TFIIE alpha Antibody, mAb, Rabbit
TFIIE Alpha Antibody, MAb, Rabbit

Western blot analysis of TFIIE alpha expression in HeLa cell lysate.

TFIIE alpha Antibody, mAb, Rabbit

Recruits TFIIH to the initiation complex and stimulates the RNA polymerase II C-terminal domain kinase and DNA-dependent ATPase activities of TFIIH. Both TFIIH and TFIIE are required for promoter clearance by RNA polymerase.
A04178
¥37,309.00

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Overview
Specificity This product is specific to TFIIE alpha
Host Species Rabbit
Immunogen A synthesized peptide derived from human TFIIE alpha
Species Reactivity Human; Mouse; Rat
Conjugate Unconjugated

Applications
Working concentrations for specific applications should be determined by the investigators. The appropriate concentrations may be affected by secondary antibody affinity, antigen concentration, the sensitivity of the method of detection, temperature, the length of the incubations, and other factors. The suitability of this antibody for applications other than those listed below has not been determined. The following concentration ranges are recommended starting points for this product.
Application Recommended Usage
Western Blot 1:1000-1:2000
Immunohistochemistry (IHC) 1:100-1:200
Immunocytochemistry/Immunofluorescence (ICC/IF) 1:50-1:200
Immunohistochemistry Frozen (IHF) 1:50-1:200

Properties
Form Liquid
Storage Buffer Supplied in 10mM phosphate buffered saline , pH 7.4, 150mM sodium chloride, 0.05% BSA, 0.02% sodium azide and 50% glycerol.
Storage Instructions Store at -20°C. This product is stable for 1 year upon receipt, when handled and stored as instructed.
Purification Affinity-chromatography
Isotype Rabbit IgG
Clonality Monoclonal

Examples
  • TFIIE Alpha Antibody, MAb, Rabbit
    • TFIIE Alpha Antibody, MAb, Rabbit

    Western blot analysis of TFIIE alpha expression in HeLa cell lysate.


Background
Target Background Recruits TFIIH to the initiation complex and stimulates the RNA polymerase II C-terminal domain kinase and DNA-dependent ATPase activities of TFIIH. Both TFIIH and TFIIE are required for promoter clearance by RNA polymerase.
Synonyms Gtf2e1; TF2E1; TFIIE alpha;

For research use only. Not intended for human or animal clinical trials, therapeutic or diagnostic use.