Histone H2A.X Antibody, mAb, Rabbit
Histone H2A.X Antibody, MAb, Rabbit

All lanes use Histone H2A.X Antibody, mAb, Rabbit at 1:1K dilution for 1 hour at room temperature.

Histone H2A.X Antibody, mAb, Rabbit

Variant histone H2A which replaces conventional H2A in a subset of nucleosomes. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template.
A02663
¥37,309.00

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Overview
Specificity This product is specific to Histone H2A.X
Host Species Rabbit
Immunogen A synthesized peptide derived from human Histone H2A.X
Species Reactivity Human; Mouse; Rat
Conjugate Unconjugated

Applications
Working concentrations for specific applications should be determined by the investigators. The appropriate concentrations may be affected by secondary antibody affinity, antigen concentration, the sensitivity of the method of detection, temperature, the length of the incubations, and other factors. The suitability of this antibody for applications other than those listed below has not been determined. The following concentration ranges are recommended starting points for this product.
Application Recommended Usage
Western Blot 1:1000-1:2000
Immunohistochemistry (IHC) 1:100-1:200
Immunocytochemistry/Immunofluorescence (ICC/IF) 1:50-1:200
Immunohistochemistry Frozen (IHF) 1:50-1:200
Immunoprecipitation (IP) 1:20-1:50

Properties
Form Liquid
Storage Buffer Supplied in 10mM phosphate buffered saline , pH 7.4, 150mM sodium chloride, 0.05% BSA, 0.02% sodium azide and 50% glycerol.
Storage Instructions Store at -20°C. This product is stable for 1 year upon receipt, when handled and stored as instructed.
Purification Affinity-chromatography
Isotype Rabbit IgG
Clonality Monoclonal

Examples
  • Histone H2A.X Antibody, MAb, Rabbit
    • Histone H2A.X Antibody, MAb, Rabbit

    All lanes use Histone H2A.X Antibody, mAb, Rabbit at 1:1K dilution for 1 hour at room temperature.


Background
Target Background Variant histone H2A which replaces conventional H2A in a subset of nucleosomes. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template.
Synonyms H2A.X; H2AFX; H2a/x; HIST5-2AX; Histone H2A.X; AW228881; H2A histone family member X; Histone 2A;

For research use only. Not intended for human or animal clinical trials, therapeutic or diagnostic use.