p-Vimentin (S39) Antibody, mAb, Rabbit
P-Vimentin (S39) Antibody, MAb, Rabbit

Western blot analysis of Phospho-Vimentin (S39) expression in HeLa treated with Calyculin A cell lysate.

p-Vimentin (S39) Antibody, mAb, Rabbit

Vimentins are class-III intermediate filaments found in various non-epithelial cells, especially mesenchymal cells. Vimentin is attached to the nucleus, endoplasmic reticulum, and mitochondria, either laterally or terminally. Involved with LARP6 in the stabilization of type I collagen mRNAs for CO1A1 and CO1A2.
A05035
¥37,309.00

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Overview
Specificity This product is specific to p-Vimentin (S39)
Host Species Rabbit
Immunogen A synthesized peptide derived from human Vimentin around the phosphorylation site of S39
Species Reactivity Human; Mouse; Rat
Conjugate Unconjugated

Applications
Working concentrations for specific applications should be determined by the investigators. The appropriate concentrations may be affected by secondary antibody affinity, antigen concentration, the sensitivity of the method of detection, temperature, the length of the incubations, and other factors. The suitability of this antibody for applications other than those listed below has not been determined. The following concentration ranges are recommended starting points for this product.
Application Recommended Usage
Western Blot 1:1000-1:2000

Properties
Form Liquid
Storage Buffer Supplied in 10mM phosphate buffered saline , pH 7.4, 150mM sodium chloride, 0.05% BSA, 0.02% sodium azide and 50% glycerol.
Storage Instructions Store at -20°C. This product is stable for 1 year upon receipt, when handled and stored as instructed.
Purification Affinity-chromatography
Isotype Rabbit IgG
Clonality Monoclonal

Examples
  • P-Vimentin (S39) Antibody, MAb, Rabbit
    • P-Vimentin (S39) Antibody, MAb, Rabbit

    Western blot analysis of Phospho-Vimentin (S39) expression in HeLa treated with Calyculin A cell lysate.


Background
Target Background Vimentins are class-III intermediate filaments found in various non-epithelial cells, especially mesenchymal cells. Vimentin is attached to the nucleus, endoplasmic reticulum, and mitochondria, either laterally or terminally. Involved with LARP6 in the stabilization of type I collagen mRNAs for CO1A1 and CO1A2.
Synonyms VIM; VIME; Vimentin;

For research use only. Not intended for human or animal clinical trials, therapeutic or diagnostic use.