gRNAs
The following Lars2 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Lars2 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.
| CloneID | OMu43272 | |
| Clone ID Related Accession (Same CDS sequence) | NM_001348168.1 , XM_017313062.1 | |
| Accession Version | NM_001348168.1 Latest version! | Documents for ORF clone product in default vector |
| Sequence Information | ORF Nucleotide Sequence (Length: 1965bp) Protein sequence SNP |
|
| Vector | pcDNA3.1-C-(k)DYK or customized vector |  User Manual |
| Clone information | Clone Map | MSDS |
| Tag on pcDNA3.1+/C-(K)DYK | C terminal DYKDDDDK tags | |
| ORF Insert Method | CloneEZ™ Seamless cloning technology | |
| Insert Structure | linear | |
| Update Date | 2019-09-09 | |
| Organism | Mus musculus(house mouse) | |
| Product | probable leucine--tRNA ligase, mitochondrial isoform 2 | |
| Comment | Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from BY282131.1, BC080303.1, AC125254.9 and BY630405.1. Transcript Variant: This variant (3) differs in the 5' UTR, has multiple coding region differences, and initiates translation at an alternate start site compared to variant 1. It encodes isoform 2 which has a shorter N-terminus compared to isoform 1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. ##Evidence-Data-START## RNAseq introns :: single sample supports all introns SAMN00849380, SAMN00849381 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## gene product(s) localized to mito. :: reported by MitoCarta ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end. | |
1 | ATGGCCATGC AGGATGCGTT GGCAGACCTC CCAGAGTGGT ACGGAATCAA AGGCATGCAG |
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
| RefSeq | NP_001335097.1 |
| CDS | 1145..3109 |
| Translation |
Target ORF information:
Target ORF information:
|
 NM_001348168.1 |
1 | ATGGCCATGC AGGATGCGTT GGCAGACCTC CCAGAGTGGT ACGGAATCAA AGGCATGCAG |
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
| CloneID | OMu43272 | |
| Clone ID Related Accession (Same CDS sequence) | NM_001348168.1 , XM_017313062.1 | |
| Accession Version | XM_017313062.1 Latest version! | Documents for ORF clone product in default vector |
| Sequence Information | ORF Nucleotide Sequence (Length: 1965bp) Protein sequence SNP |
|
| Vector | pcDNA3.1-C-(k)DYK or customized vector | User Manual |
| Clone information | Clone Map | MSDS |
| Tag on pcDNA3.1+/C-(K)DYK | C terminal DYKDDDDK tags | |
| ORF Insert Method | CloneEZ™ Seamless cloning technology | |
| Insert Structure | linear | |
| Update Date | 2016-06-21 | |
| Organism | Mus musculus(house mouse) | |
| Product | probable leucine--tRNA ligase, mitochondrial isoform X1 | |
| Comment | Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000075.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## | |
1 | ATGGCCATGC AGGATGCGTT GGCAGACCTC CCAGAGTGGT ACGGAATCAA AGGCATGCAG |
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
| RefSeq | XP_017168551.1 |
| CDS | 1156..3120 |
| Translation |
Target ORF information:
Target ORF information:
|
XM_017313062.1 |
1 | ATGGCCATGC AGGATGCGTT GGCAGACCTC CCAGAGTGGT ACGGAATCAA AGGCATGCAG |
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
| CloneID | OMu13623 | |
| Clone ID Related Accession (Same CDS sequence) | NM_001348167.1 , NM_153168.3 | |
| Accession Version | NM_001348167.1 Latest version! | Documents for ORF clone product in default vector |
| Sequence Information | ORF Nucleotide Sequence (Length: 2709bp) Protein sequence SNP |
|
| Vector | pcDNA3.1-C-(k)DYK or customized vector | User Manual |
| Clone information | Clone Map | MSDS |
| Tag on pcDNA3.1+/C-(K)DYK | C terminal DYKDDDDK tags | |
| ORF Insert Method | CloneEZ™ Seamless cloning technology | |
| Insert Structure | linear | |
| Update Date | 2019-10-24 | |
| Organism | Mus musculus(house mouse) | |
| Product | probable leucine--tRNA ligase, mitochondrial isoform 1 | |
| Comment | Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AC164123.4, BC080303.1 and BY630405.1. Transcript Variant: This variant (2) uses an alternate splice site in the 5' UTR compared to variant 1. Both variants 1 and 2 encode the same isoform (1). Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. ##Evidence-Data-START## RNAseq introns :: single sample supports all introns SAMN01164131, SAMN01164132 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## gene product(s) localized to mito. :: reported by MitoCarta ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end. | |
1 | ATGGCATCCA CTTGTCAGAG GCTGAGTTTC TATGTCTCTC CTCTGAAAAG GCAGCTAGTT |
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
| RefSeq | NP_001335096.1 |
| CDS | 532..3240 |
| Translation |
Target ORF information:
Target ORF information:
|
NM_001348167.1 |
1 | ATGGCATCCA CTTGTCAGAG GCTGAGTTTC TATGTCTCTC CTCTGAAAAG GCAGCTAGTT |
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
| CloneID | OMu13623 | |
| Clone ID Related Accession (Same CDS sequence) | NM_001348167.1 , NM_153168.3 | |
| Accession Version | NM_153168.3 Latest version! | Documents for ORF clone product in default vector |
| Sequence Information | ORF Nucleotide Sequence (Length: 2709bp) Protein sequence SNP |
|
| Vector | pcDNA3.1-C-(k)DYK or customized vector | User Manual |
| Clone information | Clone Map | MSDS |
| Tag on pcDNA3.1+/C-(K)DYK | C terminal DYKDDDDK tags | |
| ORF Insert Method | CloneEZ™ Seamless cloning technology | |
| Insert Structure | linear | |
| Update Date | 2019-09-09 | |
| Organism | Mus musculus(house mouse) | |
| Product | probable leucine--tRNA ligase, mitochondrial isoform 1 | |
| Comment | Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from BY282131.1, BC080303.1 and BY630405.1. On Feb 8, 2017 this sequence version replaced NM_153168.2. Transcript Variant: This variant (1) encodes the longer isoform (1). Both variants 1 and 2 encode the same isoform (1). ##Evidence-Data-START## RNAseq introns :: single sample supports all introns SAMN00849374, SAMN00849375 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## gene product(s) localized to mito. :: reported by MitoCarta ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end. | |
1 | ATGGCATCCA CTTGTCAGAG GCTGAGTTTC TATGTCTCTC CTCTGAAAAG GCAGCTAGTT |
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
| RefSeq | NP_694808.1 |
| CDS | 200..2908 |
| Translation |
Target ORF information:
Target ORF information:
|
NM_153168.3 |
1 | ATGGCATCCA CTTGTCAGAG GCTGAGTTTC TATGTCTCTC CTCTGAAAAG GCAGCTAGTT |
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
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