beta Tubulin Antibody, mAb, Rabbit
Beta Tubulin Antibody, MAb, Rabbit

Western blot analysis of beta Tubulin expression in (1) HeLa cell lysate; (2) NIH/3T3 cell lysate; (3) C6 cell lysate.

beta Tubulin Antibody, mAb, Rabbit

Tubulin is the major constituent of microtubules, a cylinder consisting of laterally associated linear protofilaments composed of alpha- and beta-tubulin heterodimers. Microtubules grow by the addition of GTP-tubulin dimers to the microtubule end, where a stabilizing cap forms. Below the cap, tubulin dimers are in GDP-bound state, owing to GTPase activity of alpha-tubulin.
A02372
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Overview
Specificity This product is specific to beta Tubulin
Host Species Rabbit
Immunogen A synthesized peptide derived from human beta Tubulin
Species Reactivity Human; Mouse; Rat
Conjugate Unconjugated

Applications
Working concentrations for specific applications should be determined by the investigators. The appropriate concentrations may be affected by secondary antibody affinity, antigen concentration, the sensitivity of the method of detection, temperature, the length of the incubations, and other factors. The suitability of this antibody for applications other than those listed below has not been determined. The following concentration ranges are recommended starting points for this product.
Application Recommended Usage
Western Blot 1:10000-1:20000
Immunohistochemistry (IHC) 1:100-1:500
Immunoprecipitation (IP) 1:50-1:100

Properties
Form Liquid
Storage Buffer Supplied in 10mM phosphate buffered saline , pH 7.4, 150mM sodium chloride, 0.05% BSA, 0.02% sodium azide and 50% glycerol.
Storage Instructions Store at -20°C. This product is stable for 1 year upon receipt, when handled and stored as instructed.
Purification Affinity-chromatography
Isotype Rabbit IgG
Clonality Monoclonal

Examples
  • Beta Tubulin Antibody, MAb, Rabbit
    • Beta Tubulin Antibody, MAb, Rabbit

    Western blot analysis of beta Tubulin expression in (1) HeLa cell lysate; (2) NIH/3T3 cell lysate; (3) C6 cell lysate.


Background
Target Background Tubulin is the major constituent of microtubules, a cylinder consisting of laterally associated linear protofilaments composed of alpha- and beta-tubulin heterodimers. Microtubules grow by the addition of GTP-tubulin dimers to the microtubule end, where a stabilizing cap forms. Below the cap, tubulin dimers are in GDP-bound state, owing to GTPase activity of alpha-tubulin.
Synonyms Beta 4 tubulin; Beta 5 tubulin; Beta1 tubulin; Tubulin beta chain; Tubulin beta class I;

For research use only. Not intended for human or animal clinical trials, therapeutic or diagnostic use.