NQO1 Antibody, mAb, Rabbit
NQO1 Antibody, MAb, Rabbit

All lanes use NQO1 Antibody, mAb, Rabbit at 1:2K dilution for 1 hour at room temperature.

NQO1 Antibody, mAb, Rabbit

Flavin-containing quinone reductase that catalyzes two-electron reduction of quinones to hydroquinones using either NADH or NADPH as electron donors. In a ping-pong kinetic mechanism, the electrons are sequentially transferred from NAD(P)H to flavin cofactor and then from reduced flavin to the quinone, bypassing the formation of semiquinone and reactive oxygen species.
A03521
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Overview
Specificity This product is specific to NQO1
Host Species Rabbit
Immunogen A synthesized peptide derived from human NQO1
Species Reactivity Human; Mouse; Rat
Conjugate Unconjugated

Applications
Working concentrations for specific applications should be determined by the investigators. The appropriate concentrations may be affected by secondary antibody affinity, antigen concentration, the sensitivity of the method of detection, temperature, the length of the incubations, and other factors. The suitability of this antibody for applications other than those listed below has not been determined. The following concentration ranges are recommended starting points for this product.
Application Recommended Usage
Western Blot 1:1000-1:2000
Immunocytochemistry/Immunofluorescence (ICC/IF) 1:50-1:200
Immunoprecipitation (IP) 1:20-1:50
Flow Cytometry 1:20-1:100

Properties
Form Liquid
Storage Buffer Supplied in 10mM phosphate buffered saline , pH 7.4, 150mM sodium chloride, 0.05% BSA, 0.02% sodium azide and 50% glycerol.
Storage Instructions Store at -20°C. This product is stable for 1 year upon receipt, when handled and stored as instructed.
Purification Affinity-chromatography
Isotype Rabbit IgG
Clonality Monoclonal

Examples
  • NQO1 Antibody, MAb, Rabbit
    • NQO1 Antibody, MAb, Rabbit

    All lanes use NQO1 Antibody, mAb, Rabbit at 1:2K dilution for 1 hour at room temperature.


Background
Target Background Flavin-containing quinone reductase that catalyzes two-electron reduction of quinones to hydroquinones using either NADH or NADPH as electron donors. In a ping-pong kinetic mechanism, the electrons are sequentially transferred from NAD(P)H to flavin cofactor and then from reduced flavin to the quinone, bypassing the formation of semiquinone and reactive oxygen species.
Synonyms Azoreductase; DT-diaphorase; DTD; QR1; NQO1; DIA4; NMOR1;

For research use only. Not intended for human or animal clinical trials, therapeutic or diagnostic use.