PI3 kinase p150 Antibody, mAb, Rabbit
PI3 Kinase P150 Antibody, MAb, Rabbit

Western blot analysis of PI 3 Kinase R4 expression in (1) Jurkat cell lysate; (2) Molt-4 cell lysate.

PI3 kinase p150 Antibody, mAb, Rabbit

Regulatory subunit of the PI3K complex that mediates formation of phosphatidylinositol 3-phosphate; different complex forms are believed to play a role in multiple membrane trafficking pathways: PI3KC3-C1 is involved in initiation of autophagosomes and PI3KC3-C2 in maturation of autophagosomes and endocytosis. Involved in regulation of degradative endocytic trafficking and cytokinesis, probably in the context of PI3KC3-C2.
A05222
¥37,309.00

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Overview
Specificity This product is specific to PI3 kinase p150
Host Species Rabbit
Immunogen A synthesized peptide derived from human PI3 kinase p150
Species Reactivity Human; Mouse; Rat
Conjugate Unconjugated

Applications
Working concentrations for specific applications should be determined by the investigators. The appropriate concentrations may be affected by secondary antibody affinity, antigen concentration, the sensitivity of the method of detection, temperature, the length of the incubations, and other factors. The suitability of this antibody for applications other than those listed below has not been determined. The following concentration ranges are recommended starting points for this product.
Application Recommended Usage
Western Blot 1:1000-1:2000
Immunohistochemistry (IHC) 1:100-1:200
Immunocytochemistry/Immunofluorescence (ICC/IF) 1:50-1:200
Immunohistochemistry Frozen (IHF) 1:50-1:200
Flow Cytometry 1:20-1:100

Properties
Form Liquid
Storage Buffer Supplied in 10mM phosphate buffered saline , pH 7.4, 150mM sodium chloride, 0.05% BSA, 0.02% sodium azide and 50% glycerol.
Storage Instructions Store at -20°C. This product is stable for 1 year upon receipt, when handled and stored as instructed.
Purification Affinity-chromatography
Isotype Rabbit IgG
Clonality Monoclonal

Examples
  • PI3 Kinase P150 Antibody, MAb, Rabbit
    • PI3 Kinase P150 Antibody, MAb, Rabbit

    Western blot analysis of PI 3 Kinase R4 expression in (1) Jurkat cell lysate; (2) Molt-4 cell lysate.


Background
Target Background Regulatory subunit of the PI3K complex that mediates formation of phosphatidylinositol 3-phosphate; different complex forms are believed to play a role in multiple membrane trafficking pathways: PI3KC3-C1 is involved in initiation of autophagosomes and PI3KC3-C2 in maturation of autophagosomes and endocytosis. Involved in regulation of degradative endocytic trafficking and cytokinesis, probably in the context of PI3KC3-C2.
Synonyms P150; Phosphoinositide 3 kinase regulatory subunit 4; PI3 kinase p150 subunit; PIK3R4; VPS15;

For research use only. Not intended for human or animal clinical trials, therapeutic or diagnostic use.