MLCK Antibody, mAb, Rabbit
MLCK Antibody, MAb, Rabbit

Western blot analysis of myosin light chain kinase expression in HUVEC cell lysate.

MLCK Antibody, mAb, Rabbit

Calcium/calmodulin-dependent myosin light chain kinase implicated in smooth muscle contraction via phosphorylation of myosin light chains (MLC). Also regulates actin-myosin interaction through a non-kinase activity. Phosphorylates PTK2B/PYK2 and myosin light-chains.
A02811
¥37,309.00

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Overview
Specificity This product is specific to MLCK
Host Species Rabbit
Immunogen A synthesized peptide derived from human MLCK
Species Reactivity Human; Mouse; Rat
Conjugate Unconjugated

Applications
Working concentrations for specific applications should be determined by the investigators. The appropriate concentrations may be affected by secondary antibody affinity, antigen concentration, the sensitivity of the method of detection, temperature, the length of the incubations, and other factors. The suitability of this antibody for applications other than those listed below has not been determined. The following concentration ranges are recommended starting points for this product.
Application Recommended Usage
Western Blot 1:1000-1:2000
Immunohistochemistry (IHC) 1:100-1:200
Immunocytochemistry/Immunofluorescence (ICC/IF) 1:50-1:200
Immunohistochemistry Frozen (IHF) 1:50-1:200
Flow Cytometry 1:20-1:100

Properties
Form Liquid
Storage Buffer Supplied in 10mM phosphate buffered saline , pH 7.4, 150mM sodium chloride, 0.05% BSA, 0.02% sodium azide and 50% glycerol.
Storage Instructions Store at -20°C. This product is stable for 1 year upon receipt, when handled and stored as instructed.
Purification Affinity-chromatography
Isotype Rabbit IgG
Clonality Monoclonal

Examples
  • MLCK Antibody, MAb, Rabbit
    • MLCK Antibody, MAb, Rabbit

    Western blot analysis of myosin light chain kinase expression in HUVEC cell lysate.


Background
Target Background Calcium/calmodulin-dependent myosin light chain kinase implicated in smooth muscle contraction via phosphorylation of myosin light chains (MLC). Also regulates actin-myosin interaction through a non-kinase activity. Phosphorylates PTK2B/PYK2 and myosin light-chains.
Synonyms KMLS; KRP; MLCK; MYLK; myosin light chain kinase, smooth muscle and non-muscle isozymes; myosin light polypeptide kinase; telokin;

For research use only. Not intended for human or animal clinical trials, therapeutic or diagnostic use.