Caspase 3 Antibody, mAb, Rabbit
Caspase 3 Antibody, MAb, Rabbit

Western blot analysis of Caspase-3 p12 expression in HeLa cell treated with staurosporine lysate.

Caspase 3 Antibody, mAb, Rabbit

Thiol protease that acts as a major effector caspase involved in the execution phase of apoptosis.Following cleavage and activation by initiator caspases (CASP8, CASP9 and/or CASP10), mediates execution of apoptosis by catalyzing cleavage of many proteins.
A03166
¥37,309.00

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Overview
Specificity This product is specific to Caspase 3
Host Species Rabbit
Immunogen A synthesized peptide derived from human Caspase 3
Species Reactivity Human; Mouse; Rat
Conjugate Unconjugated

Applications
Working concentrations for specific applications should be determined by the investigators. The appropriate concentrations may be affected by secondary antibody affinity, antigen concentration, the sensitivity of the method of detection, temperature, the length of the incubations, and other factors. The suitability of this antibody for applications other than those listed below has not been determined. The following concentration ranges are recommended starting points for this product.
Application Recommended Usage
Western Blot 1:1000-1:2000
Immunohistochemistry (IHC) 1:100-1:200
Immunocytochemistry/Immunofluorescence (ICC/IF) 1:50-1:200
Immunohistochemistry Frozen (IHF) 1:50-1:200
Immunoprecipitation (IP) 1:20-1:50

Properties
Form Liquid
Storage Buffer Supplied in 10mM phosphate buffered saline , pH 7.4, 150mM sodium chloride, 0.05% BSA, 0.02% sodium azide and 50% glycerol.
Storage Instructions Store at -20°C. This product is stable for 1 year upon receipt, when handled and stored as instructed.
Purification Affinity-chromatography
Isotype Rabbit IgG
Clonality Monoclonal

Examples
  • Caspase 3 Antibody, MAb, Rabbit
    • Caspase 3 Antibody, MAb, Rabbit

    Western blot analysis of Caspase-3 p12 expression in HeLa cell treated with staurosporine lysate.


Background
Target Background Thiol protease that acts as a major effector caspase involved in the execution phase of apoptosis.Following cleavage and activation by initiator caspases (CASP8, CASP9 and/or CASP10), mediates execution of apoptosis by catalyzing cleavage of many proteins.
Synonyms Apopain; CASP-3; CASP3; Caspase-3 subunit p12; CPP-32; Cysteine protease CPP32; Protein Yama; SCA-1; SREBP cleavage activity 1;

For research use only. Not intended for human or animal clinical trials, therapeutic or diagnostic use.