCATPQTGTEAPASDPFQEQPTSTGASGETEEEEKKDRLTEVPERSAILRTTIRSLSPFRRHSWGPGKNLAGETEMSQRSAAQGEVERRSVGHRRSMSWCPSVVLRSENDEMNERSYSLEGLAPEKEGRKSLVQQRVGESSQEPKCPSKVEKEDRGSLVSLTEEEQESDLGDCSSLGSQKSIQSGRRCVPHSQPILTKSVSMVAISQKDLDAMGRLRPKRRISFSFSISPMLPKSKTLFSLGSSSSDEEEAVRLSSFTGTSGSLEYSISEEDPGPLRSDSEGKIGGTKVSRTFSYLKSKMSKKNKEKEKEKNKERERDAKDKERRASNGHLFTTVMTVPTIPCHQCNKLINTKDSILCTNCNAQVHKGCKESLPVCAKVKMKQQKQQQTVTDSASTPSVTLRSKTLPARDRPWSTISIPDDQPAPLAPPRKSPSSIMSFNSNPLSKSMSINNIAGQMDDTPLKKFLSQSTDSLHKTSKVTESTESLTDEGTEMMDSQLMGEFEADAKELEADSYSFTVDKKFLKQLKKDVIKRQDVIYELIQTEMHHVRTLKIMADVYSKGLLREVQLEAQTVEKMFPMLDDVLDLHTQFFSHLLERKKESGSQEEGGFVIQRIGDVLVSQFSGSSAERMKKVYGKFCGRHNEAVNFYKELHTKDKRFQAFIKKKMSSPVVRRLSIPECILLVTQRITKYPVLLQRILQHTKENEEDHADVSQGLKLVKEVIAAVDNKVNEHEKKKRLKEVYSRTDNKSIMRMKSGQMFAREDLLRGRKLIHDGPLQLKNSAGRLKDVQALLLRDVIVFLQEKDQKYIFASLDQRSTVISLQKLIVREVANEEKALFLITAGIEKPEMVEVYTSSKEERNTWMQLIQEAMQSILSISSRERDDDEGVPSENEEDRRLQEIKVKEFRDQLHKRDEQILTLLEEKVKLFRDMCDCGAPDETGLRNRMLFRATTDDVTRGEPIMKEALKEVETLQVLVNDSLGGAVQEGVCAAGHVTLPRRAETFGGFDSHQMNISKSKDGDREELEESAELRRTESDSVLKKTSKTNPLLLVRRNNEQVLQSVAHLHDLLNTLQAVVVQQDTFIEDQRHAHPSSRHPSISSSTSSLSSTSSRPSSLIEQEKQRSLEKQRQEVASLQKQQAAHAEERRRREKQWETREKELTQREMQIQSQEEEAQRRARQLEEEKQELQSKKEEYQRDLARLRDSQKRLEREREQVQREADELKLTEEVTKNRSPSTTSEDSTKFQSTGSLDRDPSEMELSSSPSARDFLSRMDSKRKGKNLNPFSSNSSQKSQNSEAQSQMPSRLLQLAKPKEKKDKKKKKGKGQQSQTADSQSTVVPGATPDGEIFFC

Target ORF information:

RefSeq Version	XM_009297835.2
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio wu:fi15d04 (wu:fi15d04), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_009297835.2

ORF Insert Sequence:

ATGAAGGGTG TTCCACAAGT GGCCAGTGCC ATGAAACTGA ATCCACAGCA AGCCCCATTA 
TATGGAGAGT GTCTGCTGAC GGTCCAGCTC TGTGAGGATG AGTGTGTTGA GGATGAGGAT 
GATGTGGAGT TTTACCTGCT CTTTGCTGGA TCCACACAGA GACATGTGTC CAGCACACTG 
AAGATCAGCC ATGTCACGCT GCAGGCCGTC TGTCCAGCTC ATGATCGCAG TGAAACGGTA 
CGGGTCACAC TATGCCAGGC TCGTCCCGGC GGCTCAGTGG ACCCAGTGTC CGAGGAGTGT 
TTCCAGTTTG TCCAGGACCT GGCTCTGGAC ATGGCCCAGT TCCTGGTAAA TGCGGCGGGG 
AAAACAGAAG GAGCGCTGCT GCTGGACGAG TGTCAGATCC CACTGAAAGA GTGCGAGCGG 
CTGGACGATA CTCTTGCTCT GGCTCTCCGG CATCTGCCTT TGCCTGCTGG ATGGAGCGTT 
TTGGGCACAG ATTTGGACAC GGGCACTTGC ACAGGCTCGG GCATCGCAGA GCCTGGACCA 
CACGATACAC TTTTGCACTT CGCTGCGAAG CGCGGACTAC GCAAGGTTGC ACTCTTTCTG 
CTCCAGCAAC CAGGGGGAAG AGAGGCTCTC CGGCTTCCCA ACAAACAGGG TCGCACACCG 
GCCAAGGTCG CCCAGAAGAG GGGACACTCT CAGCTCCACA AACTGCTCAC GGAGGTTGAC 
AATCTGCCCG AGCTGGAGAC GAAAACATCT AAGTGGTGTT ACCCAGAAGG CAGAGTCCTG 
CAACACCACC CCAAGCTAAA CACTTACACC CTTACCCTGG AGGATGTGCC CAGGAGCTCC 
CCACCAAGCC TTCAGTGTGA TGTGGAGGAG CTCCAGCGTC TCATTCAGTC ACATCAGAAG 
GAAGATCCTC CAACACCGCA TAAGTCAGCA CTGCTGATTC TCAATAGAGA CTCCAGTAAT 
AGCACAGAGA CAGATGACTG CCTTCCGCCC AACTCAGTGG AACCTGAGCA TCAGGAGCAC 
AACAAACAAG AAGAAACTTG CAAATCGCTT TTAAACAGCC TTACCTTTGA GGATGGATTG 
TGTGTTCATC AAAACGGCAC AGGAGATCAT GTCCAGTTGC TGGATGGGGA CGTGGAGCCT 
GACCTGCCAG CTGTAGGAGA TACAGGGAAT GCATTAGATA AGACTGACTG TTTGCAGCGG 
CGGGTAGATG GTGATTCTGT GGCTTTCAGT GCCCCTTCCT GTGGAAACCA GCGGGAGGAA 
GCCGATATAG AGCTCTGCAT AATCAGTGCC AAGCAGGGGG TTGCTTCACA GCCTGATAAA 
GGGAAGCAAG AAGAGCAAGA GGCTTGCCAG AAGAGGTCAG AGACTGAAAA GGACTATAAC 
ACTGAATCTG GCAACCTTGG GCTTCAAGAC ATGGGCCACG CACAATCTCA GGGTCTCCTC 
CCCACGGAAA CTGGTCAGCC TACACAAAGG GAGGTGGAGA GTGAGGGGAG TACAGATGTC 
TGTTGGCGTG GGGACTTACC AGAGGAAAGG GAAGGGGACA TGAGTAGCAA CCAGGAAATA 
GACTCATATT CTTCAGCACT TTCTTCCCAT AATCTAGAGG GAAACAGCCA GCACGATGGT 
GAGACTGAGC CCCAGTTGCC TACACAATGC TCAGAGGGAA CAGATGAAGC TAAATTGACT 
TTAATTCAGG TAAATTCTGA AGTTACTTTG TCTATACGAA GCGAGGATAC CGTTATTTCT 
GACAGTGTTG ACAAGCCACA TGGTCTCTTA GAGACGGAGA CAGGAAATCT CAGCGAACAC 
ACCAAAGGCA ACACTGAGTC AGCTGCTGAT TTGGTTGCGG ATGACGCTAG TGTAAAGCAG 
AACTCTGGAT TTAGCGTTTT AGAGGGGGAT CTTTTGGAAT CATTAAGTCT ACGGCCAAAC 
ATGGCACCGG GACCAGTGCA TAAGGTTCAG CAAGGTCTGT CACCAGTTCC TGAGACTTCC 
TCTCATGTAG GTCCAGCTTT GGACCCCTTT TCTGAGACTA CAGAAAGTTT TCAACAGCCG 
AATCAATCCA CACAGCTTTT AGAAACTGCA TTAGAATGTG AGGATGTTTC ATTAAATAAC 
TCAACCGGTG ACATTAATGC AATAGCAGGG GTTTCTGTTG ACATTTCTGC AGAGGTTTCA 
CTTTGCCAGA ATGAAGGGTG TCCTCCTGAT GGAAGCAAAG AAGAGGTAGC CCCCAGTGAG 
AGGATACCAG ACCTTAAAGA GCTTACGGAA GAGCCGGAAT CAGTTGAGGA CATTATTGTC 
TCAGATGTCT CAGATTTGGA TGACAAATCT CAACCTTTTG AAGAGTCTTC TTTGCCTATC 
GACTCTGAAT GTTTTATAAG AGGTGATTTC CAAAACGATG AGTCTACAGT ACAGTCTTCT 
GAATGTACTG GAGGTGACAT AAATGTAGGA ACACTCTTAG GAGAAGAAGA GGCAACTGAA 
AAAATAGAAC AGCCAGACTC TTCACTTAAA CTGGTTGAGC TAATTCCTGA GGAATATACT 
GGAGATGCCA CAAACGAAAA TAAAAAAATT GAGCCAGAGG AACAATCTAT TTTACTGCCA 
ACTTTGCAAG GGGAATCAGG ATTTAAGGAT GTTGCCACAG AGGAACCTGT ACAGGAAAGG 
GAAATTGACT CCACTTCAGA GGAAACAATC AGCGATCTCC CTGATCCATC GGAAATCACA 
AACAATGATT GTGCTGAATC CAATGCACAC TGCCAGCTCT CAGAATCCTC CAAGGAGAGC 
GCAAACACAT CCTCAGAACC TGCTGAATGT GTGAATGACC TAGACTTGTC TTGTGCTTTA 
CCTCAAACAG AAATCGAAGG CAGCAGACCT TCTGAGGGCC CCTTAATTGA TGGAGTAACA 
ATATGTGCTG AAATTCCCAT TGATGACAAA GGTGTTGACA TGGCTACCAA TATGAGTAAG 
ACGACCATCT CTGAGGATTG TCTGTCCTCT CCTATGGACA CCACCAGCTG TTCAGTTCTG 
GAGAGCTCCT CCATGTCTGA CCCACTCAAT GCCTCATCCT CAACAGCTGA GCTCACTTCC 
CCTTTGGATA TTAATAGTGG ATTGCATCAG GATTTGGAAC ACACTTCCCC AGTGGATATA 
GACAATGGTT TGCCTGAGGA ACCGCCGCCG TCAGACACAC CTGATTGCCT GAATGGATTA 
CATCAAGATT CTCCTGATAG TTTGATGGAG CACAGTGGAA CTCCTCAGCC ATGTTCTGAG 
GAACAGTGTG TGGATCAAAT CACAGATGAC AGCACTTCAG CAGAGGATGC AGCGGACGGA 
CACCAAGACT CCTCAGTAGA CATAGCAAAT TCCGAAGCGA TCCACAGAGA GACCGAAACA 
TTTTATTCAG TTGATGCCAG TCAAGACTCT GTACTCCAGG AGAAGAAATC TTCTTCAGTG 
AGCTCAGAAA TCCCCTCTTT GACCCCCTCA CCTGAGAGCA TACGTCACAA AGAGTCGCCA 
AGCACTATGC GAGGGTCCGC CAGCGATGGA GACTCTCTGT TTAGTCAGGA TCTGGGCGAG 
GACAGCGTTT TTAATAATAA GGCTGAGGAC AGCATTACCA TCACCAGTGG CGTGTCCATG 
TCCTATTCGT CCTCCACAGA TGACTCTTCC AGCTTGGGAA CCACTTGCGC CACCCCTCAG 
ACTGGCACAG AGGCTCCTGC ATCAGACCCC TTTCAGGAAC AGCCCACATC TACTGGAGCT 
TCTGGGGAAA CAGAGGAGGA AGAGAAAAAG GACCGTCTCA CTGAGGTTCC GGAGCGATCG 
GCCATTTTAC GGACCACCAT ACGATCACTG TCCCCTTTTC GAAGACACAG TTGGGGGCCG 
GGCAAAAATT TGGCAGGGGA GACAGAGATG AGTCAGCGCA GTGCGGCACA AGGTGAAGTG 
GAGCGCAGAT CTGTTGGTCA TAGGAGAAGC ATGAGCTGGT GTCCTTCTGT TGTCCTGCGC 
TCTGAAAATG ATGAAATGAA TGAAAGAAGT TACAGTCTGG AAGGACTTGC ACCAGAGAAG 
GAGGGAAGGA AAAGTCTGGT TCAGCAGCGT GTCGGAGAGT CCAGCCAGGA GCCCAAATGT 
CCATCAAAGG TGGAAAAAGA AGACAGAGGC TCGCTTGTGT CTCTGACTGA AGAAGAGCAG 
GAGTCAGATC TAGGCGACTG CAGCAGTTTG GGCAGTCAGA AGTCCATCCA GTCGGGTCGG 
AGATGTGTCC CACATTCACA ACCCATCCTC ACCAAATCTG TGTCCATGGT GGCCATCAGT 
CAGAAGGACC TGGACGCAAT GGGACGGCTG CGACCCAAGC GACGAATCTC CTTCTCCTTC 
AGCATCTCGC CGATGCTCCC CAAATCTAAA ACTCTCTTTT CTCTTGGCTC TTCATCCAGT 
GATGAGGAGG AGGCAGTCAG ATTGAGTTCA TTCACTGGAA CATCAGGATC ATTAGAGTAC 
AGTATATCAG AGGAGGATCC TGGGCCATTA CGGAGTGACA GTGAAGGCAA AATTGGAGGA 
ACCAAAGTGA GCAGGACATT CAGTTACCTC AAGAGCAAGA TGAGCAAGAA GAACAAGGAG 
AAGGAGAAAG AGAAGAATAA GGAGCGTGAG CGAGATGCCA AAGACAAGGA GAGAAGAGCC 
TCAAACGGAC ATCTCTTCAC TACGGTCATG ACTGTACCCA CAATCCCCTG CCACCAGTGC 
AACAAACTCA TCAACACTAA AGACTCTATC CTCTGCACCA ATTGCAATGC ACAAGTCCAC 
AAAGGCTGCA AAGAGAGTCT TCCTGTCTGC GCTAAAGTCA AGATGAAGCA GCAAAAACAG 
CAGCAGACTG TTACAGATTC TGCCTCGACG CCTAGTGTCA CCCTCAGAAG TAAAACTCTT 
CCAGCAAGGG ATCGTCCGTG GTCCACAATA TCCATTCCCG ATGACCAGCC GGCTCCCCTC 
GCTCCTCCTC GGAAAAGCCC CAGCAGCATC ATGTCTTTCA ACAGCAACCC ACTGTCCAAG 
AGCATGTCCA TTAATAACAT AGCAGGACAA ATGGACGACA CTCCTCTTAA GAAGTTTCTG 
TCCCAGTCAA CGGACTCTCT TCACAAGACC AGTAAAGTCA CGGAGTCTAC AGAATCTCTT 
ACTGATGAGG GTACAGAGAT GATGGACAGC CAGCTGATGG GAGAATTTGA AGCTGACGCC 
AAAGAGCTGG AGGCTGATTC TTATAGCTTC ACGGTAGACA AGAAATTTCT TAAGCAGCTT 
AAAAAGGATG TTATCAAAAG GCAAGACGTC ATCTATGAGC TCATCCAGAC AGAAATGCAC 
CATGTAAGGA CCTTAAAGAT CATGGCTGAT GTTTACAGCA AAGGACTACT GAGAGAAGTT 
CAACTAGAAG CTCAGACGGT GGAGAAGATG TTCCCCATGT TGGACGATGT TCTGGATTTG 
CACACACAGT TCTTTTCACA CCTGCTTGAA AGAAAGAAGG AATCAGGGAG CCAAGAAGAG 
GGCGGCTTTG TCATCCAAAG GATTGGCGAT GTGCTGGTTT CTCAGTTCTC AGGTTCCAGT 
GCTGAGAGAA TGAAGAAGGT CTATGGGAAG TTCTGCGGCA GGCACAATGA AGCTGTGAAC 
TTCTACAAGG AGCTTCACAC CAAAGACAAA CGCTTTCAAG CTTTCATCAA GAAAAAGATG 
AGCAGCCCTG TTGTCCGACG GCTAAGCATT CCTGAGTGTA TATTGTTAGT CACACAACGA 
ATCACAAAAT ATCCCGTGTT ACTACAGAGA ATACTGCAGC ACACGAAAGA AAACGAGGAG 
GACCATGCAG ACGTTAGTCA AGGTCTGAAG CTGGTGAAGG AGGTGATTGC TGCCGTGGAC 
AATAAAGTGA ATGAGCATGA GAAGAAGAAG AGGCTAAAGG AAGTGTACAG CCGGACGGAC 
AACAAGTCCA TCATGAGGAT GAAGAGCGGG CAGATGTTTG CCAGAGAGGA TCTGTTACGT 
GGGCGGAAGT TAATTCACGA CGGGCCGCTG CAGCTCAAGA ACTCAGCTGG CAGACTCAAG 
GATGTCCAGG CTCTGCTGCT GAGGGACGTG ATTGTGTTCC TGCAGGAGAA AGATCAGAAG 
TACATTTTCG CCTCTTTGGA CCAGCGCTCC ACCGTCATCT CCCTGCAGAA GCTCATTGTG 
AGGGAAGTAG CCAATGAGGA GAAAGCTCTG TTTCTGATCA CAGCAGGCAT CGAGAAGCCC 
GAGATGGTAG AAGTGTACAC AAGCTCCAAA GAGGAGAGAA ACACATGGAT GCAGCTCATT 
CAGGAGGCCA TGCAGTCTAT ACTGTCCATT TCCTCCAGGG AGAGAGATGA TGATGAAGGT 
GTGCCAAGTG AAAACGAAGA GGACAGGAGA CTTCAGGAAA TCAAGGTCAA GGAGTTCAGA 
GATCAGCTGC ACAAAAGAGA CGAGCAGATC TTGACGTTAC TGGAGGAGAA GGTGAAGTTG 
TTCAGGGACA TGTGTGACTG TGGTGCACCG GACGAGACGG GGCTTCGTAA TCGAATGCTC 
TTCAGGGCCA CGACGGATGA TGTCACCAGA GGAGAGCCAA TCATGAAGGA GGCTCTGAAG 
GAAGTTGAAA CGCTGCAGGT TCTGGTTAAT GACAGTCTTG GCGGTGCGGT TCAGGAAGGT 
GTGTGCGCGG CTGGACATGT GACTTTGCCA CGACGAGCGG AAACATTTGG AGGTTTCGAC 
AGTCACCAGA TGAACATCTC TAAGAGCAAA GATGGAGACA GAGAGGAGCT GGAGGAGTCT 
GCAGAGCTCC GCAGGACTGA GTCAGACAGT GTGTTGAAGA AGACGAGTAA GACCAACCCT 
TTACTGCTGG TCAGGAGAAA CAATGAGCAG GTTCTTCAGA GCGTTGCTCA TCTTCATGAC 
CTGCTGAACA CATTACAAGC TGTGGTGGTC CAGCAGGACA CCTTCATCGA AGACCAGCGT 
CACGCTCATC CCTCCTCACG CCACCCATCC ATCTCCTCCT CTACCTCCTC TTTATCATCC 
ACCTCATCCC GGCCGAGCTC TCTAATCGAG CAGGAGAAAC AGCGCAGCCT GGAGAAGCAG 
CGGCAGGAGG TGGCCAGCCT CCAAAAACAG CAGGCGGCCC ACGCCGAGGA GAGGCGGCGC 
AGGGAAAAGC AGTGGGAGAC CCGAGAGAAA GAGCTAACCC AGAGGGAGAT GCAGATCCAG 
AGCCAAGAAG AGGAGGCGCA GAGGAGAGCC AGACAGCTGG AGGAGGAGAA ACAGGAGCTG 
CAGAGTAAGA AAGAGGAGTA TCAGAGAGAC CTGGCACGAC TGCGGGACAG CCAGAAGAGA 
CTGGAGAGAG AGAGAGAGCA GGTGCAGAGA GAGGCAGATG AGCTTAAACT GACTGAGGAA 
GTGACAAAGA ACCGGTCTCC GTCAACCACG TCTGAAGACT CCACGAAGTT CCAGAGCACC 
GGTTCATTAG ACCGTGACCC ATCAGAGATG GAGCTCTCCT CCTCCCCGTC AGCCAGAGAC 
TTCCTGTCCC GCATGGACTC CAAACGCAAA GGGAAAAACC TCAATCCATT CTCATCCAAC 
TCAAGCCAGA AGAGCCAGAA CAGCGAGGCT CAGAGCCAGA TGCCCAGCCG TCTGCTCCAG 
CTGGCCAAAC CCAAGGAGAA GAAGGACAAG AAGAAGAAGA AGGGGAAAGG CCAGCAGAGC 
CAGACTGCAG ACTCTCAGAG CACTGTTGTT CCAGGCGCTA CCCCAGACGG AGAGATCTTT 
TTCTGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

wu:fi15d04 ( XM_009297836.2 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_009296111.1 Danio rerio wu:fi15d04 (wu:fi15d04), transcript variant X3, mRNA.

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CloneID	ODa39930
Clone ID Related Accession (Same CDS sequence)	XM_009297836.2
Accession Version	XM_009297836.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7689bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-23
Organism	Danio rerio(Zebrafish)
Product	A-kinase anchor protein 13 isoform X3
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007136.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_009297836.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAGGGTG TTCCACAAGT GGCCAGTGCC ATGAAACTGA ATCCACAGCA AGCCCCATTA 
TATGGAGAGT GTCTGCTGAC GGTCCAGCTC TGTGAGGATG AGTGTGTTGA GGATGAGGAT 
GATGTGGAGT TTTACCTGCT CTTTGCTGGA TCCACACAGA GACATGTGTC CAGCACACTG 
AAGATCAGCC ATGTCACGCT GCAGGCCGTC TGTCCAGCTC ATGATCGCAG TGAAACGGTA 
CGGGTCACAC TATGCCAGGC TCGTCCCGGC GGCTCAGTGG ACCCAGTGTC CGAGGAGTGT 
TTCCAGTTTG TCCAGGACCT GGCTCTGGAC ATGGCCCAGT TCCTGGTAAA TGCGGCGGGG 
AAAACAGAAG GAGCGCTGCT GCTGGACGAG TGTCAGATCC CACTGAAAGA GTGCGAGCGG 
CTGGACGATA CTCTTGCTCT GGCTCTCCGG CATCTGCCTT TGCCTGCTGG ATGGAGCGTT 
TTGGGCACAG ATTTGGACAC GGGCACTTGC ACAGGCTCGG GCATCGCAGA GCCTGGACCA 
CACGATACAC TTTTGCACTT CGCTGCGAAG CGCGGACTAC GCAAGGTTGC ACTCTTTCTG 
CTCCAGCAAC CAGGGGGAAG AGAGGCTCTC CGGCTTCCCA ACAAACAGGG TCGCACACCG 
GCCAAGGTCG CCCAGAAGAG GGGACACTCT CAGCTCCACA AACTGCTCAC GGAGGTTGAC 
AATCTGCCCG AGCTGGAGAC GAAAACATCT AAGTGGTGTT ACCCAGAAGG CAGAGTCCTG 
CAACACCACC CCAAGCTAAA CACTTACACC CTTACCCTGG AGGATGTGCC CAGGAGCTCC 
CCACCAAGCC TTCAGTGTGA TGTGGAGGAG CTCCAGCGTC TCATTCAGTC ACATCAGAAG 
GAAGATCCTC CAACACCGCA TAAGTCAGCA CTGCTGATTC TCAATAGAGA CTCCAGTAAT 
AGCACAGAGA CAGATGACTG CCTTCCGCCC AACTCAGTGG AACCTGAGCA TCAGGAGCAC 
AACAAACAAG AAGAAACTTG CAAATCGCTT TTAAACAGCC TTACCTTTGA GGATGGATTG 
TGTGTTCATC AAAACGGCAC AGGAGATCAT GTCCAGTTGC TGGATGGGGA CGTGGAGCCT 
GACCTGCCAG CTGTAGGAGA TACAGGGAAT GCATTAGATA AGACTGACTG TTTGCAGCGG 
CGGGTAGATG GTGATTCTGT GGCTTTCAGT GCCCCTTCCT GTGGAAACCA GCGGGAGGAA 
GCCGATATAG AGCTCTGCAT AATCAGTGCC AAGCAGGGGG TTGCTTCACA GCCTGATAAA 
GGGAAGCAAG AAGAGCAAGA GGCTTGCCAG AAGAGGTCAG AGACTGAAAA GGACTATAAC 
ACTGAATCTG GCAACCTTGG GCTTCAAGAC ATGGGCCACG CACAATCTCA GGGTCTCCTC 
CCCACGGAAA CTGGTCAGCC TACACAAAGG GAGGTGGAGA GTGAGGGGAG TACAGATGTC 
TGTTGGCGTG GGGACTTACC AGAGGAAAGG GAAGGGGACA TGAGTAGCAA CCAGGAAATA 
GACTCATATT CTTCAGCACT TTCTTCCCAT AATCTAGAGG GAAACAGCCA GCACGATGGT 
GAGACTGAGC CCCAGTTGCC TACACAATGC TCAGAGGGAA CAGATGAAGC TAAATTGACT 
TTAATTCAGG TAAATTCTGA AGTTACTTTG TCTATACGAA GCGAGGATAC CGTTATTTCT 
GACAGTGTTG ACAAGCCACA TGGTCTCTTA GAGACGGAGA CAGGAAATCT CAGCGAACAC 
ACCAAAGGCA ACACTGAGTC AGCTGCTGAT TTGGTTGCGG ATGACGCTAG TGTAAAGCAG 
AACTCTGGAT TTAGCGTTTT AGAGGGGGAT CTTTTGGAAT CATTAAGTCT ACGGCCAAAC 
ATGGCACCGG GACCAGTGCA TAAGGTTCAG CAAGGTCTGT CACCAGTTCC TGAGACTTCC 
TCTCATGTAG GTCCAGCTTT GGACCCCTTT TCTGAGACTA CAGAAAGTTT TCAACAGCCG 
AATCAATCCA CACAGCTTTT AGAAACTGCA TTAGAATGTG AGGATGTTTC ATTAAATAAC 
TCAACCGGTG ACATTAATGC AATAGCAGGG GTTTCTGTTG ACATTTCTGC AGAGGTTTCA 
CTTTGCCAGA ATGAAGGGTG TCCTCCTGAT GGAAGCAAAG AAGAGGTAGC CCCCAGTGAG 
AGGATACCAG ACCTTAAAGA GCTTACGGAA GAGCCGGAAT CAGTTGAGGA CATTATTGTC 
TCAGATGTCT CAGATTTGGA TGACAAATCT CAACCTTTTG AAGAGTCTTC TTTGCCTATC 
GACTCTGAAT GTTTTATAAG AGGTGATTTC CAAAACGATG AGTCTACAGT ACAGTCTTCT 
GAATGTACTG GAGGTGACAT AAATGTAGGA ACACTCTTAG GAGAAGAAGA GGCAACTGAA 
AAAATAGAAC AGCCAGACTC TTCACTTAAA CTGGTTGAGC TAATTCCTGA GGAATATACT 
GGAGATGCCA CAAACGAAAA TAAAAAAATT GAGCCAGAGG AACAATCTAT TTTACTGCCA 
ACTTTGCAAG GGGAATCAGG ATTTAAGGAT GTTGCCACAG AGGAACCTGT ACAGGAAAGG 
GAAATTGACT CCACTTCAGA GGAAACAATC AGCGATCTCC CTGATCCATC GGAAATCACA 
AACAATGATT GTGCTGAATC CAATGCACAC TGCCAGCTCT CAGAATCCTC CAAGGAGAGC 
GCAAACACAT CCTCAGAACC TGCTGAATGT GTGAATGACC TAGACTTGTC TTGTGCTTTA 
CCTCAAACAG AAATCGAAGG CAGCAGACCT TCTGAGGGCC CCTTAATTGA TGGAGTAACA 
ATATGTGCTG AAATTCCCAT TGATGACAAA GGTGTTGACA TGGCTACCAA TATGAGTAAG 
ACGACCATCT CTGAGGATTG TCTGTCCTCT CCTATGGACA CCACCAGCTG TTCAGTTCTG 
GAGAGCTCCT CCATGTCTGA CCCACTCAAT GCCTCATCCT CAACAGCTGA GCTCACTTCC 
CCTTTGGATA TTAATAGTGG ATTGCATCAG GATTTGGAAC ACACTTCCCC AGTGGATATA 
GACAATGGTT TGCCTGAGGA ACCGCCGCCG TCAGACACAC CTGATTGCCT GAATGGATTA 
CATCAAGATT CTCCTGATAG TTTGATGGAG CACAGTGGAA CTCCTCAGCC ATGTTCTGAG 
GAACAGTGTG TGGATCAAAT CACAGATGAC AGCACTTCAG CAGAGGATGC AGCGGACGGA 
CACCAAGACT CCTCAGTAGA CATAGCAAAT TCCGAAGCGA TCCACAGAGA GACCGAAACA 
TTTTATTCAG TTGATGCCAG TCAAGACTCT GTACTCCAGG AGAAGAAATC TTCTTCAGTG 
AGCTCAGAAA TCCCCTCTTT GACCCCCTCA CCTGAGAGCA TACGTCACAA AGAGTCGCCA 
AGCACTATGC GAGGGTCCGC CAGCGATGGA GACTCTCTGT TTAGTCAGGA TCTGGGCGAG 
GACAGCGTTT TTAATAATAA GGCTGAGGAC AGCATTACCA TCACCAGTGG CGTGTCCATG 
TCCTATTCGT CCTCCACAGA TGACTCTTCC AGCTTGGGAA CCACTTGCGC CACCCCTCAG 
ACTGGCACAG AGGCTCCTGC ATCAGACCCC TTTCAGGAAC AGCCCACATC TACTGGAGCT 
TCTGGGGAAA CAGAGGAGGA AGAGAAAAAG GACCGTCTCA CTGAGGTTCC GGAGCGATCG 
GCCATTTTAC GGACCACCAT ACGATCACTG TCCCCTTTTC GAAGACACAG TTGGGGGCCG 
GGCAAAAATT TGGCAGGGGA GACAGAGATG AGTCAGCGCA GTGCGGCACA AGGTGAAGTG 
GAGCGCAGAT CTGTTGGTCA TAGGAGAAGC ATGAGCTGGT GTCCTTCTGT TGTCCTGCGC 
TCTGAAAATG ATGAAATGAA TGAAAGAAGT TACAGTCTGG AAGGACTTGC ACCAGAGAAG 
GAGGGAAGGA AAAGTCTGGT TCAGCAGCGT GTCGGAGAGT CCAGCCAGGA GCCCAAATGT 
CCATCAAAGG TGGAAAAAGA AGACAGAGGC TCGCTTGTGT CTCTGACTGA AGAAGAGCAG 
GAGTCAGATC TAGGCGACTG CAGCAGTTTG GGCAGTCAGA AGTCCATCCA GTCGGGTCGG 
AGATGTGTCC CACATTCACA ACCCATCCTC ACCAAATCTG TGTCCATGGT GGCCATCAGT 
CAGAAGGACC TGGACGCAAT GGGACGGCTG CGACCCAAGC GACGAATCTC CTTCTCCTTC 
AGCATCTCGC CGATGCTCCC CAAATCTAAA ACTCTCTTTT CTCTTGGCTC TTCATCCAGT 
GATGAGGAGG AGGCAGTCAG ATTGAGTTCA TTCACTGGAA CATCAGGATC ATTAGAGTAC 
AGTATATCAG AGGAGGATCC TGGGCCATTA CGGAGTGACA GTGAAGGCAA AATTGGAGGA 
ACCAAAGTGA GCAGGACATT CAGTTACCTC AAGAGCAAGA TGAGCAAGAA GAACAAGCAG 
GAGAAGGAGA AAGAGAAGAA TAAGGAGCGT GAGCGAGATG CCAAAGACAA GGAGAGAAGA 
GCCTCAAACG GACATCTCTT CACTACGGTC ATGACTGTAC CCACAATCCC CTGCCACCAG 
TGCAACAAAC TCATCAACAC TAAAGACTCT ATCCTCTGCA CCAATTGCAA TGCACAAGTC 
CACAAAGGCT GCAAAGAGAG TCTTCCTGTC TGCGCTAAAG TCAAGATGAA GCAAAAACAG 
CAGCAGACTG TTACAGATTC TGCCTCGACG CCTAGTGTCA CCCTCAGAAG TAAAACTCTT 
CCAGCAAGGG ATCGTCCGTG GTCCACAATA TCCATTCCCG ATGACCAGCC GGCTCCCCTC 
GCTCCTCCTC GGAAAAGCCC CAGCAGCATC ATGTCTTTCA ACAGCAACCC ACTGTCCAAG 
AGCATGTCCA TTAATAACAT AGCAGGACAA ATGGACGACA CTCCTCTTAA GAAGTTTCTG 
TCCCAGTCAA CGGACTCTCT TCACAAGACC AGTAAAGTCA CGGAGTCTAC AGAATCTCTT 
ACTGATGAGG GTACAGAGAT GATGGACAGC CAGCTGATGG GAGAATTTGA AGCTGACGCC 
AAAGAGCTGG AGGCTGATTC TTATAGCTTC ACGGTAGACA AGAAATTTCT TAAGCAGCTT 
AAAAAGGATG TTATCAAAAG GCAAGACGTC ATCTATGAGC TCATCCAGAC AGAAATGCAC 
CATGTAAGGA CCTTAAAGAT CATGGCTGAT GTTTACAGCA AAGGACTACT GAGAGAAGTT 
CAACTAGAAG CTCAGACGGT GGAGAAGATG TTCCCCATGT TGGACGATGT TCTGGATTTG 
CACACACAGT TCTTTTCACA CCTGCTTGAA AGAAAGAAGG AATCAGGGAG CCAAGAAGAG 
GGCGGCTTTG TCATCCAAAG GATTGGCGAT GTGCTGGTTT CTCAGTTCTC AGGTTCCAGT 
GCTGAGAGAA TGAAGAAGGT CTATGGGAAG TTCTGCGGCA GGCACAATGA AGCTGTGAAC 
TTCTACAAGG AGCTTCACAC CAAAGACAAA CGCTTTCAAG CTTTCATCAA GAAAAAGATG 
AGCAGCCCTG TTGTCCGACG GCTAAGCATT CCTGAGTGTA TATTGTTAGT CACACAACGA 
ATCACAAAAT ATCCCGTGTT ACTACAGAGA ATACTGCAGC ACACGAAAGA AAACGAGGAG 
GACCATGCAG ACGTTAGTCA AGGTCTGAAG CTGGTGAAGG AGGTGATTGC TGCCGTGGAC 
AATAAAGTGA ATGAGCATGA GAAGAAGAAG AGGCTAAAGG AAGTGTACAG CCGGACGGAC 
AACAAGTCCA TCATGAGGAT GAAGAGCGGG CAGATGTTTG CCAGAGAGGA TCTGTTACGT 
GGGCGGAAGT TAATTCACGA CGGGCCGCTG CAGCTCAAGA ACTCAGCTGG CAGACTCAAG 
GATGTCCAGG CTCTGCTGCT GAGGGACGTG ATTGTGTTCC TGCAGGAGAA AGATCAGAAG 
TACATTTTCG CCTCTTTGGA CCAGCGCTCC ACCGTCATCT CCCTGCAGAA GCTCATTGTG 
AGGGAAGTAG CCAATGAGGA GAAAGCTCTG TTTCTGATCA CAGCAGGCAT CGAGAAGCCC 
GAGATGGTAG AAGTGTACAC AAGCTCCAAA GAGGAGAGAA ACACATGGAT GCAGCTCATT 
CAGGAGGCCA TGCAGTCTAT ACTGTCCATT TCCTCCAGGG AGAGAGATGA TGATGAAGGT 
GTGCCAAGTG AAAACGAAGA GGACAGGAGA CTTCAGGAAA TCAAGGTCAA GGAGTTCAGA 
GATCAGCTGC ACAAAAGAGA CGAGCAGATC TTGACGTTAC TGGAGGAGAA GGTGAAGTTG 
TTCAGGGACA TGTGTGACTG TGGTGCACCG GACGAGACGG GGCTTCGTAA TCGAATGCTC 
TTCAGGGCCA CGACGGATGA TGTCACCAGA GGAGAGCCAA TCATGAAGGA GGCTCTGAAG 
GAAGTTGAAA CGCTGCAGGT TCTGGTTAAT GACAGTCTTG GCGGTGCGGT TCAGGAAGGT 
GTGTGCGCGG CTGGACATGT GACTTTGCCA CGACGAGCGG AAACATTTGG AGGTTTCGAC 
AGTCACCAGA TGAACATCTC TAAGAGCAAA GATGGAGACA GAGAGGAGCT GGAGGAGTCT 
GCAGAGCTCC GCAGGACTGA GTCAGACAGT GTGTTGAAGA AGACGAGTAA GACCAACCCT 
TTACTGCTGG TCAGGAGAAA CAATGAGCAG GTTCTTCAGA GCGTTGCTCA TCTTCATGAC 
CTGCTGAACA CATTACAAGC TGTGGTGGTC CAGCAGGACA CCTTCATCGA AGACCAGCGT 
CACGCTCATC CCTCCTCACG CCACCCATCC ATCTCCTCCT CTACCTCCTC TTTATCATCC 
ACCTCATCCC GGCCGAGCTC TCTAATCGAG CAGGAGAAAC AGCGCAGCCT GGAGAAGCAG 
CGGCAGGAGG TGGCCAGCCT CCAAAAACAG CAGGCGGCCC ACGCCGAGGA GAGGCGGCGC 
AGGGAAAAGC AGTGGGAGAC CCGAGAGAAA GAGCTAACCC AGAGGGAGAT GCAGATCCAG 
AGCCAAGAAG AGGAGGCGCA GAGGAGAGCC AGACAGCTGG AGGAGGAGAA ACAGGAGCTG 
CAGAGTAAGA AAGAGGAGTA TCAGAGAGAC CTGGCACGAC TGCGGGACAG CCAGAAGAGA 
CTGGAGAGAG AGAGAGAGCA GGTGCAGAGA GAGGCAGATG AGCTTAAACT GACTGAGGAA 
GTGACAAAGA ACCGGTCTCC GTCAACCACG TCTGAAGACT CCACGAAGTT CCAGAGCACC 
GGTTCATTAG ACCGTGACCC ATCAGAGATG GAGCTCTCCT CCTCCCCGTC AGCCAGAGAC 
TTCCTGTCCC GCATGGACTC CAAACGCAAA GGGAAAAACC TCAATCCATT CTCATCCAAC 
TCAAGCCAGA AGAGCCAGAA CAGCGAGGCT CAGAGCCAGA TGCCCAGCCG TCTGCTCCAG 
CTGGCCAAAC CCAAGGAGAA GAAGGACAAG AAGAAGAAGA AGGGGAAAGG CCAGCAGAGC 
CAGACTGCAG ACTCTCAGAG CACTGTTGTT CCAGGCGCTA CCCCAGACGG AGAGATCTTT 
TTCTGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_009296111.1
CDS	28..7716
Translation	MKGVPQVASAMKLNPQQAPLYGECLLTVQLCEDECVEDEDDVEFYLLFAGSTQRHVSSTLKISHVTLQAVCPAHDRSETVRVTLCQARPGGSVDPVSEECFQFVQDLALDMAQFLVNAAGKTEGALLLDECQIPLKECERLDDTLALALRHLPLPAGWSVLGTDLDTGTCTGSGIAEPGPHDTLLHFAAKRGLRKVALFLLQQPGGREALRLPNKQGRTPAKVAQKRGHSQLHKLLTEVDNLPELETKTSKWCYPEGRVLQHHPKLNTYTLTLEDVPRSSPPSLQCDVEELQRLIQSHQKEDPPTPHKSALLILNRDSSNSTETDDCLPPNSVEPEHQEHNKQEETCKSLLNSLTFEDGLCVHQNGTGDHVQLLDGDVEPDLPAVGDTGNALDKTDCLQRRVDGDSVAFSAPSCGNQREEADIELCIISAKQGVASQPDKGKQEEQEACQKRSETEKDYNTESGNLGLQDMGHAQSQGLLPTETGQPTQREVESEGSTDVCWRGDLPEEREGDMSSNQEIDSYSSALSSHNLEGNSQHDGETEPQLPTQCSEGTDEAKLTLIQVNSEVTLSIRSEDTVISDSVDKPHGLLETETGNLSEHTKGNTESAADLVADDASVKQNSGFSVLEGDLLESLSLRPNMAPGPVHKVQQGLSPVPETSSHVGPALDPFSETTESFQQPNQSTQLLETALECEDVSLNNSTGDINAIAGVSVDISAEVSLCQNEGCPPDGSKEEVAPSERIPDLKELTEEPESVEDIIVSDVSDLDDKSQPFEESSLPIDSECFIRGDFQNDESTVQSSECTGGDINVGTLLGEEEATEKIEQPDSSLKLVELIPEEYTGDATNENKKIEPEEQSILLPTLQGESGFKDVATEEPVQEREIDSTSEETISDLPDPSEITNNDCAESNAHCQLSESSKESANTSSEPAECVNDLDLSCALPQTEIEGSRPSEGPLIDGVTICAEIPIDDKGVDMATNMSKTTISEDCLSSPMDTTSCSVLESSSMSDPLNASSSTAELTSPLDINSGLHQDLEHTSPVDIDNGLPEEPPPSDTPDCLNGLHQDSPDSLMEHSGTPQPCSEEQCVDQITDDSTSAEDAADGHQDSSVDIANSEAIHRETETFYSVDASQDSVLQEKKSSSVSSEIPSLTPSPESIRHKESPSTMRGSASDGDSLFSQDLGEDSVFNNKAEDSITITSGVSMSYSSSTDDSSSLGTTCATPQTGTEAPASDPFQEQPTSTGASGETEEEEKKDRLTEVPERSAILRTTIRSLSPFRRHSWGPGKNLAGETEMSQRSAAQGEVERRSVGHRRSMSWCPSVVLRSENDEMNERSYSLEGLAPEKEGRKSLVQQRVGESSQEPKCPSKVEKEDRGSLVSLTEEEQESDLGDCSSLGSQKSIQSGRRCVPHSQPILTKSVSMVAISQKDLDAMGRLRPKRRISFSFSISPMLPKSKTLFSLGSSSSDEEEAVRLSSFTGTSGSLEYSISEEDPGPLRSDSEGKIGGTKVSRTFSYLKSKMSKKNKQEKEKEKNKERERDAKDKERRASNGHLFTTVMTVPTIPCHQCNKLINTKDSILCTNCNAQVHKGCKESLPVCAKVKMKQKQQQTVTDSASTPSVTLRSKTLPARDRPWSTISIPDDQPAPLAPPRKSPSSIMSFNSNPLSKSMSINNIAGQMDDTPLKKFLSQSTDSLHKTSKVTESTESLTDEGTEMMDSQLMGEFEADAKELEADSYSFTVDKKFLKQLKKDVIKRQDVIYELIQTEMHHVRTLKIMADVYSKGLLREVQLEAQTVEKMFPMLDDVLDLHTQFFSHLLERKKESGSQEEGGFVIQRIGDVLVSQFSGSSAERMKKVYGKFCGRHNEAVNFYKELHTKDKRFQAFIKKKMSSPVVRRLSIPECILLVTQRITKYPVLLQRILQHTKENEEDHADVSQGLKLVKEVIAAVDNKVNEHEKKKRLKEVYSRTDNKSIMRMKSGQMFAREDLLRGRKLIHDGPLQLKNSAGRLKDVQALLLRDVIVFLQEKDQKYIFASLDQRSTVISLQKLIVREVANEEKALFLITAGIEKPEMVEVYTSSKEERNTWMQLIQEAMQSILSISSRERDDDEGVPSENEEDRRLQEIKVKEFRDQLHKRDEQILTLLEEKVKLFRDMCDCGAPDETGLRNRMLFRATTDDVTRGEPIMKEALKEVETLQVLVNDSLGGAVQEGVCAAGHVTLPRRAETFGGFDSHQMNISKSKDGDREELEESAELRRTESDSVLKKTSKTNPLLLVRRNNEQVLQSVAHLHDLLNTLQAVVVQQDTFIEDQRHAHPSSRHPSISSSTSSLSSTSSRPSSLIEQEKQRSLEKQRQEVASLQKQQAAHAEERRRREKQWETREKELTQREMQIQSQEEEAQRRARQLEEEKQELQSKKEEYQRDLARLRDSQKRLEREREQVQREADELKLTEEVTKNRSPSTTSEDSTKFQSTGSLDRDPSEMELSSSPSARDFLSRMDSKRKGKNLNPFSSNSSQKSQNSEAQSQMPSRLLQLAKPKEKKDKKKKKGKGQQSQTADSQSTVVPGATPDGEIFFC

Target ORF information:

RefSeq Version	XM_009297836.2
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio wu:fi15d04 (wu:fi15d04), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_009297836.2

ORF Insert Sequence:

ATGAAGGGTG TTCCACAAGT GGCCAGTGCC ATGAAACTGA ATCCACAGCA AGCCCCATTA 
TATGGAGAGT GTCTGCTGAC GGTCCAGCTC TGTGAGGATG AGTGTGTTGA GGATGAGGAT 
GATGTGGAGT TTTACCTGCT CTTTGCTGGA TCCACACAGA GACATGTGTC CAGCACACTG 
AAGATCAGCC ATGTCACGCT GCAGGCCGTC TGTCCAGCTC ATGATCGCAG TGAAACGGTA 
CGGGTCACAC TATGCCAGGC TCGTCCCGGC GGCTCAGTGG ACCCAGTGTC CGAGGAGTGT 
TTCCAGTTTG TCCAGGACCT GGCTCTGGAC ATGGCCCAGT TCCTGGTAAA TGCGGCGGGG 
AAAACAGAAG GAGCGCTGCT GCTGGACGAG TGTCAGATCC CACTGAAAGA GTGCGAGCGG 
CTGGACGATA CTCTTGCTCT GGCTCTCCGG CATCTGCCTT TGCCTGCTGG ATGGAGCGTT 
TTGGGCACAG ATTTGGACAC GGGCACTTGC ACAGGCTCGG GCATCGCAGA GCCTGGACCA 
CACGATACAC TTTTGCACTT CGCTGCGAAG CGCGGACTAC GCAAGGTTGC ACTCTTTCTG 
CTCCAGCAAC CAGGGGGAAG AGAGGCTCTC CGGCTTCCCA ACAAACAGGG TCGCACACCG 
GCCAAGGTCG CCCAGAAGAG GGGACACTCT CAGCTCCACA AACTGCTCAC GGAGGTTGAC 
AATCTGCCCG AGCTGGAGAC GAAAACATCT AAGTGGTGTT ACCCAGAAGG CAGAGTCCTG 
CAACACCACC CCAAGCTAAA CACTTACACC CTTACCCTGG AGGATGTGCC CAGGAGCTCC 
CCACCAAGCC TTCAGTGTGA TGTGGAGGAG CTCCAGCGTC TCATTCAGTC ACATCAGAAG 
GAAGATCCTC CAACACCGCA TAAGTCAGCA CTGCTGATTC TCAATAGAGA CTCCAGTAAT 
AGCACAGAGA CAGATGACTG CCTTCCGCCC AACTCAGTGG AACCTGAGCA TCAGGAGCAC 
AACAAACAAG AAGAAACTTG CAAATCGCTT TTAAACAGCC TTACCTTTGA GGATGGATTG 
TGTGTTCATC AAAACGGCAC AGGAGATCAT GTCCAGTTGC TGGATGGGGA CGTGGAGCCT 
GACCTGCCAG CTGTAGGAGA TACAGGGAAT GCATTAGATA AGACTGACTG TTTGCAGCGG 
CGGGTAGATG GTGATTCTGT GGCTTTCAGT GCCCCTTCCT GTGGAAACCA GCGGGAGGAA 
GCCGATATAG AGCTCTGCAT AATCAGTGCC AAGCAGGGGG TTGCTTCACA GCCTGATAAA 
GGGAAGCAAG AAGAGCAAGA GGCTTGCCAG AAGAGGTCAG AGACTGAAAA GGACTATAAC 
ACTGAATCTG GCAACCTTGG GCTTCAAGAC ATGGGCCACG CACAATCTCA GGGTCTCCTC 
CCCACGGAAA CTGGTCAGCC TACACAAAGG GAGGTGGAGA GTGAGGGGAG TACAGATGTC 
TGTTGGCGTG GGGACTTACC AGAGGAAAGG GAAGGGGACA TGAGTAGCAA CCAGGAAATA 
GACTCATATT CTTCAGCACT TTCTTCCCAT AATCTAGAGG GAAACAGCCA GCACGATGGT 
GAGACTGAGC CCCAGTTGCC TACACAATGC TCAGAGGGAA CAGATGAAGC TAAATTGACT 
TTAATTCAGG TAAATTCTGA AGTTACTTTG TCTATACGAA GCGAGGATAC CGTTATTTCT 
GACAGTGTTG ACAAGCCACA TGGTCTCTTA GAGACGGAGA CAGGAAATCT CAGCGAACAC 
ACCAAAGGCA ACACTGAGTC AGCTGCTGAT TTGGTTGCGG ATGACGCTAG TGTAAAGCAG 
AACTCTGGAT TTAGCGTTTT AGAGGGGGAT CTTTTGGAAT CATTAAGTCT ACGGCCAAAC 
ATGGCACCGG GACCAGTGCA TAAGGTTCAG CAAGGTCTGT CACCAGTTCC TGAGACTTCC 
TCTCATGTAG GTCCAGCTTT GGACCCCTTT TCTGAGACTA CAGAAAGTTT TCAACAGCCG 
AATCAATCCA CACAGCTTTT AGAAACTGCA TTAGAATGTG AGGATGTTTC ATTAAATAAC 
TCAACCGGTG ACATTAATGC AATAGCAGGG GTTTCTGTTG ACATTTCTGC AGAGGTTTCA 
CTTTGCCAGA ATGAAGGGTG TCCTCCTGAT GGAAGCAAAG AAGAGGTAGC CCCCAGTGAG 
AGGATACCAG ACCTTAAAGA GCTTACGGAA GAGCCGGAAT CAGTTGAGGA CATTATTGTC 
TCAGATGTCT CAGATTTGGA TGACAAATCT CAACCTTTTG AAGAGTCTTC TTTGCCTATC 
GACTCTGAAT GTTTTATAAG AGGTGATTTC CAAAACGATG AGTCTACAGT ACAGTCTTCT 
GAATGTACTG GAGGTGACAT AAATGTAGGA ACACTCTTAG GAGAAGAAGA GGCAACTGAA 
AAAATAGAAC AGCCAGACTC TTCACTTAAA CTGGTTGAGC TAATTCCTGA GGAATATACT 
GGAGATGCCA CAAACGAAAA TAAAAAAATT GAGCCAGAGG AACAATCTAT TTTACTGCCA 
ACTTTGCAAG GGGAATCAGG ATTTAAGGAT GTTGCCACAG AGGAACCTGT ACAGGAAAGG 
GAAATTGACT CCACTTCAGA GGAAACAATC AGCGATCTCC CTGATCCATC GGAAATCACA 
AACAATGATT GTGCTGAATC CAATGCACAC TGCCAGCTCT CAGAATCCTC CAAGGAGAGC 
GCAAACACAT CCTCAGAACC TGCTGAATGT GTGAATGACC TAGACTTGTC TTGTGCTTTA 
CCTCAAACAG AAATCGAAGG CAGCAGACCT TCTGAGGGCC CCTTAATTGA TGGAGTAACA 
ATATGTGCTG AAATTCCCAT TGATGACAAA GGTGTTGACA TGGCTACCAA TATGAGTAAG 
ACGACCATCT CTGAGGATTG TCTGTCCTCT CCTATGGACA CCACCAGCTG TTCAGTTCTG 
GAGAGCTCCT CCATGTCTGA CCCACTCAAT GCCTCATCCT CAACAGCTGA GCTCACTTCC 
CCTTTGGATA TTAATAGTGG ATTGCATCAG GATTTGGAAC ACACTTCCCC AGTGGATATA 
GACAATGGTT TGCCTGAGGA ACCGCCGCCG TCAGACACAC CTGATTGCCT GAATGGATTA 
CATCAAGATT CTCCTGATAG TTTGATGGAG CACAGTGGAA CTCCTCAGCC ATGTTCTGAG 
GAACAGTGTG TGGATCAAAT CACAGATGAC AGCACTTCAG CAGAGGATGC AGCGGACGGA 
CACCAAGACT CCTCAGTAGA CATAGCAAAT TCCGAAGCGA TCCACAGAGA GACCGAAACA 
TTTTATTCAG TTGATGCCAG TCAAGACTCT GTACTCCAGG AGAAGAAATC TTCTTCAGTG 
AGCTCAGAAA TCCCCTCTTT GACCCCCTCA CCTGAGAGCA TACGTCACAA AGAGTCGCCA 
AGCACTATGC GAGGGTCCGC CAGCGATGGA GACTCTCTGT TTAGTCAGGA TCTGGGCGAG 
GACAGCGTTT TTAATAATAA GGCTGAGGAC AGCATTACCA TCACCAGTGG CGTGTCCATG 
TCCTATTCGT CCTCCACAGA TGACTCTTCC AGCTTGGGAA CCACTTGCGC CACCCCTCAG 
ACTGGCACAG AGGCTCCTGC ATCAGACCCC TTTCAGGAAC AGCCCACATC TACTGGAGCT 
TCTGGGGAAA CAGAGGAGGA AGAGAAAAAG GACCGTCTCA CTGAGGTTCC GGAGCGATCG 
GCCATTTTAC GGACCACCAT ACGATCACTG TCCCCTTTTC GAAGACACAG TTGGGGGCCG 
GGCAAAAATT TGGCAGGGGA GACAGAGATG AGTCAGCGCA GTGCGGCACA AGGTGAAGTG 
GAGCGCAGAT CTGTTGGTCA TAGGAGAAGC ATGAGCTGGT GTCCTTCTGT TGTCCTGCGC 
TCTGAAAATG ATGAAATGAA TGAAAGAAGT TACAGTCTGG AAGGACTTGC ACCAGAGAAG 
GAGGGAAGGA AAAGTCTGGT TCAGCAGCGT GTCGGAGAGT CCAGCCAGGA GCCCAAATGT 
CCATCAAAGG TGGAAAAAGA AGACAGAGGC TCGCTTGTGT CTCTGACTGA AGAAGAGCAG 
GAGTCAGATC TAGGCGACTG CAGCAGTTTG GGCAGTCAGA AGTCCATCCA GTCGGGTCGG 
AGATGTGTCC CACATTCACA ACCCATCCTC ACCAAATCTG TGTCCATGGT GGCCATCAGT 
CAGAAGGACC TGGACGCAAT GGGACGGCTG CGACCCAAGC GACGAATCTC CTTCTCCTTC 
AGCATCTCGC CGATGCTCCC CAAATCTAAA ACTCTCTTTT CTCTTGGCTC TTCATCCAGT 
GATGAGGAGG AGGCAGTCAG ATTGAGTTCA TTCACTGGAA CATCAGGATC ATTAGAGTAC 
AGTATATCAG AGGAGGATCC TGGGCCATTA CGGAGTGACA GTGAAGGCAA AATTGGAGGA 
ACCAAAGTGA GCAGGACATT CAGTTACCTC AAGAGCAAGA TGAGCAAGAA GAACAAGCAG 
GAGAAGGAGA AAGAGAAGAA TAAGGAGCGT GAGCGAGATG CCAAAGACAA GGAGAGAAGA 
GCCTCAAACG GACATCTCTT CACTACGGTC ATGACTGTAC CCACAATCCC CTGCCACCAG 
TGCAACAAAC TCATCAACAC TAAAGACTCT ATCCTCTGCA CCAATTGCAA TGCACAAGTC 
CACAAAGGCT GCAAAGAGAG TCTTCCTGTC TGCGCTAAAG TCAAGATGAA GCAAAAACAG 
CAGCAGACTG TTACAGATTC TGCCTCGACG CCTAGTGTCA CCCTCAGAAG TAAAACTCTT 
CCAGCAAGGG ATCGTCCGTG GTCCACAATA TCCATTCCCG ATGACCAGCC GGCTCCCCTC 
GCTCCTCCTC GGAAAAGCCC CAGCAGCATC ATGTCTTTCA ACAGCAACCC ACTGTCCAAG 
AGCATGTCCA TTAATAACAT AGCAGGACAA ATGGACGACA CTCCTCTTAA GAAGTTTCTG 
TCCCAGTCAA CGGACTCTCT TCACAAGACC AGTAAAGTCA CGGAGTCTAC AGAATCTCTT 
ACTGATGAGG GTACAGAGAT GATGGACAGC CAGCTGATGG GAGAATTTGA AGCTGACGCC 
AAAGAGCTGG AGGCTGATTC TTATAGCTTC ACGGTAGACA AGAAATTTCT TAAGCAGCTT 
AAAAAGGATG TTATCAAAAG GCAAGACGTC ATCTATGAGC TCATCCAGAC AGAAATGCAC 
CATGTAAGGA CCTTAAAGAT CATGGCTGAT GTTTACAGCA AAGGACTACT GAGAGAAGTT 
CAACTAGAAG CTCAGACGGT GGAGAAGATG TTCCCCATGT TGGACGATGT TCTGGATTTG 
CACACACAGT TCTTTTCACA CCTGCTTGAA AGAAAGAAGG AATCAGGGAG CCAAGAAGAG 
GGCGGCTTTG TCATCCAAAG GATTGGCGAT GTGCTGGTTT CTCAGTTCTC AGGTTCCAGT 
GCTGAGAGAA TGAAGAAGGT CTATGGGAAG TTCTGCGGCA GGCACAATGA AGCTGTGAAC 
TTCTACAAGG AGCTTCACAC CAAAGACAAA CGCTTTCAAG CTTTCATCAA GAAAAAGATG 
AGCAGCCCTG TTGTCCGACG GCTAAGCATT CCTGAGTGTA TATTGTTAGT CACACAACGA 
ATCACAAAAT ATCCCGTGTT ACTACAGAGA ATACTGCAGC ACACGAAAGA AAACGAGGAG 
GACCATGCAG ACGTTAGTCA AGGTCTGAAG CTGGTGAAGG AGGTGATTGC TGCCGTGGAC 
AATAAAGTGA ATGAGCATGA GAAGAAGAAG AGGCTAAAGG AAGTGTACAG CCGGACGGAC 
AACAAGTCCA TCATGAGGAT GAAGAGCGGG CAGATGTTTG CCAGAGAGGA TCTGTTACGT 
GGGCGGAAGT TAATTCACGA CGGGCCGCTG CAGCTCAAGA ACTCAGCTGG CAGACTCAAG 
GATGTCCAGG CTCTGCTGCT GAGGGACGTG ATTGTGTTCC TGCAGGAGAA AGATCAGAAG 
TACATTTTCG CCTCTTTGGA CCAGCGCTCC ACCGTCATCT CCCTGCAGAA GCTCATTGTG 
AGGGAAGTAG CCAATGAGGA GAAAGCTCTG TTTCTGATCA CAGCAGGCAT CGAGAAGCCC 
GAGATGGTAG AAGTGTACAC AAGCTCCAAA GAGGAGAGAA ACACATGGAT GCAGCTCATT 
CAGGAGGCCA TGCAGTCTAT ACTGTCCATT TCCTCCAGGG AGAGAGATGA TGATGAAGGT 
GTGCCAAGTG AAAACGAAGA GGACAGGAGA CTTCAGGAAA TCAAGGTCAA GGAGTTCAGA 
GATCAGCTGC ACAAAAGAGA CGAGCAGATC TTGACGTTAC TGGAGGAGAA GGTGAAGTTG 
TTCAGGGACA TGTGTGACTG TGGTGCACCG GACGAGACGG GGCTTCGTAA TCGAATGCTC 
TTCAGGGCCA CGACGGATGA TGTCACCAGA GGAGAGCCAA TCATGAAGGA GGCTCTGAAG 
GAAGTTGAAA CGCTGCAGGT TCTGGTTAAT GACAGTCTTG GCGGTGCGGT TCAGGAAGGT 
GTGTGCGCGG CTGGACATGT GACTTTGCCA CGACGAGCGG AAACATTTGG AGGTTTCGAC 
AGTCACCAGA TGAACATCTC TAAGAGCAAA GATGGAGACA GAGAGGAGCT GGAGGAGTCT 
GCAGAGCTCC GCAGGACTGA GTCAGACAGT GTGTTGAAGA AGACGAGTAA GACCAACCCT 
TTACTGCTGG TCAGGAGAAA CAATGAGCAG GTTCTTCAGA GCGTTGCTCA TCTTCATGAC 
CTGCTGAACA CATTACAAGC TGTGGTGGTC CAGCAGGACA CCTTCATCGA AGACCAGCGT 
CACGCTCATC CCTCCTCACG CCACCCATCC ATCTCCTCCT CTACCTCCTC TTTATCATCC 
ACCTCATCCC GGCCGAGCTC TCTAATCGAG CAGGAGAAAC AGCGCAGCCT GGAGAAGCAG 
CGGCAGGAGG TGGCCAGCCT CCAAAAACAG CAGGCGGCCC ACGCCGAGGA GAGGCGGCGC 
AGGGAAAAGC AGTGGGAGAC CCGAGAGAAA GAGCTAACCC AGAGGGAGAT GCAGATCCAG 
AGCCAAGAAG AGGAGGCGCA GAGGAGAGCC AGACAGCTGG AGGAGGAGAA ACAGGAGCTG 
CAGAGTAAGA AAGAGGAGTA TCAGAGAGAC CTGGCACGAC TGCGGGACAG CCAGAAGAGA 
CTGGAGAGAG AGAGAGAGCA GGTGCAGAGA GAGGCAGATG AGCTTAAACT GACTGAGGAA 
GTGACAAAGA ACCGGTCTCC GTCAACCACG TCTGAAGACT CCACGAAGTT CCAGAGCACC 
GGTTCATTAG ACCGTGACCC ATCAGAGATG GAGCTCTCCT CCTCCCCGTC AGCCAGAGAC 
TTCCTGTCCC GCATGGACTC CAAACGCAAA GGGAAAAACC TCAATCCATT CTCATCCAAC 
TCAAGCCAGA AGAGCCAGAA CAGCGAGGCT CAGAGCCAGA TGCCCAGCCG TCTGCTCCAG 
CTGGCCAAAC CCAAGGAGAA GAAGGACAAG AAGAAGAAGA AGGGGAAAGG CCAGCAGAGC 
CAGACTGCAG ACTCTCAGAG CACTGTTGTT CCAGGCGCTA CCCCAGACGG AGAGATCTTT 
TTCTGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

wu:fi15d04 ( XM_009297837.2 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_009296112.1 Danio rerio wu:fi15d04 (wu:fi15d04), transcript variant X4, mRNA.

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CloneID	ODa39931
Clone ID Related Accession (Same CDS sequence)	XM_009297837.2
Accession Version	XM_009297837.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7689bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-23
Organism	Danio rerio(Zebrafish)
Product	A-kinase anchor protein 13 isoform X4
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007136.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_009297837.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAGGGTG TTCCACAAGT GGCCAGTGCC ATGAAACTGA ATCCACAGCA AGCCCCATTA 
TATGGAGAGT GTCTGCTGAC GGTCCAGCTC TGTGAGGATG AGTGTGTTGA GGATGAGGAT 
GATGTGGAGT TTTACCTGCT CTTTGCTGGA TCCACACAGA GACATGTGTC CAGCACACTG 
AAGATCAGCC ATGTCACGCT GCAGGCCGTC TGTCCAGCTC ATGATCGCAG TGAAACGGTA 
CGGGTCACAC TATGCCAGGC TCGTCCCGGC GGCTCAGTGG ACCCAGTGTC CGAGGAGTGT 
TTCCAGTTTG TCCAGGACCT GGCTCTGGAC ATGGCCCAGT TCCTGGTAAA TGCGGCGGGG 
AAAACAGAAG GAGCGCTGCT GCTGGACGAG TGTCAGATCC CACTGAAAGA GTGCGAGCGG 
CTGGACGATA CTCTTGCTCT GGCTCTCCGG CATCTGCCTT TGCCTGCTGG ATGGAGCGTT 
TTGGGCACAG ATTTGGACAC GGGCACTTGC ACAGGCTCGG GCATCGCAGA GCCTGGACCA 
CACGATACAC TTTTGCACTT CGCTGCGAAG CGCGGACTAC GCAAGGTTGC ACTCTTTCTG 
CTCCAGCAAC CAGGGGGAAG AGAGGCTCTC CGGCTTCCCA ACAAACAGGG TCGCACACCG 
GCCAAGGTCG CCCAGAAGAG GGGACACTCT CAGCTCCACA AACTGCTCAC GGAGGTTGAC 
AATCTGCCCG AGCTGGAGAC GAAAACATCT AAGTGGTGTT ACCCAGAAGG CAGAGTCCTG 
CAACACCACC CCAAGCTAAA CACTTACACC CTTACCCTGG AGGATGTGCC CAGGAGCTCC 
CCACCAAGCC TTCAGTGTGA TGTGGAGGAG CTCCAGCGTC TCATTCAGTC ACATCAGAAG 
GAAGATCCTC CAACACCGCA TAAGTCAGCA CTGCTGATTC TCAATAGAGA CTCCAGTAAT 
AGCACAGAGA CAGATGACTG CCTTCCGCCC AACTCAGTGG AACCTGAGCA TCAGGAGCAC 
AACAAACAAG AAGAAACTTG CAAATCGCTT TTAAACAGCC TTACCTTTGA GGATGGATTG 
TGTGTTCATC AAAACGGCAC AGGAGATCAT GTCCAGTTGC TGGATGGGGA CGTGGAGCCT 
GACCTGCCAG CTGTAGGAGA TACAGGGAAT GCATTAGATA AGACTGACTG TTTGCAGCGG 
CGGGTAGATG GTGATTCTGT GGCTTTCAGT GCCCCTTCCT GTGGAAACCA GCGGGAGGAA 
GCCGATATAG AGCTCTGCAT AATCAGTGCC AAGCAGGGGG TTGCTTCACA GCCTGATAAA 
GGGAAGCAAG AAGAGCAAGA GGCTTGCCAG AAGAGGTCAG AGACTGAAAA GGACTATAAC 
ACTGAATCTG GCAACCTTGG GCTTCAAGAC ATGGGCCACG CACAATCTCA GGGTCTCCTC 
CCCACGGAAA CTGGTCAGCC TACACAAAGG GAGGTGGAGA GTGAGGGGAG TACAGATGTC 
TGTTGGCGTG GGGACTTACC AGAGGAAAGG GAAGGGGACA TGAGTAGCAA CCAGGAAATA 
GACTCATATT CTTCAGCACT TTCTTCCCAT AATCTAGAGG GAAACAGCCA GCACGATGGT 
GAGACTGAGC CCCAGTTGCC TACACAATGC TCAGAGGGAA CAGATGAAGC TAAATTGACT 
TTAATTCAGG TAAATTCTGA AGTTACTTTG TCTATACGAA GCGAGGATAC CGTTATTTCT 
GACAGTGTTG ACAAGCCACA TGGTCTCTTA GAGACGGAGA CAGGAAATCT CAGCGAACAC 
ACCAAAGGCA ACACTGAGTC AGCTGCTGAT TTGGTTGCGG ATGACGCTAG TGTAAAGCAG 
AACTCTGGAT TTAGCGTTTT AGAGGGGGAT CTTTTGGAAT CATTAAGTCT ACGGCCAAAC 
ATGGCACCGG GACCAGTGCA TAAGGTTCAG CAAGGTCTGT CACCAGTTCC TGAGACTTCC 
TCTCATGTAG GTCCAGCTTT GGACCCCTTT TCTGAGACTA CAGAAAGTTT TCAACAGCCG 
AATCAATCCA CACAGCTTTT AGAAACTGCA TTAGAATGTG AGGATGTTTC ATTAAATAAC 
TCAACCGGTG ACATTAATGC AATAGCAGGG GTTTCTGTTG ACATTTCTGC AGAGGTTTCA 
CTTTGCCAGA ATGAAGGGTG TCCTCCTGAT GGAAGCAAAG AAGAGGTAGC CCCCAGTGAG 
AGGATACCAG ACCTTAAAGA GCTTACGGAA GAGCCGGAAT CAGTTGAGGA CATTATTGTC 
TCAGATGTCT CAGATTTGGA TGACAAATCT CAACCTTTTG AAGAGTCTTC TTTGCCTATC 
GACTCTGAAT GTTTTATAAG AGGTGATTTC CAAAACGATG AGTCTACAGT ACAGTCTTCT 
GAATGTACTG GAGGTGACAT AAATGTAGGA ACACTCTTAG GAGAAGAAGA GGCAACTGAA 
AAAATAGAAC AGCCAGACTC TTCACTTAAA CTGGTTGAGC TAATTCCTGA GGAATATACT 
GGAGATGCCA CAAACGAAAA TAAAAAAATT GAGCCAGAGG AACAATCTAT TTTACTGCCA 
ACTTTGCAAG GGGAATCAGG ATTTAAGGAT GTTGCCACAG AGGAACCTGT ACAGGAAAGG 
GAAATTGACT CCACTTCAGA GGAAACAATC AGCGATCTCC CTGATCCATC GGAAATCACA 
AACAATGATT GTGCTGAATC CAATGCACAC TGCCAGCTCT CAGAATCCTC CAAGGAGAGC 
GCAAACACAT CCTCAGAACC TGCTGAATGT GTGAATGACC TAGACTTGTC TTGTGCTTTA 
CCTCAAACAG AAATCGAAGG CAGCAGACCT TCTGAGGGCC CCTTAATTGA TGGAGTAACA 
ATATGTGCTG AAATTCCCAT TGATGACAAA GGTGTTGACA TGGCTACCAA TATGAGTAAG 
ACGACCATCT CTGAGGATTG TCTGTCCTCT CCTATGGACA CCACCAGCTG TTCAGTTCTG 
GAGAGCTCCT CCATGTCTGA CCCACTCAAT GCCTCATCCT CAACAGCTGA GCTCACTTCC 
CCTTTGGATA TTAATAGTGG ATTGCATCAG GATTTGGAAC ACACTTCCCC AGTGGATATA 
GACAATGGTT TGCCTGAGGA ACCGCCGCCG TCAGACACAC CTGATTGCCT GAATGGATTA 
CATCAAGATT CTCCTGATAG TTTGATGGAG CACAGTGGAA CTCCTCAGCC ATGTTCTGAG 
GAACAGTGTG TGGATCAAAT CACAGATGAC AGCACTTCAG CAGAGGATGC AGCGGACGGA 
CACCAAGACT CCTCAGTAGA CATAGCAAAT TCCGAAGCGA TCCACAGAGA GACCGAAACA 
TTTTATTCAG TTGATGCCAG TCAAGACTCT GTACTCCAGG AGAAGAAATC TTCTTCAGTG 
AGCTCAGAAA TCCCCTCTTT GACCCCCTCA CCTGAGAGCA TACGTCACAA AGAGTCGCCA 
AGCACTATGC GAGGGTCCGC CAGCGATGGA GACTCTCTGT TTAGTCAGGA TCTGGGCGAG 
GACAGCGTTT TTAATAATAA GGCTGAGGAC AGCATTACCA TCACCAGTGG CGTGTCCATG 
TCCTATTCGT CCTCCACAGA TGACTCTTCC AGCTTGGGAA CCACTTGCGC CACCCCTCAG 
ACTGGCACAG AGGCTCCTGC ATCAGACCCC TTTCAGGAAC AGCCCACATC TACTGGAGCT 
TCTGGGGAAA CAGAGGAGGA AGAGAAAAAG GACCGTCTCA CTGAGGTTCC GGAGCGATCG 
GCCATTTTAC GGACCACCAT ACGATCACTG TCCCCTTTTC GAAGACACAG TTGGGGGCCG 
GGCAAAAATT TGGCAGGGGA GACAGAGATG AGTCAGCGCA GTGCGGCACA AGGTGAAGTG 
GAGCGCAGAT CTGTTGGTCA TAGGAGAAGC ATGAGCTGGT GTCCTTCTGT TGTCCTGCGC 
TCTGAAAATG ATGAAATGAA TGAAAGAAGT TACAGTCTGG AAGGACTTGC ACCAGAGAAG 
GAGGGAAGGA AAAGTCTGGT TCAGCAGCGT GTCGGAGAGT CCAGCCAGGA GCCCAAATGT 
CCATCAAAGG TGGAAAAAGA AGACAGAGGC TCGCTTGTGT CTCTGACTGA AGAAGAGCAG 
GAGTCAGATC TAGGCGACTG CAGCAGTTTG GGCAGTCAGA AGTCCATCCA GTCGGGTCGG 
AGATGTGTCC CACATTCACA ACCCATCCTC ACCAAATCTG TGTCCATGGT GGCCATCAGT 
CAGAAGGACC TGGACGCAAT GGGACGGCTG CGACCCAAGC GACGAATCTC CTTCTCCTTC 
AGCATCTCGC CGATGCTCCC CAAATCTAAA ACTCTCTTTT CTCTTGGCTC TTCATCCAGT 
GATGAGGAGG AGGCAGTCAG ATTGAGTTCA TTCACTGGAA CATCAGGATC ATTAGAGTAC 
AGTATATCAG AGGAGGATCC TGGGCCATTA CGGAGTGACA GTGAAGGCAA AATTGGAGGA 
ACCAAAGTGA GCAGGACATT CAGTTACCTC AAGAGCAAGA TGAGCAAGAA GAACAAGCAG 
GAGAAGGAGA AAGAGAAGAA TAAGGAGCGT GAGCGAGATG CCAAAGACAA GGAGAGAAGA 
GCCTCAAACG GACATCTCTT CACTACGGTC ATGACTGTAC CCACAATCCC CTGCCACCAG 
TGCAACAAAC TCATCAACAC TAAAGACTCT ATCCTCTGCA CCAATTGCAA TGCACAAGTC 
CACAAAGGCT GCAAAGAGAG TCTTCCTGTC TGCGCTAAAG TCAAGATGAA GCAGCAAAAA 
CAGCAGCAGA CTGTTACAGA TTCTGCCTCG ACGCCTAGTG TCACCCTCAG AAGTAAAACT 
CTTCCAGCAA GGGATCGTCC GTGGTCCACA ATATCCATTC CCGATGACCA GCCGGCTCCC 
CTCGCTCCTC CTCGGAAAAG CCCCAGCAGC ATCATGTCTT TCAACAGCAA CCCACTGTCC 
AAGAGCATGT CCATTAATAA CATAGCAGGA CAAATGGACG ACACTCCTCT TAAGAAGTTT 
CTGTCCCAGT CAACGGACTC TCTTCACAAG ACCAGTAAAG TCACGGAGTC TACAGAATCT 
CTTACTGATG AGGGTACAGA GATGATGGAC AGCCAGCTGA TGGGAGAATT TGAAGCTGAC 
GCCAAAGAGC TGGAGGCTGA TTCTTATAGC TTCACGGTAG ACAAGAAATT TCTTAAGCAG 
CTTAAAAAGG ATGTTATCAA AAGGCAAGAC GTCATCTATG AGCTCATCCA GACAGAAATG 
CACCATGTAA GGACCTTAAA GATCATGGCT GATGTTTACA GCAAAGGACT ACTGAGAGAA 
GTTCAACTAG AAGCTCAGAC GGTGGAGAAG ATGTTCCCCA TGTTGGACGA TGTTCTGGAT 
TTGCACACAC AGTTCTTTTC ACACCTGCTT GAAAGAAAGA AGGAATCAGG GAGCCAAGAA 
GAGGGCGGCT TTGTCATCCA AAGGATTGGC GATGTGCTGG TTTCTCAGTT CTCAGGTTCC 
AGTGCTGAGA GAATGAAGAA GGTCTATGGG AAGTTCTGCG GCAGGCACAA TGAAGCTGTG 
AACTTCTACA AGGAGCTTCA CACCAAAGAC AAACGCTTTC AAGCTTTCAT CAAGAAAAAG 
ATGAGCAGCC CTGTTGTCCG ACGGCTAAGC ATTCCTGAGT GTATATTGTT AGTCACACAA 
CGAATCACAA AATATCCCGT GTTACTACAG AGAATACTGC AGCACACGAA AGAAAACGAG 
GAGGACCATG CAGACGTTAG TCAAGGTCTG AAGCTGGTGA AGGAGGTGAT TGCTGCCGTG 
GACAATAAAG TGAATGAGCA TGAGAAGAAG AAGAGGCTAA AGGAAGTGTA CAGCCGGACG 
GACAACAAGT CCATCATGAG GATGAAGAGC GGGCAGATGT TTGCCAGAGA GGATCTGTTA 
CGTGGGCGGA AGTTAATTCA CGACGGGCCG CTGCAGCTCA AGAACTCAGC TGGCAGACTC 
AAGGATGTCC AGGCTCTGCT GCTGAGGGAC GTGATTGTGT TCCTGCAGGA GAAAGATCAG 
AAGTACATTT TCGCCTCTTT GGACCAGCGC TCCACCGTCA TCTCCCTGCA GAAGCTCATT 
GTGAGGGAAG TAGCCAATGA GGAGAAAGCT CTGTTTCTGA TCACAGCAGG CATCGAGAAG 
CCCGAGATGG TAGAAGTGTA CACAAGCTCC AAAGAGGAGA GAAACACATG GATGCAGCTC 
ATTCAGGAGG CCATGCAGTC TATACTGTCC ATTTCCTCCA GGGAGAGAGA TGATGATGAA 
GGTGTGCCAA GTGAAAACGA AGAGGACAGG AGACTTCAGG AAATCAAGGT CAAGGAGTTC 
AGAGATCAGC TGCACAAAAG AGACGAGCAG ATCTTGACGT TACTGGAGGA GAAGGTGAAG 
TTGTTCAGGG ACATGTGTGA CTGTGGTGCA CCGGACGAGA CGGGGCTTCG TAATCGAATG 
CTCTTCAGGG CCACGACGGA TGATGTCACC AGAGGAGAGC CAATCATGAA GGAGGCTCTG 
AAGGAAGTTG AAACGCTGCA GGTTCTGGTT AATGACAGTC TTGGCGGTGC GGTTCAGGAA 
GGTGTGTGCG CGGCTGGACA TGTGACTTTG CCACGACGAG CGGAAACATT TGGAGGTTTC 
GACAGTCACC AGATGAACAT CTCTAAGAGC AAAGATGGAG ACAGAGAGGA GCTGGAGGAG 
TCTGCAGAGC TCCGCAGGAC TGAGTCAGAC AGTGTGTTGA AGAAGACGAG TAAGACCAAC 
CCTTTACTGC TGGTCAGGAG AAACAATGAG GTTCTTCAGA GCGTTGCTCA TCTTCATGAC 
CTGCTGAACA CATTACAAGC TGTGGTGGTC CAGCAGGACA CCTTCATCGA AGACCAGCGT 
CACGCTCATC CCTCCTCACG CCACCCATCC ATCTCCTCCT CTACCTCCTC TTTATCATCC 
ACCTCATCCC GGCCGAGCTC TCTAATCGAG CAGGAGAAAC AGCGCAGCCT GGAGAAGCAG 
CGGCAGGAGG TGGCCAGCCT CCAAAAACAG CAGGCGGCCC ACGCCGAGGA GAGGCGGCGC 
AGGGAAAAGC AGTGGGAGAC CCGAGAGAAA GAGCTAACCC AGAGGGAGAT GCAGATCCAG 
AGCCAAGAAG AGGAGGCGCA GAGGAGAGCC AGACAGCTGG AGGAGGAGAA ACAGGAGCTG 
CAGAGTAAGA AAGAGGAGTA TCAGAGAGAC CTGGCACGAC TGCGGGACAG CCAGAAGAGA 
CTGGAGAGAG AGAGAGAGCA GGTGCAGAGA GAGGCAGATG AGCTTAAACT GACTGAGGAA 
GTGACAAAGA ACCGGTCTCC GTCAACCACG TCTGAAGACT CCACGAAGTT CCAGAGCACC 
GGTTCATTAG ACCGTGACCC ATCAGAGATG GAGCTCTCCT CCTCCCCGTC AGCCAGAGAC 
TTCCTGTCCC GCATGGACTC CAAACGCAAA GGGAAAAACC TCAATCCATT CTCATCCAAC 
TCAAGCCAGA AGAGCCAGAA CAGCGAGGCT CAGAGCCAGA TGCCCAGCCG TCTGCTCCAG 
CTGGCCAAAC CCAAGGAGAA GAAGGACAAG AAGAAGAAGA AGGGGAAAGG CCAGCAGAGC 
CAGACTGCAG ACTCTCAGAG CACTGTTGTT CCAGGCGCTA CCCCAGACGG AGAGATCTTT 
TTCTGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_009296112.1
CDS	28..7716
Translation	MKGVPQVASAMKLNPQQAPLYGECLLTVQLCEDECVEDEDDVEFYLLFAGSTQRHVSSTLKISHVTLQAVCPAHDRSETVRVTLCQARPGGSVDPVSEECFQFVQDLALDMAQFLVNAAGKTEGALLLDECQIPLKECERLDDTLALALRHLPLPAGWSVLGTDLDTGTCTGSGIAEPGPHDTLLHFAAKRGLRKVALFLLQQPGGREALRLPNKQGRTPAKVAQKRGHSQLHKLLTEVDNLPELETKTSKWCYPEGRVLQHHPKLNTYTLTLEDVPRSSPPSLQCDVEELQRLIQSHQKEDPPTPHKSALLILNRDSSNSTETDDCLPPNSVEPEHQEHNKQEETCKSLLNSLTFEDGLCVHQNGTGDHVQLLDGDVEPDLPAVGDTGNALDKTDCLQRRVDGDSVAFSAPSCGNQREEADIELCIISAKQGVASQPDKGKQEEQEACQKRSETEKDYNTESGNLGLQDMGHAQSQGLLPTETGQPTQREVESEGSTDVCWRGDLPEEREGDMSSNQEIDSYSSALSSHNLEGNSQHDGETEPQLPTQCSEGTDEAKLTLIQVNSEVTLSIRSEDTVISDSVDKPHGLLETETGNLSEHTKGNTESAADLVADDASVKQNSGFSVLEGDLLESLSLRPNMAPGPVHKVQQGLSPVPETSSHVGPALDPFSETTESFQQPNQSTQLLETALECEDVSLNNSTGDINAIAGVSVDISAEVSLCQNEGCPPDGSKEEVAPSERIPDLKELTEEPESVEDIIVSDVSDLDDKSQPFEESSLPIDSECFIRGDFQNDESTVQSSECTGGDINVGTLLGEEEATEKIEQPDSSLKLVELIPEEYTGDATNENKKIEPEEQSILLPTLQGESGFKDVATEEPVQEREIDSTSEETISDLPDPSEITNNDCAESNAHCQLSESSKESANTSSEPAECVNDLDLSCALPQTEIEGSRPSEGPLIDGVTICAEIPIDDKGVDMATNMSKTTISEDCLSSPMDTTSCSVLESSSMSDPLNASSSTAELTSPLDINSGLHQDLEHTSPVDIDNGLPEEPPPSDTPDCLNGLHQDSPDSLMEHSGTPQPCSEEQCVDQITDDSTSAEDAADGHQDSSVDIANSEAIHRETETFYSVDASQDSVLQEKKSSSVSSEIPSLTPSPESIRHKESPSTMRGSASDGDSLFSQDLGEDSVFNNKAEDSITITSGVSMSYSSSTDDSSSLGTTCATPQTGTEAPASDPFQEQPTSTGASGETEEEEKKDRLTEVPERSAILRTTIRSLSPFRRHSWGPGKNLAGETEMSQRSAAQGEVERRSVGHRRSMSWCPSVVLRSENDEMNERSYSLEGLAPEKEGRKSLVQQRVGESSQEPKCPSKVEKEDRGSLVSLTEEEQESDLGDCSSLGSQKSIQSGRRCVPHSQPILTKSVSMVAISQKDLDAMGRLRPKRRISFSFSISPMLPKSKTLFSLGSSSSDEEEAVRLSSFTGTSGSLEYSISEEDPGPLRSDSEGKIGGTKVSRTFSYLKSKMSKKNKQEKEKEKNKERERDAKDKERRASNGHLFTTVMTVPTIPCHQCNKLINTKDSILCTNCNAQVHKGCKESLPVCAKVKMKQQKQQQTVTDSASTPSVTLRSKTLPARDRPWSTISIPDDQPAPLAPPRKSPSSIMSFNSNPLSKSMSINNIAGQMDDTPLKKFLSQSTDSLHKTSKVTESTESLTDEGTEMMDSQLMGEFEADAKELEADSYSFTVDKKFLKQLKKDVIKRQDVIYELIQTEMHHVRTLKIMADVYSKGLLREVQLEAQTVEKMFPMLDDVLDLHTQFFSHLLERKKESGSQEEGGFVIQRIGDVLVSQFSGSSAERMKKVYGKFCGRHNEAVNFYKELHTKDKRFQAFIKKKMSSPVVRRLSIPECILLVTQRITKYPVLLQRILQHTKENEEDHADVSQGLKLVKEVIAAVDNKVNEHEKKKRLKEVYSRTDNKSIMRMKSGQMFAREDLLRGRKLIHDGPLQLKNSAGRLKDVQALLLRDVIVFLQEKDQKYIFASLDQRSTVISLQKLIVREVANEEKALFLITAGIEKPEMVEVYTSSKEERNTWMQLIQEAMQSILSISSRERDDDEGVPSENEEDRRLQEIKVKEFRDQLHKRDEQILTLLEEKVKLFRDMCDCGAPDETGLRNRMLFRATTDDVTRGEPIMKEALKEVETLQVLVNDSLGGAVQEGVCAAGHVTLPRRAETFGGFDSHQMNISKSKDGDREELEESAELRRTESDSVLKKTSKTNPLLLVRRNNEVLQSVAHLHDLLNTLQAVVVQQDTFIEDQRHAHPSSRHPSISSSTSSLSSTSSRPSSLIEQEKQRSLEKQRQEVASLQKQQAAHAEERRRREKQWETREKELTQREMQIQSQEEEAQRRARQLEEEKQELQSKKEEYQRDLARLRDSQKRLEREREQVQREADELKLTEEVTKNRSPSTTSEDSTKFQSTGSLDRDPSEMELSSSPSARDFLSRMDSKRKGKNLNPFSSNSSQKSQNSEAQSQMPSRLLQLAKPKEKKDKKKKKGKGQQSQTADSQSTVVPGATPDGEIFFC

Target ORF information:

RefSeq Version	XM_009297837.2
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio wu:fi15d04 (wu:fi15d04), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_009297837.2

ORF Insert Sequence:

ATGAAGGGTG TTCCACAAGT GGCCAGTGCC ATGAAACTGA ATCCACAGCA AGCCCCATTA 
TATGGAGAGT GTCTGCTGAC GGTCCAGCTC TGTGAGGATG AGTGTGTTGA GGATGAGGAT 
GATGTGGAGT TTTACCTGCT CTTTGCTGGA TCCACACAGA GACATGTGTC CAGCACACTG 
AAGATCAGCC ATGTCACGCT GCAGGCCGTC TGTCCAGCTC ATGATCGCAG TGAAACGGTA 
CGGGTCACAC TATGCCAGGC TCGTCCCGGC GGCTCAGTGG ACCCAGTGTC CGAGGAGTGT 
TTCCAGTTTG TCCAGGACCT GGCTCTGGAC ATGGCCCAGT TCCTGGTAAA TGCGGCGGGG 
AAAACAGAAG GAGCGCTGCT GCTGGACGAG TGTCAGATCC CACTGAAAGA GTGCGAGCGG 
CTGGACGATA CTCTTGCTCT GGCTCTCCGG CATCTGCCTT TGCCTGCTGG ATGGAGCGTT 
TTGGGCACAG ATTTGGACAC GGGCACTTGC ACAGGCTCGG GCATCGCAGA GCCTGGACCA 
CACGATACAC TTTTGCACTT CGCTGCGAAG CGCGGACTAC GCAAGGTTGC ACTCTTTCTG 
CTCCAGCAAC CAGGGGGAAG AGAGGCTCTC CGGCTTCCCA ACAAACAGGG TCGCACACCG 
GCCAAGGTCG CCCAGAAGAG GGGACACTCT CAGCTCCACA AACTGCTCAC GGAGGTTGAC 
AATCTGCCCG AGCTGGAGAC GAAAACATCT AAGTGGTGTT ACCCAGAAGG CAGAGTCCTG 
CAACACCACC CCAAGCTAAA CACTTACACC CTTACCCTGG AGGATGTGCC CAGGAGCTCC 
CCACCAAGCC TTCAGTGTGA TGTGGAGGAG CTCCAGCGTC TCATTCAGTC ACATCAGAAG 
GAAGATCCTC CAACACCGCA TAAGTCAGCA CTGCTGATTC TCAATAGAGA CTCCAGTAAT 
AGCACAGAGA CAGATGACTG CCTTCCGCCC AACTCAGTGG AACCTGAGCA TCAGGAGCAC 
AACAAACAAG AAGAAACTTG CAAATCGCTT TTAAACAGCC TTACCTTTGA GGATGGATTG 
TGTGTTCATC AAAACGGCAC AGGAGATCAT GTCCAGTTGC TGGATGGGGA CGTGGAGCCT 
GACCTGCCAG CTGTAGGAGA TACAGGGAAT GCATTAGATA AGACTGACTG TTTGCAGCGG 
CGGGTAGATG GTGATTCTGT GGCTTTCAGT GCCCCTTCCT GTGGAAACCA GCGGGAGGAA 
GCCGATATAG AGCTCTGCAT AATCAGTGCC AAGCAGGGGG TTGCTTCACA GCCTGATAAA 
GGGAAGCAAG AAGAGCAAGA GGCTTGCCAG AAGAGGTCAG AGACTGAAAA GGACTATAAC 
ACTGAATCTG GCAACCTTGG GCTTCAAGAC ATGGGCCACG CACAATCTCA GGGTCTCCTC 
CCCACGGAAA CTGGTCAGCC TACACAAAGG GAGGTGGAGA GTGAGGGGAG TACAGATGTC 
TGTTGGCGTG GGGACTTACC AGAGGAAAGG GAAGGGGACA TGAGTAGCAA CCAGGAAATA 
GACTCATATT CTTCAGCACT TTCTTCCCAT AATCTAGAGG GAAACAGCCA GCACGATGGT 
GAGACTGAGC CCCAGTTGCC TACACAATGC TCAGAGGGAA CAGATGAAGC TAAATTGACT 
TTAATTCAGG TAAATTCTGA AGTTACTTTG TCTATACGAA GCGAGGATAC CGTTATTTCT 
GACAGTGTTG ACAAGCCACA TGGTCTCTTA GAGACGGAGA CAGGAAATCT CAGCGAACAC 
ACCAAAGGCA ACACTGAGTC AGCTGCTGAT TTGGTTGCGG ATGACGCTAG TGTAAAGCAG 
AACTCTGGAT TTAGCGTTTT AGAGGGGGAT CTTTTGGAAT CATTAAGTCT ACGGCCAAAC 
ATGGCACCGG GACCAGTGCA TAAGGTTCAG CAAGGTCTGT CACCAGTTCC TGAGACTTCC 
TCTCATGTAG GTCCAGCTTT GGACCCCTTT TCTGAGACTA CAGAAAGTTT TCAACAGCCG 
AATCAATCCA CACAGCTTTT AGAAACTGCA TTAGAATGTG AGGATGTTTC ATTAAATAAC 
TCAACCGGTG ACATTAATGC AATAGCAGGG GTTTCTGTTG ACATTTCTGC AGAGGTTTCA 
CTTTGCCAGA ATGAAGGGTG TCCTCCTGAT GGAAGCAAAG AAGAGGTAGC CCCCAGTGAG 
AGGATACCAG ACCTTAAAGA GCTTACGGAA GAGCCGGAAT CAGTTGAGGA CATTATTGTC 
TCAGATGTCT CAGATTTGGA TGACAAATCT CAACCTTTTG AAGAGTCTTC TTTGCCTATC 
GACTCTGAAT GTTTTATAAG AGGTGATTTC CAAAACGATG AGTCTACAGT ACAGTCTTCT 
GAATGTACTG GAGGTGACAT AAATGTAGGA ACACTCTTAG GAGAAGAAGA GGCAACTGAA 
AAAATAGAAC AGCCAGACTC TTCACTTAAA CTGGTTGAGC TAATTCCTGA GGAATATACT 
GGAGATGCCA CAAACGAAAA TAAAAAAATT GAGCCAGAGG AACAATCTAT TTTACTGCCA 
ACTTTGCAAG GGGAATCAGG ATTTAAGGAT GTTGCCACAG AGGAACCTGT ACAGGAAAGG 
GAAATTGACT CCACTTCAGA GGAAACAATC AGCGATCTCC CTGATCCATC GGAAATCACA 
AACAATGATT GTGCTGAATC CAATGCACAC TGCCAGCTCT CAGAATCCTC CAAGGAGAGC 
GCAAACACAT CCTCAGAACC TGCTGAATGT GTGAATGACC TAGACTTGTC TTGTGCTTTA 
CCTCAAACAG AAATCGAAGG CAGCAGACCT TCTGAGGGCC CCTTAATTGA TGGAGTAACA 
ATATGTGCTG AAATTCCCAT TGATGACAAA GGTGTTGACA TGGCTACCAA TATGAGTAAG 
ACGACCATCT CTGAGGATTG TCTGTCCTCT CCTATGGACA CCACCAGCTG TTCAGTTCTG 
GAGAGCTCCT CCATGTCTGA CCCACTCAAT GCCTCATCCT CAACAGCTGA GCTCACTTCC 
CCTTTGGATA TTAATAGTGG ATTGCATCAG GATTTGGAAC ACACTTCCCC AGTGGATATA 
GACAATGGTT TGCCTGAGGA ACCGCCGCCG TCAGACACAC CTGATTGCCT GAATGGATTA 
CATCAAGATT CTCCTGATAG TTTGATGGAG CACAGTGGAA CTCCTCAGCC ATGTTCTGAG 
GAACAGTGTG TGGATCAAAT CACAGATGAC AGCACTTCAG CAGAGGATGC AGCGGACGGA 
CACCAAGACT CCTCAGTAGA CATAGCAAAT TCCGAAGCGA TCCACAGAGA GACCGAAACA 
TTTTATTCAG TTGATGCCAG TCAAGACTCT GTACTCCAGG AGAAGAAATC TTCTTCAGTG 
AGCTCAGAAA TCCCCTCTTT GACCCCCTCA CCTGAGAGCA TACGTCACAA AGAGTCGCCA 
AGCACTATGC GAGGGTCCGC CAGCGATGGA GACTCTCTGT TTAGTCAGGA TCTGGGCGAG 
GACAGCGTTT TTAATAATAA GGCTGAGGAC AGCATTACCA TCACCAGTGG CGTGTCCATG 
TCCTATTCGT CCTCCACAGA TGACTCTTCC AGCTTGGGAA CCACTTGCGC CACCCCTCAG 
ACTGGCACAG AGGCTCCTGC ATCAGACCCC TTTCAGGAAC AGCCCACATC TACTGGAGCT 
TCTGGGGAAA CAGAGGAGGA AGAGAAAAAG GACCGTCTCA CTGAGGTTCC GGAGCGATCG 
GCCATTTTAC GGACCACCAT ACGATCACTG TCCCCTTTTC GAAGACACAG TTGGGGGCCG 
GGCAAAAATT TGGCAGGGGA GACAGAGATG AGTCAGCGCA GTGCGGCACA AGGTGAAGTG 
GAGCGCAGAT CTGTTGGTCA TAGGAGAAGC ATGAGCTGGT GTCCTTCTGT TGTCCTGCGC 
TCTGAAAATG ATGAAATGAA TGAAAGAAGT TACAGTCTGG AAGGACTTGC ACCAGAGAAG 
GAGGGAAGGA AAAGTCTGGT TCAGCAGCGT GTCGGAGAGT CCAGCCAGGA GCCCAAATGT 
CCATCAAAGG TGGAAAAAGA AGACAGAGGC TCGCTTGTGT CTCTGACTGA AGAAGAGCAG 
GAGTCAGATC TAGGCGACTG CAGCAGTTTG GGCAGTCAGA AGTCCATCCA GTCGGGTCGG 
AGATGTGTCC CACATTCACA ACCCATCCTC ACCAAATCTG TGTCCATGGT GGCCATCAGT 
CAGAAGGACC TGGACGCAAT GGGACGGCTG CGACCCAAGC GACGAATCTC CTTCTCCTTC 
AGCATCTCGC CGATGCTCCC CAAATCTAAA ACTCTCTTTT CTCTTGGCTC TTCATCCAGT 
GATGAGGAGG AGGCAGTCAG ATTGAGTTCA TTCACTGGAA CATCAGGATC ATTAGAGTAC 
AGTATATCAG AGGAGGATCC TGGGCCATTA CGGAGTGACA GTGAAGGCAA AATTGGAGGA 
ACCAAAGTGA GCAGGACATT CAGTTACCTC AAGAGCAAGA TGAGCAAGAA GAACAAGCAG 
GAGAAGGAGA AAGAGAAGAA TAAGGAGCGT GAGCGAGATG CCAAAGACAA GGAGAGAAGA 
GCCTCAAACG GACATCTCTT CACTACGGTC ATGACTGTAC CCACAATCCC CTGCCACCAG 
TGCAACAAAC TCATCAACAC TAAAGACTCT ATCCTCTGCA CCAATTGCAA TGCACAAGTC 
CACAAAGGCT GCAAAGAGAG TCTTCCTGTC TGCGCTAAAG TCAAGATGAA GCAGCAAAAA 
CAGCAGCAGA CTGTTACAGA TTCTGCCTCG ACGCCTAGTG TCACCCTCAG AAGTAAAACT 
CTTCCAGCAA GGGATCGTCC GTGGTCCACA ATATCCATTC CCGATGACCA GCCGGCTCCC 
CTCGCTCCTC CTCGGAAAAG CCCCAGCAGC ATCATGTCTT TCAACAGCAA CCCACTGTCC 
AAGAGCATGT CCATTAATAA CATAGCAGGA CAAATGGACG ACACTCCTCT TAAGAAGTTT 
CTGTCCCAGT CAACGGACTC TCTTCACAAG ACCAGTAAAG TCACGGAGTC TACAGAATCT 
CTTACTGATG AGGGTACAGA GATGATGGAC AGCCAGCTGA TGGGAGAATT TGAAGCTGAC 
GCCAAAGAGC TGGAGGCTGA TTCTTATAGC TTCACGGTAG ACAAGAAATT TCTTAAGCAG 
CTTAAAAAGG ATGTTATCAA AAGGCAAGAC GTCATCTATG AGCTCATCCA GACAGAAATG 
CACCATGTAA GGACCTTAAA GATCATGGCT GATGTTTACA GCAAAGGACT ACTGAGAGAA 
GTTCAACTAG AAGCTCAGAC GGTGGAGAAG ATGTTCCCCA TGTTGGACGA TGTTCTGGAT 
TTGCACACAC AGTTCTTTTC ACACCTGCTT GAAAGAAAGA AGGAATCAGG GAGCCAAGAA 
GAGGGCGGCT TTGTCATCCA AAGGATTGGC GATGTGCTGG TTTCTCAGTT CTCAGGTTCC 
AGTGCTGAGA GAATGAAGAA GGTCTATGGG AAGTTCTGCG GCAGGCACAA TGAAGCTGTG 
AACTTCTACA AGGAGCTTCA CACCAAAGAC AAACGCTTTC AAGCTTTCAT CAAGAAAAAG 
ATGAGCAGCC CTGTTGTCCG ACGGCTAAGC ATTCCTGAGT GTATATTGTT AGTCACACAA 
CGAATCACAA AATATCCCGT GTTACTACAG AGAATACTGC AGCACACGAA AGAAAACGAG 
GAGGACCATG CAGACGTTAG TCAAGGTCTG AAGCTGGTGA AGGAGGTGAT TGCTGCCGTG 
GACAATAAAG TGAATGAGCA TGAGAAGAAG AAGAGGCTAA AGGAAGTGTA CAGCCGGACG 
GACAACAAGT CCATCATGAG GATGAAGAGC GGGCAGATGT TTGCCAGAGA GGATCTGTTA 
CGTGGGCGGA AGTTAATTCA CGACGGGCCG CTGCAGCTCA AGAACTCAGC TGGCAGACTC 
AAGGATGTCC AGGCTCTGCT GCTGAGGGAC GTGATTGTGT TCCTGCAGGA GAAAGATCAG 
AAGTACATTT TCGCCTCTTT GGACCAGCGC TCCACCGTCA TCTCCCTGCA GAAGCTCATT 
GTGAGGGAAG TAGCCAATGA GGAGAAAGCT CTGTTTCTGA TCACAGCAGG CATCGAGAAG 
CCCGAGATGG TAGAAGTGTA CACAAGCTCC AAAGAGGAGA GAAACACATG GATGCAGCTC 
ATTCAGGAGG CCATGCAGTC TATACTGTCC ATTTCCTCCA GGGAGAGAGA TGATGATGAA 
GGTGTGCCAA GTGAAAACGA AGAGGACAGG AGACTTCAGG AAATCAAGGT CAAGGAGTTC 
AGAGATCAGC TGCACAAAAG AGACGAGCAG ATCTTGACGT TACTGGAGGA GAAGGTGAAG 
TTGTTCAGGG ACATGTGTGA CTGTGGTGCA CCGGACGAGA CGGGGCTTCG TAATCGAATG 
CTCTTCAGGG CCACGACGGA TGATGTCACC AGAGGAGAGC CAATCATGAA GGAGGCTCTG 
AAGGAAGTTG AAACGCTGCA GGTTCTGGTT AATGACAGTC TTGGCGGTGC GGTTCAGGAA 
GGTGTGTGCG CGGCTGGACA TGTGACTTTG CCACGACGAG CGGAAACATT TGGAGGTTTC 
GACAGTCACC AGATGAACAT CTCTAAGAGC AAAGATGGAG ACAGAGAGGA GCTGGAGGAG 
TCTGCAGAGC TCCGCAGGAC TGAGTCAGAC AGTGTGTTGA AGAAGACGAG TAAGACCAAC 
CCTTTACTGC TGGTCAGGAG AAACAATGAG GTTCTTCAGA GCGTTGCTCA TCTTCATGAC 
CTGCTGAACA CATTACAAGC TGTGGTGGTC CAGCAGGACA CCTTCATCGA AGACCAGCGT 
CACGCTCATC CCTCCTCACG CCACCCATCC ATCTCCTCCT CTACCTCCTC TTTATCATCC 
ACCTCATCCC GGCCGAGCTC TCTAATCGAG CAGGAGAAAC AGCGCAGCCT GGAGAAGCAG 
CGGCAGGAGG TGGCCAGCCT CCAAAAACAG CAGGCGGCCC ACGCCGAGGA GAGGCGGCGC 
AGGGAAAAGC AGTGGGAGAC CCGAGAGAAA GAGCTAACCC AGAGGGAGAT GCAGATCCAG 
AGCCAAGAAG AGGAGGCGCA GAGGAGAGCC AGACAGCTGG AGGAGGAGAA ACAGGAGCTG 
CAGAGTAAGA AAGAGGAGTA TCAGAGAGAC CTGGCACGAC TGCGGGACAG CCAGAAGAGA 
CTGGAGAGAG AGAGAGAGCA GGTGCAGAGA GAGGCAGATG AGCTTAAACT GACTGAGGAA 
GTGACAAAGA ACCGGTCTCC GTCAACCACG TCTGAAGACT CCACGAAGTT CCAGAGCACC 
GGTTCATTAG ACCGTGACCC ATCAGAGATG GAGCTCTCCT CCTCCCCGTC AGCCAGAGAC 
TTCCTGTCCC GCATGGACTC CAAACGCAAA GGGAAAAACC TCAATCCATT CTCATCCAAC 
TCAAGCCAGA AGAGCCAGAA CAGCGAGGCT CAGAGCCAGA TGCCCAGCCG TCTGCTCCAG 
CTGGCCAAAC CCAAGGAGAA GAAGGACAAG AAGAAGAAGA AGGGGAAAGG CCAGCAGAGC 
CAGACTGCAG ACTCTCAGAG CACTGTTGTT CCAGGCGCTA CCCCAGACGG AGAGATCTTT 
TTCTGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

wu:fi15d04 ( XM_009297838.2 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_009296113.1 Danio rerio wu:fi15d04 (wu:fi15d04), transcript variant X6, mRNA.

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CloneID	ODa39932
Clone ID Related Accession (Same CDS sequence)	XM_009297838.2
Accession Version	XM_009297838.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7674bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-23
Organism	Danio rerio(Zebrafish)
Product	A-kinase anchor protein 13 isoform X6
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007136.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_009297838.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAGGGTG TTCCACAAGT GGCCAGTGCC ATGAAACTGA ATCCACAGCA AGCCCCATTA 
TATGGAGAGT GTCTGCTGAC GGTCCAGCTC TGTGAGGATG AGTGTGTTGA GGATGAGGAT 
GATGTGGAGT TTTACCTGCT CTTTGCTGGA TCCACACAGA GACATGTGTC CAGCACACTG 
AAGATCAGCC ATGTCACGCT GCAGGCCGTC TGTCCAGCTC ATGATCGCAG TGAAACGGTA 
CGGGTCACAC TATGCCAGGC TCGTCCCGGC GGCTCAGTGG ACCCAGTGTC CGAGGAGTGT 
TTCCAGTTTG TCCAGGACCT GGCTCTGGAC ATGGCCCAGT TCCTGGTAAA TGCGGCGGGG 
AAAACAGAAG GAGCGCTGCT GCTGGACGAG TGTCAGATCC CACTGAAAGA GTGCGAGCGG 
CTGGACGATA CTCTTGCTCT GGCTCTCCGG CATCTGCCTT TGCCTGCTGG ATGGAGCGTT 
TTGGGCACAG ATTTGGACAC GGGCACTTGC ACAGGCTCGG GCATCGCAGA GCCTGGACCA 
CACGATACAC TTTTGCACTT CGCTGCGAAG CGCGGACTAC GCAAGGTTGC ACTCTTTCTG 
CTCCAGCAAC CAGGGGGAAG AGAGGCTCTC CGGCTTCCCA ACAAACAGGG TCGCACACCG 
GCCAAGGTCG CCCAGAAGAG GGGACACTCT CAGCTCCACA AACTGCTCAC GGAGGTTGAC 
AATCTGCCCG AGCTGGAGAC GAAAACATCT AAGTGGTGTT ACCCAGAAGG CAGAGTCCTG 
CAACACCACC CCAAGCTAAA CACTTACACC CTTACCCTGG AGGATGTGCC CAGGAGCTCC 
CCACCAAGCC TTCAGTGTGA TGTGGAGGAG CTCCAGCGTC TCATTCAGTC ACATCAGAAG 
GAAGATCCTC CAACACCGCA TAAGTCAGCA CTGCTGATTC TCAATAGAGA CTCCAGTAAT 
AGCACAGAGA CAGATGACTG CCTTCCGCCC AACTCAGTGG AACCTGAGCA TCAGGAGCAC 
AACAAACAAG AAGAAACTTG CAAATCGCTT TTAAACAGCC TTACCTTTGA GGATGGATTG 
TGTGTTCATC AAAACGGCAC AGGAGATCAT GTCCAGTTGC TGGATGGGGA CGTGGAGCCT 
GACCTGCCAG CTGTAGGAGA TACAGGGAAT GCATTAGATA AGACTGACTG TTTGCAGCGG 
CGGGTAGATG GTGATTCTGT GGCTTTCAGT GCCCCTTCCT GTGGAAACCA GCGGGAGGAA 
GCCGATATAG AGCTCTGCAT AATCAGTGCC AAGCAGGGGG TTGCTTCACA GCCTGATAAA 
GGGAAGCAAG AAGAGCAAGA GGCTTGCCAG AAGAGGTCAG AGACTGAAAA GGACTATAAC 
ACTGAATCTG GCAACCTTGG GCTTCAAGAC ATGGGCCACG CACAATCTCA GGGTCTCCTC 
CCCACGGAAA CTGGTCAGCC TACACAAAGG GAGGTGGAGA GTGAGGGGAG TACAGATGTC 
TGTTGGCGTG GGGACTTACC AGAGGAAAGG GAAGGGGACA TGAGTAGCAA CCAGGAAATA 
GACTCATATT CTTCAGCACT TTCTTCCCAT AATCTAGAGG GAAACAGCCA GCACGATGGT 
GAGACTGAGC CCCAGTTGCC TACACAATGC TCAGAGGGAA CAGATGAAGC TAAATTGACT 
TTAATTCAGG TAAATTCTGA AGTTACTTTG TCTATACGAA GCGAGGATAC CGTTATTTCT 
GACAGTGTTG ACAAGCCACA TGGTCTCTTA GAGACGGAGA CAGGAAATCT CAGCGAACAC 
ACCAAAGGCA ACACTGAGTC AGCTGCTGAT TTGGTTGCGG ATGACGCTAG TGTAAAGCAG 
AACTCTGGAT TTAGCGTTTT AGAGGGGGAT CTTTTGGAAT CATTAAGTCT ACGGCCAAAC 
ATGGCACCGG GACCAGTGCA TAAGGTTCAG CAAGGTCTGT CACCAGTTCC TGAGACTTCC 
TCTCATGTAG GTCCAGCTTT GGACCCCTTT TCTGAGACTA CAGAAAGTTT TCAACAGCCG 
AATCAATCCA CACAGCTTTT AGAAACTGCA TTAGAATGTG AGGATGTTTC ATTAAATAAC 
TCAACCGGTG ACATTAATGC AATAGCAGGG GTTTCTGTTG ACATTTCTGC AGAGGTTTCA 
CTTTGCCAGA ATGAAGGGTG TCCTCCTGAT GGAAGCAAAG AAGAGGTAGC CCCCAGTGAG 
AGGATACCAG ACCTTAAAGA GCTTACGGAA GAGCCGGAAT CAGTTGAGGA CATTATTGTC 
TCAGATGTCT CAGATTTGGA TGACAAATCT CAACCTTTTG AAGAGTCTTC TTTGCCTATC 
GACTCTGAAT GTTTTATAAG AGGTGATTTC CAAAACGATG AGTCTACAGT ACAGTCTTCT 
GAATGTACTG GAGGTGACAT AAATGTAGGA ACACTCTTAG GAGAAGAAGA GGCAACTGAA 
AAAATAGAAC AGCCAGACTC TTCACTTAAA CTGGTTGAGC TAATTCCTGA GGAATATACT 
GGAGATGCCA CAAACGAAAA TAAAAAAATT GAGCCAGAGG AACAATCTAT TTTACTGCCA 
ACTTTGCAAG GGGAATCAGG ATTTAAGGAT GTTGCCACAG AGGAACCTGT ACAGGAAAGG 
GAAATTGACT CCACTTCAGA GGAAACAATC AGCGATCTCC CTGATCCATC GGAAATCACA 
AACAATGATT GTGCTGAATC CAATGCACAC TGCCAGCTCT CAGAATCCTC CAAGGAGAGC 
GCAAACACAT CCTCAGAACC TGCTGAATGT GTGAATGACC TAGACTTGTC TTGTGCTTTA 
CCTCAAACAG AAATCGAAGG CAGCAGACCT TCTGAGGGCC CCTTAATTGA TGGAGTAACA 
ATATGTGCTG AAATTCCCAT TGATGACAAA GGTGTTGACA TGGCTACCAA TATGAGTAAG 
ACGACCATCT CTGAGGATTG TCTGTCCTCT CCTATGGACA CCACCAGCTG TTCAGTTCTG 
GAGAGCTCCT CCATGTCTGA CCCACTCAAT GCCTCATCCT CAACAGCTGA GCTCACTTCC 
CCTTTGGATA TTAATAGTGG ATTGCATCAG GATTTGGAAC ACACTTCCCC AGTGGATATA 
GACAATGGTT TGCCTGAGGA ACCGCCGCCG TCAGACACAC CTGATTGCCT GAATGGATTA 
CATCAAGATT CTCCTGATAG TTTGATGGAG CACAGTGGAA CTCCTCAGCC ATGTTCTGAG 
GAACAGTGTG TGGATCAAAT CACAGATGAC AGCACTTCAG CAGAGGATGC AGCGGACGGA 
CACCAAGACT CCTCAGTAGA CATAGCAAAT TCCGAAGCGA TCCACAGAGA GACCGAAACA 
TTTTATTCAG TTGATGCCAG TCAAGACTCT GTACTCCAGG AGAAGAAATC TTCTTCAGTG 
AGCTCAGAAA TCCCCTCTTT GACCCCCTCA CCTGAGAGCA TACGTCACAA AGAGTCGCCA 
AGCACTATGC GAGGGTCCGC CAGCGATGGA GACTCTCTGT TTAGTCAGGA TCTGGGCGAG 
GACAGCGTTT TTAATAATAA GGCTGAGGAC AGCATTACCA TCACCAGTGG CGTGTCCATG 
TCCTATTCGT CCTCCACAGA TGACTCTTCC AGCTTGGGAA CCACTTGCGC CACCCCTCAG 
ACTGGCACAG AGGCTCCTGC ATCAGACCCC TTTCAGGAAC AGCCCACATC TACTGGAGCT 
TCTGGGGAAA CAGAGGAGGA AGAGAAAAAG GACCGTCTCA CTGAGGTTCC GGAGCGATCG 
GCCATTTTAC GGACCACCAT ACGATCACTG TCCCCTTTTC GAAGACACAG TTGGGGGCCG 
GGCAAAAATT TGGCAGGGGA GACAGAGATG AGTCAGCGCA GTGCGGCACA AGGTGAAGTG 
GAGCGCAGAT CTGTTGGTCA TAGGAGAAGC ATGAGCTGGT GTCCTTCTGT TGTCCTGCGC 
TCTGAAAATG ATGAAATGAA TGAAAGAAGT TACAGTCTGG AAGGACTTGC ACCAGAGAAG 
GAGGGAAGGA AAAGTCTGGT TCAGCAGCGT GTCGGAGAGT CCAGCCAGGA GCCCAAATGT 
CCATCAAAGG TGGAAAAAGA AGACAGAGGC TCGCTTGTGT CTCTGACTGA AGAAGAGCAG 
GAGTCAGATC TAGGCGACTG CAGCAGTTTG GGCAGTCAGA AGTCCATCCA GTCGGGTCGG 
AGATGTGTCC CACATTCACA ACCCATCCTC ACCAAATCTG TGTCCATGGT GGCCATCAGT 
CAGAAGGACC TGGACGCAAT GGGACGGCTG CGACCCAAGC GACGAATCTC CTTCTCCTTC 
AGCATCTCGC CGATGCTCCC CAAATCTAAA ACTCTCTTTT CTCTTGGCTC TTCATCCAGT 
GATGAGGAGG AGGCAGTCAG ATTGAGTTCA TTCACTGGAA CATCAGGATC ATTAGAGTAC 
AGTATATCAG AGGAGGATCC TGGGCCATTA CGGAGTGACA GTGAAGGCAA AATTGGAGGA 
ACCAAAGTGA GCAGGACATT CAGTTACCTC AAGAGCAAGA TGAGCAAGAA GAACAAGCAG 
GAGAAGGAGA AAGAGAAGAA TAAGGAGCGT GAGCGAGATG CCAAAGACAA GGAGAGAAGA 
GCCTCAAACG GACATCTCTT CACTACGGTC ATGACTGTAC CCACAATCCC CTGCCACCAG 
TGCAACAAAC TCATCAACAC TAAAGACTCT ATCCTCTGCA CCAATTGCAA TGCACAAGTC 
CACAAAGGCT GCAAAGAGAG TCTTCCTGTC TGCGCTAAAG TCAAGATGAA GCAGCAAAAA 
CAGCAGCAGA CTGTTACAGA TTCTGCCTCG ACGCCTAGTG TCACCCTCAG AAGTAAAACT 
CTTCCAGCAA GGGATCGTCC GTGGTCCACA ATATCCATTC CCGATGACCA GCCGGCTCCC 
CTCGCTCCTC CTCGGAAAAG CCCCAGCAGC ATCATGTCTT TCAACAGCAA CCCACTGTCC 
AAGAGCATGT CCATTAATAA CATAGCAGGA CAAATGGACG ACACTCCTCT TAAGAAGTTT 
CTGTCCCAGT CAACGGACTC TCTTCACAAG ACCAGTAAAG TCACGGAGTC TACAGAATCT 
CTTACTGATG AGGGTACAGA GATGATGGAC AGCCAGCTGA TGGGAGAATT TGAAGCTGAC 
GCCAAAGAGC TGGAGGCTGA TTCTTATAGC TTCACGGTAG ACAAGAAATT TCTTAAGCAG 
CTTAAAAAGG ATGTTATCAA AAGGCAAGAC GTCATCTATG AGCTCATCCA GACAGAAATG 
CACCATGTAA GGACCTTAAA GATCATGGCT GATGTTTACA GCAAAGGACT ACTGAGAGAA 
GTTCAACTAG AAGCTCAGAC GGTGGAGAAG ATGTTCCCCA TGTTGGACGA TGTTCTGGAT 
TTGCACACAC AGTTCTTTTC ACACCTGCTT GAAAGAAAGA AGGAATCAGG GAGCCAAGAA 
GAGGGCGGCT TTGTCATCCA AAGGATTGGC GATGTGCTGG TTTCTCAGTT CTCAGGTTCC 
AGTGCTGAGA GAATGAAGAA GGTCTATGGG AAGTTCTGCG GCAGGCACAA TGAAGCTGTG 
AACTTCTACA AGGAGCTTCA CACCAAAGAC AAACGCTTTC AAGCTTTCAT CAAGAAAAAG 
ATGAGCAGCC CTGTTGTCCG ACGGCTAAGC ATTCCTGAGT GTATATTGTT AGTCACACAA 
CGAATCACAA AATATCCCGT GTTACTACAG AGAATACTGC AGCACACGAA AGAAAACGAG 
GAGGACCATG CAGACGTTAG TCAAGGTCTG AAGCTGGTGA AGGAGGTGAT TGCTGCCGTG 
GACAATAAAG TGAATGAGCA TGAGAAGAAG AAGAGGCTAA AGGAAGTGTA CAGCCGGACG 
GACAACAAGT CCATCATGAG GATGAAGAGC GGGCAGATGT TTGCCAGAGA GGATCTGTTA 
CGTGGGCGGA AGTTAATTCA CGACGGGCCG CTGCAGCTCA AGAACTCAGC TGGCAGACTC 
AAGGATGTCC AGGCTCTGCT GCTGAGGGAC GTGATTGTGT TCCTGCAGGA GAAAGATCAG 
AAGTACATTT TCGCCTCTTT GGACCAGCGC TCCACCGTCA TCTCCCTGCA GAAGCTCATT 
GTGAGGGAAG TAGCCAATGA GGAGAAAGCT CTGTTTCTGA TCACAGCAGG CATCGAGAAG 
CCCGAGATGG TAGAAGTGTA CACAAGCTCC AAAGAGGAGA GAAACACATG GATGCAGCTC 
ATTCAGGAGG CCATGCAGTC TATGGAGAGA GATGATGATG AAGGTGTGCC AAGTGAAAAC 
GAAGAGGACA GGAGACTTCA GGAAATCAAG GTCAAGGAGT TCAGAGATCA GCTGCACAAA 
AGAGACGAGC AGATCTTGAC GTTACTGGAG GAGAAGGTGA AGTTGTTCAG GGACATGTGT 
GACTGTGGTG CACCGGACGA GACGGGGCTT CGTAATCGAA TGCTCTTCAG GGCCACGACG 
GATGATGTCA CCAGAGGAGA GCCAATCATG AAGGAGGCTC TGAAGGAAGT TGAAACGCTG 
CAGGTTCTGG TTAATGACAG TCTTGGCGGT GCGGTTCAGG AAGGTGTGTG CGCGGCTGGA 
CATGTGACTT TGCCACGACG AGCGGAAACA TTTGGAGGTT TCGACAGTCA CCAGATGAAC 
ATCTCTAAGA GCAAAGATGG AGACAGAGAG GAGCTGGAGG AGTCTGCAGA GCTCCGCAGG 
ACTGAGTCAG ACAGTGTGTT GAAGAAGACG AGTAAGACCA ACCCTTTACT GCTGGTCAGG 
AGAAACAATG AGCAGGTTCT TCAGAGCGTT GCTCATCTTC ATGACCTGCT GAACACATTA 
CAAGCTGTGG TGGTCCAGCA GGACACCTTC ATCGAAGACC AGCGTCACGC TCATCCCTCC 
TCACGCCACC CATCCATCTC CTCCTCTACC TCCTCTTTAT CATCCACCTC ATCCCGGCCG 
AGCTCTCTAA TCGAGCAGGA GAAACAGCGC AGCCTGGAGA AGCAGCGGCA GGAGGTGGCC 
AGCCTCCAAA AACAGCAGGC GGCCCACGCC GAGGAGAGGC GGCGCAGGGA AAAGCAGTGG 
GAGACCCGAG AGAAAGAGCT AACCCAGAGG GAGATGCAGA TCCAGAGCCA AGAAGAGGAG 
GCGCAGAGGA GAGCCAGACA GCTGGAGGAG GAGAAACAGG AGCTGCAGAG TAAGAAAGAG 
GAGTATCAGA GAGACCTGGC ACGACTGCGG GACAGCCAGA AGAGACTGGA GAGAGAGAGA 
GAGCAGGTGC AGAGAGAGGC AGATGAGCTT AAACTGACTG AGGAAGTGAC AAAGAACCGG 
TCTCCGTCAA CCACGTCTGA AGACTCCACG AAGTTCCAGA GCACCGGTTC ATTAGACCGT 
GACCCATCAG AGATGGAGCT CTCCTCCTCC CCGTCAGCCA GAGACTTCCT GTCCCGCATG 
GACTCCAAAC GCAAAGGGAA AAACCTCAAT CCATTCTCAT CCAACTCAAG CCAGAAGAGC 
CAGAACAGCG AGGCTCAGAG CCAGATGCCC AGCCGTCTGC TCCAGCTGGC CAAACCCAAG 
GAGAAGAAGG ACAAGAAGAA GAAGAAGGGG AAAGGCCAGC AGAGCCAGAC TGCAGACTCT 
CAGAGCACTG TTGTTCCAGG CGCTACCCCA GACGGAGAGA TCTTTTTCTG CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_009296113.1
CDS	28..7701
Translation	MKGVPQVASAMKLNPQQAPLYGECLLTVQLCEDECVEDEDDVEFYLLFAGSTQRHVSSTLKISHVTLQAVCPAHDRSETVRVTLCQARPGGSVDPVSEECFQFVQDLALDMAQFLVNAAGKTEGALLLDECQIPLKECERLDDTLALALRHLPLPAGWSVLGTDLDTGTCTGSGIAEPGPHDTLLHFAAKRGLRKVALFLLQQPGGREALRLPNKQGRTPAKVAQKRGHSQLHKLLTEVDNLPELETKTSKWCYPEGRVLQHHPKLNTYTLTLEDVPRSSPPSLQCDVEELQRLIQSHQKEDPPTPHKSALLILNRDSSNSTETDDCLPPNSVEPEHQEHNKQEETCKSLLNSLTFEDGLCVHQNGTGDHVQLLDGDVEPDLPAVGDTGNALDKTDCLQRRVDGDSVAFSAPSCGNQREEADIELCIISAKQGVASQPDKGKQEEQEACQKRSETEKDYNTESGNLGLQDMGHAQSQGLLPTETGQPTQREVESEGSTDVCWRGDLPEEREGDMSSNQEIDSYSSALSSHNLEGNSQHDGETEPQLPTQCSEGTDEAKLTLIQVNSEVTLSIRSEDTVISDSVDKPHGLLETETGNLSEHTKGNTESAADLVADDASVKQNSGFSVLEGDLLESLSLRPNMAPGPVHKVQQGLSPVPETSSHVGPALDPFSETTESFQQPNQSTQLLETALECEDVSLNNSTGDINAIAGVSVDISAEVSLCQNEGCPPDGSKEEVAPSERIPDLKELTEEPESVEDIIVSDVSDLDDKSQPFEESSLPIDSECFIRGDFQNDESTVQSSECTGGDINVGTLLGEEEATEKIEQPDSSLKLVELIPEEYTGDATNENKKIEPEEQSILLPTLQGESGFKDVATEEPVQEREIDSTSEETISDLPDPSEITNNDCAESNAHCQLSESSKESANTSSEPAECVNDLDLSCALPQTEIEGSRPSEGPLIDGVTICAEIPIDDKGVDMATNMSKTTISEDCLSSPMDTTSCSVLESSSMSDPLNASSSTAELTSPLDINSGLHQDLEHTSPVDIDNGLPEEPPPSDTPDCLNGLHQDSPDSLMEHSGTPQPCSEEQCVDQITDDSTSAEDAADGHQDSSVDIANSEAIHRETETFYSVDASQDSVLQEKKSSSVSSEIPSLTPSPESIRHKESPSTMRGSASDGDSLFSQDLGEDSVFNNKAEDSITITSGVSMSYSSSTDDSSSLGTTCATPQTGTEAPASDPFQEQPTSTGASGETEEEEKKDRLTEVPERSAILRTTIRSLSPFRRHSWGPGKNLAGETEMSQRSAAQGEVERRSVGHRRSMSWCPSVVLRSENDEMNERSYSLEGLAPEKEGRKSLVQQRVGESSQEPKCPSKVEKEDRGSLVSLTEEEQESDLGDCSSLGSQKSIQSGRRCVPHSQPILTKSVSMVAISQKDLDAMGRLRPKRRISFSFSISPMLPKSKTLFSLGSSSSDEEEAVRLSSFTGTSGSLEYSISEEDPGPLRSDSEGKIGGTKVSRTFSYLKSKMSKKNKQEKEKEKNKERERDAKDKERRASNGHLFTTVMTVPTIPCHQCNKLINTKDSILCTNCNAQVHKGCKESLPVCAKVKMKQQKQQQTVTDSASTPSVTLRSKTLPARDRPWSTISIPDDQPAPLAPPRKSPSSIMSFNSNPLSKSMSINNIAGQMDDTPLKKFLSQSTDSLHKTSKVTESTESLTDEGTEMMDSQLMGEFEADAKELEADSYSFTVDKKFLKQLKKDVIKRQDVIYELIQTEMHHVRTLKIMADVYSKGLLREVQLEAQTVEKMFPMLDDVLDLHTQFFSHLLERKKESGSQEEGGFVIQRIGDVLVSQFSGSSAERMKKVYGKFCGRHNEAVNFYKELHTKDKRFQAFIKKKMSSPVVRRLSIPECILLVTQRITKYPVLLQRILQHTKENEEDHADVSQGLKLVKEVIAAVDNKVNEHEKKKRLKEVYSRTDNKSIMRMKSGQMFAREDLLRGRKLIHDGPLQLKNSAGRLKDVQALLLRDVIVFLQEKDQKYIFASLDQRSTVISLQKLIVREVANEEKALFLITAGIEKPEMVEVYTSSKEERNTWMQLIQEAMQSMERDDDEGVPSENEEDRRLQEIKVKEFRDQLHKRDEQILTLLEEKVKLFRDMCDCGAPDETGLRNRMLFRATTDDVTRGEPIMKEALKEVETLQVLVNDSLGGAVQEGVCAAGHVTLPRRAETFGGFDSHQMNISKSKDGDREELEESAELRRTESDSVLKKTSKTNPLLLVRRNNEQVLQSVAHLHDLLNTLQAVVVQQDTFIEDQRHAHPSSRHPSISSSTSSLSSTSSRPSSLIEQEKQRSLEKQRQEVASLQKQQAAHAEERRRREKQWETREKELTQREMQIQSQEEEAQRRARQLEEEKQELQSKKEEYQRDLARLRDSQKRLEREREQVQREADELKLTEEVTKNRSPSTTSEDSTKFQSTGSLDRDPSEMELSSSPSARDFLSRMDSKRKGKNLNPFSSNSSQKSQNSEAQSQMPSRLLQLAKPKEKKDKKKKKGKGQQSQTADSQSTVVPGATPDGEIFFC

Target ORF information:

RefSeq Version	XM_009297838.2
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio wu:fi15d04 (wu:fi15d04), transcript variant X6, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_009297838.2

ORF Insert Sequence:

ATGAAGGGTG TTCCACAAGT GGCCAGTGCC ATGAAACTGA ATCCACAGCA AGCCCCATTA 
TATGGAGAGT GTCTGCTGAC GGTCCAGCTC TGTGAGGATG AGTGTGTTGA GGATGAGGAT 
GATGTGGAGT TTTACCTGCT CTTTGCTGGA TCCACACAGA GACATGTGTC CAGCACACTG 
AAGATCAGCC ATGTCACGCT GCAGGCCGTC TGTCCAGCTC ATGATCGCAG TGAAACGGTA 
CGGGTCACAC TATGCCAGGC TCGTCCCGGC GGCTCAGTGG ACCCAGTGTC CGAGGAGTGT 
TTCCAGTTTG TCCAGGACCT GGCTCTGGAC ATGGCCCAGT TCCTGGTAAA TGCGGCGGGG 
AAAACAGAAG GAGCGCTGCT GCTGGACGAG TGTCAGATCC CACTGAAAGA GTGCGAGCGG 
CTGGACGATA CTCTTGCTCT GGCTCTCCGG CATCTGCCTT TGCCTGCTGG ATGGAGCGTT 
TTGGGCACAG ATTTGGACAC GGGCACTTGC ACAGGCTCGG GCATCGCAGA GCCTGGACCA 
CACGATACAC TTTTGCACTT CGCTGCGAAG CGCGGACTAC GCAAGGTTGC ACTCTTTCTG 
CTCCAGCAAC CAGGGGGAAG AGAGGCTCTC CGGCTTCCCA ACAAACAGGG TCGCACACCG 
GCCAAGGTCG CCCAGAAGAG GGGACACTCT CAGCTCCACA AACTGCTCAC GGAGGTTGAC 
AATCTGCCCG AGCTGGAGAC GAAAACATCT AAGTGGTGTT ACCCAGAAGG CAGAGTCCTG 
CAACACCACC CCAAGCTAAA CACTTACACC CTTACCCTGG AGGATGTGCC CAGGAGCTCC 
CCACCAAGCC TTCAGTGTGA TGTGGAGGAG CTCCAGCGTC TCATTCAGTC ACATCAGAAG 
GAAGATCCTC CAACACCGCA TAAGTCAGCA CTGCTGATTC TCAATAGAGA CTCCAGTAAT 
AGCACAGAGA CAGATGACTG CCTTCCGCCC AACTCAGTGG AACCTGAGCA TCAGGAGCAC 
AACAAACAAG AAGAAACTTG CAAATCGCTT TTAAACAGCC TTACCTTTGA GGATGGATTG 
TGTGTTCATC AAAACGGCAC AGGAGATCAT GTCCAGTTGC TGGATGGGGA CGTGGAGCCT 
GACCTGCCAG CTGTAGGAGA TACAGGGAAT GCATTAGATA AGACTGACTG TTTGCAGCGG 
CGGGTAGATG GTGATTCTGT GGCTTTCAGT GCCCCTTCCT GTGGAAACCA GCGGGAGGAA 
GCCGATATAG AGCTCTGCAT AATCAGTGCC AAGCAGGGGG TTGCTTCACA GCCTGATAAA 
GGGAAGCAAG AAGAGCAAGA GGCTTGCCAG AAGAGGTCAG AGACTGAAAA GGACTATAAC 
ACTGAATCTG GCAACCTTGG GCTTCAAGAC ATGGGCCACG CACAATCTCA GGGTCTCCTC 
CCCACGGAAA CTGGTCAGCC TACACAAAGG GAGGTGGAGA GTGAGGGGAG TACAGATGTC 
TGTTGGCGTG GGGACTTACC AGAGGAAAGG GAAGGGGACA TGAGTAGCAA CCAGGAAATA 
GACTCATATT CTTCAGCACT TTCTTCCCAT AATCTAGAGG GAAACAGCCA GCACGATGGT 
GAGACTGAGC CCCAGTTGCC TACACAATGC TCAGAGGGAA CAGATGAAGC TAAATTGACT 
TTAATTCAGG TAAATTCTGA AGTTACTTTG TCTATACGAA GCGAGGATAC CGTTATTTCT 
GACAGTGTTG ACAAGCCACA TGGTCTCTTA GAGACGGAGA CAGGAAATCT CAGCGAACAC 
ACCAAAGGCA ACACTGAGTC AGCTGCTGAT TTGGTTGCGG ATGACGCTAG TGTAAAGCAG 
AACTCTGGAT TTAGCGTTTT AGAGGGGGAT CTTTTGGAAT CATTAAGTCT ACGGCCAAAC 
ATGGCACCGG GACCAGTGCA TAAGGTTCAG CAAGGTCTGT CACCAGTTCC TGAGACTTCC 
TCTCATGTAG GTCCAGCTTT GGACCCCTTT TCTGAGACTA CAGAAAGTTT TCAACAGCCG 
AATCAATCCA CACAGCTTTT AGAAACTGCA TTAGAATGTG AGGATGTTTC ATTAAATAAC 
TCAACCGGTG ACATTAATGC AATAGCAGGG GTTTCTGTTG ACATTTCTGC AGAGGTTTCA 
CTTTGCCAGA ATGAAGGGTG TCCTCCTGAT GGAAGCAAAG AAGAGGTAGC CCCCAGTGAG 
AGGATACCAG ACCTTAAAGA GCTTACGGAA GAGCCGGAAT CAGTTGAGGA CATTATTGTC 
TCAGATGTCT CAGATTTGGA TGACAAATCT CAACCTTTTG AAGAGTCTTC TTTGCCTATC 
GACTCTGAAT GTTTTATAAG AGGTGATTTC CAAAACGATG AGTCTACAGT ACAGTCTTCT 
GAATGTACTG GAGGTGACAT AAATGTAGGA ACACTCTTAG GAGAAGAAGA GGCAACTGAA 
AAAATAGAAC AGCCAGACTC TTCACTTAAA CTGGTTGAGC TAATTCCTGA GGAATATACT 
GGAGATGCCA CAAACGAAAA TAAAAAAATT GAGCCAGAGG AACAATCTAT TTTACTGCCA 
ACTTTGCAAG GGGAATCAGG ATTTAAGGAT GTTGCCACAG AGGAACCTGT ACAGGAAAGG 
GAAATTGACT CCACTTCAGA GGAAACAATC AGCGATCTCC CTGATCCATC GGAAATCACA 
AACAATGATT GTGCTGAATC CAATGCACAC TGCCAGCTCT CAGAATCCTC CAAGGAGAGC 
GCAAACACAT CCTCAGAACC TGCTGAATGT GTGAATGACC TAGACTTGTC TTGTGCTTTA 
CCTCAAACAG AAATCGAAGG CAGCAGACCT TCTGAGGGCC CCTTAATTGA TGGAGTAACA 
ATATGTGCTG AAATTCCCAT TGATGACAAA GGTGTTGACA TGGCTACCAA TATGAGTAAG 
ACGACCATCT CTGAGGATTG TCTGTCCTCT CCTATGGACA CCACCAGCTG TTCAGTTCTG 
GAGAGCTCCT CCATGTCTGA CCCACTCAAT GCCTCATCCT CAACAGCTGA GCTCACTTCC 
CCTTTGGATA TTAATAGTGG ATTGCATCAG GATTTGGAAC ACACTTCCCC AGTGGATATA 
GACAATGGTT TGCCTGAGGA ACCGCCGCCG TCAGACACAC CTGATTGCCT GAATGGATTA 
CATCAAGATT CTCCTGATAG TTTGATGGAG CACAGTGGAA CTCCTCAGCC ATGTTCTGAG 
GAACAGTGTG TGGATCAAAT CACAGATGAC AGCACTTCAG CAGAGGATGC AGCGGACGGA 
CACCAAGACT CCTCAGTAGA CATAGCAAAT TCCGAAGCGA TCCACAGAGA GACCGAAACA 
TTTTATTCAG TTGATGCCAG TCAAGACTCT GTACTCCAGG AGAAGAAATC TTCTTCAGTG 
AGCTCAGAAA TCCCCTCTTT GACCCCCTCA CCTGAGAGCA TACGTCACAA AGAGTCGCCA 
AGCACTATGC GAGGGTCCGC CAGCGATGGA GACTCTCTGT TTAGTCAGGA TCTGGGCGAG 
GACAGCGTTT TTAATAATAA GGCTGAGGAC AGCATTACCA TCACCAGTGG CGTGTCCATG 
TCCTATTCGT CCTCCACAGA TGACTCTTCC AGCTTGGGAA CCACTTGCGC CACCCCTCAG 
ACTGGCACAG AGGCTCCTGC ATCAGACCCC TTTCAGGAAC AGCCCACATC TACTGGAGCT 
TCTGGGGAAA CAGAGGAGGA AGAGAAAAAG GACCGTCTCA CTGAGGTTCC GGAGCGATCG 
GCCATTTTAC GGACCACCAT ACGATCACTG TCCCCTTTTC GAAGACACAG TTGGGGGCCG 
GGCAAAAATT TGGCAGGGGA GACAGAGATG AGTCAGCGCA GTGCGGCACA AGGTGAAGTG 
GAGCGCAGAT CTGTTGGTCA TAGGAGAAGC ATGAGCTGGT GTCCTTCTGT TGTCCTGCGC 
TCTGAAAATG ATGAAATGAA TGAAAGAAGT TACAGTCTGG AAGGACTTGC ACCAGAGAAG 
GAGGGAAGGA AAAGTCTGGT TCAGCAGCGT GTCGGAGAGT CCAGCCAGGA GCCCAAATGT 
CCATCAAAGG TGGAAAAAGA AGACAGAGGC TCGCTTGTGT CTCTGACTGA AGAAGAGCAG 
GAGTCAGATC TAGGCGACTG CAGCAGTTTG GGCAGTCAGA AGTCCATCCA GTCGGGTCGG 
AGATGTGTCC CACATTCACA ACCCATCCTC ACCAAATCTG TGTCCATGGT GGCCATCAGT 
CAGAAGGACC TGGACGCAAT GGGACGGCTG CGACCCAAGC GACGAATCTC CTTCTCCTTC 
AGCATCTCGC CGATGCTCCC CAAATCTAAA ACTCTCTTTT CTCTTGGCTC TTCATCCAGT 
GATGAGGAGG AGGCAGTCAG ATTGAGTTCA TTCACTGGAA CATCAGGATC ATTAGAGTAC 
AGTATATCAG AGGAGGATCC TGGGCCATTA CGGAGTGACA GTGAAGGCAA AATTGGAGGA 
ACCAAAGTGA GCAGGACATT CAGTTACCTC AAGAGCAAGA TGAGCAAGAA GAACAAGCAG 
GAGAAGGAGA AAGAGAAGAA TAAGGAGCGT GAGCGAGATG CCAAAGACAA GGAGAGAAGA 
GCCTCAAACG GACATCTCTT CACTACGGTC ATGACTGTAC CCACAATCCC CTGCCACCAG 
TGCAACAAAC TCATCAACAC TAAAGACTCT ATCCTCTGCA CCAATTGCAA TGCACAAGTC 
CACAAAGGCT GCAAAGAGAG TCTTCCTGTC TGCGCTAAAG TCAAGATGAA GCAGCAAAAA 
CAGCAGCAGA CTGTTACAGA TTCTGCCTCG ACGCCTAGTG TCACCCTCAG AAGTAAAACT 
CTTCCAGCAA GGGATCGTCC GTGGTCCACA ATATCCATTC CCGATGACCA GCCGGCTCCC 
CTCGCTCCTC CTCGGAAAAG CCCCAGCAGC ATCATGTCTT TCAACAGCAA CCCACTGTCC 
AAGAGCATGT CCATTAATAA CATAGCAGGA CAAATGGACG ACACTCCTCT TAAGAAGTTT 
CTGTCCCAGT CAACGGACTC TCTTCACAAG ACCAGTAAAG TCACGGAGTC TACAGAATCT 
CTTACTGATG AGGGTACAGA GATGATGGAC AGCCAGCTGA TGGGAGAATT TGAAGCTGAC 
GCCAAAGAGC TGGAGGCTGA TTCTTATAGC TTCACGGTAG ACAAGAAATT TCTTAAGCAG 
CTTAAAAAGG ATGTTATCAA AAGGCAAGAC GTCATCTATG AGCTCATCCA GACAGAAATG 
CACCATGTAA GGACCTTAAA GATCATGGCT GATGTTTACA GCAAAGGACT ACTGAGAGAA 
GTTCAACTAG AAGCTCAGAC GGTGGAGAAG ATGTTCCCCA TGTTGGACGA TGTTCTGGAT 
TTGCACACAC AGTTCTTTTC ACACCTGCTT GAAAGAAAGA AGGAATCAGG GAGCCAAGAA 
GAGGGCGGCT TTGTCATCCA AAGGATTGGC GATGTGCTGG TTTCTCAGTT CTCAGGTTCC 
AGTGCTGAGA GAATGAAGAA GGTCTATGGG AAGTTCTGCG GCAGGCACAA TGAAGCTGTG 
AACTTCTACA AGGAGCTTCA CACCAAAGAC AAACGCTTTC AAGCTTTCAT CAAGAAAAAG 
ATGAGCAGCC CTGTTGTCCG ACGGCTAAGC ATTCCTGAGT GTATATTGTT AGTCACACAA 
CGAATCACAA AATATCCCGT GTTACTACAG AGAATACTGC AGCACACGAA AGAAAACGAG 
GAGGACCATG CAGACGTTAG TCAAGGTCTG AAGCTGGTGA AGGAGGTGAT TGCTGCCGTG 
GACAATAAAG TGAATGAGCA TGAGAAGAAG AAGAGGCTAA AGGAAGTGTA CAGCCGGACG 
GACAACAAGT CCATCATGAG GATGAAGAGC GGGCAGATGT TTGCCAGAGA GGATCTGTTA 
CGTGGGCGGA AGTTAATTCA CGACGGGCCG CTGCAGCTCA AGAACTCAGC TGGCAGACTC 
AAGGATGTCC AGGCTCTGCT GCTGAGGGAC GTGATTGTGT TCCTGCAGGA GAAAGATCAG 
AAGTACATTT TCGCCTCTTT GGACCAGCGC TCCACCGTCA TCTCCCTGCA GAAGCTCATT 
GTGAGGGAAG TAGCCAATGA GGAGAAAGCT CTGTTTCTGA TCACAGCAGG CATCGAGAAG 
CCCGAGATGG TAGAAGTGTA CACAAGCTCC AAAGAGGAGA GAAACACATG GATGCAGCTC 
ATTCAGGAGG CCATGCAGTC TATGGAGAGA GATGATGATG AAGGTGTGCC AAGTGAAAAC 
GAAGAGGACA GGAGACTTCA GGAAATCAAG GTCAAGGAGT TCAGAGATCA GCTGCACAAA 
AGAGACGAGC AGATCTTGAC GTTACTGGAG GAGAAGGTGA AGTTGTTCAG GGACATGTGT 
GACTGTGGTG CACCGGACGA GACGGGGCTT CGTAATCGAA TGCTCTTCAG GGCCACGACG 
GATGATGTCA CCAGAGGAGA GCCAATCATG AAGGAGGCTC TGAAGGAAGT TGAAACGCTG 
CAGGTTCTGG TTAATGACAG TCTTGGCGGT GCGGTTCAGG AAGGTGTGTG CGCGGCTGGA 
CATGTGACTT TGCCACGACG AGCGGAAACA TTTGGAGGTT TCGACAGTCA CCAGATGAAC 
ATCTCTAAGA GCAAAGATGG AGACAGAGAG GAGCTGGAGG AGTCTGCAGA GCTCCGCAGG 
ACTGAGTCAG ACAGTGTGTT GAAGAAGACG AGTAAGACCA ACCCTTTACT GCTGGTCAGG 
AGAAACAATG AGCAGGTTCT TCAGAGCGTT GCTCATCTTC ATGACCTGCT GAACACATTA 
CAAGCTGTGG TGGTCCAGCA GGACACCTTC ATCGAAGACC AGCGTCACGC TCATCCCTCC 
TCACGCCACC CATCCATCTC CTCCTCTACC TCCTCTTTAT CATCCACCTC ATCCCGGCCG 
AGCTCTCTAA TCGAGCAGGA GAAACAGCGC AGCCTGGAGA AGCAGCGGCA GGAGGTGGCC 
AGCCTCCAAA AACAGCAGGC GGCCCACGCC GAGGAGAGGC GGCGCAGGGA AAAGCAGTGG 
GAGACCCGAG AGAAAGAGCT AACCCAGAGG GAGATGCAGA TCCAGAGCCA AGAAGAGGAG 
GCGCAGAGGA GAGCCAGACA GCTGGAGGAG GAGAAACAGG AGCTGCAGAG TAAGAAAGAG 
GAGTATCAGA GAGACCTGGC ACGACTGCGG GACAGCCAGA AGAGACTGGA GAGAGAGAGA 
GAGCAGGTGC AGAGAGAGGC AGATGAGCTT AAACTGACTG AGGAAGTGAC AAAGAACCGG 
TCTCCGTCAA CCACGTCTGA AGACTCCACG AAGTTCCAGA GCACCGGTTC ATTAGACCGT 
GACCCATCAG AGATGGAGCT CTCCTCCTCC CCGTCAGCCA GAGACTTCCT GTCCCGCATG 
GACTCCAAAC GCAAAGGGAA AAACCTCAAT CCATTCTCAT CCAACTCAAG CCAGAAGAGC 
CAGAACAGCG AGGCTCAGAG CCAGATGCCC AGCCGTCTGC TCCAGCTGGC CAAACCCAAG 
GAGAAGAAGG ACAAGAAGAA GAAGAAGGGG AAAGGCCAGC AGAGCCAGAC TGCAGACTCT 
CAGAGCACTG TTGTTCCAGG CGCTACCCCA GACGGAGAGA TCTTTTTCTG CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

wu:fi15d04 ( XM_009297839.2 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_009296114.1 Danio rerio wu:fi15d04 (wu:fi15d04), transcript variant X7, mRNA.

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CloneID	ODa39933
Clone ID Related Accession (Same CDS sequence)	XM_009297839.2 , XM_009297839.3
Accession Version	XM_009297839.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7662bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-23
Organism	Danio rerio(Zebrafish)
Product	A-kinase anchor protein 13 isoform X7
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007136.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_009297839.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAACTGA ATCCACAGCA AGCCCCATTA TATGGAGAGT GTCTGCTGAC GGTCCAGCTC 
TGTGAGGATG AGTGTGTTGA GGATGAGGAT GATGTGGAGT TTTACCTGCT CTTTGCTGGA 
TCCACACAGA GACATGTGTC CAGCACACTG AAGATCAGCC ATGTCACGCT GCAGGCCGTC 
TGTCCAGCTC ATGATCGCAG TGAAACGGTA CGGGTCACAC TATGCCAGGC TCGTCCCGGC 
GGCTCAGTGG ACCCAGTGTC CGAGGAGTGT TTCCAGTTTG TCCAGGACCT GGCTCTGGAC 
ATGGCCCAGT TCCTGGTAAA TGCGGCGGGG AAAACAGAAG GAGCGCTGCT GCTGGACGAG 
TGTCAGATCC CACTGAAAGA GTGCGAGCGG CTGGACGATA CTCTTGCTCT GGCTCTCCGG 
CATCTGCCTT TGCCTGCTGG ATGGAGCGTT TTGGGCACAG ATTTGGACAC GGGCACTTGC 
ACAGGCTCGG GCATCGCAGA GCCTGGACCA CACGATACAC TTTTGCACTT CGCTGCGAAG 
CGCGGACTAC GCAAGGTTGC ACTCTTTCTG CTCCAGCAAC CAGGGGGAAG AGAGGCTCTC 
CGGCTTCCCA ACAAACAGGG TCGCACACCG GCCAAGGTCG CCCAGAAGAG GGGACACTCT 
CAGCTCCACA AACTGCTCAC GGAGGTTGAC AATCTGCCCG AGCTGGAGAC GAAAACATCT 
AAGTGGTGTT ACCCAGAAGG CAGAGTCCTG CAACACCACC CCAAGCTAAA CACTTACACC 
CTTACCCTGG AGGATGTGCC CAGGAGCTCC CCACCAAGCC TTCAGTGTGA TGTGGAGGAG 
CTCCAGCGTC TCATTCAGTC ACATCAGAAG GAAGATCCTC CAACACCGCA TAAGTCAGCA 
CTGCTGATTC TCAATAGAGA CTCCAGTAAT AGCACAGAGA CAGATGACTG CCTTCCGCCC 
AACTCAGTGG AACCTGAGCA TCAGGAGCAC AACAAACAAG AAGAAACTTG CAAATCGCTT 
TTAAACAGCC TTACCTTTGA GGATGGATTG TGTGTTCATC AAAACGGCAC AGGAGATCAT 
GTCCAGTTGC TGGATGGGGA CGTGGAGCCT GACCTGCCAG CTGTAGGAGA TACAGGGAAT 
GCATTAGATA AGACTGACTG TTTGCAGCGG CGGGTAGATG GTGATTCTGT GGCTTTCAGT 
GCCCCTTCCT GTGGAAACCA GCGGGAGGAA GCCGATATAG AGCTCTGCAT AATCAGTGCC 
AAGCAGGGGG TTGCTTCACA GCCTGATAAA GGGAAGCAAG AAGAGCAAGA GGCTTGCCAG 
AAGAGGTCAG AGACTGAAAA GGACTATAAC ACTGAATCTG GCAACCTTGG GCTTCAAGAC 
ATGGGCCACG CACAATCTCA GGGTCTCCTC CCCACGGAAA CTGGTCAGCC TACACAAAGG 
GAGGTGGAGA GTGAGGGGAG TACAGATGTC TGTTGGCGTG GGGACTTACC AGAGGAAAGG 
GAAGGGGACA TGAGTAGCAA CCAGGAAATA GACTCATATT CTTCAGCACT TTCTTCCCAT 
AATCTAGAGG GAAACAGCCA GCACGATGGT GAGACTGAGC CCCAGTTGCC TACACAATGC 
TCAGAGGGAA CAGATGAAGC TAAATTGACT TTAATTCAGG TAAATTCTGA AGTTACTTTG 
TCTATACGAA GCGAGGATAC CGTTATTTCT GACAGTGTTG ACAAGCCACA TGGTCTCTTA 
GAGACGGAGA CAGGAAATCT CAGCGAACAC ACCAAAGGCA ACACTGAGTC AGCTGCTGAT 
TTGGTTGCGG ATGACGCTAG TGTAAAGCAG AACTCTGGAT TTAGCGTTTT AGAGGGGGAT 
CTTTTGGAAT CATTAAGTCT ACGGCCAAAC ATGGCACCGG GACCAGTGCA TAAGGTTCAG 
CAAGGTCTGT CACCAGTTCC TGAGACTTCC TCTCATGTAG GTCCAGCTTT GGACCCCTTT 
TCTGAGACTA CAGAAAGTTT TCAACAGCCG AATCAATCCA CACAGCTTTT AGAAACTGCA 
TTAGAATGTG AGGATGTTTC ATTAAATAAC TCAACCGGTG ACATTAATGC AATAGCAGGG 
GTTTCTGTTG ACATTTCTGC AGAGGTTTCA CTTTGCCAGA ATGAAGGGTG TCCTCCTGAT 
GGAAGCAAAG AAGAGGTAGC CCCCAGTGAG AGGATACCAG ACCTTAAAGA GCTTACGGAA 
GAGCCGGAAT CAGTTGAGGA CATTATTGTC TCAGATGTCT CAGATTTGGA TGACAAATCT 
CAACCTTTTG AAGAGTCTTC TTTGCCTATC GACTCTGAAT GTTTTATAAG AGGTGATTTC 
CAAAACGATG AGTCTACAGT ACAGTCTTCT GAATGTACTG GAGGTGACAT AAATGTAGGA 
ACACTCTTAG GAGAAGAAGA GGCAACTGAA AAAATAGAAC AGCCAGACTC TTCACTTAAA 
CTGGTTGAGC TAATTCCTGA GGAATATACT GGAGATGCCA CAAACGAAAA TAAAAAAATT 
GAGCCAGAGG AACAATCTAT TTTACTGCCA ACTTTGCAAG GGGAATCAGG ATTTAAGGAT 
GTTGCCACAG AGGAACCTGT ACAGGAAAGG GAAATTGACT CCACTTCAGA GGAAACAATC 
AGCGATCTCC CTGATCCATC GGAAATCACA AACAATGATT GTGCTGAATC CAATGCACAC 
TGCCAGCTCT CAGAATCCTC CAAGGAGAGC GCAAACACAT CCTCAGAACC TGCTGAATGT 
GTGAATGACC TAGACTTGTC TTGTGCTTTA CCTCAAACAG AAATCGAAGG CAGCAGACCT 
TCTGAGGGCC CCTTAATTGA TGGAGTAACA ATATGTGCTG AAATTCCCAT TGATGACAAA 
GGTGTTGACA TGGCTACCAA TATGAGTAAG ACGACCATCT CTGAGGATTG TCTGTCCTCT 
CCTATGGACA CCACCAGCTG TTCAGTTCTG GAGAGCTCCT CCATGTCTGA CCCACTCAAT 
GCCTCATCCT CAACAGCTGA GCTCACTTCC CCTTTGGATA TTAATAGTGG ATTGCATCAG 
GATTTGGAAC ACACTTCCCC AGTGGATATA GACAATGGTT TGCCTGAGGA ACCGCCGCCG 
TCAGACACAC CTGATTGCCT GAATGGATTA CATCAAGATT CTCCTGATAG TTTGATGGAG 
CACAGTGGAA CTCCTCAGCC ATGTTCTGAG GAACAGTGTG TGGATCAAAT CACAGATGAC 
AGCACTTCAG CAGAGGATGC AGCGGACGGA CACCAAGACT CCTCAGTAGA CATAGCAAAT 
TCCGAAGCGA TCCACAGAGA GACCGAAACA TTTTATTCAG TTGATGCCAG TCAAGACTCT 
GTACTCCAGG AGAAGAAATC TTCTTCAGTG AGCTCAGAAA TCCCCTCTTT GACCCCCTCA 
CCTGAGAGCA TACGTCACAA AGAGTCGCCA AGCACTATGC GAGGGTCCGC CAGCGATGGA 
GACTCTCTGT TTAGTCAGGA TCTGGGCGAG GACAGCGTTT TTAATAATAA GGCTGAGGAC 
AGCATTACCA TCACCAGTGG CGTGTCCATG TCCTATTCGT CCTCCACAGA TGACTCTTCC 
AGCTTGGGAA CCACTTGCGC CACCCCTCAG ACTGGCACAG AGGCTCCTGC ATCAGACCCC 
TTTCAGGAAC AGCCCACATC TACTGGAGCT TCTGGGGAAA CAGAGGAGGA AGAGAAAAAG 
GACCGTCTCA CTGAGGTTCC GGAGCGATCG GCCATTTTAC GGACCACCAT ACGATCACTG 
TCCCCTTTTC GAAGACACAG TTGGGGGCCG GGCAAAAATT TGGCAGGGGA GACAGAGATG 
AGTCAGCGCA GTGCGGCACA AGGTGAAGTG GAGCGCAGAT CTGTTGGTCA TAGGAGAAGC 
ATGAGCTGGT GTCCTTCTGT TGTCCTGCGC TCTGAAAATG ATGAAATGAA TGAAAGAAGT 
TACAGTCTGG AAGGACTTGC ACCAGAGAAG GAGGGAAGGA AAAGTCTGGT TCAGCAGCGT 
GTCGGAGAGT CCAGCCAGGA GCCCAAATGT CCATCAAAGG TGGAAAAAGA AGACAGAGGC 
TCGCTTGTGT CTCTGACTGA AGAAGAGCAG GAGTCAGATC TAGGCGACTG CAGCAGTTTG 
GGCAGTCAGA AGTCCATCCA GTCGGGTCGG AGATGTGTCC CACATTCACA ACCCATCCTC 
ACCAAATCTG TGTCCATGGT GGCCATCAGT CAGAAGGACC TGGACGCAAT GGGACGGCTG 
CGACCCAAGC GACGAATCTC CTTCTCCTTC AGCATCTCGC CGATGCTCCC CAAATCTAAA 
ACTCTCTTTT CTCTTGGCTC TTCATCCAGT GATGAGGAGG AGGCAGTCAG ATTGAGTTCA 
TTCACTGGAA CATCAGGATC ATTAGAGTAC AGTATATCAG AGGAGGATCC TGGGCCATTA 
CGGAGTGACA GTGAAGGCAA AATTGGAGGA ACCAAAGTGA GCAGGACATT CAGTTACCTC 
AAGAGCAAGA TGAGCAAGAA GAACAAGCAG GAGAAGGAGA AAGAGAAGAA TAAGGAGCGT 
GAGCGAGATG CCAAAGACAA GGAGAGAAGA GCCTCAAACG GACATCTCTT CACTACGGTC 
ATGACTGTAC CCACAATCCC CTGCCACCAG TGCAACAAAC TCATCAACAC TAAAGACTCT 
ATCCTCTGCA CCAATTGCAA TGCACAAGTC CACAAAGGCT GCAAAGAGAG TCTTCCTGTC 
TGCGCTAAAG TCAAGATGAA GCAGCAAAAA CAGCAGCAGA CTGTTACAGA TTCTGCCTCG 
ACGCCTAGTG TCACCCTCAG AAGTAAAACT CTTCCAGCAA GGGATCGTCC GTGGTCCACA 
ATATCCATTC CCGATGACCA GCCGGCTCCC CTCGCTCCTC CTCGGAAAAG CCCCAGCAGC 
ATCATGTCTT TCAACAGCAA CCCACTGTCC AAGAGCATGT CCATTAATAA CATAGCAGGA 
CAAATGGACG ACACTCCTCT TAAGAAGTTT CTGTCCCAGT CAACGGACTC TCTTCACAAG 
ACCAGTAAAG TCACGGAGTC TACAGAATCT CTTACTGATG AGGGTACAGA GATGATGGAC 
AGCCAGCTGA TGGGAGAATT TGAAGCTGAC GCCAAAGAGC TGGAGGCTGA TTCTTATAGC 
TTCACGGTAG ACAAGAAATT TCTTAAGCAG CTTAAAAAGG ATGTTATCAA AAGGCAAGAC 
GTCATCTATG AGCTCATCCA GACAGAAATG CACCATGTAA GGACCTTAAA GATCATGGCT 
GATGTTTACA GCAAAGGACT ACTGAGAGAA GTTCAACTAG AAGCTCAGAC GGTGGAGAAG 
ATGTTCCCCA TGTTGGACGA TGTTCTGGAT TTGCACACAC AGTTCTTTTC ACACCTGCTT 
GAAAGAAAGA AGGAATCAGG GAGCCAAGAA GAGGGCGGCT TTGTCATCCA AAGGATTGGC 
GATGTGCTGG TTTCTCAGTT CTCAGGTTCC AGTGCTGAGA GAATGAAGAA GGTCTATGGG 
AAGTTCTGCG GCAGGCACAA TGAAGCTGTG AACTTCTACA AGGAGCTTCA CACCAAAGAC 
AAACGCTTTC AAGCTTTCAT CAAGAAAAAG ATGAGCAGCC CTGTTGTCCG ACGGCTAAGC 
ATTCCTGAGT GTATATTGTT AGTCACACAA CGAATCACAA AATATCCCGT GTTACTACAG 
AGAATACTGC AGCACACGAA AGAAAACGAG GAGGACCATG CAGACGTTAG TCAAGGTCTG 
AAGCTGGTGA AGGAGGTGAT TGCTGCCGTG GACAATAAAG TGAATGAGCA TGAGAAGAAG 
AAGAGGCTAA AGGAAGTGTA CAGCCGGACG GACAACAAGT CCATCATGAG GATGAAGAGC 
GGGCAGATGT TTGCCAGAGA GGATCTGTTA CGTGGGCGGA AGTTAATTCA CGACGGGCCG 
CTGCAGCTCA AGAACTCAGC TGGCAGACTC AAGGATGTCC AGGCTCTGCT GCTGAGGGAC 
GTGATTGTGT TCCTGCAGGA GAAAGATCAG AAGTACATTT TCGCCTCTTT GGACCAGCGC 
TCCACCGTCA TCTCCCTGCA GAAGCTCATT GTGAGGGAAG TAGCCAATGA GGAGAAAGCT 
CTGTTTCTGA TCACAGCAGG CATCGAGAAG CCCGAGATGG TAGAAGTGTA CACAAGCTCC 
AAAGAGGAGA GAAACACATG GATGCAGCTC ATTCAGGAGG CCATGCAGTC TATACTGTCC 
ATTTCCTCCA GGGAGAGAGA TGATGATGAA GGTGTGCCAA GTGAAAACGA AGAGGACAGG 
AGACTTCAGG AAATCAAGGT CAAGGAGTTC AGAGATCAGC TGCACAAAAG AGACGAGCAG 
ATCTTGACGT TACTGGAGGA GAAGGTGAAG TTGTTCAGGG ACATGTGTGA CTGTGGTGCA 
CCGGACGAGA CGGGGCTTCG TAATCGAATG CTCTTCAGGG CCACGACGGA TGATGTCACC 
AGAGGAGAGC CAATCATGAA GGAGGCTCTG AAGGAAGTTG AAACGCTGCA GGTTCTGGTT 
AATGACAGTC TTGGCGGTGC GGTTCAGGAA GGTGTGTGCG CGGCTGGACA TGTGACTTTG 
CCACGACGAG CGGAAACATT TGGAGGTTTC GACAGTCACC AGATGAACAT CTCTAAGAGC 
AAAGATGGAG ACAGAGAGGA GCTGGAGGAG TCTGCAGAGC TCCGCAGGAC TGAGTCAGAC 
AGTGTGTTGA AGAAGACGAG TAAGACCAAC CCTTTACTGC TGGTCAGGAG AAACAATGAG 
CAGGTTCTTC AGAGCGTTGC TCATCTTCAT GACCTGCTGA ACACATTACA AGCTGTGGTG 
GTCCAGCAGG ACACCTTCAT CGAAGACCAG CGTCACGCTC ATCCCTCCTC ACGCCACCCA 
TCCATCTCCT CCTCTACCTC CTCTTTATCA TCCACCTCAT CCCGGCCGAG CTCTCTAATC 
GAGCAGGAGA AACAGCGCAG CCTGGAGAAG CAGCGGCAGG AGGTGGCCAG CCTCCAAAAA 
CAGCAGGCGG CCCACGCCGA GGAGAGGCGG CGCAGGGAAA AGCAGTGGGA GACCCGAGAG 
AAAGAGCTAA CCCAGAGGGA GATGCAGATC CAGAGCCAAG AAGAGGAGGC GCAGAGGAGA 
GCCAGACAGC TGGAGGAGGA GAAACAGGAG CTGCAGAGTA AGAAAGAGGA GTATCAGAGA 
GACCTGGCAC GACTGCGGGA CAGCCAGAAG AGACTGGAGA GAGAGAGAGA GCAGGTGCAG 
AGAGAGGCAG ATGAGCTTAA ACTGACTGAG GAAGTGACAA AGAACCGGTC TCCGTCAACC 
ACGTCTGAAG ACTCCACGAA GTTCCAGAGC ACCGGTTCAT TAGACCGTGA CCCATCAGAG 
ATGGAGCTCT CCTCCTCCCC GTCAGCCAGA GACTTCCTGT CCCGCATGGA CTCCAAACGC 
AAAGGGAAAA ACCTCAATCC ATTCTCATCC AACTCAAGCC AGAAGAGCCA GAACAGCGAG 
GCTCAGAGCC AGATGCCCAG CCGTCTGCTC CAGCTGGCCA AACCCAAGGA GAAGAAGGAC 
AAGAAGAAGA AGAAGGGGAA AGGCCAGCAG AGCCAGACTG CAGACTCTCA GAGCACTGTT 
GTTCCAGGCG CTACCCCAGA CGGAGAGATC TTTTTCTGCT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_009296114.1
CDS	390..8051
Translation	MKLNPQQAPLYGECLLTVQLCEDECVEDEDDVEFYLLFAGSTQRHVSSTLKISHVTLQAVCPAHDRSETVRVTLCQARPGGSVDPVSEECFQFVQDLALDMAQFLVNAAGKTEGALLLDECQIPLKECERLDDTLALALRHLPLPAGWSVLGTDLDTGTCTGSGIAEPGPHDTLLHFAAKRGLRKVALFLLQQPGGREALRLPNKQGRTPAKVAQKRGHSQLHKLLTEVDNLPELETKTSKWCYPEGRVLQHHPKLNTYTLTLEDVPRSSPPSLQCDVEELQRLIQSHQKEDPPTPHKSALLILNRDSSNSTETDDCLPPNSVEPEHQEHNKQEETCKSLLNSLTFEDGLCVHQNGTGDHVQLLDGDVEPDLPAVGDTGNALDKTDCLQRRVDGDSVAFSAPSCGNQREEADIELCIISAKQGVASQPDKGKQEEQEACQKRSETEKDYNTESGNLGLQDMGHAQSQGLLPTETGQPTQREVESEGSTDVCWRGDLPEEREGDMSSNQEIDSYSSALSSHNLEGNSQHDGETEPQLPTQCSEGTDEAKLTLIQVNSEVTLSIRSEDTVISDSVDKPHGLLETETGNLSEHTKGNTESAADLVADDASVKQNSGFSVLEGDLLESLSLRPNMAPGPVHKVQQGLSPVPETSSHVGPALDPFSETTESFQQPNQSTQLLETALECEDVSLNNSTGDINAIAGVSVDISAEVSLCQNEGCPPDGSKEEVAPSERIPDLKELTEEPESVEDIIVSDVSDLDDKSQPFEESSLPIDSECFIRGDFQNDESTVQSSECTGGDINVGTLLGEEEATEKIEQPDSSLKLVELIPEEYTGDATNENKKIEPEEQSILLPTLQGESGFKDVATEEPVQEREIDSTSEETISDLPDPSEITNNDCAESNAHCQLSESSKESANTSSEPAECVNDLDLSCALPQTEIEGSRPSEGPLIDGVTICAEIPIDDKGVDMATNMSKTTISEDCLSSPMDTTSCSVLESSSMSDPLNASSSTAELTSPLDINSGLHQDLEHTSPVDIDNGLPEEPPPSDTPDCLNGLHQDSPDSLMEHSGTPQPCSEEQCVDQITDDSTSAEDAADGHQDSSVDIANSEAIHRETETFYSVDASQDSVLQEKKSSSVSSEIPSLTPSPESIRHKESPSTMRGSASDGDSLFSQDLGEDSVFNNKAEDSITITSGVSMSYSSSTDDSSSLGTTCATPQTGTEAPASDPFQEQPTSTGASGETEEEEKKDRLTEVPERSAILRTTIRSLSPFRRHSWGPGKNLAGETEMSQRSAAQGEVERRSVGHRRSMSWCPSVVLRSENDEMNERSYSLEGLAPEKEGRKSLVQQRVGESSQEPKCPSKVEKEDRGSLVSLTEEEQESDLGDCSSLGSQKSIQSGRRCVPHSQPILTKSVSMVAISQKDLDAMGRLRPKRRISFSFSISPMLPKSKTLFSLGSSSSDEEEAVRLSSFTGTSGSLEYSISEEDPGPLRSDSEGKIGGTKVSRTFSYLKSKMSKKNKQEKEKEKNKERERDAKDKERRASNGHLFTTVMTVPTIPCHQCNKLINTKDSILCTNCNAQVHKGCKESLPVCAKVKMKQQKQQQTVTDSASTPSVTLRSKTLPARDRPWSTISIPDDQPAPLAPPRKSPSSIMSFNSNPLSKSMSINNIAGQMDDTPLKKFLSQSTDSLHKTSKVTESTESLTDEGTEMMDSQLMGEFEADAKELEADSYSFTVDKKFLKQLKKDVIKRQDVIYELIQTEMHHVRTLKIMADVYSKGLLREVQLEAQTVEKMFPMLDDVLDLHTQFFSHLLERKKESGSQEEGGFVIQRIGDVLVSQFSGSSAERMKKVYGKFCGRHNEAVNFYKELHTKDKRFQAFIKKKMSSPVVRRLSIPECILLVTQRITKYPVLLQRILQHTKENEEDHADVSQGLKLVKEVIAAVDNKVNEHEKKKRLKEVYSRTDNKSIMRMKSGQMFAREDLLRGRKLIHDGPLQLKNSAGRLKDVQALLLRDVIVFLQEKDQKYIFASLDQRSTVISLQKLIVREVANEEKALFLITAGIEKPEMVEVYTSSKEERNTWMQLIQEAMQSILSISSRERDDDEGVPSENEEDRRLQEIKVKEFRDQLHKRDEQILTLLEEKVKLFRDMCDCGAPDETGLRNRMLFRATTDDVTRGEPIMKEALKEVETLQVLVNDSLGGAVQEGVCAAGHVTLPRRAETFGGFDSHQMNISKSKDGDREELEESAELRRTESDSVLKKTSKTNPLLLVRRNNEQVLQSVAHLHDLLNTLQAVVVQQDTFIEDQRHAHPSSRHPSISSSTSSLSSTSSRPSSLIEQEKQRSLEKQRQEVASLQKQQAAHAEERRRREKQWETREKELTQREMQIQSQEEEAQRRARQLEEEKQELQSKKEEYQRDLARLRDSQKRLEREREQVQREADELKLTEEVTKNRSPSTTSEDSTKFQSTGSLDRDPSEMELSSSPSARDFLSRMDSKRKGKNLNPFSSNSSQKSQNSEAQSQMPSRLLQLAKPKEKKDKKKKKGKGQQSQTADSQSTVVPGATPDGEIFFC

Target ORF information:

RefSeq Version	XM_009297839.2
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio wu:fi15d04 (wu:fi15d04), transcript variant X7, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_009297839.2

ORF Insert Sequence:

ATGAAACTGA ATCCACAGCA AGCCCCATTA TATGGAGAGT GTCTGCTGAC GGTCCAGCTC 
TGTGAGGATG AGTGTGTTGA GGATGAGGAT GATGTGGAGT TTTACCTGCT CTTTGCTGGA 
TCCACACAGA GACATGTGTC CAGCACACTG AAGATCAGCC ATGTCACGCT GCAGGCCGTC 
TGTCCAGCTC ATGATCGCAG TGAAACGGTA CGGGTCACAC TATGCCAGGC TCGTCCCGGC 
GGCTCAGTGG ACCCAGTGTC CGAGGAGTGT TTCCAGTTTG TCCAGGACCT GGCTCTGGAC 
ATGGCCCAGT TCCTGGTAAA TGCGGCGGGG AAAACAGAAG GAGCGCTGCT GCTGGACGAG 
TGTCAGATCC CACTGAAAGA GTGCGAGCGG CTGGACGATA CTCTTGCTCT GGCTCTCCGG 
CATCTGCCTT TGCCTGCTGG ATGGAGCGTT TTGGGCACAG ATTTGGACAC GGGCACTTGC 
ACAGGCTCGG GCATCGCAGA GCCTGGACCA CACGATACAC TTTTGCACTT CGCTGCGAAG 
CGCGGACTAC GCAAGGTTGC ACTCTTTCTG CTCCAGCAAC CAGGGGGAAG AGAGGCTCTC 
CGGCTTCCCA ACAAACAGGG TCGCACACCG GCCAAGGTCG CCCAGAAGAG GGGACACTCT 
CAGCTCCACA AACTGCTCAC GGAGGTTGAC AATCTGCCCG AGCTGGAGAC GAAAACATCT 
AAGTGGTGTT ACCCAGAAGG CAGAGTCCTG CAACACCACC CCAAGCTAAA CACTTACACC 
CTTACCCTGG AGGATGTGCC CAGGAGCTCC CCACCAAGCC TTCAGTGTGA TGTGGAGGAG 
CTCCAGCGTC TCATTCAGTC ACATCAGAAG GAAGATCCTC CAACACCGCA TAAGTCAGCA 
CTGCTGATTC TCAATAGAGA CTCCAGTAAT AGCACAGAGA CAGATGACTG CCTTCCGCCC 
AACTCAGTGG AACCTGAGCA TCAGGAGCAC AACAAACAAG AAGAAACTTG CAAATCGCTT 
TTAAACAGCC TTACCTTTGA GGATGGATTG TGTGTTCATC AAAACGGCAC AGGAGATCAT 
GTCCAGTTGC TGGATGGGGA CGTGGAGCCT GACCTGCCAG CTGTAGGAGA TACAGGGAAT 
GCATTAGATA AGACTGACTG TTTGCAGCGG CGGGTAGATG GTGATTCTGT GGCTTTCAGT 
GCCCCTTCCT GTGGAAACCA GCGGGAGGAA GCCGATATAG AGCTCTGCAT AATCAGTGCC 
AAGCAGGGGG TTGCTTCACA GCCTGATAAA GGGAAGCAAG AAGAGCAAGA GGCTTGCCAG 
AAGAGGTCAG AGACTGAAAA GGACTATAAC ACTGAATCTG GCAACCTTGG GCTTCAAGAC 
ATGGGCCACG CACAATCTCA GGGTCTCCTC CCCACGGAAA CTGGTCAGCC TACACAAAGG 
GAGGTGGAGA GTGAGGGGAG TACAGATGTC TGTTGGCGTG GGGACTTACC AGAGGAAAGG 
GAAGGGGACA TGAGTAGCAA CCAGGAAATA GACTCATATT CTTCAGCACT TTCTTCCCAT 
AATCTAGAGG GAAACAGCCA GCACGATGGT GAGACTGAGC CCCAGTTGCC TACACAATGC 
TCAGAGGGAA CAGATGAAGC TAAATTGACT TTAATTCAGG TAAATTCTGA AGTTACTTTG 
TCTATACGAA GCGAGGATAC CGTTATTTCT GACAGTGTTG ACAAGCCACA TGGTCTCTTA 
GAGACGGAGA CAGGAAATCT CAGCGAACAC ACCAAAGGCA ACACTGAGTC AGCTGCTGAT 
TTGGTTGCGG ATGACGCTAG TGTAAAGCAG AACTCTGGAT TTAGCGTTTT AGAGGGGGAT 
CTTTTGGAAT CATTAAGTCT ACGGCCAAAC ATGGCACCGG GACCAGTGCA TAAGGTTCAG 
CAAGGTCTGT CACCAGTTCC TGAGACTTCC TCTCATGTAG GTCCAGCTTT GGACCCCTTT 
TCTGAGACTA CAGAAAGTTT TCAACAGCCG AATCAATCCA CACAGCTTTT AGAAACTGCA 
TTAGAATGTG AGGATGTTTC ATTAAATAAC TCAACCGGTG ACATTAATGC AATAGCAGGG 
GTTTCTGTTG ACATTTCTGC AGAGGTTTCA CTTTGCCAGA ATGAAGGGTG TCCTCCTGAT 
GGAAGCAAAG AAGAGGTAGC CCCCAGTGAG AGGATACCAG ACCTTAAAGA GCTTACGGAA 
GAGCCGGAAT CAGTTGAGGA CATTATTGTC TCAGATGTCT CAGATTTGGA TGACAAATCT 
CAACCTTTTG AAGAGTCTTC TTTGCCTATC GACTCTGAAT GTTTTATAAG AGGTGATTTC 
CAAAACGATG AGTCTACAGT ACAGTCTTCT GAATGTACTG GAGGTGACAT AAATGTAGGA 
ACACTCTTAG GAGAAGAAGA GGCAACTGAA AAAATAGAAC AGCCAGACTC TTCACTTAAA 
CTGGTTGAGC TAATTCCTGA GGAATATACT GGAGATGCCA CAAACGAAAA TAAAAAAATT 
GAGCCAGAGG AACAATCTAT TTTACTGCCA ACTTTGCAAG GGGAATCAGG ATTTAAGGAT 
GTTGCCACAG AGGAACCTGT ACAGGAAAGG GAAATTGACT CCACTTCAGA GGAAACAATC 
AGCGATCTCC CTGATCCATC GGAAATCACA AACAATGATT GTGCTGAATC CAATGCACAC 
TGCCAGCTCT CAGAATCCTC CAAGGAGAGC GCAAACACAT CCTCAGAACC TGCTGAATGT 
GTGAATGACC TAGACTTGTC TTGTGCTTTA CCTCAAACAG AAATCGAAGG CAGCAGACCT 
TCTGAGGGCC CCTTAATTGA TGGAGTAACA ATATGTGCTG AAATTCCCAT TGATGACAAA 
GGTGTTGACA TGGCTACCAA TATGAGTAAG ACGACCATCT CTGAGGATTG TCTGTCCTCT 
CCTATGGACA CCACCAGCTG TTCAGTTCTG GAGAGCTCCT CCATGTCTGA CCCACTCAAT 
GCCTCATCCT CAACAGCTGA GCTCACTTCC CCTTTGGATA TTAATAGTGG ATTGCATCAG 
GATTTGGAAC ACACTTCCCC AGTGGATATA GACAATGGTT TGCCTGAGGA ACCGCCGCCG 
TCAGACACAC CTGATTGCCT GAATGGATTA CATCAAGATT CTCCTGATAG TTTGATGGAG 
CACAGTGGAA CTCCTCAGCC ATGTTCTGAG GAACAGTGTG TGGATCAAAT CACAGATGAC 
AGCACTTCAG CAGAGGATGC AGCGGACGGA CACCAAGACT CCTCAGTAGA CATAGCAAAT 
TCCGAAGCGA TCCACAGAGA GACCGAAACA TTTTATTCAG TTGATGCCAG TCAAGACTCT 
GTACTCCAGG AGAAGAAATC TTCTTCAGTG AGCTCAGAAA TCCCCTCTTT GACCCCCTCA 
CCTGAGAGCA TACGTCACAA AGAGTCGCCA AGCACTATGC GAGGGTCCGC CAGCGATGGA 
GACTCTCTGT TTAGTCAGGA TCTGGGCGAG GACAGCGTTT TTAATAATAA GGCTGAGGAC 
AGCATTACCA TCACCAGTGG CGTGTCCATG TCCTATTCGT CCTCCACAGA TGACTCTTCC 
AGCTTGGGAA CCACTTGCGC CACCCCTCAG ACTGGCACAG AGGCTCCTGC ATCAGACCCC 
TTTCAGGAAC AGCCCACATC TACTGGAGCT TCTGGGGAAA CAGAGGAGGA AGAGAAAAAG 
GACCGTCTCA CTGAGGTTCC GGAGCGATCG GCCATTTTAC GGACCACCAT ACGATCACTG 
TCCCCTTTTC GAAGACACAG TTGGGGGCCG GGCAAAAATT TGGCAGGGGA GACAGAGATG 
AGTCAGCGCA GTGCGGCACA AGGTGAAGTG GAGCGCAGAT CTGTTGGTCA TAGGAGAAGC 
ATGAGCTGGT GTCCTTCTGT TGTCCTGCGC TCTGAAAATG ATGAAATGAA TGAAAGAAGT 
TACAGTCTGG AAGGACTTGC ACCAGAGAAG GAGGGAAGGA AAAGTCTGGT TCAGCAGCGT 
GTCGGAGAGT CCAGCCAGGA GCCCAAATGT CCATCAAAGG TGGAAAAAGA AGACAGAGGC 
TCGCTTGTGT CTCTGACTGA AGAAGAGCAG GAGTCAGATC TAGGCGACTG CAGCAGTTTG 
GGCAGTCAGA AGTCCATCCA GTCGGGTCGG AGATGTGTCC CACATTCACA ACCCATCCTC 
ACCAAATCTG TGTCCATGGT GGCCATCAGT CAGAAGGACC TGGACGCAAT GGGACGGCTG 
CGACCCAAGC GACGAATCTC CTTCTCCTTC AGCATCTCGC CGATGCTCCC CAAATCTAAA 
ACTCTCTTTT CTCTTGGCTC TTCATCCAGT GATGAGGAGG AGGCAGTCAG ATTGAGTTCA 
TTCACTGGAA CATCAGGATC ATTAGAGTAC AGTATATCAG AGGAGGATCC TGGGCCATTA 
CGGAGTGACA GTGAAGGCAA AATTGGAGGA ACCAAAGTGA GCAGGACATT CAGTTACCTC 
AAGAGCAAGA TGAGCAAGAA GAACAAGCAG GAGAAGGAGA AAGAGAAGAA TAAGGAGCGT 
GAGCGAGATG CCAAAGACAA GGAGAGAAGA GCCTCAAACG GACATCTCTT CACTACGGTC 
ATGACTGTAC CCACAATCCC CTGCCACCAG TGCAACAAAC TCATCAACAC TAAAGACTCT 
ATCCTCTGCA CCAATTGCAA TGCACAAGTC CACAAAGGCT GCAAAGAGAG TCTTCCTGTC 
TGCGCTAAAG TCAAGATGAA GCAGCAAAAA CAGCAGCAGA CTGTTACAGA TTCTGCCTCG 
ACGCCTAGTG TCACCCTCAG AAGTAAAACT CTTCCAGCAA GGGATCGTCC GTGGTCCACA 
ATATCCATTC CCGATGACCA GCCGGCTCCC CTCGCTCCTC CTCGGAAAAG CCCCAGCAGC 
ATCATGTCTT TCAACAGCAA CCCACTGTCC AAGAGCATGT CCATTAATAA CATAGCAGGA 
CAAATGGACG ACACTCCTCT TAAGAAGTTT CTGTCCCAGT CAACGGACTC TCTTCACAAG 
ACCAGTAAAG TCACGGAGTC TACAGAATCT CTTACTGATG AGGGTACAGA GATGATGGAC 
AGCCAGCTGA TGGGAGAATT TGAAGCTGAC GCCAAAGAGC TGGAGGCTGA TTCTTATAGC 
TTCACGGTAG ACAAGAAATT TCTTAAGCAG CTTAAAAAGG ATGTTATCAA AAGGCAAGAC 
GTCATCTATG AGCTCATCCA GACAGAAATG CACCATGTAA GGACCTTAAA GATCATGGCT 
GATGTTTACA GCAAAGGACT ACTGAGAGAA GTTCAACTAG AAGCTCAGAC GGTGGAGAAG 
ATGTTCCCCA TGTTGGACGA TGTTCTGGAT TTGCACACAC AGTTCTTTTC ACACCTGCTT 
GAAAGAAAGA AGGAATCAGG GAGCCAAGAA GAGGGCGGCT TTGTCATCCA AAGGATTGGC 
GATGTGCTGG TTTCTCAGTT CTCAGGTTCC AGTGCTGAGA GAATGAAGAA GGTCTATGGG 
AAGTTCTGCG GCAGGCACAA TGAAGCTGTG AACTTCTACA AGGAGCTTCA CACCAAAGAC 
AAACGCTTTC AAGCTTTCAT CAAGAAAAAG ATGAGCAGCC CTGTTGTCCG ACGGCTAAGC 
ATTCCTGAGT GTATATTGTT AGTCACACAA CGAATCACAA AATATCCCGT GTTACTACAG 
AGAATACTGC AGCACACGAA AGAAAACGAG GAGGACCATG CAGACGTTAG TCAAGGTCTG 
AAGCTGGTGA AGGAGGTGAT TGCTGCCGTG GACAATAAAG TGAATGAGCA TGAGAAGAAG 
AAGAGGCTAA AGGAAGTGTA CAGCCGGACG GACAACAAGT CCATCATGAG GATGAAGAGC 
GGGCAGATGT TTGCCAGAGA GGATCTGTTA CGTGGGCGGA AGTTAATTCA CGACGGGCCG 
CTGCAGCTCA AGAACTCAGC TGGCAGACTC AAGGATGTCC AGGCTCTGCT GCTGAGGGAC 
GTGATTGTGT TCCTGCAGGA GAAAGATCAG AAGTACATTT TCGCCTCTTT GGACCAGCGC 
TCCACCGTCA TCTCCCTGCA GAAGCTCATT GTGAGGGAAG TAGCCAATGA GGAGAAAGCT 
CTGTTTCTGA TCACAGCAGG CATCGAGAAG CCCGAGATGG TAGAAGTGTA CACAAGCTCC 
AAAGAGGAGA GAAACACATG GATGCAGCTC ATTCAGGAGG CCATGCAGTC TATACTGTCC 
ATTTCCTCCA GGGAGAGAGA TGATGATGAA GGTGTGCCAA GTGAAAACGA AGAGGACAGG 
AGACTTCAGG AAATCAAGGT CAAGGAGTTC AGAGATCAGC TGCACAAAAG AGACGAGCAG 
ATCTTGACGT TACTGGAGGA GAAGGTGAAG TTGTTCAGGG ACATGTGTGA CTGTGGTGCA 
CCGGACGAGA CGGGGCTTCG TAATCGAATG CTCTTCAGGG CCACGACGGA TGATGTCACC 
AGAGGAGAGC CAATCATGAA GGAGGCTCTG AAGGAAGTTG AAACGCTGCA GGTTCTGGTT 
AATGACAGTC TTGGCGGTGC GGTTCAGGAA GGTGTGTGCG CGGCTGGACA TGTGACTTTG 
CCACGACGAG CGGAAACATT TGGAGGTTTC GACAGTCACC AGATGAACAT CTCTAAGAGC 
AAAGATGGAG ACAGAGAGGA GCTGGAGGAG TCTGCAGAGC TCCGCAGGAC TGAGTCAGAC 
AGTGTGTTGA AGAAGACGAG TAAGACCAAC CCTTTACTGC TGGTCAGGAG AAACAATGAG 
CAGGTTCTTC AGAGCGTTGC TCATCTTCAT GACCTGCTGA ACACATTACA AGCTGTGGTG 
GTCCAGCAGG ACACCTTCAT CGAAGACCAG CGTCACGCTC ATCCCTCCTC ACGCCACCCA 
TCCATCTCCT CCTCTACCTC CTCTTTATCA TCCACCTCAT CCCGGCCGAG CTCTCTAATC 
GAGCAGGAGA AACAGCGCAG CCTGGAGAAG CAGCGGCAGG AGGTGGCCAG CCTCCAAAAA 
CAGCAGGCGG CCCACGCCGA GGAGAGGCGG CGCAGGGAAA AGCAGTGGGA GACCCGAGAG 
AAAGAGCTAA CCCAGAGGGA GATGCAGATC CAGAGCCAAG AAGAGGAGGC GCAGAGGAGA 
GCCAGACAGC TGGAGGAGGA GAAACAGGAG CTGCAGAGTA AGAAAGAGGA GTATCAGAGA 
GACCTGGCAC GACTGCGGGA CAGCCAGAAG AGACTGGAGA GAGAGAGAGA GCAGGTGCAG 
AGAGAGGCAG ATGAGCTTAA ACTGACTGAG GAAGTGACAA AGAACCGGTC TCCGTCAACC 
ACGTCTGAAG ACTCCACGAA GTTCCAGAGC ACCGGTTCAT TAGACCGTGA CCCATCAGAG 
ATGGAGCTCT CCTCCTCCCC GTCAGCCAGA GACTTCCTGT CCCGCATGGA CTCCAAACGC 
AAAGGGAAAA ACCTCAATCC ATTCTCATCC AACTCAAGCC AGAAGAGCCA GAACAGCGAG 
GCTCAGAGCC AGATGCCCAG CCGTCTGCTC CAGCTGGCCA AACCCAAGGA GAAGAAGGAC 
AAGAAGAAGA AGAAGGGGAA AGGCCAGCAG AGCCAGACTG CAGACTCTCA GAGCACTGTT 
GTTCCAGGCG CTACCCCAGA CGGAGAGATC TTTTTCTGCT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

wu:fi15d04 ( XM_009297839.3 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_009296114.1 Danio rerio wu:fi15d04 (wu:fi15d04), transcript variant X1, mRNA.

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CloneID	ODa39933
Clone ID Related Accession (Same CDS sequence)	XM_009297839.2 , XM_009297839.3
Accession Version	XM_009297839.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7662bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	A-kinase anchor protein 13 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007136.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_009297839.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAACTGA ATCCACAGCA AGCCCCATTA TATGGAGAGT GTCTGCTGAC GGTCCAGCTC 
TGTGAGGATG AGTGTGTTGA GGATGAGGAT GATGTGGAGT TTTACCTGCT CTTTGCTGGA 
TCCACACAGA GACATGTGTC CAGCACACTG AAGATCAGCC ATGTCACGCT GCAGGCCGTC 
TGTCCAGCTC ATGATCGCAG TGAAACGGTA CGGGTCACAC TATGCCAGGC TCGTCCCGGC 
GGCTCAGTGG ACCCAGTGTC CGAGGAGTGT TTCCAGTTTG TCCAGGACCT GGCTCTGGAC 
ATGGCCCAGT TCCTGGTAAA TGCGGCGGGG AAAACAGAAG GAGCGCTGCT GCTGGACGAG 
TGTCAGATCC CACTGAAAGA GTGCGAGCGG CTGGACGATA CTCTTGCTCT GGCTCTCCGG 
CATCTGCCTT TGCCTGCTGG ATGGAGCGTT TTGGGCACAG ATTTGGACAC GGGCACTTGC 
ACAGGCTCGG GCATCGCAGA GCCTGGACCA CACGATACAC TTTTGCACTT CGCTGCGAAG 
CGCGGACTAC GCAAGGTTGC ACTCTTTCTG CTCCAGCAAC CAGGGGGAAG AGAGGCTCTC 
CGGCTTCCCA ACAAACAGGG TCGCACACCG GCCAAGGTCG CCCAGAAGAG GGGACACTCT 
CAGCTCCACA AACTGCTCAC GGAGGTTGAC AATCTGCCCG AGCTGGAGAC GAAAACATCT 
AAGTGGTGTT ACCCAGAAGG CAGAGTCCTG CAACACCACC CCAAGCTAAA CACTTACACC 
CTTACCCTGG AGGATGTGCC CAGGAGCTCC CCACCAAGCC TTCAGTGTGA TGTGGAGGAG 
CTCCAGCGTC TCATTCAGTC ACATCAGAAG GAAGATCCTC CAACACCGCA TAAGTCAGCA 
CTGCTGATTC TCAATAGAGA CTCCAGTAAT AGCACAGAGA CAGATGACTG CCTTCCGCCC 
AACTCAGTGG AACCTGAGCA TCAGGAGCAC AACAAACAAG AAGAAACTTG CAAATCGCTT 
TTAAACAGCC TTACCTTTGA GGATGGATTG TGTGTTCATC AAAACGGCAC AGGAGATCAT 
GTCCAGTTGC TGGATGGGGA CGTGGAGCCT GACCTGCCAG CTGTAGGAGA TACAGGGAAT 
GCATTAGATA AGACTGACTG TTTGCAGCGG CGGGTAGATG GTGATTCTGT GGCTTTCAGT 
GCCCCTTCCT GTGGAAACCA GCGGGAGGAA GCCGATATAG AGCTCTGCAT AATCAGTGCC 
AAGCAGGGGG TTGCTTCACA GCCTGATAAA GGGAAGCAAG AAGAGCAAGA GGCTTGCCAG 
AAGAGGTCAG AGACTGAAAA GGACTATAAC ACTGAATCTG GCAACCTTGG GCTTCAAGAC 
ATGGGCCACG CACAATCTCA GGGTCTCCTC CCCACGGAAA CTGGTCAGCC TACACAAAGG 
GAGGTGGAGA GTGAGGGGAG TACAGATGTC TGTTGGCGTG GGGACTTACC AGAGGAAAGG 
GAAGGGGACA TGAGTAGCAA CCAGGAAATA GACTCATATT CTTCAGCACT TTCTTCCCAT 
AATCTAGAGG GAAACAGCCA GCACGATGGT GAGACTGAGC CCCAGTTGCC TACACAATGC 
TCAGAGGGAA CAGATGAAGC TAAATTGACT TTAATTCAGG TAAATTCTGA AGTTACTTTG 
TCTATACGAA GCGAGGATAC CGTTATTTCT GACAGTGTTG ACAAGCCACA TGGTCTCTTA 
GAGACGGAGA CAGGAAATCT CAGCGAACAC ACCAAAGGCA ACACTGAGTC AGCTGCTGAT 
TTGGTTGCGG ATGACGCTAG TGTAAAGCAG AACTCTGGAT TTAGCGTTTT AGAGGGGGAT 
CTTTTGGAAT CATTAAGTCT ACGGCCAAAC ATGGCACCGG GACCAGTGCA TAAGGTTCAG 
CAAGGTCTGT CACCAGTTCC TGAGACTTCC TCTCATGTAG GTCCAGCTTT GGACCCCTTT 
TCTGAGACTA CAGAAAGTTT TCAACAGCCG AATCAATCCA CACAGCTTTT AGAAACTGCA 
TTAGAATGTG AGGATGTTTC ATTAAATAAC TCAACCGGTG ACATTAATGC AATAGCAGGG 
GTTTCTGTTG ACATTTCTGC AGAGGTTTCA CTTTGCCAGA ATGAAGGGTG TCCTCCTGAT 
GGAAGCAAAG AAGAGGTAGC CCCCAGTGAG AGGATACCAG ACCTTAAAGA GCTTACGGAA 
GAGCCGGAAT CAGTTGAGGA CATTATTGTC TCAGATGTCT CAGATTTGGA TGACAAATCT 
CAACCTTTTG AAGAGTCTTC TTTGCCTATC GACTCTGAAT GTTTTATAAG AGGTGATTTC 
CAAAACGATG AGTCTACAGT ACAGTCTTCT GAATGTACTG GAGGTGACAT AAATGTAGGA 
ACACTCTTAG GAGAAGAAGA GGCAACTGAA AAAATAGAAC AGCCAGACTC TTCACTTAAA 
CTGGTTGAGC TAATTCCTGA GGAATATACT GGAGATGCCA CAAACGAAAA TAAAAAAATT 
GAGCCAGAGG AACAATCTAT TTTACTGCCA ACTTTGCAAG GGGAATCAGG ATTTAAGGAT 
GTTGCCACAG AGGAACCTGT ACAGGAAAGG GAAATTGACT CCACTTCAGA GGAAACAATC 
AGCGATCTCC CTGATCCATC GGAAATCACA AACAATGATT GTGCTGAATC CAATGCACAC 
TGCCAGCTCT CAGAATCCTC CAAGGAGAGC GCAAACACAT CCTCAGAACC TGCTGAATGT 
GTGAATGACC TAGACTTGTC TTGTGCTTTA CCTCAAACAG AAATCGAAGG CAGCAGACCT 
TCTGAGGGCC CCTTAATTGA TGGAGTAACA ATATGTGCTG AAATTCCCAT TGATGACAAA 
GGTGTTGACA TGGCTACCAA TATGAGTAAG ACGACCATCT CTGAGGATTG TCTGTCCTCT 
CCTATGGACA CCACCAGCTG TTCAGTTCTG GAGAGCTCCT CCATGTCTGA CCCACTCAAT 
GCCTCATCCT CAACAGCTGA GCTCACTTCC CCTTTGGATA TTAATAGTGG ATTGCATCAG 
GATTTGGAAC ACACTTCCCC AGTGGATATA GACAATGGTT TGCCTGAGGA ACCGCCGCCG 
TCAGACACAC CTGATTGCCT GAATGGATTA CATCAAGATT CTCCTGATAG TTTGATGGAG 
CACAGTGGAA CTCCTCAGCC ATGTTCTGAG GAACAGTGTG TGGATCAAAT CACAGATGAC 
AGCACTTCAG CAGAGGATGC AGCGGACGGA CACCAAGACT CCTCAGTAGA CATAGCAAAT 
TCCGAAGCGA TCCACAGAGA GACCGAAACA TTTTATTCAG TTGATGCCAG TCAAGACTCT 
GTACTCCAGG AGAAGAAATC TTCTTCAGTG AGCTCAGAAA TCCCCTCTTT GACCCCCTCA 
CCTGAGAGCA TACGTCACAA AGAGTCGCCA AGCACTATGC GAGGGTCCGC CAGCGATGGA 
GACTCTCTGT TTAGTCAGGA TCTGGGCGAG GACAGCGTTT TTAATAATAA GGCTGAGGAC 
AGCATTACCA TCACCAGTGG CGTGTCCATG TCCTATTCGT CCTCCACAGA TGACTCTTCC 
AGCTTGGGAA CCACTTGCGC CACCCCTCAG ACTGGCACAG AGGCTCCTGC ATCAGACCCC 
TTTCAGGAAC AGCCCACATC TACTGGAGCT TCTGGGGAAA CAGAGGAGGA AGAGAAAAAG 
GACCGTCTCA CTGAGGTTCC GGAGCGATCG GCCATTTTAC GGACCACCAT ACGATCACTG 
TCCCCTTTTC GAAGACACAG TTGGGGGCCG GGCAAAAATT TGGCAGGGGA GACAGAGATG 
AGTCAGCGCA GTGCGGCACA AGGTGAAGTG GAGCGCAGAT CTGTTGGTCA TAGGAGAAGC 
ATGAGCTGGT GTCCTTCTGT TGTCCTGCGC TCTGAAAATG ATGAAATGAA TGAAAGAAGT 
TACAGTCTGG AAGGACTTGC ACCAGAGAAG GAGGGAAGGA AAAGTCTGGT TCAGCAGCGT 
GTCGGAGAGT CCAGCCAGGA GCCCAAATGT CCATCAAAGG TGGAAAAAGA AGACAGAGGC 
TCGCTTGTGT CTCTGACTGA AGAAGAGCAG GAGTCAGATC TAGGCGACTG CAGCAGTTTG 
GGCAGTCAGA AGTCCATCCA GTCGGGTCGG AGATGTGTCC CACATTCACA ACCCATCCTC 
ACCAAATCTG TGTCCATGGT GGCCATCAGT CAGAAGGACC TGGACGCAAT GGGACGGCTG 
CGACCCAAGC GACGAATCTC CTTCTCCTTC AGCATCTCGC CGATGCTCCC CAAATCTAAA 
ACTCTCTTTT CTCTTGGCTC TTCATCCAGT GATGAGGAGG AGGCAGTCAG ATTGAGTTCA 
TTCACTGGAA CATCAGGATC ATTAGAGTAC AGTATATCAG AGGAGGATCC TGGGCCATTA 
CGGAGTGACA GTGAAGGCAA AATTGGAGGA ACCAAAGTGA GCAGGACATT CAGTTACCTC 
AAGAGCAAGA TGAGCAAGAA GAACAAGCAG GAGAAGGAGA AAGAGAAGAA TAAGGAGCGT 
GAGCGAGATG CCAAAGACAA GGAGAGAAGA GCCTCAAACG GACATCTCTT CACTACGGTC 
ATGACTGTAC CCACAATCCC CTGCCACCAG TGCAACAAAC TCATCAACAC TAAAGACTCT 
ATCCTCTGCA CCAATTGCAA TGCACAAGTC CACAAAGGCT GCAAAGAGAG TCTTCCTGTC 
TGCGCTAAAG TCAAGATGAA GCAGCAAAAA CAGCAGCAGA CTGTTACAGA TTCTGCCTCG 
ACGCCTAGTG TCACCCTCAG AAGTAAAACT CTTCCAGCAA GGGATCGTCC GTGGTCCACA 
ATATCCATTC CCGATGACCA GCCGGCTCCC CTCGCTCCTC CTCGGAAAAG CCCCAGCAGC 
ATCATGTCTT TCAACAGCAA CCCACTGTCC AAGAGCATGT CCATTAATAA CATAGCAGGA 
CAAATGGACG ACACTCCTCT TAAGAAGTTT CTGTCCCAGT CAACGGACTC TCTTCACAAG 
ACCAGTAAAG TCACGGAGTC TACAGAATCT CTTACTGATG AGGGTACAGA GATGATGGAC 
AGCCAGCTGA TGGGAGAATT TGAAGCTGAC GCCAAAGAGC TGGAGGCTGA TTCTTATAGC 
TTCACGGTAG ACAAGAAATT TCTTAAGCAG CTTAAAAAGG ATGTTATCAA AAGGCAAGAC 
GTCATCTATG AGCTCATCCA GACAGAAATG CACCATGTAA GGACCTTAAA GATCATGGCT 
GATGTTTACA GCAAAGGACT ACTGAGAGAA GTTCAACTAG AAGCTCAGAC GGTGGAGAAG 
ATGTTCCCCA TGTTGGACGA TGTTCTGGAT TTGCACACAC AGTTCTTTTC ACACCTGCTT 
GAAAGAAAGA AGGAATCAGG GAGCCAAGAA GAGGGCGGCT TTGTCATCCA AAGGATTGGC 
GATGTGCTGG TTTCTCAGTT CTCAGGTTCC AGTGCTGAGA GAATGAAGAA GGTCTATGGG 
AAGTTCTGCG GCAGGCACAA TGAAGCTGTG AACTTCTACA AGGAGCTTCA CACCAAAGAC 
AAACGCTTTC AAGCTTTCAT CAAGAAAAAG ATGAGCAGCC CTGTTGTCCG ACGGCTAAGC 
ATTCCTGAGT GTATATTGTT AGTCACACAA CGAATCACAA AATATCCCGT GTTACTACAG 
AGAATACTGC AGCACACGAA AGAAAACGAG GAGGACCATG CAGACGTTAG TCAAGGTCTG 
AAGCTGGTGA AGGAGGTGAT TGCTGCCGTG GACAATAAAG TGAATGAGCA TGAGAAGAAG 
AAGAGGCTAA AGGAAGTGTA CAGCCGGACG GACAACAAGT CCATCATGAG GATGAAGAGC 
GGGCAGATGT TTGCCAGAGA GGATCTGTTA CGTGGGCGGA AGTTAATTCA CGACGGGCCG 
CTGCAGCTCA AGAACTCAGC TGGCAGACTC AAGGATGTCC AGGCTCTGCT GCTGAGGGAC 
GTGATTGTGT TCCTGCAGGA GAAAGATCAG AAGTACATTT TCGCCTCTTT GGACCAGCGC 
TCCACCGTCA TCTCCCTGCA GAAGCTCATT GTGAGGGAAG TAGCCAATGA GGAGAAAGCT 
CTGTTTCTGA TCACAGCAGG CATCGAGAAG CCCGAGATGG TAGAAGTGTA CACAAGCTCC 
AAAGAGGAGA GAAACACATG GATGCAGCTC ATTCAGGAGG CCATGCAGTC TATACTGTCC 
ATTTCCTCCA GGGAGAGAGA TGATGATGAA GGTGTGCCAA GTGAAAACGA AGAGGACAGG 
AGACTTCAGG AAATCAAGGT CAAGGAGTTC AGAGATCAGC TGCACAAAAG AGACGAGCAG 
ATCTTGACGT TACTGGAGGA GAAGGTGAAG TTGTTCAGGG ACATGTGTGA CTGTGGTGCA 
CCGGACGAGA CGGGGCTTCG TAATCGAATG CTCTTCAGGG CCACGACGGA TGATGTCACC 
AGAGGAGAGC CAATCATGAA GGAGGCTCTG AAGGAAGTTG AAACGCTGCA GGTTCTGGTT 
AATGACAGTC TTGGCGGTGC GGTTCAGGAA GGTGTGTGCG CGGCTGGACA TGTGACTTTG 
CCACGACGAG CGGAAACATT TGGAGGTTTC GACAGTCACC AGATGAACAT CTCTAAGAGC 
AAAGATGGAG ACAGAGAGGA GCTGGAGGAG TCTGCAGAGC TCCGCAGGAC TGAGTCAGAC 
AGTGTGTTGA AGAAGACGAG TAAGACCAAC CCTTTACTGC TGGTCAGGAG AAACAATGAG 
CAGGTTCTTC AGAGCGTTGC TCATCTTCAT GACCTGCTGA ACACATTACA AGCTGTGGTG 
GTCCAGCAGG ACACCTTCAT CGAAGACCAG CGTCACGCTC ATCCCTCCTC ACGCCACCCA 
TCCATCTCCT CCTCTACCTC CTCTTTATCA TCCACCTCAT CCCGGCCGAG CTCTCTAATC 
GAGCAGGAGA AACAGCGCAG CCTGGAGAAG CAGCGGCAGG AGGTGGCCAG CCTCCAAAAA 
CAGCAGGCGG CCCACGCCGA GGAGAGGCGG CGCAGGGAAA AGCAGTGGGA GACCCGAGAG 
AAAGAGCTAA CCCAGAGGGA GATGCAGATC CAGAGCCAAG AAGAGGAGGC GCAGAGGAGA 
GCCAGACAGC TGGAGGAGGA GAAACAGGAG CTGCAGAGTA AGAAAGAGGA GTATCAGAGA 
GACCTGGCAC GACTGCGGGA CAGCCAGAAG AGACTGGAGA GAGAGAGAGA GCAGGTGCAG 
AGAGAGGCAG ATGAGCTTAA ACTGACTGAG GAAGTGACAA AGAACCGGTC TCCGTCAACC 
ACGTCTGAAG ACTCCACGAA GTTCCAGAGC ACCGGTTCAT TAGACCGTGA CCCATCAGAG 
ATGGAGCTCT CCTCCTCCCC GTCAGCCAGA GACTTCCTGT CCCGCATGGA CTCCAAACGC 
AAAGGGAAAA ACCTCAATCC ATTCTCATCC AACTCAAGCC AGAAGAGCCA GAACAGCGAG 
GCTCAGAGCC AGATGCCCAG CCGTCTGCTC CAGCTGGCCA AACCCAAGGA GAAGAAGGAC 
AAGAAGAAGA AGAAGGGGAA AGGCCAGCAG AGCCAGACTG CAGACTCTCA GAGCACTGTT 
GTTCCAGGCG CTACCCCAGA CGGAGAGATC TTTTTCTGCT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_009296114.1
CDS	398..8059
Translation	MKLNPQQAPLYGECLLTVQLCEDECVEDEDDVEFYLLFAGSTQRHVSSTLKISHVTLQAVCPAHDRSETVRVTLCQARPGGSVDPVSEECFQFVQDLALDMAQFLVNAAGKTEGALLLDECQIPLKECERLDDTLALALRHLPLPAGWSVLGTDLDTGTCTGSGIAEPGPHDTLLHFAAKRGLRKVALFLLQQPGGREALRLPNKQGRTPAKVAQKRGHSQLHKLLTEVDNLPELETKTSKWCYPEGRVLQHHPKLNTYTLTLEDVPRSSPPSLQCDVEELQRLIQSHQKEDPPTPHKSALLILNRDSSNSTETDDCLPPNSVEPEHQEHNKQEETCKSLLNSLTFEDGLCVHQNGTGDHVQLLDGDVEPDLPAVGDTGNALDKTDCLQRRVDGDSVAFSAPSCGNQREEADIELCIISAKQGVASQPDKGKQEEQEACQKRSETEKDYNTESGNLGLQDMGHAQSQGLLPTETGQPTQREVESEGSTDVCWRGDLPEEREGDMSSNQEIDSYSSALSSHNLEGNSQHDGETEPQLPTQCSEGTDEAKLTLIQVNSEVTLSIRSEDTVISDSVDKPHGLLETETGNLSEHTKGNTESAADLVADDASVKQNSGFSVLEGDLLESLSLRPNMAPGPVHKVQQGLSPVPETSSHVGPALDPFSETTESFQQPNQSTQLLETALECEDVSLNNSTGDINAIAGVSVDISAEVSLCQNEGCPPDGSKEEVAPSERIPDLKELTEEPESVEDIIVSDVSDLDDKSQPFEESSLPIDSECFIRGDFQNDESTVQSSECTGGDINVGTLLGEEEATEKIEQPDSSLKLVELIPEEYTGDATNENKKIEPEEQSILLPTLQGESGFKDVATEEPVQEREIDSTSEETISDLPDPSEITNNDCAESNAHCQLSESSKESANTSSEPAECVNDLDLSCALPQTEIEGSRPSEGPLIDGVTICAEIPIDDKGVDMATNMSKTTISEDCLSSPMDTTSCSVLESSSMSDPLNASSSTAELTSPLDINSGLHQDLEHTSPVDIDNGLPEEPPPSDTPDCLNGLHQDSPDSLMEHSGTPQPCSEEQCVDQITDDSTSAEDAADGHQDSSVDIANSEAIHRETETFYSVDASQDSVLQEKKSSSVSSEIPSLTPSPESIRHKESPSTMRGSASDGDSLFSQDLGEDSVFNNKAEDSITITSGVSMSYSSSTDDSSSLGTTCATPQTGTEAPASDPFQEQPTSTGASGETEEEEKKDRLTEVPERSAILRTTIRSLSPFRRHSWGPGKNLAGETEMSQRSAAQGEVERRSVGHRRSMSWCPSVVLRSENDEMNERSYSLEGLAPEKEGRKSLVQQRVGESSQEPKCPSKVEKEDRGSLVSLTEEEQESDLGDCSSLGSQKSIQSGRRCVPHSQPILTKSVSMVAISQKDLDAMGRLRPKRRISFSFSISPMLPKSKTLFSLGSSSSDEEEAVRLSSFTGTSGSLEYSISEEDPGPLRSDSEGKIGGTKVSRTFSYLKSKMSKKNKQEKEKEKNKERERDAKDKERRASNGHLFTTVMTVPTIPCHQCNKLINTKDSILCTNCNAQVHKGCKESLPVCAKVKMKQQKQQQTVTDSASTPSVTLRSKTLPARDRPWSTISIPDDQPAPLAPPRKSPSSIMSFNSNPLSKSMSINNIAGQMDDTPLKKFLSQSTDSLHKTSKVTESTESLTDEGTEMMDSQLMGEFEADAKELEADSYSFTVDKKFLKQLKKDVIKRQDVIYELIQTEMHHVRTLKIMADVYSKGLLREVQLEAQTVEKMFPMLDDVLDLHTQFFSHLLERKKESGSQEEGGFVIQRIGDVLVSQFSGSSAERMKKVYGKFCGRHNEAVNFYKELHTKDKRFQAFIKKKMSSPVVRRLSIPECILLVTQRITKYPVLLQRILQHTKENEEDHADVSQGLKLVKEVIAAVDNKVNEHEKKKRLKEVYSRTDNKSIMRMKSGQMFAREDLLRGRKLIHDGPLQLKNSAGRLKDVQALLLRDVIVFLQEKDQKYIFASLDQRSTVISLQKLIVREVANEEKALFLITAGIEKPEMVEVYTSSKEERNTWMQLIQEAMQSILSISSRERDDDEGVPSENEEDRRLQEIKVKEFRDQLHKRDEQILTLLEEKVKLFRDMCDCGAPDETGLRNRMLFRATTDDVTRGEPIMKEALKEVETLQVLVNDSLGGAVQEGVCAAGHVTLPRRAETFGGFDSHQMNISKSKDGDREELEESAELRRTESDSVLKKTSKTNPLLLVRRNNEQVLQSVAHLHDLLNTLQAVVVQQDTFIEDQRHAHPSSRHPSISSSTSSLSSTSSRPSSLIEQEKQRSLEKQRQEVASLQKQQAAHAEERRRREKQWETREKELTQREMQIQSQEEEAQRRARQLEEEKQELQSKKEEYQRDLARLRDSQKRLEREREQVQREADELKLTEEVTKNRSPSTTSEDSTKFQSTGSLDRDPSEMELSSSPSARDFLSRMDSKRKGKNLNPFSSNSSQKSQNSEAQSQMPSRLLQLAKPKEKKDKKKKKGKGQQSQTADSQSTVVPGATPDGEIFFC

Target ORF information:

RefSeq Version	XM_009297839.3
Organism	Danio rerio(zebrafish)
Definition	Danio rerio wu:fi15d04 (wu:fi15d04), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_009297839.3

ORF Insert Sequence:

ATGAAACTGA ATCCACAGCA AGCCCCATTA TATGGAGAGT GTCTGCTGAC GGTCCAGCTC 
TGTGAGGATG AGTGTGTTGA GGATGAGGAT GATGTGGAGT TTTACCTGCT CTTTGCTGGA 
TCCACACAGA GACATGTGTC CAGCACACTG AAGATCAGCC ATGTCACGCT GCAGGCCGTC 
TGTCCAGCTC ATGATCGCAG TGAAACGGTA CGGGTCACAC TATGCCAGGC TCGTCCCGGC 
GGCTCAGTGG ACCCAGTGTC CGAGGAGTGT TTCCAGTTTG TCCAGGACCT GGCTCTGGAC 
ATGGCCCAGT TCCTGGTAAA TGCGGCGGGG AAAACAGAAG GAGCGCTGCT GCTGGACGAG 
TGTCAGATCC CACTGAAAGA GTGCGAGCGG CTGGACGATA CTCTTGCTCT GGCTCTCCGG 
CATCTGCCTT TGCCTGCTGG ATGGAGCGTT TTGGGCACAG ATTTGGACAC GGGCACTTGC 
ACAGGCTCGG GCATCGCAGA GCCTGGACCA CACGATACAC TTTTGCACTT CGCTGCGAAG 
CGCGGACTAC GCAAGGTTGC ACTCTTTCTG CTCCAGCAAC CAGGGGGAAG AGAGGCTCTC 
CGGCTTCCCA ACAAACAGGG TCGCACACCG GCCAAGGTCG CCCAGAAGAG GGGACACTCT 
CAGCTCCACA AACTGCTCAC GGAGGTTGAC AATCTGCCCG AGCTGGAGAC GAAAACATCT 
AAGTGGTGTT ACCCAGAAGG CAGAGTCCTG CAACACCACC CCAAGCTAAA CACTTACACC 
CTTACCCTGG AGGATGTGCC CAGGAGCTCC CCACCAAGCC TTCAGTGTGA TGTGGAGGAG 
CTCCAGCGTC TCATTCAGTC ACATCAGAAG GAAGATCCTC CAACACCGCA TAAGTCAGCA 
CTGCTGATTC TCAATAGAGA CTCCAGTAAT AGCACAGAGA CAGATGACTG CCTTCCGCCC 
AACTCAGTGG AACCTGAGCA TCAGGAGCAC AACAAACAAG AAGAAACTTG CAAATCGCTT 
TTAAACAGCC TTACCTTTGA GGATGGATTG TGTGTTCATC AAAACGGCAC AGGAGATCAT 
GTCCAGTTGC TGGATGGGGA CGTGGAGCCT GACCTGCCAG CTGTAGGAGA TACAGGGAAT 
GCATTAGATA AGACTGACTG TTTGCAGCGG CGGGTAGATG GTGATTCTGT GGCTTTCAGT 
GCCCCTTCCT GTGGAAACCA GCGGGAGGAA GCCGATATAG AGCTCTGCAT AATCAGTGCC 
AAGCAGGGGG TTGCTTCACA GCCTGATAAA GGGAAGCAAG AAGAGCAAGA GGCTTGCCAG 
AAGAGGTCAG AGACTGAAAA GGACTATAAC ACTGAATCTG GCAACCTTGG GCTTCAAGAC 
ATGGGCCACG CACAATCTCA GGGTCTCCTC CCCACGGAAA CTGGTCAGCC TACACAAAGG 
GAGGTGGAGA GTGAGGGGAG TACAGATGTC TGTTGGCGTG GGGACTTACC AGAGGAAAGG 
GAAGGGGACA TGAGTAGCAA CCAGGAAATA GACTCATATT CTTCAGCACT TTCTTCCCAT 
AATCTAGAGG GAAACAGCCA GCACGATGGT GAGACTGAGC CCCAGTTGCC TACACAATGC 
TCAGAGGGAA CAGATGAAGC TAAATTGACT TTAATTCAGG TAAATTCTGA AGTTACTTTG 
TCTATACGAA GCGAGGATAC CGTTATTTCT GACAGTGTTG ACAAGCCACA TGGTCTCTTA 
GAGACGGAGA CAGGAAATCT CAGCGAACAC ACCAAAGGCA ACACTGAGTC AGCTGCTGAT 
TTGGTTGCGG ATGACGCTAG TGTAAAGCAG AACTCTGGAT TTAGCGTTTT AGAGGGGGAT 
CTTTTGGAAT CATTAAGTCT ACGGCCAAAC ATGGCACCGG GACCAGTGCA TAAGGTTCAG 
CAAGGTCTGT CACCAGTTCC TGAGACTTCC TCTCATGTAG GTCCAGCTTT GGACCCCTTT 
TCTGAGACTA CAGAAAGTTT TCAACAGCCG AATCAATCCA CACAGCTTTT AGAAACTGCA 
TTAGAATGTG AGGATGTTTC ATTAAATAAC TCAACCGGTG ACATTAATGC AATAGCAGGG 
GTTTCTGTTG ACATTTCTGC AGAGGTTTCA CTTTGCCAGA ATGAAGGGTG TCCTCCTGAT 
GGAAGCAAAG AAGAGGTAGC CCCCAGTGAG AGGATACCAG ACCTTAAAGA GCTTACGGAA 
GAGCCGGAAT CAGTTGAGGA CATTATTGTC TCAGATGTCT CAGATTTGGA TGACAAATCT 
CAACCTTTTG AAGAGTCTTC TTTGCCTATC GACTCTGAAT GTTTTATAAG AGGTGATTTC 
CAAAACGATG AGTCTACAGT ACAGTCTTCT GAATGTACTG GAGGTGACAT AAATGTAGGA 
ACACTCTTAG GAGAAGAAGA GGCAACTGAA AAAATAGAAC AGCCAGACTC TTCACTTAAA 
CTGGTTGAGC TAATTCCTGA GGAATATACT GGAGATGCCA CAAACGAAAA TAAAAAAATT 
GAGCCAGAGG AACAATCTAT TTTACTGCCA ACTTTGCAAG GGGAATCAGG ATTTAAGGAT 
GTTGCCACAG AGGAACCTGT ACAGGAAAGG GAAATTGACT CCACTTCAGA GGAAACAATC 
AGCGATCTCC CTGATCCATC GGAAATCACA AACAATGATT GTGCTGAATC CAATGCACAC 
TGCCAGCTCT CAGAATCCTC CAAGGAGAGC GCAAACACAT CCTCAGAACC TGCTGAATGT 
GTGAATGACC TAGACTTGTC TTGTGCTTTA CCTCAAACAG AAATCGAAGG CAGCAGACCT 
TCTGAGGGCC CCTTAATTGA TGGAGTAACA ATATGTGCTG AAATTCCCAT TGATGACAAA 
GGTGTTGACA TGGCTACCAA TATGAGTAAG ACGACCATCT CTGAGGATTG TCTGTCCTCT 
CCTATGGACA CCACCAGCTG TTCAGTTCTG GAGAGCTCCT CCATGTCTGA CCCACTCAAT 
GCCTCATCCT CAACAGCTGA GCTCACTTCC CCTTTGGATA TTAATAGTGG ATTGCATCAG 
GATTTGGAAC ACACTTCCCC AGTGGATATA GACAATGGTT TGCCTGAGGA ACCGCCGCCG 
TCAGACACAC CTGATTGCCT GAATGGATTA CATCAAGATT CTCCTGATAG TTTGATGGAG 
CACAGTGGAA CTCCTCAGCC ATGTTCTGAG GAACAGTGTG TGGATCAAAT CACAGATGAC 
AGCACTTCAG CAGAGGATGC AGCGGACGGA CACCAAGACT CCTCAGTAGA CATAGCAAAT 
TCCGAAGCGA TCCACAGAGA GACCGAAACA TTTTATTCAG TTGATGCCAG TCAAGACTCT 
GTACTCCAGG AGAAGAAATC TTCTTCAGTG AGCTCAGAAA TCCCCTCTTT GACCCCCTCA 
CCTGAGAGCA TACGTCACAA AGAGTCGCCA AGCACTATGC GAGGGTCCGC CAGCGATGGA 
GACTCTCTGT TTAGTCAGGA TCTGGGCGAG GACAGCGTTT TTAATAATAA GGCTGAGGAC 
AGCATTACCA TCACCAGTGG CGTGTCCATG TCCTATTCGT CCTCCACAGA TGACTCTTCC 
AGCTTGGGAA CCACTTGCGC CACCCCTCAG ACTGGCACAG AGGCTCCTGC ATCAGACCCC 
TTTCAGGAAC AGCCCACATC TACTGGAGCT TCTGGGGAAA CAGAGGAGGA AGAGAAAAAG 
GACCGTCTCA CTGAGGTTCC GGAGCGATCG GCCATTTTAC GGACCACCAT ACGATCACTG 
TCCCCTTTTC GAAGACACAG TTGGGGGCCG GGCAAAAATT TGGCAGGGGA GACAGAGATG 
AGTCAGCGCA GTGCGGCACA AGGTGAAGTG GAGCGCAGAT CTGTTGGTCA TAGGAGAAGC 
ATGAGCTGGT GTCCTTCTGT TGTCCTGCGC TCTGAAAATG ATGAAATGAA TGAAAGAAGT 
TACAGTCTGG AAGGACTTGC ACCAGAGAAG GAGGGAAGGA AAAGTCTGGT TCAGCAGCGT 
GTCGGAGAGT CCAGCCAGGA GCCCAAATGT CCATCAAAGG TGGAAAAAGA AGACAGAGGC 
TCGCTTGTGT CTCTGACTGA AGAAGAGCAG GAGTCAGATC TAGGCGACTG CAGCAGTTTG 
GGCAGTCAGA AGTCCATCCA GTCGGGTCGG AGATGTGTCC CACATTCACA ACCCATCCTC 
ACCAAATCTG TGTCCATGGT GGCCATCAGT CAGAAGGACC TGGACGCAAT GGGACGGCTG 
CGACCCAAGC GACGAATCTC CTTCTCCTTC AGCATCTCGC CGATGCTCCC CAAATCTAAA 
ACTCTCTTTT CTCTTGGCTC TTCATCCAGT GATGAGGAGG AGGCAGTCAG ATTGAGTTCA 
TTCACTGGAA CATCAGGATC ATTAGAGTAC AGTATATCAG AGGAGGATCC TGGGCCATTA 
CGGAGTGACA GTGAAGGCAA AATTGGAGGA ACCAAAGTGA GCAGGACATT CAGTTACCTC 
AAGAGCAAGA TGAGCAAGAA GAACAAGCAG GAGAAGGAGA AAGAGAAGAA TAAGGAGCGT 
GAGCGAGATG CCAAAGACAA GGAGAGAAGA GCCTCAAACG GACATCTCTT CACTACGGTC 
ATGACTGTAC CCACAATCCC CTGCCACCAG TGCAACAAAC TCATCAACAC TAAAGACTCT 
ATCCTCTGCA CCAATTGCAA TGCACAAGTC CACAAAGGCT GCAAAGAGAG TCTTCCTGTC 
TGCGCTAAAG TCAAGATGAA GCAGCAAAAA CAGCAGCAGA CTGTTACAGA TTCTGCCTCG 
ACGCCTAGTG TCACCCTCAG AAGTAAAACT CTTCCAGCAA GGGATCGTCC GTGGTCCACA 
ATATCCATTC CCGATGACCA GCCGGCTCCC CTCGCTCCTC CTCGGAAAAG CCCCAGCAGC 
ATCATGTCTT TCAACAGCAA CCCACTGTCC AAGAGCATGT CCATTAATAA CATAGCAGGA 
CAAATGGACG ACACTCCTCT TAAGAAGTTT CTGTCCCAGT CAACGGACTC TCTTCACAAG 
ACCAGTAAAG TCACGGAGTC TACAGAATCT CTTACTGATG AGGGTACAGA GATGATGGAC 
AGCCAGCTGA TGGGAGAATT TGAAGCTGAC GCCAAAGAGC TGGAGGCTGA TTCTTATAGC 
TTCACGGTAG ACAAGAAATT TCTTAAGCAG CTTAAAAAGG ATGTTATCAA AAGGCAAGAC 
GTCATCTATG AGCTCATCCA GACAGAAATG CACCATGTAA GGACCTTAAA GATCATGGCT 
GATGTTTACA GCAAAGGACT ACTGAGAGAA GTTCAACTAG AAGCTCAGAC GGTGGAGAAG 
ATGTTCCCCA TGTTGGACGA TGTTCTGGAT TTGCACACAC AGTTCTTTTC ACACCTGCTT 
GAAAGAAAGA AGGAATCAGG GAGCCAAGAA GAGGGCGGCT TTGTCATCCA AAGGATTGGC 
GATGTGCTGG TTTCTCAGTT CTCAGGTTCC AGTGCTGAGA GAATGAAGAA GGTCTATGGG 
AAGTTCTGCG GCAGGCACAA TGAAGCTGTG AACTTCTACA AGGAGCTTCA CACCAAAGAC 
AAACGCTTTC AAGCTTTCAT CAAGAAAAAG ATGAGCAGCC CTGTTGTCCG ACGGCTAAGC 
ATTCCTGAGT GTATATTGTT AGTCACACAA CGAATCACAA AATATCCCGT GTTACTACAG 
AGAATACTGC AGCACACGAA AGAAAACGAG GAGGACCATG CAGACGTTAG TCAAGGTCTG 
AAGCTGGTGA AGGAGGTGAT TGCTGCCGTG GACAATAAAG TGAATGAGCA TGAGAAGAAG 
AAGAGGCTAA AGGAAGTGTA CAGCCGGACG GACAACAAGT CCATCATGAG GATGAAGAGC 
GGGCAGATGT TTGCCAGAGA GGATCTGTTA CGTGGGCGGA AGTTAATTCA CGACGGGCCG 
CTGCAGCTCA AGAACTCAGC TGGCAGACTC AAGGATGTCC AGGCTCTGCT GCTGAGGGAC 
GTGATTGTGT TCCTGCAGGA GAAAGATCAG AAGTACATTT TCGCCTCTTT GGACCAGCGC 
TCCACCGTCA TCTCCCTGCA GAAGCTCATT GTGAGGGAAG TAGCCAATGA GGAGAAAGCT 
CTGTTTCTGA TCACAGCAGG CATCGAGAAG CCCGAGATGG TAGAAGTGTA CACAAGCTCC 
AAAGAGGAGA GAAACACATG GATGCAGCTC ATTCAGGAGG CCATGCAGTC TATACTGTCC 
ATTTCCTCCA GGGAGAGAGA TGATGATGAA GGTGTGCCAA GTGAAAACGA AGAGGACAGG 
AGACTTCAGG AAATCAAGGT CAAGGAGTTC AGAGATCAGC TGCACAAAAG AGACGAGCAG 
ATCTTGACGT TACTGGAGGA GAAGGTGAAG TTGTTCAGGG ACATGTGTGA CTGTGGTGCA 
CCGGACGAGA CGGGGCTTCG TAATCGAATG CTCTTCAGGG CCACGACGGA TGATGTCACC 
AGAGGAGAGC CAATCATGAA GGAGGCTCTG AAGGAAGTTG AAACGCTGCA GGTTCTGGTT 
AATGACAGTC TTGGCGGTGC GGTTCAGGAA GGTGTGTGCG CGGCTGGACA TGTGACTTTG 
CCACGACGAG CGGAAACATT TGGAGGTTTC GACAGTCACC AGATGAACAT CTCTAAGAGC 
AAAGATGGAG ACAGAGAGGA GCTGGAGGAG TCTGCAGAGC TCCGCAGGAC TGAGTCAGAC 
AGTGTGTTGA AGAAGACGAG TAAGACCAAC CCTTTACTGC TGGTCAGGAG AAACAATGAG 
CAGGTTCTTC AGAGCGTTGC TCATCTTCAT GACCTGCTGA ACACATTACA AGCTGTGGTG 
GTCCAGCAGG ACACCTTCAT CGAAGACCAG CGTCACGCTC ATCCCTCCTC ACGCCACCCA 
TCCATCTCCT CCTCTACCTC CTCTTTATCA TCCACCTCAT CCCGGCCGAG CTCTCTAATC 
GAGCAGGAGA AACAGCGCAG CCTGGAGAAG CAGCGGCAGG AGGTGGCCAG CCTCCAAAAA 
CAGCAGGCGG CCCACGCCGA GGAGAGGCGG CGCAGGGAAA AGCAGTGGGA GACCCGAGAG 
AAAGAGCTAA CCCAGAGGGA GATGCAGATC CAGAGCCAAG AAGAGGAGGC GCAGAGGAGA 
GCCAGACAGC TGGAGGAGGA GAAACAGGAG CTGCAGAGTA AGAAAGAGGA GTATCAGAGA 
GACCTGGCAC GACTGCGGGA CAGCCAGAAG AGACTGGAGA GAGAGAGAGA GCAGGTGCAG 
AGAGAGGCAG ATGAGCTTAA ACTGACTGAG GAAGTGACAA AGAACCGGTC TCCGTCAACC 
ACGTCTGAAG ACTCCACGAA GTTCCAGAGC ACCGGTTCAT TAGACCGTGA CCCATCAGAG 
ATGGAGCTCT CCTCCTCCCC GTCAGCCAGA GACTTCCTGT CCCGCATGGA CTCCAAACGC 
AAAGGGAAAA ACCTCAATCC ATTCTCATCC AACTCAAGCC AGAAGAGCCA GAACAGCGAG 
GCTCAGAGCC AGATGCCCAG CCGTCTGCTC CAGCTGGCCA AACCCAAGGA GAAGAAGGAC 
AAGAAGAAGA AGAAGGGGAA AGGCCAGCAG AGCCAGACTG CAGACTCTCA GAGCACTGTT 
GTTCCAGGCG CTACCCCAGA CGGAGAGATC TTTTTCTGCT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

wu:fi15d04 ( XM_009297841.2 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_009296116.1 Danio rerio wu:fi15d04 (wu:fi15d04), transcript variant X8, mRNA.

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CloneID	ODa39935
Clone ID Related Accession (Same CDS sequence)	XM_009297841.2
Accession Version	XM_009297841.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7584bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-23
Organism	Danio rerio(Zebrafish)
Product	A-kinase anchor protein 13 isoform X8
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007136.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_009297841.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAGGGTG TTCCACAAGT GGCCAGTGCC ATGAAACTGA ATCCACAGCA AGCCCCATTA 
TATGGAGAGT GTCTGCTGAC GGTCCAGCTC TGTGAGGATG AGTGTGTTGA GGATGAGGAT 
GATGTGGAGT TTTACCTGCT CTTTGCTGGA TCCACACAGA GACATGTGTC CAGCACACTG 
AAGATCAGCC ATGTCACGCT GCAGGCCGTC TGTCCAGCTC ATGATCGCAG TGAAACGGTA 
CGGGTCACAC TATGCCAGGC TCGTCCCGGC GGCTCAGTGG ACCCAGTGTC CGAGGAGTGT 
TTCCAGTTTG TCCAGGACCT GGCTCTGGAC ATGGCCCAGT TCCTGGTAAA TGCGGCGGGG 
AAAACAGAAG GAGCGCTGCT GCTGGACGAG TGTCAGATCC CACTGAAAGA GTGCGAGCGG 
CTGGACGATA CTCTTGCTCT GGCTCTCCGG CATCTGCCTT TGCCTGCTGG ATGGAGCGTT 
TTGGGCACAG ATTTGGACAC GGGCACTTGC ACAGGCTCGG GCATCGCAGA GCCTGGACCA 
CACGATACAC TTTTGCACTT CGCTGCGAAG CGCGGACTAC GCAAGGTTGC ACTCTTTCTG 
CTCCAGCAAC CAGGGGGAAG AGAGGCTCTC CGGCTTCCCA ACAAACAGGG TCGCACACCG 
GCCAAGGTCG CCCAGAAGAG GGGACACTCT CAGCTCCACA AACTGCTCAC GGAGGTTGAC 
AATCTGCCCG AGCTGGAGAC GAAAACATCT AAGTGGTGTT ACCCAGAAGG CAGAGTCCTG 
CAACACCACC CCAAGCTAAA CACTTACACC CTTACCCTGG AGGATGTGCC CAGGAGCTCC 
CCACCAAGCC TTCAGTGTGA TGTGGAGGAG CTCCAGCGTC TCATTCAGTC ACATCAGAAG 
GAAGATCCTC CAACACCGCA TAAGTCAGCA CTGCTGATTC TCAATAGAGA CTCCAGTAAT 
AGCACAGAGA CAGATGACTG CCTTCCGCCC AACTCAGTGG AACCTGAGCA TCAGGAGCAC 
AACAAACAAG AAGAAACTTG CAAATCGCTT TTAAACAGCC TTACCTTTGA GGATGGATTG 
TGTGTTCATC AAAACGGCAC AGGAGATCAT GTCCAGTTGC TGGATGGGGA CGTGGAGCCT 
GACCTGCCAG CTGTAGGAGA TACAGGGAAT GCATTAGATA AGACTGACTG TTTGCAGCGG 
CGGGTAGATG GTGATTCTGT GGCTTTCAGT GCCCCTTCCT GTGGAAACCA GCGGGAGGAA 
GCCGATATAG AGCTCTGCAT AATCAGTGCC AAGCAGGGGG TTGCTTCACA GCCTGATAAA 
GGGAAGCAAG AAGAGCAAGA GGCTTGCCAG AAGAGGTCAG AGACTGAAAA GGACTATAAC 
ACTGAATCTG GCAACCTTGG GCTTCAAGAC ATGGGCCACG CACAATCTCA GGGTCTCCTC 
CCCACGGAAA CTGGTCAGCC TACACAAAGG GAGGTGGAGA GTGAGGGGAG TACAGATGTC 
TGTTGGCGTG GGGACTTACC AGAGGAAAGG GAAGGGGACA TGAGTAGCAA CCAGGAAATA 
GACTCATATT CTTCAGCACT TTCTTCCCAT AATCTAGAGG GAAACAGCCA GCACGATGGT 
GAGACTGAGC CCCAGTTGCC TACACAATGC TCAGAGGGAA CAGATGAAGC TAAATTGACT 
TTAATTCAGG TAAATTCTGA AGTTACTTTG TCTATACGAA GCGAGGATAC CGTTATTTCT 
GACAGTGTTG ACAAGCCACA TGGTCTCTTA GAGACGGAGA CAGGAAATCT CAGCGAACAC 
ACCAAAGGCA ACACTGAGTC AGCTGCTGAT TTGGTTGCGG ATGACGCTAG TGTAAAGCAG 
AACTCTGGAT TTAGCGTTTT AGAGGGGGAT CTTTTGGAAT CATTAAGTCT ACGGCCAAAC 
ATGGCACCGG GACCAGTGCA TAAGGTTCAG CAAGGTCTGT CACCAGTTCC TGAGACTTCC 
TCTCATGTAG GTCCAGCTTT GGACCCCTTT TCTGAGACTA CAGAAAGTTT TCAACAGCCG 
AATCAATCCA CACAGCTTTT AGAAACTGCA TTAGAATGTG AGGATGTTTC ATTAAATAAC 
TCAACCGGTG ACATTAATGC AATAGCAGGG GTTTCTGTTG ACATTTCTGC AGAGGTTTCA 
CTTTGCCAGA ATGAAGGGTG TCCTCCTGAT GGAAGCAAAG AAGAGGTAGC CCCCAGTGAG 
AGGATACCAG ACCTTAAAGA GCTTACGGAA GAGCCGGAAT CAGTTGAGGA CATTATTGTC 
TCAGATGTCT CAGATTTGGA TGACAAATCT CAACCTTTTG AAGAGTCTTC TTTGCCTATC 
GACTCTGAAT GTTTTATAAG AGGTGATTTC CAAAACGATG AGTCTACAGT ACAGTCTTCT 
GAATGTACTG GAGGTGACAT AAATGTAGGA ACACTCTTAG GAGAAGAAGA GGCAACTGAA 
AAAATAGAAC AGCCAGACTC TTCACTTAAA CTGGTTGAGC TAATTCCTGA GGAATATACT 
GGAGATGCCA CAAACGAAAA TAAAAAAATT GAGCCAGAGG AACAATCTAT TTTACTGCCA 
ACTTTGCAAG GGGAATCAGG ATTTAAGGAT GTTGCCACAG AGGAACCTGT ACAGGAAAGG 
GAAATTGACT CCACTTCAGA GGAAACAATC AGCGATCTCC CTGATCCATC GGAAATCACA 
AACAATGATT GTGCTGAATC CAATGCACAC TGCCAGCTCT CAGAATCCTC CAAGGAGAGC 
GCAAACACAT CCTCAGAACC TGCTGAATGT GTGAATGACC TAGACTTGTC TTGTGCTTTA 
CCTCAAACAG AAATCGAAGG CAGCAGACCT TCTGAGGGCC CCTTAATTGA TGGAGTAACA 
ATATGTGCTG AAATTCCCAT TGATGACAAA GGTGTTGACA TGGCTACCAA TATGAGTAAG 
ACGACCATCT CTGAGGATTG TCTGTCCTCT CCTATGGACA CCACCAGCTG TTCAGTTCTG 
GAGAGCTCCT CCATGTCTGA CCCACTCAAT GCCTCATCCT CAACAGCTGA GCTCACTTCC 
CCTTTGGATA TTAATAGTGG ATTGCATCAG GATTTGGAAC ACACTTCCCC AGTGGATATA 
GACAATGGTT TGCCTGAGGA ACCGCCGCCG TCAGACACAC CTGATTGCCT GAATGGATTA 
CATCAAGATT CTCCTGATAG TTTGATGGAG CACAGTGGAA CTCCTCAGCC ATGTTCTGAG 
GAACAGTGTG TGGATCAAAT CACAGATGAC AGCACTTCAG CAGAGGATGC AGCGGACGGA 
CACCAAGACT CCTCAGTAGA CATAGCAAAT TCCGAAGCGA TCCACAGAGA GACCGAAACA 
TTTTATTCAG TTGATGCCAG TCAAGACTCT GTACTCCAGG AGAAGAAATC TTCTTCAGTG 
AGCTCAGAAA TCCCCTCTTT GACCCCCTCA CCTGAGAGCA TACGTCACAA AGAGTCGCCA 
AGCACTATGC GAGGGTCCGC CAGCGATGGA GACTCTCTGT TTAGTCAGGA TCTGGGCGAG 
GACAGCGTTT TTAATAATAA GGCTGAGGAC AGCATTACCA TCACCAGTGG CGTGTCCATG 
TCCTATTCGT CCTCCACAGA TGACTCTTCC AGCTTGGGAA CCACTTGCGC CACCCCTCAG 
ACTGGCACAG AGGCTCCTGC ATCAGACCCC TTTCAGGAAC AGCCCACATC TACTGGAGCT 
TCTGGGGAAA CAGAGGAGGA AGAGAAAAAG GACCGTCTCA CTGAGGTTCC GGAGCGATCG 
GCCATTTTAC GGACCACCAT ACGATCACTG TCCCCTTTTC GAAGACACAG TTGGGGGCCG 
GGCAAAAATT TGGCAGGGGA GACAGAGATG AGTCAGCGCA GTTACAGTCT GGAAGGACTT 
GCACCAGAGA AGGAGGGAAG GAAAAGTCTG GTTCAGCAGC GTGTCGGAGA GTCCAGCCAG 
GAGCCCAAAT GTCCATCAAA GGTGGAAAAA GAAGACAGAG GCTCGCTTGT GTCTCTGACT 
GAAGAAGAGC AGGAGTCAGA TCTAGGCGAC TGCAGCAGTT TGGGCAGTCA GAAGTCCATC 
CAGTCGGGTC GGAGATGTGT CCCACATTCA CAACCCATCC TCACCAAATC TGTGTCCATG 
GTGGCCATCA GTCAGAAGGA CCTGGACGCA ATGGGACGGC TGCGACCCAA GCGACGAATC 
TCCTTCTCCT TCAGCATCTC GCCGATGCTC CCCAAATCTA AAACTCTCTT TTCTCTTGGC 
TCTTCATCCA GTGATGAGGA GGAGGCAGTC AGATTGAGTT CATTCACTGG AACATCAGGA 
TCATTAGAGT ACAGTATATC AGAGGAGGAT CCTGGGCCAT TACGGAGTGA CAGTGAAGGC 
AAAATTGGAG GAACCAAAGT GAGCAGGACA TTCAGTTACC TCAAGAGCAA GATGAGCAAG 
AAGAACAAGC AGGAGAAGGA GAAAGAGAAG AATAAGGAGC GTGAGCGAGA TGCCAAAGAC 
AAGGAGAGAA GAGCCTCAAA CGGACATCTC TTCACTACGG TCATGACTGT ACCCACAATC 
CCCTGCCACC AGTGCAACAA ACTCATCAAC ACTAAAGACT CTATCCTCTG CACCAATTGC 
AATGCACAAG TCCACAAAGG CTGCAAAGAG AGTCTTCCTG TCTGCGCTAA AGTCAAGATG 
AAGCAGCAAA AACAGCAGCA GACTGTTACA GATTCTGCCT CGACGCCTAG TGTCACCCTC 
AGAAGTAAAA CTCTTCCAGC AAGGGATCGT CCGTGGTCCA CAATATCCAT TCCCGATGAC 
CAGCCGGCTC CCCTCGCTCC TCCTCGGAAA AGCCCCAGCA GCATCATGTC TTTCAACAGC 
AACCCACTGT CCAAGAGCAT GTCCATTAAT AACATAGCAG GACAAATGGA CGACACTCCT 
CTTAAGAAGT TTCTGTCCCA GTCAACGGAC TCTCTTCACA AGACCAGTAA AGTCACGGAG 
TCTACAGAAT CTCTTACTGA TGAGGGTACA GAGATGATGG ACAGCCAGCT GATGGGAGAA 
TTTGAAGCTG ACGCCAAAGA GCTGGAGGCT GATTCTTATA GCTTCACGGT AGACAAGAAA 
TTTCTTAAGC AGCTTAAAAA GGATGTTATC AAAAGGCAAG ACGTCATCTA TGAGCTCATC 
CAGACAGAAA TGCACCATGT AAGGACCTTA AAGATCATGG CTGATGTTTA CAGCAAAGGA 
CTACTGAGAG AAGTTCAACT AGAAGCTCAG ACGGTGGAGA AGATGTTCCC CATGTTGGAC 
GATGTTCTGG ATTTGCACAC ACAGTTCTTT TCACACCTGC TTGAAAGAAA GAAGGAATCA 
GGGAGCCAAG AAGAGGGCGG CTTTGTCATC CAAAGGATTG GCGATGTGCT GGTTTCTCAG 
TTCTCAGGTT CCAGTGCTGA GAGAATGAAG AAGGTCTATG GGAAGTTCTG CGGCAGGCAC 
AATGAAGCTG TGAACTTCTA CAAGGAGCTT CACACCAAAG ACAAACGCTT TCAAGCTTTC 
ATCAAGAAAA AGATGAGCAG CCCTGTTGTC CGACGGCTAA GCATTCCTGA GTGTATATTG 
TTAGTCACAC AACGAATCAC AAAATATCCC GTGTTACTAC AGAGAATACT GCAGCACACG 
AAAGAAAACG AGGAGGACCA TGCAGACGTT AGTCAAGGTC TGAAGCTGGT GAAGGAGGTG 
ATTGCTGCCG TGGACAATAA AGTGAATGAG CATGAGAAGA AGAAGAGGCT AAAGGAAGTG 
TACAGCCGGA CGGACAACAA GTCCATCATG AGGATGAAGA GCGGGCAGAT GTTTGCCAGA 
GAGGATCTGT TACGTGGGCG GAAGTTAATT CACGACGGGC CGCTGCAGCT CAAGAACTCA 
GCTGGCAGAC TCAAGGATGT CCAGGCTCTG CTGCTGAGGG ACGTGATTGT GTTCCTGCAG 
GAGAAAGATC AGAAGTACAT TTTCGCCTCT TTGGACCAGC GCTCCACCGT CATCTCCCTG 
CAGAAGCTCA TTGTGAGGGA AGTAGCCAAT GAGGAGAAAG CTCTGTTTCT GATCACAGCA 
GGCATCGAGA AGCCCGAGAT GGTAGAAGTG TACACAAGCT CCAAAGAGGA GAGAAACACA 
TGGATGCAGC TCATTCAGGA GGCCATGCAG TCTATACTGT CCATTTCCTC CAGGGAGAGA 
GATGATGATG AAGGTGTGCC AAGTGAAAAC GAAGAGGACA GGAGACTTCA GGAAATCAAG 
GTCAAGGAGT TCAGAGATCA GCTGCACAAA AGAGACGAGC AGATCTTGAC GTTACTGGAG 
GAGAAGGTGA AGTTGTTCAG GGACATGTGT GACTGTGGTG CACCGGACGA GACGGGGCTT 
CGTAATCGAA TGCTCTTCAG GGCCACGACG GATGATGTCA CCAGAGGAGA GCCAATCATG 
AAGGAGGCTC TGAAGGAAGT TGAAACGCTG CAGGTTCTGG TTAATGACAG TCTTGGCGGT 
GCGGTTCAGG AAGGTGTGTG CGCGGCTGGA CATGTGACTT TGCCACGACG AGCGGAAACA 
TTTGGAGGTT TCGACAGTCA CCAGATGAAC ATCTCTAAGA GCAAAGATGG AGACAGAGAG 
GAGCTGGAGG AGTCTGCAGA GCTCCGCAGG ACTGAGTCAG ACAGTGTGTT GAAGAAGACG 
AGTAAGACCA ACCCTTTACT GCTGGTCAGG AGAAACAATG AGCAGGTTCT TCAGAGCGTT 
GCTCATCTTC ATGACCTGCT GAACACATTA CAAGCTGTGG TGGTCCAGCA GGACACCTTC 
ATCGAAGACC AGCGTCACGC TCATCCCTCC TCACGCCACC CATCCATCTC CTCCTCTACC 
TCCTCTTTAT CATCCACCTC ATCCCGGCCG AGCTCTCTAA TCGAGCAGGA GAAACAGCGC 
AGCCTGGAGA AGCAGCGGCA GGAGGTGGCC AGCCTCCAAA AACAGCAGGC GGCCCACGCC 
GAGGAGAGGC GGCGCAGGGA AAAGCAGTGG GAGACCCGAG AGAAAGAGCT AACCCAGAGG 
GAGATGCAGA TCCAGAGCCA AGAAGAGGAG GCGCAGAGGA GAGCCAGACA GCTGGAGGAG 
GAGAAACAGG AGCTGCAGAG TAAGAAAGAG GAGTATCAGA GAGACCTGGC ACGACTGCGG 
GACAGCCAGA AGAGACTGGA GAGAGAGAGA GAGCAGGTGC AGAGAGAGGC AGATGAGCTT 
AAACTGACTG AGGAAGTGAC AAAGAACCGG TCTCCGTCAA CCACGTCTGA AGACTCCACG 
AAGTTCCAGA GCACCGGTTC ATTAGACCGT GACCCATCAG AGATGGAGCT CTCCTCCTCC 
CCGTCAGCCA GAGACTTCCT GTCCCGCATG GACTCCAAAC GCAAAGGGAA AAACCTCAAT 
CCATTCTCAT CCAACTCAAG CCAGAAGAGC CAGAACAGCG AGGCTCAGAG CCAGATGCCC 
AGCCGTCTGC TCCAGCTGGC CAAACCCAAG GAGAAGAAGG ACAAGAAGAA GAAGAAGGGG 
AAAGGCCAGC AGAGCCAGAC TGCAGACTCT CAGAGCACTG TTGTTCCAGG CGCTACCCCA 
GACGGAGAGA TCTTTTTCTG CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_009296116.1
CDS	28..7611
Translation	MKGVPQVASAMKLNPQQAPLYGECLLTVQLCEDECVEDEDDVEFYLLFAGSTQRHVSSTLKISHVTLQAVCPAHDRSETVRVTLCQARPGGSVDPVSEECFQFVQDLALDMAQFLVNAAGKTEGALLLDECQIPLKECERLDDTLALALRHLPLPAGWSVLGTDLDTGTCTGSGIAEPGPHDTLLHFAAKRGLRKVALFLLQQPGGREALRLPNKQGRTPAKVAQKRGHSQLHKLLTEVDNLPELETKTSKWCYPEGRVLQHHPKLNTYTLTLEDVPRSSPPSLQCDVEELQRLIQSHQKEDPPTPHKSALLILNRDSSNSTETDDCLPPNSVEPEHQEHNKQEETCKSLLNSLTFEDGLCVHQNGTGDHVQLLDGDVEPDLPAVGDTGNALDKTDCLQRRVDGDSVAFSAPSCGNQREEADIELCIISAKQGVASQPDKGKQEEQEACQKRSETEKDYNTESGNLGLQDMGHAQSQGLLPTETGQPTQREVESEGSTDVCWRGDLPEEREGDMSSNQEIDSYSSALSSHNLEGNSQHDGETEPQLPTQCSEGTDEAKLTLIQVNSEVTLSIRSEDTVISDSVDKPHGLLETETGNLSEHTKGNTESAADLVADDASVKQNSGFSVLEGDLLESLSLRPNMAPGPVHKVQQGLSPVPETSSHVGPALDPFSETTESFQQPNQSTQLLETALECEDVSLNNSTGDINAIAGVSVDISAEVSLCQNEGCPPDGSKEEVAPSERIPDLKELTEEPESVEDIIVSDVSDLDDKSQPFEESSLPIDSECFIRGDFQNDESTVQSSECTGGDINVGTLLGEEEATEKIEQPDSSLKLVELIPEEYTGDATNENKKIEPEEQSILLPTLQGESGFKDVATEEPVQEREIDSTSEETISDLPDPSEITNNDCAESNAHCQLSESSKESANTSSEPAECVNDLDLSCALPQTEIEGSRPSEGPLIDGVTICAEIPIDDKGVDMATNMSKTTISEDCLSSPMDTTSCSVLESSSMSDPLNASSSTAELTSPLDINSGLHQDLEHTSPVDIDNGLPEEPPPSDTPDCLNGLHQDSPDSLMEHSGTPQPCSEEQCVDQITDDSTSAEDAADGHQDSSVDIANSEAIHRETETFYSVDASQDSVLQEKKSSSVSSEIPSLTPSPESIRHKESPSTMRGSASDGDSLFSQDLGEDSVFNNKAEDSITITSGVSMSYSSSTDDSSSLGTTCATPQTGTEAPASDPFQEQPTSTGASGETEEEEKKDRLTEVPERSAILRTTIRSLSPFRRHSWGPGKNLAGETEMSQRSYSLEGLAPEKEGRKSLVQQRVGESSQEPKCPSKVEKEDRGSLVSLTEEEQESDLGDCSSLGSQKSIQSGRRCVPHSQPILTKSVSMVAISQKDLDAMGRLRPKRRISFSFSISPMLPKSKTLFSLGSSSSDEEEAVRLSSFTGTSGSLEYSISEEDPGPLRSDSEGKIGGTKVSRTFSYLKSKMSKKNKQEKEKEKNKERERDAKDKERRASNGHLFTTVMTVPTIPCHQCNKLINTKDSILCTNCNAQVHKGCKESLPVCAKVKMKQQKQQQTVTDSASTPSVTLRSKTLPARDRPWSTISIPDDQPAPLAPPRKSPSSIMSFNSNPLSKSMSINNIAGQMDDTPLKKFLSQSTDSLHKTSKVTESTESLTDEGTEMMDSQLMGEFEADAKELEADSYSFTVDKKFLKQLKKDVIKRQDVIYELIQTEMHHVRTLKIMADVYSKGLLREVQLEAQTVEKMFPMLDDVLDLHTQFFSHLLERKKESGSQEEGGFVIQRIGDVLVSQFSGSSAERMKKVYGKFCGRHNEAVNFYKELHTKDKRFQAFIKKKMSSPVVRRLSIPECILLVTQRITKYPVLLQRILQHTKENEEDHADVSQGLKLVKEVIAAVDNKVNEHEKKKRLKEVYSRTDNKSIMRMKSGQMFAREDLLRGRKLIHDGPLQLKNSAGRLKDVQALLLRDVIVFLQEKDQKYIFASLDQRSTVISLQKLIVREVANEEKALFLITAGIEKPEMVEVYTSSKEERNTWMQLIQEAMQSILSISSRERDDDEGVPSENEEDRRLQEIKVKEFRDQLHKRDEQILTLLEEKVKLFRDMCDCGAPDETGLRNRMLFRATTDDVTRGEPIMKEALKEVETLQVLVNDSLGGAVQEGVCAAGHVTLPRRAETFGGFDSHQMNISKSKDGDREELEESAELRRTESDSVLKKTSKTNPLLLVRRNNEQVLQSVAHLHDLLNTLQAVVVQQDTFIEDQRHAHPSSRHPSISSSTSSLSSTSSRPSSLIEQEKQRSLEKQRQEVASLQKQQAAHAEERRRREKQWETREKELTQREMQIQSQEEEAQRRARQLEEEKQELQSKKEEYQRDLARLRDSQKRLEREREQVQREADELKLTEEVTKNRSPSTTSEDSTKFQSTGSLDRDPSEMELSSSPSARDFLSRMDSKRKGKNLNPFSSNSSQKSQNSEAQSQMPSRLLQLAKPKEKKDKKKKKGKGQQSQTADSQSTVVPGATPDGEIFFC

Target ORF information:

RefSeq Version	XM_009297841.2
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio wu:fi15d04 (wu:fi15d04), transcript variant X8, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_009297841.2

ORF Insert Sequence:

ATGAAGGGTG TTCCACAAGT GGCCAGTGCC ATGAAACTGA ATCCACAGCA AGCCCCATTA 
TATGGAGAGT GTCTGCTGAC GGTCCAGCTC TGTGAGGATG AGTGTGTTGA GGATGAGGAT 
GATGTGGAGT TTTACCTGCT CTTTGCTGGA TCCACACAGA GACATGTGTC CAGCACACTG 
AAGATCAGCC ATGTCACGCT GCAGGCCGTC TGTCCAGCTC ATGATCGCAG TGAAACGGTA 
CGGGTCACAC TATGCCAGGC TCGTCCCGGC GGCTCAGTGG ACCCAGTGTC CGAGGAGTGT 
TTCCAGTTTG TCCAGGACCT GGCTCTGGAC ATGGCCCAGT TCCTGGTAAA TGCGGCGGGG 
AAAACAGAAG GAGCGCTGCT GCTGGACGAG TGTCAGATCC CACTGAAAGA GTGCGAGCGG 
CTGGACGATA CTCTTGCTCT GGCTCTCCGG CATCTGCCTT TGCCTGCTGG ATGGAGCGTT 
TTGGGCACAG ATTTGGACAC GGGCACTTGC ACAGGCTCGG GCATCGCAGA GCCTGGACCA 
CACGATACAC TTTTGCACTT CGCTGCGAAG CGCGGACTAC GCAAGGTTGC ACTCTTTCTG 
CTCCAGCAAC CAGGGGGAAG AGAGGCTCTC CGGCTTCCCA ACAAACAGGG TCGCACACCG 
GCCAAGGTCG CCCAGAAGAG GGGACACTCT CAGCTCCACA AACTGCTCAC GGAGGTTGAC 
AATCTGCCCG AGCTGGAGAC GAAAACATCT AAGTGGTGTT ACCCAGAAGG CAGAGTCCTG 
CAACACCACC CCAAGCTAAA CACTTACACC CTTACCCTGG AGGATGTGCC CAGGAGCTCC 
CCACCAAGCC TTCAGTGTGA TGTGGAGGAG CTCCAGCGTC TCATTCAGTC ACATCAGAAG 
GAAGATCCTC CAACACCGCA TAAGTCAGCA CTGCTGATTC TCAATAGAGA CTCCAGTAAT 
AGCACAGAGA CAGATGACTG CCTTCCGCCC AACTCAGTGG AACCTGAGCA TCAGGAGCAC 
AACAAACAAG AAGAAACTTG CAAATCGCTT TTAAACAGCC TTACCTTTGA GGATGGATTG 
TGTGTTCATC AAAACGGCAC AGGAGATCAT GTCCAGTTGC TGGATGGGGA CGTGGAGCCT 
GACCTGCCAG CTGTAGGAGA TACAGGGAAT GCATTAGATA AGACTGACTG TTTGCAGCGG 
CGGGTAGATG GTGATTCTGT GGCTTTCAGT GCCCCTTCCT GTGGAAACCA GCGGGAGGAA 
GCCGATATAG AGCTCTGCAT AATCAGTGCC AAGCAGGGGG TTGCTTCACA GCCTGATAAA 
GGGAAGCAAG AAGAGCAAGA GGCTTGCCAG AAGAGGTCAG AGACTGAAAA GGACTATAAC 
ACTGAATCTG GCAACCTTGG GCTTCAAGAC ATGGGCCACG CACAATCTCA GGGTCTCCTC 
CCCACGGAAA CTGGTCAGCC TACACAAAGG GAGGTGGAGA GTGAGGGGAG TACAGATGTC 
TGTTGGCGTG GGGACTTACC AGAGGAAAGG GAAGGGGACA TGAGTAGCAA CCAGGAAATA 
GACTCATATT CTTCAGCACT TTCTTCCCAT AATCTAGAGG GAAACAGCCA GCACGATGGT 
GAGACTGAGC CCCAGTTGCC TACACAATGC TCAGAGGGAA CAGATGAAGC TAAATTGACT 
TTAATTCAGG TAAATTCTGA AGTTACTTTG TCTATACGAA GCGAGGATAC CGTTATTTCT 
GACAGTGTTG ACAAGCCACA TGGTCTCTTA GAGACGGAGA CAGGAAATCT CAGCGAACAC 
ACCAAAGGCA ACACTGAGTC AGCTGCTGAT TTGGTTGCGG ATGACGCTAG TGTAAAGCAG 
AACTCTGGAT TTAGCGTTTT AGAGGGGGAT CTTTTGGAAT CATTAAGTCT ACGGCCAAAC 
ATGGCACCGG GACCAGTGCA TAAGGTTCAG CAAGGTCTGT CACCAGTTCC TGAGACTTCC 
TCTCATGTAG GTCCAGCTTT GGACCCCTTT TCTGAGACTA CAGAAAGTTT TCAACAGCCG 
AATCAATCCA CACAGCTTTT AGAAACTGCA TTAGAATGTG AGGATGTTTC ATTAAATAAC 
TCAACCGGTG ACATTAATGC AATAGCAGGG GTTTCTGTTG ACATTTCTGC AGAGGTTTCA 
CTTTGCCAGA ATGAAGGGTG TCCTCCTGAT GGAAGCAAAG AAGAGGTAGC CCCCAGTGAG 
AGGATACCAG ACCTTAAAGA GCTTACGGAA GAGCCGGAAT CAGTTGAGGA CATTATTGTC 
TCAGATGTCT CAGATTTGGA TGACAAATCT CAACCTTTTG AAGAGTCTTC TTTGCCTATC 
GACTCTGAAT GTTTTATAAG AGGTGATTTC CAAAACGATG AGTCTACAGT ACAGTCTTCT 
GAATGTACTG GAGGTGACAT AAATGTAGGA ACACTCTTAG GAGAAGAAGA GGCAACTGAA 
AAAATAGAAC AGCCAGACTC TTCACTTAAA CTGGTTGAGC TAATTCCTGA GGAATATACT 
GGAGATGCCA CAAACGAAAA TAAAAAAATT GAGCCAGAGG AACAATCTAT TTTACTGCCA 
ACTTTGCAAG GGGAATCAGG ATTTAAGGAT GTTGCCACAG AGGAACCTGT ACAGGAAAGG 
GAAATTGACT CCACTTCAGA GGAAACAATC AGCGATCTCC CTGATCCATC GGAAATCACA 
AACAATGATT GTGCTGAATC CAATGCACAC TGCCAGCTCT CAGAATCCTC CAAGGAGAGC 
GCAAACACAT CCTCAGAACC TGCTGAATGT GTGAATGACC TAGACTTGTC TTGTGCTTTA 
CCTCAAACAG AAATCGAAGG CAGCAGACCT TCTGAGGGCC CCTTAATTGA TGGAGTAACA 
ATATGTGCTG AAATTCCCAT TGATGACAAA GGTGTTGACA TGGCTACCAA TATGAGTAAG 
ACGACCATCT CTGAGGATTG TCTGTCCTCT CCTATGGACA CCACCAGCTG TTCAGTTCTG 
GAGAGCTCCT CCATGTCTGA CCCACTCAAT GCCTCATCCT CAACAGCTGA GCTCACTTCC 
CCTTTGGATA TTAATAGTGG ATTGCATCAG GATTTGGAAC ACACTTCCCC AGTGGATATA 
GACAATGGTT TGCCTGAGGA ACCGCCGCCG TCAGACACAC CTGATTGCCT GAATGGATTA 
CATCAAGATT CTCCTGATAG TTTGATGGAG CACAGTGGAA CTCCTCAGCC ATGTTCTGAG 
GAACAGTGTG TGGATCAAAT CACAGATGAC AGCACTTCAG CAGAGGATGC AGCGGACGGA 
CACCAAGACT CCTCAGTAGA CATAGCAAAT TCCGAAGCGA TCCACAGAGA GACCGAAACA 
TTTTATTCAG TTGATGCCAG TCAAGACTCT GTACTCCAGG AGAAGAAATC TTCTTCAGTG 
AGCTCAGAAA TCCCCTCTTT GACCCCCTCA CCTGAGAGCA TACGTCACAA AGAGTCGCCA 
AGCACTATGC GAGGGTCCGC CAGCGATGGA GACTCTCTGT TTAGTCAGGA TCTGGGCGAG 
GACAGCGTTT TTAATAATAA GGCTGAGGAC AGCATTACCA TCACCAGTGG CGTGTCCATG 
TCCTATTCGT CCTCCACAGA TGACTCTTCC AGCTTGGGAA CCACTTGCGC CACCCCTCAG 
ACTGGCACAG AGGCTCCTGC ATCAGACCCC TTTCAGGAAC AGCCCACATC TACTGGAGCT 
TCTGGGGAAA CAGAGGAGGA AGAGAAAAAG GACCGTCTCA CTGAGGTTCC GGAGCGATCG 
GCCATTTTAC GGACCACCAT ACGATCACTG TCCCCTTTTC GAAGACACAG TTGGGGGCCG 
GGCAAAAATT TGGCAGGGGA GACAGAGATG AGTCAGCGCA GTTACAGTCT GGAAGGACTT 
GCACCAGAGA AGGAGGGAAG GAAAAGTCTG GTTCAGCAGC GTGTCGGAGA GTCCAGCCAG 
GAGCCCAAAT GTCCATCAAA GGTGGAAAAA GAAGACAGAG GCTCGCTTGT GTCTCTGACT 
GAAGAAGAGC AGGAGTCAGA TCTAGGCGAC TGCAGCAGTT TGGGCAGTCA GAAGTCCATC 
CAGTCGGGTC GGAGATGTGT CCCACATTCA CAACCCATCC TCACCAAATC TGTGTCCATG 
GTGGCCATCA GTCAGAAGGA CCTGGACGCA ATGGGACGGC TGCGACCCAA GCGACGAATC 
TCCTTCTCCT TCAGCATCTC GCCGATGCTC CCCAAATCTA AAACTCTCTT TTCTCTTGGC 
TCTTCATCCA GTGATGAGGA GGAGGCAGTC AGATTGAGTT CATTCACTGG AACATCAGGA 
TCATTAGAGT ACAGTATATC AGAGGAGGAT CCTGGGCCAT TACGGAGTGA CAGTGAAGGC 
AAAATTGGAG GAACCAAAGT GAGCAGGACA TTCAGTTACC TCAAGAGCAA GATGAGCAAG 
AAGAACAAGC AGGAGAAGGA GAAAGAGAAG AATAAGGAGC GTGAGCGAGA TGCCAAAGAC 
AAGGAGAGAA GAGCCTCAAA CGGACATCTC TTCACTACGG TCATGACTGT ACCCACAATC 
CCCTGCCACC AGTGCAACAA ACTCATCAAC ACTAAAGACT CTATCCTCTG CACCAATTGC 
AATGCACAAG TCCACAAAGG CTGCAAAGAG AGTCTTCCTG TCTGCGCTAA AGTCAAGATG 
AAGCAGCAAA AACAGCAGCA GACTGTTACA GATTCTGCCT CGACGCCTAG TGTCACCCTC 
AGAAGTAAAA CTCTTCCAGC AAGGGATCGT CCGTGGTCCA CAATATCCAT TCCCGATGAC 
CAGCCGGCTC CCCTCGCTCC TCCTCGGAAA AGCCCCAGCA GCATCATGTC TTTCAACAGC 
AACCCACTGT CCAAGAGCAT GTCCATTAAT AACATAGCAG GACAAATGGA CGACACTCCT 
CTTAAGAAGT TTCTGTCCCA GTCAACGGAC TCTCTTCACA AGACCAGTAA AGTCACGGAG 
TCTACAGAAT CTCTTACTGA TGAGGGTACA GAGATGATGG ACAGCCAGCT GATGGGAGAA 
TTTGAAGCTG ACGCCAAAGA GCTGGAGGCT GATTCTTATA GCTTCACGGT AGACAAGAAA 
TTTCTTAAGC AGCTTAAAAA GGATGTTATC AAAAGGCAAG ACGTCATCTA TGAGCTCATC 
CAGACAGAAA TGCACCATGT AAGGACCTTA AAGATCATGG CTGATGTTTA CAGCAAAGGA 
CTACTGAGAG AAGTTCAACT AGAAGCTCAG ACGGTGGAGA AGATGTTCCC CATGTTGGAC 
GATGTTCTGG ATTTGCACAC ACAGTTCTTT TCACACCTGC TTGAAAGAAA GAAGGAATCA 
GGGAGCCAAG AAGAGGGCGG CTTTGTCATC CAAAGGATTG GCGATGTGCT GGTTTCTCAG 
TTCTCAGGTT CCAGTGCTGA GAGAATGAAG AAGGTCTATG GGAAGTTCTG CGGCAGGCAC 
AATGAAGCTG TGAACTTCTA CAAGGAGCTT CACACCAAAG ACAAACGCTT TCAAGCTTTC 
ATCAAGAAAA AGATGAGCAG CCCTGTTGTC CGACGGCTAA GCATTCCTGA GTGTATATTG 
TTAGTCACAC AACGAATCAC AAAATATCCC GTGTTACTAC AGAGAATACT GCAGCACACG 
AAAGAAAACG AGGAGGACCA TGCAGACGTT AGTCAAGGTC TGAAGCTGGT GAAGGAGGTG 
ATTGCTGCCG TGGACAATAA AGTGAATGAG CATGAGAAGA AGAAGAGGCT AAAGGAAGTG 
TACAGCCGGA CGGACAACAA GTCCATCATG AGGATGAAGA GCGGGCAGAT GTTTGCCAGA 
GAGGATCTGT TACGTGGGCG GAAGTTAATT CACGACGGGC CGCTGCAGCT CAAGAACTCA 
GCTGGCAGAC TCAAGGATGT CCAGGCTCTG CTGCTGAGGG ACGTGATTGT GTTCCTGCAG 
GAGAAAGATC AGAAGTACAT TTTCGCCTCT TTGGACCAGC GCTCCACCGT CATCTCCCTG 
CAGAAGCTCA TTGTGAGGGA AGTAGCCAAT GAGGAGAAAG CTCTGTTTCT GATCACAGCA 
GGCATCGAGA AGCCCGAGAT GGTAGAAGTG TACACAAGCT CCAAAGAGGA GAGAAACACA 
TGGATGCAGC TCATTCAGGA GGCCATGCAG TCTATACTGT CCATTTCCTC CAGGGAGAGA 
GATGATGATG AAGGTGTGCC AAGTGAAAAC GAAGAGGACA GGAGACTTCA GGAAATCAAG 
GTCAAGGAGT TCAGAGATCA GCTGCACAAA AGAGACGAGC AGATCTTGAC GTTACTGGAG 
GAGAAGGTGA AGTTGTTCAG GGACATGTGT GACTGTGGTG CACCGGACGA GACGGGGCTT 
CGTAATCGAA TGCTCTTCAG GGCCACGACG GATGATGTCA CCAGAGGAGA GCCAATCATG 
AAGGAGGCTC TGAAGGAAGT TGAAACGCTG CAGGTTCTGG TTAATGACAG TCTTGGCGGT 
GCGGTTCAGG AAGGTGTGTG CGCGGCTGGA CATGTGACTT TGCCACGACG AGCGGAAACA 
TTTGGAGGTT TCGACAGTCA CCAGATGAAC ATCTCTAAGA GCAAAGATGG AGACAGAGAG 
GAGCTGGAGG AGTCTGCAGA GCTCCGCAGG ACTGAGTCAG ACAGTGTGTT GAAGAAGACG 
AGTAAGACCA ACCCTTTACT GCTGGTCAGG AGAAACAATG AGCAGGTTCT TCAGAGCGTT 
GCTCATCTTC ATGACCTGCT GAACACATTA CAAGCTGTGG TGGTCCAGCA GGACACCTTC 
ATCGAAGACC AGCGTCACGC TCATCCCTCC TCACGCCACC CATCCATCTC CTCCTCTACC 
TCCTCTTTAT CATCCACCTC ATCCCGGCCG AGCTCTCTAA TCGAGCAGGA GAAACAGCGC 
AGCCTGGAGA AGCAGCGGCA GGAGGTGGCC AGCCTCCAAA AACAGCAGGC GGCCCACGCC 
GAGGAGAGGC GGCGCAGGGA AAAGCAGTGG GAGACCCGAG AGAAAGAGCT AACCCAGAGG 
GAGATGCAGA TCCAGAGCCA AGAAGAGGAG GCGCAGAGGA GAGCCAGACA GCTGGAGGAG 
GAGAAACAGG AGCTGCAGAG TAAGAAAGAG GAGTATCAGA GAGACCTGGC ACGACTGCGG 
GACAGCCAGA AGAGACTGGA GAGAGAGAGA GAGCAGGTGC AGAGAGAGGC AGATGAGCTT 
AAACTGACTG AGGAAGTGAC AAAGAACCGG TCTCCGTCAA CCACGTCTGA AGACTCCACG 
AAGTTCCAGA GCACCGGTTC ATTAGACCGT GACCCATCAG AGATGGAGCT CTCCTCCTCC 
CCGTCAGCCA GAGACTTCCT GTCCCGCATG GACTCCAAAC GCAAAGGGAA AAACCTCAAT 
CCATTCTCAT CCAACTCAAG CCAGAAGAGC CAGAACAGCG AGGCTCAGAG CCAGATGCCC 
AGCCGTCTGC TCCAGCTGGC CAAACCCAAG GAGAAGAAGG ACAAGAAGAA GAAGAAGGGG 
AAAGGCCAGC AGAGCCAGAC TGCAGACTCT CAGAGCACTG TTGTTCCAGG CGCTACCCCA 
GACGGAGAGA TCTTTTTCTG CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

wu:fi15d04 ( XM_009297842.2 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_009296117.1 Danio rerio wu:fi15d04 (wu:fi15d04), transcript variant X9, mRNA.

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CloneID	ODa39936
Clone ID Related Accession (Same CDS sequence)	XM_009297842.2
Accession Version	XM_009297842.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7569bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-23
Organism	Danio rerio(Zebrafish)
Product	A-kinase anchor protein 13 isoform X9
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007136.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_009297842.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAGGGTG TTCCACAAGT GGCCAGTGCC ATGAAACTGA ATCCACAGCA AGCCCCATTA 
TATGGAGAGT GTCTGCTGAC GGTCCAGCTC TGTGAGGATG AGTGTGTTGA GGATGAGGAT 
GATGTGGAGT TTTACCTGCT CTTTGCTGGA TCCACACAGA GACATGTGTC CAGCACACTG 
AAGATCAGCC ATGTCACGCT GCAGGCCGTC TGTCCAGCTC ATGATCGCAG TGAAACGGTA 
CGGGTCACAC TATGCCAGGC TCGTCCCGGC GGCTCAGTGG ACCCAGTGTC CGAGGAGTGT 
TTCCAGTTTG TCCAGGACCT GGCTCTGGAC ATGGCCCAGT TCCTGGTAAA TGCGGCGGGG 
AAAACAGAAG GAGCGCTGCT GCTGGACGAG TGTCAGATCC CACTGAAAGA GTGCGAGCGG 
CTGGACGATA CTCTTGCTCT GGCTCTCCGG CATCTGCCTT TGCCTGCTGG ATGGAGCGTT 
TTGGGCACAG ATTTGGACAC GGGCACTTGC ACAGGCTCGG GCATCGCAGA GCCTGGACCA 
CACGATACAC TTTTGCACTT CGCTGCGAAG CGCGGACTAC GCAAGGTTGC ACTCTTTCTG 
CTCCAGCAAC CAGGGGGAAG AGAGGCTCTC CGGCTTCCCA ACAAACAGGG TCGCACACCG 
GCCAAGGTCG CCCAGAAGAG GGGACACTCT CAGCTCCACA AACTGCTCAC GGAGGTTGAC 
AATCTGCCCG AGCTGGAGAC GAAAACATCT AAGTGGTGTT ACCCAGAAGG CAGAGTCCTG 
CAACACCACC CCAAGCTAAA CACTTACACC CTTACCCTGG AGGATGTGCC CAGGAGCTCC 
CCACCAAGCC TTCAGTGTGA TGTGGAGGAG CTCCAGCGTC TCATTCAGTC ACATCAGAAG 
GAAGATCCTC CAACACCGCA TAAGTCAGCA CTGCTGATTC TCAATAGAGA CTCCAGTAAT 
AGCACAGAGA CAGATGACTG CCTTCCGCCC AACTCAGTGG AACCTGAGCA TCAGGAGCAC 
AACAAACAAG AAGAAACTTG CAAATCGCTT TTAAACAGCC TTACCTTTGA GGATGGATTG 
TGTGTTCATC AAAACGGCAC AGGAGATCAT GTCCAGTTGC TGGATGGGGA CGTGGAGCCT 
GACCTGCCAG CTGTAGGAGA TACAGGGAAT GCATTAGATA AGACTGACTG TTTGCAGCGG 
CGGGTAGATG GTGATTCTGT GGCTTTCAGT GCCCCTTCCT GTGGAAACCA GCGGGAGGAA 
GCCGATATAG AGCTCTGCAT AATCAGTGCC AAGCAGGGGG TTGCTTCACA GCCTGATAAA 
GGGAAGCAAG AAGAGCAAGA GGCTTGCCAG AAGAGGTCAG AGACTGAAAA GGACTATAAC 
ACTGAATCTG GCAACCTTGG GCTTCAAGAC ATGGGCCACG CACAATCTCA GGGTCTCCTC 
CCCACGGAAA CTGGTCAGCC TACACAAAGG GAGGTGGAGA GTGAGGGGAG TACAGATGTC 
TGTTGGCGTG GGGACTTACC AGAGGAAAGG GAAGGGGACA TGAGTAGCAA CCAGGAAATA 
GACTCATATT CTTCAGCACT TTCTTCCCAT AATCTAGAGG GAAACAGCCA GCACGATGGT 
GAGACTGAGC CCCAGTTGCC TACACAATGC TCAGAGGGAA CAGATGAAGC TAAATTGACT 
TTAATTCAGG TAAATTCTGA AGTTACTTTG TCTATACGAA GCGAGGATAC CGTTATTTCT 
GACAGTGTTG ACAAGCCACA TGGTCTCTTA GAGACGGAGA CAGGAAATCT CAGCGAACAC 
ACCAAAGGCA ACACTGAGTC AGCTGCTGAT TTGGTTGCGG ATGACGCTAG TGTAAAGCAG 
AACTCTGGAT TTAGCGTTTT AGAGGGGGAT CTTTTGGAAT CATTAAGTCT ACGGCCAAAC 
ATGGCACCGG GACCAGTGCA TAAGGTTCAG CAAGGTCTGT CACCAGTTCC TGAGACTTCC 
TCTCATGTAG GTCCAGCTTT GGACCCCTTT TCTGAGACTA CAGAAAGTTT TCAACAGCCG 
AATCAATCCA CACAGCTTTT AGAAACTGCA TTAGAATGTG AGGATGTTTC ATTAAATAAC 
TCAACCGGTG ACATTAATGC AATAGCAGGG GTTTCTGTTG ACATTTCTGC AGAGGTTTCA 
CTTTGCCAGA ATGAAGGGTG TCCTCCTGAT GGAAGCAAAG AAGAGGTAGC CCCCAGTGAG 
AGGATACCAG ACCTTAAAGA GCTTACGGAA GAGCCGGAAT CAGTTGAGGA CATTATTGTC 
TCAGATGTCT CAGATTTGGA TGACAAATCT CAACCTTTTG AAGAGTCTTC TTTGCCTATC 
GACTCTGAAT GTTTTATAAG AGGTGATTTC CAAAACGATG AGTCTACAGT ACAGTCTTCT 
GAATGTACTG GAGGTGACAT AAATGTAGGA ACACTCTTAG GAGAAGAAGA GGCAACTGAA 
AAAATAGAAC AGCCAGACTC TTCACTTAAA CTGGTTGAGC TAATTCCTGA GGAATATACT 
GGAGATGCCA CAAACGAAAA TAAAAAAATT GAGCCAGAGG AACAATCTAT TTTACTGCCA 
ACTTTGCAAG GGGAATCAGG ATTTAAGGAT GTTGCCACAG AGGAACCTGT ACAGGAAAGG 
GAAATTGACT CCACTTCAGA GGAAACAATC AGCGATCTCC CTGATCCATC GGAAATCACA 
AACAATGATT GTGCTGAATC CAATGCACAC TGCCAGCTCT CAGAATCCTC CAAGGAGAGC 
GCAAACACAT CCTCAGAACC TGCTGAATGT GTGAATGACC TAGACTTGTC TTGTGCTTTA 
CCTCAAACAG AAATCGAAGG CAGCAGACCT TCTGAGGGCC CCTTAATTGA TGGAGTAACA 
ATATGTGCTG AAATTCCCAT TGATGACAAA GGTGTTGACA TGGCTACCAA TATGAGTAAG 
ACGACCATCT CTGAGGATTG TCTGTCCTCT CCTATGGACA CCACCAGCTG TTCAGTTCTG 
GAGAGCTCCT CCATGTCTGA CCCACTCAAT GCCTCATCCT CAACAGCTGA GCTCACTTCC 
CCTTTGGATA TTAATAGTGG ATTGCATCAG GATTTGGAAC ACACTTCCCC AGTGGATATA 
GACAATGGTT TGCCTGAGGA ACCGCCGCCG TCAGACACAC CTGATTGCCT GAATGGATTA 
CATCAAGATT CTCCTGATAG TTTGATGGAG CACAGTGGAA CTCCTCAGCC ATGTTCTGAG 
GAACAGTGTG TGGATCAAAT CACAGATGAC AGCACTTCAG CAGAGGATGC AGCGGACGGA 
CACCAAGACT CCTCAGTAGA CATAGCAAAT TCCGAAGCGA TCCACAGAGA GACCGAAACA 
TTTTATTCAG TTGATGCCAG TCAAGACTCT GTACTCCAGG AGAAGAAATC TTCTTCAGTG 
AGCTCAGAAA TCCCCTCTTT GACCCCCTCA CCTGAGAGCA TACGTCACAA AGAGTCGCCA 
AGCACTATGC GAGGGTCCGC CAGCGATGGA GACTCTCTGT TTAGTCAGGA TCTGGGCGAG 
GACAGCGTTT TTAATAATAA GGCTGAGGAC AGCATTACCA TCACCAGTGG CGTGTCCATG 
TCCTATTCGT CCTCCACAGA TGACTCTTCC AGCTTGGGAA CCACTTGCGC CACCCCTCAG 
ACTGGCACAG AGGCTCCTGC ATCAGACCCC TTTCAGGAAC AGCCCACATC TACTGGAGCT 
TCTGGGGAAA CAGAGGAGGA AGAGAAAAAG GACCGTCTCA CTGAGGTTCC GGAGCGATCG 
GCCATTTTAC GGACCACCAT ACGATCACTG TCCCCTTTTC GAAGACACAG TTGGGGGCCG 
GGCAAAAATT TGGCAGGGGA GACAGAGATG AGTCAGCGCA GTGCGGCACA AGGTGAAGTG 
GAGCGCAGAT CTGTTGGTCA TAGGAGAAGC ATGAGCTGGT GTCCTTCTGT TGTCCTGCGC 
TCTGAAAATG ATGAAATGAA TGAAAGAAGT TACAGTCTGG AAGGACTTGC ACCAGAGAAG 
GAGGGAAGGA AAAGTCTGGT TCAGCAGCGT GTCGGAGAGT CCAGCCAGGA GCCCAAATGT 
CCATCAAAGG TGGAAAAAGA AGACAGAGGC TCGCTTGTGT CTCTGACTGA AGAAGAGCAG 
GAGTCAGATC TAGGCGACTG CAGCAGTTTG GGCAGTCAGA AGTCCATCCA GTCGGGTCGG 
AGATGTGTCC CACATTCACA ACCCATCCTC ACCAAATCTG TGTCCATGGT GGCCATCAGT 
CAGAAGGACC TGGACGGATT GAGTTCATTC ACTGGAACAT CAGGATCATT AGAGTACAGT 
ATATCAGAGG AGGATCCTGG GCCATTACGG AGTGACAGTG AAGGCAAAAT TGGAGGAACC 
AAAGTGAGCA GGACATTCAG TTACCTCAAG AGCAAGATGA GCAAGAAGAA CAAGCAGGAG 
AAGGAGAAAG AGAAGAATAA GGAGCGTGAG CGAGATGCCA AAGACAAGGA GAGAAGAGCC 
TCAAACGGAC ATCTCTTCAC TACGGTCATG ACTGTACCCA CAATCCCCTG CCACCAGTGC 
AACAAACTCA TCAACACTAA AGACTCTATC CTCTGCACCA ATTGCAATGC ACAAGTCCAC 
AAAGGCTGCA AAGAGAGTCT TCCTGTCTGC GCTAAAGTCA AGATGAAGCA GCAAAAACAG 
CAGCAGACTG TTACAGATTC TGCCTCGACG CCTAGTGTCA CCCTCAGAAG TAAAACTCTT 
CCAGCAAGGG ATCGTCCGTG GTCCACAATA TCCATTCCCG ATGACCAGCC GGCTCCCCTC 
GCTCCTCCTC GGAAAAGCCC CAGCAGCATC ATGTCTTTCA ACAGCAACCC ACTGTCCAAG 
AGCATGTCCA TTAATAACAT AGCAGGACAA ATGGACGACA CTCCTCTTAA GAAGTTTCTG 
TCCCAGTCAA CGGACTCTCT TCACAAGACC AGTAAAGTCA CGGAGTCTAC AGAATCTCTT 
ACTGATGAGG GTACAGAGAT GATGGACAGC CAGCTGATGG GAGAATTTGA AGCTGACGCC 
AAAGAGCTGG AGGCTGATTC TTATAGCTTC ACGGTAGACA AGAAATTTCT TAAGCAGCTT 
AAAAAGGATG TTATCAAAAG GCAAGACGTC ATCTATGAGC TCATCCAGAC AGAAATGCAC 
CATGTAAGGA CCTTAAAGAT CATGGCTGAT GTTTACAGCA AAGGACTACT GAGAGAAGTT 
CAACTAGAAG CTCAGACGGT GGAGAAGATG TTCCCCATGT TGGACGATGT TCTGGATTTG 
CACACACAGT TCTTTTCACA CCTGCTTGAA AGAAAGAAGG AATCAGGGAG CCAAGAAGAG 
GGCGGCTTTG TCATCCAAAG GATTGGCGAT GTGCTGGTTT CTCAGTTCTC AGGTTCCAGT 
GCTGAGAGAA TGAAGAAGGT CTATGGGAAG TTCTGCGGCA GGCACAATGA AGCTGTGAAC 
TTCTACAAGG AGCTTCACAC CAAAGACAAA CGCTTTCAAG CTTTCATCAA GAAAAAGATG 
AGCAGCCCTG TTGTCCGACG GCTAAGCATT CCTGAGTGTA TATTGTTAGT CACACAACGA 
ATCACAAAAT ATCCCGTGTT ACTACAGAGA ATACTGCAGC ACACGAAAGA AAACGAGGAG 
GACCATGCAG ACGTTAGTCA AGGTCTGAAG CTGGTGAAGG AGGTGATTGC TGCCGTGGAC 
AATAAAGTGA ATGAGCATGA GAAGAAGAAG AGGCTAAAGG AAGTGTACAG CCGGACGGAC 
AACAAGTCCA TCATGAGGAT GAAGAGCGGG CAGATGTTTG CCAGAGAGGA TCTGTTACGT 
GGGCGGAAGT TAATTCACGA CGGGCCGCTG CAGCTCAAGA ACTCAGCTGG CAGACTCAAG 
GATGTCCAGG CTCTGCTGCT GAGGGACGTG ATTGTGTTCC TGCAGGAGAA AGATCAGAAG 
TACATTTTCG CCTCTTTGGA CCAGCGCTCC ACCGTCATCT CCCTGCAGAA GCTCATTGTG 
AGGGAAGTAG CCAATGAGGA GAAAGCTCTG TTTCTGATCA CAGCAGGCAT CGAGAAGCCC 
GAGATGGTAG AAGTGTACAC AAGCTCCAAA GAGGAGAGAA ACACATGGAT GCAGCTCATT 
CAGGAGGCCA TGCAGTCTAT ACTGTCCATT TCCTCCAGGG AGAGAGATGA TGATGAAGGT 
GTGCCAAGTG AAAACGAAGA GGACAGGAGA CTTCAGGAAA TCAAGGTCAA GGAGTTCAGA 
GATCAGCTGC ACAAAAGAGA CGAGCAGATC TTGACGTTAC TGGAGGAGAA GGTGAAGTTG 
TTCAGGGACA TGTGTGACTG TGGTGCACCG GACGAGACGG GGCTTCGTAA TCGAATGCTC 
TTCAGGGCCA CGACGGATGA TGTCACCAGA GGAGAGCCAA TCATGAAGGA GGCTCTGAAG 
GAAGTTGAAA CGCTGCAGGT TCTGGTTAAT GACAGTCTTG GCGGTGCGGT TCAGGAAGGT 
GTGTGCGCGG CTGGACATGT GACTTTGCCA CGACGAGCGG AAACATTTGG AGGTTTCGAC 
AGTCACCAGA TGAACATCTC TAAGAGCAAA GATGGAGACA GAGAGGAGCT GGAGGAGTCT 
GCAGAGCTCC GCAGGACTGA GTCAGACAGT GTGTTGAAGA AGACGAGTAA GACCAACCCT 
TTACTGCTGG TCAGGAGAAA CAATGAGCAG GTTCTTCAGA GCGTTGCTCA TCTTCATGAC 
CTGCTGAACA CATTACAAGC TGTGGTGGTC CAGCAGGACA CCTTCATCGA AGACCAGCGT 
CACGCTCATC CCTCCTCACG CCACCCATCC ATCTCCTCCT CTACCTCCTC TTTATCATCC 
ACCTCATCCC GGCCGAGCTC TCTAATCGAG CAGGAGAAAC AGCGCAGCCT GGAGAAGCAG 
CGGCAGGAGG TGGCCAGCCT CCAAAAACAG CAGGCGGCCC ACGCCGAGGA GAGGCGGCGC 
AGGGAAAAGC AGTGGGAGAC CCGAGAGAAA GAGCTAACCC AGAGGGAGAT GCAGATCCAG 
AGCCAAGAAG AGGAGGCGCA GAGGAGAGCC AGACAGCTGG AGGAGGAGAA ACAGGAGCTG 
CAGAGTAAGA AAGAGGAGTA TCAGAGAGAC CTGGCACGAC TGCGGGACAG CCAGAAGAGA 
CTGGAGAGAG AGAGAGAGCA GGTGCAGAGA GAGGCAGATG AGCTTAAACT GACTGAGGAA 
GTGACAAAGA ACCGGTCTCC GTCAACCACG TCTGAAGACT CCACGAAGTT CCAGAGCACC 
GGTTCATTAG ACCGTGACCC ATCAGAGATG GAGCTCTCCT CCTCCCCGTC AGCCAGAGAC 
TTCCTGTCCC GCATGGACTC CAAACGCAAA GGGAAAAACC TCAATCCATT CTCATCCAAC 
TCAAGCCAGA AGAGCCAGAA CAGCGAGGCT CAGAGCCAGA TGCCCAGCCG TCTGCTCCAG 
CTGGCCAAAC CCAAGGAGAA GAAGGACAAG AAGAAGAAGA AGGGGAAAGG CCAGCAGAGC 
CAGACTGCAG ACTCTCAGAG CACTGTTGTT CCAGGCGCTA CCCCAGACGG AGAGATCTTT 
TTCTGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_009296117.1
CDS	28..7596
Translation	MKGVPQVASAMKLNPQQAPLYGECLLTVQLCEDECVEDEDDVEFYLLFAGSTQRHVSSTLKISHVTLQAVCPAHDRSETVRVTLCQARPGGSVDPVSEECFQFVQDLALDMAQFLVNAAGKTEGALLLDECQIPLKECERLDDTLALALRHLPLPAGWSVLGTDLDTGTCTGSGIAEPGPHDTLLHFAAKRGLRKVALFLLQQPGGREALRLPNKQGRTPAKVAQKRGHSQLHKLLTEVDNLPELETKTSKWCYPEGRVLQHHPKLNTYTLTLEDVPRSSPPSLQCDVEELQRLIQSHQKEDPPTPHKSALLILNRDSSNSTETDDCLPPNSVEPEHQEHNKQEETCKSLLNSLTFEDGLCVHQNGTGDHVQLLDGDVEPDLPAVGDTGNALDKTDCLQRRVDGDSVAFSAPSCGNQREEADIELCIISAKQGVASQPDKGKQEEQEACQKRSETEKDYNTESGNLGLQDMGHAQSQGLLPTETGQPTQREVESEGSTDVCWRGDLPEEREGDMSSNQEIDSYSSALSSHNLEGNSQHDGETEPQLPTQCSEGTDEAKLTLIQVNSEVTLSIRSEDTVISDSVDKPHGLLETETGNLSEHTKGNTESAADLVADDASVKQNSGFSVLEGDLLESLSLRPNMAPGPVHKVQQGLSPVPETSSHVGPALDPFSETTESFQQPNQSTQLLETALECEDVSLNNSTGDINAIAGVSVDISAEVSLCQNEGCPPDGSKEEVAPSERIPDLKELTEEPESVEDIIVSDVSDLDDKSQPFEESSLPIDSECFIRGDFQNDESTVQSSECTGGDINVGTLLGEEEATEKIEQPDSSLKLVELIPEEYTGDATNENKKIEPEEQSILLPTLQGESGFKDVATEEPVQEREIDSTSEETISDLPDPSEITNNDCAESNAHCQLSESSKESANTSSEPAECVNDLDLSCALPQTEIEGSRPSEGPLIDGVTICAEIPIDDKGVDMATNMSKTTISEDCLSSPMDTTSCSVLESSSMSDPLNASSSTAELTSPLDINSGLHQDLEHTSPVDIDNGLPEEPPPSDTPDCLNGLHQDSPDSLMEHSGTPQPCSEEQCVDQITDDSTSAEDAADGHQDSSVDIANSEAIHRETETFYSVDASQDSVLQEKKSSSVSSEIPSLTPSPESIRHKESPSTMRGSASDGDSLFSQDLGEDSVFNNKAEDSITITSGVSMSYSSSTDDSSSLGTTCATPQTGTEAPASDPFQEQPTSTGASGETEEEEKKDRLTEVPERSAILRTTIRSLSPFRRHSWGPGKNLAGETEMSQRSAAQGEVERRSVGHRRSMSWCPSVVLRSENDEMNERSYSLEGLAPEKEGRKSLVQQRVGESSQEPKCPSKVEKEDRGSLVSLTEEEQESDLGDCSSLGSQKSIQSGRRCVPHSQPILTKSVSMVAISQKDLDGLSSFTGTSGSLEYSISEEDPGPLRSDSEGKIGGTKVSRTFSYLKSKMSKKNKQEKEKEKNKERERDAKDKERRASNGHLFTTVMTVPTIPCHQCNKLINTKDSILCTNCNAQVHKGCKESLPVCAKVKMKQQKQQQTVTDSASTPSVTLRSKTLPARDRPWSTISIPDDQPAPLAPPRKSPSSIMSFNSNPLSKSMSINNIAGQMDDTPLKKFLSQSTDSLHKTSKVTESTESLTDEGTEMMDSQLMGEFEADAKELEADSYSFTVDKKFLKQLKKDVIKRQDVIYELIQTEMHHVRTLKIMADVYSKGLLREVQLEAQTVEKMFPMLDDVLDLHTQFFSHLLERKKESGSQEEGGFVIQRIGDVLVSQFSGSSAERMKKVYGKFCGRHNEAVNFYKELHTKDKRFQAFIKKKMSSPVVRRLSIPECILLVTQRITKYPVLLQRILQHTKENEEDHADVSQGLKLVKEVIAAVDNKVNEHEKKKRLKEVYSRTDNKSIMRMKSGQMFAREDLLRGRKLIHDGPLQLKNSAGRLKDVQALLLRDVIVFLQEKDQKYIFASLDQRSTVISLQKLIVREVANEEKALFLITAGIEKPEMVEVYTSSKEERNTWMQLIQEAMQSILSISSRERDDDEGVPSENEEDRRLQEIKVKEFRDQLHKRDEQILTLLEEKVKLFRDMCDCGAPDETGLRNRMLFRATTDDVTRGEPIMKEALKEVETLQVLVNDSLGGAVQEGVCAAGHVTLPRRAETFGGFDSHQMNISKSKDGDREELEESAELRRTESDSVLKKTSKTNPLLLVRRNNEQVLQSVAHLHDLLNTLQAVVVQQDTFIEDQRHAHPSSRHPSISSSTSSLSSTSSRPSSLIEQEKQRSLEKQRQEVASLQKQQAAHAEERRRREKQWETREKELTQREMQIQSQEEEAQRRARQLEEEKQELQSKKEEYQRDLARLRDSQKRLEREREQVQREADELKLTEEVTKNRSPSTTSEDSTKFQSTGSLDRDPSEMELSSSPSARDFLSRMDSKRKGKNLNPFSSNSSQKSQNSEAQSQMPSRLLQLAKPKEKKDKKKKKGKGQQSQTADSQSTVVPGATPDGEIFFC

Target ORF information:

RefSeq Version	XM_009297842.2
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio wu:fi15d04 (wu:fi15d04), transcript variant X9, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_009297842.2

ORF Insert Sequence:

ATGAAGGGTG TTCCACAAGT GGCCAGTGCC ATGAAACTGA ATCCACAGCA AGCCCCATTA 
TATGGAGAGT GTCTGCTGAC GGTCCAGCTC TGTGAGGATG AGTGTGTTGA GGATGAGGAT 
GATGTGGAGT TTTACCTGCT CTTTGCTGGA TCCACACAGA GACATGTGTC CAGCACACTG 
AAGATCAGCC ATGTCACGCT GCAGGCCGTC TGTCCAGCTC ATGATCGCAG TGAAACGGTA 
CGGGTCACAC TATGCCAGGC TCGTCCCGGC GGCTCAGTGG ACCCAGTGTC CGAGGAGTGT 
TTCCAGTTTG TCCAGGACCT GGCTCTGGAC ATGGCCCAGT TCCTGGTAAA TGCGGCGGGG 
AAAACAGAAG GAGCGCTGCT GCTGGACGAG TGTCAGATCC CACTGAAAGA GTGCGAGCGG 
CTGGACGATA CTCTTGCTCT GGCTCTCCGG CATCTGCCTT TGCCTGCTGG ATGGAGCGTT 
TTGGGCACAG ATTTGGACAC GGGCACTTGC ACAGGCTCGG GCATCGCAGA GCCTGGACCA 
CACGATACAC TTTTGCACTT CGCTGCGAAG CGCGGACTAC GCAAGGTTGC ACTCTTTCTG 
CTCCAGCAAC CAGGGGGAAG AGAGGCTCTC CGGCTTCCCA ACAAACAGGG TCGCACACCG 
GCCAAGGTCG CCCAGAAGAG GGGACACTCT CAGCTCCACA AACTGCTCAC GGAGGTTGAC 
AATCTGCCCG AGCTGGAGAC GAAAACATCT AAGTGGTGTT ACCCAGAAGG CAGAGTCCTG 
CAACACCACC CCAAGCTAAA CACTTACACC CTTACCCTGG AGGATGTGCC CAGGAGCTCC 
CCACCAAGCC TTCAGTGTGA TGTGGAGGAG CTCCAGCGTC TCATTCAGTC ACATCAGAAG 
GAAGATCCTC CAACACCGCA TAAGTCAGCA CTGCTGATTC TCAATAGAGA CTCCAGTAAT 
AGCACAGAGA CAGATGACTG CCTTCCGCCC AACTCAGTGG AACCTGAGCA TCAGGAGCAC 
AACAAACAAG AAGAAACTTG CAAATCGCTT TTAAACAGCC TTACCTTTGA GGATGGATTG 
TGTGTTCATC AAAACGGCAC AGGAGATCAT GTCCAGTTGC TGGATGGGGA CGTGGAGCCT 
GACCTGCCAG CTGTAGGAGA TACAGGGAAT GCATTAGATA AGACTGACTG TTTGCAGCGG 
CGGGTAGATG GTGATTCTGT GGCTTTCAGT GCCCCTTCCT GTGGAAACCA GCGGGAGGAA 
GCCGATATAG AGCTCTGCAT AATCAGTGCC AAGCAGGGGG TTGCTTCACA GCCTGATAAA 
GGGAAGCAAG AAGAGCAAGA GGCTTGCCAG AAGAGGTCAG AGACTGAAAA GGACTATAAC 
ACTGAATCTG GCAACCTTGG GCTTCAAGAC ATGGGCCACG CACAATCTCA GGGTCTCCTC 
CCCACGGAAA CTGGTCAGCC TACACAAAGG GAGGTGGAGA GTGAGGGGAG TACAGATGTC 
TGTTGGCGTG GGGACTTACC AGAGGAAAGG GAAGGGGACA TGAGTAGCAA CCAGGAAATA 
GACTCATATT CTTCAGCACT TTCTTCCCAT AATCTAGAGG GAAACAGCCA GCACGATGGT 
GAGACTGAGC CCCAGTTGCC TACACAATGC TCAGAGGGAA CAGATGAAGC TAAATTGACT 
TTAATTCAGG TAAATTCTGA AGTTACTTTG TCTATACGAA GCGAGGATAC CGTTATTTCT 
GACAGTGTTG ACAAGCCACA TGGTCTCTTA GAGACGGAGA CAGGAAATCT CAGCGAACAC 
ACCAAAGGCA ACACTGAGTC AGCTGCTGAT TTGGTTGCGG ATGACGCTAG TGTAAAGCAG 
AACTCTGGAT TTAGCGTTTT AGAGGGGGAT CTTTTGGAAT CATTAAGTCT ACGGCCAAAC 
ATGGCACCGG GACCAGTGCA TAAGGTTCAG CAAGGTCTGT CACCAGTTCC TGAGACTTCC 
TCTCATGTAG GTCCAGCTTT GGACCCCTTT TCTGAGACTA CAGAAAGTTT TCAACAGCCG 
AATCAATCCA CACAGCTTTT AGAAACTGCA TTAGAATGTG AGGATGTTTC ATTAAATAAC 
TCAACCGGTG ACATTAATGC AATAGCAGGG GTTTCTGTTG ACATTTCTGC AGAGGTTTCA 
CTTTGCCAGA ATGAAGGGTG TCCTCCTGAT GGAAGCAAAG AAGAGGTAGC CCCCAGTGAG 
AGGATACCAG ACCTTAAAGA GCTTACGGAA GAGCCGGAAT CAGTTGAGGA CATTATTGTC 
TCAGATGTCT CAGATTTGGA TGACAAATCT CAACCTTTTG AAGAGTCTTC TTTGCCTATC 
GACTCTGAAT GTTTTATAAG AGGTGATTTC CAAAACGATG AGTCTACAGT ACAGTCTTCT 
GAATGTACTG GAGGTGACAT AAATGTAGGA ACACTCTTAG GAGAAGAAGA GGCAACTGAA 
AAAATAGAAC AGCCAGACTC TTCACTTAAA CTGGTTGAGC TAATTCCTGA GGAATATACT 
GGAGATGCCA CAAACGAAAA TAAAAAAATT GAGCCAGAGG AACAATCTAT TTTACTGCCA 
ACTTTGCAAG GGGAATCAGG ATTTAAGGAT GTTGCCACAG AGGAACCTGT ACAGGAAAGG 
GAAATTGACT CCACTTCAGA GGAAACAATC AGCGATCTCC CTGATCCATC GGAAATCACA 
AACAATGATT GTGCTGAATC CAATGCACAC TGCCAGCTCT CAGAATCCTC CAAGGAGAGC 
GCAAACACAT CCTCAGAACC TGCTGAATGT GTGAATGACC TAGACTTGTC TTGTGCTTTA 
CCTCAAACAG AAATCGAAGG CAGCAGACCT TCTGAGGGCC CCTTAATTGA TGGAGTAACA 
ATATGTGCTG AAATTCCCAT TGATGACAAA GGTGTTGACA TGGCTACCAA TATGAGTAAG 
ACGACCATCT CTGAGGATTG TCTGTCCTCT CCTATGGACA CCACCAGCTG TTCAGTTCTG 
GAGAGCTCCT CCATGTCTGA CCCACTCAAT GCCTCATCCT CAACAGCTGA GCTCACTTCC 
CCTTTGGATA TTAATAGTGG ATTGCATCAG GATTTGGAAC ACACTTCCCC AGTGGATATA 
GACAATGGTT TGCCTGAGGA ACCGCCGCCG TCAGACACAC CTGATTGCCT GAATGGATTA 
CATCAAGATT CTCCTGATAG TTTGATGGAG CACAGTGGAA CTCCTCAGCC ATGTTCTGAG 
GAACAGTGTG TGGATCAAAT CACAGATGAC AGCACTTCAG CAGAGGATGC AGCGGACGGA 
CACCAAGACT CCTCAGTAGA CATAGCAAAT TCCGAAGCGA TCCACAGAGA GACCGAAACA 
TTTTATTCAG TTGATGCCAG TCAAGACTCT GTACTCCAGG AGAAGAAATC TTCTTCAGTG 
AGCTCAGAAA TCCCCTCTTT GACCCCCTCA CCTGAGAGCA TACGTCACAA AGAGTCGCCA 
AGCACTATGC GAGGGTCCGC CAGCGATGGA GACTCTCTGT TTAGTCAGGA TCTGGGCGAG 
GACAGCGTTT TTAATAATAA GGCTGAGGAC AGCATTACCA TCACCAGTGG CGTGTCCATG 
TCCTATTCGT CCTCCACAGA TGACTCTTCC AGCTTGGGAA CCACTTGCGC CACCCCTCAG 
ACTGGCACAG AGGCTCCTGC ATCAGACCCC TTTCAGGAAC AGCCCACATC TACTGGAGCT 
TCTGGGGAAA CAGAGGAGGA AGAGAAAAAG GACCGTCTCA CTGAGGTTCC GGAGCGATCG 
GCCATTTTAC GGACCACCAT ACGATCACTG TCCCCTTTTC GAAGACACAG TTGGGGGCCG 
GGCAAAAATT TGGCAGGGGA GACAGAGATG AGTCAGCGCA GTGCGGCACA AGGTGAAGTG 
GAGCGCAGAT CTGTTGGTCA TAGGAGAAGC ATGAGCTGGT GTCCTTCTGT TGTCCTGCGC 
TCTGAAAATG ATGAAATGAA TGAAAGAAGT TACAGTCTGG AAGGACTTGC ACCAGAGAAG 
GAGGGAAGGA AAAGTCTGGT TCAGCAGCGT GTCGGAGAGT CCAGCCAGGA GCCCAAATGT 
CCATCAAAGG TGGAAAAAGA AGACAGAGGC TCGCTTGTGT CTCTGACTGA AGAAGAGCAG 
GAGTCAGATC TAGGCGACTG CAGCAGTTTG GGCAGTCAGA AGTCCATCCA GTCGGGTCGG 
AGATGTGTCC CACATTCACA ACCCATCCTC ACCAAATCTG TGTCCATGGT GGCCATCAGT 
CAGAAGGACC TGGACGGATT GAGTTCATTC ACTGGAACAT CAGGATCATT AGAGTACAGT 
ATATCAGAGG AGGATCCTGG GCCATTACGG AGTGACAGTG AAGGCAAAAT TGGAGGAACC 
AAAGTGAGCA GGACATTCAG TTACCTCAAG AGCAAGATGA GCAAGAAGAA CAAGCAGGAG 
AAGGAGAAAG AGAAGAATAA GGAGCGTGAG CGAGATGCCA AAGACAAGGA GAGAAGAGCC 
TCAAACGGAC ATCTCTTCAC TACGGTCATG ACTGTACCCA CAATCCCCTG CCACCAGTGC 
AACAAACTCA TCAACACTAA AGACTCTATC CTCTGCACCA ATTGCAATGC ACAAGTCCAC 
AAAGGCTGCA AAGAGAGTCT TCCTGTCTGC GCTAAAGTCA AGATGAAGCA GCAAAAACAG 
CAGCAGACTG TTACAGATTC TGCCTCGACG CCTAGTGTCA CCCTCAGAAG TAAAACTCTT 
CCAGCAAGGG ATCGTCCGTG GTCCACAATA TCCATTCCCG ATGACCAGCC GGCTCCCCTC 
GCTCCTCCTC GGAAAAGCCC CAGCAGCATC ATGTCTTTCA ACAGCAACCC ACTGTCCAAG 
AGCATGTCCA TTAATAACAT AGCAGGACAA ATGGACGACA CTCCTCTTAA GAAGTTTCTG 
TCCCAGTCAA CGGACTCTCT TCACAAGACC AGTAAAGTCA CGGAGTCTAC AGAATCTCTT 
ACTGATGAGG GTACAGAGAT GATGGACAGC CAGCTGATGG GAGAATTTGA AGCTGACGCC 
AAAGAGCTGG AGGCTGATTC TTATAGCTTC ACGGTAGACA AGAAATTTCT TAAGCAGCTT 
AAAAAGGATG TTATCAAAAG GCAAGACGTC ATCTATGAGC TCATCCAGAC AGAAATGCAC 
CATGTAAGGA CCTTAAAGAT CATGGCTGAT GTTTACAGCA AAGGACTACT GAGAGAAGTT 
CAACTAGAAG CTCAGACGGT GGAGAAGATG TTCCCCATGT TGGACGATGT TCTGGATTTG 
CACACACAGT TCTTTTCACA CCTGCTTGAA AGAAAGAAGG AATCAGGGAG CCAAGAAGAG 
GGCGGCTTTG TCATCCAAAG GATTGGCGAT GTGCTGGTTT CTCAGTTCTC AGGTTCCAGT 
GCTGAGAGAA TGAAGAAGGT CTATGGGAAG TTCTGCGGCA GGCACAATGA AGCTGTGAAC 
TTCTACAAGG AGCTTCACAC CAAAGACAAA CGCTTTCAAG CTTTCATCAA GAAAAAGATG 
AGCAGCCCTG TTGTCCGACG GCTAAGCATT CCTGAGTGTA TATTGTTAGT CACACAACGA 
ATCACAAAAT ATCCCGTGTT ACTACAGAGA ATACTGCAGC ACACGAAAGA AAACGAGGAG 
GACCATGCAG ACGTTAGTCA AGGTCTGAAG CTGGTGAAGG AGGTGATTGC TGCCGTGGAC 
AATAAAGTGA ATGAGCATGA GAAGAAGAAG AGGCTAAAGG AAGTGTACAG CCGGACGGAC 
AACAAGTCCA TCATGAGGAT GAAGAGCGGG CAGATGTTTG CCAGAGAGGA TCTGTTACGT 
GGGCGGAAGT TAATTCACGA CGGGCCGCTG CAGCTCAAGA ACTCAGCTGG CAGACTCAAG 
GATGTCCAGG CTCTGCTGCT GAGGGACGTG ATTGTGTTCC TGCAGGAGAA AGATCAGAAG 
TACATTTTCG CCTCTTTGGA CCAGCGCTCC ACCGTCATCT CCCTGCAGAA GCTCATTGTG 
AGGGAAGTAG CCAATGAGGA GAAAGCTCTG TTTCTGATCA CAGCAGGCAT CGAGAAGCCC 
GAGATGGTAG AAGTGTACAC AAGCTCCAAA GAGGAGAGAA ACACATGGAT GCAGCTCATT 
CAGGAGGCCA TGCAGTCTAT ACTGTCCATT TCCTCCAGGG AGAGAGATGA TGATGAAGGT 
GTGCCAAGTG AAAACGAAGA GGACAGGAGA CTTCAGGAAA TCAAGGTCAA GGAGTTCAGA 
GATCAGCTGC ACAAAAGAGA CGAGCAGATC TTGACGTTAC TGGAGGAGAA GGTGAAGTTG 
TTCAGGGACA TGTGTGACTG TGGTGCACCG GACGAGACGG GGCTTCGTAA TCGAATGCTC 
TTCAGGGCCA CGACGGATGA TGTCACCAGA GGAGAGCCAA TCATGAAGGA GGCTCTGAAG 
GAAGTTGAAA CGCTGCAGGT TCTGGTTAAT GACAGTCTTG GCGGTGCGGT TCAGGAAGGT 
GTGTGCGCGG CTGGACATGT GACTTTGCCA CGACGAGCGG AAACATTTGG AGGTTTCGAC 
AGTCACCAGA TGAACATCTC TAAGAGCAAA GATGGAGACA GAGAGGAGCT GGAGGAGTCT 
GCAGAGCTCC GCAGGACTGA GTCAGACAGT GTGTTGAAGA AGACGAGTAA GACCAACCCT 
TTACTGCTGG TCAGGAGAAA CAATGAGCAG GTTCTTCAGA GCGTTGCTCA TCTTCATGAC 
CTGCTGAACA CATTACAAGC TGTGGTGGTC CAGCAGGACA CCTTCATCGA AGACCAGCGT 
CACGCTCATC CCTCCTCACG CCACCCATCC ATCTCCTCCT CTACCTCCTC TTTATCATCC 
ACCTCATCCC GGCCGAGCTC TCTAATCGAG CAGGAGAAAC AGCGCAGCCT GGAGAAGCAG 
CGGCAGGAGG TGGCCAGCCT CCAAAAACAG CAGGCGGCCC ACGCCGAGGA GAGGCGGCGC 
AGGGAAAAGC AGTGGGAGAC CCGAGAGAAA GAGCTAACCC AGAGGGAGAT GCAGATCCAG 
AGCCAAGAAG AGGAGGCGCA GAGGAGAGCC AGACAGCTGG AGGAGGAGAA ACAGGAGCTG 
CAGAGTAAGA AAGAGGAGTA TCAGAGAGAC CTGGCACGAC TGCGGGACAG CCAGAAGAGA 
CTGGAGAGAG AGAGAGAGCA GGTGCAGAGA GAGGCAGATG AGCTTAAACT GACTGAGGAA 
GTGACAAAGA ACCGGTCTCC GTCAACCACG TCTGAAGACT CCACGAAGTT CCAGAGCACC 
GGTTCATTAG ACCGTGACCC ATCAGAGATG GAGCTCTCCT CCTCCCCGTC AGCCAGAGAC 
TTCCTGTCCC GCATGGACTC CAAACGCAAA GGGAAAAACC TCAATCCATT CTCATCCAAC 
TCAAGCCAGA AGAGCCAGAA CAGCGAGGCT CAGAGCCAGA TGCCCAGCCG TCTGCTCCAG 
CTGGCCAAAC CCAAGGAGAA GAAGGACAAG AAGAAGAAGA AGGGGAAAGG CCAGCAGAGC 
CAGACTGCAG ACTCTCAGAG CACTGTTGTT CCAGGCGCTA CCCCAGACGG AGAGATCTTT 
TTCTGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

wu:fi15d04 ( XM_009297846.2 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_009296121.1 Danio rerio wu:fi15d04 (wu:fi15d04), transcript variant X11, mRNA.

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CloneID	ODa39940
Clone ID Related Accession (Same CDS sequence)	XM_009297846.2
Accession Version	XM_009297846.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7563bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-23
Organism	Danio rerio(Zebrafish)
Product	A-kinase anchor protein 13 isoform X11
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007136.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_009297846.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAGGGTG TTCCACAAGT GGCCAGTGCC ATGAAACTGA ATCCACAGCA AGCCCCATTA 
TATGGAGAGT GTCTGCTGAC GGTCCAGCTC TGTGAGGATG AGTGTGTTGA GGATGAGGAT 
GATGTGGAGT TTTACCTGCT CTTTGCTGGA TCCACACAGA GACATGTGTC CAGCACACTG 
AAGATCAGCC ATGTCACGCT GCAGGCCGTC TGTCCAGCTC ATGATCGCAG TGAAACGGTA 
CGGGTCACAC TATGCCAGGC TCGTCCCGGC GGCTCAGTGG ACCCAGTGTC CGAGGAGTGT 
TTCCAGTTTG TCCAGGACCT GGCTCTGGAC ATGGCCCAGT TCCTGGTAAA TGCGGCGGGG 
AAAACAGAAG GAGCGCTGCT GCTGGACGAG TGTCAGATCC CACTGAAAGA GTGCGAGCGG 
CTGGACGATA CTCTTGCTCT GGCTCTCCGG CATCTGCCTT TGCCTGCTGG ATGGAGCGTT 
TTGGGCACAG ATTTGGACAC GGGCACTTGC ACAGGCTCGG GCATCGCAGA GCCTGGACCA 
CACGATACAC TTTTGCACTT CGCTGCGAAG CGCGGACTAC GCAAGGTTGC ACTCTTTCTG 
CTCCAGCAAC CAGGGGGAAG AGAGGCTCTC CGGCTTCCCA ACAAACAGGG TCGCACACCG 
GCCAAGGTCG CCCAGAAGAG GGGACACTCT CAGCTCCACA AACTGCTCAC GGAGGTTGAC 
AATCTGCCCG AGCTGGAGAC GAAAACATCT AAGTGGTGTT ACCCAGAAGG CAGAGTCCTG 
CAACACCACC CCAAGCTAAA CACTTACACC CTTACCCTGG AGGATGTGCC CAGGAGCTCC 
CCACCAAGCC TTCAGTGTGA TGTGGAGGAG CTCCAGCGTC TCATTCAGTC ACATCAGAAG 
GAAGATCCTC CAACACCGCA TAAGTCAGCA CTGCTGATTC TCAATAGAGA CTCCAGTAAT 
AGCACAGAGA CAGATGACTG CCTTCCGCCC AACTCAGTGG AACCTGAGCA TCAGGAGCAC 
AACAAACAAG AAGAAACTTG CAAATCGCTT TTAAACAGCC TTACCTTTGA GGATGGATTG 
TGTGTTCATC AAAACGGCAC AGGAGATCAT GTCCAGTTGC TGGATGGGGA CGTGGAGCCT 
GACCTGCCAG CTGTAGGAGA TACAGGGAAT GCATTAGATA AGACTGACTG TTTGCAGCGG 
CGGGTAGATG GTGATTCTGT GGCTTTCAGT GCCCCTTCCT GTGGAAACCA GCGGGAGGAA 
GCCGATATAG AGCTCTGCAT AATCAGTGCC AAGCAGGGGG TTGCTTCACA GCCTGATAAA 
GGGAAGCAAG AAGAGCAAGA GGCTTGCCAG AAGAGGTCAG AGACTGAAAA GGACTATAAC 
ACTGAATCTG GCAACCTTGG GCTTCAAGAC ATGGGCCACG CACAATCTCA GGGTCTCCTC 
CCCACGGAAA CTGGTCAGCC TACACAAAGG GAGGTGGAGA GTGAGGGGAG TACAGATGTC 
TGTTGGCGTG GGGACTTACC AGAGGAAAGG GAAGGGGACA TGAGTAGCAA CCAGGAAATA 
GACTCATATT CTTCAGCACT TTCTTCCCAT AATCTAGAGG GAAACAGCCA GCACGATGGT 
GAGACTGAGC CCCAGTTGCC TACACAATGC TCAGAGGGAA CAGATGAAGC TAAATTGACT 
TTAATTCAGG TAAATTCTGA AGTTACTTTG TCTATACGAA GCGAGGATAC CGTTATTTCT 
GACAGTGTTG ACAAGCCACA TGGTCTCTTA GAGACGGAGA CAGGAAATCT CAGCGAACAC 
ACCAAAGGCA ACACTGAGTC AGCTGCTGAT TTGGTTGCGG ATGACGCTAG TGTAAAGCAG 
AACTCTGGAT TTAGCGTTTT AGAGGGGGAT CTTTTGGAAT CATTAAGTCT ACGGCCAAAC 
ATGGCACCGG GACCAGTGCA TAAGGTTCAG CAAGGTCTGT CACCAGTTCC TGAGACTTCC 
TCTCATGTAG GTCCAGCTTT GGACCCCTTT TCTGAGACTA CAGAAAGTTT TCAACAGCCG 
AATCAATCCA CACAGCTTTT AGAAACTGCA TTAGAATGTG AGGATGTTTC ATTAAATAAC 
TCAACCGGTG ACATTAATGC AATAGCAGGG GTTTCTGTTG ACATTTCTGC AGAGGTTTCA 
CTTTGCCAGA ATGAAGGGTG TCCTCCTGAT GGAAGCAAAG AAGAGGTAGC CCCCAGTGAG 
AGGATACCAG ACCTTAAAGA GCTTACGGAA GAGCCGGAAT CAGTTGAGGA CATTATTGTC 
TCAGATGTCT CAGATTTGGA TGACAAATCT CAACCTTTTG AAGAGTCTTC TTTGCCTATC 
GACTCTGAAT GTTTTATAAG AGGTGATTTC CAAAACGATG AGTCTACAGT ACAGTCTTCT 
GAATGTACTG GAGGTGACAT AAATGTAGGA ACACTCTTAG GAGAAGAAGA GGCAACTGAA 
AAAATAGAAC AGCCAGACTC TTCACTTAAA CTGGTTGAGC TAATTCCTGA GGAATATACT 
GGAGATGCCA CAAACGAAAA TAAAAAAATT GAGCCAGAGG AACAATCTAT TTTACTGCCA 
ACTTTGCAAG GGGAATCAGG ATTTAAGGAT GTTGCCACAG AGGAACCTGT ACAGGAAAGG 
GAAATTGACT CCACTTCAGA GGAAACAATC AGCGATCTCC CTGATCCATC GGAAATCACA 
AACAATGATT GTGCTGAATC CAATGCACAC TGCCAGCTCT CAGAATCCTC CAAGGAGAGC 
GCAAACACAT CCTCAGAACC TGCTGAATGT GTGAATGACC TAGACTTGTC TTGTGCTTTA 
CCTCAAACAG AAATCGAAGG CAGCAGACCT TCTGAGGGCC CCTTAATTGA TGGAGTAACA 
ATATGTGCTG AAATTCCCAT TGATGACAAA GGTGTTGACA TGGCTACCAA TATGAGTAAG 
ACGACCATCT CTGAGGATTG TCTGTCCTCT CCTATGGACA CCACCAGCTG TTCAGTTCTG 
GAGAGCTCCT CCATGTCTGA CCCACTCAAT GCCTCATCCT CAACAGCTGA GCTCACTTCC 
CCTTTGGATA TTAATAGTGG ATTGCATCAG GATTTGGAAC ACACTTCCCC AGTGGATATA 
GACAATGGTT TGCCTGAGGA ACCGCCGCCG TCAGACACAC CTGATTGCCT GAATGGATTA 
CATCAAGATT CTCCTGATAG TTTGATGGAG CACAGTGGAA CTCCTCAGCC ATGTTCTGAG 
GAACAGTGTG TGGATCAAAT CACAGATGAC AGCACTTCAG CAGAGGATGC AGCGGACGGA 
CACCAAGACT CCTCAGTAGA CATAGCAAAT TCCGAAGCGA TCCACAGAGA GACCGAAACA 
TTTTATTCAG TTGATGCCAG TCAAGACTCT GTACTCCAGG AGAAGAAATC TTCTTCAGTG 
AGCTCAGAAA TCCCCTCTTT GACCCCCTCA CCTGAGAGCA TACGTCACAA AGAGTCGCCA 
AGCACTATGC GAGGGTCCGC CAGCGATGGA GACTCTCTGT TTAGTCAGGA TCTGGGCGAG 
GACAGCGTTT TTAATAATAA GGCTGAGGAC AGCATTACCA TCACCAGTGG CGTGTCCATG 
TCCTATTCGT CCTCCACAGA TGACTCTTCC AGCTTGGGAA CCACTTGCGC CACCCCTCAG 
ACTGGCACAG AGGCTCCTGC ATCAGACCCC TTTCAGGAAC AGCCCACATC TACTGGAGCT 
TCTGGGGAAA CAGAGGAGGA AGAGAAAAAG GACCGTCTCA CTGAGGTTCC GGAGCGATCG 
GCCATTTTAC GGACCACCAT ACGATCACTG TCCCCTTTTC GAAGACACAG TTGGGGGCCG 
GGCAAAAATT TGGCAGGGGA GACAGAGATG AGTCAGCGCA GTTACAGTCT GGAAGGACTT 
GCACCAGAGA AGGAGGGAAG GAAAAGTCTG GTTCAGCAGC GTGTCGGAGA GTCCAGCCAG 
GAGCCCAAAT GTCCATCAAA GGTGGAAAAA GAAGACAGAG GCTCGCTTGT GTCTCTGACT 
GAAGAAGAGC AGGAGTCAGA TCTAGGCGAC TGCAGCAGTT TGGGCAGTCA GAAGTCCATC 
CAGTCGGGTC GGAGATGTGT CCCACATTCA CAACCCATCC TCACCAAATC TGTGTCCATG 
GTGGCCATCA GTCAGAAGGA CCTGGACGCA ATGGGACGGC TGCGACCCAA GCGACGAATC 
TCCTTCTCCT TCAGCATCTC GCCGATGCTC CCCAAATCTA AAACTCTCTT TTCTCTTGGC 
TCTTCATCCA GTGATGAGGA GGAGGCAGTC AGATTGAGTT CATTCACTGG AACATCAGGA 
TCATTAGAGT ACAGTATATC AGAGGAGGAT CCTGGGCCAT TACGGAGTGA CAGTGAAGGC 
AAAATTGGAG GAACCAAAGT GAGCAGGACA TTCAGTTACC TCAAGAGCAA GATGAGCAAG 
AAGAACAAGG AGAAGGAGAA AGAGAAGAAT AAGGAGCGTG AGCGAGATGC CAAAGACAAG 
GAGAGAAGAG CCTCAAACGG ACATCTCTTC ACTACGGTCA TGACTGTACC CACAATCCCC 
TGCCACCAGT GCAACAAACT CATCAACACT AAAGACTCTA TCCTCTGCAC CAATTGCAAT 
GCACAAGTCC ACAAAGGCTG CAAAGAGAGT CTTCCTGTCT GCGCTAAAGT CAAGATGAAG 
CAGCAAAAAC AGCAGCAGAC TGTTACAGAT TCTGCCTCGA CGCCTAGTGT CACCCTCAGA 
AGTAAAACTC TTCCAGCAAG GGATCGTCCG TGGTCCACAA TATCCATTCC CGATGACCAG 
CCGGCTCCCC TCGCTCCTCC TCGGAAAAGC CCCAGCAGCA TCATGTCTTT CAACAGCAAC 
CCACTGTCCA AGAGCATGTC CATTAATAAC ATAGCAGGAC AAATGGACGA CACTCCTCTT 
AAGAAGTTTC TGTCCCAGTC AACGGACTCT CTTCACAAGA CCAGTAAAGT CACGGAGTCT 
ACAGAATCTC TTACTGATGA GGGTACAGAG ATGATGGACA GCCAGCTGAT GGGAGAATTT 
GAAGCTGACG CCAAAGAGCT GGAGGCTGAT TCTTATAGCT TCACGGTAGA CAAGAAATTT 
CTTAAGCAGC TTAAAAAGGA TGTTATCAAA AGGCAAGACG TCATCTATGA GCTCATCCAG 
ACAGAAATGC ACCATGTAAG GACCTTAAAG ATCATGGCTG ATGTTTACAG CAAAGGACTA 
CTGAGAGAAG TTCAACTAGA AGCTCAGACG GTGGAGAAGA TGTTCCCCAT GTTGGACGAT 
GTTCTGGATT TGCACACACA GTTCTTTTCA CACCTGCTTG AAAGAAAGAA GGAATCAGGG 
AGCCAAGAAG AGGGCGGCTT TGTCATCCAA AGGATTGGCG ATGTGCTGGT TTCTCAGTTC 
TCAGGTTCCA GTGCTGAGAG AATGAAGAAG GTCTATGGGA AGTTCTGCGG CAGGCACAAT 
GAAGCTGTGA ACTTCTACAA GGAGCTTCAC ACCAAAGACA AACGCTTTCA AGCTTTCATC 
AAGAAAAAGA TGAGCAGCCC TGTTGTCCGA CGGCTAAGCA TTCCTGAGTG TATATTGTTA 
GTCACACAAC GAATCACAAA ATATCCCGTG TTACTACAGA GAATACTGCA GCACACGAAA 
GAAAACGAGG AGGACCATGC AGACGTTAGT CAAGGTCTGA AGCTGGTGAA GGAGGTGATT 
GCTGCCGTGG ACAATAAAGT GAATGAGCAT GAGAAGAAGA AGAGGCTAAA GGAAGTGTAC 
AGCCGGACGG ACAACAAGTC CATCATGAGG ATGAAGAGCG GGCAGATGTT TGCCAGAGAG 
GATCTGTTAC GTGGGCGGAA GTTAATTCAC GACGGGCCGC TGCAGCTCAA GAACTCAGCT 
GGCAGACTCA AGGATGTCCA GGCTCTGCTG CTGAGGGACG TGATTGTGTT CCTGCAGGAG 
AAAGATCAGA AGTACATTTT CGCCTCTTTG GACCAGCGCT CCACCGTCAT CTCCCTGCAG 
AAGCTCATTG TGAGGGAAGT AGCCAATGAG GAGAAAGCTC TGTTTCTGAT CACAGCAGGC 
ATCGAGAAGC CCGAGATGGT AGAAGTGTAC ACAAGCTCCA AAGAGGAGAG AAACACATGG 
ATGCAGCTCA TTCAGGAGGC CATGCAGTCT ATGGAGAGAG ATGATGATGA AGGTGTGCCA 
AGTGAAAACG AAGAGGACAG GAGACTTCAG GAAATCAAGG TCAAGGAGTT CAGAGATCAG 
CTGCACAAAA GAGACGAGCA GATCTTGACG TTACTGGAGG AGAAGGTGAA GTTGTTCAGG 
GACATGTGTG ACTGTGGTGC ACCGGACGAG ACGGGGCTTC GTAATCGAAT GCTCTTCAGG 
GCCACGACGG ATGATGTCAC CAGAGGAGAG CCAATCATGA AGGAGGCTCT GAAGGAAGTT 
GAAACGCTGC AGGTTCTGGT TAATGACAGT CTTGGCGGTG CGGTTCAGGA AGGTGTGTGC 
GCGGCTGGAC ATGTGACTTT GCCACGACGA GCGGAAACAT TTGGAGGTTT CGACAGTCAC 
CAGATGAACA TCTCTAAGAG CAAAGATGGA GACAGAGAGG AGCTGGAGGA GTCTGCAGAG 
CTCCGCAGGA CTGAGTCAGA CAGTGTGTTG AAGAAGACGA GTAAGACCAA CCCTTTACTG 
CTGGTCAGGA GAAACAATGA GCAGGTTCTT CAGAGCGTTG CTCATCTTCA TGACCTGCTG 
AACACATTAC AAGCTGTGGT GGTCCAGCAG GACACCTTCA TCGAAGACCA GCGTCACGCT 
CATCCCTCCT CACGCCACCC ATCCATCTCC TCCTCTACCT CCTCTTTATC ATCCACCTCA 
TCCCGGCCGA GCTCTCTAAT CGAGCAGGAG AAACAGCGCA GCCTGGAGAA GCAGCGGCAG 
GAGGTGGCCA GCCTCCAAAA ACAGCAGGCG GCCCACGCCG AGGAGAGGCG GCGCAGGGAA 
AAGCAGTGGG AGACCCGAGA GAAAGAGCTA ACCCAGAGGG AGATGCAGAT CCAGAGCCAA 
GAAGAGGAGG CGCAGAGGAG AGCCAGACAG CTGGAGGAGG AGAAACAGGA GCTGCAGAGT 
AAGAAAGAGG AGTATCAGAG AGACCTGGCA CGACTGCGGG ACAGCCAGAA GAGACTGGAG 
AGAGAGAGAG AGCAGGTGCA GAGAGAGGCA GATGAGCTTA AACTGACTGA GGAAGTGACA 
AAGAACCGGT CTCCGTCAAC CACGTCTGAA GACTCCACGA AGTTCCAGAG CACCGGTTCA 
TTAGACCGTG ACCCATCAGA GATGGAGCTC TCCTCCTCCC CGTCAGCCAG AGACTTCCTG 
TCCCGCATGG ACTCCAAACG CAAAGGGAAA AACCTCAATC CATTCTCATC CAACTCAAGC 
CAGAAGAGCC AGAACAGCGA GGCTCAGAGC CAGATGCCCA GCCGTCTGCT CCAGCTGGCC 
AAACCCAAGG AGAAGAAGGA CAAGAAGAAG AAGAAGGGGA AAGGCCAGCA GAGCCAGACT 
GCAGACTCTC AGAGCACTGT TGTTCCAGGC GCTACCCCAG ACGGAGAGAT CTTTTTCTGC 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_009296121.1
CDS	28..7590
Translation	MKGVPQVASAMKLNPQQAPLYGECLLTVQLCEDECVEDEDDVEFYLLFAGSTQRHVSSTLKISHVTLQAVCPAHDRSETVRVTLCQARPGGSVDPVSEECFQFVQDLALDMAQFLVNAAGKTEGALLLDECQIPLKECERLDDTLALALRHLPLPAGWSVLGTDLDTGTCTGSGIAEPGPHDTLLHFAAKRGLRKVALFLLQQPGGREALRLPNKQGRTPAKVAQKRGHSQLHKLLTEVDNLPELETKTSKWCYPEGRVLQHHPKLNTYTLTLEDVPRSSPPSLQCDVEELQRLIQSHQKEDPPTPHKSALLILNRDSSNSTETDDCLPPNSVEPEHQEHNKQEETCKSLLNSLTFEDGLCVHQNGTGDHVQLLDGDVEPDLPAVGDTGNALDKTDCLQRRVDGDSVAFSAPSCGNQREEADIELCIISAKQGVASQPDKGKQEEQEACQKRSETEKDYNTESGNLGLQDMGHAQSQGLLPTETGQPTQREVESEGSTDVCWRGDLPEEREGDMSSNQEIDSYSSALSSHNLEGNSQHDGETEPQLPTQCSEGTDEAKLTLIQVNSEVTLSIRSEDTVISDSVDKPHGLLETETGNLSEHTKGNTESAADLVADDASVKQNSGFSVLEGDLLESLSLRPNMAPGPVHKVQQGLSPVPETSSHVGPALDPFSETTESFQQPNQSTQLLETALECEDVSLNNSTGDINAIAGVSVDISAEVSLCQNEGCPPDGSKEEVAPSERIPDLKELTEEPESVEDIIVSDVSDLDDKSQPFEESSLPIDSECFIRGDFQNDESTVQSSECTGGDINVGTLLGEEEATEKIEQPDSSLKLVELIPEEYTGDATNENKKIEPEEQSILLPTLQGESGFKDVATEEPVQEREIDSTSEETISDLPDPSEITNNDCAESNAHCQLSESSKESANTSSEPAECVNDLDLSCALPQTEIEGSRPSEGPLIDGVTICAEIPIDDKGVDMATNMSKTTISEDCLSSPMDTTSCSVLESSSMSDPLNASSSTAELTSPLDINSGLHQDLEHTSPVDIDNGLPEEPPPSDTPDCLNGLHQDSPDSLMEHSGTPQPCSEEQCVDQITDDSTSAEDAADGHQDSSVDIANSEAIHRETETFYSVDASQDSVLQEKKSSSVSSEIPSLTPSPESIRHKESPSTMRGSASDGDSLFSQDLGEDSVFNNKAEDSITITSGVSMSYSSSTDDSSSLGTTCATPQTGTEAPASDPFQEQPTSTGASGETEEEEKKDRLTEVPERSAILRTTIRSLSPFRRHSWGPGKNLAGETEMSQRSYSLEGLAPEKEGRKSLVQQRVGESSQEPKCPSKVEKEDRGSLVSLTEEEQESDLGDCSSLGSQKSIQSGRRCVPHSQPILTKSVSMVAISQKDLDAMGRLRPKRRISFSFSISPMLPKSKTLFSLGSSSSDEEEAVRLSSFTGTSGSLEYSISEEDPGPLRSDSEGKIGGTKVSRTFSYLKSKMSKKNKEKEKEKNKERERDAKDKERRASNGHLFTTVMTVPTIPCHQCNKLINTKDSILCTNCNAQVHKGCKESLPVCAKVKMKQQKQQQTVTDSASTPSVTLRSKTLPARDRPWSTISIPDDQPAPLAPPRKSPSSIMSFNSNPLSKSMSINNIAGQMDDTPLKKFLSQSTDSLHKTSKVTESTESLTDEGTEMMDSQLMGEFEADAKELEADSYSFTVDKKFLKQLKKDVIKRQDVIYELIQTEMHHVRTLKIMADVYSKGLLREVQLEAQTVEKMFPMLDDVLDLHTQFFSHLLERKKESGSQEEGGFVIQRIGDVLVSQFSGSSAERMKKVYGKFCGRHNEAVNFYKELHTKDKRFQAFIKKKMSSPVVRRLSIPECILLVTQRITKYPVLLQRILQHTKENEEDHADVSQGLKLVKEVIAAVDNKVNEHEKKKRLKEVYSRTDNKSIMRMKSGQMFAREDLLRGRKLIHDGPLQLKNSAGRLKDVQALLLRDVIVFLQEKDQKYIFASLDQRSTVISLQKLIVREVANEEKALFLITAGIEKPEMVEVYTSSKEERNTWMQLIQEAMQSMERDDDEGVPSENEEDRRLQEIKVKEFRDQLHKRDEQILTLLEEKVKLFRDMCDCGAPDETGLRNRMLFRATTDDVTRGEPIMKEALKEVETLQVLVNDSLGGAVQEGVCAAGHVTLPRRAETFGGFDSHQMNISKSKDGDREELEESAELRRTESDSVLKKTSKTNPLLLVRRNNEQVLQSVAHLHDLLNTLQAVVVQQDTFIEDQRHAHPSSRHPSISSSTSSLSSTSSRPSSLIEQEKQRSLEKQRQEVASLQKQQAAHAEERRRREKQWETREKELTQREMQIQSQEEEAQRRARQLEEEKQELQSKKEEYQRDLARLRDSQKRLEREREQVQREADELKLTEEVTKNRSPSTTSEDSTKFQSTGSLDRDPSEMELSSSPSARDFLSRMDSKRKGKNLNPFSSNSSQKSQNSEAQSQMPSRLLQLAKPKEKKDKKKKKGKGQQSQTADSQSTVVPGATPDGEIFFC

Target ORF information:

RefSeq Version	XM_009297846.2
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio wu:fi15d04 (wu:fi15d04), transcript variant X11, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_009297846.2

ORF Insert Sequence:

ATGAAGGGTG TTCCACAAGT GGCCAGTGCC ATGAAACTGA ATCCACAGCA AGCCCCATTA 
TATGGAGAGT GTCTGCTGAC GGTCCAGCTC TGTGAGGATG AGTGTGTTGA GGATGAGGAT 
GATGTGGAGT TTTACCTGCT CTTTGCTGGA TCCACACAGA GACATGTGTC CAGCACACTG 
AAGATCAGCC ATGTCACGCT GCAGGCCGTC TGTCCAGCTC ATGATCGCAG TGAAACGGTA 
CGGGTCACAC TATGCCAGGC TCGTCCCGGC GGCTCAGTGG ACCCAGTGTC CGAGGAGTGT 
TTCCAGTTTG TCCAGGACCT GGCTCTGGAC ATGGCCCAGT TCCTGGTAAA TGCGGCGGGG 
AAAACAGAAG GAGCGCTGCT GCTGGACGAG TGTCAGATCC CACTGAAAGA GTGCGAGCGG 
CTGGACGATA CTCTTGCTCT GGCTCTCCGG CATCTGCCTT TGCCTGCTGG ATGGAGCGTT 
TTGGGCACAG ATTTGGACAC GGGCACTTGC ACAGGCTCGG GCATCGCAGA GCCTGGACCA 
CACGATACAC TTTTGCACTT CGCTGCGAAG CGCGGACTAC GCAAGGTTGC ACTCTTTCTG 
CTCCAGCAAC CAGGGGGAAG AGAGGCTCTC CGGCTTCCCA ACAAACAGGG TCGCACACCG 
GCCAAGGTCG CCCAGAAGAG GGGACACTCT CAGCTCCACA AACTGCTCAC GGAGGTTGAC 
AATCTGCCCG AGCTGGAGAC GAAAACATCT AAGTGGTGTT ACCCAGAAGG CAGAGTCCTG 
CAACACCACC CCAAGCTAAA CACTTACACC CTTACCCTGG AGGATGTGCC CAGGAGCTCC 
CCACCAAGCC TTCAGTGTGA TGTGGAGGAG CTCCAGCGTC TCATTCAGTC ACATCAGAAG 
GAAGATCCTC CAACACCGCA TAAGTCAGCA CTGCTGATTC TCAATAGAGA CTCCAGTAAT 
AGCACAGAGA CAGATGACTG CCTTCCGCCC AACTCAGTGG AACCTGAGCA TCAGGAGCAC 
AACAAACAAG AAGAAACTTG CAAATCGCTT TTAAACAGCC TTACCTTTGA GGATGGATTG 
TGTGTTCATC AAAACGGCAC AGGAGATCAT GTCCAGTTGC TGGATGGGGA CGTGGAGCCT 
GACCTGCCAG CTGTAGGAGA TACAGGGAAT GCATTAGATA AGACTGACTG TTTGCAGCGG 
CGGGTAGATG GTGATTCTGT GGCTTTCAGT GCCCCTTCCT GTGGAAACCA GCGGGAGGAA 
GCCGATATAG AGCTCTGCAT AATCAGTGCC AAGCAGGGGG TTGCTTCACA GCCTGATAAA 
GGGAAGCAAG AAGAGCAAGA GGCTTGCCAG AAGAGGTCAG AGACTGAAAA GGACTATAAC 
ACTGAATCTG GCAACCTTGG GCTTCAAGAC ATGGGCCACG CACAATCTCA GGGTCTCCTC 
CCCACGGAAA CTGGTCAGCC TACACAAAGG GAGGTGGAGA GTGAGGGGAG TACAGATGTC 
TGTTGGCGTG GGGACTTACC AGAGGAAAGG GAAGGGGACA TGAGTAGCAA CCAGGAAATA 
GACTCATATT CTTCAGCACT TTCTTCCCAT AATCTAGAGG GAAACAGCCA GCACGATGGT 
GAGACTGAGC CCCAGTTGCC TACACAATGC TCAGAGGGAA CAGATGAAGC TAAATTGACT 
TTAATTCAGG TAAATTCTGA AGTTACTTTG TCTATACGAA GCGAGGATAC CGTTATTTCT 
GACAGTGTTG ACAAGCCACA TGGTCTCTTA GAGACGGAGA CAGGAAATCT CAGCGAACAC 
ACCAAAGGCA ACACTGAGTC AGCTGCTGAT TTGGTTGCGG ATGACGCTAG TGTAAAGCAG 
AACTCTGGAT TTAGCGTTTT AGAGGGGGAT CTTTTGGAAT CATTAAGTCT ACGGCCAAAC 
ATGGCACCGG GACCAGTGCA TAAGGTTCAG CAAGGTCTGT CACCAGTTCC TGAGACTTCC 
TCTCATGTAG GTCCAGCTTT GGACCCCTTT TCTGAGACTA CAGAAAGTTT TCAACAGCCG 
AATCAATCCA CACAGCTTTT AGAAACTGCA TTAGAATGTG AGGATGTTTC ATTAAATAAC 
TCAACCGGTG ACATTAATGC AATAGCAGGG GTTTCTGTTG ACATTTCTGC AGAGGTTTCA 
CTTTGCCAGA ATGAAGGGTG TCCTCCTGAT GGAAGCAAAG AAGAGGTAGC CCCCAGTGAG 
AGGATACCAG ACCTTAAAGA GCTTACGGAA GAGCCGGAAT CAGTTGAGGA CATTATTGTC 
TCAGATGTCT CAGATTTGGA TGACAAATCT CAACCTTTTG AAGAGTCTTC TTTGCCTATC 
GACTCTGAAT GTTTTATAAG AGGTGATTTC CAAAACGATG AGTCTACAGT ACAGTCTTCT 
GAATGTACTG GAGGTGACAT AAATGTAGGA ACACTCTTAG GAGAAGAAGA GGCAACTGAA 
AAAATAGAAC AGCCAGACTC TTCACTTAAA CTGGTTGAGC TAATTCCTGA GGAATATACT 
GGAGATGCCA CAAACGAAAA TAAAAAAATT GAGCCAGAGG AACAATCTAT TTTACTGCCA 
ACTTTGCAAG GGGAATCAGG ATTTAAGGAT GTTGCCACAG AGGAACCTGT ACAGGAAAGG 
GAAATTGACT CCACTTCAGA GGAAACAATC AGCGATCTCC CTGATCCATC GGAAATCACA 
AACAATGATT GTGCTGAATC CAATGCACAC TGCCAGCTCT CAGAATCCTC CAAGGAGAGC 
GCAAACACAT CCTCAGAACC TGCTGAATGT GTGAATGACC TAGACTTGTC TTGTGCTTTA 
CCTCAAACAG AAATCGAAGG CAGCAGACCT TCTGAGGGCC CCTTAATTGA TGGAGTAACA 
ATATGTGCTG AAATTCCCAT TGATGACAAA GGTGTTGACA TGGCTACCAA TATGAGTAAG 
ACGACCATCT CTGAGGATTG TCTGTCCTCT CCTATGGACA CCACCAGCTG TTCAGTTCTG 
GAGAGCTCCT CCATGTCTGA CCCACTCAAT GCCTCATCCT CAACAGCTGA GCTCACTTCC 
CCTTTGGATA TTAATAGTGG ATTGCATCAG GATTTGGAAC ACACTTCCCC AGTGGATATA 
GACAATGGTT TGCCTGAGGA ACCGCCGCCG TCAGACACAC CTGATTGCCT GAATGGATTA 
CATCAAGATT CTCCTGATAG TTTGATGGAG CACAGTGGAA CTCCTCAGCC ATGTTCTGAG 
GAACAGTGTG TGGATCAAAT CACAGATGAC AGCACTTCAG CAGAGGATGC AGCGGACGGA 
CACCAAGACT CCTCAGTAGA CATAGCAAAT TCCGAAGCGA TCCACAGAGA GACCGAAACA 
TTTTATTCAG TTGATGCCAG TCAAGACTCT GTACTCCAGG AGAAGAAATC TTCTTCAGTG 
AGCTCAGAAA TCCCCTCTTT GACCCCCTCA CCTGAGAGCA TACGTCACAA AGAGTCGCCA 
AGCACTATGC GAGGGTCCGC CAGCGATGGA GACTCTCTGT TTAGTCAGGA TCTGGGCGAG 
GACAGCGTTT TTAATAATAA GGCTGAGGAC AGCATTACCA TCACCAGTGG CGTGTCCATG 
TCCTATTCGT CCTCCACAGA TGACTCTTCC AGCTTGGGAA CCACTTGCGC CACCCCTCAG 
ACTGGCACAG AGGCTCCTGC ATCAGACCCC TTTCAGGAAC AGCCCACATC TACTGGAGCT 
TCTGGGGAAA CAGAGGAGGA AGAGAAAAAG GACCGTCTCA CTGAGGTTCC GGAGCGATCG 
GCCATTTTAC GGACCACCAT ACGATCACTG TCCCCTTTTC GAAGACACAG TTGGGGGCCG 
GGCAAAAATT TGGCAGGGGA GACAGAGATG AGTCAGCGCA GTTACAGTCT GGAAGGACTT 
GCACCAGAGA AGGAGGGAAG GAAAAGTCTG GTTCAGCAGC GTGTCGGAGA GTCCAGCCAG 
GAGCCCAAAT GTCCATCAAA GGTGGAAAAA GAAGACAGAG GCTCGCTTGT GTCTCTGACT 
GAAGAAGAGC AGGAGTCAGA TCTAGGCGAC TGCAGCAGTT TGGGCAGTCA GAAGTCCATC 
CAGTCGGGTC GGAGATGTGT CCCACATTCA CAACCCATCC TCACCAAATC TGTGTCCATG 
GTGGCCATCA GTCAGAAGGA CCTGGACGCA ATGGGACGGC TGCGACCCAA GCGACGAATC 
TCCTTCTCCT TCAGCATCTC GCCGATGCTC CCCAAATCTA AAACTCTCTT TTCTCTTGGC 
TCTTCATCCA GTGATGAGGA GGAGGCAGTC AGATTGAGTT CATTCACTGG AACATCAGGA 
TCATTAGAGT ACAGTATATC AGAGGAGGAT CCTGGGCCAT TACGGAGTGA CAGTGAAGGC 
AAAATTGGAG GAACCAAAGT GAGCAGGACA TTCAGTTACC TCAAGAGCAA GATGAGCAAG 
AAGAACAAGG AGAAGGAGAA AGAGAAGAAT AAGGAGCGTG AGCGAGATGC CAAAGACAAG 
GAGAGAAGAG CCTCAAACGG ACATCTCTTC ACTACGGTCA TGACTGTACC CACAATCCCC 
TGCCACCAGT GCAACAAACT CATCAACACT AAAGACTCTA TCCTCTGCAC CAATTGCAAT 
GCACAAGTCC ACAAAGGCTG CAAAGAGAGT CTTCCTGTCT GCGCTAAAGT CAAGATGAAG 
CAGCAAAAAC AGCAGCAGAC TGTTACAGAT TCTGCCTCGA CGCCTAGTGT CACCCTCAGA 
AGTAAAACTC TTCCAGCAAG GGATCGTCCG TGGTCCACAA TATCCATTCC CGATGACCAG 
CCGGCTCCCC TCGCTCCTCC TCGGAAAAGC CCCAGCAGCA TCATGTCTTT CAACAGCAAC 
CCACTGTCCA AGAGCATGTC CATTAATAAC ATAGCAGGAC AAATGGACGA CACTCCTCTT 
AAGAAGTTTC TGTCCCAGTC AACGGACTCT CTTCACAAGA CCAGTAAAGT CACGGAGTCT 
ACAGAATCTC TTACTGATGA GGGTACAGAG ATGATGGACA GCCAGCTGAT GGGAGAATTT 
GAAGCTGACG CCAAAGAGCT GGAGGCTGAT TCTTATAGCT TCACGGTAGA CAAGAAATTT 
CTTAAGCAGC TTAAAAAGGA TGTTATCAAA AGGCAAGACG TCATCTATGA GCTCATCCAG 
ACAGAAATGC ACCATGTAAG GACCTTAAAG ATCATGGCTG ATGTTTACAG CAAAGGACTA 
CTGAGAGAAG TTCAACTAGA AGCTCAGACG GTGGAGAAGA TGTTCCCCAT GTTGGACGAT 
GTTCTGGATT TGCACACACA GTTCTTTTCA CACCTGCTTG AAAGAAAGAA GGAATCAGGG 
AGCCAAGAAG AGGGCGGCTT TGTCATCCAA AGGATTGGCG ATGTGCTGGT TTCTCAGTTC 
TCAGGTTCCA GTGCTGAGAG AATGAAGAAG GTCTATGGGA AGTTCTGCGG CAGGCACAAT 
GAAGCTGTGA ACTTCTACAA GGAGCTTCAC ACCAAAGACA AACGCTTTCA AGCTTTCATC 
AAGAAAAAGA TGAGCAGCCC TGTTGTCCGA CGGCTAAGCA TTCCTGAGTG TATATTGTTA 
GTCACACAAC GAATCACAAA ATATCCCGTG TTACTACAGA GAATACTGCA GCACACGAAA 
GAAAACGAGG AGGACCATGC AGACGTTAGT CAAGGTCTGA AGCTGGTGAA GGAGGTGATT 
GCTGCCGTGG ACAATAAAGT GAATGAGCAT GAGAAGAAGA AGAGGCTAAA GGAAGTGTAC 
AGCCGGACGG ACAACAAGTC CATCATGAGG ATGAAGAGCG GGCAGATGTT TGCCAGAGAG 
GATCTGTTAC GTGGGCGGAA GTTAATTCAC GACGGGCCGC TGCAGCTCAA GAACTCAGCT 
GGCAGACTCA AGGATGTCCA GGCTCTGCTG CTGAGGGACG TGATTGTGTT CCTGCAGGAG 
AAAGATCAGA AGTACATTTT CGCCTCTTTG GACCAGCGCT CCACCGTCAT CTCCCTGCAG 
AAGCTCATTG TGAGGGAAGT AGCCAATGAG GAGAAAGCTC TGTTTCTGAT CACAGCAGGC 
ATCGAGAAGC CCGAGATGGT AGAAGTGTAC ACAAGCTCCA AAGAGGAGAG AAACACATGG 
ATGCAGCTCA TTCAGGAGGC CATGCAGTCT ATGGAGAGAG ATGATGATGA AGGTGTGCCA 
AGTGAAAACG AAGAGGACAG GAGACTTCAG GAAATCAAGG TCAAGGAGTT CAGAGATCAG 
CTGCACAAAA GAGACGAGCA GATCTTGACG TTACTGGAGG AGAAGGTGAA GTTGTTCAGG 
GACATGTGTG ACTGTGGTGC ACCGGACGAG ACGGGGCTTC GTAATCGAAT GCTCTTCAGG 
GCCACGACGG ATGATGTCAC CAGAGGAGAG CCAATCATGA AGGAGGCTCT GAAGGAAGTT 
GAAACGCTGC AGGTTCTGGT TAATGACAGT CTTGGCGGTG CGGTTCAGGA AGGTGTGTGC 
GCGGCTGGAC ATGTGACTTT GCCACGACGA GCGGAAACAT TTGGAGGTTT CGACAGTCAC 
CAGATGAACA TCTCTAAGAG CAAAGATGGA GACAGAGAGG AGCTGGAGGA GTCTGCAGAG 
CTCCGCAGGA CTGAGTCAGA CAGTGTGTTG AAGAAGACGA GTAAGACCAA CCCTTTACTG 
CTGGTCAGGA GAAACAATGA GCAGGTTCTT CAGAGCGTTG CTCATCTTCA TGACCTGCTG 
AACACATTAC AAGCTGTGGT GGTCCAGCAG GACACCTTCA TCGAAGACCA GCGTCACGCT 
CATCCCTCCT CACGCCACCC ATCCATCTCC TCCTCTACCT CCTCTTTATC ATCCACCTCA 
TCCCGGCCGA GCTCTCTAAT CGAGCAGGAG AAACAGCGCA GCCTGGAGAA GCAGCGGCAG 
GAGGTGGCCA GCCTCCAAAA ACAGCAGGCG GCCCACGCCG AGGAGAGGCG GCGCAGGGAA 
AAGCAGTGGG AGACCCGAGA GAAAGAGCTA ACCCAGAGGG AGATGCAGAT CCAGAGCCAA 
GAAGAGGAGG CGCAGAGGAG AGCCAGACAG CTGGAGGAGG AGAAACAGGA GCTGCAGAGT 
AAGAAAGAGG AGTATCAGAG AGACCTGGCA CGACTGCGGG ACAGCCAGAA GAGACTGGAG 
AGAGAGAGAG AGCAGGTGCA GAGAGAGGCA GATGAGCTTA AACTGACTGA GGAAGTGACA 
AAGAACCGGT CTCCGTCAAC CACGTCTGAA GACTCCACGA AGTTCCAGAG CACCGGTTCA 
TTAGACCGTG ACCCATCAGA GATGGAGCTC TCCTCCTCCC CGTCAGCCAG AGACTTCCTG 
TCCCGCATGG ACTCCAAACG CAAAGGGAAA AACCTCAATC CATTCTCATC CAACTCAAGC 
CAGAAGAGCC AGAACAGCGA GGCTCAGAGC CAGATGCCCA GCCGTCTGCT CCAGCTGGCC 
AAACCCAAGG AGAAGAAGGA CAAGAAGAAG AAGAAGGGGA AAGGCCAGCA GAGCCAGACT 
GCAGACTCTC AGAGCACTGT TGTTCCAGGC GCTACCCCAG ACGGAGAGAT CTTTTTCTGC 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Gene Symbol	wu:fi15d04
Entrez Gene ID	327519
Full Name	wu:fi15d04
Synonyms	si:dkey-187e18.1,wu:fi40d09
General protein information	Preferred Names wu:fi15d04 Names A-kinase anchor protein 13 wu:fi15d04
Gene Type	protein-coding
Organism	Danio rerio(zebrafish)

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
30% OFF

wu:fi15d04 cDNA ORF clone, Danio rerio(zebrafish)

Gene

Clones

gRNAs

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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