LEF1 Antibody, mAb, Rabbit
LEF1 Antibody, MAb, Rabbit

Western blot analysis of LEF1 expression in Jurkat cell lysate.

LEF1 Antibody, mAb, Rabbit

Transcription factor that binds DNA in a sequence-specific manner.Participates in the Wnt signaling pathway.Activates transcription of target genes in the presence of CTNNB1 and EP300.PIAG antagonizes both Wnt-dependent and Wnt-independent activation by LEF1.
A02744
¥37,309.00

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Overview
Specificity This product is specific to LEF1
Host Species Rabbit
Immunogen A synthesized peptide derived from human LEF1
Species Reactivity Human; Mouse; Rat
Conjugate Unconjugated

Applications
Working concentrations for specific applications should be determined by the investigators. The appropriate concentrations may be affected by secondary antibody affinity, antigen concentration, the sensitivity of the method of detection, temperature, the length of the incubations, and other factors. The suitability of this antibody for applications other than those listed below has not been determined. The following concentration ranges are recommended starting points for this product.
Application Recommended Usage
Western Blot 1:1000-1:2000
Immunohistochemistry (IHC) 1:100-1:200
Immunocytochemistry/Immunofluorescence (ICC/IF) 1:50-1:200
Immunohistochemistry Frozen (IHF) 1:50-1:200
Immunoprecipitation (IP) 1:20-1:50
Flow Cytometry 1:20-1:100

Properties
Form Liquid
Storage Buffer Supplied in 10mM phosphate buffered saline , pH 7.4, 150mM sodium chloride, 0.05% BSA, 0.02% sodium azide and 50% glycerol.
Storage Instructions Store at -20°C. This product is stable for 1 year upon receipt, when handled and stored as instructed.
Purification Affinity-chromatography
Isotype Rabbit IgG
Clonality Monoclonal

Examples
  • LEF1 Antibody, MAb, Rabbit
    • LEF1 Antibody, MAb, Rabbit

    Western blot analysis of LEF1 expression in Jurkat cell lysate.


Background
Target Background Transcription factor that binds DNA in a sequence-specific manner.Participates in the Wnt signaling pathway.Activates transcription of target genes in the presence of CTNNB1 and EP300.PIAG antagonizes both Wnt-dependent and Wnt-independent activation by LEF1.
Synonyms LEF1_HUMAN; Lymphoid enhancer binding factor 1; TCF1 alpha; TCF10; Transcription factor T cell specific 1 alpha; TCF7L3;

For research use only. Not intended for human or animal clinical trials, therapeutic or diagnostic use.