S100A1 Antibody, mAb, Rabbit
S100A1 Antibody, MAb, Rabbit

Western blot analysis of S100 expression in (1) Human skeletal muscle lysate; (2) RAW 264.7 cell lysate.

S100A1 Antibody, mAb, Rabbit

Weakly binds calcium but binds zinc very tightly-distinct binding sites with different affinities exist for both ions on each monomer. Physiological concentrations of potassium ion antagonize the binding of both divalent cations, especially affecting high-affinity calcium-binding sites.
A03659
¥37,309.00

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Overview
Specificity This product is specific to S100A1
Host Species Rabbit
Immunogen A synthesized peptide derived from human S100A1
Species Reactivity Human; Mouse
Conjugate Unconjugated

Applications
Working concentrations for specific applications should be determined by the investigators. The appropriate concentrations may be affected by secondary antibody affinity, antigen concentration, the sensitivity of the method of detection, temperature, the length of the incubations, and other factors. The suitability of this antibody for applications other than those listed below has not been determined. The following concentration ranges are recommended starting points for this product.
Application Recommended Usage
Western Blot 1:1000-1:2000
Immunohistochemistry (IHC) 1:100-1:200
Immunoprecipitation (IP) 1:20-1:50
Flow Cytometry 1:20-1:100

Properties
Form Liquid
Storage Buffer Supplied in 10mM phosphate buffered saline , pH 7.4, 150mM sodium chloride, 0.05% BSA, 0.02% sodium azide and 50% glycerol.
Storage Instructions Store at -20°C. This product is stable for 1 year upon receipt, when handled and stored as instructed.
Purification Affinity-chromatography
Isotype Rabbit IgG
Clonality Monoclonal

Examples
  • S100A1 Antibody, MAb, Rabbit
    • S100A1 Antibody, MAb, Rabbit

    Western blot analysis of S100 expression in (1) Human skeletal muscle lysate; (2) RAW 264.7 cell lysate.


Background
Target Background Weakly binds calcium but binds zinc very tightly-distinct binding sites with different affinities exist for both ions on each monomer. Physiological concentrations of potassium ion antagonize the binding of both divalent cations, especially affecting high-affinity calcium-binding sites.
Synonyms Bpb; NEF; Protein S100-A1; S100 beta; S100 calcium binding protein A1; S100A; S100B; 100beta;

For research use only. Not intended for human or animal clinical trials, therapeutic or diagnostic use.