p-PP2A alpha (Y307) Antibody, mAb, Rabbit
P-PP2A Alpha (Y307) Antibody, MAb, Rabbit

Western blot analysis of Phospho-PP2A alpha (Y307) in (1) Rat kidney lysate; (2) A431 cell lysate treated with EGF.

p-PP2A alpha (Y307) Antibody, mAb, Rabbit

PP2A is the major phosphatase for microtubule-associated proteins (MAPs). PP2A can modulate the activity of phosphorylase B kinase casein kinase 2, mitogen-stimulated S6 kinase, and MAP-2 kinase. Cooperates with SGOL2 to protect centromeric cohesin from separase-mediated cleavage in oocytes specifically during meiosis I. Can dephosphorylate SV40 large T antigen and p53/TP53. Activates RAF1 by dephosphorylating it at 'Ser-259'.
A02466
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Overview
Specificity This product is specific to p-PP2A alpha (Y307)
Host Species Rabbit
Immunogen A synthesized peptide derived from human PP2A alpha around the phosphorylation site of S307
Species Reactivity Human; Mouse; Rat
Conjugate Unconjugated

Applications
Working concentrations for specific applications should be determined by the investigators. The appropriate concentrations may be affected by secondary antibody affinity, antigen concentration, the sensitivity of the method of detection, temperature, the length of the incubations, and other factors. The suitability of this antibody for applications other than those listed below has not been determined. The following concentration ranges are recommended starting points for this product.
Application Recommended Usage
Western Blot 1:1000-1:2000
Immunohistochemistry (IHC) 1:100-1:200
Immunocytochemistry/Immunofluorescence (ICC/IF) 1:50-1:200
Immunohistochemistry Frozen (IHF) 1:50-1:200
Immunoprecipitation (IP) 1:20-1:50

Properties
Form Liquid
Storage Buffer Supplied in 10mM phosphate buffered saline , pH 7.4, 150mM sodium chloride, 0.05% BSA, 0.02% sodium azide and 50% glycerol.
Storage Instructions Store at -20°C. This product is stable for 1 year upon receipt, when handled and stored as instructed.
Purification Affinity-chromatography
Isotype Rabbit IgG
Clonality Monoclonal

Examples
  • P-PP2A Alpha (Y307) Antibody, MAb, Rabbit
    • P-PP2A Alpha (Y307) Antibody, MAb, Rabbit

    Western blot analysis of Phospho-PP2A alpha (Y307) in (1) Rat kidney lysate; (2) A431 cell lysate treated with EGF.


Background
Target Background PP2A is the major phosphatase for microtubule-associated proteins (MAPs). PP2A can modulate the activity of phosphorylase B kinase casein kinase 2, mitogen-stimulated S6 kinase, and MAP-2 kinase. Cooperates with SGOL2 to protect centromeric cohesin from separase-mediated cleavage in oocytes specifically during meiosis I. Can dephosphorylate SV40 large T antigen and p53/TP53. Activates RAF1 by dephosphorylating it at 'Ser-259'.
Synonyms eplication protein C; PP2A-alpha; Replication protein C; RP-C; PPP2CA; MGC786

For research use only. Not intended for human or animal clinical trials, therapeutic or diagnostic use.