AKT1S1 Antibody, mAb, Rabbit
AKT1S1 Antibody, MAb, Rabbit

Western blot analysis of PRAS40 expression in (1) HeLa cell lysate; (2) RAW 264.7 cell lysate; (3) PC12 cell lysate.

AKT1S1 Antibody, mAb, Rabbit

Negative regulator of the mechanistic target of rapamycin complex 1 (mTORC1), an evolutionarily conserved central nutrient sensor that stimulates anabolic reactions and macromolecule biosynthesis to promote cellular biomass generation and growth.
A03862
¥37,309.00

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Overview
Specificity This product is specific to AKT1S1
Host Species Rabbit
Immunogen A synthesized peptide derived from human AKT1S1
Species Reactivity Human; Mouse; Rat
Conjugate Unconjugated

Applications
Working concentrations for specific applications should be determined by the investigators. The appropriate concentrations may be affected by secondary antibody affinity, antigen concentration, the sensitivity of the method of detection, temperature, the length of the incubations, and other factors. The suitability of this antibody for applications other than those listed below has not been determined. The following concentration ranges are recommended starting points for this product.
Application Recommended Usage
Western Blot 1:1000-1:2000
Immunoprecipitation (IP) 1:20-1:50

Properties
Form Liquid
Storage Buffer Supplied in 10mM phosphate buffered saline , pH 7.4, 150mM sodium chloride, 0.05% BSA, 0.02% sodium azide and 50% glycerol.
Storage Instructions Store at -20°C. This product is stable for 1 year upon receipt, when handled and stored as instructed.
Purification Affinity-chromatography
Isotype Rabbit IgG
Clonality Monoclonal

Examples
  • AKT1S1 Antibody, MAb, Rabbit
    • AKT1S1 Antibody, MAb, Rabbit

    Western blot analysis of PRAS40 expression in (1) HeLa cell lysate; (2) RAW 264.7 cell lysate; (3) PC12 cell lysate.


Background
Target Background Negative regulator of the mechanistic target of rapamycin complex 1 (mTORC1), an evolutionarily conserved central nutrient sensor that stimulates anabolic reactions and macromolecule biosynthesis to promote cellular biomass generation and growth.
Synonyms 40 kDa proline rich AKT substrate; AKT1 S1; Lobe; PRAS40; Proline rich akt substrate; Proline rich Akt substrate 40 kDa;

For research use only. Not intended for human or animal clinical trials, therapeutic or diagnostic use.