LOC100844574 cDNA ORF clone, Brachypodium distachyon(stiff brome)

Gene
Clones
gRNAs

Summary

Gene Symbol	LOC100844574
Entrez Gene ID	100844574
Full Name	protein ROOT HAIR DEFECTIVE 3
Gene Type	protein-coding
Organism	Brachypodium distachyon(stiff brome)

mRNA and Protein(s)

mRNA	Protein	Name
XM_003569198.4	XP_003569246.1	protein ROOT HAIR DEFECTIVE 3
XM_003569198.3	XP_003569246.1	protein ROOT HAIR DEFECTIVE 3

Pathways from BioSystems
Gene Ontology

The following LOC100844574 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the LOC100844574 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OBr108504	XM_003569198.4 Latest version!	Brachypodium distachyon protein ROOT HAIR DEFECTIVE 3 (LOC100844574), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$426.30 $609.00
View Old Accession Versions >>
OBr108504	XM_003569198.3	Brachypodium distachyon protein ROOT HAIR DEFECTIVE 3 (LOC100844574), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$426.30 $609.00
Hide Old Accession Versions >>

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

LOC100844574 ( XM_003569198.4 ) cDNA ORF clone, Brachypodium distachyon(stiff brome) -> XP_003569246.1 Brachypodium distachyon protein ROOT HAIR DEFECTIVE 3 (LOC100844574), mRNA.

Price & Availability↑

CloneID	OBr108504
Clone ID Related Accession (Same CDS sequence)	XM_003569198.4 , XM_003569198.3
Accession Version	XM_003569198.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2421bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-03-26
Organism	Brachypodium distachyon(stiff brome)
Product	protein ROOT HAIR DEFECTIVE 3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_016132.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 27, 2018 this sequence version replaced XM_003569198.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Brachypodium distachyon Annotation Release 103 Annotation Version :: 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGGACG CCTGTCTTTC TACACAGCTT ATTGATGGAG ATGGTGTTTT CAATGTTTCC 
GGATTGGAAA ACTTTATGAA GGAAGTTAAA TTAGCCGACT GTGGGCTCTC TTATGCTGTT 
GTGTCAATAA TGGGCCCACA AAGTAGTGGA AAAAGTACAC TATTGAATCA TCTTTTCCGC 
ACCAATTTCA GGGAGATGGA TGCTTTTAGA GGAAGGTCAC AGACTACCAA AGGTATATGG 
ATGGCGAAGG CCCAGAATAT TGAACCATGC ACCCTTGTAA TGGATTTGGA AGGCACTGAT 
GGAAGAGAGA GGGGAGAGGA TGACACAGCC TTTGAGAAGC AAAGCGCTTT ATTTGCTTTG 
GCTGTTTCGG ATATTGTCTT GATCAATATG TGGTGCCACG ACATTGGTAG AGAACAGGCT 
GCGAACAAGC CTCTTTTGAA GACCGTTTTC CAGGTTATGA TGCGGTTGTT CAGTCCTCGC 
AAAACGACAA TGTTATTTGT TATTCGTGAC AAATCAAAGA CACCTCTGGA GAATCTTGAA 
CCTATTTTAA GGGAAGATAT TCAGAAGATA TGGGACGCCG TTCCTAAACC CCATGCTCAT 
AAAGAAACTC CACTAAGCGA ATTTTTCAAT GTACAAGTCG TGGCCCTTAA CAGTTATGAG 
GAAAAAGAAG AGTTATTTAA ACAGCAGGTT TCCGATTTAA GAGATAGATT TCAACATTCT 
ATTGCTCCTG GTGGGCTTGC TGGAGATCGT CGTGGTGTTG TTCCAGCATC AGGTTTTTCT 
TTTAGTTCGC AGCAATTCTG GAAGGTCATT AAGGAGAACA AAGACCTTGA CCTACCTGCT 
CATAAAGTTA TGGTGGCTAC CGTGCGTTGT GAGGAAATTG GTCATGAGAA AGTGACCAGT 
TTTACAGCCG ATGAGGAATG GCAACATTTT GAGGAGGCCG TTCAAAGTGA CTATGTACCA 
GGGTTTGGGA AAAAGATCAG CAGCCTTCTT GATAGATGTT TATCAGAATA TGACATGGAG 
GCCATTTATT TTGATGAAGG TGTCAGAACG TCAAAAAGAC AACAACTTGA GTCTAAACTC 
TTGCAGCTTG TCAATCCTGC ATACCAATCT CTCCTTGGTC ATTTACGGAC TAGAACTTTA 
GAAGCATTTA AGGAGTCCTT CGACAAGGCC CTAGAAAAGG AGGGATTTGC TGTTGCTGCT 
CGTAATTCTA TCCAGACTTT CTTGGAGAAG TTTGATAAAG GATCGGAAGA TGCTACTATC 
CAACAAGTGA AATGGGACCC CTCAAAAGTT AAGGATAAGC TTAAGCGTGA CATTGAGGCA 
CATGTGGTGT CAGTTCGTGC TACAAAACTT TCTGAACTTT GTGCCAGATA TGAGGGGAAG 
CTTACCAAGG CTCTGGCAGA TCCTGTCGAA GCCCTCTTAG ATACAGCCAG TGAAGACACC 
TGGCCAAATA TTAGAAAGCT TCTTCAACGC GAGACAAAAG CTGCTCTTTC AGGTCTTGAA 
TCTGCTATTT CAACTTTTGA GCTTGATGAA GCGACTGAGA AGGAACTCCT TTCAAGGCTG 
GAGAATCATG GAAGGAGCGT TGTTGAATCA AAGGCAAAAG AAGAAGCTGC AAGGGTCTTG 
ATCCGCATGA AGGACAGGTT CTCGACACTA TTCAGTCGTG ATGCTGACTC GATGCCAAGA 
GTATGGACAG GAAAGGAGGA CATTAAAGCA ATCACTAAAA CTGCTCGTTC AGCCTCCATG 
AAGTTGCTCT CTACAATGGC TGCAATTCGG TTGGATGAAG ATGTCGACAA CATAGATAAT 
ACTCTTTCCC TTGCTCTAGT TGACAGTGCA AGGCCTGGCA CTACAGATAG GAGTATCCAA 
TCGCTTGATC CACTCGCATC CAGTTCATGG GAAAGAATTC CAGAGGAGAG AACTTTGATC 
TCCCCTGTCC AGTGCAAATC TTTGTGGAGG CAATTTAAGG CGGAAACTGA ATATACCGTC 
ACTCAAGCCA TAGCTGCACA GGAAGCAAAT AAGAGGAACA ACAACTGGCT GCCTCCTCCA 
TGGGCACTTG CTGCAATGGT TATCCTTGGG TTTAACGAGT TTATGACATT ACTGAGGAAT 
CCATTGTACA TGGGTGTCAT ATTTGTTGCT TTCCTTGTTG TGAAAGCTGT CTGGGTACAA 
CTAGACATTG CCAATGAGTT CCGAAATGGA TTTCTTCCGG CCGTCCTTTC ACTGTCCACA 
AAGTTTGTTC CCACAATAAT GAACATTCTG AAGAGACTAG CTGATGAGGG ACACACACCT 
GCAGCCCCAG AGCAAAGAGA GATGGAACTC CAACCAAAAT CCACAAGGAA TAGTTCAAAT 
AGCAATGTGA CATCAGCAGG CTCCTCCAGC ATAACCTCTT CAGAGAATGG ACCTGAATAT 
TCAAGCCCGA TAGCGCAATG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_003569246.1
CDS	164..2584
Translation	MEDACLSTQLIDGDGVFNVSGLENFMKEVKLADCGLSYAVVSIMGPQSSGKSTLLNHLFRTNFREMDAFRGRSQTTKGIWMAKAQNIEPCTLVMDLEGTDGRERGEDDTAFEKQSALFALAVSDIVLINMWCHDIGREQAANKPLLKTVFQVMMRLFSPRKTTMLFVIRDKSKTPLENLEPILREDIQKIWDAVPKPHAHKETPLSEFFNVQVVALNSYEEKEELFKQQVSDLRDRFQHSIAPGGLAGDRRGVVPASGFSFSSQQFWKVIKENKDLDLPAHKVMVATVRCEEIGHEKVTSFTADEEWQHFEEAVQSDYVPGFGKKISSLLDRCLSEYDMEAIYFDEGVRTSKRQQLESKLLQLVNPAYQSLLGHLRTRTLEAFKESFDKALEKEGFAVAARNSIQTFLEKFDKGSEDATIQQVKWDPSKVKDKLKRDIEAHVVSVRATKLSELCARYEGKLTKALADPVEALLDTASEDTWPNIRKLLQRETKAALSGLESAISTFELDEATEKELLSRLENHGRSVVESKAKEEAARVLIRMKDRFSTLFSRDADSMPRVWTGKEDIKAITKTARSASMKLLSTMAAIRLDEDVDNIDNTLSLALVDSARPGTTDRSIQSLDPLASSSWERIPEERTLISPVQCKSLWRQFKAETEYTVTQAIAAQEANKRNNNWLPPPWALAAMVILGFNEFMTLLRNPLYMGVIFVAFLVVKAVWVQLDIANEFRNGFLPAVLSLSTKFVPTIMNILKRLADEGHTPAAPEQREMELQPKSTRNSSNSNVTSAGSSSITSSENGPEYSSPIAQ

Target ORF information:

RefSeq Version	XM_003569198.4
Organism	Brachypodium distachyon(stiff brome)
Definition	Brachypodium distachyon protein ROOT HAIR DEFECTIVE 3 (LOC100844574), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_003569198.4

ORF Insert Sequence:

ATGGAGGACG CCTGTCTTTC TACACAGCTT ATTGATGGAG ATGGTGTTTT CAATGTTTCC 
GGATTGGAAA ACTTTATGAA GGAAGTTAAA TTAGCCGACT GTGGGCTCTC TTATGCTGTT 
GTGTCAATAA TGGGCCCACA AAGTAGTGGA AAAAGTACAC TATTGAATCA TCTTTTCCGC 
ACCAATTTCA GGGAGATGGA TGCTTTTAGA GGAAGGTCAC AGACTACCAA AGGTATATGG 
ATGGCGAAGG CCCAGAATAT TGAACCATGC ACCCTTGTAA TGGATTTGGA AGGCACTGAT 
GGAAGAGAGA GGGGAGAGGA TGACACAGCC TTTGAGAAGC AAAGCGCTTT ATTTGCTTTG 
GCTGTTTCGG ATATTGTCTT GATCAATATG TGGTGCCACG ACATTGGTAG AGAACAGGCT 
GCGAACAAGC CTCTTTTGAA GACCGTTTTC CAGGTTATGA TGCGGTTGTT CAGTCCTCGC 
AAAACGACAA TGTTATTTGT TATTCGTGAC AAATCAAAGA CACCTCTGGA GAATCTTGAA 
CCTATTTTAA GGGAAGATAT TCAGAAGATA TGGGACGCCG TTCCTAAACC CCATGCTCAT 
AAAGAAACTC CACTAAGCGA ATTTTTCAAT GTACAAGTCG TGGCCCTTAA CAGTTATGAG 
GAAAAAGAAG AGTTATTTAA ACAGCAGGTT TCCGATTTAA GAGATAGATT TCAACATTCT 
ATTGCTCCTG GTGGGCTTGC TGGAGATCGT CGTGGTGTTG TTCCAGCATC AGGTTTTTCT 
TTTAGTTCGC AGCAATTCTG GAAGGTCATT AAGGAGAACA AAGACCTTGA CCTACCTGCT 
CATAAAGTTA TGGTGGCTAC CGTGCGTTGT GAGGAAATTG GTCATGAGAA AGTGACCAGT 
TTTACAGCCG ATGAGGAATG GCAACATTTT GAGGAGGCCG TTCAAAGTGA CTATGTACCA 
GGGTTTGGGA AAAAGATCAG CAGCCTTCTT GATAGATGTT TATCAGAATA TGACATGGAG 
GCCATTTATT TTGATGAAGG TGTCAGAACG TCAAAAAGAC AACAACTTGA GTCTAAACTC 
TTGCAGCTTG TCAATCCTGC ATACCAATCT CTCCTTGGTC ATTTACGGAC TAGAACTTTA 
GAAGCATTTA AGGAGTCCTT CGACAAGGCC CTAGAAAAGG AGGGATTTGC TGTTGCTGCT 
CGTAATTCTA TCCAGACTTT CTTGGAGAAG TTTGATAAAG GATCGGAAGA TGCTACTATC 
CAACAAGTGA AATGGGACCC CTCAAAAGTT AAGGATAAGC TTAAGCGTGA CATTGAGGCA 
CATGTGGTGT CAGTTCGTGC TACAAAACTT TCTGAACTTT GTGCCAGATA TGAGGGGAAG 
CTTACCAAGG CTCTGGCAGA TCCTGTCGAA GCCCTCTTAG ATACAGCCAG TGAAGACACC 
TGGCCAAATA TTAGAAAGCT TCTTCAACGC GAGACAAAAG CTGCTCTTTC AGGTCTTGAA 
TCTGCTATTT CAACTTTTGA GCTTGATGAA GCGACTGAGA AGGAACTCCT TTCAAGGCTG 
GAGAATCATG GAAGGAGCGT TGTTGAATCA AAGGCAAAAG AAGAAGCTGC AAGGGTCTTG 
ATCCGCATGA AGGACAGGTT CTCGACACTA TTCAGTCGTG ATGCTGACTC GATGCCAAGA 
GTATGGACAG GAAAGGAGGA CATTAAAGCA ATCACTAAAA CTGCTCGTTC AGCCTCCATG 
AAGTTGCTCT CTACAATGGC TGCAATTCGG TTGGATGAAG ATGTCGACAA CATAGATAAT 
ACTCTTTCCC TTGCTCTAGT TGACAGTGCA AGGCCTGGCA CTACAGATAG GAGTATCCAA 
TCGCTTGATC CACTCGCATC CAGTTCATGG GAAAGAATTC CAGAGGAGAG AACTTTGATC 
TCCCCTGTCC AGTGCAAATC TTTGTGGAGG CAATTTAAGG CGGAAACTGA ATATACCGTC 
ACTCAAGCCA TAGCTGCACA GGAAGCAAAT AAGAGGAACA ACAACTGGCT GCCTCCTCCA 
TGGGCACTTG CTGCAATGGT TATCCTTGGG TTTAACGAGT TTATGACATT ACTGAGGAAT 
CCATTGTACA TGGGTGTCAT ATTTGTTGCT TTCCTTGTTG TGAAAGCTGT CTGGGTACAA 
CTAGACATTG CCAATGAGTT CCGAAATGGA TTTCTTCCGG CCGTCCTTTC ACTGTCCACA 
AAGTTTGTTC CCACAATAAT GAACATTCTG AAGAGACTAG CTGATGAGGG ACACACACCT 
GCAGCCCCAG AGCAAAGAGA GATGGAACTC CAACCAAAAT CCACAAGGAA TAGTTCAAAT 
AGCAATGTGA CATCAGCAGG CTCCTCCAGC ATAACCTCTT CAGAGAATGG ACCTGAATAT 
TCAAGCCCGA TAGCGCAATG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC100844574 ( XM_003569198.3 ) cDNA ORF clone, Brachypodium distachyon(stiff brome) -> XP_003569246.1 Brachypodium distachyon protein ROOT HAIR DEFECTIVE 3 (LOC100844574), mRNA.

Price & Availability↑

CloneID	OBr108504
Clone ID Related Accession (Same CDS sequence)	XM_003569198.4 , XM_003569198.3
Accession Version	XM_003569198.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2421bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-12-07
Organism	Brachypodium distachyon(stiff brome)
Product	protein ROOT HAIR DEFECTIVE 3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014576699.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 8, 2015 this sequence version replaced XM_003569198.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Brachypodium distachyon Annotation Release 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGGACG CCTGTCTTTC TACACAGCTT ATTGATGGAG ATGGTGTTTT CAATGTTTCC 
GGATTGGAAA ACTTTATGAA GGAAGTTAAA TTAGCCGACT GTGGGCTCTC TTATGCTGTT 
GTGTCAATAA TGGGCCCACA AAGTAGTGGA AAAAGTACAC TATTGAATCA TCTTTTCCGC 
ACCAATTTCA GGGAGATGGA TGCTTTTAGA GGAAGGTCAC AGACTACCAA AGGTATATGG 
ATGGCGAAGG CCCAGAATAT TGAACCATGC ACCCTTGTAA TGGATTTGGA AGGCACTGAT 
GGAAGAGAGA GGGGAGAGGA TGACACAGCC TTTGAGAAGC AAAGCGCTTT ATTTGCTTTG 
GCTGTTTCGG ATATTGTCTT GATCAATATG TGGTGCCACG ACATTGGTAG AGAACAGGCT 
GCGAACAAGC CTCTTTTGAA GACCGTTTTC CAGGTTATGA TGCGGTTGTT CAGTCCTCGC 
AAAACGACAA TGTTATTTGT TATTCGTGAC AAATCAAAGA CACCTCTGGA GAATCTTGAA 
CCTATTTTAA GGGAAGATAT TCAGAAGATA TGGGACGCCG TTCCTAAACC CCATGCTCAT 
AAAGAAACTC CACTAAGCGA ATTTTTCAAT GTACAAGTCG TGGCCCTTAA CAGTTATGAG 
GAAAAAGAAG AGTTATTTAA ACAGCAGGTT TCCGATTTAA GAGATAGATT TCAACATTCT 
ATTGCTCCTG GTGGGCTTGC TGGAGATCGT CGTGGTGTTG TTCCAGCATC AGGTTTTTCT 
TTTAGTTCGC AGCAATTCTG GAAGGTCATT AAGGAGAACA AAGACCTTGA CCTACCTGCT 
CATAAAGTTA TGGTGGCTAC CGTGCGTTGT GAGGAAATTG GTCATGAGAA AGTGACCAGT 
TTTACAGCCG ATGAGGAATG GCAACATTTT GAGGAGGCCG TTCAAAGTGA CTATGTACCA 
GGGTTTGGGA AAAAGATCAG CAGCCTTCTT GATAGATGTT TATCAGAATA TGACATGGAG 
GCCATTTATT TTGATGAAGG TGTCAGAACG TCAAAAAGAC AACAACTTGA GTCTAAACTC 
TTGCAGCTTG TCAATCCTGC ATACCAATCT CTCCTTGGTC ATTTACGGAC TAGAACTTTA 
GAAGCATTTA AGGAGTCCTT CGACAAGGCC CTAGAAAAGG AGGGATTTGC TGTTGCTGCT 
CGTAATTCTA TCCAGACTTT CTTGGAGAAG TTTGATAAAG GATCGGAAGA TGCTACTATC 
CAACAAGTGA AATGGGACCC CTCAAAAGTT AAGGATAAGC TTAAGCGTGA CATTGAGGCA 
CATGTGGTGT CAGTTCGTGC TACAAAACTT TCTGAACTTT GTGCCAGATA TGAGGGGAAG 
CTTACCAAGG CTCTGGCAGA TCCTGTCGAA GCCCTCTTAG ATACAGCCAG TGAAGACACC 
TGGCCAAATA TTAGAAAGCT TCTTCAACGC GAGACAAAAG CTGCTCTTTC AGGTCTTGAA 
TCTGCTATTT CAACTTTTGA GCTTGATGAA GCGACTGAGA AGGAACTCCT TTCAAGGCTG 
GAGAATCATG GAAGGAGCGT TGTTGAATCA AAGGCAAAAG AAGAAGCTGC AAGGGTCTTG 
ATCCGCATGA AGGACAGGTT CTCGACACTA TTCAGTCGTG ATGCTGACTC GATGCCAAGA 
GTATGGACAG GAAAGGAGGA CATTAAAGCA ATCACTAAAA CTGCTCGTTC AGCCTCCATG 
AAGTTGCTCT CTACAATGGC TGCAATTCGG TTGGATGAAG ATGTCGACAA CATAGATAAT 
ACTCTTTCCC TTGCTCTAGT TGACAGTGCA AGGCCTGGCA CTACAGATAG GAGTATCCAA 
TCGCTTGATC CACTCGCATC CAGTTCATGG GAAAGAATTC CAGAGGAGAG AACTTTGATC 
TCCCCTGTCC AGTGCAAATC TTTGTGGAGG CAATTTAAGG CGGAAACTGA ATATACCGTC 
ACTCAAGCCA TAGCTGCACA GGAAGCAAAT AAGAGGAACA ACAACTGGCT GCCTCCTCCA 
TGGGCACTTG CTGCAATGGT TATCCTTGGG TTTAACGAGT TTATGACATT ACTGAGGAAT 
CCATTGTACA TGGGTGTCAT ATTTGTTGCT TTCCTTGTTG TGAAAGCTGT CTGGGTACAA 
CTAGACATTG CCAATGAGTT CCGAAATGGA TTTCTTCCGG CCGTCCTTTC ACTGTCCACA 
AAGTTTGTTC CCACAATAAT GAACATTCTG AAGAGACTAG CTGATGAGGG ACACACACCT 
GCAGCCCCAG AGCAAAGAGA GATGGAACTC CAACCAAAAT CCACAAGGAA TAGTTCAAAT 
AGCAATGTGA CATCAGCAGG CTCCTCCAGC ATAACCTCTT CAGAGAATGG ACCTGAATAT 
TCAAGCCCGA TAGCGCAATG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_003569246.1
CDS	161..2581
Translation	MEDACLSTQLIDGDGVFNVSGLENFMKEVKLADCGLSYAVVSIMGPQSSGKSTLLNHLFRTNFREMDAFRGRSQTTKGIWMAKAQNIEPCTLVMDLEGTDGRERGEDDTAFEKQSALFALAVSDIVLINMWCHDIGREQAANKPLLKTVFQVMMRLFSPRKTTMLFVIRDKSKTPLENLEPILREDIQKIWDAVPKPHAHKETPLSEFFNVQVVALNSYEEKEELFKQQVSDLRDRFQHSIAPGGLAGDRRGVVPASGFSFSSQQFWKVIKENKDLDLPAHKVMVATVRCEEIGHEKVTSFTADEEWQHFEEAVQSDYVPGFGKKISSLLDRCLSEYDMEAIYFDEGVRTSKRQQLESKLLQLVNPAYQSLLGHLRTRTLEAFKESFDKALEKEGFAVAARNSIQTFLEKFDKGSEDATIQQVKWDPSKVKDKLKRDIEAHVVSVRATKLSELCARYEGKLTKALADPVEALLDTASEDTWPNIRKLLQRETKAALSGLESAISTFELDEATEKELLSRLENHGRSVVESKAKEEAARVLIRMKDRFSTLFSRDADSMPRVWTGKEDIKAITKTARSASMKLLSTMAAIRLDEDVDNIDNTLSLALVDSARPGTTDRSIQSLDPLASSSWERIPEERTLISPVQCKSLWRQFKAETEYTVTQAIAAQEANKRNNNWLPPPWALAAMVILGFNEFMTLLRNPLYMGVIFVAFLVVKAVWVQLDIANEFRNGFLPAVLSLSTKFVPTIMNILKRLADEGHTPAAPEQREMELQPKSTRNSSNSNVTSAGSSSITSSENGPEYSSPIAQ

Target ORF information:

RefSeq Version	XM_003569198.3
Organism	Brachypodium distachyon(stiff brome)
Definition	Brachypodium distachyon protein ROOT HAIR DEFECTIVE 3 (LOC100844574), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_003569198.3

ORF Insert Sequence:

ATGGAGGACG CCTGTCTTTC TACACAGCTT ATTGATGGAG ATGGTGTTTT CAATGTTTCC 
GGATTGGAAA ACTTTATGAA GGAAGTTAAA TTAGCCGACT GTGGGCTCTC TTATGCTGTT 
GTGTCAATAA TGGGCCCACA AAGTAGTGGA AAAAGTACAC TATTGAATCA TCTTTTCCGC 
ACCAATTTCA GGGAGATGGA TGCTTTTAGA GGAAGGTCAC AGACTACCAA AGGTATATGG 
ATGGCGAAGG CCCAGAATAT TGAACCATGC ACCCTTGTAA TGGATTTGGA AGGCACTGAT 
GGAAGAGAGA GGGGAGAGGA TGACACAGCC TTTGAGAAGC AAAGCGCTTT ATTTGCTTTG 
GCTGTTTCGG ATATTGTCTT GATCAATATG TGGTGCCACG ACATTGGTAG AGAACAGGCT 
GCGAACAAGC CTCTTTTGAA GACCGTTTTC CAGGTTATGA TGCGGTTGTT CAGTCCTCGC 
AAAACGACAA TGTTATTTGT TATTCGTGAC AAATCAAAGA CACCTCTGGA GAATCTTGAA 
CCTATTTTAA GGGAAGATAT TCAGAAGATA TGGGACGCCG TTCCTAAACC CCATGCTCAT 
AAAGAAACTC CACTAAGCGA ATTTTTCAAT GTACAAGTCG TGGCCCTTAA CAGTTATGAG 
GAAAAAGAAG AGTTATTTAA ACAGCAGGTT TCCGATTTAA GAGATAGATT TCAACATTCT 
ATTGCTCCTG GTGGGCTTGC TGGAGATCGT CGTGGTGTTG TTCCAGCATC AGGTTTTTCT 
TTTAGTTCGC AGCAATTCTG GAAGGTCATT AAGGAGAACA AAGACCTTGA CCTACCTGCT 
CATAAAGTTA TGGTGGCTAC CGTGCGTTGT GAGGAAATTG GTCATGAGAA AGTGACCAGT 
TTTACAGCCG ATGAGGAATG GCAACATTTT GAGGAGGCCG TTCAAAGTGA CTATGTACCA 
GGGTTTGGGA AAAAGATCAG CAGCCTTCTT GATAGATGTT TATCAGAATA TGACATGGAG 
GCCATTTATT TTGATGAAGG TGTCAGAACG TCAAAAAGAC AACAACTTGA GTCTAAACTC 
TTGCAGCTTG TCAATCCTGC ATACCAATCT CTCCTTGGTC ATTTACGGAC TAGAACTTTA 
GAAGCATTTA AGGAGTCCTT CGACAAGGCC CTAGAAAAGG AGGGATTTGC TGTTGCTGCT 
CGTAATTCTA TCCAGACTTT CTTGGAGAAG TTTGATAAAG GATCGGAAGA TGCTACTATC 
CAACAAGTGA AATGGGACCC CTCAAAAGTT AAGGATAAGC TTAAGCGTGA CATTGAGGCA 
CATGTGGTGT CAGTTCGTGC TACAAAACTT TCTGAACTTT GTGCCAGATA TGAGGGGAAG 
CTTACCAAGG CTCTGGCAGA TCCTGTCGAA GCCCTCTTAG ATACAGCCAG TGAAGACACC 
TGGCCAAATA TTAGAAAGCT TCTTCAACGC GAGACAAAAG CTGCTCTTTC AGGTCTTGAA 
TCTGCTATTT CAACTTTTGA GCTTGATGAA GCGACTGAGA AGGAACTCCT TTCAAGGCTG 
GAGAATCATG GAAGGAGCGT TGTTGAATCA AAGGCAAAAG AAGAAGCTGC AAGGGTCTTG 
ATCCGCATGA AGGACAGGTT CTCGACACTA TTCAGTCGTG ATGCTGACTC GATGCCAAGA 
GTATGGACAG GAAAGGAGGA CATTAAAGCA ATCACTAAAA CTGCTCGTTC AGCCTCCATG 
AAGTTGCTCT CTACAATGGC TGCAATTCGG TTGGATGAAG ATGTCGACAA CATAGATAAT 
ACTCTTTCCC TTGCTCTAGT TGACAGTGCA AGGCCTGGCA CTACAGATAG GAGTATCCAA 
TCGCTTGATC CACTCGCATC CAGTTCATGG GAAAGAATTC CAGAGGAGAG AACTTTGATC 
TCCCCTGTCC AGTGCAAATC TTTGTGGAGG CAATTTAAGG CGGAAACTGA ATATACCGTC 
ACTCAAGCCA TAGCTGCACA GGAAGCAAAT AAGAGGAACA ACAACTGGCT GCCTCCTCCA 
TGGGCACTTG CTGCAATGGT TATCCTTGGG TTTAACGAGT TTATGACATT ACTGAGGAAT 
CCATTGTACA TGGGTGTCAT ATTTGTTGCT TTCCTTGTTG TGAAAGCTGT CTGGGTACAA 
CTAGACATTG CCAATGAGTT CCGAAATGGA TTTCTTCCGG CCGTCCTTTC ACTGTCCACA 
AAGTTTGTTC CCACAATAAT GAACATTCTG AAGAGACTAG CTGATGAGGG ACACACACCT 
GCAGCCCCAG AGCAAAGAGA GATGGAACTC CAACCAAAAT CCACAAGGAA TAGTTCAAAT 
AGCAATGTGA CATCAGCAGG CTCCTCCAGC ATAACCTCTT CAGAGAATGG ACCTGAATAT 
TCAAGCCCGA TAGCGCAATG A