Rb Antibody, mAb, Rabbit
Rb Antibody, MAb, Rabbit

Western blot analysis of Retinoblastoma expression in (1) Jurkat cell lysate; (2) MCF-7 cell lysate.

Rb Antibody, mAb, Rabbit

Tumor suppressor that is a key regulator of the G1/S transition of the cell cycle. The hypophosphorylated form binds transcription regulators of the E2F family, preventing transcription of E2F-responsive genes.
A03044
¥37,309.00

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Overview
Specificity This product is specific to Rb
Host Species Rabbit
Immunogen A synthesized peptide derived from human Rb
Species Reactivity Human; Mouse
Conjugate Unconjugated

Applications
Working concentrations for specific applications should be determined by the investigators. The appropriate concentrations may be affected by secondary antibody affinity, antigen concentration, the sensitivity of the method of detection, temperature, the length of the incubations, and other factors. The suitability of this antibody for applications other than those listed below has not been determined. The following concentration ranges are recommended starting points for this product.
Application Recommended Usage
Western Blot 1:1000-1:2000
Immunohistochemistry (IHC) 1:100-1:200
Immunocytochemistry/Immunofluorescence (ICC/IF) 1:50-1:200
Immunohistochemistry Frozen (IHF) 1:50-1:200
Immunoprecipitation (IP) 1:20-1:50

Properties
Form Liquid
Storage Buffer Supplied in 10mM phosphate buffered saline , pH 7.4, 150mM sodium chloride, 0.05% BSA, 0.02% sodium azide and 50% glycerol.
Storage Instructions Store at -20°C. This product is stable for 1 year upon receipt, when handled and stored as instructed.
Purification Affinity-chromatography
Isotype Rabbit IgG
Clonality Monoclonal

Examples
  • Rb Antibody, MAb, Rabbit
    • Rb Antibody, MAb, Rabbit

    Western blot analysis of Retinoblastoma expression in (1) Jurkat cell lysate; (2) MCF-7 cell lysate.


Background
Target Background Tumor suppressor that is a key regulator of the G1/S transition of the cell cycle. The hypophosphorylated form binds transcription regulators of the E2F family, preventing transcription of E2F-responsive genes.
Synonyms OSRC; RB; p105-Rb; RB1; pRb; pp110;

For research use only. Not intended for human or animal clinical trials, therapeutic or diagnostic use.