Protein Isolation and Analysis » Protein A Antibody [Biotin], mAb, Mouse

Protein A Antibody [Biotin], mAb, Mouse

GenScript Protein A Antibody [Biotin], mAb, Mouse detects natural Protein A, variety of recombinant Protein A variants and MabSelect SuReTM ligand.
A01779
¥19,187.00

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Overview
Specificity GenScript Protein A Antibody [Biotin], mAb, Mouse detects natural Protein A, variety of recombinant Protein A variants and MabSelect SuReTM ligand.
Host Species Mouse
Immunogen purified recombinant Protein A
Conjugate Biotin

Applications
Working concentrations for specific applications should be empirically determined by the investigator. The appropriate concentrations may be affected by secondary antibody affinity, antigen concentration, the sensitivity of the detection methods, temperature, the length of the incubations, and other factors. The suitability of this antibody for applications other than those listed below has not been determined. The following concentration ranges are recommended starting points for this product.
Application Recommended Usage
Sandwich ELISA Capture with A01778 and detected by A01779
Capture: 1-10 µg/ml
Detection: 0.05-0.2 μg/ml
Western Blot 0.5 -1 µg/ml

Properties
Form lyophilized
Storage Buffer lyophilized with PBS, pH 7.4, containing 10 mg/mL BSA and 0.02% sodium azide.
Reconstitution Reconstitute the lyophilized powder with deionized water (or equivalent) to an final concentration of 0.5 mg/mL.
Storage Instructions The lyophilized product remains stable for 1 year at -20℃ from the date of receipt. Upon reconstitution, it can be stored for 2-3 weeks at 2-8℃ or for 12 months at -20℃ or below. Avoid repeated freezing and thawing cycles.
Purification Protein A affinity column
Isotype Mouse IgG1,κ
Clonality Monoclonal

Background
Target Background Protein A, a bacterial cell wall protein isolated from Staphylococcus aureus, binds to mammalian IgGs mainly through Fc regions. The Protein A is most commonly bound to a chromatography resin, used for production of antibody drugs or reagents. Protein A antibody can be used for quantification of Protein A in sample foods or for evaluation of Protein A resin. This Protein A mAb is advantageous due to its IgG1 isotype, for mouse IgG1 has weak binding capacity to Protein A.GenScript Protein A Antibody [Biotin], mAb, Mouse is Protein A Antibody conjugated with biotin. The conjugated antibody is suitable for detecting the expression level of Protein A.
Synonyms Protein A Antibody [Biotin], mAb, Mouse;

Limited Use License and Use Restrictions
Limited Use License and Use Restrictions This Product is provided under a limited, non-exclusive, non-transferable, and non-sublicensable end-user license strictly limited to the permitted uses set forth in GenScript’s official documentation, quotations, or written agreements. Any use beyond such scope requires GenScript’s prior written authorization or license.
Except as expressly authorized in writing by GenScript, Customer shall not, and shall not permit, procure, enable, or assist any third party to: (a) commercialize or otherwise exploit the Product, including by reselling, distributing, transferring, sublicensing, or otherwise making the Product (including any product incorporating the Product) available to any third party; (b) copy, reproduce, clone, reverse clone, sequence, analyze, characterize, deconstruct, reconstruct, reverse engineer, or otherwise attempt to discover the composition, sequence, structure, binding characteristics, epitope recognition characteristics, or other proprietary features of the Product; (c) modify, derivatize, humanize, optimize, mutate, design around, or generate any variants, derivatives, fragments, substitutes, or functional equivalents of the Product; or (d) use the Product, any information derived therefrom, or any computational, AI-enabled, or sequence-based design method to develop, manufacture, offer for sale, sell, or commercialize any product or service competitive with the Product or GenScript’s products or services.
All intellectual property rights and all rights not expressly granted herein are reserved by GenScript. No rights are granted by implication, estoppel, or otherwise.

For laboratory research use only. Direct human use, including taking orally and injection and clinical use are forbidden.