Glutamate dehydrogenase 1 Antibody, mAb, Rabbit
Glutamate Dehydrogenase 1 Antibody, MAb, Rabbit

Western blot analysis of GLUD1 expression in HepG2 cell lysate.

Glutamate dehydrogenase 1 Antibody, mAb, Rabbit

Mitochondrial glutamate dehydrogenase that catalyzes the conversion of L-glutamate into alpha-ketoglutarate. Plays a key role in glutamine anaplerosis by producing alpha-ketoglutarate, an important intermediate in the tricarboxylic acid cycle. Plays a role in insulin homeostasis.
A04253
¥37,309.00

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Overview
Specificity This product is specific to Glutamate dehydrogenase 1
Host Species Rabbit
Immunogen A synthesized peptide derived from human Glutamate dehydrogenase 1
Species Reactivity Human; Mouse; Rat
Conjugate Unconjugated

Applications
Working concentrations for specific applications should be determined by the investigators. The appropriate concentrations may be affected by secondary antibody affinity, antigen concentration, the sensitivity of the method of detection, temperature, the length of the incubations, and other factors. The suitability of this antibody for applications other than those listed below has not been determined. The following concentration ranges are recommended starting points for this product.
Application Recommended Usage
Western Blot 1:1000-1:2000
Immunohistochemistry (IHC) 1:100-1:200
Immunocytochemistry/Immunofluorescence (ICC/IF) 1:50-1:200
Immunohistochemistry Frozen (IHF) 1:50-1:200
Flow Cytometry 1:20-1:100

Properties
Form Liquid
Storage Buffer Supplied in 10mM phosphate buffered saline , pH 7.4, 150mM sodium chloride, 0.05% BSA, 0.02% sodium azide and 50% glycerol.
Storage Instructions Store at -20°C. This product is stable for 1 year upon receipt, when handled and stored as instructed.
Purification Affinity-chromatography
Isotype Rabbit IgG
Clonality Monoclonal

Examples
  • Glutamate Dehydrogenase 1 Antibody, MAb, Rabbit
    • Glutamate Dehydrogenase 1 Antibody, MAb, Rabbit

    Western blot analysis of GLUD1 expression in HepG2 cell lysate.


Background
Target Background Mitochondrial glutamate dehydrogenase that catalyzes the conversion of L-glutamate into alpha-ketoglutarate. Plays a key role in glutamine anaplerosis by producing alpha-ketoglutarate, an important intermediate in the tricarboxylic acid cycle. Plays a role in insulin homeostasis.
Synonyms GDH; Gdh-X; GDH1; GLUD; Glud1; Glud1a; Glud1b; Gludl; MRG-2;

For research use only. Not intended for human or animal clinical trials, therapeutic or diagnostic use.