LOC108992272 cDNA ORF clone, Juglans regia(English walnut)

Gene
Clones
gRNAs

Summary

Gene Symbol	LOC108992272
Entrez Gene ID	108992272
Full Name	probable leucine-rich repeat receptor-like protein kinase At5g63930
Gene Type	protein-coding
Organism	Juglans regia(English walnut)

mRNA and Protein(s)

mRNA	Protein	Name
XM_018966779.1	XP_018822324.1	receptor-like protein kinase isoform X1
XM_018966780.1	XP_018822325.1	probable leucine-rich repeat receptor-like protein kinase At5g63930 isoform X2

Pathways from BioSystems
Gene Ontology

The following LOC108992272 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the LOC108992272 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OJu35600	XM_018966779.1 Latest version!	Juglans regia probable leucine-rich repeat receptor-like protein kinase At5g63930 (LOC108992272), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$629.30 $899.00
OJu35601	XM_018966780.1 Latest version!	Juglans regia probable leucine-rich repeat receptor-like protein kinase At5g63930 (LOC108992272), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$629.30 $899.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

LOC108992272 ( XM_018966779.1 ) cDNA ORF clone, Juglans regia(English walnut) -> XP_018822324.1 Juglans regia probable leucine-rich repeat receptor-like protein kinase At5g63930 (LOC108992272), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OJu35600
Clone ID Related Accession (Same CDS sequence)	XM_018966779.1
Accession Version	XM_018966779.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3411bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-11-02
Organism	Juglans regia(English walnut)
Product	receptor-like protein kinase isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_017443529.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Juglans regia Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTTCTTGC TGTTTTCATA CTATGTTCTT TCCTTTATTG GGTTTCTGTT GGTTAACTCA 
GTTTCATTGC CTCCATCAGC CATATACCTT ATTGAATTCC GCCATAGCCT CCCCAAACAT 
TCTCAGCATC TCCTGCTATG GAACGAGTCC AATTCACCTT CCTCACCTTG CCAATGGCCA 
GGAGTTCATT GCTATCCCAA GAACAGCTTT CAAGTCAAGG TCTTAAACCT CTCAGGCTTT 
GGACTCTCTG GTGTCTTGAA CAACTCGATT TCCCATCTCT GCCACCATAA ACGTTTGCTC 
TCTCTTGACC TCAGTGGCAA CAGTTTTAGT GGTGAAATCC CCCTGCTGCT TGGAAACTGT 
GCTCAACTAA ATACCATTCT TCTCAACGAC AATGACTTCA GAGGGCCAAT CCCACCTGAG 
CTTTTCGGAT CAAAGCGGCT TTCACGGCTT GATTTGGGAT ATAATTCTCT CTCTGGTAAT 
ATTCCTCCTG AGGTAAGCTT CTGCACCAAT CTCGAATACT TGGGATTGTA CAATAATTAT 
CTGAGAGGAG AGATTCCAAG TGAGATATTC TCTCTACCAG CTTTGAAGTA TCTTTATTTG 
CACACAAACA ACTTCACCGG TTCTTTACCA GATTTTTCCC CTTCCTGTGC AATATCTGAT 
CTTTGGATTC ATCAAAATGC CTTTTCTGGA TCTTTGCCTT ATACTTTTAG CAATTGCCAC 
AACCTTACTA CGCTTATTGC ATCTTATAAC AACTTTGGAG GGGTTATTTC ATTAGAGATC 
TTCGAGGGTC TTTTGCAACT TGAGATTCTC TATCTTGATG AAAACAATCT TGAAGGGGAA 
ATACCGGAGA CTTTGTGGGG CCTTGAAAGA TTGCAAGAGC TTGTTCTTTC CGGGAACAAG 
CTGAATGGAA GTATACCTGA AAGAATTGCT CAATGCCATT TGCTAGCAGT TATTGCCCTC 
TCAAGTAATA AGCTGGTGGG TCAGATTCCT CATTCAATTG GAGATTTAAA ATATTTGAAC 
AATTTGTTCC TCTTTGATAA CATGCTTGAT GGCTCACTAC CTCCTCAGCT TGGAAACTGC 
ACTTCACTTG TTGAACTTAA GCTTCAGAAT AACTTCATCG GAGGGACAAT TCCTCCAGAA 
ATTTGCAATC TAGAAAATCT TGAGGTCCTT TTTCTGTTCA ATAATCTTAT CAGAGGCCAT 
ATTCCTCTAC AGATTGGTAG AATGAGTAGT CTTATGGAGT TGGCTCTGTA TAACAATAAC 
TTGACTGGTA GAATCCCACT AGGAATAACC CATTTGAAAA AGCTCACATT CCTGTCCTTA 
GCTCATAATA ATCTCTCTGG AGAGTTGCCA TCGGAGCTTG GCAAGGATAA TTCTCCTGGT 
CTACTTAAAT TGGATTTAAC TGGGAATAAG CTATATGGAC CAATCCCTTC TAGTATATGT 
ATAGGCAATA ATCTTTCAGT TTTGGCATTA GGGAACAACC ATTTTAATGG AAGTTTTCCA 
GTTGGCATTG AAAAATGCTC TTCTCTTAGA AGGGTGATCA TTAGTAACAA TCTCTTGCAA 
GGGACTATAC CGGCTGATTT GGGTAATAAC TCTGGAATTT CTTTCTTAGA AGTTCGAGGC 
AACTTGCTTC AAGGAACAAT ACCTCCAATG TTGGGACATT GGAGCAATTT ATCCATGCTT 
GATTTTGCAG ATAATAGATT TTCTGGAACT ATACCTCTGG AGCTTGGTAA ACTAGAGGCT 
CTACAAGTTT TGAGACTTTC TTCAAATAGG TTGGCTGGAA TGATCCCTTC TGAGCTGGGA 
CATTGCACGG AATTGATTGA ACTGGACCTC AGCAAGAACG ATCTTTCAGG AAGCATTCCT 
TTAAAGATCA TCTCAGTTGC AAAAATGCAA AGTCTTCACC TTGAAGAGAA CAAATTCAGT 
GGAATTATTC CTGACACTTT CTCTTCCCCT CATAGTCTGT TTGAGCTGCA GCTTGGAAGC 
AACATATTAG AAGGCCCTAT TCCTTGCAGT TTGGGTAAAC TTCATCATTT TAGTTCAATT 
CTCAATTTGA GCAATAATAG GCTCTCTGGT AATATCCCAC AATGCCTGGG CAATCTAGAC 
AAGTTGCAAA TTCTTGACCT ATCAAGTAAT GGTTTCTCTG GTGAAATACC AACTGCACTA 
AACAGTATGA TCTCCCTTAC CTTTGTGAAC ATATCGTTCA ATACCCTCTC TGGAAAACTC 
CCTTCTGCTT GGTTGAAGTT GGTGGCTTCA TATCCAGGAT CTTTCCTTGG AAACCCAGGA 
CTTTGCTTGC TTGGTAATGA AGAAAAGTAC TGTGAAGAAG CTAGAAATGT GGGTAAGAGA 
GGCCAAGTGC TGTCTGGAGT GATTGTAGGT GTAGTAATCT CAGTGTCACT ACTCTGTGCT 
CTAATTTACA TACTAATCGC TAGAGGCTTT GGGAAGAAAT CAACTCCTGA TCAGTCTCTC 
CTTCATGATT GTCGATCAGT AACTGAAGAT TTACCTCAGG ACTTACAATT TGAAGATATT 
ATGCGTGCCA CACAAGACCC AAATGACAAG TATGTGATCG GGAGAGGCAA AGATGGGACA 
GTCTACCGCA CAGAATCTGC AAACTCCAGA AAGCATTGGG CAGTCAAGAA GGTGGACTTG 
TCCAAGACAA GTTTTGTAAT TGAGACAAAG ACTTTAAACT TAATTAGGCA TCGGAATGTG 
GTGAGGATGG CAGGGTATTG CATGAAAGAT GGATATGGTT ACATTGTCAC AGAGTACGTG 
CCAGGAGGGA CCCTTTTTGA TGTACTGCAT AGAAGTGAAC CCCAGTTGGT TTTAGACTGG 
GACACCAGAT ATCGTATTGC TTTCAGCATT GCACAAGGCC TTTCTTACCT TCACCATGAC 
TGTGTGCCAC AAGTTATCCA TCGAGATATC AAATCTGACA ACATTCTACT GGACTCTCAA 
CTGGAACCAA AGATAGGAGA TTTCGGGATA GCAAAATTGG TTAATGATTC TGATCCAAGC 
TCCACAAGGT CTGCCATTGT AGGAACATTA GGCTACATCG CACCAGAGAA TGCGTATTCA 
ACTCGGTTGA CAGAGAAATG TGATGTATAT AGTTACGGAG TCATTCTGCT GGAGCTCCTT 
TGCAGAAGAT TGCCAGTTGA CCCTTGCTTT GAAGAAGGCT TGGATATTGT CTCCTGGTCA 
AAGAAGAAGC TGCAGGAAAA TGATGAGTTC ATTGGCTTCT TTGATGAGGA AATCTGTTAC 
TGGGAAAGAG ATGAACAACA AAAGGCATTG AAGTTGTTGG ACTTGGCGCT TGAGTGCACT 
GAACAAGTGG CGGATGCAAG ACCCTCCATG AGAGATGTAG TAGCATTCCT TATCAAGTTG 
AACGATAAGC ATGCAACCAG TTCAAGTAAT AGACAAAGTT CAAGGTTATA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_018822324.1
CDS	201..3611
Translation	MFLLFSYYVLSFIGFLLVNSVSLPPSAIYLIEFRHSLPKHSQHLLLWNESNSPSSPCQWPGVHCYPKNSFQVKVLNLSGFGLSGVLNNSISHLCHHKRLLSLDLSGNSFSGEIPLLLGNCAQLNTILLNDNDFRGPIPPELFGSKRLSRLDLGYNSLSGNIPPEVSFCTNLEYLGLYNNYLRGEIPSEIFSLPALKYLYLHTNNFTGSLPDFSPSCAISDLWIHQNAFSGSLPYTFSNCHNLTTLIASYNNFGGVISLEIFEGLLQLEILYLDENNLEGEIPETLWGLERLQELVLSGNKLNGSIPERIAQCHLLAVIALSSNKLVGQIPHSIGDLKYLNNLFLFDNMLDGSLPPQLGNCTSLVELKLQNNFIGGTIPPEICNLENLEVLFLFNNLIRGHIPLQIGRMSSLMELALYNNNLTGRIPLGITHLKKLTFLSLAHNNLSGELPSELGKDNSPGLLKLDLTGNKLYGPIPSSICIGNNLSVLALGNNHFNGSFPVGIEKCSSLRRVIISNNLLQGTIPADLGNNSGISFLEVRGNLLQGTIPPMLGHWSNLSMLDFADNRFSGTIPLELGKLEALQVLRLSSNRLAGMIPSELGHCTELIELDLSKNDLSGSIPLKIISVAKMQSLHLEENKFSGIIPDTFSSPHSLFELQLGSNILEGPIPCSLGKLHHFSSILNLSNNRLSGNIPQCLGNLDKLQILDLSSNGFSGEIPTALNSMISLTFVNISFNTLSGKLPSAWLKLVASYPGSFLGNPGLCLLGNEEKYCEEARNVGKRGQVLSGVIVGVVISVSLLCALIYILIARGFGKKSTPDQSLLHDCRSVTEDLPQDLQFEDIMRATQDPNDKYVIGRGKDGTVYRTESANSRKHWAVKKVDLSKTSFVIETKTLNLIRHRNVVRMAGYCMKDGYGYIVTEYVPGGTLFDVLHRSEPQLVLDWDTRYRIAFSIAQGLSYLHHDCVPQVIHRDIKSDNILLDSQLEPKIGDFGIAKLVNDSDPSSTRSAIVGTLGYIAPENAYSTRLTEKCDVYSYGVILLELLCRRLPVDPCFEEGLDIVSWSKKKLQENDEFIGFFDEEICYWERDEQQKALKLLDLALECTEQVADARPSMRDVVAFLIKLNDKHATSSSNRQSSRL

Target ORF information:

RefSeq Version	XM_018966779.1
Organism	Juglans regia(English walnut)
Definition	Juglans regia probable leucine-rich repeat receptor-like protein kinase At5g63930 (LOC108992272), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_018966779.1

ORF Insert Sequence:

ATGTTCTTGC TGTTTTCATA CTATGTTCTT TCCTTTATTG GGTTTCTGTT GGTTAACTCA 
GTTTCATTGC CTCCATCAGC CATATACCTT ATTGAATTCC GCCATAGCCT CCCCAAACAT 
TCTCAGCATC TCCTGCTATG GAACGAGTCC AATTCACCTT CCTCACCTTG CCAATGGCCA 
GGAGTTCATT GCTATCCCAA GAACAGCTTT CAAGTCAAGG TCTTAAACCT CTCAGGCTTT 
GGACTCTCTG GTGTCTTGAA CAACTCGATT TCCCATCTCT GCCACCATAA ACGTTTGCTC 
TCTCTTGACC TCAGTGGCAA CAGTTTTAGT GGTGAAATCC CCCTGCTGCT TGGAAACTGT 
GCTCAACTAA ATACCATTCT TCTCAACGAC AATGACTTCA GAGGGCCAAT CCCACCTGAG 
CTTTTCGGAT CAAAGCGGCT TTCACGGCTT GATTTGGGAT ATAATTCTCT CTCTGGTAAT 
ATTCCTCCTG AGGTAAGCTT CTGCACCAAT CTCGAATACT TGGGATTGTA CAATAATTAT 
CTGAGAGGAG AGATTCCAAG TGAGATATTC TCTCTACCAG CTTTGAAGTA TCTTTATTTG 
CACACAAACA ACTTCACCGG TTCTTTACCA GATTTTTCCC CTTCCTGTGC AATATCTGAT 
CTTTGGATTC ATCAAAATGC CTTTTCTGGA TCTTTGCCTT ATACTTTTAG CAATTGCCAC 
AACCTTACTA CGCTTATTGC ATCTTATAAC AACTTTGGAG GGGTTATTTC ATTAGAGATC 
TTCGAGGGTC TTTTGCAACT TGAGATTCTC TATCTTGATG AAAACAATCT TGAAGGGGAA 
ATACCGGAGA CTTTGTGGGG CCTTGAAAGA TTGCAAGAGC TTGTTCTTTC CGGGAACAAG 
CTGAATGGAA GTATACCTGA AAGAATTGCT CAATGCCATT TGCTAGCAGT TATTGCCCTC 
TCAAGTAATA AGCTGGTGGG TCAGATTCCT CATTCAATTG GAGATTTAAA ATATTTGAAC 
AATTTGTTCC TCTTTGATAA CATGCTTGAT GGCTCACTAC CTCCTCAGCT TGGAAACTGC 
ACTTCACTTG TTGAACTTAA GCTTCAGAAT AACTTCATCG GAGGGACAAT TCCTCCAGAA 
ATTTGCAATC TAGAAAATCT TGAGGTCCTT TTTCTGTTCA ATAATCTTAT CAGAGGCCAT 
ATTCCTCTAC AGATTGGTAG AATGAGTAGT CTTATGGAGT TGGCTCTGTA TAACAATAAC 
TTGACTGGTA GAATCCCACT AGGAATAACC CATTTGAAAA AGCTCACATT CCTGTCCTTA 
GCTCATAATA ATCTCTCTGG AGAGTTGCCA TCGGAGCTTG GCAAGGATAA TTCTCCTGGT 
CTACTTAAAT TGGATTTAAC TGGGAATAAG CTATATGGAC CAATCCCTTC TAGTATATGT 
ATAGGCAATA ATCTTTCAGT TTTGGCATTA GGGAACAACC ATTTTAATGG AAGTTTTCCA 
GTTGGCATTG AAAAATGCTC TTCTCTTAGA AGGGTGATCA TTAGTAACAA TCTCTTGCAA 
GGGACTATAC CGGCTGATTT GGGTAATAAC TCTGGAATTT CTTTCTTAGA AGTTCGAGGC 
AACTTGCTTC AAGGAACAAT ACCTCCAATG TTGGGACATT GGAGCAATTT ATCCATGCTT 
GATTTTGCAG ATAATAGATT TTCTGGAACT ATACCTCTGG AGCTTGGTAA ACTAGAGGCT 
CTACAAGTTT TGAGACTTTC TTCAAATAGG TTGGCTGGAA TGATCCCTTC TGAGCTGGGA 
CATTGCACGG AATTGATTGA ACTGGACCTC AGCAAGAACG ATCTTTCAGG AAGCATTCCT 
TTAAAGATCA TCTCAGTTGC AAAAATGCAA AGTCTTCACC TTGAAGAGAA CAAATTCAGT 
GGAATTATTC CTGACACTTT CTCTTCCCCT CATAGTCTGT TTGAGCTGCA GCTTGGAAGC 
AACATATTAG AAGGCCCTAT TCCTTGCAGT TTGGGTAAAC TTCATCATTT TAGTTCAATT 
CTCAATTTGA GCAATAATAG GCTCTCTGGT AATATCCCAC AATGCCTGGG CAATCTAGAC 
AAGTTGCAAA TTCTTGACCT ATCAAGTAAT GGTTTCTCTG GTGAAATACC AACTGCACTA 
AACAGTATGA TCTCCCTTAC CTTTGTGAAC ATATCGTTCA ATACCCTCTC TGGAAAACTC 
CCTTCTGCTT GGTTGAAGTT GGTGGCTTCA TATCCAGGAT CTTTCCTTGG AAACCCAGGA 
CTTTGCTTGC TTGGTAATGA AGAAAAGTAC TGTGAAGAAG CTAGAAATGT GGGTAAGAGA 
GGCCAAGTGC TGTCTGGAGT GATTGTAGGT GTAGTAATCT CAGTGTCACT ACTCTGTGCT 
CTAATTTACA TACTAATCGC TAGAGGCTTT GGGAAGAAAT CAACTCCTGA TCAGTCTCTC 
CTTCATGATT GTCGATCAGT AACTGAAGAT TTACCTCAGG ACTTACAATT TGAAGATATT 
ATGCGTGCCA CACAAGACCC AAATGACAAG TATGTGATCG GGAGAGGCAA AGATGGGACA 
GTCTACCGCA CAGAATCTGC AAACTCCAGA AAGCATTGGG CAGTCAAGAA GGTGGACTTG 
TCCAAGACAA GTTTTGTAAT TGAGACAAAG ACTTTAAACT TAATTAGGCA TCGGAATGTG 
GTGAGGATGG CAGGGTATTG CATGAAAGAT GGATATGGTT ACATTGTCAC AGAGTACGTG 
CCAGGAGGGA CCCTTTTTGA TGTACTGCAT AGAAGTGAAC CCCAGTTGGT TTTAGACTGG 
GACACCAGAT ATCGTATTGC TTTCAGCATT GCACAAGGCC TTTCTTACCT TCACCATGAC 
TGTGTGCCAC AAGTTATCCA TCGAGATATC AAATCTGACA ACATTCTACT GGACTCTCAA 
CTGGAACCAA AGATAGGAGA TTTCGGGATA GCAAAATTGG TTAATGATTC TGATCCAAGC 
TCCACAAGGT CTGCCATTGT AGGAACATTA GGCTACATCG CACCAGAGAA TGCGTATTCA 
ACTCGGTTGA CAGAGAAATG TGATGTATAT AGTTACGGAG TCATTCTGCT GGAGCTCCTT 
TGCAGAAGAT TGCCAGTTGA CCCTTGCTTT GAAGAAGGCT TGGATATTGT CTCCTGGTCA 
AAGAAGAAGC TGCAGGAAAA TGATGAGTTC ATTGGCTTCT TTGATGAGGA AATCTGTTAC 
TGGGAAAGAG ATGAACAACA AAAGGCATTG AAGTTGTTGG ACTTGGCGCT TGAGTGCACT 
GAACAAGTGG CGGATGCAAG ACCCTCCATG AGAGATGTAG TAGCATTCCT TATCAAGTTG 
AACGATAAGC ATGCAACCAG TTCAAGTAAT AGACAAAGTT CAAGGTTATA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC108992272 ( XM_018966780.1 ) cDNA ORF clone, Juglans regia(English walnut) -> XP_018822325.1 Juglans regia probable leucine-rich repeat receptor-like protein kinase At5g63930 (LOC108992272), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OJu35601
Clone ID Related Accession (Same CDS sequence)	XM_018966780.1
Accession Version	XM_018966780.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3294bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-11-02
Organism	Juglans regia(English walnut)
Product	probable leucine-rich repeat receptor-like protein kinase At5g63930 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_017443529.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Juglans regia Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTTCTTTC CTTTATTGGG TTTCTGTTGG TTAACTCAGT TTCATTGCCT CCATCAGCCA 
TATACCTTAT TGAATTCCGC CATAGCCTCC CCAAACATTC TCAGCATCTC CTGCTATGGA 
ACGAGTCCAA TTCACCTTCC TCACCTTGCC AATGGCCAGG AGTTCATTGC TATCCCAAGA 
ACAGCTTTCA AGTCAAGTGG CAACAGTTTT AGTGGTGAAA TCCCCCTGCT GCTTGGAAAC 
TGTGCTCAAC TAAATACCAT TCTTCTCAAC GACAATGACT TCAGAGGGCC AATCCCACCT 
GAGCTTTTCG GATCAAAGCG GCTTTCACGG CTTGATTTGG GATATAATTC TCTCTCTGGT 
AATATTCCTC CTGAGGTAAG CTTCTGCACC AATCTCGAAT ACTTGGGATT GTACAATAAT 
TATCTGAGAG GAGAGATTCC AAGTGAGATA TTCTCTCTAC CAGCTTTGAA GTATCTTTAT 
TTGCACACAA ACAACTTCAC CGGTTCTTTA CCAGATTTTT CCCCTTCCTG TGCAATATCT 
GATCTTTGGA TTCATCAAAA TGCCTTTTCT GGATCTTTGC CTTATACTTT TAGCAATTGC 
CACAACCTTA CTACGCTTAT TGCATCTTAT AACAACTTTG GAGGGGTTAT TTCATTAGAG 
ATCTTCGAGG GTCTTTTGCA ACTTGAGATT CTCTATCTTG ATGAAAACAA TCTTGAAGGG 
GAAATACCGG AGACTTTGTG GGGCCTTGAA AGATTGCAAG AGCTTGTTCT TTCCGGGAAC 
AAGCTGAATG GAAGTATACC TGAAAGAATT GCTCAATGCC ATTTGCTAGC AGTTATTGCC 
CTCTCAAGTA ATAAGCTGGT GGGTCAGATT CCTCATTCAA TTGGAGATTT AAAATATTTG 
AACAATTTGT TCCTCTTTGA TAACATGCTT GATGGCTCAC TACCTCCTCA GCTTGGAAAC 
TGCACTTCAC TTGTTGAACT TAAGCTTCAG AATAACTTCA TCGGAGGGAC AATTCCTCCA 
GAAATTTGCA ATCTAGAAAA TCTTGAGGTC CTTTTTCTGT TCAATAATCT TATCAGAGGC 
CATATTCCTC TACAGATTGG TAGAATGAGT AGTCTTATGG AGTTGGCTCT GTATAACAAT 
AACTTGACTG GTAGAATCCC ACTAGGAATA ACCCATTTGA AAAAGCTCAC ATTCCTGTCC 
TTAGCTCATA ATAATCTCTC TGGAGAGTTG CCATCGGAGC TTGGCAAGGA TAATTCTCCT 
GGTCTACTTA AATTGGATTT AACTGGGAAT AAGCTATATG GACCAATCCC TTCTAGTATA 
TGTATAGGCA ATAATCTTTC AGTTTTGGCA TTAGGGAACA ACCATTTTAA TGGAAGTTTT 
CCAGTTGGCA TTGAAAAATG CTCTTCTCTT AGAAGGGTGA TCATTAGTAA CAATCTCTTG 
CAAGGGACTA TACCGGCTGA TTTGGGTAAT AACTCTGGAA TTTCTTTCTT AGAAGTTCGA 
GGCAACTTGC TTCAAGGAAC AATACCTCCA ATGTTGGGAC ATTGGAGCAA TTTATCCATG 
CTTGATTTTG CAGATAATAG ATTTTCTGGA ACTATACCTC TGGAGCTTGG TAAACTAGAG 
GCTCTACAAG TTTTGAGACT TTCTTCAAAT AGGTTGGCTG GAATGATCCC TTCTGAGCTG 
GGACATTGCA CGGAATTGAT TGAACTGGAC CTCAGCAAGA ACGATCTTTC AGGAAGCATT 
CCTTTAAAGA TCATCTCAGT TGCAAAAATG CAAAGTCTTC ACCTTGAAGA GAACAAATTC 
AGTGGAATTA TTCCTGACAC TTTCTCTTCC CCTCATAGTC TGTTTGAGCT GCAGCTTGGA 
AGCAACATAT TAGAAGGCCC TATTCCTTGC AGTTTGGGTA AACTTCATCA TTTTAGTTCA 
ATTCTCAATT TGAGCAATAA TAGGCTCTCT GGTAATATCC CACAATGCCT GGGCAATCTA 
GACAAGTTGC AAATTCTTGA CCTATCAAGT AATGGTTTCT CTGGTGAAAT ACCAACTGCA 
CTAAACAGTA TGATCTCCCT TACCTTTGTG AACATATCGT TCAATACCCT CTCTGGAAAA 
CTCCCTTCTG CTTGGTTGAA GTTGGTGGCT TCATATCCAG GATCTTTCCT TGGAAACCCA 
GGACTTTGCT TGCTTGGTAA TGAAGAAAAG TACTGTGAAG AAGCTAGAAA TGTGGGTAAG 
AGAGGCCAAG TGCTGTCTGG AGTGATTGTA GGTGTAGTAA TCTCAGTGTC ACTACTCTGT 
GCTCTAATTT ACATACTAAT CGCTAGAGGC TTTGGGAAGA AATCAACTCC TGATCAGTCT 
CTCCTTCATG ATTGTCGATC AGTAACTGAA GATTTACCTC AGGACTTACA ATTTGAAGAT 
ATTATGCGTG CCACACAAGA CCCAAATGAC AAGTATGTGA TCGGGAGAGG CAAAGATGGG 
ACAGTCTACC GCACAGAATC TGCAAACTCC AGAAAGCATT GGGCAGTCAA GAAGGTGGAC 
TTGTCCAAGA CAAGTTTTGT AATTGAGACA AAGACTTTAA ACTTAATTAG GCATCGGAAT 
GTGGTGAGGA TGGCAGGGTA TTGCATGAAA GATGGATATG GTTACATTGT CACAGAGTAC 
GTGCCAGGAG GGACCCTTTT TGATGTACTG CATAGAAGTG AACCCCAGTT GGTTTTAGAC 
TGGGACACCA GATATCGTAT TGCTTTCAGC ATTGCACAAG GCCTTTCTTA CCTTCACCAT 
GACTGTGTGC CACAAGTTAT CCATCGAGAT ATCAAATCTG ACAACATTCT ACTGGACTCT 
CAACTGGAAC CAAAGATAGG AGATTTCGGG ATAGCAAAAT TGGTTAATGA TTCTGATCCA 
AGCTCCACAA GGTCTGCCAT TGTAGGAACA TTAGGCTACA TCGCACCAGA GAATGCGTAT 
TCAACTCGGT TGACAGAGAA ATGTGATGTA TATAGTTACG GAGTCATTCT GCTGGAGCTC 
CTTTGCAGAA GATTGCCAGT TGACCCTTGC TTTGAAGAAG GCTTGGATAT TGTCTCCTGG 
TCAAAGAAGA AGCTGCAGGA AAATGATGAG TTCATTGGCT TCTTTGATGA GGAAATCTGT 
TACTGGGAAA GAGATGAACA ACAAAAGGCA TTGAAGTTGT TGGACTTGGC GCTTGAGTGC 
ACTGAACAAG TGGCGGATGC AAGACCCTCC ATGAGAGATG TAGTAGCATT CCTTATCAAG 
TTGAACGATA AGCATGCAAC CAGTTCAAGT AATAGACAAA GTTCAAGGTT ATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_018822325.1
CDS	223..3516
Translation	MFFPLLGFCWLTQFHCLHQPYTLLNSAIASPNILSISCYGTSPIHLPHLANGQEFIAIPRTAFKSSGNSFSGEIPLLLGNCAQLNTILLNDNDFRGPIPPELFGSKRLSRLDLGYNSLSGNIPPEVSFCTNLEYLGLYNNYLRGEIPSEIFSLPALKYLYLHTNNFTGSLPDFSPSCAISDLWIHQNAFSGSLPYTFSNCHNLTTLIASYNNFGGVISLEIFEGLLQLEILYLDENNLEGEIPETLWGLERLQELVLSGNKLNGSIPERIAQCHLLAVIALSSNKLVGQIPHSIGDLKYLNNLFLFDNMLDGSLPPQLGNCTSLVELKLQNNFIGGTIPPEICNLENLEVLFLFNNLIRGHIPLQIGRMSSLMELALYNNNLTGRIPLGITHLKKLTFLSLAHNNLSGELPSELGKDNSPGLLKLDLTGNKLYGPIPSSICIGNNLSVLALGNNHFNGSFPVGIEKCSSLRRVIISNNLLQGTIPADLGNNSGISFLEVRGNLLQGTIPPMLGHWSNLSMLDFADNRFSGTIPLELGKLEALQVLRLSSNRLAGMIPSELGHCTELIELDLSKNDLSGSIPLKIISVAKMQSLHLEENKFSGIIPDTFSSPHSLFELQLGSNILEGPIPCSLGKLHHFSSILNLSNNRLSGNIPQCLGNLDKLQILDLSSNGFSGEIPTALNSMISLTFVNISFNTLSGKLPSAWLKLVASYPGSFLGNPGLCLLGNEEKYCEEARNVGKRGQVLSGVIVGVVISVSLLCALIYILIARGFGKKSTPDQSLLHDCRSVTEDLPQDLQFEDIMRATQDPNDKYVIGRGKDGTVYRTESANSRKHWAVKKVDLSKTSFVIETKTLNLIRHRNVVRMAGYCMKDGYGYIVTEYVPGGTLFDVLHRSEPQLVLDWDTRYRIAFSIAQGLSYLHHDCVPQVIHRDIKSDNILLDSQLEPKIGDFGIAKLVNDSDPSSTRSAIVGTLGYIAPENAYSTRLTEKCDVYSYGVILLELLCRRLPVDPCFEEGLDIVSWSKKKLQENDEFIGFFDEEICYWERDEQQKALKLLDLALECTEQVADARPSMRDVVAFLIKLNDKHATSSSNRQSSRL

Target ORF information:

RefSeq Version	XM_018966780.1
Organism	Juglans regia(English walnut)
Definition	Juglans regia probable leucine-rich repeat receptor-like protein kinase At5g63930 (LOC108992272), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_018966780.1

ORF Insert Sequence:

ATGTTCTTTC CTTTATTGGG TTTCTGTTGG TTAACTCAGT TTCATTGCCT CCATCAGCCA 
TATACCTTAT TGAATTCCGC CATAGCCTCC CCAAACATTC TCAGCATCTC CTGCTATGGA 
ACGAGTCCAA TTCACCTTCC TCACCTTGCC AATGGCCAGG AGTTCATTGC TATCCCAAGA 
ACAGCTTTCA AGTCAAGTGG CAACAGTTTT AGTGGTGAAA TCCCCCTGCT GCTTGGAAAC 
TGTGCTCAAC TAAATACCAT TCTTCTCAAC GACAATGACT TCAGAGGGCC AATCCCACCT 
GAGCTTTTCG GATCAAAGCG GCTTTCACGG CTTGATTTGG GATATAATTC TCTCTCTGGT 
AATATTCCTC CTGAGGTAAG CTTCTGCACC AATCTCGAAT ACTTGGGATT GTACAATAAT 
TATCTGAGAG GAGAGATTCC AAGTGAGATA TTCTCTCTAC CAGCTTTGAA GTATCTTTAT 
TTGCACACAA ACAACTTCAC CGGTTCTTTA CCAGATTTTT CCCCTTCCTG TGCAATATCT 
GATCTTTGGA TTCATCAAAA TGCCTTTTCT GGATCTTTGC CTTATACTTT TAGCAATTGC 
CACAACCTTA CTACGCTTAT TGCATCTTAT AACAACTTTG GAGGGGTTAT TTCATTAGAG 
ATCTTCGAGG GTCTTTTGCA ACTTGAGATT CTCTATCTTG ATGAAAACAA TCTTGAAGGG 
GAAATACCGG AGACTTTGTG GGGCCTTGAA AGATTGCAAG AGCTTGTTCT TTCCGGGAAC 
AAGCTGAATG GAAGTATACC TGAAAGAATT GCTCAATGCC ATTTGCTAGC AGTTATTGCC 
CTCTCAAGTA ATAAGCTGGT GGGTCAGATT CCTCATTCAA TTGGAGATTT AAAATATTTG 
AACAATTTGT TCCTCTTTGA TAACATGCTT GATGGCTCAC TACCTCCTCA GCTTGGAAAC 
TGCACTTCAC TTGTTGAACT TAAGCTTCAG AATAACTTCA TCGGAGGGAC AATTCCTCCA 
GAAATTTGCA ATCTAGAAAA TCTTGAGGTC CTTTTTCTGT TCAATAATCT TATCAGAGGC 
CATATTCCTC TACAGATTGG TAGAATGAGT AGTCTTATGG AGTTGGCTCT GTATAACAAT 
AACTTGACTG GTAGAATCCC ACTAGGAATA ACCCATTTGA AAAAGCTCAC ATTCCTGTCC 
TTAGCTCATA ATAATCTCTC TGGAGAGTTG CCATCGGAGC TTGGCAAGGA TAATTCTCCT 
GGTCTACTTA AATTGGATTT AACTGGGAAT AAGCTATATG GACCAATCCC TTCTAGTATA 
TGTATAGGCA ATAATCTTTC AGTTTTGGCA TTAGGGAACA ACCATTTTAA TGGAAGTTTT 
CCAGTTGGCA TTGAAAAATG CTCTTCTCTT AGAAGGGTGA TCATTAGTAA CAATCTCTTG 
CAAGGGACTA TACCGGCTGA TTTGGGTAAT AACTCTGGAA TTTCTTTCTT AGAAGTTCGA 
GGCAACTTGC TTCAAGGAAC AATACCTCCA ATGTTGGGAC ATTGGAGCAA TTTATCCATG 
CTTGATTTTG CAGATAATAG ATTTTCTGGA ACTATACCTC TGGAGCTTGG TAAACTAGAG 
GCTCTACAAG TTTTGAGACT TTCTTCAAAT AGGTTGGCTG GAATGATCCC TTCTGAGCTG 
GGACATTGCA CGGAATTGAT TGAACTGGAC CTCAGCAAGA ACGATCTTTC AGGAAGCATT 
CCTTTAAAGA TCATCTCAGT TGCAAAAATG CAAAGTCTTC ACCTTGAAGA GAACAAATTC 
AGTGGAATTA TTCCTGACAC TTTCTCTTCC CCTCATAGTC TGTTTGAGCT GCAGCTTGGA 
AGCAACATAT TAGAAGGCCC TATTCCTTGC AGTTTGGGTA AACTTCATCA TTTTAGTTCA 
ATTCTCAATT TGAGCAATAA TAGGCTCTCT GGTAATATCC CACAATGCCT GGGCAATCTA 
GACAAGTTGC AAATTCTTGA CCTATCAAGT AATGGTTTCT CTGGTGAAAT ACCAACTGCA 
CTAAACAGTA TGATCTCCCT TACCTTTGTG AACATATCGT TCAATACCCT CTCTGGAAAA 
CTCCCTTCTG CTTGGTTGAA GTTGGTGGCT TCATATCCAG GATCTTTCCT TGGAAACCCA 
GGACTTTGCT TGCTTGGTAA TGAAGAAAAG TACTGTGAAG AAGCTAGAAA TGTGGGTAAG 
AGAGGCCAAG TGCTGTCTGG AGTGATTGTA GGTGTAGTAA TCTCAGTGTC ACTACTCTGT 
GCTCTAATTT ACATACTAAT CGCTAGAGGC TTTGGGAAGA AATCAACTCC TGATCAGTCT 
CTCCTTCATG ATTGTCGATC AGTAACTGAA GATTTACCTC AGGACTTACA ATTTGAAGAT 
ATTATGCGTG CCACACAAGA CCCAAATGAC AAGTATGTGA TCGGGAGAGG CAAAGATGGG 
ACAGTCTACC GCACAGAATC TGCAAACTCC AGAAAGCATT GGGCAGTCAA GAAGGTGGAC 
TTGTCCAAGA CAAGTTTTGT AATTGAGACA AAGACTTTAA ACTTAATTAG GCATCGGAAT 
GTGGTGAGGA TGGCAGGGTA TTGCATGAAA GATGGATATG GTTACATTGT CACAGAGTAC 
GTGCCAGGAG GGACCCTTTT TGATGTACTG CATAGAAGTG AACCCCAGTT GGTTTTAGAC 
TGGGACACCA GATATCGTAT TGCTTTCAGC ATTGCACAAG GCCTTTCTTA CCTTCACCAT 
GACTGTGTGC CACAAGTTAT CCATCGAGAT ATCAAATCTG ACAACATTCT ACTGGACTCT 
CAACTGGAAC CAAAGATAGG AGATTTCGGG ATAGCAAAAT TGGTTAATGA TTCTGATCCA 
AGCTCCACAA GGTCTGCCAT TGTAGGAACA TTAGGCTACA TCGCACCAGA GAATGCGTAT 
TCAACTCGGT TGACAGAGAA ATGTGATGTA TATAGTTACG GAGTCATTCT GCTGGAGCTC 
CTTTGCAGAA GATTGCCAGT TGACCCTTGC TTTGAAGAAG GCTTGGATAT TGTCTCCTGG 
TCAAAGAAGA AGCTGCAGGA AAATGATGAG TTCATTGGCT TCTTTGATGA GGAAATCTGT 
TACTGGGAAA GAGATGAACA ACAAAAGGCA TTGAAGTTGT TGGACTTGGC GCTTGAGTGC 
ACTGAACAAG TGGCGGATGC AAGACCCTCC ATGAGAGATG TAGTAGCATT CCTTATCAAG 
TTGAACGATA AGCATGCAAC CAGTTCAAGT AATAGACAAA GTTCAAGGTT ATAG