IkB alpha Antibody, mAb, Rabbit
IkB Alpha Antibody, MAb, Rabbit

All lanes use IkB alpha Antibody, mAb, Rabbit at 1:2K dilution for 1 hour at room temperature.

IkB alpha Antibody, mAb, Rabbit

NFKB1 or NFKB2 is bound to REL, RELA, or RELB to form the NFKB complex. The NFKB complex is inhibited by I-kappa-B proteins (NFKBIA or NFKBIB), which inactivate NF-kappa-B by trapping it in the cytoplasm.
A02437
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Overview
Specificity This product is specific to IkB alpha
Host Species Rabbit
Immunogen A synthesized peptide derived from human IkB alpha
Species Reactivity Human; Mouse; Rat
Conjugate Unconjugated

Applications
Working concentrations for specific applications should be determined by the investigators. The appropriate concentrations may be affected by secondary antibody affinity, antigen concentration, the sensitivity of the method of detection, temperature, the length of the incubations, and other factors. The suitability of this antibody for applications other than those listed below has not been determined. The following concentration ranges are recommended starting points for this product.
Application Recommended Usage
Western Blot 1:1000-1:2000
Immunohistochemistry (IHC) 1:100-1:200
Immunocytochemistry/Immunofluorescence (ICC/IF) 1:50-1:200
Immunohistochemistry Frozen (IHF) 1:50-1:200
Immunoprecipitation (IP) 1:20-1:50

Properties
Form Liquid
Storage Buffer Supplied in 10mM phosphate buffered saline , pH 7.4, 150mM sodium chloride, 0.05% BSA, 0.02% sodium azide and 50% glycerol.
Storage Instructions Store at -20°C. This product is stable for 1 year upon receipt, when handled and stored as instructed.
Purification Affinity-chromatography
Isotype Rabbit IgG
Clonality Monoclonal

Examples
  • IkB Alpha Antibody, MAb, Rabbit
    • IkB Alpha Antibody, MAb, Rabbit

    All lanes use IkB alpha Antibody, mAb, Rabbit at 1:2K dilution for 1 hour at room temperature.


Background
Target Background NFKB1 or NFKB2 is bound to REL, RELA, or RELB to form the NFKB complex. The NFKB complex is inhibited by I-kappa-B proteins (NFKBIA or NFKBIB), which inactivate NF-kappa-B by trapping it in the cytoplasm.
Synonyms I-kappa-B-alpha; NFKBI; NFKBIA; NF-kappaB inhibitor alpha; IKBA; IkappaBalpha; MAD3; RL/IF-1;

For research use only. Not intended for human or animal clinical trials, therapeutic or diagnostic use.