p-LAT (Y220) Antibody, mAb, Rabbit
P-LAT (Y220) Antibody, MAb, Rabbit

Western blot analysis of Phospho-LAT (Y220) in Jurkat cell treated with CD3 lysate.

p-LAT (Y220) Antibody, mAb, Rabbit

Required for TCR (T-cell antigen receptor)- and pre-TCR-mediated signaling, both in mature T-cells and during their development. Involved in FCGR3 (low affinity immunoglobulin gamma Fc region receptor III)-mediated signaling in natural killer cells and FCER1 (high affinity immunoglobulin epsilon receptor)-mediated signaling in mast cells.
A02463
¥37,309.00

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Overview
Specificity This product is specific to p-LAT (Y220)
Host Species Rabbit
Immunogen A synthesized peptide derived from human LAT around the phosphorylation site of Y220
Species Reactivity Human; Mouse; Rat
Conjugate Unconjugated

Applications
Working concentrations for specific applications should be determined by the investigators. The appropriate concentrations may be affected by secondary antibody affinity, antigen concentration, the sensitivity of the method of detection, temperature, the length of the incubations, and other factors. The suitability of this antibody for applications other than those listed below has not been determined. The following concentration ranges are recommended starting points for this product.
Application Recommended Usage
Western Blot 1:1000-1:2000
Immunohistochemistry (IHC) 1:100-1:200
Immunocytochemistry/Immunofluorescence (ICC/IF) 1:50-1:200
Immunohistochemistry Frozen (IHF) 1:50-1:200
Immunoprecipitation (IP) 1:20-1:50

Properties
Form Liquid
Storage Buffer Supplied in 10mM phosphate buffered saline , pH 7.4, 150mM sodium chloride, 0.05% BSA, 0.02% sodium azide and 50% glycerol.
Storage Instructions Store at -20°C. This product is stable for 1 year upon receipt, when handled and stored as instructed.
Purification Affinity-chromatography
Isotype Rabbit IgG
Clonality Monoclonal

Examples
  • P-LAT (Y220) Antibody, MAb, Rabbit
    • P-LAT (Y220) Antibody, MAb, Rabbit

    Western blot analysis of Phospho-LAT (Y220) in Jurkat cell treated with CD3 lysate.


Background
Target Background Required for TCR (T-cell antigen receptor)- and pre-TCR-mediated signaling, both in mature T-cells and during their development. Involved in FCGR3 (low affinity immunoglobulin gamma Fc region receptor III)-mediated signaling in natural killer cells and FCER1 (high affinity immunoglobulin epsilon receptor)-mediated signaling in mast cells.
Synonyms 36 kDa phospho-tyrosine adaptor protein; LAT1; lat; pp36

For research use only. Not intended for human or animal clinical trials, therapeutic or diagnostic use.