TRIP12 gene cDNA ORF clone, Gallus gallus(chicken)

mRNA	Protein	Name
XM_015276964.1	XP_015132450.1	E3 ubiquitin-protein ligase TRIP12 isoform X1
XM_015276971.1	XP_015132457.1	E3 ubiquitin-protein ligase TRIP12 isoform X5
XM_015276968.2	XP_015132454.1	E3 ubiquitin-protein ligase TRIP12 isoform X1
XM_025153691.1	XP_025009459.1	E3 ubiquitin-protein ligase TRIP12 isoform X6
XM_015276967.1	XP_015132453.1	E3 ubiquitin-protein ligase TRIP12 isoform X1
XM_015276968.1	XP_015132454.1	E3 ubiquitin-protein ligase TRIP12 isoform X1
XM_004937064.3	XP_004937121.1	E3 ubiquitin-protein ligase TRIP12 isoform X1
XM_015276964.2	XP_015132450.1	E3 ubiquitin-protein ligase TRIP12 isoform X1
XM_015276966.2	XP_015132452.1	E3 ubiquitin-protein ligase TRIP12 isoform X1
XM_004937064.2	XP_004937121.1	E3 ubiquitin-protein ligase TRIP12 isoform X1
XM_015276966.1	XP_015132452.1	E3 ubiquitin-protein ligase TRIP12 isoform X1
XM_015276969.1	XP_015132455.1	E3 ubiquitin-protein ligase TRIP12 isoform X3
XM_015276967.2	XP_015132453.1	E3 ubiquitin-protein ligase TRIP12 isoform X1
XM_004937065.3	XP_004937122.1	E3 ubiquitin-protein ligase TRIP12 isoform X2
XM_015276965.1	XP_015132451.1	E3 ubiquitin-protein ligase TRIP12 isoform X1
XM_004937065.2	XP_004937122.1	E3 ubiquitin-protein ligase TRIP12 isoform X2
XM_015276972.1	XP_015132458.1	E3 ubiquitin-protein ligase TRIP12 isoform X6
XM_015276970.2	XP_015132456.1	E3 ubiquitin-protein ligase TRIP12 isoform X4
XM_015276971.2	XP_015132457.1	E3 ubiquitin-protein ligase TRIP12 isoform X5
XM_015276970.1	XP_015132456.1	E3 ubiquitin-protein ligase TRIP12 isoform X4
XM_015276969.2	XP_015132455.1	E3 ubiquitin-protein ligase TRIP12 isoform X3
XM_025153693.1	XP_025009461.1	E3 ubiquitin-protein ligase TRIP12 isoform X7
XM_015276972.2	XP_015132458.1	E3 ubiquitin-protein ligase TRIP12 isoform X8

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OGa152842	XM_025153693.1 Latest version!	Gallus gallus thyroid hormone receptor interactor 12 (TRIP12), transcript variant X11, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OGa39718	XM_015276972.2 Latest version!	Gallus gallus thyroid hormone receptor interactor 12 (TRIP12), transcript variant X12, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$1021.30 $1459.00
OGa152841	XM_025153691.1 Latest version!	Gallus gallus thyroid hormone receptor interactor 12 (TRIP12), transcript variant X10, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1091.30 $1559.00
OGa39716	XM_015276969.2 Latest version!	Gallus gallus thyroid hormone receptor interactor 12 (TRIP12), transcript variant X7, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1161.30 $1659.00
OGa39717	XM_015276970.2 Latest version!	Gallus gallus thyroid hormone receptor interactor 12 (TRIP12), transcript variant X8, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1161.30 $1659.00
OGa12502	XM_015276964.2 Latest version!	Gallus gallus thyroid hormone receptor interactor 12 (TRIP12), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	30	$1161.30 $1659.00
OGa12502	XM_015276967.2 Latest version!	Gallus gallus thyroid hormone receptor interactor 12 (TRIP12), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	30	$1161.30 $1659.00
OGa12502	XM_015276968.2 Latest version!	Gallus gallus thyroid hormone receptor interactor 12 (TRIP12), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	30	$1161.30 $1659.00
OGa12502	XM_004937064.3 Latest version!	Gallus gallus thyroid hormone receptor interactor 12 (TRIP12), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	30	$1161.30 $1659.00
OGa12502	XM_015276966.2 Latest version!	Gallus gallus thyroid hormone receptor interactor 12 (TRIP12), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	30	$1161.30 $1659.00
OGa12502	XM_015276965.1 Latest version!	Gallus gallus thyroid hormone receptor interactor 12 (TRIP12), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	30	$1161.30 $1659.00
OGa12503	XM_004937065.3 Latest version!	Gallus gallus thyroid hormone receptor interactor 12 (TRIP12), transcript variant X6, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	30	$1161.30 $1659.00
OGa12504	XM_015276971.2 Latest version!	Gallus gallus thyroid hormone receptor interactor 12 (TRIP12), transcript variant X9, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	30	$1161.30 $1659.00
View Old Accession Versions >>
OGa12502	XM_015276968.1	Gallus gallus thyroid hormone receptor interactor 12 (TRIP12), transcript variant X6, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	30	$1161.30 $1659.00
OGa12502	XM_015276964.1	Gallus gallus thyroid hormone receptor interactor 12 (TRIP12), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	30	$1161.30 $1659.00
OGa12502	XM_004937064.2	Gallus gallus thyroid hormone receptor interactor 12 (TRIP12), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	30	$1161.30 $1659.00
OGa12502	XM_015276966.1	Gallus gallus thyroid hormone receptor interactor 12 (TRIP12), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	30	$1161.30 $1659.00
OGa12502	XM_015276967.1	Gallus gallus thyroid hormone receptor interactor 12 (TRIP12), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	30	$1161.30 $1659.00
OGa12503	XM_004937065.2	Gallus gallus thyroid hormone receptor interactor 12 (TRIP12), transcript variant X7, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	30	$1161.30 $1659.00
OGa12504	XM_015276971.1	Gallus gallus thyroid hormone receptor interactor 12 (TRIP12), transcript variant X10, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	30	$1161.30 $1659.00
OGa39717	XM_015276970.1	Gallus gallus thyroid hormone receptor interactor 12 (TRIP12), transcript variant X9, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1161.30 $1659.00
OGa39716	XM_015276969.1	Gallus gallus thyroid hormone receptor interactor 12 (TRIP12), transcript variant X8, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1161.30 $1659.00
OGa39718	XM_015276972.1	Gallus gallus thyroid hormone receptor interactor 12 (TRIP12), transcript variant X11, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$1021.30 $1459.00
Hide Old Accession Versions >>

TRIP12 ( XM_025153693.1 ) cDNA ORF clone, Gallus gallus(chicken) -> XP_025009461.1 Gallus gallus thyroid hormone receptor interactor 12 (TRIP12), transcript variant X11, mRNA.

Price & Availability↑

CloneID	OGa152842
Clone ID Related Accession (Same CDS sequence)	XM_025153693.1
Accession Version	XM_025153693.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5976bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-16
Organism	Gallus gallus(chicken)
Product	E3 ubiquitin-protein ligase TRIP12 isoform X7
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006096.5) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Gallus gallus Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCCAACC GGCCTAATAA CAATCCAGGG GGGTCACTGC GACGTTCACA GAGGAACACT 
GCTGGGGCCC AGCCACAAGA CGACGCAGCA GGAGGAAGAG GCTGTAGTTC ATCTGCTGTT 
TTAATACCAC AAGAAGATTC AGAAAGAGTC AGTACTTCAG AAAAGCAAAA AACGGGGCAA 
GTGCCTAAGA AAGACAATTC TCGAGGAGTT AAACGCAGTG CTAGTCCAGA TTACAGCAGG 
ACCAATTCTC CTAGCTCTGC TAAAAAACCT AAAGCACTTC AACACGCCGA AACTTCCTCG 
GAAAGTAACA AGCCACATAC TAAATCCAAG AGAAGACACT TAGACCAAGA ACAGCAGAAG 
TCTACACAAT TGCCATCAAC AAGCAAGGCT CACACCAGAA AGGGTGGAGC TGCTGGTAGC 
TCCCGAAGTC AGAAAAGGAA AAGGACAGAG AGTACGTCTT GTATAAAGAG TCGTTCAGCA 
GTTGAATCAA CTGGAGCTGA AGAGAAGTCA GCAAAATCCT CCAAGCTGGC TTCAAAATCG 
GTGACCTCAG CCAAAGCTGG GTGTAGCACC ATCACTGATT CTTCTTCTTC AGCTTCTACA 
TCCTCTTCCT CTTCTGCTGT TGCCTCTGCT TCTTCCACTG TTCCTCAGGG TGCCAGAGTG 
AAACAGGGTA AGGACCAGAA TAAGGCTAGA CGTTCACGTT CTGCGTCCAG CCCTAGTCCA 
AGAAGGAGCA GCAGGGACAA AGAACAGAGC AAAACAGGTG GCTCTTCAAA GTTTGACTGG 
GCTGCTCGAT TCAGTCCAAA AGTTAGTCTC CCTAAAACAA AACTGTCTCT ACCAGGCTCT 
TCTAAGTCAG AGACCTCAAA ACCTGGACCT TCAGGACTAC AGGCTAAGCT AGCAAGCCTA 
AGAAAATCCA CAAAGAAGCG CAGTGAATCA CCACCTGCTG AGCTCCCCAG TTTACGGCGG 
AGCACACGGC AAAAGACCAC GGGCTCCTGT GCGAGTACCA GTCGGCGGGG CTCTGGCCTG 
GGCAAAAGAG GAGCAGCTGA AGCTCGCCGA CAGGAGAAGA TGGCTGATCC TGACAACAAC 
CAGGATGGAG TTAACTCTTC AGCTGCACGT ACAGATGAGA CTCCCCAGGG AGCTGCAGCT 
TCTAGCTCTG TTGCTGGGGC TGTAGGTATG ACAACATCTG GAGAAAGTGA GTCAGATGAC 
TCTGAGATGG GAAGACTACA AGGTTCTAAA GCCCAGCAGC TTTTACAAGG TCTCCAAGCC 
ACTGATGAAA GTCAGCAACT ACAGGCAGTT ATTGAGATGT GCCAGCTGTT GGTCATGGGA 
AATGAAGAAA CACTGGGAGG ATTTCCAGTC AAGAGTGTTG TGCCAGCTTT GATAACATTG 
CTGCAGATGG AGCATAACTT TGACATCATG AACCATGCTT GTCGAGCCTT AACATACATG 
ATGGAAGCAC TTCCCAGATC ATCTGCTGTA GTGGTAGATG CAATTCCTGT CTTCTTGGAA 
AAGCTGCAAG TTATTCAGTG CATTGATGTG GCAGAGCAGG CACTGACAGC CCTAGAGATG 
CTATCACGCA GGCATAGTAA AGCCATTCTG CAGGCAGGTG GGTTGGCAGA CTGCTTGCTG 
TATCTGGAAT TCTTCAGTAT AAATGCGCAG AGGAATGCAC TAGCTATTGC TGCCAATTGC 
TGCCAGAGTA TAACACCTGA TGAGTTTCAC TTTGTGGCAG ACTCTTTGCC ACTGCTTACA 
CAAAGGTTAA CCCATCAGGA CAAGAAGTCT GTTGAAAGCA CTTGTCTCTG TTTTGCACGG 
CTGGTAGACA ATTTCCAGCA TGAGGAGAAC TTGCTGCAGC AGGTTGCTTC CAAGGACTTG 
TTAACAAACA TCCAGCAACT CTTGGTAGTG ACACCTCCTA TCCTGAGCTC AGGAATGTTC 
ATCATGGTGG TGCGCATGTT TTCCTTAATG TGTTCCAATT GTCCAACGCT TGCAGTCCAA 
CTTATGAAGC AAAACATTGC AGAAACACTT CACTTTCTCC TTTGTGGAGC CTCAAATGGG 
AGTTGTCAAG AACAAATTGA CCTTGTTCCA CGAAGTCCTC AAGAACTCTA TGAGCTTACT 
TCTCTTATCT GTGAACTGAT GCCTTGTCTG CCAAAAGAGG GGATATTTGC TGTTGATACT 
ATGCTAAAGA AAGGAAATGC ACAAAATACA GATGGTGCGA TATGGCAATG GCGAGATGAC 
AGGGGTCTCT GGCATCCCTA TAACAGGATT GATAGTCGAA TAATAGAGGC AGCTCATCAG 
GTTGGTGAGG ATGAGATAAG CCTGTCGACA CTTGGACGTG TCTATACTAT TGATTTTAAT 
TCTATGCAGC AAATAAATGA GGATACTGGA ACAGCACGTG CCATTCAGCG GAAACCAAAC 
CCTTTAGCCA ATACAAACAC TAGTGGACAT TCAGAATTGA AGAAGGATGA TGCTCGAGCA 
CAGCTAATGA AAGAAGACCC AGAATTGGCA AAATCCTTTA TCAAAACACT GTTTGGTGTT 
CTTTATGAAG TGTATAGTTC TTCAGCTGGA CCTGCTGTTA GACACAAGTG CCTTAGAGCA 
ATACTTAGAA TAATTTATTT TGCTGATGCT GAACTCCTGA AGGATGTGCT GAAAAACCAT 
GCTGTGTCAA GTCACATTGC CTCCATGCTG TCAAGTCAAG ACCTTAAGAT AGTAGTTGGA 
GCCCTGCAGA TGGCAGAAAT CTTAATGCAG AAGCTACCTG ACATTTTTAG TGTTTACTTC 
AGAAGAGAAG GAGTGATGCA TCAAGTGAAA AACTTAGCGG AGTCTGAGGC TTTGCTAACG 
AGCCCACCAA AAGTATGCAC TAATGGATCA GGAACACTGG GTACCACTAC AACTATAAGT 
ACTGGAACAG CCACTGCTGC CACTAATGCA GCTGCAGATT TGGGCTCTCC CAGCTTACAA 
CACAGCCGGG AGGATTCTTT AGATCTGAGC CCACAGGGAC GACTGAGTGA TGTTCTGAAG 
AGAAAAAGAC TGCCAAAACG AGGGCCAAGG AGACCAAAAT ATTCTCCCCC AAGAGATGAT 
GACAAAGTAG ACAATCAAGC AAAAAGCCCC ACGACTACTC AGTCTCCTAA ATCTTCTTTC 
TTAGCAAGTT TAAATCCCAA AACATGGGGA AGATTAAGTA CACAGTCTAA CAGTAATAAC 
ATTGAACCAG CAAGAACAGC AGGTGTAAGT GGTCTTGCAA GAGCTGCTTC TAAGGATACA 
ATTTCCAATA ACAGAGAAAA AATTAAGGGT TGGATTAAGG AGCAAGCCCA TAAATTTGTA 
GACCGTTATT TTAGTTCGGA AAACATGGAT GGAAGCAATC CTGCACTAAA TGTATTACAG 
AGACTTTGCA CTGCAACTGA ACAACTTAAC CTCCAGGTGG ATGGTGGAAC AGAGTGCCTT 
GTAGAAATCC GTAGCATTGT CTCGGAGTCT GACGTCTCCT CATTTGAAAT CCAGCATAGT 
GGATTTGTGA AACAACTGCT GCTTTATTTG ACATCTAAAA GTGAGAAAGA TGCTGTAAGC 
AGGGATATCA GATTGAAAAG ATTTCTTCAT GTATTTTTCT CCTCTCCACT TCCTGGAGAA 
GAACCCCTTG GAAGATTAGA GCCATTAGAA AATGCACCTT TGTTGGCATT AGTCCATAAA 
ATGAACAATT GCCTCAGTCA GATGGAGCAG TTTCCAGTGA AAGTGCATGA CTTCCCCAGT 
GGAAATGGAA CAGGGAGCAG TTTTTCTCTT AACAGAGGAT CCCAAGCTTT AAAATTCTTC 
AATACGCATC AGTTAAAATG CCAGCTGCAA AGACATCCAG ACTGTGCTAA TGTGAAACAG 
TGGAAGGGTG GACCTGTGAA GATTGATCCT CTGGCTTTGG TTCAAGCCAT TGAAAGATAT 
CTTGTTGTTA GAGGTTATGG AAGAGTTAGA GAAGATGATG AGGATAGTGA TGATGATGGG 
TCAGATGAAG AAATAGATGA ATCCTTGGCT GCTCAATTCT TAAATTCAGG GAATGTGAGA 
CATAGATTGC AGTTTTACAT TGGGGATCAC TTGCTACCAT ATAATATGAC TGTATATCAA 
GCAGTTAAGC TGTACAGTTT GCAAGCTGAG GAGGAGAGGG AATCGACTGA TGATGAAAGC 
AATCCATTAG GAAGAGCTGG GATTTGGACA AAAACACATA CCATTTGGTA CAAACCTGTA 
CGGGAGGATG AAGATGGCAA CAAAGACTGT GTTGGTGGTA AAAGAGGAAG AGCTCAAACT 
GCTCCCACAA AAACCTCACC TAGGAATTCT AAAAAGCATG ATGAGTTGTG GCACGATGGT 
GTATGCCCTT CAGTATTGAA TCCTTTAGAA GTTTACCTCA TATCTACTCC TCCTGAAAAC 
ATAACCTTTG AAGATCCTTC ATTAGATGTT ATTCTTCTTT TGAGAGTTTT ACATGCTATC 
AGTCGATACT GGTATTATTT GTATGATAAT GCAATATGCA AGGAGATTAT TCCAACTTCG 
GAATTTATCA ATAGTAAACT GACAGCAAAA GCAAACAGAC AGCTTCAAGA TCCACTGGTA 
ATTATGACAG GAAACATACC GACTTGGCTG ACAGAGCTTG GAAAAACATG CCCATTTTTC 
TTTCCATTTG ATACCCGGCA AATGCTGTTT TATGTAACTG CTTTTGATCG TGATCGAGCC 
ATGCAAAGAC TACTGGATAC TAATCCAGAA ATCAATCAAT CAGATTCTCA GGATAGCAGA 
GTGGCACCAC GACTGGACAG GAAAAAACGC ACTGTGAACA GAGATGAGCT GTTGAAACAG 
GCAGAATCTG TGATGCAGGA TCTAGGCAGT TCAAGAGCCA TGTTGGAAAT CCAGTATGAG 
AATGAAGTTG GCACAGGCCT GGGCCCTACA CTAGAGTTCT ATGCACTTGT ATCTCAGGAA 
CTACAGAGAG CAGACTTGGG CCTTTGGAGG GGAGAAGAAG TAACTTTAGC CAATCCAAAA 
GGAAGCCAAG AAGGTACCAA GTATATTCAT AATCTCCAAG GCCTTTTTGC ACTTCCTTTT 
GGTAGAACAG CCAAGCCTGC TCACATTGCA AAAGTTAAAA TGAAGTTCCG CTTTCTGGGA 
AAACTAATGG CTAAGGCAAT CATGGATTTT AGACTGGTGG ATCTTCCTCT TGGACTTCCT 
TTTTATAAAT GGATGTTACG CCAGGAAACT TCTTTGACGT CACATGACTT GTTCAGTATT 
GATCCAGTAG TAGCCAAATC AATATATCAC CTTGAAGATA TTGTAAGACA AAAGAAAAGA 
CTTGAGCAGG ACAAAACACA GACCAAAGAA AGTCTACAGT ATGCATTGGA AACCCTGACT 
ATGAATGGCT GCTCAGTGGA GGATCTAGGG CTGGATTTCA CACTTCCTGG ATTTCCTAAC 
ATCGAGCTGA AAAAAGGGGG AAAAGATACA CCAGTCACCA TCCACAACTT GGAGGAGTAC 
CTCAGATTGG TTATATTCTG GGCACTTAAC GAAGGTGTTG CCAGACAGTT TGATTCTTTC 
AGAGATGGAT TTGAATCAGT CTTCCCCCTC AGTCATCTTC AATACTTTTA TCCTGAGGAG 
TTGGAGCAAC TCTTATGTGG CAGCAAAACA GACACATGGG ATGCCAAGAC CTTAATGGAA 
TGTTGCAGGC CAGATCACGG TTATACACAT GACAGTCGAG CAGTGAAGTA TCTCTTTGAA 
ATTCTCAGTA GTTTTGATAG TGAGCAGCAG AGACTGTTTC TTCAGTTTGT GACCGGTAGT 
CCCAGGCTGC CAGTAGGAGG CTTTCGAAGT TTGAATCCTC CATTGACAAT TGTACGGAAG 
ACATTTGAGT CTACAGAGAA TCCAGATGAC TTCTTGCCCT CAGTAATGAC TTGTGTGAAC 
TATCTCAAAT TGCCGGACTA TTCAACTATT GAGATAATGC GTGAAAAACT CTTGATAGCT 
GCAAGAGAAG GGCAGCAGTC ATTCCATCTT TCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_025009461.1
CDS	138..6113
Translation	MSNRPNNNPGGSLRRSQRNTAGAQPQDDAAGGRGCSSSAVLIPQEDSERVSTSEKQKTGQVPKKDNSRGVKRSASPDYSRTNSPSSAKKPKALQHAETSSESNKPHTKSKRRHLDQEQQKSTQLPSTSKAHTRKGGAAGSSRSQKRKRTESTSCIKSRSAVESTGAEEKSAKSSKLASKSVTSAKAGCSTITDSSSSASTSSSSSAVASASSTVPQGARVKQGKDQNKARRSRSASSPSPRRSSRDKEQSKTGGSSKFDWAARFSPKVSLPKTKLSLPGSSKSETSKPGPSGLQAKLASLRKSTKKRSESPPAELPSLRRSTRQKTTGSCASTSRRGSGLGKRGAAEARRQEKMADPDNNQDGVNSSAARTDETPQGAAASSSVAGAVGMTTSGESESDDSEMGRLQGSKAQQLLQGLQATDESQQLQAVIEMCQLLVMGNEETLGGFPVKSVVPALITLLQMEHNFDIMNHACRALTYMMEALPRSSAVVVDAIPVFLEKLQVIQCIDVAEQALTALEMLSRRHSKAILQAGGLADCLLYLEFFSINAQRNALAIAANCCQSITPDEFHFVADSLPLLTQRLTHQDKKSVESTCLCFARLVDNFQHEENLLQQVASKDLLTNIQQLLVVTPPILSSGMFIMVVRMFSLMCSNCPTLAVQLMKQNIAETLHFLLCGASNGSCQEQIDLVPRSPQELYELTSLICELMPCLPKEGIFAVDTMLKKGNAQNTDGAIWQWRDDRGLWHPYNRIDSRIIEAAHQVGEDEISLSTLGRVYTIDFNSMQQINEDTGTARAIQRKPNPLANTNTSGHSELKKDDARAQLMKEDPELAKSFIKTLFGVLYEVYSSSAGPAVRHKCLRAILRIIYFADAELLKDVLKNHAVSSHIASMLSSQDLKIVVGALQMAEILMQKLPDIFSVYFRREGVMHQVKNLAESEALLTSPPKVCTNGSGTLGTTTTISTGTATAATNAAADLGSPSLQHSREDSLDLSPQGRLSDVLKRKRLPKRGPRRPKYSPPRDDDKVDNQAKSPTTTQSPKSSFLASLNPKTWGRLSTQSNSNNIEPARTAGVSGLARAASKDTISNNREKIKGWIKEQAHKFVDRYFSSENMDGSNPALNVLQRLCTATEQLNLQVDGGTECLVEIRSIVSESDVSSFEIQHSGFVKQLLLYLTSKSEKDAVSRDIRLKRFLHVFFSSPLPGEEPLGRLEPLENAPLLALVHKMNNCLSQMEQFPVKVHDFPSGNGTGSSFSLNRGSQALKFFNTHQLKCQLQRHPDCANVKQWKGGPVKIDPLALVQAIERYLVVRGYGRVREDDEDSDDDGSDEEIDESLAAQFLNSGNVRHRLQFYIGDHLLPYNMTVYQAVKLYSLQAEEERESTDDESNPLGRAGIWTKTHTIWYKPVREDEDGNKDCVGGKRGRAQTAPTKTSPRNSKKHDELWHDGVCPSVLNPLEVYLISTPPENITFEDPSLDVILLLRVLHAISRYWYYLYDNAICKEIIPTSEFINSKLTAKANRQLQDPLVIMTGNIPTWLTELGKTCPFFFPFDTRQMLFYVTAFDRDRAMQRLLDTNPEINQSDSQDSRVAPRLDRKKRTVNRDELLKQAESVMQDLGSSRAMLEIQYENEVGTGLGPTLEFYALVSQELQRADLGLWRGEEVTLANPKGSQEGTKYIHNLQGLFALPFGRTAKPAHIAKVKMKFRFLGKLMAKAIMDFRLVDLPLGLPFYKWMLRQETSLTSHDLFSIDPVVAKSIYHLEDIVRQKKRLEQDKTQTKESLQYALETLTMNGCSVEDLGLDFTLPGFPNIELKKGGKDTPVTIHNLEEYLRLVIFWALNEGVARQFDSFRDGFESVFPLSHLQYFYPEELEQLLCGSKTDTWDAKTLMECCRPDHGYTHDSRAVKYLFEILSSFDSEQQRLFLQFVTGSPRLPVGGFRSLNPPLTIVRKTFESTENPDDFLPSVMTCVNYLKLPDYSTIEIMREKLLIAAREGQQSFHLS

Target ORF information:

RefSeq Version	XM_025153693.1
Organism	Gallus gallus(chicken)
Definition	Gallus gallus thyroid hormone receptor interactor 12 (TRIP12), transcript variant X11, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_025153693.1

ORF Insert Sequence:

ATGTCCAACC GGCCTAATAA CAATCCAGGG GGGTCACTGC GACGTTCACA GAGGAACACT 
GCTGGGGCCC AGCCACAAGA CGACGCAGCA GGAGGAAGAG GCTGTAGTTC ATCTGCTGTT 
TTAATACCAC AAGAAGATTC AGAAAGAGTC AGTACTTCAG AAAAGCAAAA AACGGGGCAA 
GTGCCTAAGA AAGACAATTC TCGAGGAGTT AAACGCAGTG CTAGTCCAGA TTACAGCAGG 
ACCAATTCTC CTAGCTCTGC TAAAAAACCT AAAGCACTTC AACACGCCGA AACTTCCTCG 
GAAAGTAACA AGCCACATAC TAAATCCAAG AGAAGACACT TAGACCAAGA ACAGCAGAAG 
TCTACACAAT TGCCATCAAC AAGCAAGGCT CACACCAGAA AGGGTGGAGC TGCTGGTAGC 
TCCCGAAGTC AGAAAAGGAA AAGGACAGAG AGTACGTCTT GTATAAAGAG TCGTTCAGCA 
GTTGAATCAA CTGGAGCTGA AGAGAAGTCA GCAAAATCCT CCAAGCTGGC TTCAAAATCG 
GTGACCTCAG CCAAAGCTGG GTGTAGCACC ATCACTGATT CTTCTTCTTC AGCTTCTACA 
TCCTCTTCCT CTTCTGCTGT TGCCTCTGCT TCTTCCACTG TTCCTCAGGG TGCCAGAGTG 
AAACAGGGTA AGGACCAGAA TAAGGCTAGA CGTTCACGTT CTGCGTCCAG CCCTAGTCCA 
AGAAGGAGCA GCAGGGACAA AGAACAGAGC AAAACAGGTG GCTCTTCAAA GTTTGACTGG 
GCTGCTCGAT TCAGTCCAAA AGTTAGTCTC CCTAAAACAA AACTGTCTCT ACCAGGCTCT 
TCTAAGTCAG AGACCTCAAA ACCTGGACCT TCAGGACTAC AGGCTAAGCT AGCAAGCCTA 
AGAAAATCCA CAAAGAAGCG CAGTGAATCA CCACCTGCTG AGCTCCCCAG TTTACGGCGG 
AGCACACGGC AAAAGACCAC GGGCTCCTGT GCGAGTACCA GTCGGCGGGG CTCTGGCCTG 
GGCAAAAGAG GAGCAGCTGA AGCTCGCCGA CAGGAGAAGA TGGCTGATCC TGACAACAAC 
CAGGATGGAG TTAACTCTTC AGCTGCACGT ACAGATGAGA CTCCCCAGGG AGCTGCAGCT 
TCTAGCTCTG TTGCTGGGGC TGTAGGTATG ACAACATCTG GAGAAAGTGA GTCAGATGAC 
TCTGAGATGG GAAGACTACA AGGTTCTAAA GCCCAGCAGC TTTTACAAGG TCTCCAAGCC 
ACTGATGAAA GTCAGCAACT ACAGGCAGTT ATTGAGATGT GCCAGCTGTT GGTCATGGGA 
AATGAAGAAA CACTGGGAGG ATTTCCAGTC AAGAGTGTTG TGCCAGCTTT GATAACATTG 
CTGCAGATGG AGCATAACTT TGACATCATG AACCATGCTT GTCGAGCCTT AACATACATG 
ATGGAAGCAC TTCCCAGATC ATCTGCTGTA GTGGTAGATG CAATTCCTGT CTTCTTGGAA 
AAGCTGCAAG TTATTCAGTG CATTGATGTG GCAGAGCAGG CACTGACAGC CCTAGAGATG 
CTATCACGCA GGCATAGTAA AGCCATTCTG CAGGCAGGTG GGTTGGCAGA CTGCTTGCTG 
TATCTGGAAT TCTTCAGTAT AAATGCGCAG AGGAATGCAC TAGCTATTGC TGCCAATTGC 
TGCCAGAGTA TAACACCTGA TGAGTTTCAC TTTGTGGCAG ACTCTTTGCC ACTGCTTACA 
CAAAGGTTAA CCCATCAGGA CAAGAAGTCT GTTGAAAGCA CTTGTCTCTG TTTTGCACGG 
CTGGTAGACA ATTTCCAGCA TGAGGAGAAC TTGCTGCAGC AGGTTGCTTC CAAGGACTTG 
TTAACAAACA TCCAGCAACT CTTGGTAGTG ACACCTCCTA TCCTGAGCTC AGGAATGTTC 
ATCATGGTGG TGCGCATGTT TTCCTTAATG TGTTCCAATT GTCCAACGCT TGCAGTCCAA 
CTTATGAAGC AAAACATTGC AGAAACACTT CACTTTCTCC TTTGTGGAGC CTCAAATGGG 
AGTTGTCAAG AACAAATTGA CCTTGTTCCA CGAAGTCCTC AAGAACTCTA TGAGCTTACT 
TCTCTTATCT GTGAACTGAT GCCTTGTCTG CCAAAAGAGG GGATATTTGC TGTTGATACT 
ATGCTAAAGA AAGGAAATGC ACAAAATACA GATGGTGCGA TATGGCAATG GCGAGATGAC 
AGGGGTCTCT GGCATCCCTA TAACAGGATT GATAGTCGAA TAATAGAGGC AGCTCATCAG 
GTTGGTGAGG ATGAGATAAG CCTGTCGACA CTTGGACGTG TCTATACTAT TGATTTTAAT 
TCTATGCAGC AAATAAATGA GGATACTGGA ACAGCACGTG CCATTCAGCG GAAACCAAAC 
CCTTTAGCCA ATACAAACAC TAGTGGACAT TCAGAATTGA AGAAGGATGA TGCTCGAGCA 
CAGCTAATGA AAGAAGACCC AGAATTGGCA AAATCCTTTA TCAAAACACT GTTTGGTGTT 
CTTTATGAAG TGTATAGTTC TTCAGCTGGA CCTGCTGTTA GACACAAGTG CCTTAGAGCA 
ATACTTAGAA TAATTTATTT TGCTGATGCT GAACTCCTGA AGGATGTGCT GAAAAACCAT 
GCTGTGTCAA GTCACATTGC CTCCATGCTG TCAAGTCAAG ACCTTAAGAT AGTAGTTGGA 
GCCCTGCAGA TGGCAGAAAT CTTAATGCAG AAGCTACCTG ACATTTTTAG TGTTTACTTC 
AGAAGAGAAG GAGTGATGCA TCAAGTGAAA AACTTAGCGG AGTCTGAGGC TTTGCTAACG 
AGCCCACCAA AAGTATGCAC TAATGGATCA GGAACACTGG GTACCACTAC AACTATAAGT 
ACTGGAACAG CCACTGCTGC CACTAATGCA GCTGCAGATT TGGGCTCTCC CAGCTTACAA 
CACAGCCGGG AGGATTCTTT AGATCTGAGC CCACAGGGAC GACTGAGTGA TGTTCTGAAG 
AGAAAAAGAC TGCCAAAACG AGGGCCAAGG AGACCAAAAT ATTCTCCCCC AAGAGATGAT 
GACAAAGTAG ACAATCAAGC AAAAAGCCCC ACGACTACTC AGTCTCCTAA ATCTTCTTTC 
TTAGCAAGTT TAAATCCCAA AACATGGGGA AGATTAAGTA CACAGTCTAA CAGTAATAAC 
ATTGAACCAG CAAGAACAGC AGGTGTAAGT GGTCTTGCAA GAGCTGCTTC TAAGGATACA 
ATTTCCAATA ACAGAGAAAA AATTAAGGGT TGGATTAAGG AGCAAGCCCA TAAATTTGTA 
GACCGTTATT TTAGTTCGGA AAACATGGAT GGAAGCAATC CTGCACTAAA TGTATTACAG 
AGACTTTGCA CTGCAACTGA ACAACTTAAC CTCCAGGTGG ATGGTGGAAC AGAGTGCCTT 
GTAGAAATCC GTAGCATTGT CTCGGAGTCT GACGTCTCCT CATTTGAAAT CCAGCATAGT 
GGATTTGTGA AACAACTGCT GCTTTATTTG ACATCTAAAA GTGAGAAAGA TGCTGTAAGC 
AGGGATATCA GATTGAAAAG ATTTCTTCAT GTATTTTTCT CCTCTCCACT TCCTGGAGAA 
GAACCCCTTG GAAGATTAGA GCCATTAGAA AATGCACCTT TGTTGGCATT AGTCCATAAA 
ATGAACAATT GCCTCAGTCA GATGGAGCAG TTTCCAGTGA AAGTGCATGA CTTCCCCAGT 
GGAAATGGAA CAGGGAGCAG TTTTTCTCTT AACAGAGGAT CCCAAGCTTT AAAATTCTTC 
AATACGCATC AGTTAAAATG CCAGCTGCAA AGACATCCAG ACTGTGCTAA TGTGAAACAG 
TGGAAGGGTG GACCTGTGAA GATTGATCCT CTGGCTTTGG TTCAAGCCAT TGAAAGATAT 
CTTGTTGTTA GAGGTTATGG AAGAGTTAGA GAAGATGATG AGGATAGTGA TGATGATGGG 
TCAGATGAAG AAATAGATGA ATCCTTGGCT GCTCAATTCT TAAATTCAGG GAATGTGAGA 
CATAGATTGC AGTTTTACAT TGGGGATCAC TTGCTACCAT ATAATATGAC TGTATATCAA 
GCAGTTAAGC TGTACAGTTT GCAAGCTGAG GAGGAGAGGG AATCGACTGA TGATGAAAGC 
AATCCATTAG GAAGAGCTGG GATTTGGACA AAAACACATA CCATTTGGTA CAAACCTGTA 
CGGGAGGATG AAGATGGCAA CAAAGACTGT GTTGGTGGTA AAAGAGGAAG AGCTCAAACT 
GCTCCCACAA AAACCTCACC TAGGAATTCT AAAAAGCATG ATGAGTTGTG GCACGATGGT 
GTATGCCCTT CAGTATTGAA TCCTTTAGAA GTTTACCTCA TATCTACTCC TCCTGAAAAC 
ATAACCTTTG AAGATCCTTC ATTAGATGTT ATTCTTCTTT TGAGAGTTTT ACATGCTATC 
AGTCGATACT GGTATTATTT GTATGATAAT GCAATATGCA AGGAGATTAT TCCAACTTCG 
GAATTTATCA ATAGTAAACT GACAGCAAAA GCAAACAGAC AGCTTCAAGA TCCACTGGTA 
ATTATGACAG GAAACATACC GACTTGGCTG ACAGAGCTTG GAAAAACATG CCCATTTTTC 
TTTCCATTTG ATACCCGGCA AATGCTGTTT TATGTAACTG CTTTTGATCG TGATCGAGCC 
ATGCAAAGAC TACTGGATAC TAATCCAGAA ATCAATCAAT CAGATTCTCA GGATAGCAGA 
GTGGCACCAC GACTGGACAG GAAAAAACGC ACTGTGAACA GAGATGAGCT GTTGAAACAG 
GCAGAATCTG TGATGCAGGA TCTAGGCAGT TCAAGAGCCA TGTTGGAAAT CCAGTATGAG 
AATGAAGTTG GCACAGGCCT GGGCCCTACA CTAGAGTTCT ATGCACTTGT ATCTCAGGAA 
CTACAGAGAG CAGACTTGGG CCTTTGGAGG GGAGAAGAAG TAACTTTAGC CAATCCAAAA 
GGAAGCCAAG AAGGTACCAA GTATATTCAT AATCTCCAAG GCCTTTTTGC ACTTCCTTTT 
GGTAGAACAG CCAAGCCTGC TCACATTGCA AAAGTTAAAA TGAAGTTCCG CTTTCTGGGA 
AAACTAATGG CTAAGGCAAT CATGGATTTT AGACTGGTGG ATCTTCCTCT TGGACTTCCT 
TTTTATAAAT GGATGTTACG CCAGGAAACT TCTTTGACGT CACATGACTT GTTCAGTATT 
GATCCAGTAG TAGCCAAATC AATATATCAC CTTGAAGATA TTGTAAGACA AAAGAAAAGA 
CTTGAGCAGG ACAAAACACA GACCAAAGAA AGTCTACAGT ATGCATTGGA AACCCTGACT 
ATGAATGGCT GCTCAGTGGA GGATCTAGGG CTGGATTTCA CACTTCCTGG ATTTCCTAAC 
ATCGAGCTGA AAAAAGGGGG AAAAGATACA CCAGTCACCA TCCACAACTT GGAGGAGTAC 
CTCAGATTGG TTATATTCTG GGCACTTAAC GAAGGTGTTG CCAGACAGTT TGATTCTTTC 
AGAGATGGAT TTGAATCAGT CTTCCCCCTC AGTCATCTTC AATACTTTTA TCCTGAGGAG 
TTGGAGCAAC TCTTATGTGG CAGCAAAACA GACACATGGG ATGCCAAGAC CTTAATGGAA 
TGTTGCAGGC CAGATCACGG TTATACACAT GACAGTCGAG CAGTGAAGTA TCTCTTTGAA 
ATTCTCAGTA GTTTTGATAG TGAGCAGCAG AGACTGTTTC TTCAGTTTGT GACCGGTAGT 
CCCAGGCTGC CAGTAGGAGG CTTTCGAAGT TTGAATCCTC CATTGACAAT TGTACGGAAG 
ACATTTGAGT CTACAGAGAA TCCAGATGAC TTCTTGCCCT CAGTAATGAC TTGTGTGAAC 
TATCTCAAAT TGCCGGACTA TTCAACTATT GAGATAATGC GTGAAAAACT CTTGATAGCT 
GCAAGAGAAG GGCAGCAGTC ATTCCATCTT TCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TRIP12 ( XM_015276972.1 ) cDNA ORF clone, Gallus gallus(chicken) -> XP_015132458.1 Gallus gallus thyroid hormone receptor interactor 12 (TRIP12), transcript variant X11, mRNA.

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CloneID	OGa39718
Clone ID Related Accession (Same CDS sequence)	XM_015276972.1 , XM_015276972.2
Accession Version	XM_015276972.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5970bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-03
Organism	Gallus gallus(chicken)
Product	E3 ubiquitin-protein ligase TRIP12 isoform X6
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_001471743.3) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Gallus gallus Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCCAACC GGCCTAATAA CAATCCAGGG GGGTCACTGC GACGTTCACA GAGGAACACT 
GCTGGGGCCC AGCCACAAGA CGACGCAGCA GGAGGAAGGT CACATTTAGG GCAGGCAAAA 
CATAAGGCAC ATAGCCCTCC TGAGAGTAGA AAATCTATTT CAAAGACACC CAAAGTGCAG 
TCTAATACTA CTTCTGAGCA GTCCAAGGGA CACTTTTCTA AAAGAGGCTG TAGTTCATCT 
GCTGTTTTAA TACCACAAGA AGATTCAGAA AGAGTCAGTA CTTCAGAAAA GCAAAAAACG 
GGGCAAGTGC CTAAGAAAGA CAATTCTCGA GGAGTTAAAC GCAGTGCTAG TCCAGATTAC 
AGCAGGACCA ATTCTCCTAG CTCTGCTAAA AAACCTAAAG CACTTCAACA CGCCGAAACT 
TCCTCGGAAA GTAACAAGCC ACATACTAAA TCCAAGAGAA GACACTTAGA CCAAGAACAG 
CAGAAGTCTA CACAATTGCC ATCAACAAGC AAGGCTCACA CCAGAAAGGG TGGAGCTGCT 
GGTAGCTCCC GAAGTCAGAA AAGGAAAAGG ACAGAGAGTA CGTCTTGTAT AAAGAGTCGT 
TCAGCAGTTG AATCAACTGG AGCTGAAGAG AAGTCAGCAA AATCCTCCAA GCTGGCTTCA 
AAATCGGTGA CCTCAGCCAA AGCTGGGTGT AGCACCATCA CTGATTCTTC TTCTTCAGCT 
TCTACATCCT CTTCCTCTTC TGCTGTTGCC TCTGCTTCTT CCACTGTTCC TCAGGGTGCC 
AGAGTGAAAC AGGGCCTAAG AAAATCCACA AAGAAGCGCA GTGAATCACC ACCTGCTGAG 
CTCCCCAGTT TACGGCGGAG CACACGGCAA AAGACCACGG GCTCCTGTGC GAGTACCAGT 
CGGCGGGGCT CTGGCCTGGG CAAAAGAGGA GCAGCTGAAG CTCGCCGACA GGAGAAGATG 
GCTGATCCTG ACAACAACCA GGATGGAGTT AACTCTTCAG CTGCACGTAC AGATGAGACT 
CCCCAGGGAG CTGCAGCTTC TAGCTCTGTT GCTGGGGCTG TAGGTATGAC AACATCTGGA 
GAAAGTGAGT CAGATGACTC TGAGATGGGA AGACTACAAG CTCTGTTAGA GGCTAGGGGT 
CTTCCTCCTC ACCTGTTTGG CCCTCTTGGT CCTCGGATGT CGCAGCTCTT CCACAGGACA 
ATTGGAAGTG GAGCTAGTTC TAAAGCCCAG CAGCTTTTAC AAGGTCTCCA AGCCACTGAT 
GAAAGTCAGC AACTACAGGC AGTTATTGAG ATGTGCCAGC TGTTGGTCAT GGGAAATGAA 
GAAACACTGG GAGGATTTCC AGTCAAGAGT GTTGTGCCAG CTTTGATAAC ATTGCTGCAG 
ATGGAGCATA ACTTTGACAT CATGAACCAT GCTTGTCGAG CCTTAACATA CATGATGGAA 
GCACTTCCCA GATCATCTGC TGTAGTGGTA GATGCAATTC CTGTCTTCTT GGAAAAGCTG 
CAAGTTATTC AGTGCATTGA TGTGGCAGAG CAGGCACTGA CAGCCCTAGA GATGCTATCA 
CGCAGGCATA GTAAAGCCAT TCTGCAGGCA GGTGGGTTGG CAGACTGCTT GCTGTATCTG 
GAATTCTTCA GTATAAATGC GCAGAGGAAT GCACTAGCTA TTGCTGCCAA TTGCTGCCAG 
AGTATAACAC CTGATGAGTT TCACTTTGTG GCAGACTCTT TGCCACTGCT TACACAAAGG 
TTAACCCATC AGGACAAGAA GTCTGTTGAA AGCACTTGTC TCTGTTTTGC ACGGCTGGTA 
GACAATTTCC AGCATGAGGA GAACTTGCTG CAGCAGGTTG CTTCCAAGGA CTTGTTAACA 
AACATCCAGC AACTCTTGGT AGTGACACCT CCTATCCTGA GCTCAGGAAT GTTCATCATG 
GTGGTGCGCA TGTTTTCCTT AATGTGTTCC AATTGTCCAA CGCTTGCAGT CCAACTTATG 
AAGCAAAACA TTGCAGAAAC ACTTCACTTT CTCCTTTGTG GAGCCTCAAA TGGGAGTTGT 
CAAGAACAAA TTGACCTTGT TCCACGAAGT CCTCAAGAAC TCTATGAGCT TACTTCTCTT 
ATCTGTGAAC TGATGCCTTG TCTGCCAAAA GAGGGGATAT TTGCTGTTGA TACTATGCTA 
AAGAAAGGAA ATGCACAAAA TACAGATGGT GCGATATGGC AATGGCGAGA TGACAGGGGT 
CTCTGGCATC CCTATAACAG GATTGATAGT CGAATAATAG AGGCAGCTCA TCAGGTTGGT 
GAGGATGAGA TAAGCCTGTC GACACTTGGA CGTGTCTATA CTATTGATTT TAATTCTATG 
CAGCAAATAA ATGAGGATAC TGGAACAGCA CGTGCCATTC AGCGGAAACC AAACCCTTTA 
GCCAATACAA ACACTAGTGG ACATTCAGAA TTGAAGAAGG ATGATGCTCG AGCACAGCTA 
ATGAAAGAAG ACCCAGAATT GGCAAAATCC TTTATCAAAA CACTGTTTGG TGTTCTTTAT 
GAAGTGTATA GTTCTTCAGC TGGACCTGCT GTTAGACACA AGTGCCTTAG AGCAATACTT 
AGAATAATTT ATTTTGCTGA TGCTGAACTC CTGAAGGATG TGCTGAAAAA CCATGCTGTG 
TCAAGTCACA TTGCCTCCAT GCTGTCAAGT CAAGACCTTA AGATAGTAGT TGGAGCCCTG 
CAGATGGCAG AAATCTTAAT GCAGAAGCTA CCTGACATTT TTAGTGTTTA CTTCAGAAGA 
GAAGGAGTGA TGCATCAAGT GAAAAACTTA GCGGAGTCTG AGGCTTTGCT AACGAGCCCA 
CCAAAAGTAT GCACTAATGG ATCAGGAACA CTGGGTACCA CTACAACTAT AAGTACTGGA 
ACAGCCACTG CTGCCACTAA TGCAGCTGCA GATTTGGGCT CTCCCAGCTT ACAACACAGC 
CGGGAGGATT CTTTAGATCT GAGCCCACAG GGACGACTGA GTGATGTTCT GAAGAGAAAA 
AGACTGCCAA AACGAGGGCC AAGGAGACCA AAATATTCTC CCCCAAGAGA TGATGACAAA 
GTAGACAATC AAGCAAAAAG CCCCACGACT ACTCAGTCTC CTAAATCTTC TTTCTTAGCA 
AGTTTAAATC CCAAAACATG GGGAAGATTA AGTACACAGT CTAACAGTAA TAACATTGAA 
CCAGCAAGAA CAGCAGGTGT AAGTGGTCTT GCAAGAGCTG CTTCTAAGGA TACAATTTCC 
AATAACAGAG AAAAAATTAA GGGTTGGATT AAGGAGCAAG CCCATAAATT TGTAGACCGT 
TATTTTAGTT CGGAAAACAT GGATGGAAGC AATCCTGCAC TAAATGTATT ACAGAGACTT 
TGCACTGCAA CTGAACAACT TAACCTCCAG GTGGATGGTG GAACAGAGTG CCTTGTAGAA 
ATCCGTAGCA TTGTCTCGGA GTCTGACGTC TCCTCATTTG AAATCCAGCA TAGTGGATTT 
GTGAAACAAC TGCTGCTTTA TTTGACATCT AAAAGTGAGA AAGATGCTGT AAGCAGGGAT 
ATCAGATTGA AAAGATTTCT TCATGTATTT TTCTCCTCTC CACTTCCTGG AGAAGAACCC 
CTTGGAAGAT TAGAGCCATT AGAAAATGCA CCTTTGTTGG CATTAGTCCA TAAAATGAAC 
AATTGCCTCA GTCAGATGGA GCAGTTTCCA GTGAAAGTGC ATGACTTCCC CAGTGGAAAT 
GGAACAGGGA GCAGTTTTTC TCTTAACAGA GGATCCCAAG CTTTAAAATT CTTCAATACG 
CATCAGTTAA AATGCCAGCT GCAAAGACAT CCAGACTGTG CTAATGTGAA ACAGTGGAAG 
GGTGGACCTG TGAAGATTGA TCCTCTGGCT TTGGTTCAAG CCATTGAAAG ATATCTTGTT 
GTTAGAGGTT ATGGAAGAGT TAGAGAAGAT GATGAGGATA GTGATGATGA TGGGTCAGAT 
GAAGAAATAG ATGAATCCTT GGCTGCTCAA TTCTTAAATT CAGGGAATGT GAGACATAGA 
TTGCAGTTTT ACATTGGGGA TCACTTGCTA CCATATAATA TGACTGTATA TCAAGCAGTT 
AAGCTGTACA GTTTGCAAGC TGAGGAGGAG AGGGAATCGA CTGATGATGA AAGCAATCCA 
TTAGGAAGAG CTGGGATTTG GACAAAAACA CATACCATTT GGTACAAACC TGTACGGGAG 
GATGAAGATG GCAACAAAGA CTGTGTTGGT GGTAAAAGAG GAAGAGCTCA AACTGCTCCC 
ACAAAAACCT CACCTAGGAA TTCTAAAAAG CATGATGAGT TGTGGCACGA TGGTGTATGC 
CCTTCAGTAT TGAATCCTTT AGAAGTTTAC CTCATATCTA CTCCTCCTGA AAACATAACC 
TTTGAAGATC CTTCATTAGA TGTTATTCTT CTTTTGAGAG TTTTACATGC TATCAGTCGA 
TACTGGTATT ATTTGTATGA TAATGCAATA TGCAAGGAGA TTATTCCAAC TTCGGAATTT 
ATCAATAGTA AACTGACAGC AAAAGCAAAC AGACAGCTTC AAGATCCACT GGTAATTATG 
ACAGGAAACA TACCGACTTG GCTGACAGAG CTTGGAAAAA CATGCCCATT TTTCTTTCCA 
TTTGATACCC GGCAAATGCT GTTTTATGTA ACTGCTTTTG ATCGTGATCG AGCCATGCAA 
AGACTACTGG ATACTAATCC AGAAATCAAT CAATCAGATT CTCAGGATAG CAGAGTGGCA 
CCACGACTGG ACAGGAAAAA ACGCACTGTG AACAGAGATG AGCTGTTGAA ACAGGCAGAA 
TCTGTGATGC AGGATCTAGG CAGTTCAAGA GCCATGTTGG AAATCCAGTA TGAGAATGAA 
GTTGGCACAG GCCTGGGCCC TACACTAGAG TTCTATGCAC TTGTATCTCA GGAACTACAG 
AGAGCAGACT TGGGCCTTTG GAGGGGAGAA GAAGTAACTT TAGCCAATCC AAAAGGAAGC 
CAAGAAGGTA CCAAGTATAT TCATAATCTC CAAGGCCTTT TTGCACTTCC TTTTGGTAGA 
ACAGCCAAGC CTGCTCACAT TGCAAAAGTT AAAATGAAGT TCCGCTTTCT GGGAAAACTA 
ATGGCTAAGG CAATCATGGA TTTTAGACTG GTGGATCTTC CTCTTGGACT TCCTTTTTAT 
AAATGGATGT TACGCCAGGA AACTTCTTTG ACGTCACATG ACTTGTTCAG TATTGATCCA 
GTAGTAGCCA AATCAATATA TCACCTTGAA GATATTGTAA GACAAAAGAA AAGACTTGAG 
CAGGACAAAA CACAGACCAA AGAAAGTCTA CAGTATGCAT TGGAAACCCT GACTATGAAT 
GGCTGCTCAG TGGAGGATCT AGGGCTGGAT TTCACACTTC CTGGATTTCC TAACATCGAG 
CTGAAAAAAG GGGGAAAAGA TACACCAGTC ACCATCCACA ACTTGGAGGA GTACCTCAGA 
TTGGTTATAT TCTGGGCACT TAACGAAGGT GTTGCCAGAC AGTTTGATTC TTTCAGAGAT 
GGATTTGAAT CAGTCTTCCC CCTCAGTCAT CTTCAATACT TTTATCCTGA GGAGTTGGAG 
CAACTCTTAT GTGGCAGCAA AACAGACACA TGGGATGCCA AGACCTTAAT GGAATGTTGC 
AGGCCAGATC ACGGTTATAC ACATGACAGT CGAGCAGTGA AGTATCTCTT TGAAATTCTC 
AGTAGTTTTG ATAGTGAGCA GCAGAGACTG TTTCTTCAGT TTGTGACCGG TAGTCCCAGG 
CTGCCAGTAG GAGGCTTTCG AAGTTTGAAT CCTCCATTGA CAATTGTACG GAAGACATTT 
GAGTCTACAG AGAATCCAGA TGACTTCTTG CCCTCAGTAA TGACTTGTGT GAACTATCTC 
AAATTGCCGG ACTATTCAAC TATTGAGATA ATGCGTGAAA AACTCTTGAT AGCTGCAAGA 
GAAGGGCAGC AGTCATTCCA TCTTTCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015132458.1
CDS	150..6119
Translation	MSNRPNNNPGGSLRRSQRNTAGAQPQDDAAGGRSHLGQAKHKAHSPPESRKSISKTPKVQSNTTSEQSKGHFSKRGCSSSAVLIPQEDSERVSTSEKQKTGQVPKKDNSRGVKRSASPDYSRTNSPSSAKKPKALQHAETSSESNKPHTKSKRRHLDQEQQKSTQLPSTSKAHTRKGGAAGSSRSQKRKRTESTSCIKSRSAVESTGAEEKSAKSSKLASKSVTSAKAGCSTITDSSSSASTSSSSSAVASASSTVPQGARVKQGLRKSTKKRSESPPAELPSLRRSTRQKTTGSCASTSRRGSGLGKRGAAEARRQEKMADPDNNQDGVNSSAARTDETPQGAAASSSVAGAVGMTTSGESESDDSEMGRLQALLEARGLPPHLFGPLGPRMSQLFHRTIGSGASSKAQQLLQGLQATDESQQLQAVIEMCQLLVMGNEETLGGFPVKSVVPALITLLQMEHNFDIMNHACRALTYMMEALPRSSAVVVDAIPVFLEKLQVIQCIDVAEQALTALEMLSRRHSKAILQAGGLADCLLYLEFFSINAQRNALAIAANCCQSITPDEFHFVADSLPLLTQRLTHQDKKSVESTCLCFARLVDNFQHEENLLQQVASKDLLTNIQQLLVVTPPILSSGMFIMVVRMFSLMCSNCPTLAVQLMKQNIAETLHFLLCGASNGSCQEQIDLVPRSPQELYELTSLICELMPCLPKEGIFAVDTMLKKGNAQNTDGAIWQWRDDRGLWHPYNRIDSRIIEAAHQVGEDEISLSTLGRVYTIDFNSMQQINEDTGTARAIQRKPNPLANTNTSGHSELKKDDARAQLMKEDPELAKSFIKTLFGVLYEVYSSSAGPAVRHKCLRAILRIIYFADAELLKDVLKNHAVSSHIASMLSSQDLKIVVGALQMAEILMQKLPDIFSVYFRREGVMHQVKNLAESEALLTSPPKVCTNGSGTLGTTTTISTGTATAATNAAADLGSPSLQHSREDSLDLSPQGRLSDVLKRKRLPKRGPRRPKYSPPRDDDKVDNQAKSPTTTQSPKSSFLASLNPKTWGRLSTQSNSNNIEPARTAGVSGLARAASKDTISNNREKIKGWIKEQAHKFVDRYFSSENMDGSNPALNVLQRLCTATEQLNLQVDGGTECLVEIRSIVSESDVSSFEIQHSGFVKQLLLYLTSKSEKDAVSRDIRLKRFLHVFFSSPLPGEEPLGRLEPLENAPLLALVHKMNNCLSQMEQFPVKVHDFPSGNGTGSSFSLNRGSQALKFFNTHQLKCQLQRHPDCANVKQWKGGPVKIDPLALVQAIERYLVVRGYGRVREDDEDSDDDGSDEEIDESLAAQFLNSGNVRHRLQFYIGDHLLPYNMTVYQAVKLYSLQAEEERESTDDESNPLGRAGIWTKTHTIWYKPVREDEDGNKDCVGGKRGRAQTAPTKTSPRNSKKHDELWHDGVCPSVLNPLEVYLISTPPENITFEDPSLDVILLLRVLHAISRYWYYLYDNAICKEIIPTSEFINSKLTAKANRQLQDPLVIMTGNIPTWLTELGKTCPFFFPFDTRQMLFYVTAFDRDRAMQRLLDTNPEINQSDSQDSRVAPRLDRKKRTVNRDELLKQAESVMQDLGSSRAMLEIQYENEVGTGLGPTLEFYALVSQELQRADLGLWRGEEVTLANPKGSQEGTKYIHNLQGLFALPFGRTAKPAHIAKVKMKFRFLGKLMAKAIMDFRLVDLPLGLPFYKWMLRQETSLTSHDLFSIDPVVAKSIYHLEDIVRQKKRLEQDKTQTKESLQYALETLTMNGCSVEDLGLDFTLPGFPNIELKKGGKDTPVTIHNLEEYLRLVIFWALNEGVARQFDSFRDGFESVFPLSHLQYFYPEELEQLLCGSKTDTWDAKTLMECCRPDHGYTHDSRAVKYLFEILSSFDSEQQRLFLQFVTGSPRLPVGGFRSLNPPLTIVRKTFESTENPDDFLPSVMTCVNYLKLPDYSTIEIMREKLLIAAREGQQSFHLS

Target ORF information:

RefSeq Version	XM_015276972.1
Organism	Gallus gallus(chicken)
Definition	Gallus gallus thyroid hormone receptor interactor 12 (TRIP12), transcript variant X11, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015276972.1

ORF Insert Sequence:

ATGTCCAACC GGCCTAATAA CAATCCAGGG GGGTCACTGC GACGTTCACA GAGGAACACT 
GCTGGGGCCC AGCCACAAGA CGACGCAGCA GGAGGAAGGT CACATTTAGG GCAGGCAAAA 
CATAAGGCAC ATAGCCCTCC TGAGAGTAGA AAATCTATTT CAAAGACACC CAAAGTGCAG 
TCTAATACTA CTTCTGAGCA GTCCAAGGGA CACTTTTCTA AAAGAGGCTG TAGTTCATCT 
GCTGTTTTAA TACCACAAGA AGATTCAGAA AGAGTCAGTA CTTCAGAAAA GCAAAAAACG 
GGGCAAGTGC CTAAGAAAGA CAATTCTCGA GGAGTTAAAC GCAGTGCTAG TCCAGATTAC 
AGCAGGACCA ATTCTCCTAG CTCTGCTAAA AAACCTAAAG CACTTCAACA CGCCGAAACT 
TCCTCGGAAA GTAACAAGCC ACATACTAAA TCCAAGAGAA GACACTTAGA CCAAGAACAG 
CAGAAGTCTA CACAATTGCC ATCAACAAGC AAGGCTCACA CCAGAAAGGG TGGAGCTGCT 
GGTAGCTCCC GAAGTCAGAA AAGGAAAAGG ACAGAGAGTA CGTCTTGTAT AAAGAGTCGT 
TCAGCAGTTG AATCAACTGG AGCTGAAGAG AAGTCAGCAA AATCCTCCAA GCTGGCTTCA 
AAATCGGTGA CCTCAGCCAA AGCTGGGTGT AGCACCATCA CTGATTCTTC TTCTTCAGCT 
TCTACATCCT CTTCCTCTTC TGCTGTTGCC TCTGCTTCTT CCACTGTTCC TCAGGGTGCC 
AGAGTGAAAC AGGGCCTAAG AAAATCCACA AAGAAGCGCA GTGAATCACC ACCTGCTGAG 
CTCCCCAGTT TACGGCGGAG CACACGGCAA AAGACCACGG GCTCCTGTGC GAGTACCAGT 
CGGCGGGGCT CTGGCCTGGG CAAAAGAGGA GCAGCTGAAG CTCGCCGACA GGAGAAGATG 
GCTGATCCTG ACAACAACCA GGATGGAGTT AACTCTTCAG CTGCACGTAC AGATGAGACT 
CCCCAGGGAG CTGCAGCTTC TAGCTCTGTT GCTGGGGCTG TAGGTATGAC AACATCTGGA 
GAAAGTGAGT CAGATGACTC TGAGATGGGA AGACTACAAG CTCTGTTAGA GGCTAGGGGT 
CTTCCTCCTC ACCTGTTTGG CCCTCTTGGT CCTCGGATGT CGCAGCTCTT CCACAGGACA 
ATTGGAAGTG GAGCTAGTTC TAAAGCCCAG CAGCTTTTAC AAGGTCTCCA AGCCACTGAT 
GAAAGTCAGC AACTACAGGC AGTTATTGAG ATGTGCCAGC TGTTGGTCAT GGGAAATGAA 
GAAACACTGG GAGGATTTCC AGTCAAGAGT GTTGTGCCAG CTTTGATAAC ATTGCTGCAG 
ATGGAGCATA ACTTTGACAT CATGAACCAT GCTTGTCGAG CCTTAACATA CATGATGGAA 
GCACTTCCCA GATCATCTGC TGTAGTGGTA GATGCAATTC CTGTCTTCTT GGAAAAGCTG 
CAAGTTATTC AGTGCATTGA TGTGGCAGAG CAGGCACTGA CAGCCCTAGA GATGCTATCA 
CGCAGGCATA GTAAAGCCAT TCTGCAGGCA GGTGGGTTGG CAGACTGCTT GCTGTATCTG 
GAATTCTTCA GTATAAATGC GCAGAGGAAT GCACTAGCTA TTGCTGCCAA TTGCTGCCAG 
AGTATAACAC CTGATGAGTT TCACTTTGTG GCAGACTCTT TGCCACTGCT TACACAAAGG 
TTAACCCATC AGGACAAGAA GTCTGTTGAA AGCACTTGTC TCTGTTTTGC ACGGCTGGTA 
GACAATTTCC AGCATGAGGA GAACTTGCTG CAGCAGGTTG CTTCCAAGGA CTTGTTAACA 
AACATCCAGC AACTCTTGGT AGTGACACCT CCTATCCTGA GCTCAGGAAT GTTCATCATG 
GTGGTGCGCA TGTTTTCCTT AATGTGTTCC AATTGTCCAA CGCTTGCAGT CCAACTTATG 
AAGCAAAACA TTGCAGAAAC ACTTCACTTT CTCCTTTGTG GAGCCTCAAA TGGGAGTTGT 
CAAGAACAAA TTGACCTTGT TCCACGAAGT CCTCAAGAAC TCTATGAGCT TACTTCTCTT 
ATCTGTGAAC TGATGCCTTG TCTGCCAAAA GAGGGGATAT TTGCTGTTGA TACTATGCTA 
AAGAAAGGAA ATGCACAAAA TACAGATGGT GCGATATGGC AATGGCGAGA TGACAGGGGT 
CTCTGGCATC CCTATAACAG GATTGATAGT CGAATAATAG AGGCAGCTCA TCAGGTTGGT 
GAGGATGAGA TAAGCCTGTC GACACTTGGA CGTGTCTATA CTATTGATTT TAATTCTATG 
CAGCAAATAA ATGAGGATAC TGGAACAGCA CGTGCCATTC AGCGGAAACC AAACCCTTTA 
GCCAATACAA ACACTAGTGG ACATTCAGAA TTGAAGAAGG ATGATGCTCG AGCACAGCTA 
ATGAAAGAAG ACCCAGAATT GGCAAAATCC TTTATCAAAA CACTGTTTGG TGTTCTTTAT 
GAAGTGTATA GTTCTTCAGC TGGACCTGCT GTTAGACACA AGTGCCTTAG AGCAATACTT 
AGAATAATTT ATTTTGCTGA TGCTGAACTC CTGAAGGATG TGCTGAAAAA CCATGCTGTG 
TCAAGTCACA TTGCCTCCAT GCTGTCAAGT CAAGACCTTA AGATAGTAGT TGGAGCCCTG 
CAGATGGCAG AAATCTTAAT GCAGAAGCTA CCTGACATTT TTAGTGTTTA CTTCAGAAGA 
GAAGGAGTGA TGCATCAAGT GAAAAACTTA GCGGAGTCTG AGGCTTTGCT AACGAGCCCA 
CCAAAAGTAT GCACTAATGG ATCAGGAACA CTGGGTACCA CTACAACTAT AAGTACTGGA 
ACAGCCACTG CTGCCACTAA TGCAGCTGCA GATTTGGGCT CTCCCAGCTT ACAACACAGC 
CGGGAGGATT CTTTAGATCT GAGCCCACAG GGACGACTGA GTGATGTTCT GAAGAGAAAA 
AGACTGCCAA AACGAGGGCC AAGGAGACCA AAATATTCTC CCCCAAGAGA TGATGACAAA 
GTAGACAATC AAGCAAAAAG CCCCACGACT ACTCAGTCTC CTAAATCTTC TTTCTTAGCA 
AGTTTAAATC CCAAAACATG GGGAAGATTA AGTACACAGT CTAACAGTAA TAACATTGAA 
CCAGCAAGAA CAGCAGGTGT AAGTGGTCTT GCAAGAGCTG CTTCTAAGGA TACAATTTCC 
AATAACAGAG AAAAAATTAA GGGTTGGATT AAGGAGCAAG CCCATAAATT TGTAGACCGT 
TATTTTAGTT CGGAAAACAT GGATGGAAGC AATCCTGCAC TAAATGTATT ACAGAGACTT 
TGCACTGCAA CTGAACAACT TAACCTCCAG GTGGATGGTG GAACAGAGTG CCTTGTAGAA 
ATCCGTAGCA TTGTCTCGGA GTCTGACGTC TCCTCATTTG AAATCCAGCA TAGTGGATTT 
GTGAAACAAC TGCTGCTTTA TTTGACATCT AAAAGTGAGA AAGATGCTGT AAGCAGGGAT 
ATCAGATTGA AAAGATTTCT TCATGTATTT TTCTCCTCTC CACTTCCTGG AGAAGAACCC 
CTTGGAAGAT TAGAGCCATT AGAAAATGCA CCTTTGTTGG CATTAGTCCA TAAAATGAAC 
AATTGCCTCA GTCAGATGGA GCAGTTTCCA GTGAAAGTGC ATGACTTCCC CAGTGGAAAT 
GGAACAGGGA GCAGTTTTTC TCTTAACAGA GGATCCCAAG CTTTAAAATT CTTCAATACG 
CATCAGTTAA AATGCCAGCT GCAAAGACAT CCAGACTGTG CTAATGTGAA ACAGTGGAAG 
GGTGGACCTG TGAAGATTGA TCCTCTGGCT TTGGTTCAAG CCATTGAAAG ATATCTTGTT 
GTTAGAGGTT ATGGAAGAGT TAGAGAAGAT GATGAGGATA GTGATGATGA TGGGTCAGAT 
GAAGAAATAG ATGAATCCTT GGCTGCTCAA TTCTTAAATT CAGGGAATGT GAGACATAGA 
TTGCAGTTTT ACATTGGGGA TCACTTGCTA CCATATAATA TGACTGTATA TCAAGCAGTT 
AAGCTGTACA GTTTGCAAGC TGAGGAGGAG AGGGAATCGA CTGATGATGA AAGCAATCCA 
TTAGGAAGAG CTGGGATTTG GACAAAAACA CATACCATTT GGTACAAACC TGTACGGGAG 
GATGAAGATG GCAACAAAGA CTGTGTTGGT GGTAAAAGAG GAAGAGCTCA AACTGCTCCC 
ACAAAAACCT CACCTAGGAA TTCTAAAAAG CATGATGAGT TGTGGCACGA TGGTGTATGC 
CCTTCAGTAT TGAATCCTTT AGAAGTTTAC CTCATATCTA CTCCTCCTGA AAACATAACC 
TTTGAAGATC CTTCATTAGA TGTTATTCTT CTTTTGAGAG TTTTACATGC TATCAGTCGA 
TACTGGTATT ATTTGTATGA TAATGCAATA TGCAAGGAGA TTATTCCAAC TTCGGAATTT 
ATCAATAGTA AACTGACAGC AAAAGCAAAC AGACAGCTTC AAGATCCACT GGTAATTATG 
ACAGGAAACA TACCGACTTG GCTGACAGAG CTTGGAAAAA CATGCCCATT TTTCTTTCCA 
TTTGATACCC GGCAAATGCT GTTTTATGTA ACTGCTTTTG ATCGTGATCG AGCCATGCAA 
AGACTACTGG ATACTAATCC AGAAATCAAT CAATCAGATT CTCAGGATAG CAGAGTGGCA 
CCACGACTGG ACAGGAAAAA ACGCACTGTG AACAGAGATG AGCTGTTGAA ACAGGCAGAA 
TCTGTGATGC AGGATCTAGG CAGTTCAAGA GCCATGTTGG AAATCCAGTA TGAGAATGAA 
GTTGGCACAG GCCTGGGCCC TACACTAGAG TTCTATGCAC TTGTATCTCA GGAACTACAG 
AGAGCAGACT TGGGCCTTTG GAGGGGAGAA GAAGTAACTT TAGCCAATCC AAAAGGAAGC 
CAAGAAGGTA CCAAGTATAT TCATAATCTC CAAGGCCTTT TTGCACTTCC TTTTGGTAGA 
ACAGCCAAGC CTGCTCACAT TGCAAAAGTT AAAATGAAGT TCCGCTTTCT GGGAAAACTA 
ATGGCTAAGG CAATCATGGA TTTTAGACTG GTGGATCTTC CTCTTGGACT TCCTTTTTAT 
AAATGGATGT TACGCCAGGA AACTTCTTTG ACGTCACATG ACTTGTTCAG TATTGATCCA 
GTAGTAGCCA AATCAATATA TCACCTTGAA GATATTGTAA GACAAAAGAA AAGACTTGAG 
CAGGACAAAA CACAGACCAA AGAAAGTCTA CAGTATGCAT TGGAAACCCT GACTATGAAT 
GGCTGCTCAG TGGAGGATCT AGGGCTGGAT TTCACACTTC CTGGATTTCC TAACATCGAG 
CTGAAAAAAG GGGGAAAAGA TACACCAGTC ACCATCCACA ACTTGGAGGA GTACCTCAGA 
TTGGTTATAT TCTGGGCACT TAACGAAGGT GTTGCCAGAC AGTTTGATTC TTTCAGAGAT 
GGATTTGAAT CAGTCTTCCC CCTCAGTCAT CTTCAATACT TTTATCCTGA GGAGTTGGAG 
CAACTCTTAT GTGGCAGCAA AACAGACACA TGGGATGCCA AGACCTTAAT GGAATGTTGC 
AGGCCAGATC ACGGTTATAC ACATGACAGT CGAGCAGTGA AGTATCTCTT TGAAATTCTC 
AGTAGTTTTG ATAGTGAGCA GCAGAGACTG TTTCTTCAGT TTGTGACCGG TAGTCCCAGG 
CTGCCAGTAG GAGGCTTTCG AAGTTTGAAT CCTCCATTGA CAATTGTACG GAAGACATTT 
GAGTCTACAG AGAATCCAGA TGACTTCTTG CCCTCAGTAA TGACTTGTGT GAACTATCTC 
AAATTGCCGG ACTATTCAAC TATTGAGATA ATGCGTGAAA AACTCTTGAT AGCTGCAAGA 
GAAGGGCAGC AGTCATTCCA TCTTTCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TRIP12 ( XM_015276972.2 ) cDNA ORF clone, Gallus gallus(chicken) -> XP_015132458.1 Gallus gallus thyroid hormone receptor interactor 12 (TRIP12), transcript variant X12, mRNA.

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CloneID	OGa39718
Clone ID Related Accession (Same CDS sequence)	XM_015276972.1 , XM_015276972.2
Accession Version	XM_015276972.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5970bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-16
Organism	Gallus gallus(chicken)
Product	E3 ubiquitin-protein ligase TRIP12 isoform X8
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006096.5) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On May 17, 2018 this sequence version replaced XM_015276972.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Gallus gallus Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCCAACC GGCCTAATAA CAATCCAGGG GGGTCACTGC GACGTTCACA GAGGAACACT 
GCTGGGGCCC AGCCACAAGA CGACGCAGCA GGAGGAAGGT CACATTTAGG GCAGGCAAAA 
CATAAGGCAC ATAGCCCTCC TGAGAGTAGA AAATCTATTT CAAAGACACC CAAAGTGCAG 
TCTAATACTA CTTCTGAGCA GTCCAAGGGA CACTTTTCTA AAAGAGGCTG TAGTTCATCT 
GCTGTTTTAA TACCACAAGA AGATTCAGAA AGAGTCAGTA CTTCAGAAAA GCAAAAAACG 
GGGCAAGTGC CTAAGAAAGA CAATTCTCGA GGAGTTAAAC GCAGTGCTAG TCCAGATTAC 
AGCAGGACCA ATTCTCCTAG CTCTGCTAAA AAACCTAAAG CACTTCAACA CGCCGAAACT 
TCCTCGGAAA GTAACAAGCC ACATACTAAA TCCAAGAGAA GACACTTAGA CCAAGAACAG 
CAGAAGTCTA CACAATTGCC ATCAACAAGC AAGGCTCACA CCAGAAAGGG TGGAGCTGCT 
GGTAGCTCCC GAAGTCAGAA AAGGAAAAGG ACAGAGAGTA CGTCTTGTAT AAAGAGTCGT 
TCAGCAGTTG AATCAACTGG AGCTGAAGAG AAGTCAGCAA AATCCTCCAA GCTGGCTTCA 
AAATCGGTGA CCTCAGCCAA AGCTGGGTGT AGCACCATCA CTGATTCTTC TTCTTCAGCT 
TCTACATCCT CTTCCTCTTC TGCTGTTGCC TCTGCTTCTT CCACTGTTCC TCAGGGTGCC 
AGAGTGAAAC AGGGCCTAAG AAAATCCACA AAGAAGCGCA GTGAATCACC ACCTGCTGAG 
CTCCCCAGTT TACGGCGGAG CACACGGCAA AAGACCACGG GCTCCTGTGC GAGTACCAGT 
CGGCGGGGCT CTGGCCTGGG CAAAAGAGGA GCAGCTGAAG CTCGCCGACA GGAGAAGATG 
GCTGATCCTG ACAACAACCA GGATGGAGTT AACTCTTCAG CTGCACGTAC AGATGAGACT 
CCCCAGGGAG CTGCAGCTTC TAGCTCTGTT GCTGGGGCTG TAGGTATGAC AACATCTGGA 
GAAAGTGAGT CAGATGACTC TGAGATGGGA AGACTACAAG CTCTGTTAGA GGCTAGGGGT 
CTTCCTCCTC ACCTGTTTGG CCCTCTTGGT CCTCGGATGT CGCAGCTCTT CCACAGGACA 
ATTGGAAGTG GAGCTAGTTC TAAAGCCCAG CAGCTTTTAC AAGGTCTCCA AGCCACTGAT 
GAAAGTCAGC AACTACAGGC AGTTATTGAG ATGTGCCAGC TGTTGGTCAT GGGAAATGAA 
GAAACACTGG GAGGATTTCC AGTCAAGAGT GTTGTGCCAG CTTTGATAAC ATTGCTGCAG 
ATGGAGCATA ACTTTGACAT CATGAACCAT GCTTGTCGAG CCTTAACATA CATGATGGAA 
GCACTTCCCA GATCATCTGC TGTAGTGGTA GATGCAATTC CTGTCTTCTT GGAAAAGCTG 
CAAGTTATTC AGTGCATTGA TGTGGCAGAG CAGGCACTGA CAGCCCTAGA GATGCTATCA 
CGCAGGCATA GTAAAGCCAT TCTGCAGGCA GGTGGGTTGG CAGACTGCTT GCTGTATCTG 
GAATTCTTCA GTATAAATGC GCAGAGGAAT GCACTAGCTA TTGCTGCCAA TTGCTGCCAG 
AGTATAACAC CTGATGAGTT TCACTTTGTG GCAGACTCTT TGCCACTGCT TACACAAAGG 
TTAACCCATC AGGACAAGAA GTCTGTTGAA AGCACTTGTC TCTGTTTTGC ACGGCTGGTA 
GACAATTTCC AGCATGAGGA GAACTTGCTG CAGCAGGTTG CTTCCAAGGA CTTGTTAACA 
AACATCCAGC AACTCTTGGT AGTGACACCT CCTATCCTGA GCTCAGGAAT GTTCATCATG 
GTGGTGCGCA TGTTTTCCTT AATGTGTTCC AATTGTCCAA CGCTTGCAGT CCAACTTATG 
AAGCAAAACA TTGCAGAAAC ACTTCACTTT CTCCTTTGTG GAGCCTCAAA TGGGAGTTGT 
CAAGAACAAA TTGACCTTGT TCCACGAAGT CCTCAAGAAC TCTATGAGCT TACTTCTCTT 
ATCTGTGAAC TGATGCCTTG TCTGCCAAAA GAGGGGATAT TTGCTGTTGA TACTATGCTA 
AAGAAAGGAA ATGCACAAAA TACAGATGGT GCGATATGGC AATGGCGAGA TGACAGGGGT 
CTCTGGCATC CCTATAACAG GATTGATAGT CGAATAATAG AGGCAGCTCA TCAGGTTGGT 
GAGGATGAGA TAAGCCTGTC GACACTTGGA CGTGTCTATA CTATTGATTT TAATTCTATG 
CAGCAAATAA ATGAGGATAC TGGAACAGCA CGTGCCATTC AGCGGAAACC AAACCCTTTA 
GCCAATACAA ACACTAGTGG ACATTCAGAA TTGAAGAAGG ATGATGCTCG AGCACAGCTA 
ATGAAAGAAG ACCCAGAATT GGCAAAATCC TTTATCAAAA CACTGTTTGG TGTTCTTTAT 
GAAGTGTATA GTTCTTCAGC TGGACCTGCT GTTAGACACA AGTGCCTTAG AGCAATACTT 
AGAATAATTT ATTTTGCTGA TGCTGAACTC CTGAAGGATG TGCTGAAAAA CCATGCTGTG 
TCAAGTCACA TTGCCTCCAT GCTGTCAAGT CAAGACCTTA AGATAGTAGT TGGAGCCCTG 
CAGATGGCAG AAATCTTAAT GCAGAAGCTA CCTGACATTT TTAGTGTTTA CTTCAGAAGA 
GAAGGAGTGA TGCATCAAGT GAAAAACTTA GCGGAGTCTG AGGCTTTGCT AACGAGCCCA 
CCAAAAGTAT GCACTAATGG ATCAGGAACA CTGGGTACCA CTACAACTAT AAGTACTGGA 
ACAGCCACTG CTGCCACTAA TGCAGCTGCA GATTTGGGCT CTCCCAGCTT ACAACACAGC 
CGGGAGGATT CTTTAGATCT GAGCCCACAG GGACGACTGA GTGATGTTCT GAAGAGAAAA 
AGACTGCCAA AACGAGGGCC AAGGAGACCA AAATATTCTC CCCCAAGAGA TGATGACAAA 
GTAGACAATC AAGCAAAAAG CCCCACGACT ACTCAGTCTC CTAAATCTTC TTTCTTAGCA 
AGTTTAAATC CCAAAACATG GGGAAGATTA AGTACACAGT CTAACAGTAA TAACATTGAA 
CCAGCAAGAA CAGCAGGTGT AAGTGGTCTT GCAAGAGCTG CTTCTAAGGA TACAATTTCC 
AATAACAGAG AAAAAATTAA GGGTTGGATT AAGGAGCAAG CCCATAAATT TGTAGACCGT 
TATTTTAGTT CGGAAAACAT GGATGGAAGC AATCCTGCAC TAAATGTATT ACAGAGACTT 
TGCACTGCAA CTGAACAACT TAACCTCCAG GTGGATGGTG GAACAGAGTG CCTTGTAGAA 
ATCCGTAGCA TTGTCTCGGA GTCTGACGTC TCCTCATTTG AAATCCAGCA TAGTGGATTT 
GTGAAACAAC TGCTGCTTTA TTTGACATCT AAAAGTGAGA AAGATGCTGT AAGCAGGGAT 
ATCAGATTGA AAAGATTTCT TCATGTATTT TTCTCCTCTC CACTTCCTGG AGAAGAACCC 
CTTGGAAGAT TAGAGCCATT AGAAAATGCA CCTTTGTTGG CATTAGTCCA TAAAATGAAC 
AATTGCCTCA GTCAGATGGA GCAGTTTCCA GTGAAAGTGC ATGACTTCCC CAGTGGAAAT 
GGAACAGGGA GCAGTTTTTC TCTTAACAGA GGATCCCAAG CTTTAAAATT CTTCAATACG 
CATCAGTTAA AATGCCAGCT GCAAAGACAT CCAGACTGTG CTAATGTGAA ACAGTGGAAG 
GGTGGACCTG TGAAGATTGA TCCTCTGGCT TTGGTTCAAG CCATTGAAAG ATATCTTGTT 
GTTAGAGGTT ATGGAAGAGT TAGAGAAGAT GATGAGGATA GTGATGATGA TGGGTCAGAT 
GAAGAAATAG ATGAATCCTT GGCTGCTCAA TTCTTAAATT CAGGGAATGT GAGACATAGA 
TTGCAGTTTT ACATTGGGGA TCACTTGCTA CCATATAATA TGACTGTATA TCAAGCAGTT 
AAGCTGTACA GTTTGCAAGC TGAGGAGGAG AGGGAATCGA CTGATGATGA AAGCAATCCA 
TTAGGAAGAG CTGGGATTTG GACAAAAACA CATACCATTT GGTACAAACC TGTACGGGAG 
GATGAAGATG GCAACAAAGA CTGTGTTGGT GGTAAAAGAG GAAGAGCTCA AACTGCTCCC 
ACAAAAACCT CACCTAGGAA TTCTAAAAAG CATGATGAGT TGTGGCACGA TGGTGTATGC 
CCTTCAGTAT TGAATCCTTT AGAAGTTTAC CTCATATCTA CTCCTCCTGA AAACATAACC 
TTTGAAGATC CTTCATTAGA TGTTATTCTT CTTTTGAGAG TTTTACATGC TATCAGTCGA 
TACTGGTATT ATTTGTATGA TAATGCAATA TGCAAGGAGA TTATTCCAAC TTCGGAATTT 
ATCAATAGTA AACTGACAGC AAAAGCAAAC AGACAGCTTC AAGATCCACT GGTAATTATG 
ACAGGAAACA TACCGACTTG GCTGACAGAG CTTGGAAAAA CATGCCCATT TTTCTTTCCA 
TTTGATACCC GGCAAATGCT GTTTTATGTA ACTGCTTTTG ATCGTGATCG AGCCATGCAA 
AGACTACTGG ATACTAATCC AGAAATCAAT CAATCAGATT CTCAGGATAG CAGAGTGGCA 
CCACGACTGG ACAGGAAAAA ACGCACTGTG AACAGAGATG AGCTGTTGAA ACAGGCAGAA 
TCTGTGATGC AGGATCTAGG CAGTTCAAGA GCCATGTTGG AAATCCAGTA TGAGAATGAA 
GTTGGCACAG GCCTGGGCCC TACACTAGAG TTCTATGCAC TTGTATCTCA GGAACTACAG 
AGAGCAGACT TGGGCCTTTG GAGGGGAGAA GAAGTAACTT TAGCCAATCC AAAAGGAAGC 
CAAGAAGGTA CCAAGTATAT TCATAATCTC CAAGGCCTTT TTGCACTTCC TTTTGGTAGA 
ACAGCCAAGC CTGCTCACAT TGCAAAAGTT AAAATGAAGT TCCGCTTTCT GGGAAAACTA 
ATGGCTAAGG CAATCATGGA TTTTAGACTG GTGGATCTTC CTCTTGGACT TCCTTTTTAT 
AAATGGATGT TACGCCAGGA AACTTCTTTG ACGTCACATG ACTTGTTCAG TATTGATCCA 
GTAGTAGCCA AATCAATATA TCACCTTGAA GATATTGTAA GACAAAAGAA AAGACTTGAG 
CAGGACAAAA CACAGACCAA AGAAAGTCTA CAGTATGCAT TGGAAACCCT GACTATGAAT 
GGCTGCTCAG TGGAGGATCT AGGGCTGGAT TTCACACTTC CTGGATTTCC TAACATCGAG 
CTGAAAAAAG GGGGAAAAGA TACACCAGTC ACCATCCACA ACTTGGAGGA GTACCTCAGA 
TTGGTTATAT TCTGGGCACT TAACGAAGGT GTTGCCAGAC AGTTTGATTC TTTCAGAGAT 
GGATTTGAAT CAGTCTTCCC CCTCAGTCAT CTTCAATACT TTTATCCTGA GGAGTTGGAG 
CAACTCTTAT GTGGCAGCAA AACAGACACA TGGGATGCCA AGACCTTAAT GGAATGTTGC 
AGGCCAGATC ACGGTTATAC ACATGACAGT CGAGCAGTGA AGTATCTCTT TGAAATTCTC 
AGTAGTTTTG ATAGTGAGCA GCAGAGACTG TTTCTTCAGT TTGTGACCGG TAGTCCCAGG 
CTGCCAGTAG GAGGCTTTCG AAGTTTGAAT CCTCCATTGA CAATTGTACG GAAGACATTT 
GAGTCTACAG AGAATCCAGA TGACTTCTTG CCCTCAGTAA TGACTTGTGT GAACTATCTC 
AAATTGCCGG ACTATTCAAC TATTGAGATA ATGCGTGAAA AACTCTTGAT AGCTGCAAGA 
GAAGGGCAGC AGTCATTCCA TCTTTCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015132458.1
CDS	134..6103
Translation	MSNRPNNNPGGSLRRSQRNTAGAQPQDDAAGGRSHLGQAKHKAHSPPESRKSISKTPKVQSNTTSEQSKGHFSKRGCSSSAVLIPQEDSERVSTSEKQKTGQVPKKDNSRGVKRSASPDYSRTNSPSSAKKPKALQHAETSSESNKPHTKSKRRHLDQEQQKSTQLPSTSKAHTRKGGAAGSSRSQKRKRTESTSCIKSRSAVESTGAEEKSAKSSKLASKSVTSAKAGCSTITDSSSSASTSSSSSAVASASSTVPQGARVKQGLRKSTKKRSESPPAELPSLRRSTRQKTTGSCASTSRRGSGLGKRGAAEARRQEKMADPDNNQDGVNSSAARTDETPQGAAASSSVAGAVGMTTSGESESDDSEMGRLQALLEARGLPPHLFGPLGPRMSQLFHRTIGSGASSKAQQLLQGLQATDESQQLQAVIEMCQLLVMGNEETLGGFPVKSVVPALITLLQMEHNFDIMNHACRALTYMMEALPRSSAVVVDAIPVFLEKLQVIQCIDVAEQALTALEMLSRRHSKAILQAGGLADCLLYLEFFSINAQRNALAIAANCCQSITPDEFHFVADSLPLLTQRLTHQDKKSVESTCLCFARLVDNFQHEENLLQQVASKDLLTNIQQLLVVTPPILSSGMFIMVVRMFSLMCSNCPTLAVQLMKQNIAETLHFLLCGASNGSCQEQIDLVPRSPQELYELTSLICELMPCLPKEGIFAVDTMLKKGNAQNTDGAIWQWRDDRGLWHPYNRIDSRIIEAAHQVGEDEISLSTLGRVYTIDFNSMQQINEDTGTARAIQRKPNPLANTNTSGHSELKKDDARAQLMKEDPELAKSFIKTLFGVLYEVYSSSAGPAVRHKCLRAILRIIYFADAELLKDVLKNHAVSSHIASMLSSQDLKIVVGALQMAEILMQKLPDIFSVYFRREGVMHQVKNLAESEALLTSPPKVCTNGSGTLGTTTTISTGTATAATNAAADLGSPSLQHSREDSLDLSPQGRLSDVLKRKRLPKRGPRRPKYSPPRDDDKVDNQAKSPTTTQSPKSSFLASLNPKTWGRLSTQSNSNNIEPARTAGVSGLARAASKDTISNNREKIKGWIKEQAHKFVDRYFSSENMDGSNPALNVLQRLCTATEQLNLQVDGGTECLVEIRSIVSESDVSSFEIQHSGFVKQLLLYLTSKSEKDAVSRDIRLKRFLHVFFSSPLPGEEPLGRLEPLENAPLLALVHKMNNCLSQMEQFPVKVHDFPSGNGTGSSFSLNRGSQALKFFNTHQLKCQLQRHPDCANVKQWKGGPVKIDPLALVQAIERYLVVRGYGRVREDDEDSDDDGSDEEIDESLAAQFLNSGNVRHRLQFYIGDHLLPYNMTVYQAVKLYSLQAEEERESTDDESNPLGRAGIWTKTHTIWYKPVREDEDGNKDCVGGKRGRAQTAPTKTSPRNSKKHDELWHDGVCPSVLNPLEVYLISTPPENITFEDPSLDVILLLRVLHAISRYWYYLYDNAICKEIIPTSEFINSKLTAKANRQLQDPLVIMTGNIPTWLTELGKTCPFFFPFDTRQMLFYVTAFDRDRAMQRLLDTNPEINQSDSQDSRVAPRLDRKKRTVNRDELLKQAESVMQDLGSSRAMLEIQYENEVGTGLGPTLEFYALVSQELQRADLGLWRGEEVTLANPKGSQEGTKYIHNLQGLFALPFGRTAKPAHIAKVKMKFRFLGKLMAKAIMDFRLVDLPLGLPFYKWMLRQETSLTSHDLFSIDPVVAKSIYHLEDIVRQKKRLEQDKTQTKESLQYALETLTMNGCSVEDLGLDFTLPGFPNIELKKGGKDTPVTIHNLEEYLRLVIFWALNEGVARQFDSFRDGFESVFPLSHLQYFYPEELEQLLCGSKTDTWDAKTLMECCRPDHGYTHDSRAVKYLFEILSSFDSEQQRLFLQFVTGSPRLPVGGFRSLNPPLTIVRKTFESTENPDDFLPSVMTCVNYLKLPDYSTIEIMREKLLIAAREGQQSFHLS

Target ORF information:

RefSeq Version	XM_015276972.2
Organism	Gallus gallus(chicken)
Definition	Gallus gallus thyroid hormone receptor interactor 12 (TRIP12), transcript variant X12, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015276972.2

ORF Insert Sequence:

ATGTCCAACC GGCCTAATAA CAATCCAGGG GGGTCACTGC GACGTTCACA GAGGAACACT 
GCTGGGGCCC AGCCACAAGA CGACGCAGCA GGAGGAAGGT CACATTTAGG GCAGGCAAAA 
CATAAGGCAC ATAGCCCTCC TGAGAGTAGA AAATCTATTT CAAAGACACC CAAAGTGCAG 
TCTAATACTA CTTCTGAGCA GTCCAAGGGA CACTTTTCTA AAAGAGGCTG TAGTTCATCT 
GCTGTTTTAA TACCACAAGA AGATTCAGAA AGAGTCAGTA CTTCAGAAAA GCAAAAAACG 
GGGCAAGTGC CTAAGAAAGA CAATTCTCGA GGAGTTAAAC GCAGTGCTAG TCCAGATTAC 
AGCAGGACCA ATTCTCCTAG CTCTGCTAAA AAACCTAAAG CACTTCAACA CGCCGAAACT 
TCCTCGGAAA GTAACAAGCC ACATACTAAA TCCAAGAGAA GACACTTAGA CCAAGAACAG 
CAGAAGTCTA CACAATTGCC ATCAACAAGC AAGGCTCACA CCAGAAAGGG TGGAGCTGCT 
GGTAGCTCCC GAAGTCAGAA AAGGAAAAGG ACAGAGAGTA CGTCTTGTAT AAAGAGTCGT 
TCAGCAGTTG AATCAACTGG AGCTGAAGAG AAGTCAGCAA AATCCTCCAA GCTGGCTTCA 
AAATCGGTGA CCTCAGCCAA AGCTGGGTGT AGCACCATCA CTGATTCTTC TTCTTCAGCT 
TCTACATCCT CTTCCTCTTC TGCTGTTGCC TCTGCTTCTT CCACTGTTCC TCAGGGTGCC 
AGAGTGAAAC AGGGCCTAAG AAAATCCACA AAGAAGCGCA GTGAATCACC ACCTGCTGAG 
CTCCCCAGTT TACGGCGGAG CACACGGCAA AAGACCACGG GCTCCTGTGC GAGTACCAGT 
CGGCGGGGCT CTGGCCTGGG CAAAAGAGGA GCAGCTGAAG CTCGCCGACA GGAGAAGATG 
GCTGATCCTG ACAACAACCA GGATGGAGTT AACTCTTCAG CTGCACGTAC AGATGAGACT 
CCCCAGGGAG CTGCAGCTTC TAGCTCTGTT GCTGGGGCTG TAGGTATGAC AACATCTGGA 
GAAAGTGAGT CAGATGACTC TGAGATGGGA AGACTACAAG CTCTGTTAGA GGCTAGGGGT 
CTTCCTCCTC ACCTGTTTGG CCCTCTTGGT CCTCGGATGT CGCAGCTCTT CCACAGGACA 
ATTGGAAGTG GAGCTAGTTC TAAAGCCCAG CAGCTTTTAC AAGGTCTCCA AGCCACTGAT 
GAAAGTCAGC AACTACAGGC AGTTATTGAG ATGTGCCAGC TGTTGGTCAT GGGAAATGAA 
GAAACACTGG GAGGATTTCC AGTCAAGAGT GTTGTGCCAG CTTTGATAAC ATTGCTGCAG 
ATGGAGCATA ACTTTGACAT CATGAACCAT GCTTGTCGAG CCTTAACATA CATGATGGAA 
GCACTTCCCA GATCATCTGC TGTAGTGGTA GATGCAATTC CTGTCTTCTT GGAAAAGCTG 
CAAGTTATTC AGTGCATTGA TGTGGCAGAG CAGGCACTGA CAGCCCTAGA GATGCTATCA 
CGCAGGCATA GTAAAGCCAT TCTGCAGGCA GGTGGGTTGG CAGACTGCTT GCTGTATCTG 
GAATTCTTCA GTATAAATGC GCAGAGGAAT GCACTAGCTA TTGCTGCCAA TTGCTGCCAG 
AGTATAACAC CTGATGAGTT TCACTTTGTG GCAGACTCTT TGCCACTGCT TACACAAAGG 
TTAACCCATC AGGACAAGAA GTCTGTTGAA AGCACTTGTC TCTGTTTTGC ACGGCTGGTA 
GACAATTTCC AGCATGAGGA GAACTTGCTG CAGCAGGTTG CTTCCAAGGA CTTGTTAACA 
AACATCCAGC AACTCTTGGT AGTGACACCT CCTATCCTGA GCTCAGGAAT GTTCATCATG 
GTGGTGCGCA TGTTTTCCTT AATGTGTTCC AATTGTCCAA CGCTTGCAGT CCAACTTATG 
AAGCAAAACA TTGCAGAAAC ACTTCACTTT CTCCTTTGTG GAGCCTCAAA TGGGAGTTGT 
CAAGAACAAA TTGACCTTGT TCCACGAAGT CCTCAAGAAC TCTATGAGCT TACTTCTCTT 
ATCTGTGAAC TGATGCCTTG TCTGCCAAAA GAGGGGATAT TTGCTGTTGA TACTATGCTA 
AAGAAAGGAA ATGCACAAAA TACAGATGGT GCGATATGGC AATGGCGAGA TGACAGGGGT 
CTCTGGCATC CCTATAACAG GATTGATAGT CGAATAATAG AGGCAGCTCA TCAGGTTGGT 
GAGGATGAGA TAAGCCTGTC GACACTTGGA CGTGTCTATA CTATTGATTT TAATTCTATG 
CAGCAAATAA ATGAGGATAC TGGAACAGCA CGTGCCATTC AGCGGAAACC AAACCCTTTA 
GCCAATACAA ACACTAGTGG ACATTCAGAA TTGAAGAAGG ATGATGCTCG AGCACAGCTA 
ATGAAAGAAG ACCCAGAATT GGCAAAATCC TTTATCAAAA CACTGTTTGG TGTTCTTTAT 
GAAGTGTATA GTTCTTCAGC TGGACCTGCT GTTAGACACA AGTGCCTTAG AGCAATACTT 
AGAATAATTT ATTTTGCTGA TGCTGAACTC CTGAAGGATG TGCTGAAAAA CCATGCTGTG 
TCAAGTCACA TTGCCTCCAT GCTGTCAAGT CAAGACCTTA AGATAGTAGT TGGAGCCCTG 
CAGATGGCAG AAATCTTAAT GCAGAAGCTA CCTGACATTT TTAGTGTTTA CTTCAGAAGA 
GAAGGAGTGA TGCATCAAGT GAAAAACTTA GCGGAGTCTG AGGCTTTGCT AACGAGCCCA 
CCAAAAGTAT GCACTAATGG ATCAGGAACA CTGGGTACCA CTACAACTAT AAGTACTGGA 
ACAGCCACTG CTGCCACTAA TGCAGCTGCA GATTTGGGCT CTCCCAGCTT ACAACACAGC 
CGGGAGGATT CTTTAGATCT GAGCCCACAG GGACGACTGA GTGATGTTCT GAAGAGAAAA 
AGACTGCCAA AACGAGGGCC AAGGAGACCA AAATATTCTC CCCCAAGAGA TGATGACAAA 
GTAGACAATC AAGCAAAAAG CCCCACGACT ACTCAGTCTC CTAAATCTTC TTTCTTAGCA 
AGTTTAAATC CCAAAACATG GGGAAGATTA AGTACACAGT CTAACAGTAA TAACATTGAA 
CCAGCAAGAA CAGCAGGTGT AAGTGGTCTT GCAAGAGCTG CTTCTAAGGA TACAATTTCC 
AATAACAGAG AAAAAATTAA GGGTTGGATT AAGGAGCAAG CCCATAAATT TGTAGACCGT 
TATTTTAGTT CGGAAAACAT GGATGGAAGC AATCCTGCAC TAAATGTATT ACAGAGACTT 
TGCACTGCAA CTGAACAACT TAACCTCCAG GTGGATGGTG GAACAGAGTG CCTTGTAGAA 
ATCCGTAGCA TTGTCTCGGA GTCTGACGTC TCCTCATTTG AAATCCAGCA TAGTGGATTT 
GTGAAACAAC TGCTGCTTTA TTTGACATCT AAAAGTGAGA AAGATGCTGT AAGCAGGGAT 
ATCAGATTGA AAAGATTTCT TCATGTATTT TTCTCCTCTC CACTTCCTGG AGAAGAACCC 
CTTGGAAGAT TAGAGCCATT AGAAAATGCA CCTTTGTTGG CATTAGTCCA TAAAATGAAC 
AATTGCCTCA GTCAGATGGA GCAGTTTCCA GTGAAAGTGC ATGACTTCCC CAGTGGAAAT 
GGAACAGGGA GCAGTTTTTC TCTTAACAGA GGATCCCAAG CTTTAAAATT CTTCAATACG 
CATCAGTTAA AATGCCAGCT GCAAAGACAT CCAGACTGTG CTAATGTGAA ACAGTGGAAG 
GGTGGACCTG TGAAGATTGA TCCTCTGGCT TTGGTTCAAG CCATTGAAAG ATATCTTGTT 
GTTAGAGGTT ATGGAAGAGT TAGAGAAGAT GATGAGGATA GTGATGATGA TGGGTCAGAT 
GAAGAAATAG ATGAATCCTT GGCTGCTCAA TTCTTAAATT CAGGGAATGT GAGACATAGA 
TTGCAGTTTT ACATTGGGGA TCACTTGCTA CCATATAATA TGACTGTATA TCAAGCAGTT 
AAGCTGTACA GTTTGCAAGC TGAGGAGGAG AGGGAATCGA CTGATGATGA AAGCAATCCA 
TTAGGAAGAG CTGGGATTTG GACAAAAACA CATACCATTT GGTACAAACC TGTACGGGAG 
GATGAAGATG GCAACAAAGA CTGTGTTGGT GGTAAAAGAG GAAGAGCTCA AACTGCTCCC 
ACAAAAACCT CACCTAGGAA TTCTAAAAAG CATGATGAGT TGTGGCACGA TGGTGTATGC 
CCTTCAGTAT TGAATCCTTT AGAAGTTTAC CTCATATCTA CTCCTCCTGA AAACATAACC 
TTTGAAGATC CTTCATTAGA TGTTATTCTT CTTTTGAGAG TTTTACATGC TATCAGTCGA 
TACTGGTATT ATTTGTATGA TAATGCAATA TGCAAGGAGA TTATTCCAAC TTCGGAATTT 
ATCAATAGTA AACTGACAGC AAAAGCAAAC AGACAGCTTC AAGATCCACT GGTAATTATG 
ACAGGAAACA TACCGACTTG GCTGACAGAG CTTGGAAAAA CATGCCCATT TTTCTTTCCA 
TTTGATACCC GGCAAATGCT GTTTTATGTA ACTGCTTTTG ATCGTGATCG AGCCATGCAA 
AGACTACTGG ATACTAATCC AGAAATCAAT CAATCAGATT CTCAGGATAG CAGAGTGGCA 
CCACGACTGG ACAGGAAAAA ACGCACTGTG AACAGAGATG AGCTGTTGAA ACAGGCAGAA 
TCTGTGATGC AGGATCTAGG CAGTTCAAGA GCCATGTTGG AAATCCAGTA TGAGAATGAA 
GTTGGCACAG GCCTGGGCCC TACACTAGAG TTCTATGCAC TTGTATCTCA GGAACTACAG 
AGAGCAGACT TGGGCCTTTG GAGGGGAGAA GAAGTAACTT TAGCCAATCC AAAAGGAAGC 
CAAGAAGGTA CCAAGTATAT TCATAATCTC CAAGGCCTTT TTGCACTTCC TTTTGGTAGA 
ACAGCCAAGC CTGCTCACAT TGCAAAAGTT AAAATGAAGT TCCGCTTTCT GGGAAAACTA 
ATGGCTAAGG CAATCATGGA TTTTAGACTG GTGGATCTTC CTCTTGGACT TCCTTTTTAT 
AAATGGATGT TACGCCAGGA AACTTCTTTG ACGTCACATG ACTTGTTCAG TATTGATCCA 
GTAGTAGCCA AATCAATATA TCACCTTGAA GATATTGTAA GACAAAAGAA AAGACTTGAG 
CAGGACAAAA CACAGACCAA AGAAAGTCTA CAGTATGCAT TGGAAACCCT GACTATGAAT 
GGCTGCTCAG TGGAGGATCT AGGGCTGGAT TTCACACTTC CTGGATTTCC TAACATCGAG 
CTGAAAAAAG GGGGAAAAGA TACACCAGTC ACCATCCACA ACTTGGAGGA GTACCTCAGA 
TTGGTTATAT TCTGGGCACT TAACGAAGGT GTTGCCAGAC AGTTTGATTC TTTCAGAGAT 
GGATTTGAAT CAGTCTTCCC CCTCAGTCAT CTTCAATACT TTTATCCTGA GGAGTTGGAG 
CAACTCTTAT GTGGCAGCAA AACAGACACA TGGGATGCCA AGACCTTAAT GGAATGTTGC 
AGGCCAGATC ACGGTTATAC ACATGACAGT CGAGCAGTGA AGTATCTCTT TGAAATTCTC 
AGTAGTTTTG ATAGTGAGCA GCAGAGACTG TTTCTTCAGT TTGTGACCGG TAGTCCCAGG 
CTGCCAGTAG GAGGCTTTCG AAGTTTGAAT CCTCCATTGA CAATTGTACG GAAGACATTT 
GAGTCTACAG AGAATCCAGA TGACTTCTTG CCCTCAGTAA TGACTTGTGT GAACTATCTC 
AAATTGCCGG ACTATTCAAC TATTGAGATA ATGCGTGAAA AACTCTTGAT AGCTGCAAGA 
GAAGGGCAGC AGTCATTCCA TCTTTCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TRIP12 ( XM_025153691.1 ) cDNA ORF clone, Gallus gallus(chicken) -> XP_025009459.1 Gallus gallus thyroid hormone receptor interactor 12 (TRIP12), transcript variant X10, mRNA.

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CloneID	OGa152841
Clone ID Related Accession (Same CDS sequence)	XM_025153691.1
Accession Version	XM_025153691.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6057bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-16
Organism	Gallus gallus(chicken)
Product	E3 ubiquitin-protein ligase TRIP12 isoform X6
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006096.5) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Gallus gallus Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCCAACC GGCCTAATAA CAATCCAGGG GGGTCACTGC GACGTTCACA GAGGAACACT 
GCTGGGGCCC AGCCACAAGA CGACGCAGCA GGAGGAAGAG GCTGTAGTTC ATCTGCTGTT 
TTAATACCAC AAGAAGATTC AGAAAGAGTC AGTACTTCAG AAAAGCAAAA AACGGGGCAA 
GTGCCTAAGA AAGACAATTC TCGAGGAGTT AAACGCAGTG CTAGTCCAGA TTACAGCAGG 
ACCAATTCTC CTAGCTCTGC TAAAAAACCT AAAGCACTTC AACACGCCGA AACTTCCTCG 
GAAAGTAACA AGCCACATAC TAAATCCAAG AGAAGACACT TAGACCAAGA ACAGCAGAAG 
TCTACACAAT TGCCATCAAC AAGCAAGGCT CACACCAGAA AGGGTGGAGC TGCTGGTAGC 
TCCCGAAGTC AGAAAAGGAA AAGGACAGAG AGTACGTCTT GTATAAAGAG TCGTTCAGCA 
GTTGAATCAA CTGGAGCTGA AGAGAAGTCA GCAAAATCCT CCAAGCTGGC TTCAAAATCG 
GTGACCTCAG CCAAAGCTGG GTGTAGCACC ATCACTGATT CTTCTTCTTC AGCTTCTACA 
TCCTCTTCCT CTTCTGCTGT TGCCTCTGCT TCTTCCACTG TTCCTCAGGG TGCCAGAGTG 
AAACAGGGTA AGGACCAGAA TAAGGCTAGA CGTTCACGTT CTGCGTCCAG CCCTAGTCCA 
AGAAGGAGCA GCAGGGACAA AGAACAGAGC AAAACAGGTG GCTCTTCAAA GTTTGACTGG 
GCTGCTCGAT TCAGTCCAAA AGTTAGTCTC CCTAAAACAA AACTGTCTCT ACCAGGCTCT 
TCTAAGTCAG AGACCTCAAA ACCTGGACCT TCAGGACTAC AGGCTAAGCT AGCAAGCCTA 
AGAAAATCCA CAAAGAAGCG CAGTGAATCA CCACCTGCTG AGCTCCCCAG TTTACGGCGG 
AGCACACGGC AAAAGACCAC GGGCTCCTGT GCGAGTACCA GTCGGCGGGG CTCTGGCCTG 
GGCAAAAGAG GAGCAGCTGA AGCTCGCCGA CAGGAGAAGA TGGCTGATCC TGACAACAAC 
CAGGATGGAG TTAACTCTTC AGCTGCACGT ACAGATGAGA CTCCCCAGGG AGCTGCAGCT 
TCTAGCTCTG TTGCTGGGGC TGTAGGTATG ACAACATCTG GAGAAAGTGA GTCAGATGAC 
TCTGAGATGG GAAGACTACA AGCTCTGTTA GAGGCTAGGG GTCTTCCTCC TCACCTGTTT 
GGCCCTCTTG GTCCTCGGAT GTCGCAGCTC TTCCACAGGA CAATTGGAAG TGGAGCTAGT 
TCTAAAGCCC AGCAGCTTTT ACAAGGTCTC CAAGCCACTG ATGAAAGTCA GCAACTACAG 
GCAGTTATTG AGATGTGCCA GCTGTTGGTC ATGGGAAATG AAGAAACACT GGGAGGATTT 
CCAGTCAAGA GTGTTGTGCC AGCTTTGATA ACATTGCTGC AGATGGAGCA TAACTTTGAC 
ATCATGAACC ATGCTTGTCG AGCCTTAACA TACATGATGG AAGCACTTCC CAGATCATCT 
GCTGTAGTGG TAGATGCAAT TCCTGTCTTC TTGGAAAAGC TGCAAGTTAT TCAGTGCATT 
GATGTGGCAG AGCAGGCACT GACAGCCCTA GAGATGCTAT CACGCAGGCA TAGTAAAGCC 
ATTCTGCAGG CAGGTGGGTT GGCAGACTGC TTGCTGTATC TGGAATTCTT CAGTATAAAT 
GCGCAGAGGA ATGCACTAGC TATTGCTGCC AATTGCTGCC AGAGTATAAC ACCTGATGAG 
TTTCACTTTG TGGCAGACTC TTTGCCACTG CTTACACAAA GGTTAACCCA TCAGGACAAG 
AAGTCTGTTG AAAGCACTTG TCTCTGTTTT GCACGGCTGG TAGACAATTT CCAGCATGAG 
GAGAACTTGC TGCAGCAGGT TGCTTCCAAG GACTTGTTAA CAAACATCCA GCAACTCTTG 
GTAGTGACAC CTCCTATCCT GAGCTCAGGA ATGTTCATCA TGGTGGTGCG CATGTTTTCC 
TTAATGTGTT CCAATTGTCC AACGCTTGCA GTCCAACTTA TGAAGCAAAA CATTGCAGAA 
ACACTTCACT TTCTCCTTTG TGGAGCCTCA AATGGGAGTT GTCAAGAACA AATTGACCTT 
GTTCCACGAA GTCCTCAAGA ACTCTATGAG CTTACTTCTC TTATCTGTGA ACTGATGCCT 
TGTCTGCCAA AAGAGGGGAT ATTTGCTGTT GATACTATGC TAAAGAAAGG AAATGCACAA 
AATACAGATG GTGCGATATG GCAATGGCGA GATGACAGGG GTCTCTGGCA TCCCTATAAC 
AGGATTGATA GTCGAATAAT AGAGGCAGCT CATCAGGTTG GTGAGGATGA GATAAGCCTG 
TCGACACTTG GACGTGTCTA TACTATTGAT TTTAATTCTA TGCAGCAAAT AAATGAGGAT 
ACTGGAACAG CACGTGCCAT TCAGCGGAAA CCAAACCCTT TAGCCAATAC AAACACTAGT 
GGACATTCAG AATTGAAGAA GGATGATGCT CGAGCACAGC TAATGAAAGA AGACCCAGAA 
TTGGCAAAAT CCTTTATCAA AACACTGTTT GGTGTTCTTT ATGAAGTGTA TAGTTCTTCA 
GCTGGACCTG CTGTTAGACA CAAGTGCCTT AGAGCAATAC TTAGAATAAT TTATTTTGCT 
GATGCTGAAC TCCTGAAGGA TGTGCTGAAA AACCATGCTG TGTCAAGTCA CATTGCCTCC 
ATGCTGTCAA GTCAAGACCT TAAGATAGTA GTTGGAGCCC TGCAGATGGC AGAAATCTTA 
ATGCAGAAGC TACCTGACAT TTTTAGTGTT TACTTCAGAA GAGAAGGAGT GATGCATCAA 
GTGAAAAACT TAGCGGAGTC TGAGGCTTTG CTAACGAGCC CACCAAAAGT ATGCACTAAT 
GGATCAGGAA CACTGGGTAC CACTACAACT ATAAGTACTG GAACAGCCAC TGCTGCCACT 
AATGCAGCTG CAGATTTGGG CTCTCCCAGC TTACAACACA GCCGGGAGGA TTCTTTAGAT 
CTGAGCCCAC AGGGACGACT GAGTGATGTT CTGAAGAGAA AAAGACTGCC AAAACGAGGG 
CCAAGGAGAC CAAAATATTC TCCCCCAAGA GATGATGACA AAGTAGACAA TCAAGCAAAA 
AGCCCCACGA CTACTCAGTC TCCTAAATCT TCTTTCTTAG CAAGTTTAAA TCCCAAAACA 
TGGGGAAGAT TAAGTACACA GTCTAACAGT AATAACATTG AACCAGCAAG AACAGCAGGT 
GTAAGTGGTC TTGCAAGAGC TGCTTCTAAG GATACAATTT CCAATAACAG AGAAAAAATT 
AAGGGTTGGA TTAAGGAGCA AGCCCATAAA TTTGTAGACC GTTATTTTAG TTCGGAAAAC 
ATGGATGGAA GCAATCCTGC ACTAAATGTA TTACAGAGAC TTTGCACTGC AACTGAACAA 
CTTAACCTCC AGGTGGATGG TGGAACAGAG TGCCTTGTAG AAATCCGTAG CATTGTCTCG 
GAGTCTGACG TCTCCTCATT TGAAATCCAG CATAGTGGAT TTGTGAAACA ACTGCTGCTT 
TATTTGACAT CTAAAAGTGA GAAAGATGCT GTAAGCAGGG ATATCAGATT GAAAAGATTT 
CTTCATGTAT TTTTCTCCTC TCCACTTCCT GGAGAAGAAC CCCTTGGAAG ATTAGAGCCA 
TTAGAAAATG CACCTTTGTT GGCATTAGTC CATAAAATGA ACAATTGCCT CAGTCAGATG 
GAGCAGTTTC CAGTGAAAGT GCATGACTTC CCCAGTGGAA ATGGAACAGG GAGCAGAGGA 
TCCCAAGCTT TAAAATTCTT CAATACGCAT CAGTTAAAAT GCCAGCTGCA AAGACATCCA 
GACTGTGCTA ATGTGAAACA GTGGAAGGGT GGACCTGTGA AGATTGATCC TCTGGCTTTG 
GTTCAAGCCA TTGAAAGATA TCTTGTTGTT AGAGGTTATG GAAGAGTTAG AGAAGATGAT 
GAGGATAGTG ATGATGATGG GTCAGATGAA GAAATAGATG AATCCTTGGC TGCTCAATTC 
TTAAATTCAG GGAATGTGAG ACATAGATTG CAGTTTTACA TTGGGGATCA CTTGCTACCA 
TATAATATGA CTGTATATCA AGCAGTTAAG CTGTACAGTT TGCAAGCTGA GGAGGAGAGG 
GAATCGACTG ATGATGAAAG CAATCCATTA GGAAGAGCTG GGATTTGGAC AAAAACACAT 
ACCATTTGGT ACAAACCTGT ACGGGAGGAT GAAGATGGCA ACAAAGACTG TGTTGGTGGT 
AAAAGAGGAA GAGCTCAAAC TGCTCCCACA AAAACCTCAC CTAGGAATTC TAAAAAGCAT 
GATGAGTTGT GGCACGATGG TGTATGCCCT TCAGTATTGA ATCCTTTAGA AGTTTACCTC 
ATATCTACTC CTCCTGAAAA CATAACCTTT GAAGATCCTT CATTAGATGT TATTCTTCTT 
TTGAGAGTTT TACATGCTAT CAGTCGATAC TGGTATTATT TGTATGATAA TGCAATATGC 
AAGGAGATTA TTCCAACTTC GGAATTTATC AATAGTAAAC TGACAGCAAA AGCAAACAGA 
CAGCTTCAAG ATCCACTGGT AATTATGACA GGAAACATAC CGACTTGGCT GACAGAGCTT 
GGAAAAACAT GCCCATTTTT CTTTCCATTT GATACCCGGC AAATGCTGTT TTATGTAACT 
GCTTTTGATC GTGATCGAGC CATGCAAAGA CTACTGGATA CTAATCCAGA AATCAATCAA 
TCAGATTCTC AGGATAGCAG AGTGGCACCA CGACTGGACA GGAAAAAACG CACTGTGAAC 
AGAGATGAGC TGTTGAAACA GGCAGAATCT GTGATGCAGG ATCTAGGCAG TTCAAGAGCC 
ATGTTGGAAA TCCAGTATGA GAATGAAGTT GGCACAGGCC TGGGCCCTAC ACTAGAGTTC 
TATGCACTTG TATCTCAGGA ACTACAGAGA GCAGACTTGG GCCTTTGGAG GGGAGAAGAA 
GTAACTTTAG CCAATCCAAA AGGAAGCCAA GAAGGTACCA AGTATATTCA TAATCTCCAA 
GGCCTTTTTG CACTTCCTTT TGGTAGAACA GCCAAGCCTG CTCACATTGC AAAAGTTAAA 
ATGAAGTTCC GCTTTCTGGG AAAACTAATG GCTAAGGCAA TCATGGATTT TAGACTGGTG 
GATCTTCCTC TTGGACTTCC TTTTTATAAA TGGATGTTAC GCCAGGAAAC TTCTTTGACG 
TCACATGACT TGTTCAGTAT TGATCCAGTA GTAGCCAAAT CAATATATCA CCTTGAAGAT 
ATTGTAAGAC AAAAGAAAAG ACTTGAGCAG GACAAAACAC AGACCAAAGA AAGTCTACAG 
TATGCATTGG AAACCCTGAC TATGAATGGC TGCTCAGTGG AGGATCTAGG GCTGGATTTC 
ACACTTCCTG GATTTCCTAA CATCGAGCTG AAAAAAGGGG GAAAAGATAC ACCAGTCACC 
ATCCACAACT TGGAGGAGTA CCTCAGATTG GTTATATTCT GGGCACTTAA CGAAGGTGTT 
GCCAGACAGT TTGATTCTTT CAGAGATGGA TTTGAATCAG TCTTCCCCCT CAGTCATCTT 
CAATACTTTT ATCCTGAGGA GTTGGAGCAA CTCTTATGTG GCAGCAAAAC AGACACATGG 
GATGCCAAGA CCTTAATGGA ATGTTGCAGG CCAGATCACG GTTATACACA TGACAGTCGA 
GCAGTGAAGT ATCTCTTTGA AATTCTCAGT AGTTTTGATA GTGAGCAGCA GAGACTGTTT 
CTTCAGTTTG TGACCGGTAG TCCCAGGCTG CCAGTAGGAG GCTTTCGAAG TTTGAATCCT 
CCATTGACAA TTGTACGGAA GACATTTGAG TCTACAGAGA ATCCAGATGA CTTCTTGCCC 
TCAGTAATGA CTTGTGTGAA CTATCTCAAA TTGCCGGACT ATTCAACTAT TGAGATAATG 
CGTGAAAAAC TCTTGATAGC TGCAAGAGAA GGGCAGCAGT CATTCCATCT TTCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_025009459.1
CDS	138..6194
Translation	MSNRPNNNPGGSLRRSQRNTAGAQPQDDAAGGRGCSSSAVLIPQEDSERVSTSEKQKTGQVPKKDNSRGVKRSASPDYSRTNSPSSAKKPKALQHAETSSESNKPHTKSKRRHLDQEQQKSTQLPSTSKAHTRKGGAAGSSRSQKRKRTESTSCIKSRSAVESTGAEEKSAKSSKLASKSVTSAKAGCSTITDSSSSASTSSSSSAVASASSTVPQGARVKQGKDQNKARRSRSASSPSPRRSSRDKEQSKTGGSSKFDWAARFSPKVSLPKTKLSLPGSSKSETSKPGPSGLQAKLASLRKSTKKRSESPPAELPSLRRSTRQKTTGSCASTSRRGSGLGKRGAAEARRQEKMADPDNNQDGVNSSAARTDETPQGAAASSSVAGAVGMTTSGESESDDSEMGRLQALLEARGLPPHLFGPLGPRMSQLFHRTIGSGASSKAQQLLQGLQATDESQQLQAVIEMCQLLVMGNEETLGGFPVKSVVPALITLLQMEHNFDIMNHACRALTYMMEALPRSSAVVVDAIPVFLEKLQVIQCIDVAEQALTALEMLSRRHSKAILQAGGLADCLLYLEFFSINAQRNALAIAANCCQSITPDEFHFVADSLPLLTQRLTHQDKKSVESTCLCFARLVDNFQHEENLLQQVASKDLLTNIQQLLVVTPPILSSGMFIMVVRMFSLMCSNCPTLAVQLMKQNIAETLHFLLCGASNGSCQEQIDLVPRSPQELYELTSLICELMPCLPKEGIFAVDTMLKKGNAQNTDGAIWQWRDDRGLWHPYNRIDSRIIEAAHQVGEDEISLSTLGRVYTIDFNSMQQINEDTGTARAIQRKPNPLANTNTSGHSELKKDDARAQLMKEDPELAKSFIKTLFGVLYEVYSSSAGPAVRHKCLRAILRIIYFADAELLKDVLKNHAVSSHIASMLSSQDLKIVVGALQMAEILMQKLPDIFSVYFRREGVMHQVKNLAESEALLTSPPKVCTNGSGTLGTTTTISTGTATAATNAAADLGSPSLQHSREDSLDLSPQGRLSDVLKRKRLPKRGPRRPKYSPPRDDDKVDNQAKSPTTTQSPKSSFLASLNPKTWGRLSTQSNSNNIEPARTAGVSGLARAASKDTISNNREKIKGWIKEQAHKFVDRYFSSENMDGSNPALNVLQRLCTATEQLNLQVDGGTECLVEIRSIVSESDVSSFEIQHSGFVKQLLLYLTSKSEKDAVSRDIRLKRFLHVFFSSPLPGEEPLGRLEPLENAPLLALVHKMNNCLSQMEQFPVKVHDFPSGNGTGSRGSQALKFFNTHQLKCQLQRHPDCANVKQWKGGPVKIDPLALVQAIERYLVVRGYGRVREDDEDSDDDGSDEEIDESLAAQFLNSGNVRHRLQFYIGDHLLPYNMTVYQAVKLYSLQAEEERESTDDESNPLGRAGIWTKTHTIWYKPVREDEDGNKDCVGGKRGRAQTAPTKTSPRNSKKHDELWHDGVCPSVLNPLEVYLISTPPENITFEDPSLDVILLLRVLHAISRYWYYLYDNAICKEIIPTSEFINSKLTAKANRQLQDPLVIMTGNIPTWLTELGKTCPFFFPFDTRQMLFYVTAFDRDRAMQRLLDTNPEINQSDSQDSRVAPRLDRKKRTVNRDELLKQAESVMQDLGSSRAMLEIQYENEVGTGLGPTLEFYALVSQELQRADLGLWRGEEVTLANPKGSQEGTKYIHNLQGLFALPFGRTAKPAHIAKVKMKFRFLGKLMAKAIMDFRLVDLPLGLPFYKWMLRQETSLTSHDLFSIDPVVAKSIYHLEDIVRQKKRLEQDKTQTKESLQYALETLTMNGCSVEDLGLDFTLPGFPNIELKKGGKDTPVTIHNLEEYLRLVIFWALNEGVARQFDSFRDGFESVFPLSHLQYFYPEELEQLLCGSKTDTWDAKTLMECCRPDHGYTHDSRAVKYLFEILSSFDSEQQRLFLQFVTGSPRLPVGGFRSLNPPLTIVRKTFESTENPDDFLPSVMTCVNYLKLPDYSTIEIMREKLLIAAREGQQSFHLS

Target ORF information:

RefSeq Version	XM_025153691.1
Organism	Gallus gallus(chicken)
Definition	Gallus gallus thyroid hormone receptor interactor 12 (TRIP12), transcript variant X10, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_025153691.1

ORF Insert Sequence:

ATGTCCAACC GGCCTAATAA CAATCCAGGG GGGTCACTGC GACGTTCACA GAGGAACACT 
GCTGGGGCCC AGCCACAAGA CGACGCAGCA GGAGGAAGAG GCTGTAGTTC ATCTGCTGTT 
TTAATACCAC AAGAAGATTC AGAAAGAGTC AGTACTTCAG AAAAGCAAAA AACGGGGCAA 
GTGCCTAAGA AAGACAATTC TCGAGGAGTT AAACGCAGTG CTAGTCCAGA TTACAGCAGG 
ACCAATTCTC CTAGCTCTGC TAAAAAACCT AAAGCACTTC AACACGCCGA AACTTCCTCG 
GAAAGTAACA AGCCACATAC TAAATCCAAG AGAAGACACT TAGACCAAGA ACAGCAGAAG 
TCTACACAAT TGCCATCAAC AAGCAAGGCT CACACCAGAA AGGGTGGAGC TGCTGGTAGC 
TCCCGAAGTC AGAAAAGGAA AAGGACAGAG AGTACGTCTT GTATAAAGAG TCGTTCAGCA 
GTTGAATCAA CTGGAGCTGA AGAGAAGTCA GCAAAATCCT CCAAGCTGGC TTCAAAATCG 
GTGACCTCAG CCAAAGCTGG GTGTAGCACC ATCACTGATT CTTCTTCTTC AGCTTCTACA 
TCCTCTTCCT CTTCTGCTGT TGCCTCTGCT TCTTCCACTG TTCCTCAGGG TGCCAGAGTG 
AAACAGGGTA AGGACCAGAA TAAGGCTAGA CGTTCACGTT CTGCGTCCAG CCCTAGTCCA 
AGAAGGAGCA GCAGGGACAA AGAACAGAGC AAAACAGGTG GCTCTTCAAA GTTTGACTGG 
GCTGCTCGAT TCAGTCCAAA AGTTAGTCTC CCTAAAACAA AACTGTCTCT ACCAGGCTCT 
TCTAAGTCAG AGACCTCAAA ACCTGGACCT TCAGGACTAC AGGCTAAGCT AGCAAGCCTA 
AGAAAATCCA CAAAGAAGCG CAGTGAATCA CCACCTGCTG AGCTCCCCAG TTTACGGCGG 
AGCACACGGC AAAAGACCAC GGGCTCCTGT GCGAGTACCA GTCGGCGGGG CTCTGGCCTG 
GGCAAAAGAG GAGCAGCTGA AGCTCGCCGA CAGGAGAAGA TGGCTGATCC TGACAACAAC 
CAGGATGGAG TTAACTCTTC AGCTGCACGT ACAGATGAGA CTCCCCAGGG AGCTGCAGCT 
TCTAGCTCTG TTGCTGGGGC TGTAGGTATG ACAACATCTG GAGAAAGTGA GTCAGATGAC 
TCTGAGATGG GAAGACTACA AGCTCTGTTA GAGGCTAGGG GTCTTCCTCC TCACCTGTTT 
GGCCCTCTTG GTCCTCGGAT GTCGCAGCTC TTCCACAGGA CAATTGGAAG TGGAGCTAGT 
TCTAAAGCCC AGCAGCTTTT ACAAGGTCTC CAAGCCACTG ATGAAAGTCA GCAACTACAG 
GCAGTTATTG AGATGTGCCA GCTGTTGGTC ATGGGAAATG AAGAAACACT GGGAGGATTT 
CCAGTCAAGA GTGTTGTGCC AGCTTTGATA ACATTGCTGC AGATGGAGCA TAACTTTGAC 
ATCATGAACC ATGCTTGTCG AGCCTTAACA TACATGATGG AAGCACTTCC CAGATCATCT 
GCTGTAGTGG TAGATGCAAT TCCTGTCTTC TTGGAAAAGC TGCAAGTTAT TCAGTGCATT 
GATGTGGCAG AGCAGGCACT GACAGCCCTA GAGATGCTAT CACGCAGGCA TAGTAAAGCC 
ATTCTGCAGG CAGGTGGGTT GGCAGACTGC TTGCTGTATC TGGAATTCTT CAGTATAAAT 
GCGCAGAGGA ATGCACTAGC TATTGCTGCC AATTGCTGCC AGAGTATAAC ACCTGATGAG 
TTTCACTTTG TGGCAGACTC TTTGCCACTG CTTACACAAA GGTTAACCCA TCAGGACAAG 
AAGTCTGTTG AAAGCACTTG TCTCTGTTTT GCACGGCTGG TAGACAATTT CCAGCATGAG 
GAGAACTTGC TGCAGCAGGT TGCTTCCAAG GACTTGTTAA CAAACATCCA GCAACTCTTG 
GTAGTGACAC CTCCTATCCT GAGCTCAGGA ATGTTCATCA TGGTGGTGCG CATGTTTTCC 
TTAATGTGTT CCAATTGTCC AACGCTTGCA GTCCAACTTA TGAAGCAAAA CATTGCAGAA 
ACACTTCACT TTCTCCTTTG TGGAGCCTCA AATGGGAGTT GTCAAGAACA AATTGACCTT 
GTTCCACGAA GTCCTCAAGA ACTCTATGAG CTTACTTCTC TTATCTGTGA ACTGATGCCT 
TGTCTGCCAA AAGAGGGGAT ATTTGCTGTT GATACTATGC TAAAGAAAGG AAATGCACAA 
AATACAGATG GTGCGATATG GCAATGGCGA GATGACAGGG GTCTCTGGCA TCCCTATAAC 
AGGATTGATA GTCGAATAAT AGAGGCAGCT CATCAGGTTG GTGAGGATGA GATAAGCCTG 
TCGACACTTG GACGTGTCTA TACTATTGAT TTTAATTCTA TGCAGCAAAT AAATGAGGAT 
ACTGGAACAG CACGTGCCAT TCAGCGGAAA CCAAACCCTT TAGCCAATAC AAACACTAGT 
GGACATTCAG AATTGAAGAA GGATGATGCT CGAGCACAGC TAATGAAAGA AGACCCAGAA 
TTGGCAAAAT CCTTTATCAA AACACTGTTT GGTGTTCTTT ATGAAGTGTA TAGTTCTTCA 
GCTGGACCTG CTGTTAGACA CAAGTGCCTT AGAGCAATAC TTAGAATAAT TTATTTTGCT 
GATGCTGAAC TCCTGAAGGA TGTGCTGAAA AACCATGCTG TGTCAAGTCA CATTGCCTCC 
ATGCTGTCAA GTCAAGACCT TAAGATAGTA GTTGGAGCCC TGCAGATGGC AGAAATCTTA 
ATGCAGAAGC TACCTGACAT TTTTAGTGTT TACTTCAGAA GAGAAGGAGT GATGCATCAA 
GTGAAAAACT TAGCGGAGTC TGAGGCTTTG CTAACGAGCC CACCAAAAGT ATGCACTAAT 
GGATCAGGAA CACTGGGTAC CACTACAACT ATAAGTACTG GAACAGCCAC TGCTGCCACT 
AATGCAGCTG CAGATTTGGG CTCTCCCAGC TTACAACACA GCCGGGAGGA TTCTTTAGAT 
CTGAGCCCAC AGGGACGACT GAGTGATGTT CTGAAGAGAA AAAGACTGCC AAAACGAGGG 
CCAAGGAGAC CAAAATATTC TCCCCCAAGA GATGATGACA AAGTAGACAA TCAAGCAAAA 
AGCCCCACGA CTACTCAGTC TCCTAAATCT TCTTTCTTAG CAAGTTTAAA TCCCAAAACA 
TGGGGAAGAT TAAGTACACA GTCTAACAGT AATAACATTG AACCAGCAAG AACAGCAGGT 
GTAAGTGGTC TTGCAAGAGC TGCTTCTAAG GATACAATTT CCAATAACAG AGAAAAAATT 
AAGGGTTGGA TTAAGGAGCA AGCCCATAAA TTTGTAGACC GTTATTTTAG TTCGGAAAAC 
ATGGATGGAA GCAATCCTGC ACTAAATGTA TTACAGAGAC TTTGCACTGC AACTGAACAA 
CTTAACCTCC AGGTGGATGG TGGAACAGAG TGCCTTGTAG AAATCCGTAG CATTGTCTCG 
GAGTCTGACG TCTCCTCATT TGAAATCCAG CATAGTGGAT TTGTGAAACA ACTGCTGCTT 
TATTTGACAT CTAAAAGTGA GAAAGATGCT GTAAGCAGGG ATATCAGATT GAAAAGATTT 
CTTCATGTAT TTTTCTCCTC TCCACTTCCT GGAGAAGAAC CCCTTGGAAG ATTAGAGCCA 
TTAGAAAATG CACCTTTGTT GGCATTAGTC CATAAAATGA ACAATTGCCT CAGTCAGATG 
GAGCAGTTTC CAGTGAAAGT GCATGACTTC CCCAGTGGAA ATGGAACAGG GAGCAGAGGA 
TCCCAAGCTT TAAAATTCTT CAATACGCAT CAGTTAAAAT GCCAGCTGCA AAGACATCCA 
GACTGTGCTA ATGTGAAACA GTGGAAGGGT GGACCTGTGA AGATTGATCC TCTGGCTTTG 
GTTCAAGCCA TTGAAAGATA TCTTGTTGTT AGAGGTTATG GAAGAGTTAG AGAAGATGAT 
GAGGATAGTG ATGATGATGG GTCAGATGAA GAAATAGATG AATCCTTGGC TGCTCAATTC 
TTAAATTCAG GGAATGTGAG ACATAGATTG CAGTTTTACA TTGGGGATCA CTTGCTACCA 
TATAATATGA CTGTATATCA AGCAGTTAAG CTGTACAGTT TGCAAGCTGA GGAGGAGAGG 
GAATCGACTG ATGATGAAAG CAATCCATTA GGAAGAGCTG GGATTTGGAC AAAAACACAT 
ACCATTTGGT ACAAACCTGT ACGGGAGGAT GAAGATGGCA ACAAAGACTG TGTTGGTGGT 
AAAAGAGGAA GAGCTCAAAC TGCTCCCACA AAAACCTCAC CTAGGAATTC TAAAAAGCAT 
GATGAGTTGT GGCACGATGG TGTATGCCCT TCAGTATTGA ATCCTTTAGA AGTTTACCTC 
ATATCTACTC CTCCTGAAAA CATAACCTTT GAAGATCCTT CATTAGATGT TATTCTTCTT 
TTGAGAGTTT TACATGCTAT CAGTCGATAC TGGTATTATT TGTATGATAA TGCAATATGC 
AAGGAGATTA TTCCAACTTC GGAATTTATC AATAGTAAAC TGACAGCAAA AGCAAACAGA 
CAGCTTCAAG ATCCACTGGT AATTATGACA GGAAACATAC CGACTTGGCT GACAGAGCTT 
GGAAAAACAT GCCCATTTTT CTTTCCATTT GATACCCGGC AAATGCTGTT TTATGTAACT 
GCTTTTGATC GTGATCGAGC CATGCAAAGA CTACTGGATA CTAATCCAGA AATCAATCAA 
TCAGATTCTC AGGATAGCAG AGTGGCACCA CGACTGGACA GGAAAAAACG CACTGTGAAC 
AGAGATGAGC TGTTGAAACA GGCAGAATCT GTGATGCAGG ATCTAGGCAG TTCAAGAGCC 
ATGTTGGAAA TCCAGTATGA GAATGAAGTT GGCACAGGCC TGGGCCCTAC ACTAGAGTTC 
TATGCACTTG TATCTCAGGA ACTACAGAGA GCAGACTTGG GCCTTTGGAG GGGAGAAGAA 
GTAACTTTAG CCAATCCAAA AGGAAGCCAA GAAGGTACCA AGTATATTCA TAATCTCCAA 
GGCCTTTTTG CACTTCCTTT TGGTAGAACA GCCAAGCCTG CTCACATTGC AAAAGTTAAA 
ATGAAGTTCC GCTTTCTGGG AAAACTAATG GCTAAGGCAA TCATGGATTT TAGACTGGTG 
GATCTTCCTC TTGGACTTCC TTTTTATAAA TGGATGTTAC GCCAGGAAAC TTCTTTGACG 
TCACATGACT TGTTCAGTAT TGATCCAGTA GTAGCCAAAT CAATATATCA CCTTGAAGAT 
ATTGTAAGAC AAAAGAAAAG ACTTGAGCAG GACAAAACAC AGACCAAAGA AAGTCTACAG 
TATGCATTGG AAACCCTGAC TATGAATGGC TGCTCAGTGG AGGATCTAGG GCTGGATTTC 
ACACTTCCTG GATTTCCTAA CATCGAGCTG AAAAAAGGGG GAAAAGATAC ACCAGTCACC 
ATCCACAACT TGGAGGAGTA CCTCAGATTG GTTATATTCT GGGCACTTAA CGAAGGTGTT 
GCCAGACAGT TTGATTCTTT CAGAGATGGA TTTGAATCAG TCTTCCCCCT CAGTCATCTT 
CAATACTTTT ATCCTGAGGA GTTGGAGCAA CTCTTATGTG GCAGCAAAAC AGACACATGG 
GATGCCAAGA CCTTAATGGA ATGTTGCAGG CCAGATCACG GTTATACACA TGACAGTCGA 
GCAGTGAAGT ATCTCTTTGA AATTCTCAGT AGTTTTGATA GTGAGCAGCA GAGACTGTTT 
CTTCAGTTTG TGACCGGTAG TCCCAGGCTG CCAGTAGGAG GCTTTCGAAG TTTGAATCCT 
CCATTGACAA TTGTACGGAA GACATTTGAG TCTACAGAGA ATCCAGATGA CTTCTTGCCC 
TCAGTAATGA CTTGTGTGAA CTATCTCAAA TTGCCGGACT ATTCAACTAT TGAGATAATG 
CGTGAAAAAC TCTTGATAGC TGCAAGAGAA GGGCAGCAGT CATTCCATCT TTCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TRIP12 ( XM_015276969.1 ) cDNA ORF clone, Gallus gallus(chicken) -> XP_015132455.1 Gallus gallus thyroid hormone receptor interactor 12 (TRIP12), transcript variant X8, mRNA.

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CloneID	OGa39716
Clone ID Related Accession (Same CDS sequence)	XM_015276969.1 , XM_015276969.2
Accession Version	XM_015276969.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6102bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-03
Organism	Gallus gallus(chicken)
Product	E3 ubiquitin-protein ligase TRIP12 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_001471743.3) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Gallus gallus Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCCAACC GGCCTAATAA CAATCCAGGG GGGTCACTGC GACGTTCACA GAGGAACACT 
GCTGGGGCCC AGCCACAAGA CGACGCAGCA GGAGGAAGGT CACATTTAGG GCAGGCAAAA 
CATAAGGCAC ATAGCCCTCC TGAGAGTAGA AAATCTATTT CAAAGACACC CAAAGTGCAG 
TCTAATACTA CTTCTGAGCA GTCCAAGGGA CACTTTTCTA AAAGAGGCTG TAGTTCATCT 
GCTGTTTTAA TACCACAAGA AGATTCAGAA AGAGTCAGTA CTTCAGAAAA GCAAAAAACG 
GGGCAAGTGC CTAAGAAAGA CAATTCTCGA GGAGTTAAAC GCAGTGCTAG TCCAGATTAC 
AGCAGGACCA ATTCTCCTAG CTCTGCTAAA AAACCTAAAG CACTTCAACA CGCCGAAACT 
TCCTCGGAAA GTAACAAGCC ACATACTAAA TCCAAGAGAA GACACTTAGA CCAAGAACAG 
CAGAAGTCTA CACAATTGCC ATCAACAAGC AAGGCTCACA CCAGAAAGGG TGGAGCTGCT 
GGTAGCTCCC GAAGTCAGAA AAGGAAAAGG ACAGAGAGTA CGTCTTGTAT AAAGAGTCGT 
TCAGCAGTTG AATCAACTGG AGCTGAAGAG AAGTCAGCAA AATCCTCCAA GCTGGCTTCA 
AAATCGGTGA CCTCAGCCAA AGCTGGGTGT AGCACCATCA CTGATTCTTC TTCTTCAGCT 
TCTACATCCT CTTCCTCTTC TGCTGTTGCC TCTGCTTCTT CCACTGTTCC TCAGGGTGCC 
AGAGTGAAAC AGGGTAAGGA CCAGAATAAG GCTAGACGTT CACGTTCTGC GTCCAGCCCT 
AGTCCAAGAA GGAGCAGCAG GGACAAAGAA CAGAGCAAAA CAGGTGGCTC TTCAAAGTTT 
GACTGGGCTG CTCGATTCAG TCCAAAAGTT AGTCTCCCTA AAACAAAACT GTCTCTACCA 
GGCTCTTCTA AGTCAGAGAC CTCAAAACCT GGACCTTCAG GACTACAGGC TAAGCTAGCA 
AGCCTAAGAA AATCCACAAA GAAGCGCAGT GAATCACCAC CTGCTGAGCT CCCCAGTTTA 
CGGCGGAGCA CACGGCAAAA GACCACGGGC TCCTGTGCGA GTACCAGTCG GCGGGGCTCT 
GGCCTGGGCA AAAGAGGAGC AGCTGAAGCT CGCCGACAGG AGAAGATGGC TGATCCTGAC 
AACAACCAGG ATGGAGTTAA CTCTTCAGCT GCACGTACAG ATGAGACTCC CCAGGGAGCT 
GCAGCTTCTA GCTCTGTTGC TGGGGCTGTA GGTATGACAA CATCTGGAGA AAGTGAGTCA 
GATGACTCTG AGATGGGAAG ACTACAAGGT TCTAAAGCCC AGCAGCTTTT ACAAGGTCTC 
CAAGCCACTG ATGAAAGTCA GCAACTACAG GCAGTTATTG AGATGTGCCA GCTGTTGGTC 
ATGGGAAATG AAGAAACACT GGGAGGATTT CCAGTCAAGA GTGTTGTGCC AGCTTTGATA 
ACATTGCTGC AGATGGAGCA TAACTTTGAC ATCATGAACC ATGCTTGTCG AGCCTTAACA 
TACATGATGG AAGCACTTCC CAGATCATCT GCTGTAGTGG TAGATGCAAT TCCTGTCTTC 
TTGGAAAAGC TGCAAGTTAT TCAGTGCATT GATGTGGCAG AGCAGGCACT GACAGCCCTA 
GAGATGCTAT CACGCAGGCA TAGTAAAGCC ATTCTGCAGG CAGGTGGGTT GGCAGACTGC 
TTGCTGTATC TGGAATTCTT CAGTATAAAT GCGCAGAGGA ATGCACTAGC TATTGCTGCC 
AATTGCTGCC AGAGTATAAC ACCTGATGAG TTTCACTTTG TGGCAGACTC TTTGCCACTG 
CTTACACAAA GGTTAACCCA TCAGGACAAG AAGTCTGTTG AAAGCACTTG TCTCTGTTTT 
GCACGGCTGG TAGACAATTT CCAGCATGAG GAGAACTTGC TGCAGCAGGT TGCTTCCAAG 
GACTTGTTAA CAAACATCCA GCAACTCTTG GTAGTGACAC CTCCTATCCT GAGCTCAGGA 
ATGTTCATCA TGGTGGTGCG CATGTTTTCC TTAATGTGTT CCAATTGTCC AACGCTTGCA 
GTCCAACTTA TGAAGCAAAA CATTGCAGAA ACACTTCACT TTCTCCTTTG TGGAGCCTCA 
AATGGGAGTT GTCAAGAACA AATTGACCTT GTTCCACGAA GTCCTCAAGA ACTCTATGAG 
CTTACTTCTC TTATCTGTGA ACTGATGCCT TGTCTGCCAA AAGAGGGGAT ATTTGCTGTT 
GATACTATGC TAAAGAAAGG AAATGCACAA AATACAGATG GTGCGATATG GCAATGGCGA 
GATGACAGGG GTCTCTGGCA TCCCTATAAC AGGATTGATA GTCGAATAAT AGAGGCAGCT 
CATCAGGTTG GTGAGGATGA GATAAGCCTG TCGACACTTG GACGTGTCTA TACTATTGAT 
TTTAATTCTA TGCAGCAAAT AAATGAGGAT ACTGGAACAG CACGTGCCAT TCAGCGGAAA 
CCAAACCCTT TAGCCAATAC AAACACTAGT GGACATTCAG AATTGAAGAA GGATGATGCT 
CGAGCACAGC TAATGAAAGA AGACCCAGAA TTGGCAAAAT CCTTTATCAA AACACTGTTT 
GGTGTTCTTT ATGAAGTGTA TAGTTCTTCA GCTGGACCTG CTGTTAGACA CAAGTGCCTT 
AGAGCAATAC TTAGAATAAT TTATTTTGCT GATGCTGAAC TCCTGAAGGA TGTGCTGAAA 
AACCATGCTG TGTCAAGTCA CATTGCCTCC ATGCTGTCAA GTCAAGACCT TAAGATAGTA 
GTTGGAGCCC TGCAGATGGC AGAAATCTTA ATGCAGAAGC TACCTGACAT TTTTAGTGTT 
TACTTCAGAA GAGAAGGAGT GATGCATCAA GTGAAAAACT TAGCGGAGTC TGAGGCTTTG 
CTAACGAGCC CACCAAAAGT ATGCACTAAT GGATCAGGAA CACTGGGTAC CACTACAACT 
ATAAGTACTG GAACAGCCAC TGCTGCCACT AATGCAGCTG CAGATTTGGG CTCTCCCAGC 
TTACAACACA GCCGGGAGGA TTCTTTAGAT CTGAGCCCAC AGGGACGACT GAGTGATGTT 
CTGAAGAGAA AAAGACTGCC AAAACGAGGG CCAAGGAGAC CAAAATATTC TCCCCCAAGA 
GATGATGACA AAGTAGACAA TCAAGCAAAA AGCCCCACGA CTACTCAGTC TCCTAAATCT 
TCTTTCTTAG CAAGTTTAAA TCCCAAAACA TGGGGAAGAT TAAGTACACA GTCTAACAGT 
AATAACATTG AACCAGCAAG AACAGCAGGT GTAAGTGGTC TTGCAAGAGC TGCTTCTAAG 
GATACAATTT CCAATAACAG AGAAAAAATT AAGGGTTGGA TTAAGGAGCA AGCCCATAAA 
TTTGTAGACC GTTATTTTAG TTCGGAAAAC ATGGATGGAA GCAATCCTGC ACTAAATGTA 
TTACAGAGAC TTTGCACTGC AACTGAACAA CTTAACCTCC AGGTGGATGG TGGAACAGAG 
TGCCTTGTAG AAATCCGTAG CATTGTCTCG GAGTCTGACG TCTCCTCATT TGAAATCCAG 
CATAGTGGAT TTGTGAAACA ACTGCTGCTT TATTTGACAT CTAAAAGTGA GAAAGATGCT 
GTAAGCAGGG ATATCAGATT GAAAAGATTT CTTCATGTAT TTTTCTCCTC TCCACTTCCT 
GGAGAAGAAC CCCTTGGAAG ATTAGAGCCA TTAGAAAATG CACCTTTGTT GGCATTAGTC 
CATAAAATGA ACAATTGCCT CAGTCAGATG GAGCAGTTTC CAGTGAAAGT GCATGACTTC 
CCCAGTGGAA ATGGAACAGG GAGCAGTTTT TCTCTTAACA GAGGATCCCA AGCTTTAAAA 
TTCTTCAATA CGCATCAGTT AAAATGCCAG CTGCAAAGAC ATCCAGACTG TGCTAATGTG 
AAACAGTGGA AGGGTGGACC TGTGAAGATT GATCCTCTGG CTTTGGTTCA AGCCATTGAA 
AGATATCTTG TTGTTAGAGG TTATGGAAGA GTTAGAGAAG ATGATGAGGA TAGTGATGAT 
GATGGGTCAG ATGAAGAAAT AGATGAATCC TTGGCTGCTC AATTCTTAAA TTCAGGGAAT 
GTGAGACATA GATTGCAGTT TTACATTGGG GATCACTTGC TACCATATAA TATGACTGTA 
TATCAAGCAG TTAAGCTGTA CAGTTTGCAA GCTGAGGAGG AGAGGGAATC GACTGATGAT 
GAAAGCAATC CATTAGGAAG AGCTGGGATT TGGACAAAAA CACATACCAT TTGGTACAAA 
CCTGTACGGG AGGATGAAGA TGGCAACAAA GACTGTGTTG GTGGTAAAAG AGGAAGAGCT 
CAAACTGCTC CCACAAAAAC CTCACCTAGG AATTCTAAAA AGCATGATGA GTTGTGGCAC 
GATGGTGTAT GCCCTTCAGT ATTGAATCCT TTAGAAGTTT ACCTCATATC TACTCCTCCT 
GAAAACATAA CCTTTGAAGA TCCTTCATTA GATGTTATTC TTCTTTTGAG AGTTTTACAT 
GCTATCAGTC GATACTGGTA TTATTTGTAT GATAATGCAA TATGCAAGGA GATTATTCCA 
ACTTCGGAAT TTATCAATAG TAAACTGACA GCAAAAGCAA ACAGACAGCT TCAAGATCCA 
CTGGTAATTA TGACAGGAAA CATACCGACT TGGCTGACAG AGCTTGGAAA AACATGCCCA 
TTTTTCTTTC CATTTGATAC CCGGCAAATG CTGTTTTATG TAACTGCTTT TGATCGTGAT 
CGAGCCATGC AAAGACTACT GGATACTAAT CCAGAAATCA ATCAATCAGA TTCTCAGGAT 
AGCAGAGTGG CACCACGACT GGACAGGAAA AAACGCACTG TGAACAGAGA TGAGCTGTTG 
AAACAGGCAG AATCTGTGAT GCAGGATCTA GGCAGTTCAA GAGCCATGTT GGAAATCCAG 
TATGAGAATG AAGTTGGCAC AGGCCTGGGC CCTACACTAG AGTTCTATGC ACTTGTATCT 
CAGGAACTAC AGAGAGCAGA CTTGGGCCTT TGGAGGGGAG AAGAAGTAAC TTTAGCCAAT 
CCAAAAGGAA GCCAAGAAGG TACCAAGTAT ATTCATAATC TCCAAGGCCT TTTTGCACTT 
CCTTTTGGTA GAACAGCCAA GCCTGCTCAC ATTGCAAAAG TTAAAATGAA GTTCCGCTTT 
CTGGGAAAAC TAATGGCTAA GGCAATCATG GATTTTAGAC TGGTGGATCT TCCTCTTGGA 
CTTCCTTTTT ATAAATGGAT GTTACGCCAG GAAACTTCTT TGACGTCACA TGACTTGTTC 
AGTATTGATC CAGTAGTAGC CAAATCAATA TATCACCTTG AAGATATTGT AAGACAAAAG 
AAAAGACTTG AGCAGGACAA AACACAGACC AAAGAAAGTC TACAGTATGC ATTGGAAACC 
CTGACTATGA ATGGCTGCTC AGTGGAGGAT CTAGGGCTGG ATTTCACACT TCCTGGATTT 
CCTAACATCG AGCTGAAAAA AGGGGGAAAA GATACACCAG TCACCATCCA CAACTTGGAG 
GAGTACCTCA GATTGGTTAT ATTCTGGGCA CTTAACGAAG GTGTTGCCAG ACAGTTTGAT 
TCTTTCAGAG ATGGATTTGA ATCAGTCTTC CCCCTCAGTC ATCTTCAATA CTTTTATCCT 
GAGGAGTTGG AGCAACTCTT ATGTGGCAGC AAAACAGACA CATGGGATGC CAAGACCTTA 
ATGGAATGTT GCAGGCCAGA TCACGGTTAT ACACATGACA GTCGAGCAGT GAAGTATCTC 
TTTGAAATTC TCAGTAGTTT TGATAGTGAG CAGCAGAGAC TGTTTCTTCA GTTTGTGACC 
GGTAGTCCCA GGCTGCCAGT AGGAGGCTTT CGAAGTTTGA ATCCTCCATT GACAATTGTA 
CGGAAGACAT TTGAGTCTAC AGAGAATCCA GATGACTTCT TGCCCTCAGT AATGACTTGT 
GTGAACTATC TCAAATTGCC GGACTATTCA ACTATTGAGA TAATGCGTGA AAAACTCTTG 
ATAGCTGCAA GAGAAGGGCA GCAGTCATTC CATCTTTCCT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015132455.1
CDS	150..6251
Translation	MSNRPNNNPGGSLRRSQRNTAGAQPQDDAAGGRSHLGQAKHKAHSPPESRKSISKTPKVQSNTTSEQSKGHFSKRGCSSSAVLIPQEDSERVSTSEKQKTGQVPKKDNSRGVKRSASPDYSRTNSPSSAKKPKALQHAETSSESNKPHTKSKRRHLDQEQQKSTQLPSTSKAHTRKGGAAGSSRSQKRKRTESTSCIKSRSAVESTGAEEKSAKSSKLASKSVTSAKAGCSTITDSSSSASTSSSSSAVASASSTVPQGARVKQGKDQNKARRSRSASSPSPRRSSRDKEQSKTGGSSKFDWAARFSPKVSLPKTKLSLPGSSKSETSKPGPSGLQAKLASLRKSTKKRSESPPAELPSLRRSTRQKTTGSCASTSRRGSGLGKRGAAEARRQEKMADPDNNQDGVNSSAARTDETPQGAAASSSVAGAVGMTTSGESESDDSEMGRLQGSKAQQLLQGLQATDESQQLQAVIEMCQLLVMGNEETLGGFPVKSVVPALITLLQMEHNFDIMNHACRALTYMMEALPRSSAVVVDAIPVFLEKLQVIQCIDVAEQALTALEMLSRRHSKAILQAGGLADCLLYLEFFSINAQRNALAIAANCCQSITPDEFHFVADSLPLLTQRLTHQDKKSVESTCLCFARLVDNFQHEENLLQQVASKDLLTNIQQLLVVTPPILSSGMFIMVVRMFSLMCSNCPTLAVQLMKQNIAETLHFLLCGASNGSCQEQIDLVPRSPQELYELTSLICELMPCLPKEGIFAVDTMLKKGNAQNTDGAIWQWRDDRGLWHPYNRIDSRIIEAAHQVGEDEISLSTLGRVYTIDFNSMQQINEDTGTARAIQRKPNPLANTNTSGHSELKKDDARAQLMKEDPELAKSFIKTLFGVLYEVYSSSAGPAVRHKCLRAILRIIYFADAELLKDVLKNHAVSSHIASMLSSQDLKIVVGALQMAEILMQKLPDIFSVYFRREGVMHQVKNLAESEALLTSPPKVCTNGSGTLGTTTTISTGTATAATNAAADLGSPSLQHSREDSLDLSPQGRLSDVLKRKRLPKRGPRRPKYSPPRDDDKVDNQAKSPTTTQSPKSSFLASLNPKTWGRLSTQSNSNNIEPARTAGVSGLARAASKDTISNNREKIKGWIKEQAHKFVDRYFSSENMDGSNPALNVLQRLCTATEQLNLQVDGGTECLVEIRSIVSESDVSSFEIQHSGFVKQLLLYLTSKSEKDAVSRDIRLKRFLHVFFSSPLPGEEPLGRLEPLENAPLLALVHKMNNCLSQMEQFPVKVHDFPSGNGTGSSFSLNRGSQALKFFNTHQLKCQLQRHPDCANVKQWKGGPVKIDPLALVQAIERYLVVRGYGRVREDDEDSDDDGSDEEIDESLAAQFLNSGNVRHRLQFYIGDHLLPYNMTVYQAVKLYSLQAEEERESTDDESNPLGRAGIWTKTHTIWYKPVREDEDGNKDCVGGKRGRAQTAPTKTSPRNSKKHDELWHDGVCPSVLNPLEVYLISTPPENITFEDPSLDVILLLRVLHAISRYWYYLYDNAICKEIIPTSEFINSKLTAKANRQLQDPLVIMTGNIPTWLTELGKTCPFFFPFDTRQMLFYVTAFDRDRAMQRLLDTNPEINQSDSQDSRVAPRLDRKKRTVNRDELLKQAESVMQDLGSSRAMLEIQYENEVGTGLGPTLEFYALVSQELQRADLGLWRGEEVTLANPKGSQEGTKYIHNLQGLFALPFGRTAKPAHIAKVKMKFRFLGKLMAKAIMDFRLVDLPLGLPFYKWMLRQETSLTSHDLFSIDPVVAKSIYHLEDIVRQKKRLEQDKTQTKESLQYALETLTMNGCSVEDLGLDFTLPGFPNIELKKGGKDTPVTIHNLEEYLRLVIFWALNEGVARQFDSFRDGFESVFPLSHLQYFYPEELEQLLCGSKTDTWDAKTLMECCRPDHGYTHDSRAVKYLFEILSSFDSEQQRLFLQFVTGSPRLPVGGFRSLNPPLTIVRKTFESTENPDDFLPSVMTCVNYLKLPDYSTIEIMREKLLIAAREGQQSFHLS

Target ORF information:

RefSeq Version	XM_015276969.1
Organism	Gallus gallus(chicken)
Definition	Gallus gallus thyroid hormone receptor interactor 12 (TRIP12), transcript variant X8, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015276969.1

ORF Insert Sequence:

ATGTCCAACC GGCCTAATAA CAATCCAGGG GGGTCACTGC GACGTTCACA GAGGAACACT 
GCTGGGGCCC AGCCACAAGA CGACGCAGCA GGAGGAAGGT CACATTTAGG GCAGGCAAAA 
CATAAGGCAC ATAGCCCTCC TGAGAGTAGA AAATCTATTT CAAAGACACC CAAAGTGCAG 
TCTAATACTA CTTCTGAGCA GTCCAAGGGA CACTTTTCTA AAAGAGGCTG TAGTTCATCT 
GCTGTTTTAA TACCACAAGA AGATTCAGAA AGAGTCAGTA CTTCAGAAAA GCAAAAAACG 
GGGCAAGTGC CTAAGAAAGA CAATTCTCGA GGAGTTAAAC GCAGTGCTAG TCCAGATTAC 
AGCAGGACCA ATTCTCCTAG CTCTGCTAAA AAACCTAAAG CACTTCAACA CGCCGAAACT 
TCCTCGGAAA GTAACAAGCC ACATACTAAA TCCAAGAGAA GACACTTAGA CCAAGAACAG 
CAGAAGTCTA CACAATTGCC ATCAACAAGC AAGGCTCACA CCAGAAAGGG TGGAGCTGCT 
GGTAGCTCCC GAAGTCAGAA AAGGAAAAGG ACAGAGAGTA CGTCTTGTAT AAAGAGTCGT 
TCAGCAGTTG AATCAACTGG AGCTGAAGAG AAGTCAGCAA AATCCTCCAA GCTGGCTTCA 
AAATCGGTGA CCTCAGCCAA AGCTGGGTGT AGCACCATCA CTGATTCTTC TTCTTCAGCT 
TCTACATCCT CTTCCTCTTC TGCTGTTGCC TCTGCTTCTT CCACTGTTCC TCAGGGTGCC 
AGAGTGAAAC AGGGTAAGGA CCAGAATAAG GCTAGACGTT CACGTTCTGC GTCCAGCCCT 
AGTCCAAGAA GGAGCAGCAG GGACAAAGAA CAGAGCAAAA CAGGTGGCTC TTCAAAGTTT 
GACTGGGCTG CTCGATTCAG TCCAAAAGTT AGTCTCCCTA AAACAAAACT GTCTCTACCA 
GGCTCTTCTA AGTCAGAGAC CTCAAAACCT GGACCTTCAG GACTACAGGC TAAGCTAGCA 
AGCCTAAGAA AATCCACAAA GAAGCGCAGT GAATCACCAC CTGCTGAGCT CCCCAGTTTA 
CGGCGGAGCA CACGGCAAAA GACCACGGGC TCCTGTGCGA GTACCAGTCG GCGGGGCTCT 
GGCCTGGGCA AAAGAGGAGC AGCTGAAGCT CGCCGACAGG AGAAGATGGC TGATCCTGAC 
AACAACCAGG ATGGAGTTAA CTCTTCAGCT GCACGTACAG ATGAGACTCC CCAGGGAGCT 
GCAGCTTCTA GCTCTGTTGC TGGGGCTGTA GGTATGACAA CATCTGGAGA AAGTGAGTCA 
GATGACTCTG AGATGGGAAG ACTACAAGGT TCTAAAGCCC AGCAGCTTTT ACAAGGTCTC 
CAAGCCACTG ATGAAAGTCA GCAACTACAG GCAGTTATTG AGATGTGCCA GCTGTTGGTC 
ATGGGAAATG AAGAAACACT GGGAGGATTT CCAGTCAAGA GTGTTGTGCC AGCTTTGATA 
ACATTGCTGC AGATGGAGCA TAACTTTGAC ATCATGAACC ATGCTTGTCG AGCCTTAACA 
TACATGATGG AAGCACTTCC CAGATCATCT GCTGTAGTGG TAGATGCAAT TCCTGTCTTC 
TTGGAAAAGC TGCAAGTTAT TCAGTGCATT GATGTGGCAG AGCAGGCACT GACAGCCCTA 
GAGATGCTAT CACGCAGGCA TAGTAAAGCC ATTCTGCAGG CAGGTGGGTT GGCAGACTGC 
TTGCTGTATC TGGAATTCTT CAGTATAAAT GCGCAGAGGA ATGCACTAGC TATTGCTGCC 
AATTGCTGCC AGAGTATAAC ACCTGATGAG TTTCACTTTG TGGCAGACTC TTTGCCACTG 
CTTACACAAA GGTTAACCCA TCAGGACAAG AAGTCTGTTG AAAGCACTTG TCTCTGTTTT 
GCACGGCTGG TAGACAATTT CCAGCATGAG GAGAACTTGC TGCAGCAGGT TGCTTCCAAG 
GACTTGTTAA CAAACATCCA GCAACTCTTG GTAGTGACAC CTCCTATCCT GAGCTCAGGA 
ATGTTCATCA TGGTGGTGCG CATGTTTTCC TTAATGTGTT CCAATTGTCC AACGCTTGCA 
GTCCAACTTA TGAAGCAAAA CATTGCAGAA ACACTTCACT TTCTCCTTTG TGGAGCCTCA 
AATGGGAGTT GTCAAGAACA AATTGACCTT GTTCCACGAA GTCCTCAAGA ACTCTATGAG 
CTTACTTCTC TTATCTGTGA ACTGATGCCT TGTCTGCCAA AAGAGGGGAT ATTTGCTGTT 
GATACTATGC TAAAGAAAGG AAATGCACAA AATACAGATG GTGCGATATG GCAATGGCGA 
GATGACAGGG GTCTCTGGCA TCCCTATAAC AGGATTGATA GTCGAATAAT AGAGGCAGCT 
CATCAGGTTG GTGAGGATGA GATAAGCCTG TCGACACTTG GACGTGTCTA TACTATTGAT 
TTTAATTCTA TGCAGCAAAT AAATGAGGAT ACTGGAACAG CACGTGCCAT TCAGCGGAAA 
CCAAACCCTT TAGCCAATAC AAACACTAGT GGACATTCAG AATTGAAGAA GGATGATGCT 
CGAGCACAGC TAATGAAAGA AGACCCAGAA TTGGCAAAAT CCTTTATCAA AACACTGTTT 
GGTGTTCTTT ATGAAGTGTA TAGTTCTTCA GCTGGACCTG CTGTTAGACA CAAGTGCCTT 
AGAGCAATAC TTAGAATAAT TTATTTTGCT GATGCTGAAC TCCTGAAGGA TGTGCTGAAA 
AACCATGCTG TGTCAAGTCA CATTGCCTCC ATGCTGTCAA GTCAAGACCT TAAGATAGTA 
GTTGGAGCCC TGCAGATGGC AGAAATCTTA ATGCAGAAGC TACCTGACAT TTTTAGTGTT 
TACTTCAGAA GAGAAGGAGT GATGCATCAA GTGAAAAACT TAGCGGAGTC TGAGGCTTTG 
CTAACGAGCC CACCAAAAGT ATGCACTAAT GGATCAGGAA CACTGGGTAC CACTACAACT 
ATAAGTACTG GAACAGCCAC TGCTGCCACT AATGCAGCTG CAGATTTGGG CTCTCCCAGC 
TTACAACACA GCCGGGAGGA TTCTTTAGAT CTGAGCCCAC AGGGACGACT GAGTGATGTT 
CTGAAGAGAA AAAGACTGCC AAAACGAGGG CCAAGGAGAC CAAAATATTC TCCCCCAAGA 
GATGATGACA AAGTAGACAA TCAAGCAAAA AGCCCCACGA CTACTCAGTC TCCTAAATCT 
TCTTTCTTAG CAAGTTTAAA TCCCAAAACA TGGGGAAGAT TAAGTACACA GTCTAACAGT 
AATAACATTG AACCAGCAAG AACAGCAGGT GTAAGTGGTC TTGCAAGAGC TGCTTCTAAG 
GATACAATTT CCAATAACAG AGAAAAAATT AAGGGTTGGA TTAAGGAGCA AGCCCATAAA 
TTTGTAGACC GTTATTTTAG TTCGGAAAAC ATGGATGGAA GCAATCCTGC ACTAAATGTA 
TTACAGAGAC TTTGCACTGC AACTGAACAA CTTAACCTCC AGGTGGATGG TGGAACAGAG 
TGCCTTGTAG AAATCCGTAG CATTGTCTCG GAGTCTGACG TCTCCTCATT TGAAATCCAG 
CATAGTGGAT TTGTGAAACA ACTGCTGCTT TATTTGACAT CTAAAAGTGA GAAAGATGCT 
GTAAGCAGGG ATATCAGATT GAAAAGATTT CTTCATGTAT TTTTCTCCTC TCCACTTCCT 
GGAGAAGAAC CCCTTGGAAG ATTAGAGCCA TTAGAAAATG CACCTTTGTT GGCATTAGTC 
CATAAAATGA ACAATTGCCT CAGTCAGATG GAGCAGTTTC CAGTGAAAGT GCATGACTTC 
CCCAGTGGAA ATGGAACAGG GAGCAGTTTT TCTCTTAACA GAGGATCCCA AGCTTTAAAA 
TTCTTCAATA CGCATCAGTT AAAATGCCAG CTGCAAAGAC ATCCAGACTG TGCTAATGTG 
AAACAGTGGA AGGGTGGACC TGTGAAGATT GATCCTCTGG CTTTGGTTCA AGCCATTGAA 
AGATATCTTG TTGTTAGAGG TTATGGAAGA GTTAGAGAAG ATGATGAGGA TAGTGATGAT 
GATGGGTCAG ATGAAGAAAT AGATGAATCC TTGGCTGCTC AATTCTTAAA TTCAGGGAAT 
GTGAGACATA GATTGCAGTT TTACATTGGG GATCACTTGC TACCATATAA TATGACTGTA 
TATCAAGCAG TTAAGCTGTA CAGTTTGCAA GCTGAGGAGG AGAGGGAATC GACTGATGAT 
GAAAGCAATC CATTAGGAAG AGCTGGGATT TGGACAAAAA CACATACCAT TTGGTACAAA 
CCTGTACGGG AGGATGAAGA TGGCAACAAA GACTGTGTTG GTGGTAAAAG AGGAAGAGCT 
CAAACTGCTC CCACAAAAAC CTCACCTAGG AATTCTAAAA AGCATGATGA GTTGTGGCAC 
GATGGTGTAT GCCCTTCAGT ATTGAATCCT TTAGAAGTTT ACCTCATATC TACTCCTCCT 
GAAAACATAA CCTTTGAAGA TCCTTCATTA GATGTTATTC TTCTTTTGAG AGTTTTACAT 
GCTATCAGTC GATACTGGTA TTATTTGTAT GATAATGCAA TATGCAAGGA GATTATTCCA 
ACTTCGGAAT TTATCAATAG TAAACTGACA GCAAAAGCAA ACAGACAGCT TCAAGATCCA 
CTGGTAATTA TGACAGGAAA CATACCGACT TGGCTGACAG AGCTTGGAAA AACATGCCCA 
TTTTTCTTTC CATTTGATAC CCGGCAAATG CTGTTTTATG TAACTGCTTT TGATCGTGAT 
CGAGCCATGC AAAGACTACT GGATACTAAT CCAGAAATCA ATCAATCAGA TTCTCAGGAT 
AGCAGAGTGG CACCACGACT GGACAGGAAA AAACGCACTG TGAACAGAGA TGAGCTGTTG 
AAACAGGCAG AATCTGTGAT GCAGGATCTA GGCAGTTCAA GAGCCATGTT GGAAATCCAG 
TATGAGAATG AAGTTGGCAC AGGCCTGGGC CCTACACTAG AGTTCTATGC ACTTGTATCT 
CAGGAACTAC AGAGAGCAGA CTTGGGCCTT TGGAGGGGAG AAGAAGTAAC TTTAGCCAAT 
CCAAAAGGAA GCCAAGAAGG TACCAAGTAT ATTCATAATC TCCAAGGCCT TTTTGCACTT 
CCTTTTGGTA GAACAGCCAA GCCTGCTCAC ATTGCAAAAG TTAAAATGAA GTTCCGCTTT 
CTGGGAAAAC TAATGGCTAA GGCAATCATG GATTTTAGAC TGGTGGATCT TCCTCTTGGA 
CTTCCTTTTT ATAAATGGAT GTTACGCCAG GAAACTTCTT TGACGTCACA TGACTTGTTC 
AGTATTGATC CAGTAGTAGC CAAATCAATA TATCACCTTG AAGATATTGT AAGACAAAAG 
AAAAGACTTG AGCAGGACAA AACACAGACC AAAGAAAGTC TACAGTATGC ATTGGAAACC 
CTGACTATGA ATGGCTGCTC AGTGGAGGAT CTAGGGCTGG ATTTCACACT TCCTGGATTT 
CCTAACATCG AGCTGAAAAA AGGGGGAAAA GATACACCAG TCACCATCCA CAACTTGGAG 
GAGTACCTCA GATTGGTTAT ATTCTGGGCA CTTAACGAAG GTGTTGCCAG ACAGTTTGAT 
TCTTTCAGAG ATGGATTTGA ATCAGTCTTC CCCCTCAGTC ATCTTCAATA CTTTTATCCT 
GAGGAGTTGG AGCAACTCTT ATGTGGCAGC AAAACAGACA CATGGGATGC CAAGACCTTA 
ATGGAATGTT GCAGGCCAGA TCACGGTTAT ACACATGACA GTCGAGCAGT GAAGTATCTC 
TTTGAAATTC TCAGTAGTTT TGATAGTGAG CAGCAGAGAC TGTTTCTTCA GTTTGTGACC 
GGTAGTCCCA GGCTGCCAGT AGGAGGCTTT CGAAGTTTGA ATCCTCCATT GACAATTGTA 
CGGAAGACAT TTGAGTCTAC AGAGAATCCA GATGACTTCT TGCCCTCAGT AATGACTTGT 
GTGAACTATC TCAAATTGCC GGACTATTCA ACTATTGAGA TAATGCGTGA AAAACTCTTG 
ATAGCTGCAA GAGAAGGGCA GCAGTCATTC CATCTTTCCT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TRIP12 ( XM_015276969.2 ) cDNA ORF clone, Gallus gallus(chicken) -> XP_015132455.1 Gallus gallus thyroid hormone receptor interactor 12 (TRIP12), transcript variant X7, mRNA.

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CloneID	OGa39716
Clone ID Related Accession (Same CDS sequence)	XM_015276969.1 , XM_015276969.2
Accession Version	XM_015276969.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6102bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-16
Organism	Gallus gallus(chicken)
Product	E3 ubiquitin-protein ligase TRIP12 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006096.5) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On May 17, 2018 this sequence version replaced XM_015276969.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Gallus gallus Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCCAACC GGCCTAATAA CAATCCAGGG GGGTCACTGC GACGTTCACA GAGGAACACT 
GCTGGGGCCC AGCCACAAGA CGACGCAGCA GGAGGAAGGT CACATTTAGG GCAGGCAAAA 
CATAAGGCAC ATAGCCCTCC TGAGAGTAGA AAATCTATTT CAAAGACACC CAAAGTGCAG 
TCTAATACTA CTTCTGAGCA GTCCAAGGGA CACTTTTCTA AAAGAGGCTG TAGTTCATCT 
GCTGTTTTAA TACCACAAGA AGATTCAGAA AGAGTCAGTA CTTCAGAAAA GCAAAAAACG 
GGGCAAGTGC CTAAGAAAGA CAATTCTCGA GGAGTTAAAC GCAGTGCTAG TCCAGATTAC 
AGCAGGACCA ATTCTCCTAG CTCTGCTAAA AAACCTAAAG CACTTCAACA CGCCGAAACT 
TCCTCGGAAA GTAACAAGCC ACATACTAAA TCCAAGAGAA GACACTTAGA CCAAGAACAG 
CAGAAGTCTA CACAATTGCC ATCAACAAGC AAGGCTCACA CCAGAAAGGG TGGAGCTGCT 
GGTAGCTCCC GAAGTCAGAA AAGGAAAAGG ACAGAGAGTA CGTCTTGTAT AAAGAGTCGT 
TCAGCAGTTG AATCAACTGG AGCTGAAGAG AAGTCAGCAA AATCCTCCAA GCTGGCTTCA 
AAATCGGTGA CCTCAGCCAA AGCTGGGTGT AGCACCATCA CTGATTCTTC TTCTTCAGCT 
TCTACATCCT CTTCCTCTTC TGCTGTTGCC TCTGCTTCTT CCACTGTTCC TCAGGGTGCC 
AGAGTGAAAC AGGGTAAGGA CCAGAATAAG GCTAGACGTT CACGTTCTGC GTCCAGCCCT 
AGTCCAAGAA GGAGCAGCAG GGACAAAGAA CAGAGCAAAA CAGGTGGCTC TTCAAAGTTT 
GACTGGGCTG CTCGATTCAG TCCAAAAGTT AGTCTCCCTA AAACAAAACT GTCTCTACCA 
GGCTCTTCTA AGTCAGAGAC CTCAAAACCT GGACCTTCAG GACTACAGGC TAAGCTAGCA 
AGCCTAAGAA AATCCACAAA GAAGCGCAGT GAATCACCAC CTGCTGAGCT CCCCAGTTTA 
CGGCGGAGCA CACGGCAAAA GACCACGGGC TCCTGTGCGA GTACCAGTCG GCGGGGCTCT 
GGCCTGGGCA AAAGAGGAGC AGCTGAAGCT CGCCGACAGG AGAAGATGGC TGATCCTGAC 
AACAACCAGG ATGGAGTTAA CTCTTCAGCT GCACGTACAG ATGAGACTCC CCAGGGAGCT 
GCAGCTTCTA GCTCTGTTGC TGGGGCTGTA GGTATGACAA CATCTGGAGA AAGTGAGTCA 
GATGACTCTG AGATGGGAAG ACTACAAGGT TCTAAAGCCC AGCAGCTTTT ACAAGGTCTC 
CAAGCCACTG ATGAAAGTCA GCAACTACAG GCAGTTATTG AGATGTGCCA GCTGTTGGTC 
ATGGGAAATG AAGAAACACT GGGAGGATTT CCAGTCAAGA GTGTTGTGCC AGCTTTGATA 
ACATTGCTGC AGATGGAGCA TAACTTTGAC ATCATGAACC ATGCTTGTCG AGCCTTAACA 
TACATGATGG AAGCACTTCC CAGATCATCT GCTGTAGTGG TAGATGCAAT TCCTGTCTTC 
TTGGAAAAGC TGCAAGTTAT TCAGTGCATT GATGTGGCAG AGCAGGCACT GACAGCCCTA 
GAGATGCTAT CACGCAGGCA TAGTAAAGCC ATTCTGCAGG CAGGTGGGTT GGCAGACTGC 
TTGCTGTATC TGGAATTCTT CAGTATAAAT GCGCAGAGGA ATGCACTAGC TATTGCTGCC 
AATTGCTGCC AGAGTATAAC ACCTGATGAG TTTCACTTTG TGGCAGACTC TTTGCCACTG 
CTTACACAAA GGTTAACCCA TCAGGACAAG AAGTCTGTTG AAAGCACTTG TCTCTGTTTT 
GCACGGCTGG TAGACAATTT CCAGCATGAG GAGAACTTGC TGCAGCAGGT TGCTTCCAAG 
GACTTGTTAA CAAACATCCA GCAACTCTTG GTAGTGACAC CTCCTATCCT GAGCTCAGGA 
ATGTTCATCA TGGTGGTGCG CATGTTTTCC TTAATGTGTT CCAATTGTCC AACGCTTGCA 
GTCCAACTTA TGAAGCAAAA CATTGCAGAA ACACTTCACT TTCTCCTTTG TGGAGCCTCA 
AATGGGAGTT GTCAAGAACA AATTGACCTT GTTCCACGAA GTCCTCAAGA ACTCTATGAG 
CTTACTTCTC TTATCTGTGA ACTGATGCCT TGTCTGCCAA AAGAGGGGAT ATTTGCTGTT 
GATACTATGC TAAAGAAAGG AAATGCACAA AATACAGATG GTGCGATATG GCAATGGCGA 
GATGACAGGG GTCTCTGGCA TCCCTATAAC AGGATTGATA GTCGAATAAT AGAGGCAGCT 
CATCAGGTTG GTGAGGATGA GATAAGCCTG TCGACACTTG GACGTGTCTA TACTATTGAT 
TTTAATTCTA TGCAGCAAAT AAATGAGGAT ACTGGAACAG CACGTGCCAT TCAGCGGAAA 
CCAAACCCTT TAGCCAATAC AAACACTAGT GGACATTCAG AATTGAAGAA GGATGATGCT 
CGAGCACAGC TAATGAAAGA AGACCCAGAA TTGGCAAAAT CCTTTATCAA AACACTGTTT 
GGTGTTCTTT ATGAAGTGTA TAGTTCTTCA GCTGGACCTG CTGTTAGACA CAAGTGCCTT 
AGAGCAATAC TTAGAATAAT TTATTTTGCT GATGCTGAAC TCCTGAAGGA TGTGCTGAAA 
AACCATGCTG TGTCAAGTCA CATTGCCTCC ATGCTGTCAA GTCAAGACCT TAAGATAGTA 
GTTGGAGCCC TGCAGATGGC AGAAATCTTA ATGCAGAAGC TACCTGACAT TTTTAGTGTT 
TACTTCAGAA GAGAAGGAGT GATGCATCAA GTGAAAAACT TAGCGGAGTC TGAGGCTTTG 
CTAACGAGCC CACCAAAAGT ATGCACTAAT GGATCAGGAA CACTGGGTAC CACTACAACT 
ATAAGTACTG GAACAGCCAC TGCTGCCACT AATGCAGCTG CAGATTTGGG CTCTCCCAGC 
TTACAACACA GCCGGGAGGA TTCTTTAGAT CTGAGCCCAC AGGGACGACT GAGTGATGTT 
CTGAAGAGAA AAAGACTGCC AAAACGAGGG CCAAGGAGAC CAAAATATTC TCCCCCAAGA 
GATGATGACA AAGTAGACAA TCAAGCAAAA AGCCCCACGA CTACTCAGTC TCCTAAATCT 
TCTTTCTTAG CAAGTTTAAA TCCCAAAACA TGGGGAAGAT TAAGTACACA GTCTAACAGT 
AATAACATTG AACCAGCAAG AACAGCAGGT GTAAGTGGTC TTGCAAGAGC TGCTTCTAAG 
GATACAATTT CCAATAACAG AGAAAAAATT AAGGGTTGGA TTAAGGAGCA AGCCCATAAA 
TTTGTAGACC GTTATTTTAG TTCGGAAAAC ATGGATGGAA GCAATCCTGC ACTAAATGTA 
TTACAGAGAC TTTGCACTGC AACTGAACAA CTTAACCTCC AGGTGGATGG TGGAACAGAG 
TGCCTTGTAG AAATCCGTAG CATTGTCTCG GAGTCTGACG TCTCCTCATT TGAAATCCAG 
CATAGTGGAT TTGTGAAACA ACTGCTGCTT TATTTGACAT CTAAAAGTGA GAAAGATGCT 
GTAAGCAGGG ATATCAGATT GAAAAGATTT CTTCATGTAT TTTTCTCCTC TCCACTTCCT 
GGAGAAGAAC CCCTTGGAAG ATTAGAGCCA TTAGAAAATG CACCTTTGTT GGCATTAGTC 
CATAAAATGA ACAATTGCCT CAGTCAGATG GAGCAGTTTC CAGTGAAAGT GCATGACTTC 
CCCAGTGGAA ATGGAACAGG GAGCAGTTTT TCTCTTAACA GAGGATCCCA AGCTTTAAAA 
TTCTTCAATA CGCATCAGTT AAAATGCCAG CTGCAAAGAC ATCCAGACTG TGCTAATGTG 
AAACAGTGGA AGGGTGGACC TGTGAAGATT GATCCTCTGG CTTTGGTTCA AGCCATTGAA 
AGATATCTTG TTGTTAGAGG TTATGGAAGA GTTAGAGAAG ATGATGAGGA TAGTGATGAT 
GATGGGTCAG ATGAAGAAAT AGATGAATCC TTGGCTGCTC AATTCTTAAA TTCAGGGAAT 
GTGAGACATA GATTGCAGTT TTACATTGGG GATCACTTGC TACCATATAA TATGACTGTA 
TATCAAGCAG TTAAGCTGTA CAGTTTGCAA GCTGAGGAGG AGAGGGAATC GACTGATGAT 
GAAAGCAATC CATTAGGAAG AGCTGGGATT TGGACAAAAA CACATACCAT TTGGTACAAA 
CCTGTACGGG AGGATGAAGA TGGCAACAAA GACTGTGTTG GTGGTAAAAG AGGAAGAGCT 
CAAACTGCTC CCACAAAAAC CTCACCTAGG AATTCTAAAA AGCATGATGA GTTGTGGCAC 
GATGGTGTAT GCCCTTCAGT ATTGAATCCT TTAGAAGTTT ACCTCATATC TACTCCTCCT 
GAAAACATAA CCTTTGAAGA TCCTTCATTA GATGTTATTC TTCTTTTGAG AGTTTTACAT 
GCTATCAGTC GATACTGGTA TTATTTGTAT GATAATGCAA TATGCAAGGA GATTATTCCA 
ACTTCGGAAT TTATCAATAG TAAACTGACA GCAAAAGCAA ACAGACAGCT TCAAGATCCA 
CTGGTAATTA TGACAGGAAA CATACCGACT TGGCTGACAG AGCTTGGAAA AACATGCCCA 
TTTTTCTTTC CATTTGATAC CCGGCAAATG CTGTTTTATG TAACTGCTTT TGATCGTGAT 
CGAGCCATGC AAAGACTACT GGATACTAAT CCAGAAATCA ATCAATCAGA TTCTCAGGAT 
AGCAGAGTGG CACCACGACT GGACAGGAAA AAACGCACTG TGAACAGAGA TGAGCTGTTG 
AAACAGGCAG AATCTGTGAT GCAGGATCTA GGCAGTTCAA GAGCCATGTT GGAAATCCAG 
TATGAGAATG AAGTTGGCAC AGGCCTGGGC CCTACACTAG AGTTCTATGC ACTTGTATCT 
CAGGAACTAC AGAGAGCAGA CTTGGGCCTT TGGAGGGGAG AAGAAGTAAC TTTAGCCAAT 
CCAAAAGGAA GCCAAGAAGG TACCAAGTAT ATTCATAATC TCCAAGGCCT TTTTGCACTT 
CCTTTTGGTA GAACAGCCAA GCCTGCTCAC ATTGCAAAAG TTAAAATGAA GTTCCGCTTT 
CTGGGAAAAC TAATGGCTAA GGCAATCATG GATTTTAGAC TGGTGGATCT TCCTCTTGGA 
CTTCCTTTTT ATAAATGGAT GTTACGCCAG GAAACTTCTT TGACGTCACA TGACTTGTTC 
AGTATTGATC CAGTAGTAGC CAAATCAATA TATCACCTTG AAGATATTGT AAGACAAAAG 
AAAAGACTTG AGCAGGACAA AACACAGACC AAAGAAAGTC TACAGTATGC ATTGGAAACC 
CTGACTATGA ATGGCTGCTC AGTGGAGGAT CTAGGGCTGG ATTTCACACT TCCTGGATTT 
CCTAACATCG AGCTGAAAAA AGGGGGAAAA GATACACCAG TCACCATCCA CAACTTGGAG 
GAGTACCTCA GATTGGTTAT ATTCTGGGCA CTTAACGAAG GTGTTGCCAG ACAGTTTGAT 
TCTTTCAGAG ATGGATTTGA ATCAGTCTTC CCCCTCAGTC ATCTTCAATA CTTTTATCCT 
GAGGAGTTGG AGCAACTCTT ATGTGGCAGC AAAACAGACA CATGGGATGC CAAGACCTTA 
ATGGAATGTT GCAGGCCAGA TCACGGTTAT ACACATGACA GTCGAGCAGT GAAGTATCTC 
TTTGAAATTC TCAGTAGTTT TGATAGTGAG CAGCAGAGAC TGTTTCTTCA GTTTGTGACC 
GGTAGTCCCA GGCTGCCAGT AGGAGGCTTT CGAAGTTTGA ATCCTCCATT GACAATTGTA 
CGGAAGACAT TTGAGTCTAC AGAGAATCCA GATGACTTCT TGCCCTCAGT AATGACTTGT 
GTGAACTATC TCAAATTGCC GGACTATTCA ACTATTGAGA TAATGCGTGA AAAACTCTTG 
ATAGCTGCAA GAGAAGGGCA GCAGTCATTC CATCTTTCCT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015132455.1
CDS	134..6235
Translation	MSNRPNNNPGGSLRRSQRNTAGAQPQDDAAGGRSHLGQAKHKAHSPPESRKSISKTPKVQSNTTSEQSKGHFSKRGCSSSAVLIPQEDSERVSTSEKQKTGQVPKKDNSRGVKRSASPDYSRTNSPSSAKKPKALQHAETSSESNKPHTKSKRRHLDQEQQKSTQLPSTSKAHTRKGGAAGSSRSQKRKRTESTSCIKSRSAVESTGAEEKSAKSSKLASKSVTSAKAGCSTITDSSSSASTSSSSSAVASASSTVPQGARVKQGKDQNKARRSRSASSPSPRRSSRDKEQSKTGGSSKFDWAARFSPKVSLPKTKLSLPGSSKSETSKPGPSGLQAKLASLRKSTKKRSESPPAELPSLRRSTRQKTTGSCASTSRRGSGLGKRGAAEARRQEKMADPDNNQDGVNSSAARTDETPQGAAASSSVAGAVGMTTSGESESDDSEMGRLQGSKAQQLLQGLQATDESQQLQAVIEMCQLLVMGNEETLGGFPVKSVVPALITLLQMEHNFDIMNHACRALTYMMEALPRSSAVVVDAIPVFLEKLQVIQCIDVAEQALTALEMLSRRHSKAILQAGGLADCLLYLEFFSINAQRNALAIAANCCQSITPDEFHFVADSLPLLTQRLTHQDKKSVESTCLCFARLVDNFQHEENLLQQVASKDLLTNIQQLLVVTPPILSSGMFIMVVRMFSLMCSNCPTLAVQLMKQNIAETLHFLLCGASNGSCQEQIDLVPRSPQELYELTSLICELMPCLPKEGIFAVDTMLKKGNAQNTDGAIWQWRDDRGLWHPYNRIDSRIIEAAHQVGEDEISLSTLGRVYTIDFNSMQQINEDTGTARAIQRKPNPLANTNTSGHSELKKDDARAQLMKEDPELAKSFIKTLFGVLYEVYSSSAGPAVRHKCLRAILRIIYFADAELLKDVLKNHAVSSHIASMLSSQDLKIVVGALQMAEILMQKLPDIFSVYFRREGVMHQVKNLAESEALLTSPPKVCTNGSGTLGTTTTISTGTATAATNAAADLGSPSLQHSREDSLDLSPQGRLSDVLKRKRLPKRGPRRPKYSPPRDDDKVDNQAKSPTTTQSPKSSFLASLNPKTWGRLSTQSNSNNIEPARTAGVSGLARAASKDTISNNREKIKGWIKEQAHKFVDRYFSSENMDGSNPALNVLQRLCTATEQLNLQVDGGTECLVEIRSIVSESDVSSFEIQHSGFVKQLLLYLTSKSEKDAVSRDIRLKRFLHVFFSSPLPGEEPLGRLEPLENAPLLALVHKMNNCLSQMEQFPVKVHDFPSGNGTGSSFSLNRGSQALKFFNTHQLKCQLQRHPDCANVKQWKGGPVKIDPLALVQAIERYLVVRGYGRVREDDEDSDDDGSDEEIDESLAAQFLNSGNVRHRLQFYIGDHLLPYNMTVYQAVKLYSLQAEEERESTDDESNPLGRAGIWTKTHTIWYKPVREDEDGNKDCVGGKRGRAQTAPTKTSPRNSKKHDELWHDGVCPSVLNPLEVYLISTPPENITFEDPSLDVILLLRVLHAISRYWYYLYDNAICKEIIPTSEFINSKLTAKANRQLQDPLVIMTGNIPTWLTELGKTCPFFFPFDTRQMLFYVTAFDRDRAMQRLLDTNPEINQSDSQDSRVAPRLDRKKRTVNRDELLKQAESVMQDLGSSRAMLEIQYENEVGTGLGPTLEFYALVSQELQRADLGLWRGEEVTLANPKGSQEGTKYIHNLQGLFALPFGRTAKPAHIAKVKMKFRFLGKLMAKAIMDFRLVDLPLGLPFYKWMLRQETSLTSHDLFSIDPVVAKSIYHLEDIVRQKKRLEQDKTQTKESLQYALETLTMNGCSVEDLGLDFTLPGFPNIELKKGGKDTPVTIHNLEEYLRLVIFWALNEGVARQFDSFRDGFESVFPLSHLQYFYPEELEQLLCGSKTDTWDAKTLMECCRPDHGYTHDSRAVKYLFEILSSFDSEQQRLFLQFVTGSPRLPVGGFRSLNPPLTIVRKTFESTENPDDFLPSVMTCVNYLKLPDYSTIEIMREKLLIAAREGQQSFHLS

Target ORF information:

RefSeq Version	XM_015276969.2
Organism	Gallus gallus(chicken)
Definition	Gallus gallus thyroid hormone receptor interactor 12 (TRIP12), transcript variant X7, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015276969.2

ORF Insert Sequence:

ATGTCCAACC GGCCTAATAA CAATCCAGGG GGGTCACTGC GACGTTCACA GAGGAACACT 
GCTGGGGCCC AGCCACAAGA CGACGCAGCA GGAGGAAGGT CACATTTAGG GCAGGCAAAA 
CATAAGGCAC ATAGCCCTCC TGAGAGTAGA AAATCTATTT CAAAGACACC CAAAGTGCAG 
TCTAATACTA CTTCTGAGCA GTCCAAGGGA CACTTTTCTA AAAGAGGCTG TAGTTCATCT 
GCTGTTTTAA TACCACAAGA AGATTCAGAA AGAGTCAGTA CTTCAGAAAA GCAAAAAACG 
GGGCAAGTGC CTAAGAAAGA CAATTCTCGA GGAGTTAAAC GCAGTGCTAG TCCAGATTAC 
AGCAGGACCA ATTCTCCTAG CTCTGCTAAA AAACCTAAAG CACTTCAACA CGCCGAAACT 
TCCTCGGAAA GTAACAAGCC ACATACTAAA TCCAAGAGAA GACACTTAGA CCAAGAACAG 
CAGAAGTCTA CACAATTGCC ATCAACAAGC AAGGCTCACA CCAGAAAGGG TGGAGCTGCT 
GGTAGCTCCC GAAGTCAGAA AAGGAAAAGG ACAGAGAGTA CGTCTTGTAT AAAGAGTCGT 
TCAGCAGTTG AATCAACTGG AGCTGAAGAG AAGTCAGCAA AATCCTCCAA GCTGGCTTCA 
AAATCGGTGA CCTCAGCCAA AGCTGGGTGT AGCACCATCA CTGATTCTTC TTCTTCAGCT 
TCTACATCCT CTTCCTCTTC TGCTGTTGCC TCTGCTTCTT CCACTGTTCC TCAGGGTGCC 
AGAGTGAAAC AGGGTAAGGA CCAGAATAAG GCTAGACGTT CACGTTCTGC GTCCAGCCCT 
AGTCCAAGAA GGAGCAGCAG GGACAAAGAA CAGAGCAAAA CAGGTGGCTC TTCAAAGTTT 
GACTGGGCTG CTCGATTCAG TCCAAAAGTT AGTCTCCCTA AAACAAAACT GTCTCTACCA 
GGCTCTTCTA AGTCAGAGAC CTCAAAACCT GGACCTTCAG GACTACAGGC TAAGCTAGCA 
AGCCTAAGAA AATCCACAAA GAAGCGCAGT GAATCACCAC CTGCTGAGCT CCCCAGTTTA 
CGGCGGAGCA CACGGCAAAA GACCACGGGC TCCTGTGCGA GTACCAGTCG GCGGGGCTCT 
GGCCTGGGCA AAAGAGGAGC AGCTGAAGCT CGCCGACAGG AGAAGATGGC TGATCCTGAC 
AACAACCAGG ATGGAGTTAA CTCTTCAGCT GCACGTACAG ATGAGACTCC CCAGGGAGCT 
GCAGCTTCTA GCTCTGTTGC TGGGGCTGTA GGTATGACAA CATCTGGAGA AAGTGAGTCA 
GATGACTCTG AGATGGGAAG ACTACAAGGT TCTAAAGCCC AGCAGCTTTT ACAAGGTCTC 
CAAGCCACTG ATGAAAGTCA GCAACTACAG GCAGTTATTG AGATGTGCCA GCTGTTGGTC 
ATGGGAAATG AAGAAACACT GGGAGGATTT CCAGTCAAGA GTGTTGTGCC AGCTTTGATA 
ACATTGCTGC AGATGGAGCA TAACTTTGAC ATCATGAACC ATGCTTGTCG AGCCTTAACA 
TACATGATGG AAGCACTTCC CAGATCATCT GCTGTAGTGG TAGATGCAAT TCCTGTCTTC 
TTGGAAAAGC TGCAAGTTAT TCAGTGCATT GATGTGGCAG AGCAGGCACT GACAGCCCTA 
GAGATGCTAT CACGCAGGCA TAGTAAAGCC ATTCTGCAGG CAGGTGGGTT GGCAGACTGC 
TTGCTGTATC TGGAATTCTT CAGTATAAAT GCGCAGAGGA ATGCACTAGC TATTGCTGCC 
AATTGCTGCC AGAGTATAAC ACCTGATGAG TTTCACTTTG TGGCAGACTC TTTGCCACTG 
CTTACACAAA GGTTAACCCA TCAGGACAAG AAGTCTGTTG AAAGCACTTG TCTCTGTTTT 
GCACGGCTGG TAGACAATTT CCAGCATGAG GAGAACTTGC TGCAGCAGGT TGCTTCCAAG 
GACTTGTTAA CAAACATCCA GCAACTCTTG GTAGTGACAC CTCCTATCCT GAGCTCAGGA 
ATGTTCATCA TGGTGGTGCG CATGTTTTCC TTAATGTGTT CCAATTGTCC AACGCTTGCA 
GTCCAACTTA TGAAGCAAAA CATTGCAGAA ACACTTCACT TTCTCCTTTG TGGAGCCTCA 
AATGGGAGTT GTCAAGAACA AATTGACCTT GTTCCACGAA GTCCTCAAGA ACTCTATGAG 
CTTACTTCTC TTATCTGTGA ACTGATGCCT TGTCTGCCAA AAGAGGGGAT ATTTGCTGTT 
GATACTATGC TAAAGAAAGG AAATGCACAA AATACAGATG GTGCGATATG GCAATGGCGA 
GATGACAGGG GTCTCTGGCA TCCCTATAAC AGGATTGATA GTCGAATAAT AGAGGCAGCT 
CATCAGGTTG GTGAGGATGA GATAAGCCTG TCGACACTTG GACGTGTCTA TACTATTGAT 
TTTAATTCTA TGCAGCAAAT AAATGAGGAT ACTGGAACAG CACGTGCCAT TCAGCGGAAA 
CCAAACCCTT TAGCCAATAC AAACACTAGT GGACATTCAG AATTGAAGAA GGATGATGCT 
CGAGCACAGC TAATGAAAGA AGACCCAGAA TTGGCAAAAT CCTTTATCAA AACACTGTTT 
GGTGTTCTTT ATGAAGTGTA TAGTTCTTCA GCTGGACCTG CTGTTAGACA CAAGTGCCTT 
AGAGCAATAC TTAGAATAAT TTATTTTGCT GATGCTGAAC TCCTGAAGGA TGTGCTGAAA 
AACCATGCTG TGTCAAGTCA CATTGCCTCC ATGCTGTCAA GTCAAGACCT TAAGATAGTA 
GTTGGAGCCC TGCAGATGGC AGAAATCTTA ATGCAGAAGC TACCTGACAT TTTTAGTGTT 
TACTTCAGAA GAGAAGGAGT GATGCATCAA GTGAAAAACT TAGCGGAGTC TGAGGCTTTG 
CTAACGAGCC CACCAAAAGT ATGCACTAAT GGATCAGGAA CACTGGGTAC CACTACAACT 
ATAAGTACTG GAACAGCCAC TGCTGCCACT AATGCAGCTG CAGATTTGGG CTCTCCCAGC 
TTACAACACA GCCGGGAGGA TTCTTTAGAT CTGAGCCCAC AGGGACGACT GAGTGATGTT 
CTGAAGAGAA AAAGACTGCC AAAACGAGGG CCAAGGAGAC CAAAATATTC TCCCCCAAGA 
GATGATGACA AAGTAGACAA TCAAGCAAAA AGCCCCACGA CTACTCAGTC TCCTAAATCT 
TCTTTCTTAG CAAGTTTAAA TCCCAAAACA TGGGGAAGAT TAAGTACACA GTCTAACAGT 
AATAACATTG AACCAGCAAG AACAGCAGGT GTAAGTGGTC TTGCAAGAGC TGCTTCTAAG 
GATACAATTT CCAATAACAG AGAAAAAATT AAGGGTTGGA TTAAGGAGCA AGCCCATAAA 
TTTGTAGACC GTTATTTTAG TTCGGAAAAC ATGGATGGAA GCAATCCTGC ACTAAATGTA 
TTACAGAGAC TTTGCACTGC AACTGAACAA CTTAACCTCC AGGTGGATGG TGGAACAGAG 
TGCCTTGTAG AAATCCGTAG CATTGTCTCG GAGTCTGACG TCTCCTCATT TGAAATCCAG 
CATAGTGGAT TTGTGAAACA ACTGCTGCTT TATTTGACAT CTAAAAGTGA GAAAGATGCT 
GTAAGCAGGG ATATCAGATT GAAAAGATTT CTTCATGTAT TTTTCTCCTC TCCACTTCCT 
GGAGAAGAAC CCCTTGGAAG ATTAGAGCCA TTAGAAAATG CACCTTTGTT GGCATTAGTC 
CATAAAATGA ACAATTGCCT CAGTCAGATG GAGCAGTTTC CAGTGAAAGT GCATGACTTC 
CCCAGTGGAA ATGGAACAGG GAGCAGTTTT TCTCTTAACA GAGGATCCCA AGCTTTAAAA 
TTCTTCAATA CGCATCAGTT AAAATGCCAG CTGCAAAGAC ATCCAGACTG TGCTAATGTG 
AAACAGTGGA AGGGTGGACC TGTGAAGATT GATCCTCTGG CTTTGGTTCA AGCCATTGAA 
AGATATCTTG TTGTTAGAGG TTATGGAAGA GTTAGAGAAG ATGATGAGGA TAGTGATGAT 
GATGGGTCAG ATGAAGAAAT AGATGAATCC TTGGCTGCTC AATTCTTAAA TTCAGGGAAT 
GTGAGACATA GATTGCAGTT TTACATTGGG GATCACTTGC TACCATATAA TATGACTGTA 
TATCAAGCAG TTAAGCTGTA CAGTTTGCAA GCTGAGGAGG AGAGGGAATC GACTGATGAT 
GAAAGCAATC CATTAGGAAG AGCTGGGATT TGGACAAAAA CACATACCAT TTGGTACAAA 
CCTGTACGGG AGGATGAAGA TGGCAACAAA GACTGTGTTG GTGGTAAAAG AGGAAGAGCT 
CAAACTGCTC CCACAAAAAC CTCACCTAGG AATTCTAAAA AGCATGATGA GTTGTGGCAC 
GATGGTGTAT GCCCTTCAGT ATTGAATCCT TTAGAAGTTT ACCTCATATC TACTCCTCCT 
GAAAACATAA CCTTTGAAGA TCCTTCATTA GATGTTATTC TTCTTTTGAG AGTTTTACAT 
GCTATCAGTC GATACTGGTA TTATTTGTAT GATAATGCAA TATGCAAGGA GATTATTCCA 
ACTTCGGAAT TTATCAATAG TAAACTGACA GCAAAAGCAA ACAGACAGCT TCAAGATCCA 
CTGGTAATTA TGACAGGAAA CATACCGACT TGGCTGACAG AGCTTGGAAA AACATGCCCA 
TTTTTCTTTC CATTTGATAC CCGGCAAATG CTGTTTTATG TAACTGCTTT TGATCGTGAT 
CGAGCCATGC AAAGACTACT GGATACTAAT CCAGAAATCA ATCAATCAGA TTCTCAGGAT 
AGCAGAGTGG CACCACGACT GGACAGGAAA AAACGCACTG TGAACAGAGA TGAGCTGTTG 
AAACAGGCAG AATCTGTGAT GCAGGATCTA GGCAGTTCAA GAGCCATGTT GGAAATCCAG 
TATGAGAATG AAGTTGGCAC AGGCCTGGGC CCTACACTAG AGTTCTATGC ACTTGTATCT 
CAGGAACTAC AGAGAGCAGA CTTGGGCCTT TGGAGGGGAG AAGAAGTAAC TTTAGCCAAT 
CCAAAAGGAA GCCAAGAAGG TACCAAGTAT ATTCATAATC TCCAAGGCCT TTTTGCACTT 
CCTTTTGGTA GAACAGCCAA GCCTGCTCAC ATTGCAAAAG TTAAAATGAA GTTCCGCTTT 
CTGGGAAAAC TAATGGCTAA GGCAATCATG GATTTTAGAC TGGTGGATCT TCCTCTTGGA 
CTTCCTTTTT ATAAATGGAT GTTACGCCAG GAAACTTCTT TGACGTCACA TGACTTGTTC 
AGTATTGATC CAGTAGTAGC CAAATCAATA TATCACCTTG AAGATATTGT AAGACAAAAG 
AAAAGACTTG AGCAGGACAA AACACAGACC AAAGAAAGTC TACAGTATGC ATTGGAAACC 
CTGACTATGA ATGGCTGCTC AGTGGAGGAT CTAGGGCTGG ATTTCACACT TCCTGGATTT 
CCTAACATCG AGCTGAAAAA AGGGGGAAAA GATACACCAG TCACCATCCA CAACTTGGAG 
GAGTACCTCA GATTGGTTAT ATTCTGGGCA CTTAACGAAG GTGTTGCCAG ACAGTTTGAT 
TCTTTCAGAG ATGGATTTGA ATCAGTCTTC CCCCTCAGTC ATCTTCAATA CTTTTATCCT 
GAGGAGTTGG AGCAACTCTT ATGTGGCAGC AAAACAGACA CATGGGATGC CAAGACCTTA 
ATGGAATGTT GCAGGCCAGA TCACGGTTAT ACACATGACA GTCGAGCAGT GAAGTATCTC 
TTTGAAATTC TCAGTAGTTT TGATAGTGAG CAGCAGAGAC TGTTTCTTCA GTTTGTGACC 
GGTAGTCCCA GGCTGCCAGT AGGAGGCTTT CGAAGTTTGA ATCCTCCATT GACAATTGTA 
CGGAAGACAT TTGAGTCTAC AGAGAATCCA GATGACTTCT TGCCCTCAGT AATGACTTGT 
GTGAACTATC TCAAATTGCC GGACTATTCA ACTATTGAGA TAATGCGTGA AAAACTCTTG 
ATAGCTGCAA GAGAAGGGCA GCAGTCATTC CATCTTTCCT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TRIP12 ( XM_015276970.2 ) cDNA ORF clone, Gallus gallus(chicken) -> XP_015132456.1 Gallus gallus thyroid hormone receptor interactor 12 (TRIP12), transcript variant X8, mRNA.

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CloneID	OGa39717
Clone ID Related Accession (Same CDS sequence)	XM_015276970.2 , XM_015276970.1
Accession Version	XM_015276970.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6099bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-16
Organism	Gallus gallus(chicken)
Product	E3 ubiquitin-protein ligase TRIP12 isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006096.5) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On May 17, 2018 this sequence version replaced XM_015276970.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Gallus gallus Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCCAACC GGCCTAATAA CAATCCAGGG GGGTCACTGC GACGTTCACA GAGGAACACT 
GCTGGGGCCC AGCCACAAGA CGACGCAGCA GGAGGAAGGT CACATTTAGG GCAGGCAAAA 
CATAAGGCAC ATAGCCCTCC TGAGAGTAGA AAATCTATTT CAAAGACACC CAAAGTGCAG 
TCTAATACTA CTTCTGAGCA GTCCAAGGGA CACTTTTCTA AAAGAGGCTG TAGTTCATCT 
GCTGTTTTAA TACCACAAGA AGATTCAGAA AGAGTCAGTA CTTCAGAAAA GCAAAAAACG 
GGGCAAGTGC CTAAGAAAGA CAATTCTCGA GGAGTTAAAC GCAGTGCTAG TCCAGATTAC 
AGCAGGACCA ATTCTCCTAG CTCTGCTAAA AAACCTAAAG CACTTCAACA CGCCGAAACT 
TCCTCGGAAA GTAACAAGCC ACATACTAAA TCCAAGAGAA GACACTTAGA CCAAGAACAG 
CAGAAGTCTA CACAATTGCC ATCAACAAGC AAGGCTCACA CCAGAAAGGG TGGAGCTGCT 
GGTAGCTCCC GAAGTCAGAA AAGGAAAAGG ACAGAGAGTA CGTCTTGTAT AAAGAGTCGT 
TCAGCAGTTG AATCAACTGG AGCTGAAGAG AAGTCAGCAA AATCCTCCAA GCTGGCTTCA 
AAATCGGTGA CCTCAGCCAA AGCTGGGTGT AGCACCATCA CTGATTCTTC TTCTTCAGCT 
TCTACATCCT CTTCCTCTTC TGCTGTTGCC TCTGCTTCTT CCACTGTTCC TCAGGGTGCC 
AGAGTGAAAC AGGGTAAGGA CCAGAATAAG GCTAGACGTT CACGTTCTGC GTCCAGCCCT 
AGTCCAAGAA GGAGCAGCAG GGACAAAGAA CAGAGCAAAA CAGGTGGCTC TTCAAAGTTT 
GACTGGGCTG CTCGATTCAG TCCAAAAGTT AGTCTCCCTA AAACAAAACT GTCTCTACCA 
GGCTCTTCTA AGTCAGAGAC CTCAAAACCT GGACCTTCAG GACTACAGGC TAAGCTAGCA 
AGCCTAAGAA AATCCACAAA GAAGCGCAGT GAATCACCAC CTGCTGAGCT CCCCAGTTTA 
CGGCGGAGCA CACGGCAAAA GACCACGGGC TCCTGTGCGA GTACCAGTCG GCGGGGCTCT 
GGCCTGGGCA AAAGAGGAGC AGCTGAAGCT CGCCGACAGG AGAAGATGGC TGATCCTGAC 
AACAACCAGG ATGGAGTTAA CTCTTCAGCT GCACGTACAG ATGAGACTCC CCAGGGAGCT 
GCAGCTTCTA GCTCTGTTGC TGGGGCTGTA GGTATGACAA CATCTGGAGA AAGTGAGTCA 
GATGACTCTG AGATGGGAAG ACTACAAGCT CTGTTAGAGG CTAGGGGTCT TCCTCCTCAC 
CTGTTTGGCC CTCTTGGTCC TCGGATGTCG CAGCTCTTCC ACAGGACAAT TGGAAGTGGA 
GCTAGTTCTA AAGCCCAGCA GCTTTTACAA GGTCTCCAAG CCACTGATGA AAGTCAGCAA 
CTACAGGCAG TTATTGAGAT GTGCCAGCTG TTGGTCATGG GAAATGAAGA AACACTGGGA 
GGATTTCCAG TCAAGAGTGT TGTGCCAGCT TTGATAACAT TGCTGCAGAT GGAGCATAAC 
TTTGACATCA TGAACCATGC TTGTCGAGCC TTAACATACA TGATGGAAGC ACTTCCCAGA 
TCATCTGCTG TAGTGGTAGA TGCAATTCCT GTCTTCTTGG AAAAGCTGCA AGTTATTCAG 
TGCATTGATG TGGCAGAGCA GGCACTGACA GCCCTAGAGA TGCTATCACG CAGGCATAGT 
AAAGCCATTC TGCAGGCAGG TGGGTTGGCA GACTGCTTGC TGTATCTGGA ATTCTTCAGT 
ATAAATGCGC AGAGGAATGC ACTAGCTATT GCTGCCAATT GCTGCCAGAG TATAACACCT 
GATGAGTTTC ACTTTGTGGC AGACTCTTTG CCACTGCTTA CACAAAGGTT AACCCATCAG 
GACAAGAAGT CTGTTGAAAG CACTTGTCTC TGTTTTGCAC GGCTGGTAGA CAATTTCCAG 
CATGAGGAGA ACTTGCTGCA GCAGGTTGCT TCCAAGGACT TGTTAACAAA CATCCAGCAA 
CTCTTGGTAG TGACACCTCC TATCCTGAGC TCAGGAATGT TCATCATGGT GGTGCGCATG 
TTTTCCTTAA TGTGTTCCAA TTGTCCAACG CTTGCAGTCC AACTTATGAA GCAAAACATT 
GCAGAAACAC TTCACTTTCT CCTTTGTGGA GCCTCAAATG GGAGTTGTCA AGAACAAATT 
GACCTTGTTC CACGAAGTCC TCAAGAACTC TATGAGCTTA CTTCTCTTAT CTGTGAACTG 
ATGCCTTGTC TGCCAAAAGA GGGGATATTT GCTGTTGATA CTATGCTAAA GAAAGGAAAT 
GCACAAAATA CAGATGGTGC GATATGGCAA TGGCGAGATG ACAGGGGTCT CTGGCATCCC 
TATAACAGGA TTGATAGTCG AATAATAGAG GCAGCTCATC AGGTTGGTGA GGATGAGATA 
AGCCTGTCGA CACTTGGACG TGTCTATACT ATTGATTTTA ATTCTATGCA GCAAATAAAT 
GAGGATACTG GAACAGCACG TGCCATTCAG CGGAAACCAA ACCCTTTAGC CAATACAAAC 
ACTAGTGGAC ATTCAGAATT GAAGAAGGAT GATGCTCGAG CACAGCTAAT GAAAGAAGAC 
CCAGAATTGG CAAAATCCTT TATCAAAACA CTGTTTGGTG TTCTTTATGA AGTGTATAGT 
TCTTCAGCTG GACCTGCTGT TAGACACAAG TGCCTTAGAG CAATACTTAG AATAATTTAT 
TTTGCTGATG CTGAACTCCT GAAGGATGTG CTGAAAAACC ATGCTGTGTC AAGTCACATT 
GCCTCCATGC TGTCAAGTCA AGACCTTAAG ATAGTAGTTG GAGCCCTGCA GATGGCAGAA 
ATCTTAATGC AGAAGCTACC TGACATTTTT AGTGTTTACT TCAGAAGAGA AGGAGTGATG 
CATCAAGTGA AAAACTTAGC GGAGTCTGAG GCTTTGCTAA CGAGCCCACC AAAAGTATGC 
ACTAATGGAT CAGGAACACT GGGTACCACT ACAACTATAA GTACTGGAAC AGCCACTGCT 
GCCACTAATG CAGCTGCAGA TTTGGGCTCT CCCAGCTTAC AACACAGCCG GGAGGATTCT 
TTAGATCTGA GCCCACAGGG ACGACTGAGT GATGTTCTGA AGAGAAAAAG ACTGCCAAAA 
CGAGGGCCAA GGAGACCAAA ATATTCTCCC CCAAGAGATG ATGACAAAGT AGACAATCAA 
GCAAAAAGCC CCACGACTAC TCAGTCTCCT AAATCTTCTT TCTTAGCAAG TTTAAATCCC 
AAAACATGGG GAAGATTAAG TACACAGTCT AACAGTAATA ACATTGAACC AGCAAGAACA 
GCAGGTGTAA GTGGTCTTGC AAGAGCTGCT TCTAAGGATA CAATTTCCAA TAACAGAGAA 
AAAATTAAGG GTTGGATTAA GGAGCAAGCC CATAAATTTG TAGACCGTTA TTTTAGTTCG 
GAAAACATGG ATGGAAGCAA TCCTGCACTA AATGTATTAC AGAGACTTTG CACTGCAACT 
GAACAACTTA ACCTCCAGGT GGATGGTGGA ACAGAGTGCC TTGTAGAAAT CCGTAGCATT 
GTCTCGGAGT CTGACGTCTC CTCATTTGAA ATCCAGCATA GTGGATTTGT GAAACAACTG 
CTGCTTTATT TGACATCTAA AAGTGAGAAA GATGCTGTAA GCAGGGATAT CAGATTGAAA 
AGATTTCTTC ATGTATTTTT CTCCTCTCCA CTTCCTGGAG AAGAACCCCT TGGAAGATTA 
GAGCCATTAG AAAATGCACC TTTGTTGGCA TTAGTCCATA AAATGAACAA TTGCCTCAGT 
CAGATGGAGC AGTTTCCAGT GAAAGTGCAT GACTTCCCCA GTGGAAATGG AACAGGGAGC 
AGTTTTTCTC TTAACAGAGG ATCCCAAGCT TTAAAATTCT TCAATACGCA TCAGTTAAAA 
TGCCAGCTGC AAAGACATCC AGACTGTGCT AATGTGAAAC AGTGGAAGGG TGGACCTGTG 
AAGATTGATC CTCTGGCTTT GGTTCAAGCC ATTGAAAGAT ATCTTGTTGT TAGAGGTTAT 
GGAAGAGTTA GAGAAGATGA TGAGGATAGT GATGATGATG GGTCAGATGA AGAAATAGAT 
GAATCCTTGG CTGCTCAATT CTTAAATTCA GGGAATGTGA GACATAGATT GCAGTTTTAC 
ATTGGGGATC ACTTGCTACC ATATAATATG ACTGTATATC AAGCAGTTAA GCTGTACAGT 
TTGCAAGCTG AGGAGGAGAG GGAATCGACT GATGATGAAA GCAATCCATT AGGAAGAGCT 
GGGATTTGGA CAAAAACACA TACCATTTGG TACAAACCTG TACGGGAGGA TGAAGATGGC 
AACAAAGACT GTGTTGGTGG TAAAAGAGGA AGAGCTCAAA CTGCTCCCAC AAAAACCTCA 
CCTAGGAATT CTAAAAAGCA TGATGAGTTG TGGCACGATG GTGTATGCCC TTCAGTATTG 
AATCCTTTAG AAGTTTACCT CATATCTACT CCTCCTGAAA ACATAACCTT TGAAGATCCT 
TCATTAGATG TTATTCTTCT TTTGAGAGTT TTACATGCTA TCAGTCGATA CTGGTATTAT 
TTGTATGATA ATGCAATATG CAAGGAGATT ATTCCAACTT CGGAATTTAT CAATAGTAAA 
CTGACAGCAA AAGCAAACAG ACAGCTTCAA GATCCACTGG TAATTATGAC AGGAAACATA 
CCGACTTGGC TGACAGAGCT TGGAAAAACA TGCCCATTTT TCTTTCCATT TGATACCCGG 
CAAATGCTGT TTTATGTAAC TGCTTTTGAT CGTGATCGAG CCATGCAAAG ACTACTGGAT 
ACTAATCCAG AAATCAATCA ATCAGATTCT CAGGATAGCA GAGTGGCACC ACGACTGGAC 
AGGAAAAAAG TTGGCACAGG CCTGGGCCCT ACACTAGAGT TCTATGCACT TGTATCTCAG 
GAACTACAGA GAGCAGACTT GGGCCTTTGG AGGGGAGAAG AAGTAACTTT AGCCAATCCA 
AAAGGAAGCC AAGAAGGTAC CAAGTATATT CATAATCTCC AAGGCCTTTT TGCACTTCCT 
TTTGGTAGAA CAGCCAAGCC TGCTCACATT GCAAAAGTTA AAATGAAGTT CCGCTTTCTG 
GGAAAACTAA TGGCTAAGGC AATCATGGAT TTTAGACTGG TGGATCTTCC TCTTGGACTT 
CCTTTTTATA AATGGATGTT ACGCCAGGAA ACTTCTTTGA CGTCACATGA CTTGTTCAGT 
ATTGATCCAG TAGTAGCCAA ATCAATATAT CACCTTGAAG ATATTGTAAG ACAAAAGAAA 
AGACTTGAGC AGGACAAAAC ACAGACCAAA GAAAGTCTAC AGTATGCATT GGAAACCCTG 
ACTATGAATG GCTGCTCAGT GGAGGATCTA GGGCTGGATT TCACACTTCC TGGATTTCCT 
AACATCGAGC TGAAAAAAGG GGGAAAAGAT ACACCAGTCA CCATCCACAA CTTGGAGGAG 
TACCTCAGAT TGGTTATATT CTGGGCACTT AACGAAGGTG TTGCCAGACA GTTTGATTCT 
TTCAGAGATG GATTTGAATC AGTCTTCCCC CTCAGTCATC TTCAATACTT TTATCCTGAG 
GAGTTGGAGC AACTCTTATG TGGCAGCAAA ACAGACACAT GGGATGCCAA GACCTTAATG 
GAATGTTGCA GGCCAGATCA CGGTTATACA CATGACAGTC GAGCAGTGAA GTATCTCTTT 
GAAATTCTCA GTAGTTTTGA TAGTGAGCAG CAGAGACTGT TTCTTCAGTT TGTGACCGGT 
AGTCCCAGGC TGCCAGTAGG AGGCTTTCGA AGTTTGAATC CTCCATTGAC AATTGTACGG 
AAGACATTTG AGTCTACAGA GAATCCAGAT GACTTCTTGC CCTCAGTAAT GACTTGTGTG 
AACTATCTCA AATTGCCGGA CTATTCAACT ATTGAGATAA TGCGTGAAAA ACTCTTGATA 
GCTGCAAGAG AAGGGCAGCA GTCATTCCAT CTTTCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015132456.1
CDS	134..6232
Translation	MSNRPNNNPGGSLRRSQRNTAGAQPQDDAAGGRSHLGQAKHKAHSPPESRKSISKTPKVQSNTTSEQSKGHFSKRGCSSSAVLIPQEDSERVSTSEKQKTGQVPKKDNSRGVKRSASPDYSRTNSPSSAKKPKALQHAETSSESNKPHTKSKRRHLDQEQQKSTQLPSTSKAHTRKGGAAGSSRSQKRKRTESTSCIKSRSAVESTGAEEKSAKSSKLASKSVTSAKAGCSTITDSSSSASTSSSSSAVASASSTVPQGARVKQGKDQNKARRSRSASSPSPRRSSRDKEQSKTGGSSKFDWAARFSPKVSLPKTKLSLPGSSKSETSKPGPSGLQAKLASLRKSTKKRSESPPAELPSLRRSTRQKTTGSCASTSRRGSGLGKRGAAEARRQEKMADPDNNQDGVNSSAARTDETPQGAAASSSVAGAVGMTTSGESESDDSEMGRLQALLEARGLPPHLFGPLGPRMSQLFHRTIGSGASSKAQQLLQGLQATDESQQLQAVIEMCQLLVMGNEETLGGFPVKSVVPALITLLQMEHNFDIMNHACRALTYMMEALPRSSAVVVDAIPVFLEKLQVIQCIDVAEQALTALEMLSRRHSKAILQAGGLADCLLYLEFFSINAQRNALAIAANCCQSITPDEFHFVADSLPLLTQRLTHQDKKSVESTCLCFARLVDNFQHEENLLQQVASKDLLTNIQQLLVVTPPILSSGMFIMVVRMFSLMCSNCPTLAVQLMKQNIAETLHFLLCGASNGSCQEQIDLVPRSPQELYELTSLICELMPCLPKEGIFAVDTMLKKGNAQNTDGAIWQWRDDRGLWHPYNRIDSRIIEAAHQVGEDEISLSTLGRVYTIDFNSMQQINEDTGTARAIQRKPNPLANTNTSGHSELKKDDARAQLMKEDPELAKSFIKTLFGVLYEVYSSSAGPAVRHKCLRAILRIIYFADAELLKDVLKNHAVSSHIASMLSSQDLKIVVGALQMAEILMQKLPDIFSVYFRREGVMHQVKNLAESEALLTSPPKVCTNGSGTLGTTTTISTGTATAATNAAADLGSPSLQHSREDSLDLSPQGRLSDVLKRKRLPKRGPRRPKYSPPRDDDKVDNQAKSPTTTQSPKSSFLASLNPKTWGRLSTQSNSNNIEPARTAGVSGLARAASKDTISNNREKIKGWIKEQAHKFVDRYFSSENMDGSNPALNVLQRLCTATEQLNLQVDGGTECLVEIRSIVSESDVSSFEIQHSGFVKQLLLYLTSKSEKDAVSRDIRLKRFLHVFFSSPLPGEEPLGRLEPLENAPLLALVHKMNNCLSQMEQFPVKVHDFPSGNGTGSSFSLNRGSQALKFFNTHQLKCQLQRHPDCANVKQWKGGPVKIDPLALVQAIERYLVVRGYGRVREDDEDSDDDGSDEEIDESLAAQFLNSGNVRHRLQFYIGDHLLPYNMTVYQAVKLYSLQAEEERESTDDESNPLGRAGIWTKTHTIWYKPVREDEDGNKDCVGGKRGRAQTAPTKTSPRNSKKHDELWHDGVCPSVLNPLEVYLISTPPENITFEDPSLDVILLLRVLHAISRYWYYLYDNAICKEIIPTSEFINSKLTAKANRQLQDPLVIMTGNIPTWLTELGKTCPFFFPFDTRQMLFYVTAFDRDRAMQRLLDTNPEINQSDSQDSRVAPRLDRKKVGTGLGPTLEFYALVSQELQRADLGLWRGEEVTLANPKGSQEGTKYIHNLQGLFALPFGRTAKPAHIAKVKMKFRFLGKLMAKAIMDFRLVDLPLGLPFYKWMLRQETSLTSHDLFSIDPVVAKSIYHLEDIVRQKKRLEQDKTQTKESLQYALETLTMNGCSVEDLGLDFTLPGFPNIELKKGGKDTPVTIHNLEEYLRLVIFWALNEGVARQFDSFRDGFESVFPLSHLQYFYPEELEQLLCGSKTDTWDAKTLMECCRPDHGYTHDSRAVKYLFEILSSFDSEQQRLFLQFVTGSPRLPVGGFRSLNPPLTIVRKTFESTENPDDFLPSVMTCVNYLKLPDYSTIEIMREKLLIAAREGQQSFHLS

Target ORF information:

RefSeq Version	XM_015276970.2
Organism	Gallus gallus(chicken)
Definition	Gallus gallus thyroid hormone receptor interactor 12 (TRIP12), transcript variant X8, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015276970.2

ORF Insert Sequence:

ATGTCCAACC GGCCTAATAA CAATCCAGGG GGGTCACTGC GACGTTCACA GAGGAACACT 
GCTGGGGCCC AGCCACAAGA CGACGCAGCA GGAGGAAGGT CACATTTAGG GCAGGCAAAA 
CATAAGGCAC ATAGCCCTCC TGAGAGTAGA AAATCTATTT CAAAGACACC CAAAGTGCAG 
TCTAATACTA CTTCTGAGCA GTCCAAGGGA CACTTTTCTA AAAGAGGCTG TAGTTCATCT 
GCTGTTTTAA TACCACAAGA AGATTCAGAA AGAGTCAGTA CTTCAGAAAA GCAAAAAACG 
GGGCAAGTGC CTAAGAAAGA CAATTCTCGA GGAGTTAAAC GCAGTGCTAG TCCAGATTAC 
AGCAGGACCA ATTCTCCTAG CTCTGCTAAA AAACCTAAAG CACTTCAACA CGCCGAAACT 
TCCTCGGAAA GTAACAAGCC ACATACTAAA TCCAAGAGAA GACACTTAGA CCAAGAACAG 
CAGAAGTCTA CACAATTGCC ATCAACAAGC AAGGCTCACA CCAGAAAGGG TGGAGCTGCT 
GGTAGCTCCC GAAGTCAGAA AAGGAAAAGG ACAGAGAGTA CGTCTTGTAT AAAGAGTCGT 
TCAGCAGTTG AATCAACTGG AGCTGAAGAG AAGTCAGCAA AATCCTCCAA GCTGGCTTCA 
AAATCGGTGA CCTCAGCCAA AGCTGGGTGT AGCACCATCA CTGATTCTTC TTCTTCAGCT 
TCTACATCCT CTTCCTCTTC TGCTGTTGCC TCTGCTTCTT CCACTGTTCC TCAGGGTGCC 
AGAGTGAAAC AGGGTAAGGA CCAGAATAAG GCTAGACGTT CACGTTCTGC GTCCAGCCCT 
AGTCCAAGAA GGAGCAGCAG GGACAAAGAA CAGAGCAAAA CAGGTGGCTC TTCAAAGTTT 
GACTGGGCTG CTCGATTCAG TCCAAAAGTT AGTCTCCCTA AAACAAAACT GTCTCTACCA 
GGCTCTTCTA AGTCAGAGAC CTCAAAACCT GGACCTTCAG GACTACAGGC TAAGCTAGCA 
AGCCTAAGAA AATCCACAAA GAAGCGCAGT GAATCACCAC CTGCTGAGCT CCCCAGTTTA 
CGGCGGAGCA CACGGCAAAA GACCACGGGC TCCTGTGCGA GTACCAGTCG GCGGGGCTCT 
GGCCTGGGCA AAAGAGGAGC AGCTGAAGCT CGCCGACAGG AGAAGATGGC TGATCCTGAC 
AACAACCAGG ATGGAGTTAA CTCTTCAGCT GCACGTACAG ATGAGACTCC CCAGGGAGCT 
GCAGCTTCTA GCTCTGTTGC TGGGGCTGTA GGTATGACAA CATCTGGAGA AAGTGAGTCA 
GATGACTCTG AGATGGGAAG ACTACAAGCT CTGTTAGAGG CTAGGGGTCT TCCTCCTCAC 
CTGTTTGGCC CTCTTGGTCC TCGGATGTCG CAGCTCTTCC ACAGGACAAT TGGAAGTGGA 
GCTAGTTCTA AAGCCCAGCA GCTTTTACAA GGTCTCCAAG CCACTGATGA AAGTCAGCAA 
CTACAGGCAG TTATTGAGAT GTGCCAGCTG TTGGTCATGG GAAATGAAGA AACACTGGGA 
GGATTTCCAG TCAAGAGTGT TGTGCCAGCT TTGATAACAT TGCTGCAGAT GGAGCATAAC 
TTTGACATCA TGAACCATGC TTGTCGAGCC TTAACATACA TGATGGAAGC ACTTCCCAGA 
TCATCTGCTG TAGTGGTAGA TGCAATTCCT GTCTTCTTGG AAAAGCTGCA AGTTATTCAG 
TGCATTGATG TGGCAGAGCA GGCACTGACA GCCCTAGAGA TGCTATCACG CAGGCATAGT 
AAAGCCATTC TGCAGGCAGG TGGGTTGGCA GACTGCTTGC TGTATCTGGA ATTCTTCAGT 
ATAAATGCGC AGAGGAATGC ACTAGCTATT GCTGCCAATT GCTGCCAGAG TATAACACCT 
GATGAGTTTC ACTTTGTGGC AGACTCTTTG CCACTGCTTA CACAAAGGTT AACCCATCAG 
GACAAGAAGT CTGTTGAAAG CACTTGTCTC TGTTTTGCAC GGCTGGTAGA CAATTTCCAG 
CATGAGGAGA ACTTGCTGCA GCAGGTTGCT TCCAAGGACT TGTTAACAAA CATCCAGCAA 
CTCTTGGTAG TGACACCTCC TATCCTGAGC TCAGGAATGT TCATCATGGT GGTGCGCATG 
TTTTCCTTAA TGTGTTCCAA TTGTCCAACG CTTGCAGTCC AACTTATGAA GCAAAACATT 
GCAGAAACAC TTCACTTTCT CCTTTGTGGA GCCTCAAATG GGAGTTGTCA AGAACAAATT 
GACCTTGTTC CACGAAGTCC TCAAGAACTC TATGAGCTTA CTTCTCTTAT CTGTGAACTG 
ATGCCTTGTC TGCCAAAAGA GGGGATATTT GCTGTTGATA CTATGCTAAA GAAAGGAAAT 
GCACAAAATA CAGATGGTGC GATATGGCAA TGGCGAGATG ACAGGGGTCT CTGGCATCCC 
TATAACAGGA TTGATAGTCG AATAATAGAG GCAGCTCATC AGGTTGGTGA GGATGAGATA 
AGCCTGTCGA CACTTGGACG TGTCTATACT ATTGATTTTA ATTCTATGCA GCAAATAAAT 
GAGGATACTG GAACAGCACG TGCCATTCAG CGGAAACCAA ACCCTTTAGC CAATACAAAC 
ACTAGTGGAC ATTCAGAATT GAAGAAGGAT GATGCTCGAG CACAGCTAAT GAAAGAAGAC 
CCAGAATTGG CAAAATCCTT TATCAAAACA CTGTTTGGTG TTCTTTATGA AGTGTATAGT 
TCTTCAGCTG GACCTGCTGT TAGACACAAG TGCCTTAGAG CAATACTTAG AATAATTTAT 
TTTGCTGATG CTGAACTCCT GAAGGATGTG CTGAAAAACC ATGCTGTGTC AAGTCACATT 
GCCTCCATGC TGTCAAGTCA AGACCTTAAG ATAGTAGTTG GAGCCCTGCA GATGGCAGAA 
ATCTTAATGC AGAAGCTACC TGACATTTTT AGTGTTTACT TCAGAAGAGA AGGAGTGATG 
CATCAAGTGA AAAACTTAGC GGAGTCTGAG GCTTTGCTAA CGAGCCCACC AAAAGTATGC 
ACTAATGGAT CAGGAACACT GGGTACCACT ACAACTATAA GTACTGGAAC AGCCACTGCT 
GCCACTAATG CAGCTGCAGA TTTGGGCTCT CCCAGCTTAC AACACAGCCG GGAGGATTCT 
TTAGATCTGA GCCCACAGGG ACGACTGAGT GATGTTCTGA AGAGAAAAAG ACTGCCAAAA 
CGAGGGCCAA GGAGACCAAA ATATTCTCCC CCAAGAGATG ATGACAAAGT AGACAATCAA 
GCAAAAAGCC CCACGACTAC TCAGTCTCCT AAATCTTCTT TCTTAGCAAG TTTAAATCCC 
AAAACATGGG GAAGATTAAG TACACAGTCT AACAGTAATA ACATTGAACC AGCAAGAACA 
GCAGGTGTAA GTGGTCTTGC AAGAGCTGCT TCTAAGGATA CAATTTCCAA TAACAGAGAA 
AAAATTAAGG GTTGGATTAA GGAGCAAGCC CATAAATTTG TAGACCGTTA TTTTAGTTCG 
GAAAACATGG ATGGAAGCAA TCCTGCACTA AATGTATTAC AGAGACTTTG CACTGCAACT 
GAACAACTTA ACCTCCAGGT GGATGGTGGA ACAGAGTGCC TTGTAGAAAT CCGTAGCATT 
GTCTCGGAGT CTGACGTCTC CTCATTTGAA ATCCAGCATA GTGGATTTGT GAAACAACTG 
CTGCTTTATT TGACATCTAA AAGTGAGAAA GATGCTGTAA GCAGGGATAT CAGATTGAAA 
AGATTTCTTC ATGTATTTTT CTCCTCTCCA CTTCCTGGAG AAGAACCCCT TGGAAGATTA 
GAGCCATTAG AAAATGCACC TTTGTTGGCA TTAGTCCATA AAATGAACAA TTGCCTCAGT 
CAGATGGAGC AGTTTCCAGT GAAAGTGCAT GACTTCCCCA GTGGAAATGG AACAGGGAGC 
AGTTTTTCTC TTAACAGAGG ATCCCAAGCT TTAAAATTCT TCAATACGCA TCAGTTAAAA 
TGCCAGCTGC AAAGACATCC AGACTGTGCT AATGTGAAAC AGTGGAAGGG TGGACCTGTG 
AAGATTGATC CTCTGGCTTT GGTTCAAGCC ATTGAAAGAT ATCTTGTTGT TAGAGGTTAT 
GGAAGAGTTA GAGAAGATGA TGAGGATAGT GATGATGATG GGTCAGATGA AGAAATAGAT 
GAATCCTTGG CTGCTCAATT CTTAAATTCA GGGAATGTGA GACATAGATT GCAGTTTTAC 
ATTGGGGATC ACTTGCTACC ATATAATATG ACTGTATATC AAGCAGTTAA GCTGTACAGT 
TTGCAAGCTG AGGAGGAGAG GGAATCGACT GATGATGAAA GCAATCCATT AGGAAGAGCT 
GGGATTTGGA CAAAAACACA TACCATTTGG TACAAACCTG TACGGGAGGA TGAAGATGGC 
AACAAAGACT GTGTTGGTGG TAAAAGAGGA AGAGCTCAAA CTGCTCCCAC AAAAACCTCA 
CCTAGGAATT CTAAAAAGCA TGATGAGTTG TGGCACGATG GTGTATGCCC TTCAGTATTG 
AATCCTTTAG AAGTTTACCT CATATCTACT CCTCCTGAAA ACATAACCTT TGAAGATCCT 
TCATTAGATG TTATTCTTCT TTTGAGAGTT TTACATGCTA TCAGTCGATA CTGGTATTAT 
TTGTATGATA ATGCAATATG CAAGGAGATT ATTCCAACTT CGGAATTTAT CAATAGTAAA 
CTGACAGCAA AAGCAAACAG ACAGCTTCAA GATCCACTGG TAATTATGAC AGGAAACATA 
CCGACTTGGC TGACAGAGCT TGGAAAAACA TGCCCATTTT TCTTTCCATT TGATACCCGG 
CAAATGCTGT TTTATGTAAC TGCTTTTGAT CGTGATCGAG CCATGCAAAG ACTACTGGAT 
ACTAATCCAG AAATCAATCA ATCAGATTCT CAGGATAGCA GAGTGGCACC ACGACTGGAC 
AGGAAAAAAG TTGGCACAGG CCTGGGCCCT ACACTAGAGT TCTATGCACT TGTATCTCAG 
GAACTACAGA GAGCAGACTT GGGCCTTTGG AGGGGAGAAG AAGTAACTTT AGCCAATCCA 
AAAGGAAGCC AAGAAGGTAC CAAGTATATT CATAATCTCC AAGGCCTTTT TGCACTTCCT 
TTTGGTAGAA CAGCCAAGCC TGCTCACATT GCAAAAGTTA AAATGAAGTT CCGCTTTCTG 
GGAAAACTAA TGGCTAAGGC AATCATGGAT TTTAGACTGG TGGATCTTCC TCTTGGACTT 
CCTTTTTATA AATGGATGTT ACGCCAGGAA ACTTCTTTGA CGTCACATGA CTTGTTCAGT 
ATTGATCCAG TAGTAGCCAA ATCAATATAT CACCTTGAAG ATATTGTAAG ACAAAAGAAA 
AGACTTGAGC AGGACAAAAC ACAGACCAAA GAAAGTCTAC AGTATGCATT GGAAACCCTG 
ACTATGAATG GCTGCTCAGT GGAGGATCTA GGGCTGGATT TCACACTTCC TGGATTTCCT 
AACATCGAGC TGAAAAAAGG GGGAAAAGAT ACACCAGTCA CCATCCACAA CTTGGAGGAG 
TACCTCAGAT TGGTTATATT CTGGGCACTT AACGAAGGTG TTGCCAGACA GTTTGATTCT 
TTCAGAGATG GATTTGAATC AGTCTTCCCC CTCAGTCATC TTCAATACTT TTATCCTGAG 
GAGTTGGAGC AACTCTTATG TGGCAGCAAA ACAGACACAT GGGATGCCAA GACCTTAATG 
GAATGTTGCA GGCCAGATCA CGGTTATACA CATGACAGTC GAGCAGTGAA GTATCTCTTT 
GAAATTCTCA GTAGTTTTGA TAGTGAGCAG CAGAGACTGT TTCTTCAGTT TGTGACCGGT 
AGTCCCAGGC TGCCAGTAGG AGGCTTTCGA AGTTTGAATC CTCCATTGAC AATTGTACGG 
AAGACATTTG AGTCTACAGA GAATCCAGAT GACTTCTTGC CCTCAGTAAT GACTTGTGTG 
AACTATCTCA AATTGCCGGA CTATTCAACT ATTGAGATAA TGCGTGAAAA ACTCTTGATA 
GCTGCAAGAG AAGGGCAGCA GTCATTCCAT CTTTCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TRIP12 ( XM_015276970.1 ) cDNA ORF clone, Gallus gallus(chicken) -> XP_015132456.1 Gallus gallus thyroid hormone receptor interactor 12 (TRIP12), transcript variant X9, mRNA.

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CloneID	OGa39717
Clone ID Related Accession (Same CDS sequence)	XM_015276970.2 , XM_015276970.1
Accession Version	XM_015276970.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6099bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-03
Organism	Gallus gallus(chicken)
Product	E3 ubiquitin-protein ligase TRIP12 isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_001471743.3) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Gallus gallus Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCCAACC GGCCTAATAA CAATCCAGGG GGGTCACTGC GACGTTCACA GAGGAACACT 
GCTGGGGCCC AGCCACAAGA CGACGCAGCA GGAGGAAGGT CACATTTAGG GCAGGCAAAA 
CATAAGGCAC ATAGCCCTCC TGAGAGTAGA AAATCTATTT CAAAGACACC CAAAGTGCAG 
TCTAATACTA CTTCTGAGCA GTCCAAGGGA CACTTTTCTA AAAGAGGCTG TAGTTCATCT 
GCTGTTTTAA TACCACAAGA AGATTCAGAA AGAGTCAGTA CTTCAGAAAA GCAAAAAACG 
GGGCAAGTGC CTAAGAAAGA CAATTCTCGA GGAGTTAAAC GCAGTGCTAG TCCAGATTAC 
AGCAGGACCA ATTCTCCTAG CTCTGCTAAA AAACCTAAAG CACTTCAACA CGCCGAAACT 
TCCTCGGAAA GTAACAAGCC ACATACTAAA TCCAAGAGAA GACACTTAGA CCAAGAACAG 
CAGAAGTCTA CACAATTGCC ATCAACAAGC AAGGCTCACA CCAGAAAGGG TGGAGCTGCT 
GGTAGCTCCC GAAGTCAGAA AAGGAAAAGG ACAGAGAGTA CGTCTTGTAT AAAGAGTCGT 
TCAGCAGTTG AATCAACTGG AGCTGAAGAG AAGTCAGCAA AATCCTCCAA GCTGGCTTCA 
AAATCGGTGA CCTCAGCCAA AGCTGGGTGT AGCACCATCA CTGATTCTTC TTCTTCAGCT 
TCTACATCCT CTTCCTCTTC TGCTGTTGCC TCTGCTTCTT CCACTGTTCC TCAGGGTGCC 
AGAGTGAAAC AGGGTAAGGA CCAGAATAAG GCTAGACGTT CACGTTCTGC GTCCAGCCCT 
AGTCCAAGAA GGAGCAGCAG GGACAAAGAA CAGAGCAAAA CAGGTGGCTC TTCAAAGTTT 
GACTGGGCTG CTCGATTCAG TCCAAAAGTT AGTCTCCCTA AAACAAAACT GTCTCTACCA 
GGCTCTTCTA AGTCAGAGAC CTCAAAACCT GGACCTTCAG GACTACAGGC TAAGCTAGCA 
AGCCTAAGAA AATCCACAAA GAAGCGCAGT GAATCACCAC CTGCTGAGCT CCCCAGTTTA 
CGGCGGAGCA CACGGCAAAA GACCACGGGC TCCTGTGCGA GTACCAGTCG GCGGGGCTCT 
GGCCTGGGCA AAAGAGGAGC AGCTGAAGCT CGCCGACAGG AGAAGATGGC TGATCCTGAC 
AACAACCAGG ATGGAGTTAA CTCTTCAGCT GCACGTACAG ATGAGACTCC CCAGGGAGCT 
GCAGCTTCTA GCTCTGTTGC TGGGGCTGTA GGTATGACAA CATCTGGAGA AAGTGAGTCA 
GATGACTCTG AGATGGGAAG ACTACAAGCT CTGTTAGAGG CTAGGGGTCT TCCTCCTCAC 
CTGTTTGGCC CTCTTGGTCC TCGGATGTCG CAGCTCTTCC ACAGGACAAT TGGAAGTGGA 
GCTAGTTCTA AAGCCCAGCA GCTTTTACAA GGTCTCCAAG CCACTGATGA AAGTCAGCAA 
CTACAGGCAG TTATTGAGAT GTGCCAGCTG TTGGTCATGG GAAATGAAGA AACACTGGGA 
GGATTTCCAG TCAAGAGTGT TGTGCCAGCT TTGATAACAT TGCTGCAGAT GGAGCATAAC 
TTTGACATCA TGAACCATGC TTGTCGAGCC TTAACATACA TGATGGAAGC ACTTCCCAGA 
TCATCTGCTG TAGTGGTAGA TGCAATTCCT GTCTTCTTGG AAAAGCTGCA AGTTATTCAG 
TGCATTGATG TGGCAGAGCA GGCACTGACA GCCCTAGAGA TGCTATCACG CAGGCATAGT 
AAAGCCATTC TGCAGGCAGG TGGGTTGGCA GACTGCTTGC TGTATCTGGA ATTCTTCAGT 
ATAAATGCGC AGAGGAATGC ACTAGCTATT GCTGCCAATT GCTGCCAGAG TATAACACCT 
GATGAGTTTC ACTTTGTGGC AGACTCTTTG CCACTGCTTA CACAAAGGTT AACCCATCAG 
GACAAGAAGT CTGTTGAAAG CACTTGTCTC TGTTTTGCAC GGCTGGTAGA CAATTTCCAG 
CATGAGGAGA ACTTGCTGCA GCAGGTTGCT TCCAAGGACT TGTTAACAAA CATCCAGCAA 
CTCTTGGTAG TGACACCTCC TATCCTGAGC TCAGGAATGT TCATCATGGT GGTGCGCATG 
TTTTCCTTAA TGTGTTCCAA TTGTCCAACG CTTGCAGTCC AACTTATGAA GCAAAACATT 
GCAGAAACAC TTCACTTTCT CCTTTGTGGA GCCTCAAATG GGAGTTGTCA AGAACAAATT 
GACCTTGTTC CACGAAGTCC TCAAGAACTC TATGAGCTTA CTTCTCTTAT CTGTGAACTG 
ATGCCTTGTC TGCCAAAAGA GGGGATATTT GCTGTTGATA CTATGCTAAA GAAAGGAAAT 
GCACAAAATA CAGATGGTGC GATATGGCAA TGGCGAGATG ACAGGGGTCT CTGGCATCCC 
TATAACAGGA TTGATAGTCG AATAATAGAG GCAGCTCATC AGGTTGGTGA GGATGAGATA 
AGCCTGTCGA CACTTGGACG TGTCTATACT ATTGATTTTA ATTCTATGCA GCAAATAAAT 
GAGGATACTG GAACAGCACG TGCCATTCAG CGGAAACCAA ACCCTTTAGC CAATACAAAC 
ACTAGTGGAC ATTCAGAATT GAAGAAGGAT GATGCTCGAG CACAGCTAAT GAAAGAAGAC 
CCAGAATTGG CAAAATCCTT TATCAAAACA CTGTTTGGTG TTCTTTATGA AGTGTATAGT 
TCTTCAGCTG GACCTGCTGT TAGACACAAG TGCCTTAGAG CAATACTTAG AATAATTTAT 
TTTGCTGATG CTGAACTCCT GAAGGATGTG CTGAAAAACC ATGCTGTGTC AAGTCACATT 
GCCTCCATGC TGTCAAGTCA AGACCTTAAG ATAGTAGTTG GAGCCCTGCA GATGGCAGAA 
ATCTTAATGC AGAAGCTACC TGACATTTTT AGTGTTTACT TCAGAAGAGA AGGAGTGATG 
CATCAAGTGA AAAACTTAGC GGAGTCTGAG GCTTTGCTAA CGAGCCCACC AAAAGTATGC 
ACTAATGGAT CAGGAACACT GGGTACCACT ACAACTATAA GTACTGGAAC AGCCACTGCT 
GCCACTAATG CAGCTGCAGA TTTGGGCTCT CCCAGCTTAC AACACAGCCG GGAGGATTCT 
TTAGATCTGA GCCCACAGGG ACGACTGAGT GATGTTCTGA AGAGAAAAAG ACTGCCAAAA 
CGAGGGCCAA GGAGACCAAA ATATTCTCCC CCAAGAGATG ATGACAAAGT AGACAATCAA 
GCAAAAAGCC CCACGACTAC TCAGTCTCCT AAATCTTCTT TCTTAGCAAG TTTAAATCCC 
AAAACATGGG GAAGATTAAG TACACAGTCT AACAGTAATA ACATTGAACC AGCAAGAACA 
GCAGGTGTAA GTGGTCTTGC AAGAGCTGCT TCTAAGGATA CAATTTCCAA TAACAGAGAA 
AAAATTAAGG GTTGGATTAA GGAGCAAGCC CATAAATTTG TAGACCGTTA TTTTAGTTCG 
GAAAACATGG ATGGAAGCAA TCCTGCACTA AATGTATTAC AGAGACTTTG CACTGCAACT 
GAACAACTTA ACCTCCAGGT GGATGGTGGA ACAGAGTGCC TTGTAGAAAT CCGTAGCATT 
GTCTCGGAGT CTGACGTCTC CTCATTTGAA ATCCAGCATA GTGGATTTGT GAAACAACTG 
CTGCTTTATT TGACATCTAA AAGTGAGAAA GATGCTGTAA GCAGGGATAT CAGATTGAAA 
AGATTTCTTC ATGTATTTTT CTCCTCTCCA CTTCCTGGAG AAGAACCCCT TGGAAGATTA 
GAGCCATTAG AAAATGCACC TTTGTTGGCA TTAGTCCATA AAATGAACAA TTGCCTCAGT 
CAGATGGAGC AGTTTCCAGT GAAAGTGCAT GACTTCCCCA GTGGAAATGG AACAGGGAGC 
AGTTTTTCTC TTAACAGAGG ATCCCAAGCT TTAAAATTCT TCAATACGCA TCAGTTAAAA 
TGCCAGCTGC AAAGACATCC AGACTGTGCT AATGTGAAAC AGTGGAAGGG TGGACCTGTG 
AAGATTGATC CTCTGGCTTT GGTTCAAGCC ATTGAAAGAT ATCTTGTTGT TAGAGGTTAT 
GGAAGAGTTA GAGAAGATGA TGAGGATAGT GATGATGATG GGTCAGATGA AGAAATAGAT 
GAATCCTTGG CTGCTCAATT CTTAAATTCA GGGAATGTGA GACATAGATT GCAGTTTTAC 
ATTGGGGATC ACTTGCTACC ATATAATATG ACTGTATATC AAGCAGTTAA GCTGTACAGT 
TTGCAAGCTG AGGAGGAGAG GGAATCGACT GATGATGAAA GCAATCCATT AGGAAGAGCT 
GGGATTTGGA CAAAAACACA TACCATTTGG TACAAACCTG TACGGGAGGA TGAAGATGGC 
AACAAAGACT GTGTTGGTGG TAAAAGAGGA AGAGCTCAAA CTGCTCCCAC AAAAACCTCA 
CCTAGGAATT CTAAAAAGCA TGATGAGTTG TGGCACGATG GTGTATGCCC TTCAGTATTG 
AATCCTTTAG AAGTTTACCT CATATCTACT CCTCCTGAAA ACATAACCTT TGAAGATCCT 
TCATTAGATG TTATTCTTCT TTTGAGAGTT TTACATGCTA TCAGTCGATA CTGGTATTAT 
TTGTATGATA ATGCAATATG CAAGGAGATT ATTCCAACTT CGGAATTTAT CAATAGTAAA 
CTGACAGCAA AAGCAAACAG ACAGCTTCAA GATCCACTGG TAATTATGAC AGGAAACATA 
CCGACTTGGC TGACAGAGCT TGGAAAAACA TGCCCATTTT TCTTTCCATT TGATACCCGG 
CAAATGCTGT TTTATGTAAC TGCTTTTGAT CGTGATCGAG CCATGCAAAG ACTACTGGAT 
ACTAATCCAG AAATCAATCA ATCAGATTCT CAGGATAGCA GAGTGGCACC ACGACTGGAC 
AGGAAAAAAG TTGGCACAGG CCTGGGCCCT ACACTAGAGT TCTATGCACT TGTATCTCAG 
GAACTACAGA GAGCAGACTT GGGCCTTTGG AGGGGAGAAG AAGTAACTTT AGCCAATCCA 
AAAGGAAGCC AAGAAGGTAC CAAGTATATT CATAATCTCC AAGGCCTTTT TGCACTTCCT 
TTTGGTAGAA CAGCCAAGCC TGCTCACATT GCAAAAGTTA AAATGAAGTT CCGCTTTCTG 
GGAAAACTAA TGGCTAAGGC AATCATGGAT TTTAGACTGG TGGATCTTCC TCTTGGACTT 
CCTTTTTATA AATGGATGTT ACGCCAGGAA ACTTCTTTGA CGTCACATGA CTTGTTCAGT 
ATTGATCCAG TAGTAGCCAA ATCAATATAT CACCTTGAAG ATATTGTAAG ACAAAAGAAA 
AGACTTGAGC AGGACAAAAC ACAGACCAAA GAAAGTCTAC AGTATGCATT GGAAACCCTG 
ACTATGAATG GCTGCTCAGT GGAGGATCTA GGGCTGGATT TCACACTTCC TGGATTTCCT 
AACATCGAGC TGAAAAAAGG GGGAAAAGAT ACACCAGTCA CCATCCACAA CTTGGAGGAG 
TACCTCAGAT TGGTTATATT CTGGGCACTT AACGAAGGTG TTGCCAGACA GTTTGATTCT 
TTCAGAGATG GATTTGAATC AGTCTTCCCC CTCAGTCATC TTCAATACTT TTATCCTGAG 
GAGTTGGAGC AACTCTTATG TGGCAGCAAA ACAGACACAT GGGATGCCAA GACCTTAATG 
GAATGTTGCA GGCCAGATCA CGGTTATACA CATGACAGTC GAGCAGTGAA GTATCTCTTT 
GAAATTCTCA GTAGTTTTGA TAGTGAGCAG CAGAGACTGT TTCTTCAGTT TGTGACCGGT 
AGTCCCAGGC TGCCAGTAGG AGGCTTTCGA AGTTTGAATC CTCCATTGAC AATTGTACGG 
AAGACATTTG AGTCTACAGA GAATCCAGAT GACTTCTTGC CCTCAGTAAT GACTTGTGTG 
AACTATCTCA AATTGCCGGA CTATTCAACT ATTGAGATAA TGCGTGAAAA ACTCTTGATA 
GCTGCAAGAG AAGGGCAGCA GTCATTCCAT CTTTCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015132456.1
CDS	151..6249
Translation	MSNRPNNNPGGSLRRSQRNTAGAQPQDDAAGGRSHLGQAKHKAHSPPESRKSISKTPKVQSNTTSEQSKGHFSKRGCSSSAVLIPQEDSERVSTSEKQKTGQVPKKDNSRGVKRSASPDYSRTNSPSSAKKPKALQHAETSSESNKPHTKSKRRHLDQEQQKSTQLPSTSKAHTRKGGAAGSSRSQKRKRTESTSCIKSRSAVESTGAEEKSAKSSKLASKSVTSAKAGCSTITDSSSSASTSSSSSAVASASSTVPQGARVKQGKDQNKARRSRSASSPSPRRSSRDKEQSKTGGSSKFDWAARFSPKVSLPKTKLSLPGSSKSETSKPGPSGLQAKLASLRKSTKKRSESPPAELPSLRRSTRQKTTGSCASTSRRGSGLGKRGAAEARRQEKMADPDNNQDGVNSSAARTDETPQGAAASSSVAGAVGMTTSGESESDDSEMGRLQALLEARGLPPHLFGPLGPRMSQLFHRTIGSGASSKAQQLLQGLQATDESQQLQAVIEMCQLLVMGNEETLGGFPVKSVVPALITLLQMEHNFDIMNHACRALTYMMEALPRSSAVVVDAIPVFLEKLQVIQCIDVAEQALTALEMLSRRHSKAILQAGGLADCLLYLEFFSINAQRNALAIAANCCQSITPDEFHFVADSLPLLTQRLTHQDKKSVESTCLCFARLVDNFQHEENLLQQVASKDLLTNIQQLLVVTPPILSSGMFIMVVRMFSLMCSNCPTLAVQLMKQNIAETLHFLLCGASNGSCQEQIDLVPRSPQELYELTSLICELMPCLPKEGIFAVDTMLKKGNAQNTDGAIWQWRDDRGLWHPYNRIDSRIIEAAHQVGEDEISLSTLGRVYTIDFNSMQQINEDTGTARAIQRKPNPLANTNTSGHSELKKDDARAQLMKEDPELAKSFIKTLFGVLYEVYSSSAGPAVRHKCLRAILRIIYFADAELLKDVLKNHAVSSHIASMLSSQDLKIVVGALQMAEILMQKLPDIFSVYFRREGVMHQVKNLAESEALLTSPPKVCTNGSGTLGTTTTISTGTATAATNAAADLGSPSLQHSREDSLDLSPQGRLSDVLKRKRLPKRGPRRPKYSPPRDDDKVDNQAKSPTTTQSPKSSFLASLNPKTWGRLSTQSNSNNIEPARTAGVSGLARAASKDTISNNREKIKGWIKEQAHKFVDRYFSSENMDGSNPALNVLQRLCTATEQLNLQVDGGTECLVEIRSIVSESDVSSFEIQHSGFVKQLLLYLTSKSEKDAVSRDIRLKRFLHVFFSSPLPGEEPLGRLEPLENAPLLALVHKMNNCLSQMEQFPVKVHDFPSGNGTGSSFSLNRGSQALKFFNTHQLKCQLQRHPDCANVKQWKGGPVKIDPLALVQAIERYLVVRGYGRVREDDEDSDDDGSDEEIDESLAAQFLNSGNVRHRLQFYIGDHLLPYNMTVYQAVKLYSLQAEEERESTDDESNPLGRAGIWTKTHTIWYKPVREDEDGNKDCVGGKRGRAQTAPTKTSPRNSKKHDELWHDGVCPSVLNPLEVYLISTPPENITFEDPSLDVILLLRVLHAISRYWYYLYDNAICKEIIPTSEFINSKLTAKANRQLQDPLVIMTGNIPTWLTELGKTCPFFFPFDTRQMLFYVTAFDRDRAMQRLLDTNPEINQSDSQDSRVAPRLDRKKVGTGLGPTLEFYALVSQELQRADLGLWRGEEVTLANPKGSQEGTKYIHNLQGLFALPFGRTAKPAHIAKVKMKFRFLGKLMAKAIMDFRLVDLPLGLPFYKWMLRQETSLTSHDLFSIDPVVAKSIYHLEDIVRQKKRLEQDKTQTKESLQYALETLTMNGCSVEDLGLDFTLPGFPNIELKKGGKDTPVTIHNLEEYLRLVIFWALNEGVARQFDSFRDGFESVFPLSHLQYFYPEELEQLLCGSKTDTWDAKTLMECCRPDHGYTHDSRAVKYLFEILSSFDSEQQRLFLQFVTGSPRLPVGGFRSLNPPLTIVRKTFESTENPDDFLPSVMTCVNYLKLPDYSTIEIMREKLLIAAREGQQSFHLS

Target ORF information:

RefSeq Version	XM_015276970.1
Organism	Gallus gallus(chicken)
Definition	Gallus gallus thyroid hormone receptor interactor 12 (TRIP12), transcript variant X9, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015276970.1

ORF Insert Sequence:

ATGTCCAACC GGCCTAATAA CAATCCAGGG GGGTCACTGC GACGTTCACA GAGGAACACT 
GCTGGGGCCC AGCCACAAGA CGACGCAGCA GGAGGAAGGT CACATTTAGG GCAGGCAAAA 
CATAAGGCAC ATAGCCCTCC TGAGAGTAGA AAATCTATTT CAAAGACACC CAAAGTGCAG 
TCTAATACTA CTTCTGAGCA GTCCAAGGGA CACTTTTCTA AAAGAGGCTG TAGTTCATCT 
GCTGTTTTAA TACCACAAGA AGATTCAGAA AGAGTCAGTA CTTCAGAAAA GCAAAAAACG 
GGGCAAGTGC CTAAGAAAGA CAATTCTCGA GGAGTTAAAC GCAGTGCTAG TCCAGATTAC 
AGCAGGACCA ATTCTCCTAG CTCTGCTAAA AAACCTAAAG CACTTCAACA CGCCGAAACT 
TCCTCGGAAA GTAACAAGCC ACATACTAAA TCCAAGAGAA GACACTTAGA CCAAGAACAG 
CAGAAGTCTA CACAATTGCC ATCAACAAGC AAGGCTCACA CCAGAAAGGG TGGAGCTGCT 
GGTAGCTCCC GAAGTCAGAA AAGGAAAAGG ACAGAGAGTA CGTCTTGTAT AAAGAGTCGT 
TCAGCAGTTG AATCAACTGG AGCTGAAGAG AAGTCAGCAA AATCCTCCAA GCTGGCTTCA 
AAATCGGTGA CCTCAGCCAA AGCTGGGTGT AGCACCATCA CTGATTCTTC TTCTTCAGCT 
TCTACATCCT CTTCCTCTTC TGCTGTTGCC TCTGCTTCTT CCACTGTTCC TCAGGGTGCC 
AGAGTGAAAC AGGGTAAGGA CCAGAATAAG GCTAGACGTT CACGTTCTGC GTCCAGCCCT 
AGTCCAAGAA GGAGCAGCAG GGACAAAGAA CAGAGCAAAA CAGGTGGCTC TTCAAAGTTT 
GACTGGGCTG CTCGATTCAG TCCAAAAGTT AGTCTCCCTA AAACAAAACT GTCTCTACCA 
GGCTCTTCTA AGTCAGAGAC CTCAAAACCT GGACCTTCAG GACTACAGGC TAAGCTAGCA 
AGCCTAAGAA AATCCACAAA GAAGCGCAGT GAATCACCAC CTGCTGAGCT CCCCAGTTTA 
CGGCGGAGCA CACGGCAAAA GACCACGGGC TCCTGTGCGA GTACCAGTCG GCGGGGCTCT 
GGCCTGGGCA AAAGAGGAGC AGCTGAAGCT CGCCGACAGG AGAAGATGGC TGATCCTGAC 
AACAACCAGG ATGGAGTTAA CTCTTCAGCT GCACGTACAG ATGAGACTCC CCAGGGAGCT 
GCAGCTTCTA GCTCTGTTGC TGGGGCTGTA GGTATGACAA CATCTGGAGA AAGTGAGTCA 
GATGACTCTG AGATGGGAAG ACTACAAGCT CTGTTAGAGG CTAGGGGTCT TCCTCCTCAC 
CTGTTTGGCC CTCTTGGTCC TCGGATGTCG CAGCTCTTCC ACAGGACAAT TGGAAGTGGA 
GCTAGTTCTA AAGCCCAGCA GCTTTTACAA GGTCTCCAAG CCACTGATGA AAGTCAGCAA 
CTACAGGCAG TTATTGAGAT GTGCCAGCTG TTGGTCATGG GAAATGAAGA AACACTGGGA 
GGATTTCCAG TCAAGAGTGT TGTGCCAGCT TTGATAACAT TGCTGCAGAT GGAGCATAAC 
TTTGACATCA TGAACCATGC TTGTCGAGCC TTAACATACA TGATGGAAGC ACTTCCCAGA 
TCATCTGCTG TAGTGGTAGA TGCAATTCCT GTCTTCTTGG AAAAGCTGCA AGTTATTCAG 
TGCATTGATG TGGCAGAGCA GGCACTGACA GCCCTAGAGA TGCTATCACG CAGGCATAGT 
AAAGCCATTC TGCAGGCAGG TGGGTTGGCA GACTGCTTGC TGTATCTGGA ATTCTTCAGT 
ATAAATGCGC AGAGGAATGC ACTAGCTATT GCTGCCAATT GCTGCCAGAG TATAACACCT 
GATGAGTTTC ACTTTGTGGC AGACTCTTTG CCACTGCTTA CACAAAGGTT AACCCATCAG 
GACAAGAAGT CTGTTGAAAG CACTTGTCTC TGTTTTGCAC GGCTGGTAGA CAATTTCCAG 
CATGAGGAGA ACTTGCTGCA GCAGGTTGCT TCCAAGGACT TGTTAACAAA CATCCAGCAA 
CTCTTGGTAG TGACACCTCC TATCCTGAGC TCAGGAATGT TCATCATGGT GGTGCGCATG 
TTTTCCTTAA TGTGTTCCAA TTGTCCAACG CTTGCAGTCC AACTTATGAA GCAAAACATT 
GCAGAAACAC TTCACTTTCT CCTTTGTGGA GCCTCAAATG GGAGTTGTCA AGAACAAATT 
GACCTTGTTC CACGAAGTCC TCAAGAACTC TATGAGCTTA CTTCTCTTAT CTGTGAACTG 
ATGCCTTGTC TGCCAAAAGA GGGGATATTT GCTGTTGATA CTATGCTAAA GAAAGGAAAT 
GCACAAAATA CAGATGGTGC GATATGGCAA TGGCGAGATG ACAGGGGTCT CTGGCATCCC 
TATAACAGGA TTGATAGTCG AATAATAGAG GCAGCTCATC AGGTTGGTGA GGATGAGATA 
AGCCTGTCGA CACTTGGACG TGTCTATACT ATTGATTTTA ATTCTATGCA GCAAATAAAT 
GAGGATACTG GAACAGCACG TGCCATTCAG CGGAAACCAA ACCCTTTAGC CAATACAAAC 
ACTAGTGGAC ATTCAGAATT GAAGAAGGAT GATGCTCGAG CACAGCTAAT GAAAGAAGAC 
CCAGAATTGG CAAAATCCTT TATCAAAACA CTGTTTGGTG TTCTTTATGA AGTGTATAGT 
TCTTCAGCTG GACCTGCTGT TAGACACAAG TGCCTTAGAG CAATACTTAG AATAATTTAT 
TTTGCTGATG CTGAACTCCT GAAGGATGTG CTGAAAAACC ATGCTGTGTC AAGTCACATT 
GCCTCCATGC TGTCAAGTCA AGACCTTAAG ATAGTAGTTG GAGCCCTGCA GATGGCAGAA 
ATCTTAATGC AGAAGCTACC TGACATTTTT AGTGTTTACT TCAGAAGAGA AGGAGTGATG 
CATCAAGTGA AAAACTTAGC GGAGTCTGAG GCTTTGCTAA CGAGCCCACC AAAAGTATGC 
ACTAATGGAT CAGGAACACT GGGTACCACT ACAACTATAA GTACTGGAAC AGCCACTGCT 
GCCACTAATG CAGCTGCAGA TTTGGGCTCT CCCAGCTTAC AACACAGCCG GGAGGATTCT 
TTAGATCTGA GCCCACAGGG ACGACTGAGT GATGTTCTGA AGAGAAAAAG ACTGCCAAAA 
CGAGGGCCAA GGAGACCAAA ATATTCTCCC CCAAGAGATG ATGACAAAGT AGACAATCAA 
GCAAAAAGCC CCACGACTAC TCAGTCTCCT AAATCTTCTT TCTTAGCAAG TTTAAATCCC 
AAAACATGGG GAAGATTAAG TACACAGTCT AACAGTAATA ACATTGAACC AGCAAGAACA 
GCAGGTGTAA GTGGTCTTGC AAGAGCTGCT TCTAAGGATA CAATTTCCAA TAACAGAGAA 
AAAATTAAGG GTTGGATTAA GGAGCAAGCC CATAAATTTG TAGACCGTTA TTTTAGTTCG 
GAAAACATGG ATGGAAGCAA TCCTGCACTA AATGTATTAC AGAGACTTTG CACTGCAACT 
GAACAACTTA ACCTCCAGGT GGATGGTGGA ACAGAGTGCC TTGTAGAAAT CCGTAGCATT 
GTCTCGGAGT CTGACGTCTC CTCATTTGAA ATCCAGCATA GTGGATTTGT GAAACAACTG 
CTGCTTTATT TGACATCTAA AAGTGAGAAA GATGCTGTAA GCAGGGATAT CAGATTGAAA 
AGATTTCTTC ATGTATTTTT CTCCTCTCCA CTTCCTGGAG AAGAACCCCT TGGAAGATTA 
GAGCCATTAG AAAATGCACC TTTGTTGGCA TTAGTCCATA AAATGAACAA TTGCCTCAGT 
CAGATGGAGC AGTTTCCAGT GAAAGTGCAT GACTTCCCCA GTGGAAATGG AACAGGGAGC 
AGTTTTTCTC TTAACAGAGG ATCCCAAGCT TTAAAATTCT TCAATACGCA TCAGTTAAAA 
TGCCAGCTGC AAAGACATCC AGACTGTGCT AATGTGAAAC AGTGGAAGGG TGGACCTGTG 
AAGATTGATC CTCTGGCTTT GGTTCAAGCC ATTGAAAGAT ATCTTGTTGT TAGAGGTTAT 
GGAAGAGTTA GAGAAGATGA TGAGGATAGT GATGATGATG GGTCAGATGA AGAAATAGAT 
GAATCCTTGG CTGCTCAATT CTTAAATTCA GGGAATGTGA GACATAGATT GCAGTTTTAC 
ATTGGGGATC ACTTGCTACC ATATAATATG ACTGTATATC AAGCAGTTAA GCTGTACAGT 
TTGCAAGCTG AGGAGGAGAG GGAATCGACT GATGATGAAA GCAATCCATT AGGAAGAGCT 
GGGATTTGGA CAAAAACACA TACCATTTGG TACAAACCTG TACGGGAGGA TGAAGATGGC 
AACAAAGACT GTGTTGGTGG TAAAAGAGGA AGAGCTCAAA CTGCTCCCAC AAAAACCTCA 
CCTAGGAATT CTAAAAAGCA TGATGAGTTG TGGCACGATG GTGTATGCCC TTCAGTATTG 
AATCCTTTAG AAGTTTACCT CATATCTACT CCTCCTGAAA ACATAACCTT TGAAGATCCT 
TCATTAGATG TTATTCTTCT TTTGAGAGTT TTACATGCTA TCAGTCGATA CTGGTATTAT 
TTGTATGATA ATGCAATATG CAAGGAGATT ATTCCAACTT CGGAATTTAT CAATAGTAAA 
CTGACAGCAA AAGCAAACAG ACAGCTTCAA GATCCACTGG TAATTATGAC AGGAAACATA 
CCGACTTGGC TGACAGAGCT TGGAAAAACA TGCCCATTTT TCTTTCCATT TGATACCCGG 
CAAATGCTGT TTTATGTAAC TGCTTTTGAT CGTGATCGAG CCATGCAAAG ACTACTGGAT 
ACTAATCCAG AAATCAATCA ATCAGATTCT CAGGATAGCA GAGTGGCACC ACGACTGGAC 
AGGAAAAAAG TTGGCACAGG CCTGGGCCCT ACACTAGAGT TCTATGCACT TGTATCTCAG 
GAACTACAGA GAGCAGACTT GGGCCTTTGG AGGGGAGAAG AAGTAACTTT AGCCAATCCA 
AAAGGAAGCC AAGAAGGTAC CAAGTATATT CATAATCTCC AAGGCCTTTT TGCACTTCCT 
TTTGGTAGAA CAGCCAAGCC TGCTCACATT GCAAAAGTTA AAATGAAGTT CCGCTTTCTG 
GGAAAACTAA TGGCTAAGGC AATCATGGAT TTTAGACTGG TGGATCTTCC TCTTGGACTT 
CCTTTTTATA AATGGATGTT ACGCCAGGAA ACTTCTTTGA CGTCACATGA CTTGTTCAGT 
ATTGATCCAG TAGTAGCCAA ATCAATATAT CACCTTGAAG ATATTGTAAG ACAAAAGAAA 
AGACTTGAGC AGGACAAAAC ACAGACCAAA GAAAGTCTAC AGTATGCATT GGAAACCCTG 
ACTATGAATG GCTGCTCAGT GGAGGATCTA GGGCTGGATT TCACACTTCC TGGATTTCCT 
AACATCGAGC TGAAAAAAGG GGGAAAAGAT ACACCAGTCA CCATCCACAA CTTGGAGGAG 
TACCTCAGAT TGGTTATATT CTGGGCACTT AACGAAGGTG TTGCCAGACA GTTTGATTCT 
TTCAGAGATG GATTTGAATC AGTCTTCCCC CTCAGTCATC TTCAATACTT TTATCCTGAG 
GAGTTGGAGC AACTCTTATG TGGCAGCAAA ACAGACACAT GGGATGCCAA GACCTTAATG 
GAATGTTGCA GGCCAGATCA CGGTTATACA CATGACAGTC GAGCAGTGAA GTATCTCTTT 
GAAATTCTCA GTAGTTTTGA TAGTGAGCAG CAGAGACTGT TTCTTCAGTT TGTGACCGGT 
AGTCCCAGGC TGCCAGTAGG AGGCTTTCGA AGTTTGAATC CTCCATTGAC AATTGTACGG 
AAGACATTTG AGTCTACAGA GAATCCAGAT GACTTCTTGC CCTCAGTAAT GACTTGTGTG 
AACTATCTCA AATTGCCGGA CTATTCAACT ATTGAGATAA TGCGTGAAAA ACTCTTGATA 
GCTGCAAGAG AAGGGCAGCA GTCATTCCAT CTTTCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TRIP12 ( XM_015276964.1 ) cDNA ORF clone, Gallus gallus(chicken) -> XP_015132450.1 Gallus gallus thyroid hormone receptor interactor 12 (TRIP12), transcript variant X2, mRNA.

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CloneID	OGa12502
Clone ID Related Accession (Same CDS sequence)	XM_015276964.1 , XM_015276968.2 , XM_015276967.1 , XM_015276968.1 , XM_004937064.3 , XM_015276964.2 , XM_015276966.2 , XM_004937064.2 , XM_015276966.1 , XM_015276967.2 , XM_015276965.1
Accession Version	XM_015276964.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6198bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-03
Organism	Gallus gallus(chicken)
Product	E3 ubiquitin-protein ligase TRIP12 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_001471743.3) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Gallus gallus Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCCAACC GGCCTAATAA CAATCCAGGG GGGTCACTGC GACGTTCACA GAGGAACACT 
GCTGGGGCCC AGCCACAAGA CGACGCAGCA GGAGGAAGGT CACATTTAGG GCAGGCAAAA 
CATAAGGCAC ATAGCCCTCC TGAGAGTAGA AAATCTATTT CAAAGACACC CAAAGTGCAG 
TCTAATACTA CTTCTGAGCA GTCCAAGGGA CACTTTTCTA AAAGAGGCTG TAGTTCATCT 
GCTGTTTTAA TACCACAAGA AGATTCAGAA AGAGTCAGTA CTTCAGAAAA GCAAAAAACG 
GGGCAAGTGC CTAAGAAAGA CAATTCTCGA GGAGTTAAAC GCAGTGCTAG TCCAGATTAC 
AGCAGGACCA ATTCTCCTAG CTCTGCTAAA AAACCTAAAG CACTTCAACA CGCCGAAACT 
TCCTCGGAAA GTAACAAGCC ACATACTAAA TCCAAGAGAA GACACTTAGA CCAAGAACAG 
CAGAAGTCTA CACAATTGCC ATCAACAAGC AAGGCTCACA CCAGAAAGGG TGGAGCTGCT 
GGTAGCTCCC GAAGTCAGAA AAGGAAAAGG ACAGAGAGTA CGTCTTGTAT AAAGAGTCGT 
TCAGCAGTTG AATCAACTGG AGCTGAAGAG AAGTCAGCAA AATCCTCCAA GCTGGCTTCA 
AAATCGGTGA CCTCAGCCAA AGCTGGGTGT AGCACCATCA CTGATTCTTC TTCTTCAGCT 
TCTACATCCT CTTCCTCTTC TGCTGTTGCC TCTGCTTCTT CCACTGTTCC TCAGGGTGCC 
AGAGTGAAAC AGGGTAAGGA CCAGAATAAG GCTAGACGTT CACGTTCTGC GTCCAGCCCT 
AGTCCAAGAA GGAGCAGCAG GGACAAAGAA CAGAGCAAAA CAGGTGGCTC TTCAAAGTTT 
GACTGGGCTG CTCGATTCAG TCCAAAAGTT AGTCTCCCTA AAACAAAACT GTCTCTACCA 
GGCTCTTCTA AGTCAGAGAC CTCAAAACCT GGACCTTCAG GACTACAGGC TAAGCTAGCA 
AGCCTAAGAA AATCCACAAA GAAGCGCAGT GAATCACCAC CTGCTGAGCT CCCCAGTTTA 
CGGCGGAGCA CACGGCAAAA GACCACGGGC TCCTGTGCGA GTACCAGTCG GCGGGGCTCT 
GGCCTGGGCA AAAGAGGAGC AGCTGAAGCT CGCCGACAGG AGAAGATGGC TGATCCTGAC 
AACAACCAGG ATGGAGTTAA CTCTTCAGCT GCACGTACAG ATGAGACTCC CCAGGGAGCT 
GCAGCTTCTA GCTCTGTTGC TGGGGCTGTA GGTATGACAA CATCTGGAGA AAGTGAGTCA 
GATGACTCTG AGATGGGAAG ACTACAAGCT CTGTTAGAGG CTAGGGGTCT TCCTCCTCAC 
CTGTTTGGCC CTCTTGGTCC TCGGATGTCG CAGCTCTTCC ACAGGACAAT TGGAAGTGGA 
GCTAGTTCTA AAGCCCAGCA GCTTTTACAA GGTCTCCAAG CCACTGATGA AAGTCAGCAA 
CTACAGGCAG TTATTGAGAT GTGCCAGCTG TTGGTCATGG GAAATGAAGA AACACTGGGA 
GGATTTCCAG TCAAGAGTGT TGTGCCAGCT TTGATAACAT TGCTGCAGAT GGAGCATAAC 
TTTGACATCA TGAACCATGC TTGTCGAGCC TTAACATACA TGATGGAAGC ACTTCCCAGA 
TCATCTGCTG TAGTGGTAGA TGCAATTCCT GTCTTCTTGG AAAAGCTGCA AGTTATTCAG 
TGCATTGATG TGGCAGAGCA GGCACTGACA GCCCTAGAGA TGCTATCACG CAGGCATAGT 
AAAGCCATTC TGCAGGCAGG TGGGTTGGCA GACTGCTTGC TGTATCTGGA ATTCTTCAGT 
ATAAATGCGC AGAGGAATGC ACTAGCTATT GCTGCCAATT GCTGCCAGAG TATAACACCT 
GATGAGTTTC ACTTTGTGGC AGACTCTTTG CCACTGCTTA CACAAAGGTT AACCCATCAG 
GACAAGAAGT CTGTTGAAAG CACTTGTCTC TGTTTTGCAC GGCTGGTAGA CAATTTCCAG 
CATGAGGAGA ACTTGCTGCA GCAGGTTGCT TCCAAGGACT TGTTAACAAA CATCCAGCAA 
CTCTTGGTAG TGACACCTCC TATCCTGAGC TCAGGAATGT TCATCATGGT GGTGCGCATG 
TTTTCCTTAA TGTGTTCCAA TTGTCCAACG CTTGCAGTCC AACTTATGAA GCAAAACATT 
GCAGAAACAC TTCACTTTCT CCTTTGTGGA GCCTCAAATG GGAGTTGTCA AGAACAAATT 
GACCTTGTTC CACGAAGTCC TCAAGAACTC TATGAGCTTA CTTCTCTTAT CTGTGAACTG 
ATGCCTTGTC TGCCAAAAGA GGGGATATTT GCTGTTGATA CTATGCTAAA GAAAGGAAAT 
GCACAAAATA CAGATGGTGC GATATGGCAA TGGCGAGATG ACAGGGGTCT CTGGCATCCC 
TATAACAGGA TTGATAGTCG AATAATAGAG GCAGCTCATC AGGTTGGTGA GGATGAGATA 
AGCCTGTCGA CACTTGGACG TGTCTATACT ATTGATTTTA ATTCTATGCA GCAAATAAAT 
GAGGATACTG GAACAGCACG TGCCATTCAG CGGAAACCAA ACCCTTTAGC CAATACAAAC 
ACTAGTGGAC ATTCAGAATT GAAGAAGGAT GATGCTCGAG CACAGCTAAT GAAAGAAGAC 
CCAGAATTGG CAAAATCCTT TATCAAAACA CTGTTTGGTG TTCTTTATGA AGTGTATAGT 
TCTTCAGCTG GACCTGCTGT TAGACACAAG TGCCTTAGAG CAATACTTAG AATAATTTAT 
TTTGCTGATG CTGAACTCCT GAAGGATGTG CTGAAAAACC ATGCTGTGTC AAGTCACATT 
GCCTCCATGC TGTCAAGTCA AGACCTTAAG ATAGTAGTTG GAGCCCTGCA GATGGCAGAA 
ATCTTAATGC AGAAGCTACC TGACATTTTT AGTGTTTACT TCAGAAGAGA AGGAGTGATG 
CATCAAGTGA AAAACTTAGC GGAGTCTGAG GCTTTGCTAA CGAGCCCACC AAAAGTATGC 
ACTAATGGAT CAGGAACACT GGGTACCACT ACAACTATAA GTACTGGAAC AGCCACTGCT 
GCCACTAATG CAGCTGCAGA TTTGGGCTCT CCCAGCTTAC AACACAGCCG GGAGGATTCT 
TTAGATCTGA GCCCACAGGG ACGACTGAGT GATGTTCTGA AGAGAAAAAG ACTGCCAAAA 
CGAGGGCCAA GGAGACCAAA ATATTCTCCC CCAAGAGATG ATGACAAAGT AGACAATCAA 
GCAAAAAGCC CCACGACTAC TCAGTCTCCT AAATCTTCTT TCTTAGCAAG TTTAAATCCC 
AAAACATGGG GAAGATTAAG TACACAGTCT AACAGTAATA ACATTGAACC AGCAAGAACA 
GCAGGTGTAA GTGGTCTTGC AAGAGCTGCT TCTAAGGATA CAATTTCCAA TAACAGAGAA 
AAAATTAAGG GTTGGATTAA GGAGCAAGCC CATAAATTTG TAGACCGTTA TTTTAGTTCG 
GAAAACATGG ATGGAAGCAA TCCTGCACTA AATGTATTAC AGAGACTTTG CACTGCAACT 
GAACAACTTA ACCTCCAGGT GGATGGTGGA ACAGAGTGCC TTGTAGAAAT CCGTAGCATT 
GTCTCGGAGT CTGACGTCTC CTCATTTGAA ATCCAGCATA GTGGATTTGT GAAACAACTG 
CTGCTTTATT TGACATCTAA AAGTGAGAAA GATGCTGTAA GCAGGGATAT CAGATTGAAA 
AGATTTCTTC ATGTATTTTT CTCCTCTCCA CTTCCTGGAG AAGAACCCCT TGGAAGATTA 
GAGCCATTAG AAAATGCACC TTTGTTGGCA TTAGTCCATA AAATGAACAA TTGCCTCAGT 
CAGATGGAGC AGTTTCCAGT GAAAGTGCAT GACTTCCCCA GTGGAAATGG AACAGGGAGC 
AGTTTTTCTC TTAACAGAGG ATCCCAAGCT TTAAAATTCT TCAATACGCA TCAGTTAAAA 
TGCCAGCTGC AAAGACATCC AGACTGTGCT AATGTGAAAC AGTGGAAGGG TGGACCTGTG 
AAGATTGATC CTCTGGCTTT GGTTCAAGCC ATTGAAAGAT ATCTTGTTGT TAGAGGTTAT 
GGAAGAGTTA GAGAAGATGA TGAGGATAGT GATGATGATG GGTCAGATGA AGAAATAGAT 
GAATCCTTGG CTGCTCAATT CTTAAATTCA GGGAATGTGA GACATAGATT GCAGTTTTAC 
ATTGGGGATC ACTTGCTACC ATATAATATG ACTGTATATC AAGCAGTTAA GCTGTACAGT 
TTGCAAGCTG AGGAGGAGAG GGAATCGACT GATGATGAAA GCAATCCATT AGGAAGAGCT 
GGGATTTGGA CAAAAACACA TACCATTTGG TACAAACCTG TACGGGAGGA TGAAGATGGC 
AACAAAGACT GTGTTGGTGG TAAAAGAGGA AGAGCTCAAA CTGCTCCCAC AAAAACCTCA 
CCTAGGAATT CTAAAAAGCA TGATGAGTTG TGGCACGATG GTGTATGCCC TTCAGTATTG 
AATCCTTTAG AAGTTTACCT CATATCTACT CCTCCTGAAA ACATAACCTT TGAAGATCCT 
TCATTAGATG TTATTCTTCT TTTGAGAGTT TTACATGCTA TCAGTCGATA CTGGTATTAT 
TTGTATGATA ATGCAATATG CAAGGAGATT ATTCCAACTT CGGAATTTAT CAATAGTAAA 
CTGACAGCAA AAGCAAACAG ACAGCTTCAA GATCCACTGG TAATTATGAC AGGAAACATA 
CCGACTTGGC TGACAGAGCT TGGAAAAACA TGCCCATTTT TCTTTCCATT TGATACCCGG 
CAAATGCTGT TTTATGTAAC TGCTTTTGAT CGTGATCGAG CCATGCAAAG ACTACTGGAT 
ACTAATCCAG AAATCAATCA ATCAGATTCT CAGGATAGCA GAGTGGCACC ACGACTGGAC 
AGGAAAAAAC GCACTGTGAA CAGAGATGAG CTGTTGAAAC AGGCAGAATC TGTGATGCAG 
GATCTAGGCA GTTCAAGAGC CATGTTGGAA ATCCAGTATG AGAATGAAGT TGGCACAGGC 
CTGGGCCCTA CACTAGAGTT CTATGCACTT GTATCTCAGG AACTACAGAG AGCAGACTTG 
GGCCTTTGGA GGGGAGAAGA AGTAACTTTA GCCAATCCAA AAGGAAGCCA AGAAGGTACC 
AAGTATATTC ATAATCTCCA AGGCCTTTTT GCACTTCCTT TTGGTAGAAC AGCCAAGCCT 
GCTCACATTG CAAAAGTTAA AATGAAGTTC CGCTTTCTGG GAAAACTAAT GGCTAAGGCA 
ATCATGGATT TTAGACTGGT GGATCTTCCT CTTGGACTTC CTTTTTATAA ATGGATGTTA 
CGCCAGGAAA CTTCTTTGAC GTCACATGAC TTGTTCAGTA TTGATCCAGT AGTAGCCAAA 
TCAATATATC ACCTTGAAGA TATTGTAAGA CAAAAGAAAA GACTTGAGCA GGACAAAACA 
CAGACCAAAG AAAGTCTACA GTATGCATTG GAAACCCTGA CTATGAATGG CTGCTCAGTG 
GAGGATCTAG GGCTGGATTT CACACTTCCT GGATTTCCTA ACATCGAGCT GAAAAAAGGG 
GGAAAAGATA CACCAGTCAC CATCCACAAC TTGGAGGAGT ACCTCAGATT GGTTATATTC 
TGGGCACTTA ACGAAGGTGT TGCCAGACAG TTTGATTCTT TCAGAGATGG ATTTGAATCA 
GTCTTCCCCC TCAGTCATCT TCAATACTTT TATCCTGAGG AGTTGGAGCA ACTCTTATGT 
GGCAGCAAAA CAGACACATG GGATGCCAAG ACCTTAATGG AATGTTGCAG GCCAGATCAC 
GGTTATACAC ATGACAGTCG AGCAGTGAAG TATCTCTTTG AAATTCTCAG TAGTTTTGAT 
AGTGAGCAGC AGAGACTGTT TCTTCAGTTT GTGACCGGTA GTCCCAGGCT GCCAGTAGGA 
GGCTTTCGAA GTTTGAATCC TCCATTGACA ATTGTACGGA AGACATTTGA GTCTACAGAG 
AATCCAGATG ACTTCTTGCC CTCAGTAATG ACTTGTGTGA ACTATCTCAA ATTGCCGGAC 
TATTCAACTA TTGAGATAAT GCGTGAAAAA CTCTTGATAG CTGCAAGAGA AGGGCAGCAG 
TCATTCCATC TTTCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015132450.1
CDS	219..6416
Translation	MSNRPNNNPGGSLRRSQRNTAGAQPQDDAAGGRSHLGQAKHKAHSPPESRKSISKTPKVQSNTTSEQSKGHFSKRGCSSSAVLIPQEDSERVSTSEKQKTGQVPKKDNSRGVKRSASPDYSRTNSPSSAKKPKALQHAETSSESNKPHTKSKRRHLDQEQQKSTQLPSTSKAHTRKGGAAGSSRSQKRKRTESTSCIKSRSAVESTGAEEKSAKSSKLASKSVTSAKAGCSTITDSSSSASTSSSSSAVASASSTVPQGARVKQGKDQNKARRSRSASSPSPRRSSRDKEQSKTGGSSKFDWAARFSPKVSLPKTKLSLPGSSKSETSKPGPSGLQAKLASLRKSTKKRSESPPAELPSLRRSTRQKTTGSCASTSRRGSGLGKRGAAEARRQEKMADPDNNQDGVNSSAARTDETPQGAAASSSVAGAVGMTTSGESESDDSEMGRLQALLEARGLPPHLFGPLGPRMSQLFHRTIGSGASSKAQQLLQGLQATDESQQLQAVIEMCQLLVMGNEETLGGFPVKSVVPALITLLQMEHNFDIMNHACRALTYMMEALPRSSAVVVDAIPVFLEKLQVIQCIDVAEQALTALEMLSRRHSKAILQAGGLADCLLYLEFFSINAQRNALAIAANCCQSITPDEFHFVADSLPLLTQRLTHQDKKSVESTCLCFARLVDNFQHEENLLQQVASKDLLTNIQQLLVVTPPILSSGMFIMVVRMFSLMCSNCPTLAVQLMKQNIAETLHFLLCGASNGSCQEQIDLVPRSPQELYELTSLICELMPCLPKEGIFAVDTMLKKGNAQNTDGAIWQWRDDRGLWHPYNRIDSRIIEAAHQVGEDEISLSTLGRVYTIDFNSMQQINEDTGTARAIQRKPNPLANTNTSGHSELKKDDARAQLMKEDPELAKSFIKTLFGVLYEVYSSSAGPAVRHKCLRAILRIIYFADAELLKDVLKNHAVSSHIASMLSSQDLKIVVGALQMAEILMQKLPDIFSVYFRREGVMHQVKNLAESEALLTSPPKVCTNGSGTLGTTTTISTGTATAATNAAADLGSPSLQHSREDSLDLSPQGRLSDVLKRKRLPKRGPRRPKYSPPRDDDKVDNQAKSPTTTQSPKSSFLASLNPKTWGRLSTQSNSNNIEPARTAGVSGLARAASKDTISNNREKIKGWIKEQAHKFVDRYFSSENMDGSNPALNVLQRLCTATEQLNLQVDGGTECLVEIRSIVSESDVSSFEIQHSGFVKQLLLYLTSKSEKDAVSRDIRLKRFLHVFFSSPLPGEEPLGRLEPLENAPLLALVHKMNNCLSQMEQFPVKVHDFPSGNGTGSSFSLNRGSQALKFFNTHQLKCQLQRHPDCANVKQWKGGPVKIDPLALVQAIERYLVVRGYGRVREDDEDSDDDGSDEEIDESLAAQFLNSGNVRHRLQFYIGDHLLPYNMTVYQAVKLYSLQAEEERESTDDESNPLGRAGIWTKTHTIWYKPVREDEDGNKDCVGGKRGRAQTAPTKTSPRNSKKHDELWHDGVCPSVLNPLEVYLISTPPENITFEDPSLDVILLLRVLHAISRYWYYLYDNAICKEIIPTSEFINSKLTAKANRQLQDPLVIMTGNIPTWLTELGKTCPFFFPFDTRQMLFYVTAFDRDRAMQRLLDTNPEINQSDSQDSRVAPRLDRKKRTVNRDELLKQAESVMQDLGSSRAMLEIQYENEVGTGLGPTLEFYALVSQELQRADLGLWRGEEVTLANPKGSQEGTKYIHNLQGLFALPFGRTAKPAHIAKVKMKFRFLGKLMAKAIMDFRLVDLPLGLPFYKWMLRQETSLTSHDLFSIDPVVAKSIYHLEDIVRQKKRLEQDKTQTKESLQYALETLTMNGCSVEDLGLDFTLPGFPNIELKKGGKDTPVTIHNLEEYLRLVIFWALNEGVARQFDSFRDGFESVFPLSHLQYFYPEELEQLLCGSKTDTWDAKTLMECCRPDHGYTHDSRAVKYLFEILSSFDSEQQRLFLQFVTGSPRLPVGGFRSLNPPLTIVRKTFESTENPDDFLPSVMTCVNYLKLPDYSTIEIMREKLLIAAREGQQSFHLS

Target ORF information:

RefSeq Version	XM_015276964.1
Organism	Gallus gallus(chicken)
Definition	Gallus gallus thyroid hormone receptor interactor 12 (TRIP12), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015276964.1

ORF Insert Sequence:

ATGTCCAACC GGCCTAATAA CAATCCAGGG GGGTCACTGC GACGTTCACA GAGGAACACT 
GCTGGGGCCC AGCCACAAGA CGACGCAGCA GGAGGAAGGT CACATTTAGG GCAGGCAAAA 
CATAAGGCAC ATAGCCCTCC TGAGAGTAGA AAATCTATTT CAAAGACACC CAAAGTGCAG 
TCTAATACTA CTTCTGAGCA GTCCAAGGGA CACTTTTCTA AAAGAGGCTG TAGTTCATCT 
GCTGTTTTAA TACCACAAGA AGATTCAGAA AGAGTCAGTA CTTCAGAAAA GCAAAAAACG 
GGGCAAGTGC CTAAGAAAGA CAATTCTCGA GGAGTTAAAC GCAGTGCTAG TCCAGATTAC 
AGCAGGACCA ATTCTCCTAG CTCTGCTAAA AAACCTAAAG CACTTCAACA CGCCGAAACT 
TCCTCGGAAA GTAACAAGCC ACATACTAAA TCCAAGAGAA GACACTTAGA CCAAGAACAG 
CAGAAGTCTA CACAATTGCC ATCAACAAGC AAGGCTCACA CCAGAAAGGG TGGAGCTGCT 
GGTAGCTCCC GAAGTCAGAA AAGGAAAAGG ACAGAGAGTA CGTCTTGTAT AAAGAGTCGT 
TCAGCAGTTG AATCAACTGG AGCTGAAGAG AAGTCAGCAA AATCCTCCAA GCTGGCTTCA 
AAATCGGTGA CCTCAGCCAA AGCTGGGTGT AGCACCATCA CTGATTCTTC TTCTTCAGCT 
TCTACATCCT CTTCCTCTTC TGCTGTTGCC TCTGCTTCTT CCACTGTTCC TCAGGGTGCC 
AGAGTGAAAC AGGGTAAGGA CCAGAATAAG GCTAGACGTT CACGTTCTGC GTCCAGCCCT 
AGTCCAAGAA GGAGCAGCAG GGACAAAGAA CAGAGCAAAA CAGGTGGCTC TTCAAAGTTT 
GACTGGGCTG CTCGATTCAG TCCAAAAGTT AGTCTCCCTA AAACAAAACT GTCTCTACCA 
GGCTCTTCTA AGTCAGAGAC CTCAAAACCT GGACCTTCAG GACTACAGGC TAAGCTAGCA 
AGCCTAAGAA AATCCACAAA GAAGCGCAGT GAATCACCAC CTGCTGAGCT CCCCAGTTTA 
CGGCGGAGCA CACGGCAAAA GACCACGGGC TCCTGTGCGA GTACCAGTCG GCGGGGCTCT 
GGCCTGGGCA AAAGAGGAGC AGCTGAAGCT CGCCGACAGG AGAAGATGGC TGATCCTGAC 
AACAACCAGG ATGGAGTTAA CTCTTCAGCT GCACGTACAG ATGAGACTCC CCAGGGAGCT 
GCAGCTTCTA GCTCTGTTGC TGGGGCTGTA GGTATGACAA CATCTGGAGA AAGTGAGTCA 
GATGACTCTG AGATGGGAAG ACTACAAGCT CTGTTAGAGG CTAGGGGTCT TCCTCCTCAC 
CTGTTTGGCC CTCTTGGTCC TCGGATGTCG CAGCTCTTCC ACAGGACAAT TGGAAGTGGA 
GCTAGTTCTA AAGCCCAGCA GCTTTTACAA GGTCTCCAAG CCACTGATGA AAGTCAGCAA 
CTACAGGCAG TTATTGAGAT GTGCCAGCTG TTGGTCATGG GAAATGAAGA AACACTGGGA 
GGATTTCCAG TCAAGAGTGT TGTGCCAGCT TTGATAACAT TGCTGCAGAT GGAGCATAAC 
TTTGACATCA TGAACCATGC TTGTCGAGCC TTAACATACA TGATGGAAGC ACTTCCCAGA 
TCATCTGCTG TAGTGGTAGA TGCAATTCCT GTCTTCTTGG AAAAGCTGCA AGTTATTCAG 
TGCATTGATG TGGCAGAGCA GGCACTGACA GCCCTAGAGA TGCTATCACG CAGGCATAGT 
AAAGCCATTC TGCAGGCAGG TGGGTTGGCA GACTGCTTGC TGTATCTGGA ATTCTTCAGT 
ATAAATGCGC AGAGGAATGC ACTAGCTATT GCTGCCAATT GCTGCCAGAG TATAACACCT 
GATGAGTTTC ACTTTGTGGC AGACTCTTTG CCACTGCTTA CACAAAGGTT AACCCATCAG 
GACAAGAAGT CTGTTGAAAG CACTTGTCTC TGTTTTGCAC GGCTGGTAGA CAATTTCCAG 
CATGAGGAGA ACTTGCTGCA GCAGGTTGCT TCCAAGGACT TGTTAACAAA CATCCAGCAA 
CTCTTGGTAG TGACACCTCC TATCCTGAGC TCAGGAATGT TCATCATGGT GGTGCGCATG 
TTTTCCTTAA TGTGTTCCAA TTGTCCAACG CTTGCAGTCC AACTTATGAA GCAAAACATT 
GCAGAAACAC TTCACTTTCT CCTTTGTGGA GCCTCAAATG GGAGTTGTCA AGAACAAATT 
GACCTTGTTC CACGAAGTCC TCAAGAACTC TATGAGCTTA CTTCTCTTAT CTGTGAACTG 
ATGCCTTGTC TGCCAAAAGA GGGGATATTT GCTGTTGATA CTATGCTAAA GAAAGGAAAT 
GCACAAAATA CAGATGGTGC GATATGGCAA TGGCGAGATG ACAGGGGTCT CTGGCATCCC 
TATAACAGGA TTGATAGTCG AATAATAGAG GCAGCTCATC AGGTTGGTGA GGATGAGATA 
AGCCTGTCGA CACTTGGACG TGTCTATACT ATTGATTTTA ATTCTATGCA GCAAATAAAT 
GAGGATACTG GAACAGCACG TGCCATTCAG CGGAAACCAA ACCCTTTAGC CAATACAAAC 
ACTAGTGGAC ATTCAGAATT GAAGAAGGAT GATGCTCGAG CACAGCTAAT GAAAGAAGAC 
CCAGAATTGG CAAAATCCTT TATCAAAACA CTGTTTGGTG TTCTTTATGA AGTGTATAGT 
TCTTCAGCTG GACCTGCTGT TAGACACAAG TGCCTTAGAG CAATACTTAG AATAATTTAT 
TTTGCTGATG CTGAACTCCT GAAGGATGTG CTGAAAAACC ATGCTGTGTC AAGTCACATT 
GCCTCCATGC TGTCAAGTCA AGACCTTAAG ATAGTAGTTG GAGCCCTGCA GATGGCAGAA 
ATCTTAATGC AGAAGCTACC TGACATTTTT AGTGTTTACT TCAGAAGAGA AGGAGTGATG 
CATCAAGTGA AAAACTTAGC GGAGTCTGAG GCTTTGCTAA CGAGCCCACC AAAAGTATGC 
ACTAATGGAT CAGGAACACT GGGTACCACT ACAACTATAA GTACTGGAAC AGCCACTGCT 
GCCACTAATG CAGCTGCAGA TTTGGGCTCT CCCAGCTTAC AACACAGCCG GGAGGATTCT 
TTAGATCTGA GCCCACAGGG ACGACTGAGT GATGTTCTGA AGAGAAAAAG ACTGCCAAAA 
CGAGGGCCAA GGAGACCAAA ATATTCTCCC CCAAGAGATG ATGACAAAGT AGACAATCAA 
GCAAAAAGCC CCACGACTAC TCAGTCTCCT AAATCTTCTT TCTTAGCAAG TTTAAATCCC 
AAAACATGGG GAAGATTAAG TACACAGTCT AACAGTAATA ACATTGAACC AGCAAGAACA 
GCAGGTGTAA GTGGTCTTGC AAGAGCTGCT TCTAAGGATA CAATTTCCAA TAACAGAGAA 
AAAATTAAGG GTTGGATTAA GGAGCAAGCC CATAAATTTG TAGACCGTTA TTTTAGTTCG 
GAAAACATGG ATGGAAGCAA TCCTGCACTA AATGTATTAC AGAGACTTTG CACTGCAACT 
GAACAACTTA ACCTCCAGGT GGATGGTGGA ACAGAGTGCC TTGTAGAAAT CCGTAGCATT 
GTCTCGGAGT CTGACGTCTC CTCATTTGAA ATCCAGCATA GTGGATTTGT GAAACAACTG 
CTGCTTTATT TGACATCTAA AAGTGAGAAA GATGCTGTAA GCAGGGATAT CAGATTGAAA 
AGATTTCTTC ATGTATTTTT CTCCTCTCCA CTTCCTGGAG AAGAACCCCT TGGAAGATTA 
GAGCCATTAG AAAATGCACC TTTGTTGGCA TTAGTCCATA AAATGAACAA TTGCCTCAGT 
CAGATGGAGC AGTTTCCAGT GAAAGTGCAT GACTTCCCCA GTGGAAATGG AACAGGGAGC 
AGTTTTTCTC TTAACAGAGG ATCCCAAGCT TTAAAATTCT TCAATACGCA TCAGTTAAAA 
TGCCAGCTGC AAAGACATCC AGACTGTGCT AATGTGAAAC AGTGGAAGGG TGGACCTGTG 
AAGATTGATC CTCTGGCTTT GGTTCAAGCC ATTGAAAGAT ATCTTGTTGT TAGAGGTTAT 
GGAAGAGTTA GAGAAGATGA TGAGGATAGT GATGATGATG GGTCAGATGA AGAAATAGAT 
GAATCCTTGG CTGCTCAATT CTTAAATTCA GGGAATGTGA GACATAGATT GCAGTTTTAC 
ATTGGGGATC ACTTGCTACC ATATAATATG ACTGTATATC AAGCAGTTAA GCTGTACAGT 
TTGCAAGCTG AGGAGGAGAG GGAATCGACT GATGATGAAA GCAATCCATT AGGAAGAGCT 
GGGATTTGGA CAAAAACACA TACCATTTGG TACAAACCTG TACGGGAGGA TGAAGATGGC 
AACAAAGACT GTGTTGGTGG TAAAAGAGGA AGAGCTCAAA CTGCTCCCAC AAAAACCTCA 
CCTAGGAATT CTAAAAAGCA TGATGAGTTG TGGCACGATG GTGTATGCCC TTCAGTATTG 
AATCCTTTAG AAGTTTACCT CATATCTACT CCTCCTGAAA ACATAACCTT TGAAGATCCT 
TCATTAGATG TTATTCTTCT TTTGAGAGTT TTACATGCTA TCAGTCGATA CTGGTATTAT 
TTGTATGATA ATGCAATATG CAAGGAGATT ATTCCAACTT CGGAATTTAT CAATAGTAAA 
CTGACAGCAA AAGCAAACAG ACAGCTTCAA GATCCACTGG TAATTATGAC AGGAAACATA 
CCGACTTGGC TGACAGAGCT TGGAAAAACA TGCCCATTTT TCTTTCCATT TGATACCCGG 
CAAATGCTGT TTTATGTAAC TGCTTTTGAT CGTGATCGAG CCATGCAAAG ACTACTGGAT 
ACTAATCCAG AAATCAATCA ATCAGATTCT CAGGATAGCA GAGTGGCACC ACGACTGGAC 
AGGAAAAAAC GCACTGTGAA CAGAGATGAG CTGTTGAAAC AGGCAGAATC TGTGATGCAG 
GATCTAGGCA GTTCAAGAGC CATGTTGGAA ATCCAGTATG AGAATGAAGT TGGCACAGGC 
CTGGGCCCTA CACTAGAGTT CTATGCACTT GTATCTCAGG AACTACAGAG AGCAGACTTG 
GGCCTTTGGA GGGGAGAAGA AGTAACTTTA GCCAATCCAA AAGGAAGCCA AGAAGGTACC 
AAGTATATTC ATAATCTCCA AGGCCTTTTT GCACTTCCTT TTGGTAGAAC AGCCAAGCCT 
GCTCACATTG CAAAAGTTAA AATGAAGTTC CGCTTTCTGG GAAAACTAAT GGCTAAGGCA 
ATCATGGATT TTAGACTGGT GGATCTTCCT CTTGGACTTC CTTTTTATAA ATGGATGTTA 
CGCCAGGAAA CTTCTTTGAC GTCACATGAC TTGTTCAGTA TTGATCCAGT AGTAGCCAAA 
TCAATATATC ACCTTGAAGA TATTGTAAGA CAAAAGAAAA GACTTGAGCA GGACAAAACA 
CAGACCAAAG AAAGTCTACA GTATGCATTG GAAACCCTGA CTATGAATGG CTGCTCAGTG 
GAGGATCTAG GGCTGGATTT CACACTTCCT GGATTTCCTA ACATCGAGCT GAAAAAAGGG 
GGAAAAGATA CACCAGTCAC CATCCACAAC TTGGAGGAGT ACCTCAGATT GGTTATATTC 
TGGGCACTTA ACGAAGGTGT TGCCAGACAG TTTGATTCTT TCAGAGATGG ATTTGAATCA 
GTCTTCCCCC TCAGTCATCT TCAATACTTT TATCCTGAGG AGTTGGAGCA ACTCTTATGT 
GGCAGCAAAA CAGACACATG GGATGCCAAG ACCTTAATGG AATGTTGCAG GCCAGATCAC 
GGTTATACAC ATGACAGTCG AGCAGTGAAG TATCTCTTTG AAATTCTCAG TAGTTTTGAT 
AGTGAGCAGC AGAGACTGTT TCTTCAGTTT GTGACCGGTA GTCCCAGGCT GCCAGTAGGA 
GGCTTTCGAA GTTTGAATCC TCCATTGACA ATTGTACGGA AGACATTTGA GTCTACAGAG 
AATCCAGATG ACTTCTTGCC CTCAGTAATG ACTTGTGTGA ACTATCTCAA ATTGCCGGAC 
TATTCAACTA TTGAGATAAT GCGTGAAAAA CTCTTGATAG CTGCAAGAGA AGGGCAGCAG 
TCATTCCATC TTTCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TRIP12 ( XM_015276968.2 ) cDNA ORF clone, Gallus gallus(chicken) -> XP_015132454.1 Gallus gallus thyroid hormone receptor interactor 12 (TRIP12), transcript variant X5, mRNA.

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CloneID	OGa12502
Clone ID Related Accession (Same CDS sequence)	XM_015276964.1 , XM_015276968.2 , XM_015276967.1 , XM_015276968.1 , XM_004937064.3 , XM_015276964.2 , XM_015276966.2 , XM_004937064.2 , XM_015276966.1 , XM_015276967.2 , XM_015276965.1
Accession Version	XM_015276968.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6198bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-16
Organism	Gallus gallus(chicken)
Product	E3 ubiquitin-protein ligase TRIP12 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006096.5) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On May 17, 2018 this sequence version replaced XM_015276968.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Gallus gallus Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCCAACC GGCCTAATAA CAATCCAGGG GGGTCACTGC GACGTTCACA GAGGAACACT 
GCTGGGGCCC AGCCACAAGA CGACGCAGCA GGAGGAAGGT CACATTTAGG GCAGGCAAAA 
CATAAGGCAC ATAGCCCTCC TGAGAGTAGA AAATCTATTT CAAAGACACC CAAAGTGCAG 
TCTAATACTA CTTCTGAGCA GTCCAAGGGA CACTTTTCTA AAAGAGGCTG TAGTTCATCT 
GCTGTTTTAA TACCACAAGA AGATTCAGAA AGAGTCAGTA CTTCAGAAAA GCAAAAAACG 
GGGCAAGTGC CTAAGAAAGA CAATTCTCGA GGAGTTAAAC GCAGTGCTAG TCCAGATTAC 
AGCAGGACCA ATTCTCCTAG CTCTGCTAAA AAACCTAAAG CACTTCAACA CGCCGAAACT 
TCCTCGGAAA GTAACAAGCC ACATACTAAA TCCAAGAGAA GACACTTAGA CCAAGAACAG 
CAGAAGTCTA CACAATTGCC ATCAACAAGC AAGGCTCACA CCAGAAAGGG TGGAGCTGCT 
GGTAGCTCCC GAAGTCAGAA AAGGAAAAGG ACAGAGAGTA CGTCTTGTAT AAAGAGTCGT 
TCAGCAGTTG AATCAACTGG AGCTGAAGAG AAGTCAGCAA AATCCTCCAA GCTGGCTTCA 
AAATCGGTGA CCTCAGCCAA AGCTGGGTGT AGCACCATCA CTGATTCTTC TTCTTCAGCT 
TCTACATCCT CTTCCTCTTC TGCTGTTGCC TCTGCTTCTT CCACTGTTCC TCAGGGTGCC 
AGAGTGAAAC AGGGTAAGGA CCAGAATAAG GCTAGACGTT CACGTTCTGC GTCCAGCCCT 
AGTCCAAGAA GGAGCAGCAG GGACAAAGAA CAGAGCAAAA CAGGTGGCTC TTCAAAGTTT 
GACTGGGCTG CTCGATTCAG TCCAAAAGTT AGTCTCCCTA AAACAAAACT GTCTCTACCA 
GGCTCTTCTA AGTCAGAGAC CTCAAAACCT GGACCTTCAG GACTACAGGC TAAGCTAGCA 
AGCCTAAGAA AATCCACAAA GAAGCGCAGT GAATCACCAC CTGCTGAGCT CCCCAGTTTA 
CGGCGGAGCA CACGGCAAAA GACCACGGGC TCCTGTGCGA GTACCAGTCG GCGGGGCTCT 
GGCCTGGGCA AAAGAGGAGC AGCTGAAGCT CGCCGACAGG AGAAGATGGC TGATCCTGAC 
AACAACCAGG ATGGAGTTAA CTCTTCAGCT GCACGTACAG ATGAGACTCC CCAGGGAGCT 
GCAGCTTCTA GCTCTGTTGC TGGGGCTGTA GGTATGACAA CATCTGGAGA AAGTGAGTCA 
GATGACTCTG AGATGGGAAG ACTACAAGCT CTGTTAGAGG CTAGGGGTCT TCCTCCTCAC 
CTGTTTGGCC CTCTTGGTCC TCGGATGTCG CAGCTCTTCC ACAGGACAAT TGGAAGTGGA 
GCTAGTTCTA AAGCCCAGCA GCTTTTACAA GGTCTCCAAG CCACTGATGA AAGTCAGCAA 
CTACAGGCAG TTATTGAGAT GTGCCAGCTG TTGGTCATGG GAAATGAAGA AACACTGGGA 
GGATTTCCAG TCAAGAGTGT TGTGCCAGCT TTGATAACAT TGCTGCAGAT GGAGCATAAC 
TTTGACATCA TGAACCATGC TTGTCGAGCC TTAACATACA TGATGGAAGC ACTTCCCAGA 
TCATCTGCTG TAGTGGTAGA TGCAATTCCT GTCTTCTTGG AAAAGCTGCA AGTTATTCAG 
TGCATTGATG TGGCAGAGCA GGCACTGACA GCCCTAGAGA TGCTATCACG CAGGCATAGT 
AAAGCCATTC TGCAGGCAGG TGGGTTGGCA GACTGCTTGC TGTATCTGGA ATTCTTCAGT 
ATAAATGCGC AGAGGAATGC ACTAGCTATT GCTGCCAATT GCTGCCAGAG TATAACACCT 
GATGAGTTTC ACTTTGTGGC AGACTCTTTG CCACTGCTTA CACAAAGGTT AACCCATCAG 
GACAAGAAGT CTGTTGAAAG CACTTGTCTC TGTTTTGCAC GGCTGGTAGA CAATTTCCAG 
CATGAGGAGA ACTTGCTGCA GCAGGTTGCT TCCAAGGACT TGTTAACAAA CATCCAGCAA 
CTCTTGGTAG TGACACCTCC TATCCTGAGC TCAGGAATGT TCATCATGGT GGTGCGCATG 
TTTTCCTTAA TGTGTTCCAA TTGTCCAACG CTTGCAGTCC AACTTATGAA GCAAAACATT 
GCAGAAACAC TTCACTTTCT CCTTTGTGGA GCCTCAAATG GGAGTTGTCA AGAACAAATT 
GACCTTGTTC CACGAAGTCC TCAAGAACTC TATGAGCTTA CTTCTCTTAT CTGTGAACTG 
ATGCCTTGTC TGCCAAAAGA GGGGATATTT GCTGTTGATA CTATGCTAAA GAAAGGAAAT 
GCACAAAATA CAGATGGTGC GATATGGCAA TGGCGAGATG ACAGGGGTCT CTGGCATCCC 
TATAACAGGA TTGATAGTCG AATAATAGAG GCAGCTCATC AGGTTGGTGA GGATGAGATA 
AGCCTGTCGA CACTTGGACG TGTCTATACT ATTGATTTTA ATTCTATGCA GCAAATAAAT 
GAGGATACTG GAACAGCACG TGCCATTCAG CGGAAACCAA ACCCTTTAGC CAATACAAAC 
ACTAGTGGAC ATTCAGAATT GAAGAAGGAT GATGCTCGAG CACAGCTAAT GAAAGAAGAC 
CCAGAATTGG CAAAATCCTT TATCAAAACA CTGTTTGGTG TTCTTTATGA AGTGTATAGT 
TCTTCAGCTG GACCTGCTGT TAGACACAAG TGCCTTAGAG CAATACTTAG AATAATTTAT 
TTTGCTGATG CTGAACTCCT GAAGGATGTG CTGAAAAACC ATGCTGTGTC AAGTCACATT 
GCCTCCATGC TGTCAAGTCA AGACCTTAAG ATAGTAGTTG GAGCCCTGCA GATGGCAGAA 
ATCTTAATGC AGAAGCTACC TGACATTTTT AGTGTTTACT TCAGAAGAGA AGGAGTGATG 
CATCAAGTGA AAAACTTAGC GGAGTCTGAG GCTTTGCTAA CGAGCCCACC AAAAGTATGC 
ACTAATGGAT CAGGAACACT GGGTACCACT ACAACTATAA GTACTGGAAC AGCCACTGCT 
GCCACTAATG CAGCTGCAGA TTTGGGCTCT CCCAGCTTAC AACACAGCCG GGAGGATTCT 
TTAGATCTGA GCCCACAGGG ACGACTGAGT GATGTTCTGA AGAGAAAAAG ACTGCCAAAA 
CGAGGGCCAA GGAGACCAAA ATATTCTCCC CCAAGAGATG ATGACAAAGT AGACAATCAA 
GCAAAAAGCC CCACGACTAC TCAGTCTCCT AAATCTTCTT TCTTAGCAAG TTTAAATCCC 
AAAACATGGG GAAGATTAAG TACACAGTCT AACAGTAATA ACATTGAACC AGCAAGAACA 
GCAGGTGTAA GTGGTCTTGC AAGAGCTGCT TCTAAGGATA CAATTTCCAA TAACAGAGAA 
AAAATTAAGG GTTGGATTAA GGAGCAAGCC CATAAATTTG TAGACCGTTA TTTTAGTTCG 
GAAAACATGG ATGGAAGCAA TCCTGCACTA AATGTATTAC AGAGACTTTG CACTGCAACT 
GAACAACTTA ACCTCCAGGT GGATGGTGGA ACAGAGTGCC TTGTAGAAAT CCGTAGCATT 
GTCTCGGAGT CTGACGTCTC CTCATTTGAA ATCCAGCATA GTGGATTTGT GAAACAACTG 
CTGCTTTATT TGACATCTAA AAGTGAGAAA GATGCTGTAA GCAGGGATAT CAGATTGAAA 
AGATTTCTTC ATGTATTTTT CTCCTCTCCA CTTCCTGGAG AAGAACCCCT TGGAAGATTA 
GAGCCATTAG AAAATGCACC TTTGTTGGCA TTAGTCCATA AAATGAACAA TTGCCTCAGT 
CAGATGGAGC AGTTTCCAGT GAAAGTGCAT GACTTCCCCA GTGGAAATGG AACAGGGAGC 
AGTTTTTCTC TTAACAGAGG ATCCCAAGCT TTAAAATTCT TCAATACGCA TCAGTTAAAA 
TGCCAGCTGC AAAGACATCC AGACTGTGCT AATGTGAAAC AGTGGAAGGG TGGACCTGTG 
AAGATTGATC CTCTGGCTTT GGTTCAAGCC ATTGAAAGAT ATCTTGTTGT TAGAGGTTAT 
GGAAGAGTTA GAGAAGATGA TGAGGATAGT GATGATGATG GGTCAGATGA AGAAATAGAT 
GAATCCTTGG CTGCTCAATT CTTAAATTCA GGGAATGTGA GACATAGATT GCAGTTTTAC 
ATTGGGGATC ACTTGCTACC ATATAATATG ACTGTATATC AAGCAGTTAA GCTGTACAGT 
TTGCAAGCTG AGGAGGAGAG GGAATCGACT GATGATGAAA GCAATCCATT AGGAAGAGCT 
GGGATTTGGA CAAAAACACA TACCATTTGG TACAAACCTG TACGGGAGGA TGAAGATGGC 
AACAAAGACT GTGTTGGTGG TAAAAGAGGA AGAGCTCAAA CTGCTCCCAC AAAAACCTCA 
CCTAGGAATT CTAAAAAGCA TGATGAGTTG TGGCACGATG GTGTATGCCC TTCAGTATTG 
AATCCTTTAG AAGTTTACCT CATATCTACT CCTCCTGAAA ACATAACCTT TGAAGATCCT 
TCATTAGATG TTATTCTTCT TTTGAGAGTT TTACATGCTA TCAGTCGATA CTGGTATTAT 
TTGTATGATA ATGCAATATG CAAGGAGATT ATTCCAACTT CGGAATTTAT CAATAGTAAA 
CTGACAGCAA AAGCAAACAG ACAGCTTCAA GATCCACTGG TAATTATGAC AGGAAACATA 
CCGACTTGGC TGACAGAGCT TGGAAAAACA TGCCCATTTT TCTTTCCATT TGATACCCGG 
CAAATGCTGT TTTATGTAAC TGCTTTTGAT CGTGATCGAG CCATGCAAAG ACTACTGGAT 
ACTAATCCAG AAATCAATCA ATCAGATTCT CAGGATAGCA GAGTGGCACC ACGACTGGAC 
AGGAAAAAAC GCACTGTGAA CAGAGATGAG CTGTTGAAAC AGGCAGAATC TGTGATGCAG 
GATCTAGGCA GTTCAAGAGC CATGTTGGAA ATCCAGTATG AGAATGAAGT TGGCACAGGC 
CTGGGCCCTA CACTAGAGTT CTATGCACTT GTATCTCAGG AACTACAGAG AGCAGACTTG 
GGCCTTTGGA GGGGAGAAGA AGTAACTTTA GCCAATCCAA AAGGAAGCCA AGAAGGTACC 
AAGTATATTC ATAATCTCCA AGGCCTTTTT GCACTTCCTT TTGGTAGAAC AGCCAAGCCT 
GCTCACATTG CAAAAGTTAA AATGAAGTTC CGCTTTCTGG GAAAACTAAT GGCTAAGGCA 
ATCATGGATT TTAGACTGGT GGATCTTCCT CTTGGACTTC CTTTTTATAA ATGGATGTTA 
CGCCAGGAAA CTTCTTTGAC GTCACATGAC TTGTTCAGTA TTGATCCAGT AGTAGCCAAA 
TCAATATATC ACCTTGAAGA TATTGTAAGA CAAAAGAAAA GACTTGAGCA GGACAAAACA 
CAGACCAAAG AAAGTCTACA GTATGCATTG GAAACCCTGA CTATGAATGG CTGCTCAGTG 
GAGGATCTAG GGCTGGATTT CACACTTCCT GGATTTCCTA ACATCGAGCT GAAAAAAGGG 
GGAAAAGATA CACCAGTCAC CATCCACAAC TTGGAGGAGT ACCTCAGATT GGTTATATTC 
TGGGCACTTA ACGAAGGTGT TGCCAGACAG TTTGATTCTT TCAGAGATGG ATTTGAATCA 
GTCTTCCCCC TCAGTCATCT TCAATACTTT TATCCTGAGG AGTTGGAGCA ACTCTTATGT 
GGCAGCAAAA CAGACACATG GGATGCCAAG ACCTTAATGG AATGTTGCAG GCCAGATCAC 
GGTTATACAC ATGACAGTCG AGCAGTGAAG TATCTCTTTG AAATTCTCAG TAGTTTTGAT 
AGTGAGCAGC AGAGACTGTT TCTTCAGTTT GTGACCGGTA GTCCCAGGCT GCCAGTAGGA 
GGCTTTCGAA GTTTGAATCC TCCATTGACA ATTGTACGGA AGACATTTGA GTCTACAGAG 
AATCCAGATG ACTTCTTGCC CTCAGTAATG ACTTGTGTGA ACTATCTCAA ATTGCCGGAC 
TATTCAACTA TTGAGATAAT GCGTGAAAAA CTCTTGATAG CTGCAAGAGA AGGGCAGCAG 
TCATTCCATC TTTCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015132454.1
CDS	71..6268
Translation	MSNRPNNNPGGSLRRSQRNTAGAQPQDDAAGGRSHLGQAKHKAHSPPESRKSISKTPKVQSNTTSEQSKGHFSKRGCSSSAVLIPQEDSERVSTSEKQKTGQVPKKDNSRGVKRSASPDYSRTNSPSSAKKPKALQHAETSSESNKPHTKSKRRHLDQEQQKSTQLPSTSKAHTRKGGAAGSSRSQKRKRTESTSCIKSRSAVESTGAEEKSAKSSKLASKSVTSAKAGCSTITDSSSSASTSSSSSAVASASSTVPQGARVKQGKDQNKARRSRSASSPSPRRSSRDKEQSKTGGSSKFDWAARFSPKVSLPKTKLSLPGSSKSETSKPGPSGLQAKLASLRKSTKKRSESPPAELPSLRRSTRQKTTGSCASTSRRGSGLGKRGAAEARRQEKMADPDNNQDGVNSSAARTDETPQGAAASSSVAGAVGMTTSGESESDDSEMGRLQALLEARGLPPHLFGPLGPRMSQLFHRTIGSGASSKAQQLLQGLQATDESQQLQAVIEMCQLLVMGNEETLGGFPVKSVVPALITLLQMEHNFDIMNHACRALTYMMEALPRSSAVVVDAIPVFLEKLQVIQCIDVAEQALTALEMLSRRHSKAILQAGGLADCLLYLEFFSINAQRNALAIAANCCQSITPDEFHFVADSLPLLTQRLTHQDKKSVESTCLCFARLVDNFQHEENLLQQVASKDLLTNIQQLLVVTPPILSSGMFIMVVRMFSLMCSNCPTLAVQLMKQNIAETLHFLLCGASNGSCQEQIDLVPRSPQELYELTSLICELMPCLPKEGIFAVDTMLKKGNAQNTDGAIWQWRDDRGLWHPYNRIDSRIIEAAHQVGEDEISLSTLGRVYTIDFNSMQQINEDTGTARAIQRKPNPLANTNTSGHSELKKDDARAQLMKEDPELAKSFIKTLFGVLYEVYSSSAGPAVRHKCLRAILRIIYFADAELLKDVLKNHAVSSHIASMLSSQDLKIVVGALQMAEILMQKLPDIFSVYFRREGVMHQVKNLAESEALLTSPPKVCTNGSGTLGTTTTISTGTATAATNAAADLGSPSLQHSREDSLDLSPQGRLSDVLKRKRLPKRGPRRPKYSPPRDDDKVDNQAKSPTTTQSPKSSFLASLNPKTWGRLSTQSNSNNIEPARTAGVSGLARAASKDTISNNREKIKGWIKEQAHKFVDRYFSSENMDGSNPALNVLQRLCTATEQLNLQVDGGTECLVEIRSIVSESDVSSFEIQHSGFVKQLLLYLTSKSEKDAVSRDIRLKRFLHVFFSSPLPGEEPLGRLEPLENAPLLALVHKMNNCLSQMEQFPVKVHDFPSGNGTGSSFSLNRGSQALKFFNTHQLKCQLQRHPDCANVKQWKGGPVKIDPLALVQAIERYLVVRGYGRVREDDEDSDDDGSDEEIDESLAAQFLNSGNVRHRLQFYIGDHLLPYNMTVYQAVKLYSLQAEEERESTDDESNPLGRAGIWTKTHTIWYKPVREDEDGNKDCVGGKRGRAQTAPTKTSPRNSKKHDELWHDGVCPSVLNPLEVYLISTPPENITFEDPSLDVILLLRVLHAISRYWYYLYDNAICKEIIPTSEFINSKLTAKANRQLQDPLVIMTGNIPTWLTELGKTCPFFFPFDTRQMLFYVTAFDRDRAMQRLLDTNPEINQSDSQDSRVAPRLDRKKRTVNRDELLKQAESVMQDLGSSRAMLEIQYENEVGTGLGPTLEFYALVSQELQRADLGLWRGEEVTLANPKGSQEGTKYIHNLQGLFALPFGRTAKPAHIAKVKMKFRFLGKLMAKAIMDFRLVDLPLGLPFYKWMLRQETSLTSHDLFSIDPVVAKSIYHLEDIVRQKKRLEQDKTQTKESLQYALETLTMNGCSVEDLGLDFTLPGFPNIELKKGGKDTPVTIHNLEEYLRLVIFWALNEGVARQFDSFRDGFESVFPLSHLQYFYPEELEQLLCGSKTDTWDAKTLMECCRPDHGYTHDSRAVKYLFEILSSFDSEQQRLFLQFVTGSPRLPVGGFRSLNPPLTIVRKTFESTENPDDFLPSVMTCVNYLKLPDYSTIEIMREKLLIAAREGQQSFHLS

Target ORF information:

RefSeq Version	XM_015276968.2
Organism	Gallus gallus(chicken)
Definition	Gallus gallus thyroid hormone receptor interactor 12 (TRIP12), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015276968.2

ORF Insert Sequence:

ATGTCCAACC GGCCTAATAA CAATCCAGGG GGGTCACTGC GACGTTCACA GAGGAACACT 
GCTGGGGCCC AGCCACAAGA CGACGCAGCA GGAGGAAGGT CACATTTAGG GCAGGCAAAA 
CATAAGGCAC ATAGCCCTCC TGAGAGTAGA AAATCTATTT CAAAGACACC CAAAGTGCAG 
TCTAATACTA CTTCTGAGCA GTCCAAGGGA CACTTTTCTA AAAGAGGCTG TAGTTCATCT 
GCTGTTTTAA TACCACAAGA AGATTCAGAA AGAGTCAGTA CTTCAGAAAA GCAAAAAACG 
GGGCAAGTGC CTAAGAAAGA CAATTCTCGA GGAGTTAAAC GCAGTGCTAG TCCAGATTAC 
AGCAGGACCA ATTCTCCTAG CTCTGCTAAA AAACCTAAAG CACTTCAACA CGCCGAAACT 
TCCTCGGAAA GTAACAAGCC ACATACTAAA TCCAAGAGAA GACACTTAGA CCAAGAACAG 
CAGAAGTCTA CACAATTGCC ATCAACAAGC AAGGCTCACA CCAGAAAGGG TGGAGCTGCT 
GGTAGCTCCC GAAGTCAGAA AAGGAAAAGG ACAGAGAGTA CGTCTTGTAT AAAGAGTCGT 
TCAGCAGTTG AATCAACTGG AGCTGAAGAG AAGTCAGCAA AATCCTCCAA GCTGGCTTCA 
AAATCGGTGA CCTCAGCCAA AGCTGGGTGT AGCACCATCA CTGATTCTTC TTCTTCAGCT 
TCTACATCCT CTTCCTCTTC TGCTGTTGCC TCTGCTTCTT CCACTGTTCC TCAGGGTGCC 
AGAGTGAAAC AGGGTAAGGA CCAGAATAAG GCTAGACGTT CACGTTCTGC GTCCAGCCCT 
AGTCCAAGAA GGAGCAGCAG GGACAAAGAA CAGAGCAAAA CAGGTGGCTC TTCAAAGTTT 
GACTGGGCTG CTCGATTCAG TCCAAAAGTT AGTCTCCCTA AAACAAAACT GTCTCTACCA 
GGCTCTTCTA AGTCAGAGAC CTCAAAACCT GGACCTTCAG GACTACAGGC TAAGCTAGCA 
AGCCTAAGAA AATCCACAAA GAAGCGCAGT GAATCACCAC CTGCTGAGCT CCCCAGTTTA 
CGGCGGAGCA CACGGCAAAA GACCACGGGC TCCTGTGCGA GTACCAGTCG GCGGGGCTCT 
GGCCTGGGCA AAAGAGGAGC AGCTGAAGCT CGCCGACAGG AGAAGATGGC TGATCCTGAC 
AACAACCAGG ATGGAGTTAA CTCTTCAGCT GCACGTACAG ATGAGACTCC CCAGGGAGCT 
GCAGCTTCTA GCTCTGTTGC TGGGGCTGTA GGTATGACAA CATCTGGAGA AAGTGAGTCA 
GATGACTCTG AGATGGGAAG ACTACAAGCT CTGTTAGAGG CTAGGGGTCT TCCTCCTCAC 
CTGTTTGGCC CTCTTGGTCC TCGGATGTCG CAGCTCTTCC ACAGGACAAT TGGAAGTGGA 
GCTAGTTCTA AAGCCCAGCA GCTTTTACAA GGTCTCCAAG CCACTGATGA AAGTCAGCAA 
CTACAGGCAG TTATTGAGAT GTGCCAGCTG TTGGTCATGG GAAATGAAGA AACACTGGGA 
GGATTTCCAG TCAAGAGTGT TGTGCCAGCT TTGATAACAT TGCTGCAGAT GGAGCATAAC 
TTTGACATCA TGAACCATGC TTGTCGAGCC TTAACATACA TGATGGAAGC ACTTCCCAGA 
TCATCTGCTG TAGTGGTAGA TGCAATTCCT GTCTTCTTGG AAAAGCTGCA AGTTATTCAG 
TGCATTGATG TGGCAGAGCA GGCACTGACA GCCCTAGAGA TGCTATCACG CAGGCATAGT 
AAAGCCATTC TGCAGGCAGG TGGGTTGGCA GACTGCTTGC TGTATCTGGA ATTCTTCAGT 
ATAAATGCGC AGAGGAATGC ACTAGCTATT GCTGCCAATT GCTGCCAGAG TATAACACCT 
GATGAGTTTC ACTTTGTGGC AGACTCTTTG CCACTGCTTA CACAAAGGTT AACCCATCAG 
GACAAGAAGT CTGTTGAAAG CACTTGTCTC TGTTTTGCAC GGCTGGTAGA CAATTTCCAG 
CATGAGGAGA ACTTGCTGCA GCAGGTTGCT TCCAAGGACT TGTTAACAAA CATCCAGCAA 
CTCTTGGTAG TGACACCTCC TATCCTGAGC TCAGGAATGT TCATCATGGT GGTGCGCATG 
TTTTCCTTAA TGTGTTCCAA TTGTCCAACG CTTGCAGTCC AACTTATGAA GCAAAACATT 
GCAGAAACAC TTCACTTTCT CCTTTGTGGA GCCTCAAATG GGAGTTGTCA AGAACAAATT 
GACCTTGTTC CACGAAGTCC TCAAGAACTC TATGAGCTTA CTTCTCTTAT CTGTGAACTG 
ATGCCTTGTC TGCCAAAAGA GGGGATATTT GCTGTTGATA CTATGCTAAA GAAAGGAAAT 
GCACAAAATA CAGATGGTGC GATATGGCAA TGGCGAGATG ACAGGGGTCT CTGGCATCCC 
TATAACAGGA TTGATAGTCG AATAATAGAG GCAGCTCATC AGGTTGGTGA GGATGAGATA 
AGCCTGTCGA CACTTGGACG TGTCTATACT ATTGATTTTA ATTCTATGCA GCAAATAAAT 
GAGGATACTG GAACAGCACG TGCCATTCAG CGGAAACCAA ACCCTTTAGC CAATACAAAC 
ACTAGTGGAC ATTCAGAATT GAAGAAGGAT GATGCTCGAG CACAGCTAAT GAAAGAAGAC 
CCAGAATTGG CAAAATCCTT TATCAAAACA CTGTTTGGTG TTCTTTATGA AGTGTATAGT 
TCTTCAGCTG GACCTGCTGT TAGACACAAG TGCCTTAGAG CAATACTTAG AATAATTTAT 
TTTGCTGATG CTGAACTCCT GAAGGATGTG CTGAAAAACC ATGCTGTGTC AAGTCACATT 
GCCTCCATGC TGTCAAGTCA AGACCTTAAG ATAGTAGTTG GAGCCCTGCA GATGGCAGAA 
ATCTTAATGC AGAAGCTACC TGACATTTTT AGTGTTTACT TCAGAAGAGA AGGAGTGATG 
CATCAAGTGA AAAACTTAGC GGAGTCTGAG GCTTTGCTAA CGAGCCCACC AAAAGTATGC 
ACTAATGGAT CAGGAACACT GGGTACCACT ACAACTATAA GTACTGGAAC AGCCACTGCT 
GCCACTAATG CAGCTGCAGA TTTGGGCTCT CCCAGCTTAC AACACAGCCG GGAGGATTCT 
TTAGATCTGA GCCCACAGGG ACGACTGAGT GATGTTCTGA AGAGAAAAAG ACTGCCAAAA 
CGAGGGCCAA GGAGACCAAA ATATTCTCCC CCAAGAGATG ATGACAAAGT AGACAATCAA 
GCAAAAAGCC CCACGACTAC TCAGTCTCCT AAATCTTCTT TCTTAGCAAG TTTAAATCCC 
AAAACATGGG GAAGATTAAG TACACAGTCT AACAGTAATA ACATTGAACC AGCAAGAACA 
GCAGGTGTAA GTGGTCTTGC AAGAGCTGCT TCTAAGGATA CAATTTCCAA TAACAGAGAA 
AAAATTAAGG GTTGGATTAA GGAGCAAGCC CATAAATTTG TAGACCGTTA TTTTAGTTCG 
GAAAACATGG ATGGAAGCAA TCCTGCACTA AATGTATTAC AGAGACTTTG CACTGCAACT 
GAACAACTTA ACCTCCAGGT GGATGGTGGA ACAGAGTGCC TTGTAGAAAT CCGTAGCATT 
GTCTCGGAGT CTGACGTCTC CTCATTTGAA ATCCAGCATA GTGGATTTGT GAAACAACTG 
CTGCTTTATT TGACATCTAA AAGTGAGAAA GATGCTGTAA GCAGGGATAT CAGATTGAAA 
AGATTTCTTC ATGTATTTTT CTCCTCTCCA CTTCCTGGAG AAGAACCCCT TGGAAGATTA 
GAGCCATTAG AAAATGCACC TTTGTTGGCA TTAGTCCATA AAATGAACAA TTGCCTCAGT 
CAGATGGAGC AGTTTCCAGT GAAAGTGCAT GACTTCCCCA GTGGAAATGG AACAGGGAGC 
AGTTTTTCTC TTAACAGAGG ATCCCAAGCT TTAAAATTCT TCAATACGCA TCAGTTAAAA 
TGCCAGCTGC AAAGACATCC AGACTGTGCT AATGTGAAAC AGTGGAAGGG TGGACCTGTG 
AAGATTGATC CTCTGGCTTT GGTTCAAGCC ATTGAAAGAT ATCTTGTTGT TAGAGGTTAT 
GGAAGAGTTA GAGAAGATGA TGAGGATAGT GATGATGATG GGTCAGATGA AGAAATAGAT 
GAATCCTTGG CTGCTCAATT CTTAAATTCA GGGAATGTGA GACATAGATT GCAGTTTTAC 
ATTGGGGATC ACTTGCTACC ATATAATATG ACTGTATATC AAGCAGTTAA GCTGTACAGT 
TTGCAAGCTG AGGAGGAGAG GGAATCGACT GATGATGAAA GCAATCCATT AGGAAGAGCT 
GGGATTTGGA CAAAAACACA TACCATTTGG TACAAACCTG TACGGGAGGA TGAAGATGGC 
AACAAAGACT GTGTTGGTGG TAAAAGAGGA AGAGCTCAAA CTGCTCCCAC AAAAACCTCA 
CCTAGGAATT CTAAAAAGCA TGATGAGTTG TGGCACGATG GTGTATGCCC TTCAGTATTG 
AATCCTTTAG AAGTTTACCT CATATCTACT CCTCCTGAAA ACATAACCTT TGAAGATCCT 
TCATTAGATG TTATTCTTCT TTTGAGAGTT TTACATGCTA TCAGTCGATA CTGGTATTAT 
TTGTATGATA ATGCAATATG CAAGGAGATT ATTCCAACTT CGGAATTTAT CAATAGTAAA 
CTGACAGCAA AAGCAAACAG ACAGCTTCAA GATCCACTGG TAATTATGAC AGGAAACATA 
CCGACTTGGC TGACAGAGCT TGGAAAAACA TGCCCATTTT TCTTTCCATT TGATACCCGG 
CAAATGCTGT TTTATGTAAC TGCTTTTGAT CGTGATCGAG CCATGCAAAG ACTACTGGAT 
ACTAATCCAG AAATCAATCA ATCAGATTCT CAGGATAGCA GAGTGGCACC ACGACTGGAC 
AGGAAAAAAC GCACTGTGAA CAGAGATGAG CTGTTGAAAC AGGCAGAATC TGTGATGCAG 
GATCTAGGCA GTTCAAGAGC CATGTTGGAA ATCCAGTATG AGAATGAAGT TGGCACAGGC 
CTGGGCCCTA CACTAGAGTT CTATGCACTT GTATCTCAGG AACTACAGAG AGCAGACTTG 
GGCCTTTGGA GGGGAGAAGA AGTAACTTTA GCCAATCCAA AAGGAAGCCA AGAAGGTACC 
AAGTATATTC ATAATCTCCA AGGCCTTTTT GCACTTCCTT TTGGTAGAAC AGCCAAGCCT 
GCTCACATTG CAAAAGTTAA AATGAAGTTC CGCTTTCTGG GAAAACTAAT GGCTAAGGCA 
ATCATGGATT TTAGACTGGT GGATCTTCCT CTTGGACTTC CTTTTTATAA ATGGATGTTA 
CGCCAGGAAA CTTCTTTGAC GTCACATGAC TTGTTCAGTA TTGATCCAGT AGTAGCCAAA 
TCAATATATC ACCTTGAAGA TATTGTAAGA CAAAAGAAAA GACTTGAGCA GGACAAAACA 
CAGACCAAAG AAAGTCTACA GTATGCATTG GAAACCCTGA CTATGAATGG CTGCTCAGTG 
GAGGATCTAG GGCTGGATTT CACACTTCCT GGATTTCCTA ACATCGAGCT GAAAAAAGGG 
GGAAAAGATA CACCAGTCAC CATCCACAAC TTGGAGGAGT ACCTCAGATT GGTTATATTC 
TGGGCACTTA ACGAAGGTGT TGCCAGACAG TTTGATTCTT TCAGAGATGG ATTTGAATCA 
GTCTTCCCCC TCAGTCATCT TCAATACTTT TATCCTGAGG AGTTGGAGCA ACTCTTATGT 
GGCAGCAAAA CAGACACATG GGATGCCAAG ACCTTAATGG AATGTTGCAG GCCAGATCAC 
GGTTATACAC ATGACAGTCG AGCAGTGAAG TATCTCTTTG AAATTCTCAG TAGTTTTGAT 
AGTGAGCAGC AGAGACTGTT TCTTCAGTTT GTGACCGGTA GTCCCAGGCT GCCAGTAGGA 
GGCTTTCGAA GTTTGAATCC TCCATTGACA ATTGTACGGA AGACATTTGA GTCTACAGAG 
AATCCAGATG ACTTCTTGCC CTCAGTAATG ACTTGTGTGA ACTATCTCAA ATTGCCGGAC 
TATTCAACTA TTGAGATAAT GCGTGAAAAA CTCTTGATAG CTGCAAGAGA AGGGCAGCAG 
TCATTCCATC TTTCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TRIP12 ( XM_015276967.1 ) cDNA ORF clone, Gallus gallus(chicken) -> XP_015132453.1 Gallus gallus thyroid hormone receptor interactor 12 (TRIP12), transcript variant X5, mRNA.

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CloneID	OGa12502
Clone ID Related Accession (Same CDS sequence)	XM_015276964.1 , XM_015276968.2 , XM_015276967.1 , XM_015276968.1 , XM_004937064.3 , XM_015276964.2 , XM_015276966.2 , XM_004937064.2 , XM_015276966.1 , XM_015276967.2 , XM_015276965.1
Accession Version	XM_015276967.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6198bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-03
Organism	Gallus gallus(chicken)
Product	E3 ubiquitin-protein ligase TRIP12 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_001471743.3) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Gallus gallus Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCCAACC GGCCTAATAA CAATCCAGGG GGGTCACTGC GACGTTCACA GAGGAACACT 
GCTGGGGCCC AGCCACAAGA CGACGCAGCA GGAGGAAGGT CACATTTAGG GCAGGCAAAA 
CATAAGGCAC ATAGCCCTCC TGAGAGTAGA AAATCTATTT CAAAGACACC CAAAGTGCAG 
TCTAATACTA CTTCTGAGCA GTCCAAGGGA CACTTTTCTA AAAGAGGCTG TAGTTCATCT 
GCTGTTTTAA TACCACAAGA AGATTCAGAA AGAGTCAGTA CTTCAGAAAA GCAAAAAACG 
GGGCAAGTGC CTAAGAAAGA CAATTCTCGA GGAGTTAAAC GCAGTGCTAG TCCAGATTAC 
AGCAGGACCA ATTCTCCTAG CTCTGCTAAA AAACCTAAAG CACTTCAACA CGCCGAAACT 
TCCTCGGAAA GTAACAAGCC ACATACTAAA TCCAAGAGAA GACACTTAGA CCAAGAACAG 
CAGAAGTCTA CACAATTGCC ATCAACAAGC AAGGCTCACA CCAGAAAGGG TGGAGCTGCT 
GGTAGCTCCC GAAGTCAGAA AAGGAAAAGG ACAGAGAGTA CGTCTTGTAT AAAGAGTCGT 
TCAGCAGTTG AATCAACTGG AGCTGAAGAG AAGTCAGCAA AATCCTCCAA GCTGGCTTCA 
AAATCGGTGA CCTCAGCCAA AGCTGGGTGT AGCACCATCA CTGATTCTTC TTCTTCAGCT 
TCTACATCCT CTTCCTCTTC TGCTGTTGCC TCTGCTTCTT CCACTGTTCC TCAGGGTGCC 
AGAGTGAAAC AGGGTAAGGA CCAGAATAAG GCTAGACGTT CACGTTCTGC GTCCAGCCCT 
AGTCCAAGAA GGAGCAGCAG GGACAAAGAA CAGAGCAAAA CAGGTGGCTC TTCAAAGTTT 
GACTGGGCTG CTCGATTCAG TCCAAAAGTT AGTCTCCCTA AAACAAAACT GTCTCTACCA 
GGCTCTTCTA AGTCAGAGAC CTCAAAACCT GGACCTTCAG GACTACAGGC TAAGCTAGCA 
AGCCTAAGAA AATCCACAAA GAAGCGCAGT GAATCACCAC CTGCTGAGCT CCCCAGTTTA 
CGGCGGAGCA CACGGCAAAA GACCACGGGC TCCTGTGCGA GTACCAGTCG GCGGGGCTCT 
GGCCTGGGCA AAAGAGGAGC AGCTGAAGCT CGCCGACAGG AGAAGATGGC TGATCCTGAC 
AACAACCAGG ATGGAGTTAA CTCTTCAGCT GCACGTACAG ATGAGACTCC CCAGGGAGCT 
GCAGCTTCTA GCTCTGTTGC TGGGGCTGTA GGTATGACAA CATCTGGAGA AAGTGAGTCA 
GATGACTCTG AGATGGGAAG ACTACAAGCT CTGTTAGAGG CTAGGGGTCT TCCTCCTCAC 
CTGTTTGGCC CTCTTGGTCC TCGGATGTCG CAGCTCTTCC ACAGGACAAT TGGAAGTGGA 
GCTAGTTCTA AAGCCCAGCA GCTTTTACAA GGTCTCCAAG CCACTGATGA AAGTCAGCAA 
CTACAGGCAG TTATTGAGAT GTGCCAGCTG TTGGTCATGG GAAATGAAGA AACACTGGGA 
GGATTTCCAG TCAAGAGTGT TGTGCCAGCT TTGATAACAT TGCTGCAGAT GGAGCATAAC 
TTTGACATCA TGAACCATGC TTGTCGAGCC TTAACATACA TGATGGAAGC ACTTCCCAGA 
TCATCTGCTG TAGTGGTAGA TGCAATTCCT GTCTTCTTGG AAAAGCTGCA AGTTATTCAG 
TGCATTGATG TGGCAGAGCA GGCACTGACA GCCCTAGAGA TGCTATCACG CAGGCATAGT 
AAAGCCATTC TGCAGGCAGG TGGGTTGGCA GACTGCTTGC TGTATCTGGA ATTCTTCAGT 
ATAAATGCGC AGAGGAATGC ACTAGCTATT GCTGCCAATT GCTGCCAGAG TATAACACCT 
GATGAGTTTC ACTTTGTGGC AGACTCTTTG CCACTGCTTA CACAAAGGTT AACCCATCAG 
GACAAGAAGT CTGTTGAAAG CACTTGTCTC TGTTTTGCAC GGCTGGTAGA CAATTTCCAG 
CATGAGGAGA ACTTGCTGCA GCAGGTTGCT TCCAAGGACT TGTTAACAAA CATCCAGCAA 
CTCTTGGTAG TGACACCTCC TATCCTGAGC TCAGGAATGT TCATCATGGT GGTGCGCATG 
TTTTCCTTAA TGTGTTCCAA TTGTCCAACG CTTGCAGTCC AACTTATGAA GCAAAACATT 
GCAGAAACAC TTCACTTTCT CCTTTGTGGA GCCTCAAATG GGAGTTGTCA AGAACAAATT 
GACCTTGTTC CACGAAGTCC TCAAGAACTC TATGAGCTTA CTTCTCTTAT CTGTGAACTG 
ATGCCTTGTC TGCCAAAAGA GGGGATATTT GCTGTTGATA CTATGCTAAA GAAAGGAAAT 
GCACAAAATA CAGATGGTGC GATATGGCAA TGGCGAGATG ACAGGGGTCT CTGGCATCCC 
TATAACAGGA TTGATAGTCG AATAATAGAG GCAGCTCATC AGGTTGGTGA GGATGAGATA 
AGCCTGTCGA CACTTGGACG TGTCTATACT ATTGATTTTA ATTCTATGCA GCAAATAAAT 
GAGGATACTG GAACAGCACG TGCCATTCAG CGGAAACCAA ACCCTTTAGC CAATACAAAC 
ACTAGTGGAC ATTCAGAATT GAAGAAGGAT GATGCTCGAG CACAGCTAAT GAAAGAAGAC 
CCAGAATTGG CAAAATCCTT TATCAAAACA CTGTTTGGTG TTCTTTATGA AGTGTATAGT 
TCTTCAGCTG GACCTGCTGT TAGACACAAG TGCCTTAGAG CAATACTTAG AATAATTTAT 
TTTGCTGATG CTGAACTCCT GAAGGATGTG CTGAAAAACC ATGCTGTGTC AAGTCACATT 
GCCTCCATGC TGTCAAGTCA AGACCTTAAG ATAGTAGTTG GAGCCCTGCA GATGGCAGAA 
ATCTTAATGC AGAAGCTACC TGACATTTTT AGTGTTTACT TCAGAAGAGA AGGAGTGATG 
CATCAAGTGA AAAACTTAGC GGAGTCTGAG GCTTTGCTAA CGAGCCCACC AAAAGTATGC 
ACTAATGGAT CAGGAACACT GGGTACCACT ACAACTATAA GTACTGGAAC AGCCACTGCT 
GCCACTAATG CAGCTGCAGA TTTGGGCTCT CCCAGCTTAC AACACAGCCG GGAGGATTCT 
TTAGATCTGA GCCCACAGGG ACGACTGAGT GATGTTCTGA AGAGAAAAAG ACTGCCAAAA 
CGAGGGCCAA GGAGACCAAA ATATTCTCCC CCAAGAGATG ATGACAAAGT AGACAATCAA 
GCAAAAAGCC CCACGACTAC TCAGTCTCCT AAATCTTCTT TCTTAGCAAG TTTAAATCCC 
AAAACATGGG GAAGATTAAG TACACAGTCT AACAGTAATA ACATTGAACC AGCAAGAACA 
GCAGGTGTAA GTGGTCTTGC AAGAGCTGCT TCTAAGGATA CAATTTCCAA TAACAGAGAA 
AAAATTAAGG GTTGGATTAA GGAGCAAGCC CATAAATTTG TAGACCGTTA TTTTAGTTCG 
GAAAACATGG ATGGAAGCAA TCCTGCACTA AATGTATTAC AGAGACTTTG CACTGCAACT 
GAACAACTTA ACCTCCAGGT GGATGGTGGA ACAGAGTGCC TTGTAGAAAT CCGTAGCATT 
GTCTCGGAGT CTGACGTCTC CTCATTTGAA ATCCAGCATA GTGGATTTGT GAAACAACTG 
CTGCTTTATT TGACATCTAA AAGTGAGAAA GATGCTGTAA GCAGGGATAT CAGATTGAAA 
AGATTTCTTC ATGTATTTTT CTCCTCTCCA CTTCCTGGAG AAGAACCCCT TGGAAGATTA 
GAGCCATTAG AAAATGCACC TTTGTTGGCA TTAGTCCATA AAATGAACAA TTGCCTCAGT 
CAGATGGAGC AGTTTCCAGT GAAAGTGCAT GACTTCCCCA GTGGAAATGG AACAGGGAGC 
AGTTTTTCTC TTAACAGAGG ATCCCAAGCT TTAAAATTCT TCAATACGCA TCAGTTAAAA 
TGCCAGCTGC AAAGACATCC AGACTGTGCT AATGTGAAAC AGTGGAAGGG TGGACCTGTG 
AAGATTGATC CTCTGGCTTT GGTTCAAGCC ATTGAAAGAT ATCTTGTTGT TAGAGGTTAT 
GGAAGAGTTA GAGAAGATGA TGAGGATAGT GATGATGATG GGTCAGATGA AGAAATAGAT 
GAATCCTTGG CTGCTCAATT CTTAAATTCA GGGAATGTGA GACATAGATT GCAGTTTTAC 
ATTGGGGATC ACTTGCTACC ATATAATATG ACTGTATATC AAGCAGTTAA GCTGTACAGT 
TTGCAAGCTG AGGAGGAGAG GGAATCGACT GATGATGAAA GCAATCCATT AGGAAGAGCT 
GGGATTTGGA CAAAAACACA TACCATTTGG TACAAACCTG TACGGGAGGA TGAAGATGGC 
AACAAAGACT GTGTTGGTGG TAAAAGAGGA AGAGCTCAAA CTGCTCCCAC AAAAACCTCA 
CCTAGGAATT CTAAAAAGCA TGATGAGTTG TGGCACGATG GTGTATGCCC TTCAGTATTG 
AATCCTTTAG AAGTTTACCT CATATCTACT CCTCCTGAAA ACATAACCTT TGAAGATCCT 
TCATTAGATG TTATTCTTCT TTTGAGAGTT TTACATGCTA TCAGTCGATA CTGGTATTAT 
TTGTATGATA ATGCAATATG CAAGGAGATT ATTCCAACTT CGGAATTTAT CAATAGTAAA 
CTGACAGCAA AAGCAAACAG ACAGCTTCAA GATCCACTGG TAATTATGAC AGGAAACATA 
CCGACTTGGC TGACAGAGCT TGGAAAAACA TGCCCATTTT TCTTTCCATT TGATACCCGG 
CAAATGCTGT TTTATGTAAC TGCTTTTGAT CGTGATCGAG CCATGCAAAG ACTACTGGAT 
ACTAATCCAG AAATCAATCA ATCAGATTCT CAGGATAGCA GAGTGGCACC ACGACTGGAC 
AGGAAAAAAC GCACTGTGAA CAGAGATGAG CTGTTGAAAC AGGCAGAATC TGTGATGCAG 
GATCTAGGCA GTTCAAGAGC CATGTTGGAA ATCCAGTATG AGAATGAAGT TGGCACAGGC 
CTGGGCCCTA CACTAGAGTT CTATGCACTT GTATCTCAGG AACTACAGAG AGCAGACTTG 
GGCCTTTGGA GGGGAGAAGA AGTAACTTTA GCCAATCCAA AAGGAAGCCA AGAAGGTACC 
AAGTATATTC ATAATCTCCA AGGCCTTTTT GCACTTCCTT TTGGTAGAAC AGCCAAGCCT 
GCTCACATTG CAAAAGTTAA AATGAAGTTC CGCTTTCTGG GAAAACTAAT GGCTAAGGCA 
ATCATGGATT TTAGACTGGT GGATCTTCCT CTTGGACTTC CTTTTTATAA ATGGATGTTA 
CGCCAGGAAA CTTCTTTGAC GTCACATGAC TTGTTCAGTA TTGATCCAGT AGTAGCCAAA 
TCAATATATC ACCTTGAAGA TATTGTAAGA CAAAAGAAAA GACTTGAGCA GGACAAAACA 
CAGACCAAAG AAAGTCTACA GTATGCATTG GAAACCCTGA CTATGAATGG CTGCTCAGTG 
GAGGATCTAG GGCTGGATTT CACACTTCCT GGATTTCCTA ACATCGAGCT GAAAAAAGGG 
GGAAAAGATA CACCAGTCAC CATCCACAAC TTGGAGGAGT ACCTCAGATT GGTTATATTC 
TGGGCACTTA ACGAAGGTGT TGCCAGACAG TTTGATTCTT TCAGAGATGG ATTTGAATCA 
GTCTTCCCCC TCAGTCATCT TCAATACTTT TATCCTGAGG AGTTGGAGCA ACTCTTATGT 
GGCAGCAAAA CAGACACATG GGATGCCAAG ACCTTAATGG AATGTTGCAG GCCAGATCAC 
GGTTATACAC ATGACAGTCG AGCAGTGAAG TATCTCTTTG AAATTCTCAG TAGTTTTGAT 
AGTGAGCAGC AGAGACTGTT TCTTCAGTTT GTGACCGGTA GTCCCAGGCT GCCAGTAGGA 
GGCTTTCGAA GTTTGAATCC TCCATTGACA ATTGTACGGA AGACATTTGA GTCTACAGAG 
AATCCAGATG ACTTCTTGCC CTCAGTAATG ACTTGTGTGA ACTATCTCAA ATTGCCGGAC 
TATTCAACTA TTGAGATAAT GCGTGAAAAA CTCTTGATAG CTGCAAGAGA AGGGCAGCAG 
TCATTCCATC TTTCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015132453.1
CDS	74..6271
Translation	MSNRPNNNPGGSLRRSQRNTAGAQPQDDAAGGRSHLGQAKHKAHSPPESRKSISKTPKVQSNTTSEQSKGHFSKRGCSSSAVLIPQEDSERVSTSEKQKTGQVPKKDNSRGVKRSASPDYSRTNSPSSAKKPKALQHAETSSESNKPHTKSKRRHLDQEQQKSTQLPSTSKAHTRKGGAAGSSRSQKRKRTESTSCIKSRSAVESTGAEEKSAKSSKLASKSVTSAKAGCSTITDSSSSASTSSSSSAVASASSTVPQGARVKQGKDQNKARRSRSASSPSPRRSSRDKEQSKTGGSSKFDWAARFSPKVSLPKTKLSLPGSSKSETSKPGPSGLQAKLASLRKSTKKRSESPPAELPSLRRSTRQKTTGSCASTSRRGSGLGKRGAAEARRQEKMADPDNNQDGVNSSAARTDETPQGAAASSSVAGAVGMTTSGESESDDSEMGRLQALLEARGLPPHLFGPLGPRMSQLFHRTIGSGASSKAQQLLQGLQATDESQQLQAVIEMCQLLVMGNEETLGGFPVKSVVPALITLLQMEHNFDIMNHACRALTYMMEALPRSSAVVVDAIPVFLEKLQVIQCIDVAEQALTALEMLSRRHSKAILQAGGLADCLLYLEFFSINAQRNALAIAANCCQSITPDEFHFVADSLPLLTQRLTHQDKKSVESTCLCFARLVDNFQHEENLLQQVASKDLLTNIQQLLVVTPPILSSGMFIMVVRMFSLMCSNCPTLAVQLMKQNIAETLHFLLCGASNGSCQEQIDLVPRSPQELYELTSLICELMPCLPKEGIFAVDTMLKKGNAQNTDGAIWQWRDDRGLWHPYNRIDSRIIEAAHQVGEDEISLSTLGRVYTIDFNSMQQINEDTGTARAIQRKPNPLANTNTSGHSELKKDDARAQLMKEDPELAKSFIKTLFGVLYEVYSSSAGPAVRHKCLRAILRIIYFADAELLKDVLKNHAVSSHIASMLSSQDLKIVVGALQMAEILMQKLPDIFSVYFRREGVMHQVKNLAESEALLTSPPKVCTNGSGTLGTTTTISTGTATAATNAAADLGSPSLQHSREDSLDLSPQGRLSDVLKRKRLPKRGPRRPKYSPPRDDDKVDNQAKSPTTTQSPKSSFLASLNPKTWGRLSTQSNSNNIEPARTAGVSGLARAASKDTISNNREKIKGWIKEQAHKFVDRYFSSENMDGSNPALNVLQRLCTATEQLNLQVDGGTECLVEIRSIVSESDVSSFEIQHSGFVKQLLLYLTSKSEKDAVSRDIRLKRFLHVFFSSPLPGEEPLGRLEPLENAPLLALVHKMNNCLSQMEQFPVKVHDFPSGNGTGSSFSLNRGSQALKFFNTHQLKCQLQRHPDCANVKQWKGGPVKIDPLALVQAIERYLVVRGYGRVREDDEDSDDDGSDEEIDESLAAQFLNSGNVRHRLQFYIGDHLLPYNMTVYQAVKLYSLQAEEERESTDDESNPLGRAGIWTKTHTIWYKPVREDEDGNKDCVGGKRGRAQTAPTKTSPRNSKKHDELWHDGVCPSVLNPLEVYLISTPPENITFEDPSLDVILLLRVLHAISRYWYYLYDNAICKEIIPTSEFINSKLTAKANRQLQDPLVIMTGNIPTWLTELGKTCPFFFPFDTRQMLFYVTAFDRDRAMQRLLDTNPEINQSDSQDSRVAPRLDRKKRTVNRDELLKQAESVMQDLGSSRAMLEIQYENEVGTGLGPTLEFYALVSQELQRADLGLWRGEEVTLANPKGSQEGTKYIHNLQGLFALPFGRTAKPAHIAKVKMKFRFLGKLMAKAIMDFRLVDLPLGLPFYKWMLRQETSLTSHDLFSIDPVVAKSIYHLEDIVRQKKRLEQDKTQTKESLQYALETLTMNGCSVEDLGLDFTLPGFPNIELKKGGKDTPVTIHNLEEYLRLVIFWALNEGVARQFDSFRDGFESVFPLSHLQYFYPEELEQLLCGSKTDTWDAKTLMECCRPDHGYTHDSRAVKYLFEILSSFDSEQQRLFLQFVTGSPRLPVGGFRSLNPPLTIVRKTFESTENPDDFLPSVMTCVNYLKLPDYSTIEIMREKLLIAAREGQQSFHLS

Target ORF information:

RefSeq Version	XM_015276967.1
Organism	Gallus gallus(chicken)
Definition	Gallus gallus thyroid hormone receptor interactor 12 (TRIP12), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015276967.1

ORF Insert Sequence:

ATGTCCAACC GGCCTAATAA CAATCCAGGG GGGTCACTGC GACGTTCACA GAGGAACACT 
GCTGGGGCCC AGCCACAAGA CGACGCAGCA GGAGGAAGGT CACATTTAGG GCAGGCAAAA 
CATAAGGCAC ATAGCCCTCC TGAGAGTAGA AAATCTATTT CAAAGACACC CAAAGTGCAG 
TCTAATACTA CTTCTGAGCA GTCCAAGGGA CACTTTTCTA AAAGAGGCTG TAGTTCATCT 
GCTGTTTTAA TACCACAAGA AGATTCAGAA AGAGTCAGTA CTTCAGAAAA GCAAAAAACG 
GGGCAAGTGC CTAAGAAAGA CAATTCTCGA GGAGTTAAAC GCAGTGCTAG TCCAGATTAC 
AGCAGGACCA ATTCTCCTAG CTCTGCTAAA AAACCTAAAG CACTTCAACA CGCCGAAACT 
TCCTCGGAAA GTAACAAGCC ACATACTAAA TCCAAGAGAA GACACTTAGA CCAAGAACAG 
CAGAAGTCTA CACAATTGCC ATCAACAAGC AAGGCTCACA CCAGAAAGGG TGGAGCTGCT 
GGTAGCTCCC GAAGTCAGAA AAGGAAAAGG ACAGAGAGTA CGTCTTGTAT AAAGAGTCGT 
TCAGCAGTTG AATCAACTGG AGCTGAAGAG AAGTCAGCAA AATCCTCCAA GCTGGCTTCA 
AAATCGGTGA CCTCAGCCAA AGCTGGGTGT AGCACCATCA CTGATTCTTC TTCTTCAGCT 
TCTACATCCT CTTCCTCTTC TGCTGTTGCC TCTGCTTCTT CCACTGTTCC TCAGGGTGCC 
AGAGTGAAAC AGGGTAAGGA CCAGAATAAG GCTAGACGTT CACGTTCTGC GTCCAGCCCT 
AGTCCAAGAA GGAGCAGCAG GGACAAAGAA CAGAGCAAAA CAGGTGGCTC TTCAAAGTTT 
GACTGGGCTG CTCGATTCAG TCCAAAAGTT AGTCTCCCTA AAACAAAACT GTCTCTACCA 
GGCTCTTCTA AGTCAGAGAC CTCAAAACCT GGACCTTCAG GACTACAGGC TAAGCTAGCA 
AGCCTAAGAA AATCCACAAA GAAGCGCAGT GAATCACCAC CTGCTGAGCT CCCCAGTTTA 
CGGCGGAGCA CACGGCAAAA GACCACGGGC TCCTGTGCGA GTACCAGTCG GCGGGGCTCT 
GGCCTGGGCA AAAGAGGAGC AGCTGAAGCT CGCCGACAGG AGAAGATGGC TGATCCTGAC 
AACAACCAGG ATGGAGTTAA CTCTTCAGCT GCACGTACAG ATGAGACTCC CCAGGGAGCT 
GCAGCTTCTA GCTCTGTTGC TGGGGCTGTA GGTATGACAA CATCTGGAGA AAGTGAGTCA 
GATGACTCTG AGATGGGAAG ACTACAAGCT CTGTTAGAGG CTAGGGGTCT TCCTCCTCAC 
CTGTTTGGCC CTCTTGGTCC TCGGATGTCG CAGCTCTTCC ACAGGACAAT TGGAAGTGGA 
GCTAGTTCTA AAGCCCAGCA GCTTTTACAA GGTCTCCAAG CCACTGATGA AAGTCAGCAA 
CTACAGGCAG TTATTGAGAT GTGCCAGCTG TTGGTCATGG GAAATGAAGA AACACTGGGA 
GGATTTCCAG TCAAGAGTGT TGTGCCAGCT TTGATAACAT TGCTGCAGAT GGAGCATAAC 
TTTGACATCA TGAACCATGC TTGTCGAGCC TTAACATACA TGATGGAAGC ACTTCCCAGA 
TCATCTGCTG TAGTGGTAGA TGCAATTCCT GTCTTCTTGG AAAAGCTGCA AGTTATTCAG 
TGCATTGATG TGGCAGAGCA GGCACTGACA GCCCTAGAGA TGCTATCACG CAGGCATAGT 
AAAGCCATTC TGCAGGCAGG TGGGTTGGCA GACTGCTTGC TGTATCTGGA ATTCTTCAGT 
ATAAATGCGC AGAGGAATGC ACTAGCTATT GCTGCCAATT GCTGCCAGAG TATAACACCT 
GATGAGTTTC ACTTTGTGGC AGACTCTTTG CCACTGCTTA CACAAAGGTT AACCCATCAG 
GACAAGAAGT CTGTTGAAAG CACTTGTCTC TGTTTTGCAC GGCTGGTAGA CAATTTCCAG 
CATGAGGAGA ACTTGCTGCA GCAGGTTGCT TCCAAGGACT TGTTAACAAA CATCCAGCAA 
CTCTTGGTAG TGACACCTCC TATCCTGAGC TCAGGAATGT TCATCATGGT GGTGCGCATG 
TTTTCCTTAA TGTGTTCCAA TTGTCCAACG CTTGCAGTCC AACTTATGAA GCAAAACATT 
GCAGAAACAC TTCACTTTCT CCTTTGTGGA GCCTCAAATG GGAGTTGTCA AGAACAAATT 
GACCTTGTTC CACGAAGTCC TCAAGAACTC TATGAGCTTA CTTCTCTTAT CTGTGAACTG 
ATGCCTTGTC TGCCAAAAGA GGGGATATTT GCTGTTGATA CTATGCTAAA GAAAGGAAAT 
GCACAAAATA CAGATGGTGC GATATGGCAA TGGCGAGATG ACAGGGGTCT CTGGCATCCC 
TATAACAGGA TTGATAGTCG AATAATAGAG GCAGCTCATC AGGTTGGTGA GGATGAGATA 
AGCCTGTCGA CACTTGGACG TGTCTATACT ATTGATTTTA ATTCTATGCA GCAAATAAAT 
GAGGATACTG GAACAGCACG TGCCATTCAG CGGAAACCAA ACCCTTTAGC CAATACAAAC 
ACTAGTGGAC ATTCAGAATT GAAGAAGGAT GATGCTCGAG CACAGCTAAT GAAAGAAGAC 
CCAGAATTGG CAAAATCCTT TATCAAAACA CTGTTTGGTG TTCTTTATGA AGTGTATAGT 
TCTTCAGCTG GACCTGCTGT TAGACACAAG TGCCTTAGAG CAATACTTAG AATAATTTAT 
TTTGCTGATG CTGAACTCCT GAAGGATGTG CTGAAAAACC ATGCTGTGTC AAGTCACATT 
GCCTCCATGC TGTCAAGTCA AGACCTTAAG ATAGTAGTTG GAGCCCTGCA GATGGCAGAA 
ATCTTAATGC AGAAGCTACC TGACATTTTT AGTGTTTACT TCAGAAGAGA AGGAGTGATG 
CATCAAGTGA AAAACTTAGC GGAGTCTGAG GCTTTGCTAA CGAGCCCACC AAAAGTATGC 
ACTAATGGAT CAGGAACACT GGGTACCACT ACAACTATAA GTACTGGAAC AGCCACTGCT 
GCCACTAATG CAGCTGCAGA TTTGGGCTCT CCCAGCTTAC AACACAGCCG GGAGGATTCT 
TTAGATCTGA GCCCACAGGG ACGACTGAGT GATGTTCTGA AGAGAAAAAG ACTGCCAAAA 
CGAGGGCCAA GGAGACCAAA ATATTCTCCC CCAAGAGATG ATGACAAAGT AGACAATCAA 
GCAAAAAGCC CCACGACTAC TCAGTCTCCT AAATCTTCTT TCTTAGCAAG TTTAAATCCC 
AAAACATGGG GAAGATTAAG TACACAGTCT AACAGTAATA ACATTGAACC AGCAAGAACA 
GCAGGTGTAA GTGGTCTTGC AAGAGCTGCT TCTAAGGATA CAATTTCCAA TAACAGAGAA 
AAAATTAAGG GTTGGATTAA GGAGCAAGCC CATAAATTTG TAGACCGTTA TTTTAGTTCG 
GAAAACATGG ATGGAAGCAA TCCTGCACTA AATGTATTAC AGAGACTTTG CACTGCAACT 
GAACAACTTA ACCTCCAGGT GGATGGTGGA ACAGAGTGCC TTGTAGAAAT CCGTAGCATT 
GTCTCGGAGT CTGACGTCTC CTCATTTGAA ATCCAGCATA GTGGATTTGT GAAACAACTG 
CTGCTTTATT TGACATCTAA AAGTGAGAAA GATGCTGTAA GCAGGGATAT CAGATTGAAA 
AGATTTCTTC ATGTATTTTT CTCCTCTCCA CTTCCTGGAG AAGAACCCCT TGGAAGATTA 
GAGCCATTAG AAAATGCACC TTTGTTGGCA TTAGTCCATA AAATGAACAA TTGCCTCAGT 
CAGATGGAGC AGTTTCCAGT GAAAGTGCAT GACTTCCCCA GTGGAAATGG AACAGGGAGC 
AGTTTTTCTC TTAACAGAGG ATCCCAAGCT TTAAAATTCT TCAATACGCA TCAGTTAAAA 
TGCCAGCTGC AAAGACATCC AGACTGTGCT AATGTGAAAC AGTGGAAGGG TGGACCTGTG 
AAGATTGATC CTCTGGCTTT GGTTCAAGCC ATTGAAAGAT ATCTTGTTGT TAGAGGTTAT 
GGAAGAGTTA GAGAAGATGA TGAGGATAGT GATGATGATG GGTCAGATGA AGAAATAGAT 
GAATCCTTGG CTGCTCAATT CTTAAATTCA GGGAATGTGA GACATAGATT GCAGTTTTAC 
ATTGGGGATC ACTTGCTACC ATATAATATG ACTGTATATC AAGCAGTTAA GCTGTACAGT 
TTGCAAGCTG AGGAGGAGAG GGAATCGACT GATGATGAAA GCAATCCATT AGGAAGAGCT 
GGGATTTGGA CAAAAACACA TACCATTTGG TACAAACCTG TACGGGAGGA TGAAGATGGC 
AACAAAGACT GTGTTGGTGG TAAAAGAGGA AGAGCTCAAA CTGCTCCCAC AAAAACCTCA 
CCTAGGAATT CTAAAAAGCA TGATGAGTTG TGGCACGATG GTGTATGCCC TTCAGTATTG 
AATCCTTTAG AAGTTTACCT CATATCTACT CCTCCTGAAA ACATAACCTT TGAAGATCCT 
TCATTAGATG TTATTCTTCT TTTGAGAGTT TTACATGCTA TCAGTCGATA CTGGTATTAT 
TTGTATGATA ATGCAATATG CAAGGAGATT ATTCCAACTT CGGAATTTAT CAATAGTAAA 
CTGACAGCAA AAGCAAACAG ACAGCTTCAA GATCCACTGG TAATTATGAC AGGAAACATA 
CCGACTTGGC TGACAGAGCT TGGAAAAACA TGCCCATTTT TCTTTCCATT TGATACCCGG 
CAAATGCTGT TTTATGTAAC TGCTTTTGAT CGTGATCGAG CCATGCAAAG ACTACTGGAT 
ACTAATCCAG AAATCAATCA ATCAGATTCT CAGGATAGCA GAGTGGCACC ACGACTGGAC 
AGGAAAAAAC GCACTGTGAA CAGAGATGAG CTGTTGAAAC AGGCAGAATC TGTGATGCAG 
GATCTAGGCA GTTCAAGAGC CATGTTGGAA ATCCAGTATG AGAATGAAGT TGGCACAGGC 
CTGGGCCCTA CACTAGAGTT CTATGCACTT GTATCTCAGG AACTACAGAG AGCAGACTTG 
GGCCTTTGGA GGGGAGAAGA AGTAACTTTA GCCAATCCAA AAGGAAGCCA AGAAGGTACC 
AAGTATATTC ATAATCTCCA AGGCCTTTTT GCACTTCCTT TTGGTAGAAC AGCCAAGCCT 
GCTCACATTG CAAAAGTTAA AATGAAGTTC CGCTTTCTGG GAAAACTAAT GGCTAAGGCA 
ATCATGGATT TTAGACTGGT GGATCTTCCT CTTGGACTTC CTTTTTATAA ATGGATGTTA 
CGCCAGGAAA CTTCTTTGAC GTCACATGAC TTGTTCAGTA TTGATCCAGT AGTAGCCAAA 
TCAATATATC ACCTTGAAGA TATTGTAAGA CAAAAGAAAA GACTTGAGCA GGACAAAACA 
CAGACCAAAG AAAGTCTACA GTATGCATTG GAAACCCTGA CTATGAATGG CTGCTCAGTG 
GAGGATCTAG GGCTGGATTT CACACTTCCT GGATTTCCTA ACATCGAGCT GAAAAAAGGG 
GGAAAAGATA CACCAGTCAC CATCCACAAC TTGGAGGAGT ACCTCAGATT GGTTATATTC 
TGGGCACTTA ACGAAGGTGT TGCCAGACAG TTTGATTCTT TCAGAGATGG ATTTGAATCA 
GTCTTCCCCC TCAGTCATCT TCAATACTTT TATCCTGAGG AGTTGGAGCA ACTCTTATGT 
GGCAGCAAAA CAGACACATG GGATGCCAAG ACCTTAATGG AATGTTGCAG GCCAGATCAC 
GGTTATACAC ATGACAGTCG AGCAGTGAAG TATCTCTTTG AAATTCTCAG TAGTTTTGAT 
AGTGAGCAGC AGAGACTGTT TCTTCAGTTT GTGACCGGTA GTCCCAGGCT GCCAGTAGGA 
GGCTTTCGAA GTTTGAATCC TCCATTGACA ATTGTACGGA AGACATTTGA GTCTACAGAG 
AATCCAGATG ACTTCTTGCC CTCAGTAATG ACTTGTGTGA ACTATCTCAA ATTGCCGGAC 
TATTCAACTA TTGAGATAAT GCGTGAAAAA CTCTTGATAG CTGCAAGAGA AGGGCAGCAG 
TCATTCCATC TTTCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TRIP12 ( XM_015276968.1 ) cDNA ORF clone, Gallus gallus(chicken) -> XP_015132454.1 Gallus gallus thyroid hormone receptor interactor 12 (TRIP12), transcript variant X6, mRNA.

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CloneID	OGa12502
Clone ID Related Accession (Same CDS sequence)	XM_015276964.1 , XM_015276968.2 , XM_015276967.1 , XM_015276968.1 , XM_004937064.3 , XM_015276964.2 , XM_015276966.2 , XM_004937064.2 , XM_015276966.1 , XM_015276967.2 , XM_015276965.1
Accession Version	XM_015276968.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6198bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-03
Organism	Gallus gallus(chicken)
Product	E3 ubiquitin-protein ligase TRIP12 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_001471743.3) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Gallus gallus Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCCAACC GGCCTAATAA CAATCCAGGG GGGTCACTGC GACGTTCACA GAGGAACACT 
GCTGGGGCCC AGCCACAAGA CGACGCAGCA GGAGGAAGGT CACATTTAGG GCAGGCAAAA 
CATAAGGCAC ATAGCCCTCC TGAGAGTAGA AAATCTATTT CAAAGACACC CAAAGTGCAG 
TCTAATACTA CTTCTGAGCA GTCCAAGGGA CACTTTTCTA AAAGAGGCTG TAGTTCATCT 
GCTGTTTTAA TACCACAAGA AGATTCAGAA AGAGTCAGTA CTTCAGAAAA GCAAAAAACG 
GGGCAAGTGC CTAAGAAAGA CAATTCTCGA GGAGTTAAAC GCAGTGCTAG TCCAGATTAC 
AGCAGGACCA ATTCTCCTAG CTCTGCTAAA AAACCTAAAG CACTTCAACA CGCCGAAACT 
TCCTCGGAAA GTAACAAGCC ACATACTAAA TCCAAGAGAA GACACTTAGA CCAAGAACAG 
CAGAAGTCTA CACAATTGCC ATCAACAAGC AAGGCTCACA CCAGAAAGGG TGGAGCTGCT 
GGTAGCTCCC GAAGTCAGAA AAGGAAAAGG ACAGAGAGTA CGTCTTGTAT AAAGAGTCGT 
TCAGCAGTTG AATCAACTGG AGCTGAAGAG AAGTCAGCAA AATCCTCCAA GCTGGCTTCA 
AAATCGGTGA CCTCAGCCAA AGCTGGGTGT AGCACCATCA CTGATTCTTC TTCTTCAGCT 
TCTACATCCT CTTCCTCTTC TGCTGTTGCC TCTGCTTCTT CCACTGTTCC TCAGGGTGCC 
AGAGTGAAAC AGGGTAAGGA CCAGAATAAG GCTAGACGTT CACGTTCTGC GTCCAGCCCT 
AGTCCAAGAA GGAGCAGCAG GGACAAAGAA CAGAGCAAAA CAGGTGGCTC TTCAAAGTTT 
GACTGGGCTG CTCGATTCAG TCCAAAAGTT AGTCTCCCTA AAACAAAACT GTCTCTACCA 
GGCTCTTCTA AGTCAGAGAC CTCAAAACCT GGACCTTCAG GACTACAGGC TAAGCTAGCA 
AGCCTAAGAA AATCCACAAA GAAGCGCAGT GAATCACCAC CTGCTGAGCT CCCCAGTTTA 
CGGCGGAGCA CACGGCAAAA GACCACGGGC TCCTGTGCGA GTACCAGTCG GCGGGGCTCT 
GGCCTGGGCA AAAGAGGAGC AGCTGAAGCT CGCCGACAGG AGAAGATGGC TGATCCTGAC 
AACAACCAGG ATGGAGTTAA CTCTTCAGCT GCACGTACAG ATGAGACTCC CCAGGGAGCT 
GCAGCTTCTA GCTCTGTTGC TGGGGCTGTA GGTATGACAA CATCTGGAGA AAGTGAGTCA 
GATGACTCTG AGATGGGAAG ACTACAAGCT CTGTTAGAGG CTAGGGGTCT TCCTCCTCAC 
CTGTTTGGCC CTCTTGGTCC TCGGATGTCG CAGCTCTTCC ACAGGACAAT TGGAAGTGGA 
GCTAGTTCTA AAGCCCAGCA GCTTTTACAA GGTCTCCAAG CCACTGATGA AAGTCAGCAA 
CTACAGGCAG TTATTGAGAT GTGCCAGCTG TTGGTCATGG GAAATGAAGA AACACTGGGA 
GGATTTCCAG TCAAGAGTGT TGTGCCAGCT TTGATAACAT TGCTGCAGAT GGAGCATAAC 
TTTGACATCA TGAACCATGC TTGTCGAGCC TTAACATACA TGATGGAAGC ACTTCCCAGA 
TCATCTGCTG TAGTGGTAGA TGCAATTCCT GTCTTCTTGG AAAAGCTGCA AGTTATTCAG 
TGCATTGATG TGGCAGAGCA GGCACTGACA GCCCTAGAGA TGCTATCACG CAGGCATAGT 
AAAGCCATTC TGCAGGCAGG TGGGTTGGCA GACTGCTTGC TGTATCTGGA ATTCTTCAGT 
ATAAATGCGC AGAGGAATGC ACTAGCTATT GCTGCCAATT GCTGCCAGAG TATAACACCT 
GATGAGTTTC ACTTTGTGGC AGACTCTTTG CCACTGCTTA CACAAAGGTT AACCCATCAG 
GACAAGAAGT CTGTTGAAAG CACTTGTCTC TGTTTTGCAC GGCTGGTAGA CAATTTCCAG 
CATGAGGAGA ACTTGCTGCA GCAGGTTGCT TCCAAGGACT TGTTAACAAA CATCCAGCAA 
CTCTTGGTAG TGACACCTCC TATCCTGAGC TCAGGAATGT TCATCATGGT GGTGCGCATG 
TTTTCCTTAA TGTGTTCCAA TTGTCCAACG CTTGCAGTCC AACTTATGAA GCAAAACATT 
GCAGAAACAC TTCACTTTCT CCTTTGTGGA GCCTCAAATG GGAGTTGTCA AGAACAAATT 
GACCTTGTTC CACGAAGTCC TCAAGAACTC TATGAGCTTA CTTCTCTTAT CTGTGAACTG 
ATGCCTTGTC TGCCAAAAGA GGGGATATTT GCTGTTGATA CTATGCTAAA GAAAGGAAAT 
GCACAAAATA CAGATGGTGC GATATGGCAA TGGCGAGATG ACAGGGGTCT CTGGCATCCC 
TATAACAGGA TTGATAGTCG AATAATAGAG GCAGCTCATC AGGTTGGTGA GGATGAGATA 
AGCCTGTCGA CACTTGGACG TGTCTATACT ATTGATTTTA ATTCTATGCA GCAAATAAAT 
GAGGATACTG GAACAGCACG TGCCATTCAG CGGAAACCAA ACCCTTTAGC CAATACAAAC 
ACTAGTGGAC ATTCAGAATT GAAGAAGGAT GATGCTCGAG CACAGCTAAT GAAAGAAGAC 
CCAGAATTGG CAAAATCCTT TATCAAAACA CTGTTTGGTG TTCTTTATGA AGTGTATAGT 
TCTTCAGCTG GACCTGCTGT TAGACACAAG TGCCTTAGAG CAATACTTAG AATAATTTAT 
TTTGCTGATG CTGAACTCCT GAAGGATGTG CTGAAAAACC ATGCTGTGTC AAGTCACATT 
GCCTCCATGC TGTCAAGTCA AGACCTTAAG ATAGTAGTTG GAGCCCTGCA GATGGCAGAA 
ATCTTAATGC AGAAGCTACC TGACATTTTT AGTGTTTACT TCAGAAGAGA AGGAGTGATG 
CATCAAGTGA AAAACTTAGC GGAGTCTGAG GCTTTGCTAA CGAGCCCACC AAAAGTATGC 
ACTAATGGAT CAGGAACACT GGGTACCACT ACAACTATAA GTACTGGAAC AGCCACTGCT 
GCCACTAATG CAGCTGCAGA TTTGGGCTCT CCCAGCTTAC AACACAGCCG GGAGGATTCT 
TTAGATCTGA GCCCACAGGG ACGACTGAGT GATGTTCTGA AGAGAAAAAG ACTGCCAAAA 
CGAGGGCCAA GGAGACCAAA ATATTCTCCC CCAAGAGATG ATGACAAAGT AGACAATCAA 
GCAAAAAGCC CCACGACTAC TCAGTCTCCT AAATCTTCTT TCTTAGCAAG TTTAAATCCC 
AAAACATGGG GAAGATTAAG TACACAGTCT AACAGTAATA ACATTGAACC AGCAAGAACA 
GCAGGTGTAA GTGGTCTTGC AAGAGCTGCT TCTAAGGATA CAATTTCCAA TAACAGAGAA 
AAAATTAAGG GTTGGATTAA GGAGCAAGCC CATAAATTTG TAGACCGTTA TTTTAGTTCG 
GAAAACATGG ATGGAAGCAA TCCTGCACTA AATGTATTAC AGAGACTTTG CACTGCAACT 
GAACAACTTA ACCTCCAGGT GGATGGTGGA ACAGAGTGCC TTGTAGAAAT CCGTAGCATT 
GTCTCGGAGT CTGACGTCTC CTCATTTGAA ATCCAGCATA GTGGATTTGT GAAACAACTG 
CTGCTTTATT TGACATCTAA AAGTGAGAAA GATGCTGTAA GCAGGGATAT CAGATTGAAA 
AGATTTCTTC ATGTATTTTT CTCCTCTCCA CTTCCTGGAG AAGAACCCCT TGGAAGATTA 
GAGCCATTAG AAAATGCACC TTTGTTGGCA TTAGTCCATA AAATGAACAA TTGCCTCAGT 
CAGATGGAGC AGTTTCCAGT GAAAGTGCAT GACTTCCCCA GTGGAAATGG AACAGGGAGC 
AGTTTTTCTC TTAACAGAGG ATCCCAAGCT TTAAAATTCT TCAATACGCA TCAGTTAAAA 
TGCCAGCTGC AAAGACATCC AGACTGTGCT AATGTGAAAC AGTGGAAGGG TGGACCTGTG 
AAGATTGATC CTCTGGCTTT GGTTCAAGCC ATTGAAAGAT ATCTTGTTGT TAGAGGTTAT 
GGAAGAGTTA GAGAAGATGA TGAGGATAGT GATGATGATG GGTCAGATGA AGAAATAGAT 
GAATCCTTGG CTGCTCAATT CTTAAATTCA GGGAATGTGA GACATAGATT GCAGTTTTAC 
ATTGGGGATC ACTTGCTACC ATATAATATG ACTGTATATC AAGCAGTTAA GCTGTACAGT 
TTGCAAGCTG AGGAGGAGAG GGAATCGACT GATGATGAAA GCAATCCATT AGGAAGAGCT 
GGGATTTGGA CAAAAACACA TACCATTTGG TACAAACCTG TACGGGAGGA TGAAGATGGC 
AACAAAGACT GTGTTGGTGG TAAAAGAGGA AGAGCTCAAA CTGCTCCCAC AAAAACCTCA 
CCTAGGAATT CTAAAAAGCA TGATGAGTTG TGGCACGATG GTGTATGCCC TTCAGTATTG 
AATCCTTTAG AAGTTTACCT CATATCTACT CCTCCTGAAA ACATAACCTT TGAAGATCCT 
TCATTAGATG TTATTCTTCT TTTGAGAGTT TTACATGCTA TCAGTCGATA CTGGTATTAT 
TTGTATGATA ATGCAATATG CAAGGAGATT ATTCCAACTT CGGAATTTAT CAATAGTAAA 
CTGACAGCAA AAGCAAACAG ACAGCTTCAA GATCCACTGG TAATTATGAC AGGAAACATA 
CCGACTTGGC TGACAGAGCT TGGAAAAACA TGCCCATTTT TCTTTCCATT TGATACCCGG 
CAAATGCTGT TTTATGTAAC TGCTTTTGAT CGTGATCGAG CCATGCAAAG ACTACTGGAT 
ACTAATCCAG AAATCAATCA ATCAGATTCT CAGGATAGCA GAGTGGCACC ACGACTGGAC 
AGGAAAAAAC GCACTGTGAA CAGAGATGAG CTGTTGAAAC AGGCAGAATC TGTGATGCAG 
GATCTAGGCA GTTCAAGAGC CATGTTGGAA ATCCAGTATG AGAATGAAGT TGGCACAGGC 
CTGGGCCCTA CACTAGAGTT CTATGCACTT GTATCTCAGG AACTACAGAG AGCAGACTTG 
GGCCTTTGGA GGGGAGAAGA AGTAACTTTA GCCAATCCAA AAGGAAGCCA AGAAGGTACC 
AAGTATATTC ATAATCTCCA AGGCCTTTTT GCACTTCCTT TTGGTAGAAC AGCCAAGCCT 
GCTCACATTG CAAAAGTTAA AATGAAGTTC CGCTTTCTGG GAAAACTAAT GGCTAAGGCA 
ATCATGGATT TTAGACTGGT GGATCTTCCT CTTGGACTTC CTTTTTATAA ATGGATGTTA 
CGCCAGGAAA CTTCTTTGAC GTCACATGAC TTGTTCAGTA TTGATCCAGT AGTAGCCAAA 
TCAATATATC ACCTTGAAGA TATTGTAAGA CAAAAGAAAA GACTTGAGCA GGACAAAACA 
CAGACCAAAG AAAGTCTACA GTATGCATTG GAAACCCTGA CTATGAATGG CTGCTCAGTG 
GAGGATCTAG GGCTGGATTT CACACTTCCT GGATTTCCTA ACATCGAGCT GAAAAAAGGG 
GGAAAAGATA CACCAGTCAC CATCCACAAC TTGGAGGAGT ACCTCAGATT GGTTATATTC 
TGGGCACTTA ACGAAGGTGT TGCCAGACAG TTTGATTCTT TCAGAGATGG ATTTGAATCA 
GTCTTCCCCC TCAGTCATCT TCAATACTTT TATCCTGAGG AGTTGGAGCA ACTCTTATGT 
GGCAGCAAAA CAGACACATG GGATGCCAAG ACCTTAATGG AATGTTGCAG GCCAGATCAC 
GGTTATACAC ATGACAGTCG AGCAGTGAAG TATCTCTTTG AAATTCTCAG TAGTTTTGAT 
AGTGAGCAGC AGAGACTGTT TCTTCAGTTT GTGACCGGTA GTCCCAGGCT GCCAGTAGGA 
GGCTTTCGAA GTTTGAATCC TCCATTGACA ATTGTACGGA AGACATTTGA GTCTACAGAG 
AATCCAGATG ACTTCTTGCC CTCAGTAATG ACTTGTGTGA ACTATCTCAA ATTGCCGGAC 
TATTCAACTA TTGAGATAAT GCGTGAAAAA CTCTTGATAG CTGCAAGAGA AGGGCAGCAG 
TCATTCCATC TTTCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015132454.1
CDS	73..6270
Translation	MSNRPNNNPGGSLRRSQRNTAGAQPQDDAAGGRSHLGQAKHKAHSPPESRKSISKTPKVQSNTTSEQSKGHFSKRGCSSSAVLIPQEDSERVSTSEKQKTGQVPKKDNSRGVKRSASPDYSRTNSPSSAKKPKALQHAETSSESNKPHTKSKRRHLDQEQQKSTQLPSTSKAHTRKGGAAGSSRSQKRKRTESTSCIKSRSAVESTGAEEKSAKSSKLASKSVTSAKAGCSTITDSSSSASTSSSSSAVASASSTVPQGARVKQGKDQNKARRSRSASSPSPRRSSRDKEQSKTGGSSKFDWAARFSPKVSLPKTKLSLPGSSKSETSKPGPSGLQAKLASLRKSTKKRSESPPAELPSLRRSTRQKTTGSCASTSRRGSGLGKRGAAEARRQEKMADPDNNQDGVNSSAARTDETPQGAAASSSVAGAVGMTTSGESESDDSEMGRLQALLEARGLPPHLFGPLGPRMSQLFHRTIGSGASSKAQQLLQGLQATDESQQLQAVIEMCQLLVMGNEETLGGFPVKSVVPALITLLQMEHNFDIMNHACRALTYMMEALPRSSAVVVDAIPVFLEKLQVIQCIDVAEQALTALEMLSRRHSKAILQAGGLADCLLYLEFFSINAQRNALAIAANCCQSITPDEFHFVADSLPLLTQRLTHQDKKSVESTCLCFARLVDNFQHEENLLQQVASKDLLTNIQQLLVVTPPILSSGMFIMVVRMFSLMCSNCPTLAVQLMKQNIAETLHFLLCGASNGSCQEQIDLVPRSPQELYELTSLICELMPCLPKEGIFAVDTMLKKGNAQNTDGAIWQWRDDRGLWHPYNRIDSRIIEAAHQVGEDEISLSTLGRVYTIDFNSMQQINEDTGTARAIQRKPNPLANTNTSGHSELKKDDARAQLMKEDPELAKSFIKTLFGVLYEVYSSSAGPAVRHKCLRAILRIIYFADAELLKDVLKNHAVSSHIASMLSSQDLKIVVGALQMAEILMQKLPDIFSVYFRREGVMHQVKNLAESEALLTSPPKVCTNGSGTLGTTTTISTGTATAATNAAADLGSPSLQHSREDSLDLSPQGRLSDVLKRKRLPKRGPRRPKYSPPRDDDKVDNQAKSPTTTQSPKSSFLASLNPKTWGRLSTQSNSNNIEPARTAGVSGLARAASKDTISNNREKIKGWIKEQAHKFVDRYFSSENMDGSNPALNVLQRLCTATEQLNLQVDGGTECLVEIRSIVSESDVSSFEIQHSGFVKQLLLYLTSKSEKDAVSRDIRLKRFLHVFFSSPLPGEEPLGRLEPLENAPLLALVHKMNNCLSQMEQFPVKVHDFPSGNGTGSSFSLNRGSQALKFFNTHQLKCQLQRHPDCANVKQWKGGPVKIDPLALVQAIERYLVVRGYGRVREDDEDSDDDGSDEEIDESLAAQFLNSGNVRHRLQFYIGDHLLPYNMTVYQAVKLYSLQAEEERESTDDESNPLGRAGIWTKTHTIWYKPVREDEDGNKDCVGGKRGRAQTAPTKTSPRNSKKHDELWHDGVCPSVLNPLEVYLISTPPENITFEDPSLDVILLLRVLHAISRYWYYLYDNAICKEIIPTSEFINSKLTAKANRQLQDPLVIMTGNIPTWLTELGKTCPFFFPFDTRQMLFYVTAFDRDRAMQRLLDTNPEINQSDSQDSRVAPRLDRKKRTVNRDELLKQAESVMQDLGSSRAMLEIQYENEVGTGLGPTLEFYALVSQELQRADLGLWRGEEVTLANPKGSQEGTKYIHNLQGLFALPFGRTAKPAHIAKVKMKFRFLGKLMAKAIMDFRLVDLPLGLPFYKWMLRQETSLTSHDLFSIDPVVAKSIYHLEDIVRQKKRLEQDKTQTKESLQYALETLTMNGCSVEDLGLDFTLPGFPNIELKKGGKDTPVTIHNLEEYLRLVIFWALNEGVARQFDSFRDGFESVFPLSHLQYFYPEELEQLLCGSKTDTWDAKTLMECCRPDHGYTHDSRAVKYLFEILSSFDSEQQRLFLQFVTGSPRLPVGGFRSLNPPLTIVRKTFESTENPDDFLPSVMTCVNYLKLPDYSTIEIMREKLLIAAREGQQSFHLS

Target ORF information:

RefSeq Version	XM_015276968.1
Organism	Gallus gallus(chicken)
Definition	Gallus gallus thyroid hormone receptor interactor 12 (TRIP12), transcript variant X6, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015276968.1

ORF Insert Sequence:

ATGTCCAACC GGCCTAATAA CAATCCAGGG GGGTCACTGC GACGTTCACA GAGGAACACT 
GCTGGGGCCC AGCCACAAGA CGACGCAGCA GGAGGAAGGT CACATTTAGG GCAGGCAAAA 
CATAAGGCAC ATAGCCCTCC TGAGAGTAGA AAATCTATTT CAAAGACACC CAAAGTGCAG 
TCTAATACTA CTTCTGAGCA GTCCAAGGGA CACTTTTCTA AAAGAGGCTG TAGTTCATCT 
GCTGTTTTAA TACCACAAGA AGATTCAGAA AGAGTCAGTA CTTCAGAAAA GCAAAAAACG 
GGGCAAGTGC CTAAGAAAGA CAATTCTCGA GGAGTTAAAC GCAGTGCTAG TCCAGATTAC 
AGCAGGACCA ATTCTCCTAG CTCTGCTAAA AAACCTAAAG CACTTCAACA CGCCGAAACT 
TCCTCGGAAA GTAACAAGCC ACATACTAAA TCCAAGAGAA GACACTTAGA CCAAGAACAG 
CAGAAGTCTA CACAATTGCC ATCAACAAGC AAGGCTCACA CCAGAAAGGG TGGAGCTGCT 
GGTAGCTCCC GAAGTCAGAA AAGGAAAAGG ACAGAGAGTA CGTCTTGTAT AAAGAGTCGT 
TCAGCAGTTG AATCAACTGG AGCTGAAGAG AAGTCAGCAA AATCCTCCAA GCTGGCTTCA 
AAATCGGTGA CCTCAGCCAA AGCTGGGTGT AGCACCATCA CTGATTCTTC TTCTTCAGCT 
TCTACATCCT CTTCCTCTTC TGCTGTTGCC TCTGCTTCTT CCACTGTTCC TCAGGGTGCC 
AGAGTGAAAC AGGGTAAGGA CCAGAATAAG GCTAGACGTT CACGTTCTGC GTCCAGCCCT 
AGTCCAAGAA GGAGCAGCAG GGACAAAGAA CAGAGCAAAA CAGGTGGCTC TTCAAAGTTT 
GACTGGGCTG CTCGATTCAG TCCAAAAGTT AGTCTCCCTA AAACAAAACT GTCTCTACCA 
GGCTCTTCTA AGTCAGAGAC CTCAAAACCT GGACCTTCAG GACTACAGGC TAAGCTAGCA 
AGCCTAAGAA AATCCACAAA GAAGCGCAGT GAATCACCAC CTGCTGAGCT CCCCAGTTTA 
CGGCGGAGCA CACGGCAAAA GACCACGGGC TCCTGTGCGA GTACCAGTCG GCGGGGCTCT 
GGCCTGGGCA AAAGAGGAGC AGCTGAAGCT CGCCGACAGG AGAAGATGGC TGATCCTGAC 
AACAACCAGG ATGGAGTTAA CTCTTCAGCT GCACGTACAG ATGAGACTCC CCAGGGAGCT 
GCAGCTTCTA GCTCTGTTGC TGGGGCTGTA GGTATGACAA CATCTGGAGA AAGTGAGTCA 
GATGACTCTG AGATGGGAAG ACTACAAGCT CTGTTAGAGG CTAGGGGTCT TCCTCCTCAC 
CTGTTTGGCC CTCTTGGTCC TCGGATGTCG CAGCTCTTCC ACAGGACAAT TGGAAGTGGA 
GCTAGTTCTA AAGCCCAGCA GCTTTTACAA GGTCTCCAAG CCACTGATGA AAGTCAGCAA 
CTACAGGCAG TTATTGAGAT GTGCCAGCTG TTGGTCATGG GAAATGAAGA AACACTGGGA 
GGATTTCCAG TCAAGAGTGT TGTGCCAGCT TTGATAACAT TGCTGCAGAT GGAGCATAAC 
TTTGACATCA TGAACCATGC TTGTCGAGCC TTAACATACA TGATGGAAGC ACTTCCCAGA 
TCATCTGCTG TAGTGGTAGA TGCAATTCCT GTCTTCTTGG AAAAGCTGCA AGTTATTCAG 
TGCATTGATG TGGCAGAGCA GGCACTGACA GCCCTAGAGA TGCTATCACG CAGGCATAGT 
AAAGCCATTC TGCAGGCAGG TGGGTTGGCA GACTGCTTGC TGTATCTGGA ATTCTTCAGT 
ATAAATGCGC AGAGGAATGC ACTAGCTATT GCTGCCAATT GCTGCCAGAG TATAACACCT 
GATGAGTTTC ACTTTGTGGC AGACTCTTTG CCACTGCTTA CACAAAGGTT AACCCATCAG 
GACAAGAAGT CTGTTGAAAG CACTTGTCTC TGTTTTGCAC GGCTGGTAGA CAATTTCCAG 
CATGAGGAGA ACTTGCTGCA GCAGGTTGCT TCCAAGGACT TGTTAACAAA CATCCAGCAA 
CTCTTGGTAG TGACACCTCC TATCCTGAGC TCAGGAATGT TCATCATGGT GGTGCGCATG 
TTTTCCTTAA TGTGTTCCAA TTGTCCAACG CTTGCAGTCC AACTTATGAA GCAAAACATT 
GCAGAAACAC TTCACTTTCT CCTTTGTGGA GCCTCAAATG GGAGTTGTCA AGAACAAATT 
GACCTTGTTC CACGAAGTCC TCAAGAACTC TATGAGCTTA CTTCTCTTAT CTGTGAACTG 
ATGCCTTGTC TGCCAAAAGA GGGGATATTT GCTGTTGATA CTATGCTAAA GAAAGGAAAT 
GCACAAAATA CAGATGGTGC GATATGGCAA TGGCGAGATG ACAGGGGTCT CTGGCATCCC 
TATAACAGGA TTGATAGTCG AATAATAGAG GCAGCTCATC AGGTTGGTGA GGATGAGATA 
AGCCTGTCGA CACTTGGACG TGTCTATACT ATTGATTTTA ATTCTATGCA GCAAATAAAT 
GAGGATACTG GAACAGCACG TGCCATTCAG CGGAAACCAA ACCCTTTAGC CAATACAAAC 
ACTAGTGGAC ATTCAGAATT GAAGAAGGAT GATGCTCGAG CACAGCTAAT GAAAGAAGAC 
CCAGAATTGG CAAAATCCTT TATCAAAACA CTGTTTGGTG TTCTTTATGA AGTGTATAGT 
TCTTCAGCTG GACCTGCTGT TAGACACAAG TGCCTTAGAG CAATACTTAG AATAATTTAT 
TTTGCTGATG CTGAACTCCT GAAGGATGTG CTGAAAAACC ATGCTGTGTC AAGTCACATT 
GCCTCCATGC TGTCAAGTCA AGACCTTAAG ATAGTAGTTG GAGCCCTGCA GATGGCAGAA 
ATCTTAATGC AGAAGCTACC TGACATTTTT AGTGTTTACT TCAGAAGAGA AGGAGTGATG 
CATCAAGTGA AAAACTTAGC GGAGTCTGAG GCTTTGCTAA CGAGCCCACC AAAAGTATGC 
ACTAATGGAT CAGGAACACT GGGTACCACT ACAACTATAA GTACTGGAAC AGCCACTGCT 
GCCACTAATG CAGCTGCAGA TTTGGGCTCT CCCAGCTTAC AACACAGCCG GGAGGATTCT 
TTAGATCTGA GCCCACAGGG ACGACTGAGT GATGTTCTGA AGAGAAAAAG ACTGCCAAAA 
CGAGGGCCAA GGAGACCAAA ATATTCTCCC CCAAGAGATG ATGACAAAGT AGACAATCAA 
GCAAAAAGCC CCACGACTAC TCAGTCTCCT AAATCTTCTT TCTTAGCAAG TTTAAATCCC 
AAAACATGGG GAAGATTAAG TACACAGTCT AACAGTAATA ACATTGAACC AGCAAGAACA 
GCAGGTGTAA GTGGTCTTGC AAGAGCTGCT TCTAAGGATA CAATTTCCAA TAACAGAGAA 
AAAATTAAGG GTTGGATTAA GGAGCAAGCC CATAAATTTG TAGACCGTTA TTTTAGTTCG 
GAAAACATGG ATGGAAGCAA TCCTGCACTA AATGTATTAC AGAGACTTTG CACTGCAACT 
GAACAACTTA ACCTCCAGGT GGATGGTGGA ACAGAGTGCC TTGTAGAAAT CCGTAGCATT 
GTCTCGGAGT CTGACGTCTC CTCATTTGAA ATCCAGCATA GTGGATTTGT GAAACAACTG 
CTGCTTTATT TGACATCTAA AAGTGAGAAA GATGCTGTAA GCAGGGATAT CAGATTGAAA 
AGATTTCTTC ATGTATTTTT CTCCTCTCCA CTTCCTGGAG AAGAACCCCT TGGAAGATTA 
GAGCCATTAG AAAATGCACC TTTGTTGGCA TTAGTCCATA AAATGAACAA TTGCCTCAGT 
CAGATGGAGC AGTTTCCAGT GAAAGTGCAT GACTTCCCCA GTGGAAATGG AACAGGGAGC 
AGTTTTTCTC TTAACAGAGG ATCCCAAGCT TTAAAATTCT TCAATACGCA TCAGTTAAAA 
TGCCAGCTGC AAAGACATCC AGACTGTGCT AATGTGAAAC AGTGGAAGGG TGGACCTGTG 
AAGATTGATC CTCTGGCTTT GGTTCAAGCC ATTGAAAGAT ATCTTGTTGT TAGAGGTTAT 
GGAAGAGTTA GAGAAGATGA TGAGGATAGT GATGATGATG GGTCAGATGA AGAAATAGAT 
GAATCCTTGG CTGCTCAATT CTTAAATTCA GGGAATGTGA GACATAGATT GCAGTTTTAC 
ATTGGGGATC ACTTGCTACC ATATAATATG ACTGTATATC AAGCAGTTAA GCTGTACAGT 
TTGCAAGCTG AGGAGGAGAG GGAATCGACT GATGATGAAA GCAATCCATT AGGAAGAGCT 
GGGATTTGGA CAAAAACACA TACCATTTGG TACAAACCTG TACGGGAGGA TGAAGATGGC 
AACAAAGACT GTGTTGGTGG TAAAAGAGGA AGAGCTCAAA CTGCTCCCAC AAAAACCTCA 
CCTAGGAATT CTAAAAAGCA TGATGAGTTG TGGCACGATG GTGTATGCCC TTCAGTATTG 
AATCCTTTAG AAGTTTACCT CATATCTACT CCTCCTGAAA ACATAACCTT TGAAGATCCT 
TCATTAGATG TTATTCTTCT TTTGAGAGTT TTACATGCTA TCAGTCGATA CTGGTATTAT 
TTGTATGATA ATGCAATATG CAAGGAGATT ATTCCAACTT CGGAATTTAT CAATAGTAAA 
CTGACAGCAA AAGCAAACAG ACAGCTTCAA GATCCACTGG TAATTATGAC AGGAAACATA 
CCGACTTGGC TGACAGAGCT TGGAAAAACA TGCCCATTTT TCTTTCCATT TGATACCCGG 
CAAATGCTGT TTTATGTAAC TGCTTTTGAT CGTGATCGAG CCATGCAAAG ACTACTGGAT 
ACTAATCCAG AAATCAATCA ATCAGATTCT CAGGATAGCA GAGTGGCACC ACGACTGGAC 
AGGAAAAAAC GCACTGTGAA CAGAGATGAG CTGTTGAAAC AGGCAGAATC TGTGATGCAG 
GATCTAGGCA GTTCAAGAGC CATGTTGGAA ATCCAGTATG AGAATGAAGT TGGCACAGGC 
CTGGGCCCTA CACTAGAGTT CTATGCACTT GTATCTCAGG AACTACAGAG AGCAGACTTG 
GGCCTTTGGA GGGGAGAAGA AGTAACTTTA GCCAATCCAA AAGGAAGCCA AGAAGGTACC 
AAGTATATTC ATAATCTCCA AGGCCTTTTT GCACTTCCTT TTGGTAGAAC AGCCAAGCCT 
GCTCACATTG CAAAAGTTAA AATGAAGTTC CGCTTTCTGG GAAAACTAAT GGCTAAGGCA 
ATCATGGATT TTAGACTGGT GGATCTTCCT CTTGGACTTC CTTTTTATAA ATGGATGTTA 
CGCCAGGAAA CTTCTTTGAC GTCACATGAC TTGTTCAGTA TTGATCCAGT AGTAGCCAAA 
TCAATATATC ACCTTGAAGA TATTGTAAGA CAAAAGAAAA GACTTGAGCA GGACAAAACA 
CAGACCAAAG AAAGTCTACA GTATGCATTG GAAACCCTGA CTATGAATGG CTGCTCAGTG 
GAGGATCTAG GGCTGGATTT CACACTTCCT GGATTTCCTA ACATCGAGCT GAAAAAAGGG 
GGAAAAGATA CACCAGTCAC CATCCACAAC TTGGAGGAGT ACCTCAGATT GGTTATATTC 
TGGGCACTTA ACGAAGGTGT TGCCAGACAG TTTGATTCTT TCAGAGATGG ATTTGAATCA 
GTCTTCCCCC TCAGTCATCT TCAATACTTT TATCCTGAGG AGTTGGAGCA ACTCTTATGT 
GGCAGCAAAA CAGACACATG GGATGCCAAG ACCTTAATGG AATGTTGCAG GCCAGATCAC 
GGTTATACAC ATGACAGTCG AGCAGTGAAG TATCTCTTTG AAATTCTCAG TAGTTTTGAT 
AGTGAGCAGC AGAGACTGTT TCTTCAGTTT GTGACCGGTA GTCCCAGGCT GCCAGTAGGA 
GGCTTTCGAA GTTTGAATCC TCCATTGACA ATTGTACGGA AGACATTTGA GTCTACAGAG 
AATCCAGATG ACTTCTTGCC CTCAGTAATG ACTTGTGTGA ACTATCTCAA ATTGCCGGAC 
TATTCAACTA TTGAGATAAT GCGTGAAAAA CTCTTGATAG CTGCAAGAGA AGGGCAGCAG 
TCATTCCATC TTTCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TRIP12 ( XM_004937064.3 ) cDNA ORF clone, Gallus gallus(chicken) -> XP_004937121.1 Gallus gallus thyroid hormone receptor interactor 12 (TRIP12), transcript variant X1, mRNA.

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CloneID	OGa12502
Clone ID Related Accession (Same CDS sequence)	XM_015276964.1 , XM_015276968.2 , XM_015276967.1 , XM_015276968.1 , XM_004937064.3 , XM_015276964.2 , XM_015276966.2 , XM_004937064.2 , XM_015276966.1 , XM_015276967.2 , XM_015276965.1
Accession Version	XM_004937064.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6198bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-16
Organism	Gallus gallus(chicken)
Product	E3 ubiquitin-protein ligase TRIP12 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006096.5) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On May 17, 2018 this sequence version replaced XM_004937064.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Gallus gallus Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCCAACC GGCCTAATAA CAATCCAGGG GGGTCACTGC GACGTTCACA GAGGAACACT 
GCTGGGGCCC AGCCACAAGA CGACGCAGCA GGAGGAAGGT CACATTTAGG GCAGGCAAAA 
CATAAGGCAC ATAGCCCTCC TGAGAGTAGA AAATCTATTT CAAAGACACC CAAAGTGCAG 
TCTAATACTA CTTCTGAGCA GTCCAAGGGA CACTTTTCTA AAAGAGGCTG TAGTTCATCT 
GCTGTTTTAA TACCACAAGA AGATTCAGAA AGAGTCAGTA CTTCAGAAAA GCAAAAAACG 
GGGCAAGTGC CTAAGAAAGA CAATTCTCGA GGAGTTAAAC GCAGTGCTAG TCCAGATTAC 
AGCAGGACCA ATTCTCCTAG CTCTGCTAAA AAACCTAAAG CACTTCAACA CGCCGAAACT 
TCCTCGGAAA GTAACAAGCC ACATACTAAA TCCAAGAGAA GACACTTAGA CCAAGAACAG 
CAGAAGTCTA CACAATTGCC ATCAACAAGC AAGGCTCACA CCAGAAAGGG TGGAGCTGCT 
GGTAGCTCCC GAAGTCAGAA AAGGAAAAGG ACAGAGAGTA CGTCTTGTAT AAAGAGTCGT 
TCAGCAGTTG AATCAACTGG AGCTGAAGAG AAGTCAGCAA AATCCTCCAA GCTGGCTTCA 
AAATCGGTGA CCTCAGCCAA AGCTGGGTGT AGCACCATCA CTGATTCTTC TTCTTCAGCT 
TCTACATCCT CTTCCTCTTC TGCTGTTGCC TCTGCTTCTT CCACTGTTCC TCAGGGTGCC 
AGAGTGAAAC AGGGTAAGGA CCAGAATAAG GCTAGACGTT CACGTTCTGC GTCCAGCCCT 
AGTCCAAGAA GGAGCAGCAG GGACAAAGAA CAGAGCAAAA CAGGTGGCTC TTCAAAGTTT 
GACTGGGCTG CTCGATTCAG TCCAAAAGTT AGTCTCCCTA AAACAAAACT GTCTCTACCA 
GGCTCTTCTA AGTCAGAGAC CTCAAAACCT GGACCTTCAG GACTACAGGC TAAGCTAGCA 
AGCCTAAGAA AATCCACAAA GAAGCGCAGT GAATCACCAC CTGCTGAGCT CCCCAGTTTA 
CGGCGGAGCA CACGGCAAAA GACCACGGGC TCCTGTGCGA GTACCAGTCG GCGGGGCTCT 
GGCCTGGGCA AAAGAGGAGC AGCTGAAGCT CGCCGACAGG AGAAGATGGC TGATCCTGAC 
AACAACCAGG ATGGAGTTAA CTCTTCAGCT GCACGTACAG ATGAGACTCC CCAGGGAGCT 
GCAGCTTCTA GCTCTGTTGC TGGGGCTGTA GGTATGACAA CATCTGGAGA AAGTGAGTCA 
GATGACTCTG AGATGGGAAG ACTACAAGCT CTGTTAGAGG CTAGGGGTCT TCCTCCTCAC 
CTGTTTGGCC CTCTTGGTCC TCGGATGTCG CAGCTCTTCC ACAGGACAAT TGGAAGTGGA 
GCTAGTTCTA AAGCCCAGCA GCTTTTACAA GGTCTCCAAG CCACTGATGA AAGTCAGCAA 
CTACAGGCAG TTATTGAGAT GTGCCAGCTG TTGGTCATGG GAAATGAAGA AACACTGGGA 
GGATTTCCAG TCAAGAGTGT TGTGCCAGCT TTGATAACAT TGCTGCAGAT GGAGCATAAC 
TTTGACATCA TGAACCATGC TTGTCGAGCC TTAACATACA TGATGGAAGC ACTTCCCAGA 
TCATCTGCTG TAGTGGTAGA TGCAATTCCT GTCTTCTTGG AAAAGCTGCA AGTTATTCAG 
TGCATTGATG TGGCAGAGCA GGCACTGACA GCCCTAGAGA TGCTATCACG CAGGCATAGT 
AAAGCCATTC TGCAGGCAGG TGGGTTGGCA GACTGCTTGC TGTATCTGGA ATTCTTCAGT 
ATAAATGCGC AGAGGAATGC ACTAGCTATT GCTGCCAATT GCTGCCAGAG TATAACACCT 
GATGAGTTTC ACTTTGTGGC AGACTCTTTG CCACTGCTTA CACAAAGGTT AACCCATCAG 
GACAAGAAGT CTGTTGAAAG CACTTGTCTC TGTTTTGCAC GGCTGGTAGA CAATTTCCAG 
CATGAGGAGA ACTTGCTGCA GCAGGTTGCT TCCAAGGACT TGTTAACAAA CATCCAGCAA 
CTCTTGGTAG TGACACCTCC TATCCTGAGC TCAGGAATGT TCATCATGGT GGTGCGCATG 
TTTTCCTTAA TGTGTTCCAA TTGTCCAACG CTTGCAGTCC AACTTATGAA GCAAAACATT 
GCAGAAACAC TTCACTTTCT CCTTTGTGGA GCCTCAAATG GGAGTTGTCA AGAACAAATT 
GACCTTGTTC CACGAAGTCC TCAAGAACTC TATGAGCTTA CTTCTCTTAT CTGTGAACTG 
ATGCCTTGTC TGCCAAAAGA GGGGATATTT GCTGTTGATA CTATGCTAAA GAAAGGAAAT 
GCACAAAATA CAGATGGTGC GATATGGCAA TGGCGAGATG ACAGGGGTCT CTGGCATCCC 
TATAACAGGA TTGATAGTCG AATAATAGAG GCAGCTCATC AGGTTGGTGA GGATGAGATA 
AGCCTGTCGA CACTTGGACG TGTCTATACT ATTGATTTTA ATTCTATGCA GCAAATAAAT 
GAGGATACTG GAACAGCACG TGCCATTCAG CGGAAACCAA ACCCTTTAGC CAATACAAAC 
ACTAGTGGAC ATTCAGAATT GAAGAAGGAT GATGCTCGAG CACAGCTAAT GAAAGAAGAC 
CCAGAATTGG CAAAATCCTT TATCAAAACA CTGTTTGGTG TTCTTTATGA AGTGTATAGT 
TCTTCAGCTG GACCTGCTGT TAGACACAAG TGCCTTAGAG CAATACTTAG AATAATTTAT 
TTTGCTGATG CTGAACTCCT GAAGGATGTG CTGAAAAACC ATGCTGTGTC AAGTCACATT 
GCCTCCATGC TGTCAAGTCA AGACCTTAAG ATAGTAGTTG GAGCCCTGCA GATGGCAGAA 
ATCTTAATGC AGAAGCTACC TGACATTTTT AGTGTTTACT TCAGAAGAGA AGGAGTGATG 
CATCAAGTGA AAAACTTAGC GGAGTCTGAG GCTTTGCTAA CGAGCCCACC AAAAGTATGC 
ACTAATGGAT CAGGAACACT GGGTACCACT ACAACTATAA GTACTGGAAC AGCCACTGCT 
GCCACTAATG CAGCTGCAGA TTTGGGCTCT CCCAGCTTAC AACACAGCCG GGAGGATTCT 
TTAGATCTGA GCCCACAGGG ACGACTGAGT GATGTTCTGA AGAGAAAAAG ACTGCCAAAA 
CGAGGGCCAA GGAGACCAAA ATATTCTCCC CCAAGAGATG ATGACAAAGT AGACAATCAA 
GCAAAAAGCC CCACGACTAC TCAGTCTCCT AAATCTTCTT TCTTAGCAAG TTTAAATCCC 
AAAACATGGG GAAGATTAAG TACACAGTCT AACAGTAATA ACATTGAACC AGCAAGAACA 
GCAGGTGTAA GTGGTCTTGC AAGAGCTGCT TCTAAGGATA CAATTTCCAA TAACAGAGAA 
AAAATTAAGG GTTGGATTAA GGAGCAAGCC CATAAATTTG TAGACCGTTA TTTTAGTTCG 
GAAAACATGG ATGGAAGCAA TCCTGCACTA AATGTATTAC AGAGACTTTG CACTGCAACT 
GAACAACTTA ACCTCCAGGT GGATGGTGGA ACAGAGTGCC TTGTAGAAAT CCGTAGCATT 
GTCTCGGAGT CTGACGTCTC CTCATTTGAA ATCCAGCATA GTGGATTTGT GAAACAACTG 
CTGCTTTATT TGACATCTAA AAGTGAGAAA GATGCTGTAA GCAGGGATAT CAGATTGAAA 
AGATTTCTTC ATGTATTTTT CTCCTCTCCA CTTCCTGGAG AAGAACCCCT TGGAAGATTA 
GAGCCATTAG AAAATGCACC TTTGTTGGCA TTAGTCCATA AAATGAACAA TTGCCTCAGT 
CAGATGGAGC AGTTTCCAGT GAAAGTGCAT GACTTCCCCA GTGGAAATGG AACAGGGAGC 
AGTTTTTCTC TTAACAGAGG ATCCCAAGCT TTAAAATTCT TCAATACGCA TCAGTTAAAA 
TGCCAGCTGC AAAGACATCC AGACTGTGCT AATGTGAAAC AGTGGAAGGG TGGACCTGTG 
AAGATTGATC CTCTGGCTTT GGTTCAAGCC ATTGAAAGAT ATCTTGTTGT TAGAGGTTAT 
GGAAGAGTTA GAGAAGATGA TGAGGATAGT GATGATGATG GGTCAGATGA AGAAATAGAT 
GAATCCTTGG CTGCTCAATT CTTAAATTCA GGGAATGTGA GACATAGATT GCAGTTTTAC 
ATTGGGGATC ACTTGCTACC ATATAATATG ACTGTATATC AAGCAGTTAA GCTGTACAGT 
TTGCAAGCTG AGGAGGAGAG GGAATCGACT GATGATGAAA GCAATCCATT AGGAAGAGCT 
GGGATTTGGA CAAAAACACA TACCATTTGG TACAAACCTG TACGGGAGGA TGAAGATGGC 
AACAAAGACT GTGTTGGTGG TAAAAGAGGA AGAGCTCAAA CTGCTCCCAC AAAAACCTCA 
CCTAGGAATT CTAAAAAGCA TGATGAGTTG TGGCACGATG GTGTATGCCC TTCAGTATTG 
AATCCTTTAG AAGTTTACCT CATATCTACT CCTCCTGAAA ACATAACCTT TGAAGATCCT 
TCATTAGATG TTATTCTTCT TTTGAGAGTT TTACATGCTA TCAGTCGATA CTGGTATTAT 
TTGTATGATA ATGCAATATG CAAGGAGATT ATTCCAACTT CGGAATTTAT CAATAGTAAA 
CTGACAGCAA AAGCAAACAG ACAGCTTCAA GATCCACTGG TAATTATGAC AGGAAACATA 
CCGACTTGGC TGACAGAGCT TGGAAAAACA TGCCCATTTT TCTTTCCATT TGATACCCGG 
CAAATGCTGT TTTATGTAAC TGCTTTTGAT CGTGATCGAG CCATGCAAAG ACTACTGGAT 
ACTAATCCAG AAATCAATCA ATCAGATTCT CAGGATAGCA GAGTGGCACC ACGACTGGAC 
AGGAAAAAAC GCACTGTGAA CAGAGATGAG CTGTTGAAAC AGGCAGAATC TGTGATGCAG 
GATCTAGGCA GTTCAAGAGC CATGTTGGAA ATCCAGTATG AGAATGAAGT TGGCACAGGC 
CTGGGCCCTA CACTAGAGTT CTATGCACTT GTATCTCAGG AACTACAGAG AGCAGACTTG 
GGCCTTTGGA GGGGAGAAGA AGTAACTTTA GCCAATCCAA AAGGAAGCCA AGAAGGTACC 
AAGTATATTC ATAATCTCCA AGGCCTTTTT GCACTTCCTT TTGGTAGAAC AGCCAAGCCT 
GCTCACATTG CAAAAGTTAA AATGAAGTTC CGCTTTCTGG GAAAACTAAT GGCTAAGGCA 
ATCATGGATT TTAGACTGGT GGATCTTCCT CTTGGACTTC CTTTTTATAA ATGGATGTTA 
CGCCAGGAAA CTTCTTTGAC GTCACATGAC TTGTTCAGTA TTGATCCAGT AGTAGCCAAA 
TCAATATATC ACCTTGAAGA TATTGTAAGA CAAAAGAAAA GACTTGAGCA GGACAAAACA 
CAGACCAAAG AAAGTCTACA GTATGCATTG GAAACCCTGA CTATGAATGG CTGCTCAGTG 
GAGGATCTAG GGCTGGATTT CACACTTCCT GGATTTCCTA ACATCGAGCT GAAAAAAGGG 
GGAAAAGATA CACCAGTCAC CATCCACAAC TTGGAGGAGT ACCTCAGATT GGTTATATTC 
TGGGCACTTA ACGAAGGTGT TGCCAGACAG TTTGATTCTT TCAGAGATGG ATTTGAATCA 
GTCTTCCCCC TCAGTCATCT TCAATACTTT TATCCTGAGG AGTTGGAGCA ACTCTTATGT 
GGCAGCAAAA CAGACACATG GGATGCCAAG ACCTTAATGG AATGTTGCAG GCCAGATCAC 
GGTTATACAC ATGACAGTCG AGCAGTGAAG TATCTCTTTG AAATTCTCAG TAGTTTTGAT 
AGTGAGCAGC AGAGACTGTT TCTTCAGTTT GTGACCGGTA GTCCCAGGCT GCCAGTAGGA 
GGCTTTCGAA GTTTGAATCC TCCATTGACA ATTGTACGGA AGACATTTGA GTCTACAGAG 
AATCCAGATG ACTTCTTGCC CTCAGTAATG ACTTGTGTGA ACTATCTCAA ATTGCCGGAC 
TATTCAACTA TTGAGATAAT GCGTGAAAAA CTCTTGATAG CTGCAAGAGA AGGGCAGCAG 
TCATTCCATC TTTCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_004937121.1
CDS	134..6331
Translation	MSNRPNNNPGGSLRRSQRNTAGAQPQDDAAGGRSHLGQAKHKAHSPPESRKSISKTPKVQSNTTSEQSKGHFSKRGCSSSAVLIPQEDSERVSTSEKQKTGQVPKKDNSRGVKRSASPDYSRTNSPSSAKKPKALQHAETSSESNKPHTKSKRRHLDQEQQKSTQLPSTSKAHTRKGGAAGSSRSQKRKRTESTSCIKSRSAVESTGAEEKSAKSSKLASKSVTSAKAGCSTITDSSSSASTSSSSSAVASASSTVPQGARVKQGKDQNKARRSRSASSPSPRRSSRDKEQSKTGGSSKFDWAARFSPKVSLPKTKLSLPGSSKSETSKPGPSGLQAKLASLRKSTKKRSESPPAELPSLRRSTRQKTTGSCASTSRRGSGLGKRGAAEARRQEKMADPDNNQDGVNSSAARTDETPQGAAASSSVAGAVGMTTSGESESDDSEMGRLQALLEARGLPPHLFGPLGPRMSQLFHRTIGSGASSKAQQLLQGLQATDESQQLQAVIEMCQLLVMGNEETLGGFPVKSVVPALITLLQMEHNFDIMNHACRALTYMMEALPRSSAVVVDAIPVFLEKLQVIQCIDVAEQALTALEMLSRRHSKAILQAGGLADCLLYLEFFSINAQRNALAIAANCCQSITPDEFHFVADSLPLLTQRLTHQDKKSVESTCLCFARLVDNFQHEENLLQQVASKDLLTNIQQLLVVTPPILSSGMFIMVVRMFSLMCSNCPTLAVQLMKQNIAETLHFLLCGASNGSCQEQIDLVPRSPQELYELTSLICELMPCLPKEGIFAVDTMLKKGNAQNTDGAIWQWRDDRGLWHPYNRIDSRIIEAAHQVGEDEISLSTLGRVYTIDFNSMQQINEDTGTARAIQRKPNPLANTNTSGHSELKKDDARAQLMKEDPELAKSFIKTLFGVLYEVYSSSAGPAVRHKCLRAILRIIYFADAELLKDVLKNHAVSSHIASMLSSQDLKIVVGALQMAEILMQKLPDIFSVYFRREGVMHQVKNLAESEALLTSPPKVCTNGSGTLGTTTTISTGTATAATNAAADLGSPSLQHSREDSLDLSPQGRLSDVLKRKRLPKRGPRRPKYSPPRDDDKVDNQAKSPTTTQSPKSSFLASLNPKTWGRLSTQSNSNNIEPARTAGVSGLARAASKDTISNNREKIKGWIKEQAHKFVDRYFSSENMDGSNPALNVLQRLCTATEQLNLQVDGGTECLVEIRSIVSESDVSSFEIQHSGFVKQLLLYLTSKSEKDAVSRDIRLKRFLHVFFSSPLPGEEPLGRLEPLENAPLLALVHKMNNCLSQMEQFPVKVHDFPSGNGTGSSFSLNRGSQALKFFNTHQLKCQLQRHPDCANVKQWKGGPVKIDPLALVQAIERYLVVRGYGRVREDDEDSDDDGSDEEIDESLAAQFLNSGNVRHRLQFYIGDHLLPYNMTVYQAVKLYSLQAEEERESTDDESNPLGRAGIWTKTHTIWYKPVREDEDGNKDCVGGKRGRAQTAPTKTSPRNSKKHDELWHDGVCPSVLNPLEVYLISTPPENITFEDPSLDVILLLRVLHAISRYWYYLYDNAICKEIIPTSEFINSKLTAKANRQLQDPLVIMTGNIPTWLTELGKTCPFFFPFDTRQMLFYVTAFDRDRAMQRLLDTNPEINQSDSQDSRVAPRLDRKKRTVNRDELLKQAESVMQDLGSSRAMLEIQYENEVGTGLGPTLEFYALVSQELQRADLGLWRGEEVTLANPKGSQEGTKYIHNLQGLFALPFGRTAKPAHIAKVKMKFRFLGKLMAKAIMDFRLVDLPLGLPFYKWMLRQETSLTSHDLFSIDPVVAKSIYHLEDIVRQKKRLEQDKTQTKESLQYALETLTMNGCSVEDLGLDFTLPGFPNIELKKGGKDTPVTIHNLEEYLRLVIFWALNEGVARQFDSFRDGFESVFPLSHLQYFYPEELEQLLCGSKTDTWDAKTLMECCRPDHGYTHDSRAVKYLFEILSSFDSEQQRLFLQFVTGSPRLPVGGFRSLNPPLTIVRKTFESTENPDDFLPSVMTCVNYLKLPDYSTIEIMREKLLIAAREGQQSFHLS

Target ORF information:

RefSeq Version	XM_004937064.3
Organism	Gallus gallus(chicken)
Definition	Gallus gallus thyroid hormone receptor interactor 12 (TRIP12), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_004937064.3

ORF Insert Sequence:

ATGTCCAACC GGCCTAATAA CAATCCAGGG GGGTCACTGC GACGTTCACA GAGGAACACT 
GCTGGGGCCC AGCCACAAGA CGACGCAGCA GGAGGAAGGT CACATTTAGG GCAGGCAAAA 
CATAAGGCAC ATAGCCCTCC TGAGAGTAGA AAATCTATTT CAAAGACACC CAAAGTGCAG 
TCTAATACTA CTTCTGAGCA GTCCAAGGGA CACTTTTCTA AAAGAGGCTG TAGTTCATCT 
GCTGTTTTAA TACCACAAGA AGATTCAGAA AGAGTCAGTA CTTCAGAAAA GCAAAAAACG 
GGGCAAGTGC CTAAGAAAGA CAATTCTCGA GGAGTTAAAC GCAGTGCTAG TCCAGATTAC 
AGCAGGACCA ATTCTCCTAG CTCTGCTAAA AAACCTAAAG CACTTCAACA CGCCGAAACT 
TCCTCGGAAA GTAACAAGCC ACATACTAAA TCCAAGAGAA GACACTTAGA CCAAGAACAG 
CAGAAGTCTA CACAATTGCC ATCAACAAGC AAGGCTCACA CCAGAAAGGG TGGAGCTGCT 
GGTAGCTCCC GAAGTCAGAA AAGGAAAAGG ACAGAGAGTA CGTCTTGTAT AAAGAGTCGT 
TCAGCAGTTG AATCAACTGG AGCTGAAGAG AAGTCAGCAA AATCCTCCAA GCTGGCTTCA 
AAATCGGTGA CCTCAGCCAA AGCTGGGTGT AGCACCATCA CTGATTCTTC TTCTTCAGCT 
TCTACATCCT CTTCCTCTTC TGCTGTTGCC TCTGCTTCTT CCACTGTTCC TCAGGGTGCC 
AGAGTGAAAC AGGGTAAGGA CCAGAATAAG GCTAGACGTT CACGTTCTGC GTCCAGCCCT 
AGTCCAAGAA GGAGCAGCAG GGACAAAGAA CAGAGCAAAA CAGGTGGCTC TTCAAAGTTT 
GACTGGGCTG CTCGATTCAG TCCAAAAGTT AGTCTCCCTA AAACAAAACT GTCTCTACCA 
GGCTCTTCTA AGTCAGAGAC CTCAAAACCT GGACCTTCAG GACTACAGGC TAAGCTAGCA 
AGCCTAAGAA AATCCACAAA GAAGCGCAGT GAATCACCAC CTGCTGAGCT CCCCAGTTTA 
CGGCGGAGCA CACGGCAAAA GACCACGGGC TCCTGTGCGA GTACCAGTCG GCGGGGCTCT 
GGCCTGGGCA AAAGAGGAGC AGCTGAAGCT CGCCGACAGG AGAAGATGGC TGATCCTGAC 
AACAACCAGG ATGGAGTTAA CTCTTCAGCT GCACGTACAG ATGAGACTCC CCAGGGAGCT 
GCAGCTTCTA GCTCTGTTGC TGGGGCTGTA GGTATGACAA CATCTGGAGA AAGTGAGTCA 
GATGACTCTG AGATGGGAAG ACTACAAGCT CTGTTAGAGG CTAGGGGTCT TCCTCCTCAC 
CTGTTTGGCC CTCTTGGTCC TCGGATGTCG CAGCTCTTCC ACAGGACAAT TGGAAGTGGA 
GCTAGTTCTA AAGCCCAGCA GCTTTTACAA GGTCTCCAAG CCACTGATGA AAGTCAGCAA 
CTACAGGCAG TTATTGAGAT GTGCCAGCTG TTGGTCATGG GAAATGAAGA AACACTGGGA 
GGATTTCCAG TCAAGAGTGT TGTGCCAGCT TTGATAACAT TGCTGCAGAT GGAGCATAAC 
TTTGACATCA TGAACCATGC TTGTCGAGCC TTAACATACA TGATGGAAGC ACTTCCCAGA 
TCATCTGCTG TAGTGGTAGA TGCAATTCCT GTCTTCTTGG AAAAGCTGCA AGTTATTCAG 
TGCATTGATG TGGCAGAGCA GGCACTGACA GCCCTAGAGA TGCTATCACG CAGGCATAGT 
AAAGCCATTC TGCAGGCAGG TGGGTTGGCA GACTGCTTGC TGTATCTGGA ATTCTTCAGT 
ATAAATGCGC AGAGGAATGC ACTAGCTATT GCTGCCAATT GCTGCCAGAG TATAACACCT 
GATGAGTTTC ACTTTGTGGC AGACTCTTTG CCACTGCTTA CACAAAGGTT AACCCATCAG 
GACAAGAAGT CTGTTGAAAG CACTTGTCTC TGTTTTGCAC GGCTGGTAGA CAATTTCCAG 
CATGAGGAGA ACTTGCTGCA GCAGGTTGCT TCCAAGGACT TGTTAACAAA CATCCAGCAA 
CTCTTGGTAG TGACACCTCC TATCCTGAGC TCAGGAATGT TCATCATGGT GGTGCGCATG 
TTTTCCTTAA TGTGTTCCAA TTGTCCAACG CTTGCAGTCC AACTTATGAA GCAAAACATT 
GCAGAAACAC TTCACTTTCT CCTTTGTGGA GCCTCAAATG GGAGTTGTCA AGAACAAATT 
GACCTTGTTC CACGAAGTCC TCAAGAACTC TATGAGCTTA CTTCTCTTAT CTGTGAACTG 
ATGCCTTGTC TGCCAAAAGA GGGGATATTT GCTGTTGATA CTATGCTAAA GAAAGGAAAT 
GCACAAAATA CAGATGGTGC GATATGGCAA TGGCGAGATG ACAGGGGTCT CTGGCATCCC 
TATAACAGGA TTGATAGTCG AATAATAGAG GCAGCTCATC AGGTTGGTGA GGATGAGATA 
AGCCTGTCGA CACTTGGACG TGTCTATACT ATTGATTTTA ATTCTATGCA GCAAATAAAT 
GAGGATACTG GAACAGCACG TGCCATTCAG CGGAAACCAA ACCCTTTAGC CAATACAAAC 
ACTAGTGGAC ATTCAGAATT GAAGAAGGAT GATGCTCGAG CACAGCTAAT GAAAGAAGAC 
CCAGAATTGG CAAAATCCTT TATCAAAACA CTGTTTGGTG TTCTTTATGA AGTGTATAGT 
TCTTCAGCTG GACCTGCTGT TAGACACAAG TGCCTTAGAG CAATACTTAG AATAATTTAT 
TTTGCTGATG CTGAACTCCT GAAGGATGTG CTGAAAAACC ATGCTGTGTC AAGTCACATT 
GCCTCCATGC TGTCAAGTCA AGACCTTAAG ATAGTAGTTG GAGCCCTGCA GATGGCAGAA 
ATCTTAATGC AGAAGCTACC TGACATTTTT AGTGTTTACT TCAGAAGAGA AGGAGTGATG 
CATCAAGTGA AAAACTTAGC GGAGTCTGAG GCTTTGCTAA CGAGCCCACC AAAAGTATGC 
ACTAATGGAT CAGGAACACT GGGTACCACT ACAACTATAA GTACTGGAAC AGCCACTGCT 
GCCACTAATG CAGCTGCAGA TTTGGGCTCT CCCAGCTTAC AACACAGCCG GGAGGATTCT 
TTAGATCTGA GCCCACAGGG ACGACTGAGT GATGTTCTGA AGAGAAAAAG ACTGCCAAAA 
CGAGGGCCAA GGAGACCAAA ATATTCTCCC CCAAGAGATG ATGACAAAGT AGACAATCAA 
GCAAAAAGCC CCACGACTAC TCAGTCTCCT AAATCTTCTT TCTTAGCAAG TTTAAATCCC 
AAAACATGGG GAAGATTAAG TACACAGTCT AACAGTAATA ACATTGAACC AGCAAGAACA 
GCAGGTGTAA GTGGTCTTGC AAGAGCTGCT TCTAAGGATA CAATTTCCAA TAACAGAGAA 
AAAATTAAGG GTTGGATTAA GGAGCAAGCC CATAAATTTG TAGACCGTTA TTTTAGTTCG 
GAAAACATGG ATGGAAGCAA TCCTGCACTA AATGTATTAC AGAGACTTTG CACTGCAACT 
GAACAACTTA ACCTCCAGGT GGATGGTGGA ACAGAGTGCC TTGTAGAAAT CCGTAGCATT 
GTCTCGGAGT CTGACGTCTC CTCATTTGAA ATCCAGCATA GTGGATTTGT GAAACAACTG 
CTGCTTTATT TGACATCTAA AAGTGAGAAA GATGCTGTAA GCAGGGATAT CAGATTGAAA 
AGATTTCTTC ATGTATTTTT CTCCTCTCCA CTTCCTGGAG AAGAACCCCT TGGAAGATTA 
GAGCCATTAG AAAATGCACC TTTGTTGGCA TTAGTCCATA AAATGAACAA TTGCCTCAGT 
CAGATGGAGC AGTTTCCAGT GAAAGTGCAT GACTTCCCCA GTGGAAATGG AACAGGGAGC 
AGTTTTTCTC TTAACAGAGG ATCCCAAGCT TTAAAATTCT TCAATACGCA TCAGTTAAAA 
TGCCAGCTGC AAAGACATCC AGACTGTGCT AATGTGAAAC AGTGGAAGGG TGGACCTGTG 
AAGATTGATC CTCTGGCTTT GGTTCAAGCC ATTGAAAGAT ATCTTGTTGT TAGAGGTTAT 
GGAAGAGTTA GAGAAGATGA TGAGGATAGT GATGATGATG GGTCAGATGA AGAAATAGAT 
GAATCCTTGG CTGCTCAATT CTTAAATTCA GGGAATGTGA GACATAGATT GCAGTTTTAC 
ATTGGGGATC ACTTGCTACC ATATAATATG ACTGTATATC AAGCAGTTAA GCTGTACAGT 
TTGCAAGCTG AGGAGGAGAG GGAATCGACT GATGATGAAA GCAATCCATT AGGAAGAGCT 
GGGATTTGGA CAAAAACACA TACCATTTGG TACAAACCTG TACGGGAGGA TGAAGATGGC 
AACAAAGACT GTGTTGGTGG TAAAAGAGGA AGAGCTCAAA CTGCTCCCAC AAAAACCTCA 
CCTAGGAATT CTAAAAAGCA TGATGAGTTG TGGCACGATG GTGTATGCCC TTCAGTATTG 
AATCCTTTAG AAGTTTACCT CATATCTACT CCTCCTGAAA ACATAACCTT TGAAGATCCT 
TCATTAGATG TTATTCTTCT TTTGAGAGTT TTACATGCTA TCAGTCGATA CTGGTATTAT 
TTGTATGATA ATGCAATATG CAAGGAGATT ATTCCAACTT CGGAATTTAT CAATAGTAAA 
CTGACAGCAA AAGCAAACAG ACAGCTTCAA GATCCACTGG TAATTATGAC AGGAAACATA 
CCGACTTGGC TGACAGAGCT TGGAAAAACA TGCCCATTTT TCTTTCCATT TGATACCCGG 
CAAATGCTGT TTTATGTAAC TGCTTTTGAT CGTGATCGAG CCATGCAAAG ACTACTGGAT 
ACTAATCCAG AAATCAATCA ATCAGATTCT CAGGATAGCA GAGTGGCACC ACGACTGGAC 
AGGAAAAAAC GCACTGTGAA CAGAGATGAG CTGTTGAAAC AGGCAGAATC TGTGATGCAG 
GATCTAGGCA GTTCAAGAGC CATGTTGGAA ATCCAGTATG AGAATGAAGT TGGCACAGGC 
CTGGGCCCTA CACTAGAGTT CTATGCACTT GTATCTCAGG AACTACAGAG AGCAGACTTG 
GGCCTTTGGA GGGGAGAAGA AGTAACTTTA GCCAATCCAA AAGGAAGCCA AGAAGGTACC 
AAGTATATTC ATAATCTCCA AGGCCTTTTT GCACTTCCTT TTGGTAGAAC AGCCAAGCCT 
GCTCACATTG CAAAAGTTAA AATGAAGTTC CGCTTTCTGG GAAAACTAAT GGCTAAGGCA 
ATCATGGATT TTAGACTGGT GGATCTTCCT CTTGGACTTC CTTTTTATAA ATGGATGTTA 
CGCCAGGAAA CTTCTTTGAC GTCACATGAC TTGTTCAGTA TTGATCCAGT AGTAGCCAAA 
TCAATATATC ACCTTGAAGA TATTGTAAGA CAAAAGAAAA GACTTGAGCA GGACAAAACA 
CAGACCAAAG AAAGTCTACA GTATGCATTG GAAACCCTGA CTATGAATGG CTGCTCAGTG 
GAGGATCTAG GGCTGGATTT CACACTTCCT GGATTTCCTA ACATCGAGCT GAAAAAAGGG 
GGAAAAGATA CACCAGTCAC CATCCACAAC TTGGAGGAGT ACCTCAGATT GGTTATATTC 
TGGGCACTTA ACGAAGGTGT TGCCAGACAG TTTGATTCTT TCAGAGATGG ATTTGAATCA 
GTCTTCCCCC TCAGTCATCT TCAATACTTT TATCCTGAGG AGTTGGAGCA ACTCTTATGT 
GGCAGCAAAA CAGACACATG GGATGCCAAG ACCTTAATGG AATGTTGCAG GCCAGATCAC 
GGTTATACAC ATGACAGTCG AGCAGTGAAG TATCTCTTTG AAATTCTCAG TAGTTTTGAT 
AGTGAGCAGC AGAGACTGTT TCTTCAGTTT GTGACCGGTA GTCCCAGGCT GCCAGTAGGA 
GGCTTTCGAA GTTTGAATCC TCCATTGACA ATTGTACGGA AGACATTTGA GTCTACAGAG 
AATCCAGATG ACTTCTTGCC CTCAGTAATG ACTTGTGTGA ACTATCTCAA ATTGCCGGAC 
TATTCAACTA TTGAGATAAT GCGTGAAAAA CTCTTGATAG CTGCAAGAGA AGGGCAGCAG 
TCATTCCATC TTTCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TRIP12 ( XM_015276964.2 ) cDNA ORF clone, Gallus gallus(chicken) -> XP_015132450.1 Gallus gallus thyroid hormone receptor interactor 12 (TRIP12), transcript variant X2, mRNA.

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CloneID	OGa12502
Clone ID Related Accession (Same CDS sequence)	XM_015276964.1 , XM_015276968.2 , XM_015276967.1 , XM_015276968.1 , XM_004937064.3 , XM_015276964.2 , XM_015276966.2 , XM_004937064.2 , XM_015276966.1 , XM_015276967.2 , XM_015276965.1
Accession Version	XM_015276964.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6198bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-16
Organism	Gallus gallus(chicken)
Product	E3 ubiquitin-protein ligase TRIP12 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006096.5) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On May 17, 2018 this sequence version replaced XM_015276964.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Gallus gallus Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCCAACC GGCCTAATAA CAATCCAGGG GGGTCACTGC GACGTTCACA GAGGAACACT 
GCTGGGGCCC AGCCACAAGA CGACGCAGCA GGAGGAAGGT CACATTTAGG GCAGGCAAAA 
CATAAGGCAC ATAGCCCTCC TGAGAGTAGA AAATCTATTT CAAAGACACC CAAAGTGCAG 
TCTAATACTA CTTCTGAGCA GTCCAAGGGA CACTTTTCTA AAAGAGGCTG TAGTTCATCT 
GCTGTTTTAA TACCACAAGA AGATTCAGAA AGAGTCAGTA CTTCAGAAAA GCAAAAAACG 
GGGCAAGTGC CTAAGAAAGA CAATTCTCGA GGAGTTAAAC GCAGTGCTAG TCCAGATTAC 
AGCAGGACCA ATTCTCCTAG CTCTGCTAAA AAACCTAAAG CACTTCAACA CGCCGAAACT 
TCCTCGGAAA GTAACAAGCC ACATACTAAA TCCAAGAGAA GACACTTAGA CCAAGAACAG 
CAGAAGTCTA CACAATTGCC ATCAACAAGC AAGGCTCACA CCAGAAAGGG TGGAGCTGCT 
GGTAGCTCCC GAAGTCAGAA AAGGAAAAGG ACAGAGAGTA CGTCTTGTAT AAAGAGTCGT 
TCAGCAGTTG AATCAACTGG AGCTGAAGAG AAGTCAGCAA AATCCTCCAA GCTGGCTTCA 
AAATCGGTGA CCTCAGCCAA AGCTGGGTGT AGCACCATCA CTGATTCTTC TTCTTCAGCT 
TCTACATCCT CTTCCTCTTC TGCTGTTGCC TCTGCTTCTT CCACTGTTCC TCAGGGTGCC 
AGAGTGAAAC AGGGTAAGGA CCAGAATAAG GCTAGACGTT CACGTTCTGC GTCCAGCCCT 
AGTCCAAGAA GGAGCAGCAG GGACAAAGAA CAGAGCAAAA CAGGTGGCTC TTCAAAGTTT 
GACTGGGCTG CTCGATTCAG TCCAAAAGTT AGTCTCCCTA AAACAAAACT GTCTCTACCA 
GGCTCTTCTA AGTCAGAGAC CTCAAAACCT GGACCTTCAG GACTACAGGC TAAGCTAGCA 
AGCCTAAGAA AATCCACAAA GAAGCGCAGT GAATCACCAC CTGCTGAGCT CCCCAGTTTA 
CGGCGGAGCA CACGGCAAAA GACCACGGGC TCCTGTGCGA GTACCAGTCG GCGGGGCTCT 
GGCCTGGGCA AAAGAGGAGC AGCTGAAGCT CGCCGACAGG AGAAGATGGC TGATCCTGAC 
AACAACCAGG ATGGAGTTAA CTCTTCAGCT GCACGTACAG ATGAGACTCC CCAGGGAGCT 
GCAGCTTCTA GCTCTGTTGC TGGGGCTGTA GGTATGACAA CATCTGGAGA AAGTGAGTCA 
GATGACTCTG AGATGGGAAG ACTACAAGCT CTGTTAGAGG CTAGGGGTCT TCCTCCTCAC 
CTGTTTGGCC CTCTTGGTCC TCGGATGTCG CAGCTCTTCC ACAGGACAAT TGGAAGTGGA 
GCTAGTTCTA AAGCCCAGCA GCTTTTACAA GGTCTCCAAG CCACTGATGA AAGTCAGCAA 
CTACAGGCAG TTATTGAGAT GTGCCAGCTG TTGGTCATGG GAAATGAAGA AACACTGGGA 
GGATTTCCAG TCAAGAGTGT TGTGCCAGCT TTGATAACAT TGCTGCAGAT GGAGCATAAC 
TTTGACATCA TGAACCATGC TTGTCGAGCC TTAACATACA TGATGGAAGC ACTTCCCAGA 
TCATCTGCTG TAGTGGTAGA TGCAATTCCT GTCTTCTTGG AAAAGCTGCA AGTTATTCAG 
TGCATTGATG TGGCAGAGCA GGCACTGACA GCCCTAGAGA TGCTATCACG CAGGCATAGT 
AAAGCCATTC TGCAGGCAGG TGGGTTGGCA GACTGCTTGC TGTATCTGGA ATTCTTCAGT 
ATAAATGCGC AGAGGAATGC ACTAGCTATT GCTGCCAATT GCTGCCAGAG TATAACACCT 
GATGAGTTTC ACTTTGTGGC AGACTCTTTG CCACTGCTTA CACAAAGGTT AACCCATCAG 
GACAAGAAGT CTGTTGAAAG CACTTGTCTC TGTTTTGCAC GGCTGGTAGA CAATTTCCAG 
CATGAGGAGA ACTTGCTGCA GCAGGTTGCT TCCAAGGACT TGTTAACAAA CATCCAGCAA 
CTCTTGGTAG TGACACCTCC TATCCTGAGC TCAGGAATGT TCATCATGGT GGTGCGCATG 
TTTTCCTTAA TGTGTTCCAA TTGTCCAACG CTTGCAGTCC AACTTATGAA GCAAAACATT 
GCAGAAACAC TTCACTTTCT CCTTTGTGGA GCCTCAAATG GGAGTTGTCA AGAACAAATT 
GACCTTGTTC CACGAAGTCC TCAAGAACTC TATGAGCTTA CTTCTCTTAT CTGTGAACTG 
ATGCCTTGTC TGCCAAAAGA GGGGATATTT GCTGTTGATA CTATGCTAAA GAAAGGAAAT 
GCACAAAATA CAGATGGTGC GATATGGCAA TGGCGAGATG ACAGGGGTCT CTGGCATCCC 
TATAACAGGA TTGATAGTCG AATAATAGAG GCAGCTCATC AGGTTGGTGA GGATGAGATA 
AGCCTGTCGA CACTTGGACG TGTCTATACT ATTGATTTTA ATTCTATGCA GCAAATAAAT 
GAGGATACTG GAACAGCACG TGCCATTCAG CGGAAACCAA ACCCTTTAGC CAATACAAAC 
ACTAGTGGAC ATTCAGAATT GAAGAAGGAT GATGCTCGAG CACAGCTAAT GAAAGAAGAC 
CCAGAATTGG CAAAATCCTT TATCAAAACA CTGTTTGGTG TTCTTTATGA AGTGTATAGT 
TCTTCAGCTG GACCTGCTGT TAGACACAAG TGCCTTAGAG CAATACTTAG AATAATTTAT 
TTTGCTGATG CTGAACTCCT GAAGGATGTG CTGAAAAACC ATGCTGTGTC AAGTCACATT 
GCCTCCATGC TGTCAAGTCA AGACCTTAAG ATAGTAGTTG GAGCCCTGCA GATGGCAGAA 
ATCTTAATGC AGAAGCTACC TGACATTTTT AGTGTTTACT TCAGAAGAGA AGGAGTGATG 
CATCAAGTGA AAAACTTAGC GGAGTCTGAG GCTTTGCTAA CGAGCCCACC AAAAGTATGC 
ACTAATGGAT CAGGAACACT GGGTACCACT ACAACTATAA GTACTGGAAC AGCCACTGCT 
GCCACTAATG CAGCTGCAGA TTTGGGCTCT CCCAGCTTAC AACACAGCCG GGAGGATTCT 
TTAGATCTGA GCCCACAGGG ACGACTGAGT GATGTTCTGA AGAGAAAAAG ACTGCCAAAA 
CGAGGGCCAA GGAGACCAAA ATATTCTCCC CCAAGAGATG ATGACAAAGT AGACAATCAA 
GCAAAAAGCC CCACGACTAC TCAGTCTCCT AAATCTTCTT TCTTAGCAAG TTTAAATCCC 
AAAACATGGG GAAGATTAAG TACACAGTCT AACAGTAATA ACATTGAACC AGCAAGAACA 
GCAGGTGTAA GTGGTCTTGC AAGAGCTGCT TCTAAGGATA CAATTTCCAA TAACAGAGAA 
AAAATTAAGG GTTGGATTAA GGAGCAAGCC CATAAATTTG TAGACCGTTA TTTTAGTTCG 
GAAAACATGG ATGGAAGCAA TCCTGCACTA AATGTATTAC AGAGACTTTG CACTGCAACT 
GAACAACTTA ACCTCCAGGT GGATGGTGGA ACAGAGTGCC TTGTAGAAAT CCGTAGCATT 
GTCTCGGAGT CTGACGTCTC CTCATTTGAA ATCCAGCATA GTGGATTTGT GAAACAACTG 
CTGCTTTATT TGACATCTAA AAGTGAGAAA GATGCTGTAA GCAGGGATAT CAGATTGAAA 
AGATTTCTTC ATGTATTTTT CTCCTCTCCA CTTCCTGGAG AAGAACCCCT TGGAAGATTA 
GAGCCATTAG AAAATGCACC TTTGTTGGCA TTAGTCCATA AAATGAACAA TTGCCTCAGT 
CAGATGGAGC AGTTTCCAGT GAAAGTGCAT GACTTCCCCA GTGGAAATGG AACAGGGAGC 
AGTTTTTCTC TTAACAGAGG ATCCCAAGCT TTAAAATTCT TCAATACGCA TCAGTTAAAA 
TGCCAGCTGC AAAGACATCC AGACTGTGCT AATGTGAAAC AGTGGAAGGG TGGACCTGTG 
AAGATTGATC CTCTGGCTTT GGTTCAAGCC ATTGAAAGAT ATCTTGTTGT TAGAGGTTAT 
GGAAGAGTTA GAGAAGATGA TGAGGATAGT GATGATGATG GGTCAGATGA AGAAATAGAT 
GAATCCTTGG CTGCTCAATT CTTAAATTCA GGGAATGTGA GACATAGATT GCAGTTTTAC 
ATTGGGGATC ACTTGCTACC ATATAATATG ACTGTATATC AAGCAGTTAA GCTGTACAGT 
TTGCAAGCTG AGGAGGAGAG GGAATCGACT GATGATGAAA GCAATCCATT AGGAAGAGCT 
GGGATTTGGA CAAAAACACA TACCATTTGG TACAAACCTG TACGGGAGGA TGAAGATGGC 
AACAAAGACT GTGTTGGTGG TAAAAGAGGA AGAGCTCAAA CTGCTCCCAC AAAAACCTCA 
CCTAGGAATT CTAAAAAGCA TGATGAGTTG TGGCACGATG GTGTATGCCC TTCAGTATTG 
AATCCTTTAG AAGTTTACCT CATATCTACT CCTCCTGAAA ACATAACCTT TGAAGATCCT 
TCATTAGATG TTATTCTTCT TTTGAGAGTT TTACATGCTA TCAGTCGATA CTGGTATTAT 
TTGTATGATA ATGCAATATG CAAGGAGATT ATTCCAACTT CGGAATTTAT CAATAGTAAA 
CTGACAGCAA AAGCAAACAG ACAGCTTCAA GATCCACTGG TAATTATGAC AGGAAACATA 
CCGACTTGGC TGACAGAGCT TGGAAAAACA TGCCCATTTT TCTTTCCATT TGATACCCGG 
CAAATGCTGT TTTATGTAAC TGCTTTTGAT CGTGATCGAG CCATGCAAAG ACTACTGGAT 
ACTAATCCAG AAATCAATCA ATCAGATTCT CAGGATAGCA GAGTGGCACC ACGACTGGAC 
AGGAAAAAAC GCACTGTGAA CAGAGATGAG CTGTTGAAAC AGGCAGAATC TGTGATGCAG 
GATCTAGGCA GTTCAAGAGC CATGTTGGAA ATCCAGTATG AGAATGAAGT TGGCACAGGC 
CTGGGCCCTA CACTAGAGTT CTATGCACTT GTATCTCAGG AACTACAGAG AGCAGACTTG 
GGCCTTTGGA GGGGAGAAGA AGTAACTTTA GCCAATCCAA AAGGAAGCCA AGAAGGTACC 
AAGTATATTC ATAATCTCCA AGGCCTTTTT GCACTTCCTT TTGGTAGAAC AGCCAAGCCT 
GCTCACATTG CAAAAGTTAA AATGAAGTTC CGCTTTCTGG GAAAACTAAT GGCTAAGGCA 
ATCATGGATT TTAGACTGGT GGATCTTCCT CTTGGACTTC CTTTTTATAA ATGGATGTTA 
CGCCAGGAAA CTTCTTTGAC GTCACATGAC TTGTTCAGTA TTGATCCAGT AGTAGCCAAA 
TCAATATATC ACCTTGAAGA TATTGTAAGA CAAAAGAAAA GACTTGAGCA GGACAAAACA 
CAGACCAAAG AAAGTCTACA GTATGCATTG GAAACCCTGA CTATGAATGG CTGCTCAGTG 
GAGGATCTAG GGCTGGATTT CACACTTCCT GGATTTCCTA ACATCGAGCT GAAAAAAGGG 
GGAAAAGATA CACCAGTCAC CATCCACAAC TTGGAGGAGT ACCTCAGATT GGTTATATTC 
TGGGCACTTA ACGAAGGTGT TGCCAGACAG TTTGATTCTT TCAGAGATGG ATTTGAATCA 
GTCTTCCCCC TCAGTCATCT TCAATACTTT TATCCTGAGG AGTTGGAGCA ACTCTTATGT 
GGCAGCAAAA CAGACACATG GGATGCCAAG ACCTTAATGG AATGTTGCAG GCCAGATCAC 
GGTTATACAC ATGACAGTCG AGCAGTGAAG TATCTCTTTG AAATTCTCAG TAGTTTTGAT 
AGTGAGCAGC AGAGACTGTT TCTTCAGTTT GTGACCGGTA GTCCCAGGCT GCCAGTAGGA 
GGCTTTCGAA GTTTGAATCC TCCATTGACA ATTGTACGGA AGACATTTGA GTCTACAGAG 
AATCCAGATG ACTTCTTGCC CTCAGTAATG ACTTGTGTGA ACTATCTCAA ATTGCCGGAC 
TATTCAACTA TTGAGATAAT GCGTGAAAAA CTCTTGATAG CTGCAAGAGA AGGGCAGCAG 
TCATTCCATC TTTCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015132450.1
CDS	251..6448
Translation	MSNRPNNNPGGSLRRSQRNTAGAQPQDDAAGGRSHLGQAKHKAHSPPESRKSISKTPKVQSNTTSEQSKGHFSKRGCSSSAVLIPQEDSERVSTSEKQKTGQVPKKDNSRGVKRSASPDYSRTNSPSSAKKPKALQHAETSSESNKPHTKSKRRHLDQEQQKSTQLPSTSKAHTRKGGAAGSSRSQKRKRTESTSCIKSRSAVESTGAEEKSAKSSKLASKSVTSAKAGCSTITDSSSSASTSSSSSAVASASSTVPQGARVKQGKDQNKARRSRSASSPSPRRSSRDKEQSKTGGSSKFDWAARFSPKVSLPKTKLSLPGSSKSETSKPGPSGLQAKLASLRKSTKKRSESPPAELPSLRRSTRQKTTGSCASTSRRGSGLGKRGAAEARRQEKMADPDNNQDGVNSSAARTDETPQGAAASSSVAGAVGMTTSGESESDDSEMGRLQALLEARGLPPHLFGPLGPRMSQLFHRTIGSGASSKAQQLLQGLQATDESQQLQAVIEMCQLLVMGNEETLGGFPVKSVVPALITLLQMEHNFDIMNHACRALTYMMEALPRSSAVVVDAIPVFLEKLQVIQCIDVAEQALTALEMLSRRHSKAILQAGGLADCLLYLEFFSINAQRNALAIAANCCQSITPDEFHFVADSLPLLTQRLTHQDKKSVESTCLCFARLVDNFQHEENLLQQVASKDLLTNIQQLLVVTPPILSSGMFIMVVRMFSLMCSNCPTLAVQLMKQNIAETLHFLLCGASNGSCQEQIDLVPRSPQELYELTSLICELMPCLPKEGIFAVDTMLKKGNAQNTDGAIWQWRDDRGLWHPYNRIDSRIIEAAHQVGEDEISLSTLGRVYTIDFNSMQQINEDTGTARAIQRKPNPLANTNTSGHSELKKDDARAQLMKEDPELAKSFIKTLFGVLYEVYSSSAGPAVRHKCLRAILRIIYFADAELLKDVLKNHAVSSHIASMLSSQDLKIVVGALQMAEILMQKLPDIFSVYFRREGVMHQVKNLAESEALLTSPPKVCTNGSGTLGTTTTISTGTATAATNAAADLGSPSLQHSREDSLDLSPQGRLSDVLKRKRLPKRGPRRPKYSPPRDDDKVDNQAKSPTTTQSPKSSFLASLNPKTWGRLSTQSNSNNIEPARTAGVSGLARAASKDTISNNREKIKGWIKEQAHKFVDRYFSSENMDGSNPALNVLQRLCTATEQLNLQVDGGTECLVEIRSIVSESDVSSFEIQHSGFVKQLLLYLTSKSEKDAVSRDIRLKRFLHVFFSSPLPGEEPLGRLEPLENAPLLALVHKMNNCLSQMEQFPVKVHDFPSGNGTGSSFSLNRGSQALKFFNTHQLKCQLQRHPDCANVKQWKGGPVKIDPLALVQAIERYLVVRGYGRVREDDEDSDDDGSDEEIDESLAAQFLNSGNVRHRLQFYIGDHLLPYNMTVYQAVKLYSLQAEEERESTDDESNPLGRAGIWTKTHTIWYKPVREDEDGNKDCVGGKRGRAQTAPTKTSPRNSKKHDELWHDGVCPSVLNPLEVYLISTPPENITFEDPSLDVILLLRVLHAISRYWYYLYDNAICKEIIPTSEFINSKLTAKANRQLQDPLVIMTGNIPTWLTELGKTCPFFFPFDTRQMLFYVTAFDRDRAMQRLLDTNPEINQSDSQDSRVAPRLDRKKRTVNRDELLKQAESVMQDLGSSRAMLEIQYENEVGTGLGPTLEFYALVSQELQRADLGLWRGEEVTLANPKGSQEGTKYIHNLQGLFALPFGRTAKPAHIAKVKMKFRFLGKLMAKAIMDFRLVDLPLGLPFYKWMLRQETSLTSHDLFSIDPVVAKSIYHLEDIVRQKKRLEQDKTQTKESLQYALETLTMNGCSVEDLGLDFTLPGFPNIELKKGGKDTPVTIHNLEEYLRLVIFWALNEGVARQFDSFRDGFESVFPLSHLQYFYPEELEQLLCGSKTDTWDAKTLMECCRPDHGYTHDSRAVKYLFEILSSFDSEQQRLFLQFVTGSPRLPVGGFRSLNPPLTIVRKTFESTENPDDFLPSVMTCVNYLKLPDYSTIEIMREKLLIAAREGQQSFHLS

Target ORF information:

RefSeq Version	XM_015276964.2
Organism	Gallus gallus(chicken)
Definition	Gallus gallus thyroid hormone receptor interactor 12 (TRIP12), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015276964.2

ORF Insert Sequence:

ATGTCCAACC GGCCTAATAA CAATCCAGGG GGGTCACTGC GACGTTCACA GAGGAACACT 
GCTGGGGCCC AGCCACAAGA CGACGCAGCA GGAGGAAGGT CACATTTAGG GCAGGCAAAA 
CATAAGGCAC ATAGCCCTCC TGAGAGTAGA AAATCTATTT CAAAGACACC CAAAGTGCAG 
TCTAATACTA CTTCTGAGCA GTCCAAGGGA CACTTTTCTA AAAGAGGCTG TAGTTCATCT 
GCTGTTTTAA TACCACAAGA AGATTCAGAA AGAGTCAGTA CTTCAGAAAA GCAAAAAACG 
GGGCAAGTGC CTAAGAAAGA CAATTCTCGA GGAGTTAAAC GCAGTGCTAG TCCAGATTAC 
AGCAGGACCA ATTCTCCTAG CTCTGCTAAA AAACCTAAAG CACTTCAACA CGCCGAAACT 
TCCTCGGAAA GTAACAAGCC ACATACTAAA TCCAAGAGAA GACACTTAGA CCAAGAACAG 
CAGAAGTCTA CACAATTGCC ATCAACAAGC AAGGCTCACA CCAGAAAGGG TGGAGCTGCT 
GGTAGCTCCC GAAGTCAGAA AAGGAAAAGG ACAGAGAGTA CGTCTTGTAT AAAGAGTCGT 
TCAGCAGTTG AATCAACTGG AGCTGAAGAG AAGTCAGCAA AATCCTCCAA GCTGGCTTCA 
AAATCGGTGA CCTCAGCCAA AGCTGGGTGT AGCACCATCA CTGATTCTTC TTCTTCAGCT 
TCTACATCCT CTTCCTCTTC TGCTGTTGCC TCTGCTTCTT CCACTGTTCC TCAGGGTGCC 
AGAGTGAAAC AGGGTAAGGA CCAGAATAAG GCTAGACGTT CACGTTCTGC GTCCAGCCCT 
AGTCCAAGAA GGAGCAGCAG GGACAAAGAA CAGAGCAAAA CAGGTGGCTC TTCAAAGTTT 
GACTGGGCTG CTCGATTCAG TCCAAAAGTT AGTCTCCCTA AAACAAAACT GTCTCTACCA 
GGCTCTTCTA AGTCAGAGAC CTCAAAACCT GGACCTTCAG GACTACAGGC TAAGCTAGCA 
AGCCTAAGAA AATCCACAAA GAAGCGCAGT GAATCACCAC CTGCTGAGCT CCCCAGTTTA 
CGGCGGAGCA CACGGCAAAA GACCACGGGC TCCTGTGCGA GTACCAGTCG GCGGGGCTCT 
GGCCTGGGCA AAAGAGGAGC AGCTGAAGCT CGCCGACAGG AGAAGATGGC TGATCCTGAC 
AACAACCAGG ATGGAGTTAA CTCTTCAGCT GCACGTACAG ATGAGACTCC CCAGGGAGCT 
GCAGCTTCTA GCTCTGTTGC TGGGGCTGTA GGTATGACAA CATCTGGAGA AAGTGAGTCA 
GATGACTCTG AGATGGGAAG ACTACAAGCT CTGTTAGAGG CTAGGGGTCT TCCTCCTCAC 
CTGTTTGGCC CTCTTGGTCC TCGGATGTCG CAGCTCTTCC ACAGGACAAT TGGAAGTGGA 
GCTAGTTCTA AAGCCCAGCA GCTTTTACAA GGTCTCCAAG CCACTGATGA AAGTCAGCAA 
CTACAGGCAG TTATTGAGAT GTGCCAGCTG TTGGTCATGG GAAATGAAGA AACACTGGGA 
GGATTTCCAG TCAAGAGTGT TGTGCCAGCT TTGATAACAT TGCTGCAGAT GGAGCATAAC 
TTTGACATCA TGAACCATGC TTGTCGAGCC TTAACATACA TGATGGAAGC ACTTCCCAGA 
TCATCTGCTG TAGTGGTAGA TGCAATTCCT GTCTTCTTGG AAAAGCTGCA AGTTATTCAG 
TGCATTGATG TGGCAGAGCA GGCACTGACA GCCCTAGAGA TGCTATCACG CAGGCATAGT 
AAAGCCATTC TGCAGGCAGG TGGGTTGGCA GACTGCTTGC TGTATCTGGA ATTCTTCAGT 
ATAAATGCGC AGAGGAATGC ACTAGCTATT GCTGCCAATT GCTGCCAGAG TATAACACCT 
GATGAGTTTC ACTTTGTGGC AGACTCTTTG CCACTGCTTA CACAAAGGTT AACCCATCAG 
GACAAGAAGT CTGTTGAAAG CACTTGTCTC TGTTTTGCAC GGCTGGTAGA CAATTTCCAG 
CATGAGGAGA ACTTGCTGCA GCAGGTTGCT TCCAAGGACT TGTTAACAAA CATCCAGCAA 
CTCTTGGTAG TGACACCTCC TATCCTGAGC TCAGGAATGT TCATCATGGT GGTGCGCATG 
TTTTCCTTAA TGTGTTCCAA TTGTCCAACG CTTGCAGTCC AACTTATGAA GCAAAACATT 
GCAGAAACAC TTCACTTTCT CCTTTGTGGA GCCTCAAATG GGAGTTGTCA AGAACAAATT 
GACCTTGTTC CACGAAGTCC TCAAGAACTC TATGAGCTTA CTTCTCTTAT CTGTGAACTG 
ATGCCTTGTC TGCCAAAAGA GGGGATATTT GCTGTTGATA CTATGCTAAA GAAAGGAAAT 
GCACAAAATA CAGATGGTGC GATATGGCAA TGGCGAGATG ACAGGGGTCT CTGGCATCCC 
TATAACAGGA TTGATAGTCG AATAATAGAG GCAGCTCATC AGGTTGGTGA GGATGAGATA 
AGCCTGTCGA CACTTGGACG TGTCTATACT ATTGATTTTA ATTCTATGCA GCAAATAAAT 
GAGGATACTG GAACAGCACG TGCCATTCAG CGGAAACCAA ACCCTTTAGC CAATACAAAC 
ACTAGTGGAC ATTCAGAATT GAAGAAGGAT GATGCTCGAG CACAGCTAAT GAAAGAAGAC 
CCAGAATTGG CAAAATCCTT TATCAAAACA CTGTTTGGTG TTCTTTATGA AGTGTATAGT 
TCTTCAGCTG GACCTGCTGT TAGACACAAG TGCCTTAGAG CAATACTTAG AATAATTTAT 
TTTGCTGATG CTGAACTCCT GAAGGATGTG CTGAAAAACC ATGCTGTGTC AAGTCACATT 
GCCTCCATGC TGTCAAGTCA AGACCTTAAG ATAGTAGTTG GAGCCCTGCA GATGGCAGAA 
ATCTTAATGC AGAAGCTACC TGACATTTTT AGTGTTTACT TCAGAAGAGA AGGAGTGATG 
CATCAAGTGA AAAACTTAGC GGAGTCTGAG GCTTTGCTAA CGAGCCCACC AAAAGTATGC 
ACTAATGGAT CAGGAACACT GGGTACCACT ACAACTATAA GTACTGGAAC AGCCACTGCT 
GCCACTAATG CAGCTGCAGA TTTGGGCTCT CCCAGCTTAC AACACAGCCG GGAGGATTCT 
TTAGATCTGA GCCCACAGGG ACGACTGAGT GATGTTCTGA AGAGAAAAAG ACTGCCAAAA 
CGAGGGCCAA GGAGACCAAA ATATTCTCCC CCAAGAGATG ATGACAAAGT AGACAATCAA 
GCAAAAAGCC CCACGACTAC TCAGTCTCCT AAATCTTCTT TCTTAGCAAG TTTAAATCCC 
AAAACATGGG GAAGATTAAG TACACAGTCT AACAGTAATA ACATTGAACC AGCAAGAACA 
GCAGGTGTAA GTGGTCTTGC AAGAGCTGCT TCTAAGGATA CAATTTCCAA TAACAGAGAA 
AAAATTAAGG GTTGGATTAA GGAGCAAGCC CATAAATTTG TAGACCGTTA TTTTAGTTCG 
GAAAACATGG ATGGAAGCAA TCCTGCACTA AATGTATTAC AGAGACTTTG CACTGCAACT 
GAACAACTTA ACCTCCAGGT GGATGGTGGA ACAGAGTGCC TTGTAGAAAT CCGTAGCATT 
GTCTCGGAGT CTGACGTCTC CTCATTTGAA ATCCAGCATA GTGGATTTGT GAAACAACTG 
CTGCTTTATT TGACATCTAA AAGTGAGAAA GATGCTGTAA GCAGGGATAT CAGATTGAAA 
AGATTTCTTC ATGTATTTTT CTCCTCTCCA CTTCCTGGAG AAGAACCCCT TGGAAGATTA 
GAGCCATTAG AAAATGCACC TTTGTTGGCA TTAGTCCATA AAATGAACAA TTGCCTCAGT 
CAGATGGAGC AGTTTCCAGT GAAAGTGCAT GACTTCCCCA GTGGAAATGG AACAGGGAGC 
AGTTTTTCTC TTAACAGAGG ATCCCAAGCT TTAAAATTCT TCAATACGCA TCAGTTAAAA 
TGCCAGCTGC AAAGACATCC AGACTGTGCT AATGTGAAAC AGTGGAAGGG TGGACCTGTG 
AAGATTGATC CTCTGGCTTT GGTTCAAGCC ATTGAAAGAT ATCTTGTTGT TAGAGGTTAT 
GGAAGAGTTA GAGAAGATGA TGAGGATAGT GATGATGATG GGTCAGATGA AGAAATAGAT 
GAATCCTTGG CTGCTCAATT CTTAAATTCA GGGAATGTGA GACATAGATT GCAGTTTTAC 
ATTGGGGATC ACTTGCTACC ATATAATATG ACTGTATATC AAGCAGTTAA GCTGTACAGT 
TTGCAAGCTG AGGAGGAGAG GGAATCGACT GATGATGAAA GCAATCCATT AGGAAGAGCT 
GGGATTTGGA CAAAAACACA TACCATTTGG TACAAACCTG TACGGGAGGA TGAAGATGGC 
AACAAAGACT GTGTTGGTGG TAAAAGAGGA AGAGCTCAAA CTGCTCCCAC AAAAACCTCA 
CCTAGGAATT CTAAAAAGCA TGATGAGTTG TGGCACGATG GTGTATGCCC TTCAGTATTG 
AATCCTTTAG AAGTTTACCT CATATCTACT CCTCCTGAAA ACATAACCTT TGAAGATCCT 
TCATTAGATG TTATTCTTCT TTTGAGAGTT TTACATGCTA TCAGTCGATA CTGGTATTAT 
TTGTATGATA ATGCAATATG CAAGGAGATT ATTCCAACTT CGGAATTTAT CAATAGTAAA 
CTGACAGCAA AAGCAAACAG ACAGCTTCAA GATCCACTGG TAATTATGAC AGGAAACATA 
CCGACTTGGC TGACAGAGCT TGGAAAAACA TGCCCATTTT TCTTTCCATT TGATACCCGG 
CAAATGCTGT TTTATGTAAC TGCTTTTGAT CGTGATCGAG CCATGCAAAG ACTACTGGAT 
ACTAATCCAG AAATCAATCA ATCAGATTCT CAGGATAGCA GAGTGGCACC ACGACTGGAC 
AGGAAAAAAC GCACTGTGAA CAGAGATGAG CTGTTGAAAC AGGCAGAATC TGTGATGCAG 
GATCTAGGCA GTTCAAGAGC CATGTTGGAA ATCCAGTATG AGAATGAAGT TGGCACAGGC 
CTGGGCCCTA CACTAGAGTT CTATGCACTT GTATCTCAGG AACTACAGAG AGCAGACTTG 
GGCCTTTGGA GGGGAGAAGA AGTAACTTTA GCCAATCCAA AAGGAAGCCA AGAAGGTACC 
AAGTATATTC ATAATCTCCA AGGCCTTTTT GCACTTCCTT TTGGTAGAAC AGCCAAGCCT 
GCTCACATTG CAAAAGTTAA AATGAAGTTC CGCTTTCTGG GAAAACTAAT GGCTAAGGCA 
ATCATGGATT TTAGACTGGT GGATCTTCCT CTTGGACTTC CTTTTTATAA ATGGATGTTA 
CGCCAGGAAA CTTCTTTGAC GTCACATGAC TTGTTCAGTA TTGATCCAGT AGTAGCCAAA 
TCAATATATC ACCTTGAAGA TATTGTAAGA CAAAAGAAAA GACTTGAGCA GGACAAAACA 
CAGACCAAAG AAAGTCTACA GTATGCATTG GAAACCCTGA CTATGAATGG CTGCTCAGTG 
GAGGATCTAG GGCTGGATTT CACACTTCCT GGATTTCCTA ACATCGAGCT GAAAAAAGGG 
GGAAAAGATA CACCAGTCAC CATCCACAAC TTGGAGGAGT ACCTCAGATT GGTTATATTC 
TGGGCACTTA ACGAAGGTGT TGCCAGACAG TTTGATTCTT TCAGAGATGG ATTTGAATCA 
GTCTTCCCCC TCAGTCATCT TCAATACTTT TATCCTGAGG AGTTGGAGCA ACTCTTATGT 
GGCAGCAAAA CAGACACATG GGATGCCAAG ACCTTAATGG AATGTTGCAG GCCAGATCAC 
GGTTATACAC ATGACAGTCG AGCAGTGAAG TATCTCTTTG AAATTCTCAG TAGTTTTGAT 
AGTGAGCAGC AGAGACTGTT TCTTCAGTTT GTGACCGGTA GTCCCAGGCT GCCAGTAGGA 
GGCTTTCGAA GTTTGAATCC TCCATTGACA ATTGTACGGA AGACATTTGA GTCTACAGAG 
AATCCAGATG ACTTCTTGCC CTCAGTAATG ACTTGTGTGA ACTATCTCAA ATTGCCGGAC 
TATTCAACTA TTGAGATAAT GCGTGAAAAA CTCTTGATAG CTGCAAGAGA AGGGCAGCAG 
TCATTCCATC TTTCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TRIP12 ( XM_015276966.2 ) cDNA ORF clone, Gallus gallus(chicken) -> XP_015132452.1 Gallus gallus thyroid hormone receptor interactor 12 (TRIP12), transcript variant X3, mRNA.

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CloneID	OGa12502
Clone ID Related Accession (Same CDS sequence)	XM_015276964.1 , XM_015276968.2 , XM_015276967.1 , XM_015276968.1 , XM_004937064.3 , XM_015276964.2 , XM_015276966.2 , XM_004937064.2 , XM_015276966.1 , XM_015276967.2 , XM_015276965.1
Accession Version	XM_015276966.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6198bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-16
Organism	Gallus gallus(chicken)
Product	E3 ubiquitin-protein ligase TRIP12 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006096.5) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On May 17, 2018 this sequence version replaced XM_015276966.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Gallus gallus Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCCAACC GGCCTAATAA CAATCCAGGG GGGTCACTGC GACGTTCACA GAGGAACACT 
GCTGGGGCCC AGCCACAAGA CGACGCAGCA GGAGGAAGGT CACATTTAGG GCAGGCAAAA 
CATAAGGCAC ATAGCCCTCC TGAGAGTAGA AAATCTATTT CAAAGACACC CAAAGTGCAG 
TCTAATACTA CTTCTGAGCA GTCCAAGGGA CACTTTTCTA AAAGAGGCTG TAGTTCATCT 
GCTGTTTTAA TACCACAAGA AGATTCAGAA AGAGTCAGTA CTTCAGAAAA GCAAAAAACG 
GGGCAAGTGC CTAAGAAAGA CAATTCTCGA GGAGTTAAAC GCAGTGCTAG TCCAGATTAC 
AGCAGGACCA ATTCTCCTAG CTCTGCTAAA AAACCTAAAG CACTTCAACA CGCCGAAACT 
TCCTCGGAAA GTAACAAGCC ACATACTAAA TCCAAGAGAA GACACTTAGA CCAAGAACAG 
CAGAAGTCTA CACAATTGCC ATCAACAAGC AAGGCTCACA CCAGAAAGGG TGGAGCTGCT 
GGTAGCTCCC GAAGTCAGAA AAGGAAAAGG ACAGAGAGTA CGTCTTGTAT AAAGAGTCGT 
TCAGCAGTTG AATCAACTGG AGCTGAAGAG AAGTCAGCAA AATCCTCCAA GCTGGCTTCA 
AAATCGGTGA CCTCAGCCAA AGCTGGGTGT AGCACCATCA CTGATTCTTC TTCTTCAGCT 
TCTACATCCT CTTCCTCTTC TGCTGTTGCC TCTGCTTCTT CCACTGTTCC TCAGGGTGCC 
AGAGTGAAAC AGGGTAAGGA CCAGAATAAG GCTAGACGTT CACGTTCTGC GTCCAGCCCT 
AGTCCAAGAA GGAGCAGCAG GGACAAAGAA CAGAGCAAAA CAGGTGGCTC TTCAAAGTTT 
GACTGGGCTG CTCGATTCAG TCCAAAAGTT AGTCTCCCTA AAACAAAACT GTCTCTACCA 
GGCTCTTCTA AGTCAGAGAC CTCAAAACCT GGACCTTCAG GACTACAGGC TAAGCTAGCA 
AGCCTAAGAA AATCCACAAA GAAGCGCAGT GAATCACCAC CTGCTGAGCT CCCCAGTTTA 
CGGCGGAGCA CACGGCAAAA GACCACGGGC TCCTGTGCGA GTACCAGTCG GCGGGGCTCT 
GGCCTGGGCA AAAGAGGAGC AGCTGAAGCT CGCCGACAGG AGAAGATGGC TGATCCTGAC 
AACAACCAGG ATGGAGTTAA CTCTTCAGCT GCACGTACAG ATGAGACTCC CCAGGGAGCT 
GCAGCTTCTA GCTCTGTTGC TGGGGCTGTA GGTATGACAA CATCTGGAGA AAGTGAGTCA 
GATGACTCTG AGATGGGAAG ACTACAAGCT CTGTTAGAGG CTAGGGGTCT TCCTCCTCAC 
CTGTTTGGCC CTCTTGGTCC TCGGATGTCG CAGCTCTTCC ACAGGACAAT TGGAAGTGGA 
GCTAGTTCTA AAGCCCAGCA GCTTTTACAA GGTCTCCAAG CCACTGATGA AAGTCAGCAA 
CTACAGGCAG TTATTGAGAT GTGCCAGCTG TTGGTCATGG GAAATGAAGA AACACTGGGA 
GGATTTCCAG TCAAGAGTGT TGTGCCAGCT TTGATAACAT TGCTGCAGAT GGAGCATAAC 
TTTGACATCA TGAACCATGC TTGTCGAGCC TTAACATACA TGATGGAAGC ACTTCCCAGA 
TCATCTGCTG TAGTGGTAGA TGCAATTCCT GTCTTCTTGG AAAAGCTGCA AGTTATTCAG 
TGCATTGATG TGGCAGAGCA GGCACTGACA GCCCTAGAGA TGCTATCACG CAGGCATAGT 
AAAGCCATTC TGCAGGCAGG TGGGTTGGCA GACTGCTTGC TGTATCTGGA ATTCTTCAGT 
ATAAATGCGC AGAGGAATGC ACTAGCTATT GCTGCCAATT GCTGCCAGAG TATAACACCT 
GATGAGTTTC ACTTTGTGGC AGACTCTTTG CCACTGCTTA CACAAAGGTT AACCCATCAG 
GACAAGAAGT CTGTTGAAAG CACTTGTCTC TGTTTTGCAC GGCTGGTAGA CAATTTCCAG 
CATGAGGAGA ACTTGCTGCA GCAGGTTGCT TCCAAGGACT TGTTAACAAA CATCCAGCAA 
CTCTTGGTAG TGACACCTCC TATCCTGAGC TCAGGAATGT TCATCATGGT GGTGCGCATG 
TTTTCCTTAA TGTGTTCCAA TTGTCCAACG CTTGCAGTCC AACTTATGAA GCAAAACATT 
GCAGAAACAC TTCACTTTCT CCTTTGTGGA GCCTCAAATG GGAGTTGTCA AGAACAAATT 
GACCTTGTTC CACGAAGTCC TCAAGAACTC TATGAGCTTA CTTCTCTTAT CTGTGAACTG 
ATGCCTTGTC TGCCAAAAGA GGGGATATTT GCTGTTGATA CTATGCTAAA GAAAGGAAAT 
GCACAAAATA CAGATGGTGC GATATGGCAA TGGCGAGATG ACAGGGGTCT CTGGCATCCC 
TATAACAGGA TTGATAGTCG AATAATAGAG GCAGCTCATC AGGTTGGTGA GGATGAGATA 
AGCCTGTCGA CACTTGGACG TGTCTATACT ATTGATTTTA ATTCTATGCA GCAAATAAAT 
GAGGATACTG GAACAGCACG TGCCATTCAG CGGAAACCAA ACCCTTTAGC CAATACAAAC 
ACTAGTGGAC ATTCAGAATT GAAGAAGGAT GATGCTCGAG CACAGCTAAT GAAAGAAGAC 
CCAGAATTGG CAAAATCCTT TATCAAAACA CTGTTTGGTG TTCTTTATGA AGTGTATAGT 
TCTTCAGCTG GACCTGCTGT TAGACACAAG TGCCTTAGAG CAATACTTAG AATAATTTAT 
TTTGCTGATG CTGAACTCCT GAAGGATGTG CTGAAAAACC ATGCTGTGTC AAGTCACATT 
GCCTCCATGC TGTCAAGTCA AGACCTTAAG ATAGTAGTTG GAGCCCTGCA GATGGCAGAA 
ATCTTAATGC AGAAGCTACC TGACATTTTT AGTGTTTACT TCAGAAGAGA AGGAGTGATG 
CATCAAGTGA AAAACTTAGC GGAGTCTGAG GCTTTGCTAA CGAGCCCACC AAAAGTATGC 
ACTAATGGAT CAGGAACACT GGGTACCACT ACAACTATAA GTACTGGAAC AGCCACTGCT 
GCCACTAATG CAGCTGCAGA TTTGGGCTCT CCCAGCTTAC AACACAGCCG GGAGGATTCT 
TTAGATCTGA GCCCACAGGG ACGACTGAGT GATGTTCTGA AGAGAAAAAG ACTGCCAAAA 
CGAGGGCCAA GGAGACCAAA ATATTCTCCC CCAAGAGATG ATGACAAAGT AGACAATCAA 
GCAAAAAGCC CCACGACTAC TCAGTCTCCT AAATCTTCTT TCTTAGCAAG TTTAAATCCC 
AAAACATGGG GAAGATTAAG TACACAGTCT AACAGTAATA ACATTGAACC AGCAAGAACA 
GCAGGTGTAA GTGGTCTTGC AAGAGCTGCT TCTAAGGATA CAATTTCCAA TAACAGAGAA 
AAAATTAAGG GTTGGATTAA GGAGCAAGCC CATAAATTTG TAGACCGTTA TTTTAGTTCG 
GAAAACATGG ATGGAAGCAA TCCTGCACTA AATGTATTAC AGAGACTTTG CACTGCAACT 
GAACAACTTA ACCTCCAGGT GGATGGTGGA ACAGAGTGCC TTGTAGAAAT CCGTAGCATT 
GTCTCGGAGT CTGACGTCTC CTCATTTGAA ATCCAGCATA GTGGATTTGT GAAACAACTG 
CTGCTTTATT TGACATCTAA AAGTGAGAAA GATGCTGTAA GCAGGGATAT CAGATTGAAA 
AGATTTCTTC ATGTATTTTT CTCCTCTCCA CTTCCTGGAG AAGAACCCCT TGGAAGATTA 
GAGCCATTAG AAAATGCACC TTTGTTGGCA TTAGTCCATA AAATGAACAA TTGCCTCAGT 
CAGATGGAGC AGTTTCCAGT GAAAGTGCAT GACTTCCCCA GTGGAAATGG AACAGGGAGC 
AGTTTTTCTC TTAACAGAGG ATCCCAAGCT TTAAAATTCT TCAATACGCA TCAGTTAAAA 
TGCCAGCTGC AAAGACATCC AGACTGTGCT AATGTGAAAC AGTGGAAGGG TGGACCTGTG 
AAGATTGATC CTCTGGCTTT GGTTCAAGCC ATTGAAAGAT ATCTTGTTGT TAGAGGTTAT 
GGAAGAGTTA GAGAAGATGA TGAGGATAGT GATGATGATG GGTCAGATGA AGAAATAGAT 
GAATCCTTGG CTGCTCAATT CTTAAATTCA GGGAATGTGA GACATAGATT GCAGTTTTAC 
ATTGGGGATC ACTTGCTACC ATATAATATG ACTGTATATC AAGCAGTTAA GCTGTACAGT 
TTGCAAGCTG AGGAGGAGAG GGAATCGACT GATGATGAAA GCAATCCATT AGGAAGAGCT 
GGGATTTGGA CAAAAACACA TACCATTTGG TACAAACCTG TACGGGAGGA TGAAGATGGC 
AACAAAGACT GTGTTGGTGG TAAAAGAGGA AGAGCTCAAA CTGCTCCCAC AAAAACCTCA 
CCTAGGAATT CTAAAAAGCA TGATGAGTTG TGGCACGATG GTGTATGCCC TTCAGTATTG 
AATCCTTTAG AAGTTTACCT CATATCTACT CCTCCTGAAA ACATAACCTT TGAAGATCCT 
TCATTAGATG TTATTCTTCT TTTGAGAGTT TTACATGCTA TCAGTCGATA CTGGTATTAT 
TTGTATGATA ATGCAATATG CAAGGAGATT ATTCCAACTT CGGAATTTAT CAATAGTAAA 
CTGACAGCAA AAGCAAACAG ACAGCTTCAA GATCCACTGG TAATTATGAC AGGAAACATA 
CCGACTTGGC TGACAGAGCT TGGAAAAACA TGCCCATTTT TCTTTCCATT TGATACCCGG 
CAAATGCTGT TTTATGTAAC TGCTTTTGAT CGTGATCGAG CCATGCAAAG ACTACTGGAT 
ACTAATCCAG AAATCAATCA ATCAGATTCT CAGGATAGCA GAGTGGCACC ACGACTGGAC 
AGGAAAAAAC GCACTGTGAA CAGAGATGAG CTGTTGAAAC AGGCAGAATC TGTGATGCAG 
GATCTAGGCA GTTCAAGAGC CATGTTGGAA ATCCAGTATG AGAATGAAGT TGGCACAGGC 
CTGGGCCCTA CACTAGAGTT CTATGCACTT GTATCTCAGG AACTACAGAG AGCAGACTTG 
GGCCTTTGGA GGGGAGAAGA AGTAACTTTA GCCAATCCAA AAGGAAGCCA AGAAGGTACC 
AAGTATATTC ATAATCTCCA AGGCCTTTTT GCACTTCCTT TTGGTAGAAC AGCCAAGCCT 
GCTCACATTG CAAAAGTTAA AATGAAGTTC CGCTTTCTGG GAAAACTAAT GGCTAAGGCA 
ATCATGGATT TTAGACTGGT GGATCTTCCT CTTGGACTTC CTTTTTATAA ATGGATGTTA 
CGCCAGGAAA CTTCTTTGAC GTCACATGAC TTGTTCAGTA TTGATCCAGT AGTAGCCAAA 
TCAATATATC ACCTTGAAGA TATTGTAAGA CAAAAGAAAA GACTTGAGCA GGACAAAACA 
CAGACCAAAG AAAGTCTACA GTATGCATTG GAAACCCTGA CTATGAATGG CTGCTCAGTG 
GAGGATCTAG GGCTGGATTT CACACTTCCT GGATTTCCTA ACATCGAGCT GAAAAAAGGG 
GGAAAAGATA CACCAGTCAC CATCCACAAC TTGGAGGAGT ACCTCAGATT GGTTATATTC 
TGGGCACTTA ACGAAGGTGT TGCCAGACAG TTTGATTCTT TCAGAGATGG ATTTGAATCA 
GTCTTCCCCC TCAGTCATCT TCAATACTTT TATCCTGAGG AGTTGGAGCA ACTCTTATGT 
GGCAGCAAAA CAGACACATG GGATGCCAAG ACCTTAATGG AATGTTGCAG GCCAGATCAC 
GGTTATACAC ATGACAGTCG AGCAGTGAAG TATCTCTTTG AAATTCTCAG TAGTTTTGAT 
AGTGAGCAGC AGAGACTGTT TCTTCAGTTT GTGACCGGTA GTCCCAGGCT GCCAGTAGGA 
GGCTTTCGAA GTTTGAATCC TCCATTGACA ATTGTACGGA AGACATTTGA GTCTACAGAG 
AATCCAGATG ACTTCTTGCC CTCAGTAATG ACTTGTGTGA ACTATCTCAA ATTGCCGGAC 
TATTCAACTA TTGAGATAAT GCGTGAAAAA CTCTTGATAG CTGCAAGAGA AGGGCAGCAG 
TCATTCCATC TTTCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015132452.1
CDS	174..6371
Translation	MSNRPNNNPGGSLRRSQRNTAGAQPQDDAAGGRSHLGQAKHKAHSPPESRKSISKTPKVQSNTTSEQSKGHFSKRGCSSSAVLIPQEDSERVSTSEKQKTGQVPKKDNSRGVKRSASPDYSRTNSPSSAKKPKALQHAETSSESNKPHTKSKRRHLDQEQQKSTQLPSTSKAHTRKGGAAGSSRSQKRKRTESTSCIKSRSAVESTGAEEKSAKSSKLASKSVTSAKAGCSTITDSSSSASTSSSSSAVASASSTVPQGARVKQGKDQNKARRSRSASSPSPRRSSRDKEQSKTGGSSKFDWAARFSPKVSLPKTKLSLPGSSKSETSKPGPSGLQAKLASLRKSTKKRSESPPAELPSLRRSTRQKTTGSCASTSRRGSGLGKRGAAEARRQEKMADPDNNQDGVNSSAARTDETPQGAAASSSVAGAVGMTTSGESESDDSEMGRLQALLEARGLPPHLFGPLGPRMSQLFHRTIGSGASSKAQQLLQGLQATDESQQLQAVIEMCQLLVMGNEETLGGFPVKSVVPALITLLQMEHNFDIMNHACRALTYMMEALPRSSAVVVDAIPVFLEKLQVIQCIDVAEQALTALEMLSRRHSKAILQAGGLADCLLYLEFFSINAQRNALAIAANCCQSITPDEFHFVADSLPLLTQRLTHQDKKSVESTCLCFARLVDNFQHEENLLQQVASKDLLTNIQQLLVVTPPILSSGMFIMVVRMFSLMCSNCPTLAVQLMKQNIAETLHFLLCGASNGSCQEQIDLVPRSPQELYELTSLICELMPCLPKEGIFAVDTMLKKGNAQNTDGAIWQWRDDRGLWHPYNRIDSRIIEAAHQVGEDEISLSTLGRVYTIDFNSMQQINEDTGTARAIQRKPNPLANTNTSGHSELKKDDARAQLMKEDPELAKSFIKTLFGVLYEVYSSSAGPAVRHKCLRAILRIIYFADAELLKDVLKNHAVSSHIASMLSSQDLKIVVGALQMAEILMQKLPDIFSVYFRREGVMHQVKNLAESEALLTSPPKVCTNGSGTLGTTTTISTGTATAATNAAADLGSPSLQHSREDSLDLSPQGRLSDVLKRKRLPKRGPRRPKYSPPRDDDKVDNQAKSPTTTQSPKSSFLASLNPKTWGRLSTQSNSNNIEPARTAGVSGLARAASKDTISNNREKIKGWIKEQAHKFVDRYFSSENMDGSNPALNVLQRLCTATEQLNLQVDGGTECLVEIRSIVSESDVSSFEIQHSGFVKQLLLYLTSKSEKDAVSRDIRLKRFLHVFFSSPLPGEEPLGRLEPLENAPLLALVHKMNNCLSQMEQFPVKVHDFPSGNGTGSSFSLNRGSQALKFFNTHQLKCQLQRHPDCANVKQWKGGPVKIDPLALVQAIERYLVVRGYGRVREDDEDSDDDGSDEEIDESLAAQFLNSGNVRHRLQFYIGDHLLPYNMTVYQAVKLYSLQAEEERESTDDESNPLGRAGIWTKTHTIWYKPVREDEDGNKDCVGGKRGRAQTAPTKTSPRNSKKHDELWHDGVCPSVLNPLEVYLISTPPENITFEDPSLDVILLLRVLHAISRYWYYLYDNAICKEIIPTSEFINSKLTAKANRQLQDPLVIMTGNIPTWLTELGKTCPFFFPFDTRQMLFYVTAFDRDRAMQRLLDTNPEINQSDSQDSRVAPRLDRKKRTVNRDELLKQAESVMQDLGSSRAMLEIQYENEVGTGLGPTLEFYALVSQELQRADLGLWRGEEVTLANPKGSQEGTKYIHNLQGLFALPFGRTAKPAHIAKVKMKFRFLGKLMAKAIMDFRLVDLPLGLPFYKWMLRQETSLTSHDLFSIDPVVAKSIYHLEDIVRQKKRLEQDKTQTKESLQYALETLTMNGCSVEDLGLDFTLPGFPNIELKKGGKDTPVTIHNLEEYLRLVIFWALNEGVARQFDSFRDGFESVFPLSHLQYFYPEELEQLLCGSKTDTWDAKTLMECCRPDHGYTHDSRAVKYLFEILSSFDSEQQRLFLQFVTGSPRLPVGGFRSLNPPLTIVRKTFESTENPDDFLPSVMTCVNYLKLPDYSTIEIMREKLLIAAREGQQSFHLS

Target ORF information:

RefSeq Version	XM_015276966.2
Organism	Gallus gallus(chicken)
Definition	Gallus gallus thyroid hormone receptor interactor 12 (TRIP12), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015276966.2

ORF Insert Sequence:

ATGTCCAACC GGCCTAATAA CAATCCAGGG GGGTCACTGC GACGTTCACA GAGGAACACT 
GCTGGGGCCC AGCCACAAGA CGACGCAGCA GGAGGAAGGT CACATTTAGG GCAGGCAAAA 
CATAAGGCAC ATAGCCCTCC TGAGAGTAGA AAATCTATTT CAAAGACACC CAAAGTGCAG 
TCTAATACTA CTTCTGAGCA GTCCAAGGGA CACTTTTCTA AAAGAGGCTG TAGTTCATCT 
GCTGTTTTAA TACCACAAGA AGATTCAGAA AGAGTCAGTA CTTCAGAAAA GCAAAAAACG 
GGGCAAGTGC CTAAGAAAGA CAATTCTCGA GGAGTTAAAC GCAGTGCTAG TCCAGATTAC 
AGCAGGACCA ATTCTCCTAG CTCTGCTAAA AAACCTAAAG CACTTCAACA CGCCGAAACT 
TCCTCGGAAA GTAACAAGCC ACATACTAAA TCCAAGAGAA GACACTTAGA CCAAGAACAG 
CAGAAGTCTA CACAATTGCC ATCAACAAGC AAGGCTCACA CCAGAAAGGG TGGAGCTGCT 
GGTAGCTCCC GAAGTCAGAA AAGGAAAAGG ACAGAGAGTA CGTCTTGTAT AAAGAGTCGT 
TCAGCAGTTG AATCAACTGG AGCTGAAGAG AAGTCAGCAA AATCCTCCAA GCTGGCTTCA 
AAATCGGTGA CCTCAGCCAA AGCTGGGTGT AGCACCATCA CTGATTCTTC TTCTTCAGCT 
TCTACATCCT CTTCCTCTTC TGCTGTTGCC TCTGCTTCTT CCACTGTTCC TCAGGGTGCC 
AGAGTGAAAC AGGGTAAGGA CCAGAATAAG GCTAGACGTT CACGTTCTGC GTCCAGCCCT 
AGTCCAAGAA GGAGCAGCAG GGACAAAGAA CAGAGCAAAA CAGGTGGCTC TTCAAAGTTT 
GACTGGGCTG CTCGATTCAG TCCAAAAGTT AGTCTCCCTA AAACAAAACT GTCTCTACCA 
GGCTCTTCTA AGTCAGAGAC CTCAAAACCT GGACCTTCAG GACTACAGGC TAAGCTAGCA 
AGCCTAAGAA AATCCACAAA GAAGCGCAGT GAATCACCAC CTGCTGAGCT CCCCAGTTTA 
CGGCGGAGCA CACGGCAAAA GACCACGGGC TCCTGTGCGA GTACCAGTCG GCGGGGCTCT 
GGCCTGGGCA AAAGAGGAGC AGCTGAAGCT CGCCGACAGG AGAAGATGGC TGATCCTGAC 
AACAACCAGG ATGGAGTTAA CTCTTCAGCT GCACGTACAG ATGAGACTCC CCAGGGAGCT 
GCAGCTTCTA GCTCTGTTGC TGGGGCTGTA GGTATGACAA CATCTGGAGA AAGTGAGTCA 
GATGACTCTG AGATGGGAAG ACTACAAGCT CTGTTAGAGG CTAGGGGTCT TCCTCCTCAC 
CTGTTTGGCC CTCTTGGTCC TCGGATGTCG CAGCTCTTCC ACAGGACAAT TGGAAGTGGA 
GCTAGTTCTA AAGCCCAGCA GCTTTTACAA GGTCTCCAAG CCACTGATGA AAGTCAGCAA 
CTACAGGCAG TTATTGAGAT GTGCCAGCTG TTGGTCATGG GAAATGAAGA AACACTGGGA 
GGATTTCCAG TCAAGAGTGT TGTGCCAGCT TTGATAACAT TGCTGCAGAT GGAGCATAAC 
TTTGACATCA TGAACCATGC TTGTCGAGCC TTAACATACA TGATGGAAGC ACTTCCCAGA 
TCATCTGCTG TAGTGGTAGA TGCAATTCCT GTCTTCTTGG AAAAGCTGCA AGTTATTCAG 
TGCATTGATG TGGCAGAGCA GGCACTGACA GCCCTAGAGA TGCTATCACG CAGGCATAGT 
AAAGCCATTC TGCAGGCAGG TGGGTTGGCA GACTGCTTGC TGTATCTGGA ATTCTTCAGT 
ATAAATGCGC AGAGGAATGC ACTAGCTATT GCTGCCAATT GCTGCCAGAG TATAACACCT 
GATGAGTTTC ACTTTGTGGC AGACTCTTTG CCACTGCTTA CACAAAGGTT AACCCATCAG 
GACAAGAAGT CTGTTGAAAG CACTTGTCTC TGTTTTGCAC GGCTGGTAGA CAATTTCCAG 
CATGAGGAGA ACTTGCTGCA GCAGGTTGCT TCCAAGGACT TGTTAACAAA CATCCAGCAA 
CTCTTGGTAG TGACACCTCC TATCCTGAGC TCAGGAATGT TCATCATGGT GGTGCGCATG 
TTTTCCTTAA TGTGTTCCAA TTGTCCAACG CTTGCAGTCC AACTTATGAA GCAAAACATT 
GCAGAAACAC TTCACTTTCT CCTTTGTGGA GCCTCAAATG GGAGTTGTCA AGAACAAATT 
GACCTTGTTC CACGAAGTCC TCAAGAACTC TATGAGCTTA CTTCTCTTAT CTGTGAACTG 
ATGCCTTGTC TGCCAAAAGA GGGGATATTT GCTGTTGATA CTATGCTAAA GAAAGGAAAT 
GCACAAAATA CAGATGGTGC GATATGGCAA TGGCGAGATG ACAGGGGTCT CTGGCATCCC 
TATAACAGGA TTGATAGTCG AATAATAGAG GCAGCTCATC AGGTTGGTGA GGATGAGATA 
AGCCTGTCGA CACTTGGACG TGTCTATACT ATTGATTTTA ATTCTATGCA GCAAATAAAT 
GAGGATACTG GAACAGCACG TGCCATTCAG CGGAAACCAA ACCCTTTAGC CAATACAAAC 
ACTAGTGGAC ATTCAGAATT GAAGAAGGAT GATGCTCGAG CACAGCTAAT GAAAGAAGAC 
CCAGAATTGG CAAAATCCTT TATCAAAACA CTGTTTGGTG TTCTTTATGA AGTGTATAGT 
TCTTCAGCTG GACCTGCTGT TAGACACAAG TGCCTTAGAG CAATACTTAG AATAATTTAT 
TTTGCTGATG CTGAACTCCT GAAGGATGTG CTGAAAAACC ATGCTGTGTC AAGTCACATT 
GCCTCCATGC TGTCAAGTCA AGACCTTAAG ATAGTAGTTG GAGCCCTGCA GATGGCAGAA 
ATCTTAATGC AGAAGCTACC TGACATTTTT AGTGTTTACT TCAGAAGAGA AGGAGTGATG 
CATCAAGTGA AAAACTTAGC GGAGTCTGAG GCTTTGCTAA CGAGCCCACC AAAAGTATGC 
ACTAATGGAT CAGGAACACT GGGTACCACT ACAACTATAA GTACTGGAAC AGCCACTGCT 
GCCACTAATG CAGCTGCAGA TTTGGGCTCT CCCAGCTTAC AACACAGCCG GGAGGATTCT 
TTAGATCTGA GCCCACAGGG ACGACTGAGT GATGTTCTGA AGAGAAAAAG ACTGCCAAAA 
CGAGGGCCAA GGAGACCAAA ATATTCTCCC CCAAGAGATG ATGACAAAGT AGACAATCAA 
GCAAAAAGCC CCACGACTAC TCAGTCTCCT AAATCTTCTT TCTTAGCAAG TTTAAATCCC 
AAAACATGGG GAAGATTAAG TACACAGTCT AACAGTAATA ACATTGAACC AGCAAGAACA 
GCAGGTGTAA GTGGTCTTGC AAGAGCTGCT TCTAAGGATA CAATTTCCAA TAACAGAGAA 
AAAATTAAGG GTTGGATTAA GGAGCAAGCC CATAAATTTG TAGACCGTTA TTTTAGTTCG 
GAAAACATGG ATGGAAGCAA TCCTGCACTA AATGTATTAC AGAGACTTTG CACTGCAACT 
GAACAACTTA ACCTCCAGGT GGATGGTGGA ACAGAGTGCC TTGTAGAAAT CCGTAGCATT 
GTCTCGGAGT CTGACGTCTC CTCATTTGAA ATCCAGCATA GTGGATTTGT GAAACAACTG 
CTGCTTTATT TGACATCTAA AAGTGAGAAA GATGCTGTAA GCAGGGATAT CAGATTGAAA 
AGATTTCTTC ATGTATTTTT CTCCTCTCCA CTTCCTGGAG AAGAACCCCT TGGAAGATTA 
GAGCCATTAG AAAATGCACC TTTGTTGGCA TTAGTCCATA AAATGAACAA TTGCCTCAGT 
CAGATGGAGC AGTTTCCAGT GAAAGTGCAT GACTTCCCCA GTGGAAATGG AACAGGGAGC 
AGTTTTTCTC TTAACAGAGG ATCCCAAGCT TTAAAATTCT TCAATACGCA TCAGTTAAAA 
TGCCAGCTGC AAAGACATCC AGACTGTGCT AATGTGAAAC AGTGGAAGGG TGGACCTGTG 
AAGATTGATC CTCTGGCTTT GGTTCAAGCC ATTGAAAGAT ATCTTGTTGT TAGAGGTTAT 
GGAAGAGTTA GAGAAGATGA TGAGGATAGT GATGATGATG GGTCAGATGA AGAAATAGAT 
GAATCCTTGG CTGCTCAATT CTTAAATTCA GGGAATGTGA GACATAGATT GCAGTTTTAC 
ATTGGGGATC ACTTGCTACC ATATAATATG ACTGTATATC AAGCAGTTAA GCTGTACAGT 
TTGCAAGCTG AGGAGGAGAG GGAATCGACT GATGATGAAA GCAATCCATT AGGAAGAGCT 
GGGATTTGGA CAAAAACACA TACCATTTGG TACAAACCTG TACGGGAGGA TGAAGATGGC 
AACAAAGACT GTGTTGGTGG TAAAAGAGGA AGAGCTCAAA CTGCTCCCAC AAAAACCTCA 
CCTAGGAATT CTAAAAAGCA TGATGAGTTG TGGCACGATG GTGTATGCCC TTCAGTATTG 
AATCCTTTAG AAGTTTACCT CATATCTACT CCTCCTGAAA ACATAACCTT TGAAGATCCT 
TCATTAGATG TTATTCTTCT TTTGAGAGTT TTACATGCTA TCAGTCGATA CTGGTATTAT 
TTGTATGATA ATGCAATATG CAAGGAGATT ATTCCAACTT CGGAATTTAT CAATAGTAAA 
CTGACAGCAA AAGCAAACAG ACAGCTTCAA GATCCACTGG TAATTATGAC AGGAAACATA 
CCGACTTGGC TGACAGAGCT TGGAAAAACA TGCCCATTTT TCTTTCCATT TGATACCCGG 
CAAATGCTGT TTTATGTAAC TGCTTTTGAT CGTGATCGAG CCATGCAAAG ACTACTGGAT 
ACTAATCCAG AAATCAATCA ATCAGATTCT CAGGATAGCA GAGTGGCACC ACGACTGGAC 
AGGAAAAAAC GCACTGTGAA CAGAGATGAG CTGTTGAAAC AGGCAGAATC TGTGATGCAG 
GATCTAGGCA GTTCAAGAGC CATGTTGGAA ATCCAGTATG AGAATGAAGT TGGCACAGGC 
CTGGGCCCTA CACTAGAGTT CTATGCACTT GTATCTCAGG AACTACAGAG AGCAGACTTG 
GGCCTTTGGA GGGGAGAAGA AGTAACTTTA GCCAATCCAA AAGGAAGCCA AGAAGGTACC 
AAGTATATTC ATAATCTCCA AGGCCTTTTT GCACTTCCTT TTGGTAGAAC AGCCAAGCCT 
GCTCACATTG CAAAAGTTAA AATGAAGTTC CGCTTTCTGG GAAAACTAAT GGCTAAGGCA 
ATCATGGATT TTAGACTGGT GGATCTTCCT CTTGGACTTC CTTTTTATAA ATGGATGTTA 
CGCCAGGAAA CTTCTTTGAC GTCACATGAC TTGTTCAGTA TTGATCCAGT AGTAGCCAAA 
TCAATATATC ACCTTGAAGA TATTGTAAGA CAAAAGAAAA GACTTGAGCA GGACAAAACA 
CAGACCAAAG AAAGTCTACA GTATGCATTG GAAACCCTGA CTATGAATGG CTGCTCAGTG 
GAGGATCTAG GGCTGGATTT CACACTTCCT GGATTTCCTA ACATCGAGCT GAAAAAAGGG 
GGAAAAGATA CACCAGTCAC CATCCACAAC TTGGAGGAGT ACCTCAGATT GGTTATATTC 
TGGGCACTTA ACGAAGGTGT TGCCAGACAG TTTGATTCTT TCAGAGATGG ATTTGAATCA 
GTCTTCCCCC TCAGTCATCT TCAATACTTT TATCCTGAGG AGTTGGAGCA ACTCTTATGT 
GGCAGCAAAA CAGACACATG GGATGCCAAG ACCTTAATGG AATGTTGCAG GCCAGATCAC 
GGTTATACAC ATGACAGTCG AGCAGTGAAG TATCTCTTTG AAATTCTCAG TAGTTTTGAT 
AGTGAGCAGC AGAGACTGTT TCTTCAGTTT GTGACCGGTA GTCCCAGGCT GCCAGTAGGA 
GGCTTTCGAA GTTTGAATCC TCCATTGACA ATTGTACGGA AGACATTTGA GTCTACAGAG 
AATCCAGATG ACTTCTTGCC CTCAGTAATG ACTTGTGTGA ACTATCTCAA ATTGCCGGAC 
TATTCAACTA TTGAGATAAT GCGTGAAAAA CTCTTGATAG CTGCAAGAGA AGGGCAGCAG 
TCATTCCATC TTTCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TRIP12 ( XM_004937064.2 ) cDNA ORF clone, Gallus gallus(chicken) -> XP_004937121.1 Gallus gallus thyroid hormone receptor interactor 12 (TRIP12), transcript variant X1, mRNA.

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CloneID	OGa12502
Clone ID Related Accession (Same CDS sequence)	XM_015276964.1 , XM_015276968.2 , XM_015276967.1 , XM_015276968.1 , XM_004937064.3 , XM_015276964.2 , XM_015276966.2 , XM_004937064.2 , XM_015276966.1 , XM_015276967.2 , XM_015276965.1
Accession Version	XM_004937064.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6198bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-03
Organism	Gallus gallus(chicken)
Product	E3 ubiquitin-protein ligase TRIP12 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_001471743.3) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 4, 2016 this sequence version replaced XM_004937064.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Gallus gallus Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCCAACC GGCCTAATAA CAATCCAGGG GGGTCACTGC GACGTTCACA GAGGAACACT 
GCTGGGGCCC AGCCACAAGA CGACGCAGCA GGAGGAAGGT CACATTTAGG GCAGGCAAAA 
CATAAGGCAC ATAGCCCTCC TGAGAGTAGA AAATCTATTT CAAAGACACC CAAAGTGCAG 
TCTAATACTA CTTCTGAGCA GTCCAAGGGA CACTTTTCTA AAAGAGGCTG TAGTTCATCT 
GCTGTTTTAA TACCACAAGA AGATTCAGAA AGAGTCAGTA CTTCAGAAAA GCAAAAAACG 
GGGCAAGTGC CTAAGAAAGA CAATTCTCGA GGAGTTAAAC GCAGTGCTAG TCCAGATTAC 
AGCAGGACCA ATTCTCCTAG CTCTGCTAAA AAACCTAAAG CACTTCAACA CGCCGAAACT 
TCCTCGGAAA GTAACAAGCC ACATACTAAA TCCAAGAGAA GACACTTAGA CCAAGAACAG 
CAGAAGTCTA CACAATTGCC ATCAACAAGC AAGGCTCACA CCAGAAAGGG TGGAGCTGCT 
GGTAGCTCCC GAAGTCAGAA AAGGAAAAGG ACAGAGAGTA CGTCTTGTAT AAAGAGTCGT 
TCAGCAGTTG AATCAACTGG AGCTGAAGAG AAGTCAGCAA AATCCTCCAA GCTGGCTTCA 
AAATCGGTGA CCTCAGCCAA AGCTGGGTGT AGCACCATCA CTGATTCTTC TTCTTCAGCT 
TCTACATCCT CTTCCTCTTC TGCTGTTGCC TCTGCTTCTT CCACTGTTCC TCAGGGTGCC 
AGAGTGAAAC AGGGTAAGGA CCAGAATAAG GCTAGACGTT CACGTTCTGC GTCCAGCCCT 
AGTCCAAGAA GGAGCAGCAG GGACAAAGAA CAGAGCAAAA CAGGTGGCTC TTCAAAGTTT 
GACTGGGCTG CTCGATTCAG TCCAAAAGTT AGTCTCCCTA AAACAAAACT GTCTCTACCA 
GGCTCTTCTA AGTCAGAGAC CTCAAAACCT GGACCTTCAG GACTACAGGC TAAGCTAGCA 
AGCCTAAGAA AATCCACAAA GAAGCGCAGT GAATCACCAC CTGCTGAGCT CCCCAGTTTA 
CGGCGGAGCA CACGGCAAAA GACCACGGGC TCCTGTGCGA GTACCAGTCG GCGGGGCTCT 
GGCCTGGGCA AAAGAGGAGC AGCTGAAGCT CGCCGACAGG AGAAGATGGC TGATCCTGAC 
AACAACCAGG ATGGAGTTAA CTCTTCAGCT GCACGTACAG ATGAGACTCC CCAGGGAGCT 
GCAGCTTCTA GCTCTGTTGC TGGGGCTGTA GGTATGACAA CATCTGGAGA AAGTGAGTCA 
GATGACTCTG AGATGGGAAG ACTACAAGCT CTGTTAGAGG CTAGGGGTCT TCCTCCTCAC 
CTGTTTGGCC CTCTTGGTCC TCGGATGTCG CAGCTCTTCC ACAGGACAAT TGGAAGTGGA 
GCTAGTTCTA AAGCCCAGCA GCTTTTACAA GGTCTCCAAG CCACTGATGA AAGTCAGCAA 
CTACAGGCAG TTATTGAGAT GTGCCAGCTG TTGGTCATGG GAAATGAAGA AACACTGGGA 
GGATTTCCAG TCAAGAGTGT TGTGCCAGCT TTGATAACAT TGCTGCAGAT GGAGCATAAC 
TTTGACATCA TGAACCATGC TTGTCGAGCC TTAACATACA TGATGGAAGC ACTTCCCAGA 
TCATCTGCTG TAGTGGTAGA TGCAATTCCT GTCTTCTTGG AAAAGCTGCA AGTTATTCAG 
TGCATTGATG TGGCAGAGCA GGCACTGACA GCCCTAGAGA TGCTATCACG CAGGCATAGT 
AAAGCCATTC TGCAGGCAGG TGGGTTGGCA GACTGCTTGC TGTATCTGGA ATTCTTCAGT 
ATAAATGCGC AGAGGAATGC ACTAGCTATT GCTGCCAATT GCTGCCAGAG TATAACACCT 
GATGAGTTTC ACTTTGTGGC AGACTCTTTG CCACTGCTTA CACAAAGGTT AACCCATCAG 
GACAAGAAGT CTGTTGAAAG CACTTGTCTC TGTTTTGCAC GGCTGGTAGA CAATTTCCAG 
CATGAGGAGA ACTTGCTGCA GCAGGTTGCT TCCAAGGACT TGTTAACAAA CATCCAGCAA 
CTCTTGGTAG TGACACCTCC TATCCTGAGC TCAGGAATGT TCATCATGGT GGTGCGCATG 
TTTTCCTTAA TGTGTTCCAA TTGTCCAACG CTTGCAGTCC AACTTATGAA GCAAAACATT 
GCAGAAACAC TTCACTTTCT CCTTTGTGGA GCCTCAAATG GGAGTTGTCA AGAACAAATT 
GACCTTGTTC CACGAAGTCC TCAAGAACTC TATGAGCTTA CTTCTCTTAT CTGTGAACTG 
ATGCCTTGTC TGCCAAAAGA GGGGATATTT GCTGTTGATA CTATGCTAAA GAAAGGAAAT 
GCACAAAATA CAGATGGTGC GATATGGCAA TGGCGAGATG ACAGGGGTCT CTGGCATCCC 
TATAACAGGA TTGATAGTCG AATAATAGAG GCAGCTCATC AGGTTGGTGA GGATGAGATA 
AGCCTGTCGA CACTTGGACG TGTCTATACT ATTGATTTTA ATTCTATGCA GCAAATAAAT 
GAGGATACTG GAACAGCACG TGCCATTCAG CGGAAACCAA ACCCTTTAGC CAATACAAAC 
ACTAGTGGAC ATTCAGAATT GAAGAAGGAT GATGCTCGAG CACAGCTAAT GAAAGAAGAC 
CCAGAATTGG CAAAATCCTT TATCAAAACA CTGTTTGGTG TTCTTTATGA AGTGTATAGT 
TCTTCAGCTG GACCTGCTGT TAGACACAAG TGCCTTAGAG CAATACTTAG AATAATTTAT 
TTTGCTGATG CTGAACTCCT GAAGGATGTG CTGAAAAACC ATGCTGTGTC AAGTCACATT 
GCCTCCATGC TGTCAAGTCA AGACCTTAAG ATAGTAGTTG GAGCCCTGCA GATGGCAGAA 
ATCTTAATGC AGAAGCTACC TGACATTTTT AGTGTTTACT TCAGAAGAGA AGGAGTGATG 
CATCAAGTGA AAAACTTAGC GGAGTCTGAG GCTTTGCTAA CGAGCCCACC AAAAGTATGC 
ACTAATGGAT CAGGAACACT GGGTACCACT ACAACTATAA GTACTGGAAC AGCCACTGCT 
GCCACTAATG CAGCTGCAGA TTTGGGCTCT CCCAGCTTAC AACACAGCCG GGAGGATTCT 
TTAGATCTGA GCCCACAGGG ACGACTGAGT GATGTTCTGA AGAGAAAAAG ACTGCCAAAA 
CGAGGGCCAA GGAGACCAAA ATATTCTCCC CCAAGAGATG ATGACAAAGT AGACAATCAA 
GCAAAAAGCC CCACGACTAC TCAGTCTCCT AAATCTTCTT TCTTAGCAAG TTTAAATCCC 
AAAACATGGG GAAGATTAAG TACACAGTCT AACAGTAATA ACATTGAACC AGCAAGAACA 
GCAGGTGTAA GTGGTCTTGC AAGAGCTGCT TCTAAGGATA CAATTTCCAA TAACAGAGAA 
AAAATTAAGG GTTGGATTAA GGAGCAAGCC CATAAATTTG TAGACCGTTA TTTTAGTTCG 
GAAAACATGG ATGGAAGCAA TCCTGCACTA AATGTATTAC AGAGACTTTG CACTGCAACT 
GAACAACTTA ACCTCCAGGT GGATGGTGGA ACAGAGTGCC TTGTAGAAAT CCGTAGCATT 
GTCTCGGAGT CTGACGTCTC CTCATTTGAA ATCCAGCATA GTGGATTTGT GAAACAACTG 
CTGCTTTATT TGACATCTAA AAGTGAGAAA GATGCTGTAA GCAGGGATAT CAGATTGAAA 
AGATTTCTTC ATGTATTTTT CTCCTCTCCA CTTCCTGGAG AAGAACCCCT TGGAAGATTA 
GAGCCATTAG AAAATGCACC TTTGTTGGCA TTAGTCCATA AAATGAACAA TTGCCTCAGT 
CAGATGGAGC AGTTTCCAGT GAAAGTGCAT GACTTCCCCA GTGGAAATGG AACAGGGAGC 
AGTTTTTCTC TTAACAGAGG ATCCCAAGCT TTAAAATTCT TCAATACGCA TCAGTTAAAA 
TGCCAGCTGC AAAGACATCC AGACTGTGCT AATGTGAAAC AGTGGAAGGG TGGACCTGTG 
AAGATTGATC CTCTGGCTTT GGTTCAAGCC ATTGAAAGAT ATCTTGTTGT TAGAGGTTAT 
GGAAGAGTTA GAGAAGATGA TGAGGATAGT GATGATGATG GGTCAGATGA AGAAATAGAT 
GAATCCTTGG CTGCTCAATT CTTAAATTCA GGGAATGTGA GACATAGATT GCAGTTTTAC 
ATTGGGGATC ACTTGCTACC ATATAATATG ACTGTATATC AAGCAGTTAA GCTGTACAGT 
TTGCAAGCTG AGGAGGAGAG GGAATCGACT GATGATGAAA GCAATCCATT AGGAAGAGCT 
GGGATTTGGA CAAAAACACA TACCATTTGG TACAAACCTG TACGGGAGGA TGAAGATGGC 
AACAAAGACT GTGTTGGTGG TAAAAGAGGA AGAGCTCAAA CTGCTCCCAC AAAAACCTCA 
CCTAGGAATT CTAAAAAGCA TGATGAGTTG TGGCACGATG GTGTATGCCC TTCAGTATTG 
AATCCTTTAG AAGTTTACCT CATATCTACT CCTCCTGAAA ACATAACCTT TGAAGATCCT 
TCATTAGATG TTATTCTTCT TTTGAGAGTT TTACATGCTA TCAGTCGATA CTGGTATTAT 
TTGTATGATA ATGCAATATG CAAGGAGATT ATTCCAACTT CGGAATTTAT CAATAGTAAA 
CTGACAGCAA AAGCAAACAG ACAGCTTCAA GATCCACTGG TAATTATGAC AGGAAACATA 
CCGACTTGGC TGACAGAGCT TGGAAAAACA TGCCCATTTT TCTTTCCATT TGATACCCGG 
CAAATGCTGT TTTATGTAAC TGCTTTTGAT CGTGATCGAG CCATGCAAAG ACTACTGGAT 
ACTAATCCAG AAATCAATCA ATCAGATTCT CAGGATAGCA GAGTGGCACC ACGACTGGAC 
AGGAAAAAAC GCACTGTGAA CAGAGATGAG CTGTTGAAAC AGGCAGAATC TGTGATGCAG 
GATCTAGGCA GTTCAAGAGC CATGTTGGAA ATCCAGTATG AGAATGAAGT TGGCACAGGC 
CTGGGCCCTA CACTAGAGTT CTATGCACTT GTATCTCAGG AACTACAGAG AGCAGACTTG 
GGCCTTTGGA GGGGAGAAGA AGTAACTTTA GCCAATCCAA AAGGAAGCCA AGAAGGTACC 
AAGTATATTC ATAATCTCCA AGGCCTTTTT GCACTTCCTT TTGGTAGAAC AGCCAAGCCT 
GCTCACATTG CAAAAGTTAA AATGAAGTTC CGCTTTCTGG GAAAACTAAT GGCTAAGGCA 
ATCATGGATT TTAGACTGGT GGATCTTCCT CTTGGACTTC CTTTTTATAA ATGGATGTTA 
CGCCAGGAAA CTTCTTTGAC GTCACATGAC TTGTTCAGTA TTGATCCAGT AGTAGCCAAA 
TCAATATATC ACCTTGAAGA TATTGTAAGA CAAAAGAAAA GACTTGAGCA GGACAAAACA 
CAGACCAAAG AAAGTCTACA GTATGCATTG GAAACCCTGA CTATGAATGG CTGCTCAGTG 
GAGGATCTAG GGCTGGATTT CACACTTCCT GGATTTCCTA ACATCGAGCT GAAAAAAGGG 
GGAAAAGATA CACCAGTCAC CATCCACAAC TTGGAGGAGT ACCTCAGATT GGTTATATTC 
TGGGCACTTA ACGAAGGTGT TGCCAGACAG TTTGATTCTT TCAGAGATGG ATTTGAATCA 
GTCTTCCCCC TCAGTCATCT TCAATACTTT TATCCTGAGG AGTTGGAGCA ACTCTTATGT 
GGCAGCAAAA CAGACACATG GGATGCCAAG ACCTTAATGG AATGTTGCAG GCCAGATCAC 
GGTTATACAC ATGACAGTCG AGCAGTGAAG TATCTCTTTG AAATTCTCAG TAGTTTTGAT 
AGTGAGCAGC AGAGACTGTT TCTTCAGTTT GTGACCGGTA GTCCCAGGCT GCCAGTAGGA 
GGCTTTCGAA GTTTGAATCC TCCATTGACA ATTGTACGGA AGACATTTGA GTCTACAGAG 
AATCCAGATG ACTTCTTGCC CTCAGTAATG ACTTGTGTGA ACTATCTCAA ATTGCCGGAC 
TATTCAACTA TTGAGATAAT GCGTGAAAAA CTCTTGATAG CTGCAAGAGA AGGGCAGCAG 
TCATTCCATC TTTCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_004937121.1
CDS	150..6347
Translation	MSNRPNNNPGGSLRRSQRNTAGAQPQDDAAGGRSHLGQAKHKAHSPPESRKSISKTPKVQSNTTSEQSKGHFSKRGCSSSAVLIPQEDSERVSTSEKQKTGQVPKKDNSRGVKRSASPDYSRTNSPSSAKKPKALQHAETSSESNKPHTKSKRRHLDQEQQKSTQLPSTSKAHTRKGGAAGSSRSQKRKRTESTSCIKSRSAVESTGAEEKSAKSSKLASKSVTSAKAGCSTITDSSSSASTSSSSSAVASASSTVPQGARVKQGKDQNKARRSRSASSPSPRRSSRDKEQSKTGGSSKFDWAARFSPKVSLPKTKLSLPGSSKSETSKPGPSGLQAKLASLRKSTKKRSESPPAELPSLRRSTRQKTTGSCASTSRRGSGLGKRGAAEARRQEKMADPDNNQDGVNSSAARTDETPQGAAASSSVAGAVGMTTSGESESDDSEMGRLQALLEARGLPPHLFGPLGPRMSQLFHRTIGSGASSKAQQLLQGLQATDESQQLQAVIEMCQLLVMGNEETLGGFPVKSVVPALITLLQMEHNFDIMNHACRALTYMMEALPRSSAVVVDAIPVFLEKLQVIQCIDVAEQALTALEMLSRRHSKAILQAGGLADCLLYLEFFSINAQRNALAIAANCCQSITPDEFHFVADSLPLLTQRLTHQDKKSVESTCLCFARLVDNFQHEENLLQQVASKDLLTNIQQLLVVTPPILSSGMFIMVVRMFSLMCSNCPTLAVQLMKQNIAETLHFLLCGASNGSCQEQIDLVPRSPQELYELTSLICELMPCLPKEGIFAVDTMLKKGNAQNTDGAIWQWRDDRGLWHPYNRIDSRIIEAAHQVGEDEISLSTLGRVYTIDFNSMQQINEDTGTARAIQRKPNPLANTNTSGHSELKKDDARAQLMKEDPELAKSFIKTLFGVLYEVYSSSAGPAVRHKCLRAILRIIYFADAELLKDVLKNHAVSSHIASMLSSQDLKIVVGALQMAEILMQKLPDIFSVYFRREGVMHQVKNLAESEALLTSPPKVCTNGSGTLGTTTTISTGTATAATNAAADLGSPSLQHSREDSLDLSPQGRLSDVLKRKRLPKRGPRRPKYSPPRDDDKVDNQAKSPTTTQSPKSSFLASLNPKTWGRLSTQSNSNNIEPARTAGVSGLARAASKDTISNNREKIKGWIKEQAHKFVDRYFSSENMDGSNPALNVLQRLCTATEQLNLQVDGGTECLVEIRSIVSESDVSSFEIQHSGFVKQLLLYLTSKSEKDAVSRDIRLKRFLHVFFSSPLPGEEPLGRLEPLENAPLLALVHKMNNCLSQMEQFPVKVHDFPSGNGTGSSFSLNRGSQALKFFNTHQLKCQLQRHPDCANVKQWKGGPVKIDPLALVQAIERYLVVRGYGRVREDDEDSDDDGSDEEIDESLAAQFLNSGNVRHRLQFYIGDHLLPYNMTVYQAVKLYSLQAEEERESTDDESNPLGRAGIWTKTHTIWYKPVREDEDGNKDCVGGKRGRAQTAPTKTSPRNSKKHDELWHDGVCPSVLNPLEVYLISTPPENITFEDPSLDVILLLRVLHAISRYWYYLYDNAICKEIIPTSEFINSKLTAKANRQLQDPLVIMTGNIPTWLTELGKTCPFFFPFDTRQMLFYVTAFDRDRAMQRLLDTNPEINQSDSQDSRVAPRLDRKKRTVNRDELLKQAESVMQDLGSSRAMLEIQYENEVGTGLGPTLEFYALVSQELQRADLGLWRGEEVTLANPKGSQEGTKYIHNLQGLFALPFGRTAKPAHIAKVKMKFRFLGKLMAKAIMDFRLVDLPLGLPFYKWMLRQETSLTSHDLFSIDPVVAKSIYHLEDIVRQKKRLEQDKTQTKESLQYALETLTMNGCSVEDLGLDFTLPGFPNIELKKGGKDTPVTIHNLEEYLRLVIFWALNEGVARQFDSFRDGFESVFPLSHLQYFYPEELEQLLCGSKTDTWDAKTLMECCRPDHGYTHDSRAVKYLFEILSSFDSEQQRLFLQFVTGSPRLPVGGFRSLNPPLTIVRKTFESTENPDDFLPSVMTCVNYLKLPDYSTIEIMREKLLIAAREGQQSFHLS

Target ORF information:

RefSeq Version	XM_004937064.2
Organism	Gallus gallus(chicken)
Definition	Gallus gallus thyroid hormone receptor interactor 12 (TRIP12), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_004937064.2

ORF Insert Sequence:

ATGTCCAACC GGCCTAATAA CAATCCAGGG GGGTCACTGC GACGTTCACA GAGGAACACT 
GCTGGGGCCC AGCCACAAGA CGACGCAGCA GGAGGAAGGT CACATTTAGG GCAGGCAAAA 
CATAAGGCAC ATAGCCCTCC TGAGAGTAGA AAATCTATTT CAAAGACACC CAAAGTGCAG 
TCTAATACTA CTTCTGAGCA GTCCAAGGGA CACTTTTCTA AAAGAGGCTG TAGTTCATCT 
GCTGTTTTAA TACCACAAGA AGATTCAGAA AGAGTCAGTA CTTCAGAAAA GCAAAAAACG 
GGGCAAGTGC CTAAGAAAGA CAATTCTCGA GGAGTTAAAC GCAGTGCTAG TCCAGATTAC 
AGCAGGACCA ATTCTCCTAG CTCTGCTAAA AAACCTAAAG CACTTCAACA CGCCGAAACT 
TCCTCGGAAA GTAACAAGCC ACATACTAAA TCCAAGAGAA GACACTTAGA CCAAGAACAG 
CAGAAGTCTA CACAATTGCC ATCAACAAGC AAGGCTCACA CCAGAAAGGG TGGAGCTGCT 
GGTAGCTCCC GAAGTCAGAA AAGGAAAAGG ACAGAGAGTA CGTCTTGTAT AAAGAGTCGT 
TCAGCAGTTG AATCAACTGG AGCTGAAGAG AAGTCAGCAA AATCCTCCAA GCTGGCTTCA 
AAATCGGTGA CCTCAGCCAA AGCTGGGTGT AGCACCATCA CTGATTCTTC TTCTTCAGCT 
TCTACATCCT CTTCCTCTTC TGCTGTTGCC TCTGCTTCTT CCACTGTTCC TCAGGGTGCC 
AGAGTGAAAC AGGGTAAGGA CCAGAATAAG GCTAGACGTT CACGTTCTGC GTCCAGCCCT 
AGTCCAAGAA GGAGCAGCAG GGACAAAGAA CAGAGCAAAA CAGGTGGCTC TTCAAAGTTT 
GACTGGGCTG CTCGATTCAG TCCAAAAGTT AGTCTCCCTA AAACAAAACT GTCTCTACCA 
GGCTCTTCTA AGTCAGAGAC CTCAAAACCT GGACCTTCAG GACTACAGGC TAAGCTAGCA 
AGCCTAAGAA AATCCACAAA GAAGCGCAGT GAATCACCAC CTGCTGAGCT CCCCAGTTTA 
CGGCGGAGCA CACGGCAAAA GACCACGGGC TCCTGTGCGA GTACCAGTCG GCGGGGCTCT 
GGCCTGGGCA AAAGAGGAGC AGCTGAAGCT CGCCGACAGG AGAAGATGGC TGATCCTGAC 
AACAACCAGG ATGGAGTTAA CTCTTCAGCT GCACGTACAG ATGAGACTCC CCAGGGAGCT 
GCAGCTTCTA GCTCTGTTGC TGGGGCTGTA GGTATGACAA CATCTGGAGA AAGTGAGTCA 
GATGACTCTG AGATGGGAAG ACTACAAGCT CTGTTAGAGG CTAGGGGTCT TCCTCCTCAC 
CTGTTTGGCC CTCTTGGTCC TCGGATGTCG CAGCTCTTCC ACAGGACAAT TGGAAGTGGA 
GCTAGTTCTA AAGCCCAGCA GCTTTTACAA GGTCTCCAAG CCACTGATGA AAGTCAGCAA 
CTACAGGCAG TTATTGAGAT GTGCCAGCTG TTGGTCATGG GAAATGAAGA AACACTGGGA 
GGATTTCCAG TCAAGAGTGT TGTGCCAGCT TTGATAACAT TGCTGCAGAT GGAGCATAAC 
TTTGACATCA TGAACCATGC TTGTCGAGCC TTAACATACA TGATGGAAGC ACTTCCCAGA 
TCATCTGCTG TAGTGGTAGA TGCAATTCCT GTCTTCTTGG AAAAGCTGCA AGTTATTCAG 
TGCATTGATG TGGCAGAGCA GGCACTGACA GCCCTAGAGA TGCTATCACG CAGGCATAGT 
AAAGCCATTC TGCAGGCAGG TGGGTTGGCA GACTGCTTGC TGTATCTGGA ATTCTTCAGT 
ATAAATGCGC AGAGGAATGC ACTAGCTATT GCTGCCAATT GCTGCCAGAG TATAACACCT 
GATGAGTTTC ACTTTGTGGC AGACTCTTTG CCACTGCTTA CACAAAGGTT AACCCATCAG 
GACAAGAAGT CTGTTGAAAG CACTTGTCTC TGTTTTGCAC GGCTGGTAGA CAATTTCCAG 
CATGAGGAGA ACTTGCTGCA GCAGGTTGCT TCCAAGGACT TGTTAACAAA CATCCAGCAA 
CTCTTGGTAG TGACACCTCC TATCCTGAGC TCAGGAATGT TCATCATGGT GGTGCGCATG 
TTTTCCTTAA TGTGTTCCAA TTGTCCAACG CTTGCAGTCC AACTTATGAA GCAAAACATT 
GCAGAAACAC TTCACTTTCT CCTTTGTGGA GCCTCAAATG GGAGTTGTCA AGAACAAATT 
GACCTTGTTC CACGAAGTCC TCAAGAACTC TATGAGCTTA CTTCTCTTAT CTGTGAACTG 
ATGCCTTGTC TGCCAAAAGA GGGGATATTT GCTGTTGATA CTATGCTAAA GAAAGGAAAT 
GCACAAAATA CAGATGGTGC GATATGGCAA TGGCGAGATG ACAGGGGTCT CTGGCATCCC 
TATAACAGGA TTGATAGTCG AATAATAGAG GCAGCTCATC AGGTTGGTGA GGATGAGATA 
AGCCTGTCGA CACTTGGACG TGTCTATACT ATTGATTTTA ATTCTATGCA GCAAATAAAT 
GAGGATACTG GAACAGCACG TGCCATTCAG CGGAAACCAA ACCCTTTAGC CAATACAAAC 
ACTAGTGGAC ATTCAGAATT GAAGAAGGAT GATGCTCGAG CACAGCTAAT GAAAGAAGAC 
CCAGAATTGG CAAAATCCTT TATCAAAACA CTGTTTGGTG TTCTTTATGA AGTGTATAGT 
TCTTCAGCTG GACCTGCTGT TAGACACAAG TGCCTTAGAG CAATACTTAG AATAATTTAT 
TTTGCTGATG CTGAACTCCT GAAGGATGTG CTGAAAAACC ATGCTGTGTC AAGTCACATT 
GCCTCCATGC TGTCAAGTCA AGACCTTAAG ATAGTAGTTG GAGCCCTGCA GATGGCAGAA 
ATCTTAATGC AGAAGCTACC TGACATTTTT AGTGTTTACT TCAGAAGAGA AGGAGTGATG 
CATCAAGTGA AAAACTTAGC GGAGTCTGAG GCTTTGCTAA CGAGCCCACC AAAAGTATGC 
ACTAATGGAT CAGGAACACT GGGTACCACT ACAACTATAA GTACTGGAAC AGCCACTGCT 
GCCACTAATG CAGCTGCAGA TTTGGGCTCT CCCAGCTTAC AACACAGCCG GGAGGATTCT 
TTAGATCTGA GCCCACAGGG ACGACTGAGT GATGTTCTGA AGAGAAAAAG ACTGCCAAAA 
CGAGGGCCAA GGAGACCAAA ATATTCTCCC CCAAGAGATG ATGACAAAGT AGACAATCAA 
GCAAAAAGCC CCACGACTAC TCAGTCTCCT AAATCTTCTT TCTTAGCAAG TTTAAATCCC 
AAAACATGGG GAAGATTAAG TACACAGTCT AACAGTAATA ACATTGAACC AGCAAGAACA 
GCAGGTGTAA GTGGTCTTGC AAGAGCTGCT TCTAAGGATA CAATTTCCAA TAACAGAGAA 
AAAATTAAGG GTTGGATTAA GGAGCAAGCC CATAAATTTG TAGACCGTTA TTTTAGTTCG 
GAAAACATGG ATGGAAGCAA TCCTGCACTA AATGTATTAC AGAGACTTTG CACTGCAACT 
GAACAACTTA ACCTCCAGGT GGATGGTGGA ACAGAGTGCC TTGTAGAAAT CCGTAGCATT 
GTCTCGGAGT CTGACGTCTC CTCATTTGAA ATCCAGCATA GTGGATTTGT GAAACAACTG 
CTGCTTTATT TGACATCTAA AAGTGAGAAA GATGCTGTAA GCAGGGATAT CAGATTGAAA 
AGATTTCTTC ATGTATTTTT CTCCTCTCCA CTTCCTGGAG AAGAACCCCT TGGAAGATTA 
GAGCCATTAG AAAATGCACC TTTGTTGGCA TTAGTCCATA AAATGAACAA TTGCCTCAGT 
CAGATGGAGC AGTTTCCAGT GAAAGTGCAT GACTTCCCCA GTGGAAATGG AACAGGGAGC 
AGTTTTTCTC TTAACAGAGG ATCCCAAGCT TTAAAATTCT TCAATACGCA TCAGTTAAAA 
TGCCAGCTGC AAAGACATCC AGACTGTGCT AATGTGAAAC AGTGGAAGGG TGGACCTGTG 
AAGATTGATC CTCTGGCTTT GGTTCAAGCC ATTGAAAGAT ATCTTGTTGT TAGAGGTTAT 
GGAAGAGTTA GAGAAGATGA TGAGGATAGT GATGATGATG GGTCAGATGA AGAAATAGAT 
GAATCCTTGG CTGCTCAATT CTTAAATTCA GGGAATGTGA GACATAGATT GCAGTTTTAC 
ATTGGGGATC ACTTGCTACC ATATAATATG ACTGTATATC AAGCAGTTAA GCTGTACAGT 
TTGCAAGCTG AGGAGGAGAG GGAATCGACT GATGATGAAA GCAATCCATT AGGAAGAGCT 
GGGATTTGGA CAAAAACACA TACCATTTGG TACAAACCTG TACGGGAGGA TGAAGATGGC 
AACAAAGACT GTGTTGGTGG TAAAAGAGGA AGAGCTCAAA CTGCTCCCAC AAAAACCTCA 
CCTAGGAATT CTAAAAAGCA TGATGAGTTG TGGCACGATG GTGTATGCCC TTCAGTATTG 
AATCCTTTAG AAGTTTACCT CATATCTACT CCTCCTGAAA ACATAACCTT TGAAGATCCT 
TCATTAGATG TTATTCTTCT TTTGAGAGTT TTACATGCTA TCAGTCGATA CTGGTATTAT 
TTGTATGATA ATGCAATATG CAAGGAGATT ATTCCAACTT CGGAATTTAT CAATAGTAAA 
CTGACAGCAA AAGCAAACAG ACAGCTTCAA GATCCACTGG TAATTATGAC AGGAAACATA 
CCGACTTGGC TGACAGAGCT TGGAAAAACA TGCCCATTTT TCTTTCCATT TGATACCCGG 
CAAATGCTGT TTTATGTAAC TGCTTTTGAT CGTGATCGAG CCATGCAAAG ACTACTGGAT 
ACTAATCCAG AAATCAATCA ATCAGATTCT CAGGATAGCA GAGTGGCACC ACGACTGGAC 
AGGAAAAAAC GCACTGTGAA CAGAGATGAG CTGTTGAAAC AGGCAGAATC TGTGATGCAG 
GATCTAGGCA GTTCAAGAGC CATGTTGGAA ATCCAGTATG AGAATGAAGT TGGCACAGGC 
CTGGGCCCTA CACTAGAGTT CTATGCACTT GTATCTCAGG AACTACAGAG AGCAGACTTG 
GGCCTTTGGA GGGGAGAAGA AGTAACTTTA GCCAATCCAA AAGGAAGCCA AGAAGGTACC 
AAGTATATTC ATAATCTCCA AGGCCTTTTT GCACTTCCTT TTGGTAGAAC AGCCAAGCCT 
GCTCACATTG CAAAAGTTAA AATGAAGTTC CGCTTTCTGG GAAAACTAAT GGCTAAGGCA 
ATCATGGATT TTAGACTGGT GGATCTTCCT CTTGGACTTC CTTTTTATAA ATGGATGTTA 
CGCCAGGAAA CTTCTTTGAC GTCACATGAC TTGTTCAGTA TTGATCCAGT AGTAGCCAAA 
TCAATATATC ACCTTGAAGA TATTGTAAGA CAAAAGAAAA GACTTGAGCA GGACAAAACA 
CAGACCAAAG AAAGTCTACA GTATGCATTG GAAACCCTGA CTATGAATGG CTGCTCAGTG 
GAGGATCTAG GGCTGGATTT CACACTTCCT GGATTTCCTA ACATCGAGCT GAAAAAAGGG 
GGAAAAGATA CACCAGTCAC CATCCACAAC TTGGAGGAGT ACCTCAGATT GGTTATATTC 
TGGGCACTTA ACGAAGGTGT TGCCAGACAG TTTGATTCTT TCAGAGATGG ATTTGAATCA 
GTCTTCCCCC TCAGTCATCT TCAATACTTT TATCCTGAGG AGTTGGAGCA ACTCTTATGT 
GGCAGCAAAA CAGACACATG GGATGCCAAG ACCTTAATGG AATGTTGCAG GCCAGATCAC 
GGTTATACAC ATGACAGTCG AGCAGTGAAG TATCTCTTTG AAATTCTCAG TAGTTTTGAT 
AGTGAGCAGC AGAGACTGTT TCTTCAGTTT GTGACCGGTA GTCCCAGGCT GCCAGTAGGA 
GGCTTTCGAA GTTTGAATCC TCCATTGACA ATTGTACGGA AGACATTTGA GTCTACAGAG 
AATCCAGATG ACTTCTTGCC CTCAGTAATG ACTTGTGTGA ACTATCTCAA ATTGCCGGAC 
TATTCAACTA TTGAGATAAT GCGTGAAAAA CTCTTGATAG CTGCAAGAGA AGGGCAGCAG 
TCATTCCATC TTTCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TRIP12 ( XM_015276966.1 ) cDNA ORF clone, Gallus gallus(chicken) -> XP_015132452.1 Gallus gallus thyroid hormone receptor interactor 12 (TRIP12), transcript variant X4, mRNA.

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CloneID	OGa12502
Clone ID Related Accession (Same CDS sequence)	XM_015276964.1 , XM_015276968.2 , XM_015276967.1 , XM_015276968.1 , XM_004937064.3 , XM_015276964.2 , XM_015276966.2 , XM_004937064.2 , XM_015276966.1 , XM_015276967.2 , XM_015276965.1
Accession Version	XM_015276966.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6198bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-03
Organism	Gallus gallus(chicken)
Product	E3 ubiquitin-protein ligase TRIP12 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_001471743.3) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Gallus gallus Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCCAACC GGCCTAATAA CAATCCAGGG GGGTCACTGC GACGTTCACA GAGGAACACT 
GCTGGGGCCC AGCCACAAGA CGACGCAGCA GGAGGAAGGT CACATTTAGG GCAGGCAAAA 
CATAAGGCAC ATAGCCCTCC TGAGAGTAGA AAATCTATTT CAAAGACACC CAAAGTGCAG 
TCTAATACTA CTTCTGAGCA GTCCAAGGGA CACTTTTCTA AAAGAGGCTG TAGTTCATCT 
GCTGTTTTAA TACCACAAGA AGATTCAGAA AGAGTCAGTA CTTCAGAAAA GCAAAAAACG 
GGGCAAGTGC CTAAGAAAGA CAATTCTCGA GGAGTTAAAC GCAGTGCTAG TCCAGATTAC 
AGCAGGACCA ATTCTCCTAG CTCTGCTAAA AAACCTAAAG CACTTCAACA CGCCGAAACT 
TCCTCGGAAA GTAACAAGCC ACATACTAAA TCCAAGAGAA GACACTTAGA CCAAGAACAG 
CAGAAGTCTA CACAATTGCC ATCAACAAGC AAGGCTCACA CCAGAAAGGG TGGAGCTGCT 
GGTAGCTCCC GAAGTCAGAA AAGGAAAAGG ACAGAGAGTA CGTCTTGTAT AAAGAGTCGT 
TCAGCAGTTG AATCAACTGG AGCTGAAGAG AAGTCAGCAA AATCCTCCAA GCTGGCTTCA 
AAATCGGTGA CCTCAGCCAA AGCTGGGTGT AGCACCATCA CTGATTCTTC TTCTTCAGCT 
TCTACATCCT CTTCCTCTTC TGCTGTTGCC TCTGCTTCTT CCACTGTTCC TCAGGGTGCC 
AGAGTGAAAC AGGGTAAGGA CCAGAATAAG GCTAGACGTT CACGTTCTGC GTCCAGCCCT 
AGTCCAAGAA GGAGCAGCAG GGACAAAGAA CAGAGCAAAA CAGGTGGCTC TTCAAAGTTT 
GACTGGGCTG CTCGATTCAG TCCAAAAGTT AGTCTCCCTA AAACAAAACT GTCTCTACCA 
GGCTCTTCTA AGTCAGAGAC CTCAAAACCT GGACCTTCAG GACTACAGGC TAAGCTAGCA 
AGCCTAAGAA AATCCACAAA GAAGCGCAGT GAATCACCAC CTGCTGAGCT CCCCAGTTTA 
CGGCGGAGCA CACGGCAAAA GACCACGGGC TCCTGTGCGA GTACCAGTCG GCGGGGCTCT 
GGCCTGGGCA AAAGAGGAGC AGCTGAAGCT CGCCGACAGG AGAAGATGGC TGATCCTGAC 
AACAACCAGG ATGGAGTTAA CTCTTCAGCT GCACGTACAG ATGAGACTCC CCAGGGAGCT 
GCAGCTTCTA GCTCTGTTGC TGGGGCTGTA GGTATGACAA CATCTGGAGA AAGTGAGTCA 
GATGACTCTG AGATGGGAAG ACTACAAGCT CTGTTAGAGG CTAGGGGTCT TCCTCCTCAC 
CTGTTTGGCC CTCTTGGTCC TCGGATGTCG CAGCTCTTCC ACAGGACAAT TGGAAGTGGA 
GCTAGTTCTA AAGCCCAGCA GCTTTTACAA GGTCTCCAAG CCACTGATGA AAGTCAGCAA 
CTACAGGCAG TTATTGAGAT GTGCCAGCTG TTGGTCATGG GAAATGAAGA AACACTGGGA 
GGATTTCCAG TCAAGAGTGT TGTGCCAGCT TTGATAACAT TGCTGCAGAT GGAGCATAAC 
TTTGACATCA TGAACCATGC TTGTCGAGCC TTAACATACA TGATGGAAGC ACTTCCCAGA 
TCATCTGCTG TAGTGGTAGA TGCAATTCCT GTCTTCTTGG AAAAGCTGCA AGTTATTCAG 
TGCATTGATG TGGCAGAGCA GGCACTGACA GCCCTAGAGA TGCTATCACG CAGGCATAGT 
AAAGCCATTC TGCAGGCAGG TGGGTTGGCA GACTGCTTGC TGTATCTGGA ATTCTTCAGT 
ATAAATGCGC AGAGGAATGC ACTAGCTATT GCTGCCAATT GCTGCCAGAG TATAACACCT 
GATGAGTTTC ACTTTGTGGC AGACTCTTTG CCACTGCTTA CACAAAGGTT AACCCATCAG 
GACAAGAAGT CTGTTGAAAG CACTTGTCTC TGTTTTGCAC GGCTGGTAGA CAATTTCCAG 
CATGAGGAGA ACTTGCTGCA GCAGGTTGCT TCCAAGGACT TGTTAACAAA CATCCAGCAA 
CTCTTGGTAG TGACACCTCC TATCCTGAGC TCAGGAATGT TCATCATGGT GGTGCGCATG 
TTTTCCTTAA TGTGTTCCAA TTGTCCAACG CTTGCAGTCC AACTTATGAA GCAAAACATT 
GCAGAAACAC TTCACTTTCT CCTTTGTGGA GCCTCAAATG GGAGTTGTCA AGAACAAATT 
GACCTTGTTC CACGAAGTCC TCAAGAACTC TATGAGCTTA CTTCTCTTAT CTGTGAACTG 
ATGCCTTGTC TGCCAAAAGA GGGGATATTT GCTGTTGATA CTATGCTAAA GAAAGGAAAT 
GCACAAAATA CAGATGGTGC GATATGGCAA TGGCGAGATG ACAGGGGTCT CTGGCATCCC 
TATAACAGGA TTGATAGTCG AATAATAGAG GCAGCTCATC AGGTTGGTGA GGATGAGATA 
AGCCTGTCGA CACTTGGACG TGTCTATACT ATTGATTTTA ATTCTATGCA GCAAATAAAT 
GAGGATACTG GAACAGCACG TGCCATTCAG CGGAAACCAA ACCCTTTAGC CAATACAAAC 
ACTAGTGGAC ATTCAGAATT GAAGAAGGAT GATGCTCGAG CACAGCTAAT GAAAGAAGAC 
CCAGAATTGG CAAAATCCTT TATCAAAACA CTGTTTGGTG TTCTTTATGA AGTGTATAGT 
TCTTCAGCTG GACCTGCTGT TAGACACAAG TGCCTTAGAG CAATACTTAG AATAATTTAT 
TTTGCTGATG CTGAACTCCT GAAGGATGTG CTGAAAAACC ATGCTGTGTC AAGTCACATT 
GCCTCCATGC TGTCAAGTCA AGACCTTAAG ATAGTAGTTG GAGCCCTGCA GATGGCAGAA 
ATCTTAATGC AGAAGCTACC TGACATTTTT AGTGTTTACT TCAGAAGAGA AGGAGTGATG 
CATCAAGTGA AAAACTTAGC GGAGTCTGAG GCTTTGCTAA CGAGCCCACC AAAAGTATGC 
ACTAATGGAT CAGGAACACT GGGTACCACT ACAACTATAA GTACTGGAAC AGCCACTGCT 
GCCACTAATG CAGCTGCAGA TTTGGGCTCT CCCAGCTTAC AACACAGCCG GGAGGATTCT 
TTAGATCTGA GCCCACAGGG ACGACTGAGT GATGTTCTGA AGAGAAAAAG ACTGCCAAAA 
CGAGGGCCAA GGAGACCAAA ATATTCTCCC CCAAGAGATG ATGACAAAGT AGACAATCAA 
GCAAAAAGCC CCACGACTAC TCAGTCTCCT AAATCTTCTT TCTTAGCAAG TTTAAATCCC 
AAAACATGGG GAAGATTAAG TACACAGTCT AACAGTAATA ACATTGAACC AGCAAGAACA 
GCAGGTGTAA GTGGTCTTGC AAGAGCTGCT TCTAAGGATA CAATTTCCAA TAACAGAGAA 
AAAATTAAGG GTTGGATTAA GGAGCAAGCC CATAAATTTG TAGACCGTTA TTTTAGTTCG 
GAAAACATGG ATGGAAGCAA TCCTGCACTA AATGTATTAC AGAGACTTTG CACTGCAACT 
GAACAACTTA ACCTCCAGGT GGATGGTGGA ACAGAGTGCC TTGTAGAAAT CCGTAGCATT 
GTCTCGGAGT CTGACGTCTC CTCATTTGAA ATCCAGCATA GTGGATTTGT GAAACAACTG 
CTGCTTTATT TGACATCTAA AAGTGAGAAA GATGCTGTAA GCAGGGATAT CAGATTGAAA 
AGATTTCTTC ATGTATTTTT CTCCTCTCCA CTTCCTGGAG AAGAACCCCT TGGAAGATTA 
GAGCCATTAG AAAATGCACC TTTGTTGGCA TTAGTCCATA AAATGAACAA TTGCCTCAGT 
CAGATGGAGC AGTTTCCAGT GAAAGTGCAT GACTTCCCCA GTGGAAATGG AACAGGGAGC 
AGTTTTTCTC TTAACAGAGG ATCCCAAGCT TTAAAATTCT TCAATACGCA TCAGTTAAAA 
TGCCAGCTGC AAAGACATCC AGACTGTGCT AATGTGAAAC AGTGGAAGGG TGGACCTGTG 
AAGATTGATC CTCTGGCTTT GGTTCAAGCC ATTGAAAGAT ATCTTGTTGT TAGAGGTTAT 
GGAAGAGTTA GAGAAGATGA TGAGGATAGT GATGATGATG GGTCAGATGA AGAAATAGAT 
GAATCCTTGG CTGCTCAATT CTTAAATTCA GGGAATGTGA GACATAGATT GCAGTTTTAC 
ATTGGGGATC ACTTGCTACC ATATAATATG ACTGTATATC AAGCAGTTAA GCTGTACAGT 
TTGCAAGCTG AGGAGGAGAG GGAATCGACT GATGATGAAA GCAATCCATT AGGAAGAGCT 
GGGATTTGGA CAAAAACACA TACCATTTGG TACAAACCTG TACGGGAGGA TGAAGATGGC 
AACAAAGACT GTGTTGGTGG TAAAAGAGGA AGAGCTCAAA CTGCTCCCAC AAAAACCTCA 
CCTAGGAATT CTAAAAAGCA TGATGAGTTG TGGCACGATG GTGTATGCCC TTCAGTATTG 
AATCCTTTAG AAGTTTACCT CATATCTACT CCTCCTGAAA ACATAACCTT TGAAGATCCT 
TCATTAGATG TTATTCTTCT TTTGAGAGTT TTACATGCTA TCAGTCGATA CTGGTATTAT 
TTGTATGATA ATGCAATATG CAAGGAGATT ATTCCAACTT CGGAATTTAT CAATAGTAAA 
CTGACAGCAA AAGCAAACAG ACAGCTTCAA GATCCACTGG TAATTATGAC AGGAAACATA 
CCGACTTGGC TGACAGAGCT TGGAAAAACA TGCCCATTTT TCTTTCCATT TGATACCCGG 
CAAATGCTGT TTTATGTAAC TGCTTTTGAT CGTGATCGAG CCATGCAAAG ACTACTGGAT 
ACTAATCCAG AAATCAATCA ATCAGATTCT CAGGATAGCA GAGTGGCACC ACGACTGGAC 
AGGAAAAAAC GCACTGTGAA CAGAGATGAG CTGTTGAAAC AGGCAGAATC TGTGATGCAG 
GATCTAGGCA GTTCAAGAGC CATGTTGGAA ATCCAGTATG AGAATGAAGT TGGCACAGGC 
CTGGGCCCTA CACTAGAGTT CTATGCACTT GTATCTCAGG AACTACAGAG AGCAGACTTG 
GGCCTTTGGA GGGGAGAAGA AGTAACTTTA GCCAATCCAA AAGGAAGCCA AGAAGGTACC 
AAGTATATTC ATAATCTCCA AGGCCTTTTT GCACTTCCTT TTGGTAGAAC AGCCAAGCCT 
GCTCACATTG CAAAAGTTAA AATGAAGTTC CGCTTTCTGG GAAAACTAAT GGCTAAGGCA 
ATCATGGATT TTAGACTGGT GGATCTTCCT CTTGGACTTC CTTTTTATAA ATGGATGTTA 
CGCCAGGAAA CTTCTTTGAC GTCACATGAC TTGTTCAGTA TTGATCCAGT AGTAGCCAAA 
TCAATATATC ACCTTGAAGA TATTGTAAGA CAAAAGAAAA GACTTGAGCA GGACAAAACA 
CAGACCAAAG AAAGTCTACA GTATGCATTG GAAACCCTGA CTATGAATGG CTGCTCAGTG 
GAGGATCTAG GGCTGGATTT CACACTTCCT GGATTTCCTA ACATCGAGCT GAAAAAAGGG 
GGAAAAGATA CACCAGTCAC CATCCACAAC TTGGAGGAGT ACCTCAGATT GGTTATATTC 
TGGGCACTTA ACGAAGGTGT TGCCAGACAG TTTGATTCTT TCAGAGATGG ATTTGAATCA 
GTCTTCCCCC TCAGTCATCT TCAATACTTT TATCCTGAGG AGTTGGAGCA ACTCTTATGT 
GGCAGCAAAA CAGACACATG GGATGCCAAG ACCTTAATGG AATGTTGCAG GCCAGATCAC 
GGTTATACAC ATGACAGTCG AGCAGTGAAG TATCTCTTTG AAATTCTCAG TAGTTTTGAT 
AGTGAGCAGC AGAGACTGTT TCTTCAGTTT GTGACCGGTA GTCCCAGGCT GCCAGTAGGA 
GGCTTTCGAA GTTTGAATCC TCCATTGACA ATTGTACGGA AGACATTTGA GTCTACAGAG 
AATCCAGATG ACTTCTTGCC CTCAGTAATG ACTTGTGTGA ACTATCTCAA ATTGCCGGAC 
TATTCAACTA TTGAGATAAT GCGTGAAAAA CTCTTGATAG CTGCAAGAGA AGGGCAGCAG 
TCATTCCATC TTTCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015132452.1
CDS	134..6331
Translation	MSNRPNNNPGGSLRRSQRNTAGAQPQDDAAGGRSHLGQAKHKAHSPPESRKSISKTPKVQSNTTSEQSKGHFSKRGCSSSAVLIPQEDSERVSTSEKQKTGQVPKKDNSRGVKRSASPDYSRTNSPSSAKKPKALQHAETSSESNKPHTKSKRRHLDQEQQKSTQLPSTSKAHTRKGGAAGSSRSQKRKRTESTSCIKSRSAVESTGAEEKSAKSSKLASKSVTSAKAGCSTITDSSSSASTSSSSSAVASASSTVPQGARVKQGKDQNKARRSRSASSPSPRRSSRDKEQSKTGGSSKFDWAARFSPKVSLPKTKLSLPGSSKSETSKPGPSGLQAKLASLRKSTKKRSESPPAELPSLRRSTRQKTTGSCASTSRRGSGLGKRGAAEARRQEKMADPDNNQDGVNSSAARTDETPQGAAASSSVAGAVGMTTSGESESDDSEMGRLQALLEARGLPPHLFGPLGPRMSQLFHRTIGSGASSKAQQLLQGLQATDESQQLQAVIEMCQLLVMGNEETLGGFPVKSVVPALITLLQMEHNFDIMNHACRALTYMMEALPRSSAVVVDAIPVFLEKLQVIQCIDVAEQALTALEMLSRRHSKAILQAGGLADCLLYLEFFSINAQRNALAIAANCCQSITPDEFHFVADSLPLLTQRLTHQDKKSVESTCLCFARLVDNFQHEENLLQQVASKDLLTNIQQLLVVTPPILSSGMFIMVVRMFSLMCSNCPTLAVQLMKQNIAETLHFLLCGASNGSCQEQIDLVPRSPQELYELTSLICELMPCLPKEGIFAVDTMLKKGNAQNTDGAIWQWRDDRGLWHPYNRIDSRIIEAAHQVGEDEISLSTLGRVYTIDFNSMQQINEDTGTARAIQRKPNPLANTNTSGHSELKKDDARAQLMKEDPELAKSFIKTLFGVLYEVYSSSAGPAVRHKCLRAILRIIYFADAELLKDVLKNHAVSSHIASMLSSQDLKIVVGALQMAEILMQKLPDIFSVYFRREGVMHQVKNLAESEALLTSPPKVCTNGSGTLGTTTTISTGTATAATNAAADLGSPSLQHSREDSLDLSPQGRLSDVLKRKRLPKRGPRRPKYSPPRDDDKVDNQAKSPTTTQSPKSSFLASLNPKTWGRLSTQSNSNNIEPARTAGVSGLARAASKDTISNNREKIKGWIKEQAHKFVDRYFSSENMDGSNPALNVLQRLCTATEQLNLQVDGGTECLVEIRSIVSESDVSSFEIQHSGFVKQLLLYLTSKSEKDAVSRDIRLKRFLHVFFSSPLPGEEPLGRLEPLENAPLLALVHKMNNCLSQMEQFPVKVHDFPSGNGTGSSFSLNRGSQALKFFNTHQLKCQLQRHPDCANVKQWKGGPVKIDPLALVQAIERYLVVRGYGRVREDDEDSDDDGSDEEIDESLAAQFLNSGNVRHRLQFYIGDHLLPYNMTVYQAVKLYSLQAEEERESTDDESNPLGRAGIWTKTHTIWYKPVREDEDGNKDCVGGKRGRAQTAPTKTSPRNSKKHDELWHDGVCPSVLNPLEVYLISTPPENITFEDPSLDVILLLRVLHAISRYWYYLYDNAICKEIIPTSEFINSKLTAKANRQLQDPLVIMTGNIPTWLTELGKTCPFFFPFDTRQMLFYVTAFDRDRAMQRLLDTNPEINQSDSQDSRVAPRLDRKKRTVNRDELLKQAESVMQDLGSSRAMLEIQYENEVGTGLGPTLEFYALVSQELQRADLGLWRGEEVTLANPKGSQEGTKYIHNLQGLFALPFGRTAKPAHIAKVKMKFRFLGKLMAKAIMDFRLVDLPLGLPFYKWMLRQETSLTSHDLFSIDPVVAKSIYHLEDIVRQKKRLEQDKTQTKESLQYALETLTMNGCSVEDLGLDFTLPGFPNIELKKGGKDTPVTIHNLEEYLRLVIFWALNEGVARQFDSFRDGFESVFPLSHLQYFYPEELEQLLCGSKTDTWDAKTLMECCRPDHGYTHDSRAVKYLFEILSSFDSEQQRLFLQFVTGSPRLPVGGFRSLNPPLTIVRKTFESTENPDDFLPSVMTCVNYLKLPDYSTIEIMREKLLIAAREGQQSFHLS

Target ORF information:

RefSeq Version	XM_015276966.1
Organism	Gallus gallus(chicken)
Definition	Gallus gallus thyroid hormone receptor interactor 12 (TRIP12), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015276966.1

ORF Insert Sequence:

ATGTCCAACC GGCCTAATAA CAATCCAGGG GGGTCACTGC GACGTTCACA GAGGAACACT 
GCTGGGGCCC AGCCACAAGA CGACGCAGCA GGAGGAAGGT CACATTTAGG GCAGGCAAAA 
CATAAGGCAC ATAGCCCTCC TGAGAGTAGA AAATCTATTT CAAAGACACC CAAAGTGCAG 
TCTAATACTA CTTCTGAGCA GTCCAAGGGA CACTTTTCTA AAAGAGGCTG TAGTTCATCT 
GCTGTTTTAA TACCACAAGA AGATTCAGAA AGAGTCAGTA CTTCAGAAAA GCAAAAAACG 
GGGCAAGTGC CTAAGAAAGA CAATTCTCGA GGAGTTAAAC GCAGTGCTAG TCCAGATTAC 
AGCAGGACCA ATTCTCCTAG CTCTGCTAAA AAACCTAAAG CACTTCAACA CGCCGAAACT 
TCCTCGGAAA GTAACAAGCC ACATACTAAA TCCAAGAGAA GACACTTAGA CCAAGAACAG 
CAGAAGTCTA CACAATTGCC ATCAACAAGC AAGGCTCACA CCAGAAAGGG TGGAGCTGCT 
GGTAGCTCCC GAAGTCAGAA AAGGAAAAGG ACAGAGAGTA CGTCTTGTAT AAAGAGTCGT 
TCAGCAGTTG AATCAACTGG AGCTGAAGAG AAGTCAGCAA AATCCTCCAA GCTGGCTTCA 
AAATCGGTGA CCTCAGCCAA AGCTGGGTGT AGCACCATCA CTGATTCTTC TTCTTCAGCT 
TCTACATCCT CTTCCTCTTC TGCTGTTGCC TCTGCTTCTT CCACTGTTCC TCAGGGTGCC 
AGAGTGAAAC AGGGTAAGGA CCAGAATAAG GCTAGACGTT CACGTTCTGC GTCCAGCCCT 
AGTCCAAGAA GGAGCAGCAG GGACAAAGAA CAGAGCAAAA CAGGTGGCTC TTCAAAGTTT 
GACTGGGCTG CTCGATTCAG TCCAAAAGTT AGTCTCCCTA AAACAAAACT GTCTCTACCA 
GGCTCTTCTA AGTCAGAGAC CTCAAAACCT GGACCTTCAG GACTACAGGC TAAGCTAGCA 
AGCCTAAGAA AATCCACAAA GAAGCGCAGT GAATCACCAC CTGCTGAGCT CCCCAGTTTA 
CGGCGGAGCA CACGGCAAAA GACCACGGGC TCCTGTGCGA GTACCAGTCG GCGGGGCTCT 
GGCCTGGGCA AAAGAGGAGC AGCTGAAGCT CGCCGACAGG AGAAGATGGC TGATCCTGAC 
AACAACCAGG ATGGAGTTAA CTCTTCAGCT GCACGTACAG ATGAGACTCC CCAGGGAGCT 
GCAGCTTCTA GCTCTGTTGC TGGGGCTGTA GGTATGACAA CATCTGGAGA AAGTGAGTCA 
GATGACTCTG AGATGGGAAG ACTACAAGCT CTGTTAGAGG CTAGGGGTCT TCCTCCTCAC 
CTGTTTGGCC CTCTTGGTCC TCGGATGTCG CAGCTCTTCC ACAGGACAAT TGGAAGTGGA 
GCTAGTTCTA AAGCCCAGCA GCTTTTACAA GGTCTCCAAG CCACTGATGA AAGTCAGCAA 
CTACAGGCAG TTATTGAGAT GTGCCAGCTG TTGGTCATGG GAAATGAAGA AACACTGGGA 
GGATTTCCAG TCAAGAGTGT TGTGCCAGCT TTGATAACAT TGCTGCAGAT GGAGCATAAC 
TTTGACATCA TGAACCATGC TTGTCGAGCC TTAACATACA TGATGGAAGC ACTTCCCAGA 
TCATCTGCTG TAGTGGTAGA TGCAATTCCT GTCTTCTTGG AAAAGCTGCA AGTTATTCAG 
TGCATTGATG TGGCAGAGCA GGCACTGACA GCCCTAGAGA TGCTATCACG CAGGCATAGT 
AAAGCCATTC TGCAGGCAGG TGGGTTGGCA GACTGCTTGC TGTATCTGGA ATTCTTCAGT 
ATAAATGCGC AGAGGAATGC ACTAGCTATT GCTGCCAATT GCTGCCAGAG TATAACACCT 
GATGAGTTTC ACTTTGTGGC AGACTCTTTG CCACTGCTTA CACAAAGGTT AACCCATCAG 
GACAAGAAGT CTGTTGAAAG CACTTGTCTC TGTTTTGCAC GGCTGGTAGA CAATTTCCAG 
CATGAGGAGA ACTTGCTGCA GCAGGTTGCT TCCAAGGACT TGTTAACAAA CATCCAGCAA 
CTCTTGGTAG TGACACCTCC TATCCTGAGC TCAGGAATGT TCATCATGGT GGTGCGCATG 
TTTTCCTTAA TGTGTTCCAA TTGTCCAACG CTTGCAGTCC AACTTATGAA GCAAAACATT 
GCAGAAACAC TTCACTTTCT CCTTTGTGGA GCCTCAAATG GGAGTTGTCA AGAACAAATT 
GACCTTGTTC CACGAAGTCC TCAAGAACTC TATGAGCTTA CTTCTCTTAT CTGTGAACTG 
ATGCCTTGTC TGCCAAAAGA GGGGATATTT GCTGTTGATA CTATGCTAAA GAAAGGAAAT 
GCACAAAATA CAGATGGTGC GATATGGCAA TGGCGAGATG ACAGGGGTCT CTGGCATCCC 
TATAACAGGA TTGATAGTCG AATAATAGAG GCAGCTCATC AGGTTGGTGA GGATGAGATA 
AGCCTGTCGA CACTTGGACG TGTCTATACT ATTGATTTTA ATTCTATGCA GCAAATAAAT 
GAGGATACTG GAACAGCACG TGCCATTCAG CGGAAACCAA ACCCTTTAGC CAATACAAAC 
ACTAGTGGAC ATTCAGAATT GAAGAAGGAT GATGCTCGAG CACAGCTAAT GAAAGAAGAC 
CCAGAATTGG CAAAATCCTT TATCAAAACA CTGTTTGGTG TTCTTTATGA AGTGTATAGT 
TCTTCAGCTG GACCTGCTGT TAGACACAAG TGCCTTAGAG CAATACTTAG AATAATTTAT 
TTTGCTGATG CTGAACTCCT GAAGGATGTG CTGAAAAACC ATGCTGTGTC AAGTCACATT 
GCCTCCATGC TGTCAAGTCA AGACCTTAAG ATAGTAGTTG GAGCCCTGCA GATGGCAGAA 
ATCTTAATGC AGAAGCTACC TGACATTTTT AGTGTTTACT TCAGAAGAGA AGGAGTGATG 
CATCAAGTGA AAAACTTAGC GGAGTCTGAG GCTTTGCTAA CGAGCCCACC AAAAGTATGC 
ACTAATGGAT CAGGAACACT GGGTACCACT ACAACTATAA GTACTGGAAC AGCCACTGCT 
GCCACTAATG CAGCTGCAGA TTTGGGCTCT CCCAGCTTAC AACACAGCCG GGAGGATTCT 
TTAGATCTGA GCCCACAGGG ACGACTGAGT GATGTTCTGA AGAGAAAAAG ACTGCCAAAA 
CGAGGGCCAA GGAGACCAAA ATATTCTCCC CCAAGAGATG ATGACAAAGT AGACAATCAA 
GCAAAAAGCC CCACGACTAC TCAGTCTCCT AAATCTTCTT TCTTAGCAAG TTTAAATCCC 
AAAACATGGG GAAGATTAAG TACACAGTCT AACAGTAATA ACATTGAACC AGCAAGAACA 
GCAGGTGTAA GTGGTCTTGC AAGAGCTGCT TCTAAGGATA CAATTTCCAA TAACAGAGAA 
AAAATTAAGG GTTGGATTAA GGAGCAAGCC CATAAATTTG TAGACCGTTA TTTTAGTTCG 
GAAAACATGG ATGGAAGCAA TCCTGCACTA AATGTATTAC AGAGACTTTG CACTGCAACT 
GAACAACTTA ACCTCCAGGT GGATGGTGGA ACAGAGTGCC TTGTAGAAAT CCGTAGCATT 
GTCTCGGAGT CTGACGTCTC CTCATTTGAA ATCCAGCATA GTGGATTTGT GAAACAACTG 
CTGCTTTATT TGACATCTAA AAGTGAGAAA GATGCTGTAA GCAGGGATAT CAGATTGAAA 
AGATTTCTTC ATGTATTTTT CTCCTCTCCA CTTCCTGGAG AAGAACCCCT TGGAAGATTA 
GAGCCATTAG AAAATGCACC TTTGTTGGCA TTAGTCCATA AAATGAACAA TTGCCTCAGT 
CAGATGGAGC AGTTTCCAGT GAAAGTGCAT GACTTCCCCA GTGGAAATGG AACAGGGAGC 
AGTTTTTCTC TTAACAGAGG ATCCCAAGCT TTAAAATTCT TCAATACGCA TCAGTTAAAA 
TGCCAGCTGC AAAGACATCC AGACTGTGCT AATGTGAAAC AGTGGAAGGG TGGACCTGTG 
AAGATTGATC CTCTGGCTTT GGTTCAAGCC ATTGAAAGAT ATCTTGTTGT TAGAGGTTAT 
GGAAGAGTTA GAGAAGATGA TGAGGATAGT GATGATGATG GGTCAGATGA AGAAATAGAT 
GAATCCTTGG CTGCTCAATT CTTAAATTCA GGGAATGTGA GACATAGATT GCAGTTTTAC 
ATTGGGGATC ACTTGCTACC ATATAATATG ACTGTATATC AAGCAGTTAA GCTGTACAGT 
TTGCAAGCTG AGGAGGAGAG GGAATCGACT GATGATGAAA GCAATCCATT AGGAAGAGCT 
GGGATTTGGA CAAAAACACA TACCATTTGG TACAAACCTG TACGGGAGGA TGAAGATGGC 
AACAAAGACT GTGTTGGTGG TAAAAGAGGA AGAGCTCAAA CTGCTCCCAC AAAAACCTCA 
CCTAGGAATT CTAAAAAGCA TGATGAGTTG TGGCACGATG GTGTATGCCC TTCAGTATTG 
AATCCTTTAG AAGTTTACCT CATATCTACT CCTCCTGAAA ACATAACCTT TGAAGATCCT 
TCATTAGATG TTATTCTTCT TTTGAGAGTT TTACATGCTA TCAGTCGATA CTGGTATTAT 
TTGTATGATA ATGCAATATG CAAGGAGATT ATTCCAACTT CGGAATTTAT CAATAGTAAA 
CTGACAGCAA AAGCAAACAG ACAGCTTCAA GATCCACTGG TAATTATGAC AGGAAACATA 
CCGACTTGGC TGACAGAGCT TGGAAAAACA TGCCCATTTT TCTTTCCATT TGATACCCGG 
CAAATGCTGT TTTATGTAAC TGCTTTTGAT CGTGATCGAG CCATGCAAAG ACTACTGGAT 
ACTAATCCAG AAATCAATCA ATCAGATTCT CAGGATAGCA GAGTGGCACC ACGACTGGAC 
AGGAAAAAAC GCACTGTGAA CAGAGATGAG CTGTTGAAAC AGGCAGAATC TGTGATGCAG 
GATCTAGGCA GTTCAAGAGC CATGTTGGAA ATCCAGTATG AGAATGAAGT TGGCACAGGC 
CTGGGCCCTA CACTAGAGTT CTATGCACTT GTATCTCAGG AACTACAGAG AGCAGACTTG 
GGCCTTTGGA GGGGAGAAGA AGTAACTTTA GCCAATCCAA AAGGAAGCCA AGAAGGTACC 
AAGTATATTC ATAATCTCCA AGGCCTTTTT GCACTTCCTT TTGGTAGAAC AGCCAAGCCT 
GCTCACATTG CAAAAGTTAA AATGAAGTTC CGCTTTCTGG GAAAACTAAT GGCTAAGGCA 
ATCATGGATT TTAGACTGGT GGATCTTCCT CTTGGACTTC CTTTTTATAA ATGGATGTTA 
CGCCAGGAAA CTTCTTTGAC GTCACATGAC TTGTTCAGTA TTGATCCAGT AGTAGCCAAA 
TCAATATATC ACCTTGAAGA TATTGTAAGA CAAAAGAAAA GACTTGAGCA GGACAAAACA 
CAGACCAAAG AAAGTCTACA GTATGCATTG GAAACCCTGA CTATGAATGG CTGCTCAGTG 
GAGGATCTAG GGCTGGATTT CACACTTCCT GGATTTCCTA ACATCGAGCT GAAAAAAGGG 
GGAAAAGATA CACCAGTCAC CATCCACAAC TTGGAGGAGT ACCTCAGATT GGTTATATTC 
TGGGCACTTA ACGAAGGTGT TGCCAGACAG TTTGATTCTT TCAGAGATGG ATTTGAATCA 
GTCTTCCCCC TCAGTCATCT TCAATACTTT TATCCTGAGG AGTTGGAGCA ACTCTTATGT 
GGCAGCAAAA CAGACACATG GGATGCCAAG ACCTTAATGG AATGTTGCAG GCCAGATCAC 
GGTTATACAC ATGACAGTCG AGCAGTGAAG TATCTCTTTG AAATTCTCAG TAGTTTTGAT 
AGTGAGCAGC AGAGACTGTT TCTTCAGTTT GTGACCGGTA GTCCCAGGCT GCCAGTAGGA 
GGCTTTCGAA GTTTGAATCC TCCATTGACA ATTGTACGGA AGACATTTGA GTCTACAGAG 
AATCCAGATG ACTTCTTGCC CTCAGTAATG ACTTGTGTGA ACTATCTCAA ATTGCCGGAC 
TATTCAACTA TTGAGATAAT GCGTGAAAAA CTCTTGATAG CTGCAAGAGA AGGGCAGCAG 
TCATTCCATC TTTCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TRIP12 ( XM_015276967.2 ) cDNA ORF clone, Gallus gallus(chicken) -> XP_015132453.1 Gallus gallus thyroid hormone receptor interactor 12 (TRIP12), transcript variant X4, mRNA.

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CloneID	OGa12502
Clone ID Related Accession (Same CDS sequence)	XM_015276964.1 , XM_015276968.2 , XM_015276967.1 , XM_015276968.1 , XM_004937064.3 , XM_015276964.2 , XM_015276966.2 , XM_004937064.2 , XM_015276966.1 , XM_015276967.2 , XM_015276965.1
Accession Version	XM_015276967.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6198bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-16
Organism	Gallus gallus(chicken)
Product	E3 ubiquitin-protein ligase TRIP12 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006096.5) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On May 17, 2018 this sequence version replaced XM_015276967.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Gallus gallus Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCCAACC GGCCTAATAA CAATCCAGGG GGGTCACTGC GACGTTCACA GAGGAACACT 
GCTGGGGCCC AGCCACAAGA CGACGCAGCA GGAGGAAGGT CACATTTAGG GCAGGCAAAA 
CATAAGGCAC ATAGCCCTCC TGAGAGTAGA AAATCTATTT CAAAGACACC CAAAGTGCAG 
TCTAATACTA CTTCTGAGCA GTCCAAGGGA CACTTTTCTA AAAGAGGCTG TAGTTCATCT 
GCTGTTTTAA TACCACAAGA AGATTCAGAA AGAGTCAGTA CTTCAGAAAA GCAAAAAACG 
GGGCAAGTGC CTAAGAAAGA CAATTCTCGA GGAGTTAAAC GCAGTGCTAG TCCAGATTAC 
AGCAGGACCA ATTCTCCTAG CTCTGCTAAA AAACCTAAAG CACTTCAACA CGCCGAAACT 
TCCTCGGAAA GTAACAAGCC ACATACTAAA TCCAAGAGAA GACACTTAGA CCAAGAACAG 
CAGAAGTCTA CACAATTGCC ATCAACAAGC AAGGCTCACA CCAGAAAGGG TGGAGCTGCT 
GGTAGCTCCC GAAGTCAGAA AAGGAAAAGG ACAGAGAGTA CGTCTTGTAT AAAGAGTCGT 
TCAGCAGTTG AATCAACTGG AGCTGAAGAG AAGTCAGCAA AATCCTCCAA GCTGGCTTCA 
AAATCGGTGA CCTCAGCCAA AGCTGGGTGT AGCACCATCA CTGATTCTTC TTCTTCAGCT 
TCTACATCCT CTTCCTCTTC TGCTGTTGCC TCTGCTTCTT CCACTGTTCC TCAGGGTGCC 
AGAGTGAAAC AGGGTAAGGA CCAGAATAAG GCTAGACGTT CACGTTCTGC GTCCAGCCCT 
AGTCCAAGAA GGAGCAGCAG GGACAAAGAA CAGAGCAAAA CAGGTGGCTC TTCAAAGTTT 
GACTGGGCTG CTCGATTCAG TCCAAAAGTT AGTCTCCCTA AAACAAAACT GTCTCTACCA 
GGCTCTTCTA AGTCAGAGAC CTCAAAACCT GGACCTTCAG GACTACAGGC TAAGCTAGCA 
AGCCTAAGAA AATCCACAAA GAAGCGCAGT GAATCACCAC CTGCTGAGCT CCCCAGTTTA 
CGGCGGAGCA CACGGCAAAA GACCACGGGC TCCTGTGCGA GTACCAGTCG GCGGGGCTCT 
GGCCTGGGCA AAAGAGGAGC AGCTGAAGCT CGCCGACAGG AGAAGATGGC TGATCCTGAC 
AACAACCAGG ATGGAGTTAA CTCTTCAGCT GCACGTACAG ATGAGACTCC CCAGGGAGCT 
GCAGCTTCTA GCTCTGTTGC TGGGGCTGTA GGTATGACAA CATCTGGAGA AAGTGAGTCA 
GATGACTCTG AGATGGGAAG ACTACAAGCT CTGTTAGAGG CTAGGGGTCT TCCTCCTCAC 
CTGTTTGGCC CTCTTGGTCC TCGGATGTCG CAGCTCTTCC ACAGGACAAT TGGAAGTGGA 
GCTAGTTCTA AAGCCCAGCA GCTTTTACAA GGTCTCCAAG CCACTGATGA AAGTCAGCAA 
CTACAGGCAG TTATTGAGAT GTGCCAGCTG TTGGTCATGG GAAATGAAGA AACACTGGGA 
GGATTTCCAG TCAAGAGTGT TGTGCCAGCT TTGATAACAT TGCTGCAGAT GGAGCATAAC 
TTTGACATCA TGAACCATGC TTGTCGAGCC TTAACATACA TGATGGAAGC ACTTCCCAGA 
TCATCTGCTG TAGTGGTAGA TGCAATTCCT GTCTTCTTGG AAAAGCTGCA AGTTATTCAG 
TGCATTGATG TGGCAGAGCA GGCACTGACA GCCCTAGAGA TGCTATCACG CAGGCATAGT 
AAAGCCATTC TGCAGGCAGG TGGGTTGGCA GACTGCTTGC TGTATCTGGA ATTCTTCAGT 
ATAAATGCGC AGAGGAATGC ACTAGCTATT GCTGCCAATT GCTGCCAGAG TATAACACCT 
GATGAGTTTC ACTTTGTGGC AGACTCTTTG CCACTGCTTA CACAAAGGTT AACCCATCAG 
GACAAGAAGT CTGTTGAAAG CACTTGTCTC TGTTTTGCAC GGCTGGTAGA CAATTTCCAG 
CATGAGGAGA ACTTGCTGCA GCAGGTTGCT TCCAAGGACT TGTTAACAAA CATCCAGCAA 
CTCTTGGTAG TGACACCTCC TATCCTGAGC TCAGGAATGT TCATCATGGT GGTGCGCATG 
TTTTCCTTAA TGTGTTCCAA TTGTCCAACG CTTGCAGTCC AACTTATGAA GCAAAACATT 
GCAGAAACAC TTCACTTTCT CCTTTGTGGA GCCTCAAATG GGAGTTGTCA AGAACAAATT 
GACCTTGTTC CACGAAGTCC TCAAGAACTC TATGAGCTTA CTTCTCTTAT CTGTGAACTG 
ATGCCTTGTC TGCCAAAAGA GGGGATATTT GCTGTTGATA CTATGCTAAA GAAAGGAAAT 
GCACAAAATA CAGATGGTGC GATATGGCAA TGGCGAGATG ACAGGGGTCT CTGGCATCCC 
TATAACAGGA TTGATAGTCG AATAATAGAG GCAGCTCATC AGGTTGGTGA GGATGAGATA 
AGCCTGTCGA CACTTGGACG TGTCTATACT ATTGATTTTA ATTCTATGCA GCAAATAAAT 
GAGGATACTG GAACAGCACG TGCCATTCAG CGGAAACCAA ACCCTTTAGC CAATACAAAC 
ACTAGTGGAC ATTCAGAATT GAAGAAGGAT GATGCTCGAG CACAGCTAAT GAAAGAAGAC 
CCAGAATTGG CAAAATCCTT TATCAAAACA CTGTTTGGTG TTCTTTATGA AGTGTATAGT 
TCTTCAGCTG GACCTGCTGT TAGACACAAG TGCCTTAGAG CAATACTTAG AATAATTTAT 
TTTGCTGATG CTGAACTCCT GAAGGATGTG CTGAAAAACC ATGCTGTGTC AAGTCACATT 
GCCTCCATGC TGTCAAGTCA AGACCTTAAG ATAGTAGTTG GAGCCCTGCA GATGGCAGAA 
ATCTTAATGC AGAAGCTACC TGACATTTTT AGTGTTTACT TCAGAAGAGA AGGAGTGATG 
CATCAAGTGA AAAACTTAGC GGAGTCTGAG GCTTTGCTAA CGAGCCCACC AAAAGTATGC 
ACTAATGGAT CAGGAACACT GGGTACCACT ACAACTATAA GTACTGGAAC AGCCACTGCT 
GCCACTAATG CAGCTGCAGA TTTGGGCTCT CCCAGCTTAC AACACAGCCG GGAGGATTCT 
TTAGATCTGA GCCCACAGGG ACGACTGAGT GATGTTCTGA AGAGAAAAAG ACTGCCAAAA 
CGAGGGCCAA GGAGACCAAA ATATTCTCCC CCAAGAGATG ATGACAAAGT AGACAATCAA 
GCAAAAAGCC CCACGACTAC TCAGTCTCCT AAATCTTCTT TCTTAGCAAG TTTAAATCCC 
AAAACATGGG GAAGATTAAG TACACAGTCT AACAGTAATA ACATTGAACC AGCAAGAACA 
GCAGGTGTAA GTGGTCTTGC AAGAGCTGCT TCTAAGGATA CAATTTCCAA TAACAGAGAA 
AAAATTAAGG GTTGGATTAA GGAGCAAGCC CATAAATTTG TAGACCGTTA TTTTAGTTCG 
GAAAACATGG ATGGAAGCAA TCCTGCACTA AATGTATTAC AGAGACTTTG CACTGCAACT 
GAACAACTTA ACCTCCAGGT GGATGGTGGA ACAGAGTGCC TTGTAGAAAT CCGTAGCATT 
GTCTCGGAGT CTGACGTCTC CTCATTTGAA ATCCAGCATA GTGGATTTGT GAAACAACTG 
CTGCTTTATT TGACATCTAA AAGTGAGAAA GATGCTGTAA GCAGGGATAT CAGATTGAAA 
AGATTTCTTC ATGTATTTTT CTCCTCTCCA CTTCCTGGAG AAGAACCCCT TGGAAGATTA 
GAGCCATTAG AAAATGCACC TTTGTTGGCA TTAGTCCATA AAATGAACAA TTGCCTCAGT 
CAGATGGAGC AGTTTCCAGT GAAAGTGCAT GACTTCCCCA GTGGAAATGG AACAGGGAGC 
AGTTTTTCTC TTAACAGAGG ATCCCAAGCT TTAAAATTCT TCAATACGCA TCAGTTAAAA 
TGCCAGCTGC AAAGACATCC AGACTGTGCT AATGTGAAAC AGTGGAAGGG TGGACCTGTG 
AAGATTGATC CTCTGGCTTT GGTTCAAGCC ATTGAAAGAT ATCTTGTTGT TAGAGGTTAT 
GGAAGAGTTA GAGAAGATGA TGAGGATAGT GATGATGATG GGTCAGATGA AGAAATAGAT 
GAATCCTTGG CTGCTCAATT CTTAAATTCA GGGAATGTGA GACATAGATT GCAGTTTTAC 
ATTGGGGATC ACTTGCTACC ATATAATATG ACTGTATATC AAGCAGTTAA GCTGTACAGT 
TTGCAAGCTG AGGAGGAGAG GGAATCGACT GATGATGAAA GCAATCCATT AGGAAGAGCT 
GGGATTTGGA CAAAAACACA TACCATTTGG TACAAACCTG TACGGGAGGA TGAAGATGGC 
AACAAAGACT GTGTTGGTGG TAAAAGAGGA AGAGCTCAAA CTGCTCCCAC AAAAACCTCA 
CCTAGGAATT CTAAAAAGCA TGATGAGTTG TGGCACGATG GTGTATGCCC TTCAGTATTG 
AATCCTTTAG AAGTTTACCT CATATCTACT CCTCCTGAAA ACATAACCTT TGAAGATCCT 
TCATTAGATG TTATTCTTCT TTTGAGAGTT TTACATGCTA TCAGTCGATA CTGGTATTAT 
TTGTATGATA ATGCAATATG CAAGGAGATT ATTCCAACTT CGGAATTTAT CAATAGTAAA 
CTGACAGCAA AAGCAAACAG ACAGCTTCAA GATCCACTGG TAATTATGAC AGGAAACATA 
CCGACTTGGC TGACAGAGCT TGGAAAAACA TGCCCATTTT TCTTTCCATT TGATACCCGG 
CAAATGCTGT TTTATGTAAC TGCTTTTGAT CGTGATCGAG CCATGCAAAG ACTACTGGAT 
ACTAATCCAG AAATCAATCA ATCAGATTCT CAGGATAGCA GAGTGGCACC ACGACTGGAC 
AGGAAAAAAC GCACTGTGAA CAGAGATGAG CTGTTGAAAC AGGCAGAATC TGTGATGCAG 
GATCTAGGCA GTTCAAGAGC CATGTTGGAA ATCCAGTATG AGAATGAAGT TGGCACAGGC 
CTGGGCCCTA CACTAGAGTT CTATGCACTT GTATCTCAGG AACTACAGAG AGCAGACTTG 
GGCCTTTGGA GGGGAGAAGA AGTAACTTTA GCCAATCCAA AAGGAAGCCA AGAAGGTACC 
AAGTATATTC ATAATCTCCA AGGCCTTTTT GCACTTCCTT TTGGTAGAAC AGCCAAGCCT 
GCTCACATTG CAAAAGTTAA AATGAAGTTC CGCTTTCTGG GAAAACTAAT GGCTAAGGCA 
ATCATGGATT TTAGACTGGT GGATCTTCCT CTTGGACTTC CTTTTTATAA ATGGATGTTA 
CGCCAGGAAA CTTCTTTGAC GTCACATGAC TTGTTCAGTA TTGATCCAGT AGTAGCCAAA 
TCAATATATC ACCTTGAAGA TATTGTAAGA CAAAAGAAAA GACTTGAGCA GGACAAAACA 
CAGACCAAAG AAAGTCTACA GTATGCATTG GAAACCCTGA CTATGAATGG CTGCTCAGTG 
GAGGATCTAG GGCTGGATTT CACACTTCCT GGATTTCCTA ACATCGAGCT GAAAAAAGGG 
GGAAAAGATA CACCAGTCAC CATCCACAAC TTGGAGGAGT ACCTCAGATT GGTTATATTC 
TGGGCACTTA ACGAAGGTGT TGCCAGACAG TTTGATTCTT TCAGAGATGG ATTTGAATCA 
GTCTTCCCCC TCAGTCATCT TCAATACTTT TATCCTGAGG AGTTGGAGCA ACTCTTATGT 
GGCAGCAAAA CAGACACATG GGATGCCAAG ACCTTAATGG AATGTTGCAG GCCAGATCAC 
GGTTATACAC ATGACAGTCG AGCAGTGAAG TATCTCTTTG AAATTCTCAG TAGTTTTGAT 
AGTGAGCAGC AGAGACTGTT TCTTCAGTTT GTGACCGGTA GTCCCAGGCT GCCAGTAGGA 
GGCTTTCGAA GTTTGAATCC TCCATTGACA ATTGTACGGA AGACATTTGA GTCTACAGAG 
AATCCAGATG ACTTCTTGCC CTCAGTAATG ACTTGTGTGA ACTATCTCAA ATTGCCGGAC 
TATTCAACTA TTGAGATAAT GCGTGAAAAA CTCTTGATAG CTGCAAGAGA AGGGCAGCAG 
TCATTCCATC TTTCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015132453.1
CDS	71..6268
Translation	MSNRPNNNPGGSLRRSQRNTAGAQPQDDAAGGRSHLGQAKHKAHSPPESRKSISKTPKVQSNTTSEQSKGHFSKRGCSSSAVLIPQEDSERVSTSEKQKTGQVPKKDNSRGVKRSASPDYSRTNSPSSAKKPKALQHAETSSESNKPHTKSKRRHLDQEQQKSTQLPSTSKAHTRKGGAAGSSRSQKRKRTESTSCIKSRSAVESTGAEEKSAKSSKLASKSVTSAKAGCSTITDSSSSASTSSSSSAVASASSTVPQGARVKQGKDQNKARRSRSASSPSPRRSSRDKEQSKTGGSSKFDWAARFSPKVSLPKTKLSLPGSSKSETSKPGPSGLQAKLASLRKSTKKRSESPPAELPSLRRSTRQKTTGSCASTSRRGSGLGKRGAAEARRQEKMADPDNNQDGVNSSAARTDETPQGAAASSSVAGAVGMTTSGESESDDSEMGRLQALLEARGLPPHLFGPLGPRMSQLFHRTIGSGASSKAQQLLQGLQATDESQQLQAVIEMCQLLVMGNEETLGGFPVKSVVPALITLLQMEHNFDIMNHACRALTYMMEALPRSSAVVVDAIPVFLEKLQVIQCIDVAEQALTALEMLSRRHSKAILQAGGLADCLLYLEFFSINAQRNALAIAANCCQSITPDEFHFVADSLPLLTQRLTHQDKKSVESTCLCFARLVDNFQHEENLLQQVASKDLLTNIQQLLVVTPPILSSGMFIMVVRMFSLMCSNCPTLAVQLMKQNIAETLHFLLCGASNGSCQEQIDLVPRSPQELYELTSLICELMPCLPKEGIFAVDTMLKKGNAQNTDGAIWQWRDDRGLWHPYNRIDSRIIEAAHQVGEDEISLSTLGRVYTIDFNSMQQINEDTGTARAIQRKPNPLANTNTSGHSELKKDDARAQLMKEDPELAKSFIKTLFGVLYEVYSSSAGPAVRHKCLRAILRIIYFADAELLKDVLKNHAVSSHIASMLSSQDLKIVVGALQMAEILMQKLPDIFSVYFRREGVMHQVKNLAESEALLTSPPKVCTNGSGTLGTTTTISTGTATAATNAAADLGSPSLQHSREDSLDLSPQGRLSDVLKRKRLPKRGPRRPKYSPPRDDDKVDNQAKSPTTTQSPKSSFLASLNPKTWGRLSTQSNSNNIEPARTAGVSGLARAASKDTISNNREKIKGWIKEQAHKFVDRYFSSENMDGSNPALNVLQRLCTATEQLNLQVDGGTECLVEIRSIVSESDVSSFEIQHSGFVKQLLLYLTSKSEKDAVSRDIRLKRFLHVFFSSPLPGEEPLGRLEPLENAPLLALVHKMNNCLSQMEQFPVKVHDFPSGNGTGSSFSLNRGSQALKFFNTHQLKCQLQRHPDCANVKQWKGGPVKIDPLALVQAIERYLVVRGYGRVREDDEDSDDDGSDEEIDESLAAQFLNSGNVRHRLQFYIGDHLLPYNMTVYQAVKLYSLQAEEERESTDDESNPLGRAGIWTKTHTIWYKPVREDEDGNKDCVGGKRGRAQTAPTKTSPRNSKKHDELWHDGVCPSVLNPLEVYLISTPPENITFEDPSLDVILLLRVLHAISRYWYYLYDNAICKEIIPTSEFINSKLTAKANRQLQDPLVIMTGNIPTWLTELGKTCPFFFPFDTRQMLFYVTAFDRDRAMQRLLDTNPEINQSDSQDSRVAPRLDRKKRTVNRDELLKQAESVMQDLGSSRAMLEIQYENEVGTGLGPTLEFYALVSQELQRADLGLWRGEEVTLANPKGSQEGTKYIHNLQGLFALPFGRTAKPAHIAKVKMKFRFLGKLMAKAIMDFRLVDLPLGLPFYKWMLRQETSLTSHDLFSIDPVVAKSIYHLEDIVRQKKRLEQDKTQTKESLQYALETLTMNGCSVEDLGLDFTLPGFPNIELKKGGKDTPVTIHNLEEYLRLVIFWALNEGVARQFDSFRDGFESVFPLSHLQYFYPEELEQLLCGSKTDTWDAKTLMECCRPDHGYTHDSRAVKYLFEILSSFDSEQQRLFLQFVTGSPRLPVGGFRSLNPPLTIVRKTFESTENPDDFLPSVMTCVNYLKLPDYSTIEIMREKLLIAAREGQQSFHLS

Target ORF information:

RefSeq Version	XM_015276967.2
Organism	Gallus gallus(chicken)
Definition	Gallus gallus thyroid hormone receptor interactor 12 (TRIP12), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015276967.2

ORF Insert Sequence:

ATGTCCAACC GGCCTAATAA CAATCCAGGG GGGTCACTGC GACGTTCACA GAGGAACACT 
GCTGGGGCCC AGCCACAAGA CGACGCAGCA GGAGGAAGGT CACATTTAGG GCAGGCAAAA 
CATAAGGCAC ATAGCCCTCC TGAGAGTAGA AAATCTATTT CAAAGACACC CAAAGTGCAG 
TCTAATACTA CTTCTGAGCA GTCCAAGGGA CACTTTTCTA AAAGAGGCTG TAGTTCATCT 
GCTGTTTTAA TACCACAAGA AGATTCAGAA AGAGTCAGTA CTTCAGAAAA GCAAAAAACG 
GGGCAAGTGC CTAAGAAAGA CAATTCTCGA GGAGTTAAAC GCAGTGCTAG TCCAGATTAC 
AGCAGGACCA ATTCTCCTAG CTCTGCTAAA AAACCTAAAG CACTTCAACA CGCCGAAACT 
TCCTCGGAAA GTAACAAGCC ACATACTAAA TCCAAGAGAA GACACTTAGA CCAAGAACAG 
CAGAAGTCTA CACAATTGCC ATCAACAAGC AAGGCTCACA CCAGAAAGGG TGGAGCTGCT 
GGTAGCTCCC GAAGTCAGAA AAGGAAAAGG ACAGAGAGTA CGTCTTGTAT AAAGAGTCGT 
TCAGCAGTTG AATCAACTGG AGCTGAAGAG AAGTCAGCAA AATCCTCCAA GCTGGCTTCA 
AAATCGGTGA CCTCAGCCAA AGCTGGGTGT AGCACCATCA CTGATTCTTC TTCTTCAGCT 
TCTACATCCT CTTCCTCTTC TGCTGTTGCC TCTGCTTCTT CCACTGTTCC TCAGGGTGCC 
AGAGTGAAAC AGGGTAAGGA CCAGAATAAG GCTAGACGTT CACGTTCTGC GTCCAGCCCT 
AGTCCAAGAA GGAGCAGCAG GGACAAAGAA CAGAGCAAAA CAGGTGGCTC TTCAAAGTTT 
GACTGGGCTG CTCGATTCAG TCCAAAAGTT AGTCTCCCTA AAACAAAACT GTCTCTACCA 
GGCTCTTCTA AGTCAGAGAC CTCAAAACCT GGACCTTCAG GACTACAGGC TAAGCTAGCA 
AGCCTAAGAA AATCCACAAA GAAGCGCAGT GAATCACCAC CTGCTGAGCT CCCCAGTTTA 
CGGCGGAGCA CACGGCAAAA GACCACGGGC TCCTGTGCGA GTACCAGTCG GCGGGGCTCT 
GGCCTGGGCA AAAGAGGAGC AGCTGAAGCT CGCCGACAGG AGAAGATGGC TGATCCTGAC 
AACAACCAGG ATGGAGTTAA CTCTTCAGCT GCACGTACAG ATGAGACTCC CCAGGGAGCT 
GCAGCTTCTA GCTCTGTTGC TGGGGCTGTA GGTATGACAA CATCTGGAGA AAGTGAGTCA 
GATGACTCTG AGATGGGAAG ACTACAAGCT CTGTTAGAGG CTAGGGGTCT TCCTCCTCAC 
CTGTTTGGCC CTCTTGGTCC TCGGATGTCG CAGCTCTTCC ACAGGACAAT TGGAAGTGGA 
GCTAGTTCTA AAGCCCAGCA GCTTTTACAA GGTCTCCAAG CCACTGATGA AAGTCAGCAA 
CTACAGGCAG TTATTGAGAT GTGCCAGCTG TTGGTCATGG GAAATGAAGA AACACTGGGA 
GGATTTCCAG TCAAGAGTGT TGTGCCAGCT TTGATAACAT TGCTGCAGAT GGAGCATAAC 
TTTGACATCA TGAACCATGC TTGTCGAGCC TTAACATACA TGATGGAAGC ACTTCCCAGA 
TCATCTGCTG TAGTGGTAGA TGCAATTCCT GTCTTCTTGG AAAAGCTGCA AGTTATTCAG 
TGCATTGATG TGGCAGAGCA GGCACTGACA GCCCTAGAGA TGCTATCACG CAGGCATAGT 
AAAGCCATTC TGCAGGCAGG TGGGTTGGCA GACTGCTTGC TGTATCTGGA ATTCTTCAGT 
ATAAATGCGC AGAGGAATGC ACTAGCTATT GCTGCCAATT GCTGCCAGAG TATAACACCT 
GATGAGTTTC ACTTTGTGGC AGACTCTTTG CCACTGCTTA CACAAAGGTT AACCCATCAG 
GACAAGAAGT CTGTTGAAAG CACTTGTCTC TGTTTTGCAC GGCTGGTAGA CAATTTCCAG 
CATGAGGAGA ACTTGCTGCA GCAGGTTGCT TCCAAGGACT TGTTAACAAA CATCCAGCAA 
CTCTTGGTAG TGACACCTCC TATCCTGAGC TCAGGAATGT TCATCATGGT GGTGCGCATG 
TTTTCCTTAA TGTGTTCCAA TTGTCCAACG CTTGCAGTCC AACTTATGAA GCAAAACATT 
GCAGAAACAC TTCACTTTCT CCTTTGTGGA GCCTCAAATG GGAGTTGTCA AGAACAAATT 
GACCTTGTTC CACGAAGTCC TCAAGAACTC TATGAGCTTA CTTCTCTTAT CTGTGAACTG 
ATGCCTTGTC TGCCAAAAGA GGGGATATTT GCTGTTGATA CTATGCTAAA GAAAGGAAAT 
GCACAAAATA CAGATGGTGC GATATGGCAA TGGCGAGATG ACAGGGGTCT CTGGCATCCC 
TATAACAGGA TTGATAGTCG AATAATAGAG GCAGCTCATC AGGTTGGTGA GGATGAGATA 
AGCCTGTCGA CACTTGGACG TGTCTATACT ATTGATTTTA ATTCTATGCA GCAAATAAAT 
GAGGATACTG GAACAGCACG TGCCATTCAG CGGAAACCAA ACCCTTTAGC CAATACAAAC 
ACTAGTGGAC ATTCAGAATT GAAGAAGGAT GATGCTCGAG CACAGCTAAT GAAAGAAGAC 
CCAGAATTGG CAAAATCCTT TATCAAAACA CTGTTTGGTG TTCTTTATGA AGTGTATAGT 
TCTTCAGCTG GACCTGCTGT TAGACACAAG TGCCTTAGAG CAATACTTAG AATAATTTAT 
TTTGCTGATG CTGAACTCCT GAAGGATGTG CTGAAAAACC ATGCTGTGTC AAGTCACATT 
GCCTCCATGC TGTCAAGTCA AGACCTTAAG ATAGTAGTTG GAGCCCTGCA GATGGCAGAA 
ATCTTAATGC AGAAGCTACC TGACATTTTT AGTGTTTACT TCAGAAGAGA AGGAGTGATG 
CATCAAGTGA AAAACTTAGC GGAGTCTGAG GCTTTGCTAA CGAGCCCACC AAAAGTATGC 
ACTAATGGAT CAGGAACACT GGGTACCACT ACAACTATAA GTACTGGAAC AGCCACTGCT 
GCCACTAATG CAGCTGCAGA TTTGGGCTCT CCCAGCTTAC AACACAGCCG GGAGGATTCT 
TTAGATCTGA GCCCACAGGG ACGACTGAGT GATGTTCTGA AGAGAAAAAG ACTGCCAAAA 
CGAGGGCCAA GGAGACCAAA ATATTCTCCC CCAAGAGATG ATGACAAAGT AGACAATCAA 
GCAAAAAGCC CCACGACTAC TCAGTCTCCT AAATCTTCTT TCTTAGCAAG TTTAAATCCC 
AAAACATGGG GAAGATTAAG TACACAGTCT AACAGTAATA ACATTGAACC AGCAAGAACA 
GCAGGTGTAA GTGGTCTTGC AAGAGCTGCT TCTAAGGATA CAATTTCCAA TAACAGAGAA 
AAAATTAAGG GTTGGATTAA GGAGCAAGCC CATAAATTTG TAGACCGTTA TTTTAGTTCG 
GAAAACATGG ATGGAAGCAA TCCTGCACTA AATGTATTAC AGAGACTTTG CACTGCAACT 
GAACAACTTA ACCTCCAGGT GGATGGTGGA ACAGAGTGCC TTGTAGAAAT CCGTAGCATT 
GTCTCGGAGT CTGACGTCTC CTCATTTGAA ATCCAGCATA GTGGATTTGT GAAACAACTG 
CTGCTTTATT TGACATCTAA AAGTGAGAAA GATGCTGTAA GCAGGGATAT CAGATTGAAA 
AGATTTCTTC ATGTATTTTT CTCCTCTCCA CTTCCTGGAG AAGAACCCCT TGGAAGATTA 
GAGCCATTAG AAAATGCACC TTTGTTGGCA TTAGTCCATA AAATGAACAA TTGCCTCAGT 
CAGATGGAGC AGTTTCCAGT GAAAGTGCAT GACTTCCCCA GTGGAAATGG AACAGGGAGC 
AGTTTTTCTC TTAACAGAGG ATCCCAAGCT TTAAAATTCT TCAATACGCA TCAGTTAAAA 
TGCCAGCTGC AAAGACATCC AGACTGTGCT AATGTGAAAC AGTGGAAGGG TGGACCTGTG 
AAGATTGATC CTCTGGCTTT GGTTCAAGCC ATTGAAAGAT ATCTTGTTGT TAGAGGTTAT 
GGAAGAGTTA GAGAAGATGA TGAGGATAGT GATGATGATG GGTCAGATGA AGAAATAGAT 
GAATCCTTGG CTGCTCAATT CTTAAATTCA GGGAATGTGA GACATAGATT GCAGTTTTAC 
ATTGGGGATC ACTTGCTACC ATATAATATG ACTGTATATC AAGCAGTTAA GCTGTACAGT 
TTGCAAGCTG AGGAGGAGAG GGAATCGACT GATGATGAAA GCAATCCATT AGGAAGAGCT 
GGGATTTGGA CAAAAACACA TACCATTTGG TACAAACCTG TACGGGAGGA TGAAGATGGC 
AACAAAGACT GTGTTGGTGG TAAAAGAGGA AGAGCTCAAA CTGCTCCCAC AAAAACCTCA 
CCTAGGAATT CTAAAAAGCA TGATGAGTTG TGGCACGATG GTGTATGCCC TTCAGTATTG 
AATCCTTTAG AAGTTTACCT CATATCTACT CCTCCTGAAA ACATAACCTT TGAAGATCCT 
TCATTAGATG TTATTCTTCT TTTGAGAGTT TTACATGCTA TCAGTCGATA CTGGTATTAT 
TTGTATGATA ATGCAATATG CAAGGAGATT ATTCCAACTT CGGAATTTAT CAATAGTAAA 
CTGACAGCAA AAGCAAACAG ACAGCTTCAA GATCCACTGG TAATTATGAC AGGAAACATA 
CCGACTTGGC TGACAGAGCT TGGAAAAACA TGCCCATTTT TCTTTCCATT TGATACCCGG 
CAAATGCTGT TTTATGTAAC TGCTTTTGAT CGTGATCGAG CCATGCAAAG ACTACTGGAT 
ACTAATCCAG AAATCAATCA ATCAGATTCT CAGGATAGCA GAGTGGCACC ACGACTGGAC 
AGGAAAAAAC GCACTGTGAA CAGAGATGAG CTGTTGAAAC AGGCAGAATC TGTGATGCAG 
GATCTAGGCA GTTCAAGAGC CATGTTGGAA ATCCAGTATG AGAATGAAGT TGGCACAGGC 
CTGGGCCCTA CACTAGAGTT CTATGCACTT GTATCTCAGG AACTACAGAG AGCAGACTTG 
GGCCTTTGGA GGGGAGAAGA AGTAACTTTA GCCAATCCAA AAGGAAGCCA AGAAGGTACC 
AAGTATATTC ATAATCTCCA AGGCCTTTTT GCACTTCCTT TTGGTAGAAC AGCCAAGCCT 
GCTCACATTG CAAAAGTTAA AATGAAGTTC CGCTTTCTGG GAAAACTAAT GGCTAAGGCA 
ATCATGGATT TTAGACTGGT GGATCTTCCT CTTGGACTTC CTTTTTATAA ATGGATGTTA 
CGCCAGGAAA CTTCTTTGAC GTCACATGAC TTGTTCAGTA TTGATCCAGT AGTAGCCAAA 
TCAATATATC ACCTTGAAGA TATTGTAAGA CAAAAGAAAA GACTTGAGCA GGACAAAACA 
CAGACCAAAG AAAGTCTACA GTATGCATTG GAAACCCTGA CTATGAATGG CTGCTCAGTG 
GAGGATCTAG GGCTGGATTT CACACTTCCT GGATTTCCTA ACATCGAGCT GAAAAAAGGG 
GGAAAAGATA CACCAGTCAC CATCCACAAC TTGGAGGAGT ACCTCAGATT GGTTATATTC 
TGGGCACTTA ACGAAGGTGT TGCCAGACAG TTTGATTCTT TCAGAGATGG ATTTGAATCA 
GTCTTCCCCC TCAGTCATCT TCAATACTTT TATCCTGAGG AGTTGGAGCA ACTCTTATGT 
GGCAGCAAAA CAGACACATG GGATGCCAAG ACCTTAATGG AATGTTGCAG GCCAGATCAC 
GGTTATACAC ATGACAGTCG AGCAGTGAAG TATCTCTTTG AAATTCTCAG TAGTTTTGAT 
AGTGAGCAGC AGAGACTGTT TCTTCAGTTT GTGACCGGTA GTCCCAGGCT GCCAGTAGGA 
GGCTTTCGAA GTTTGAATCC TCCATTGACA ATTGTACGGA AGACATTTGA GTCTACAGAG 
AATCCAGATG ACTTCTTGCC CTCAGTAATG ACTTGTGTGA ACTATCTCAA ATTGCCGGAC 
TATTCAACTA TTGAGATAAT GCGTGAAAAA CTCTTGATAG CTGCAAGAGA AGGGCAGCAG 
TCATTCCATC TTTCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TRIP12 ( XM_015276965.1 ) cDNA ORF clone, Gallus gallus(chicken) -> XP_015132451.1 Gallus gallus thyroid hormone receptor interactor 12 (TRIP12), transcript variant X3, mRNA.

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CloneID	OGa12502
Clone ID Related Accession (Same CDS sequence)	XM_015276964.1 , XM_015276968.2 , XM_015276967.1 , XM_015276968.1 , XM_004937064.3 , XM_015276964.2 , XM_015276966.2 , XM_004937064.2 , XM_015276966.1 , XM_015276967.2 , XM_015276965.1
Accession Version	XM_015276965.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6198bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-03
Organism	Gallus gallus(chicken)
Product	E3 ubiquitin-protein ligase TRIP12 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_001471743.3) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Gallus gallus Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCCAACC GGCCTAATAA CAATCCAGGG GGGTCACTGC GACGTTCACA GAGGAACACT 
GCTGGGGCCC AGCCACAAGA CGACGCAGCA GGAGGAAGGT CACATTTAGG GCAGGCAAAA 
CATAAGGCAC ATAGCCCTCC TGAGAGTAGA AAATCTATTT CAAAGACACC CAAAGTGCAG 
TCTAATACTA CTTCTGAGCA GTCCAAGGGA CACTTTTCTA AAAGAGGCTG TAGTTCATCT 
GCTGTTTTAA TACCACAAGA AGATTCAGAA AGAGTCAGTA CTTCAGAAAA GCAAAAAACG 
GGGCAAGTGC CTAAGAAAGA CAATTCTCGA GGAGTTAAAC GCAGTGCTAG TCCAGATTAC 
AGCAGGACCA ATTCTCCTAG CTCTGCTAAA AAACCTAAAG CACTTCAACA CGCCGAAACT 
TCCTCGGAAA GTAACAAGCC ACATACTAAA TCCAAGAGAA GACACTTAGA CCAAGAACAG 
CAGAAGTCTA CACAATTGCC ATCAACAAGC AAGGCTCACA CCAGAAAGGG TGGAGCTGCT 
GGTAGCTCCC GAAGTCAGAA AAGGAAAAGG ACAGAGAGTA CGTCTTGTAT AAAGAGTCGT 
TCAGCAGTTG AATCAACTGG AGCTGAAGAG AAGTCAGCAA AATCCTCCAA GCTGGCTTCA 
AAATCGGTGA CCTCAGCCAA AGCTGGGTGT AGCACCATCA CTGATTCTTC TTCTTCAGCT 
TCTACATCCT CTTCCTCTTC TGCTGTTGCC TCTGCTTCTT CCACTGTTCC TCAGGGTGCC 
AGAGTGAAAC AGGGTAAGGA CCAGAATAAG GCTAGACGTT CACGTTCTGC GTCCAGCCCT 
AGTCCAAGAA GGAGCAGCAG GGACAAAGAA CAGAGCAAAA CAGGTGGCTC TTCAAAGTTT 
GACTGGGCTG CTCGATTCAG TCCAAAAGTT AGTCTCCCTA AAACAAAACT GTCTCTACCA 
GGCTCTTCTA AGTCAGAGAC CTCAAAACCT GGACCTTCAG GACTACAGGC TAAGCTAGCA 
AGCCTAAGAA AATCCACAAA GAAGCGCAGT GAATCACCAC CTGCTGAGCT CCCCAGTTTA 
CGGCGGAGCA CACGGCAAAA GACCACGGGC TCCTGTGCGA GTACCAGTCG GCGGGGCTCT 
GGCCTGGGCA AAAGAGGAGC AGCTGAAGCT CGCCGACAGG AGAAGATGGC TGATCCTGAC 
AACAACCAGG ATGGAGTTAA CTCTTCAGCT GCACGTACAG ATGAGACTCC CCAGGGAGCT 
GCAGCTTCTA GCTCTGTTGC TGGGGCTGTA GGTATGACAA CATCTGGAGA AAGTGAGTCA 
GATGACTCTG AGATGGGAAG ACTACAAGCT CTGTTAGAGG CTAGGGGTCT TCCTCCTCAC 
CTGTTTGGCC CTCTTGGTCC TCGGATGTCG CAGCTCTTCC ACAGGACAAT TGGAAGTGGA 
GCTAGTTCTA AAGCCCAGCA GCTTTTACAA GGTCTCCAAG CCACTGATGA AAGTCAGCAA 
CTACAGGCAG TTATTGAGAT GTGCCAGCTG TTGGTCATGG GAAATGAAGA AACACTGGGA 
GGATTTCCAG TCAAGAGTGT TGTGCCAGCT TTGATAACAT TGCTGCAGAT GGAGCATAAC 
TTTGACATCA TGAACCATGC TTGTCGAGCC TTAACATACA TGATGGAAGC ACTTCCCAGA 
TCATCTGCTG TAGTGGTAGA TGCAATTCCT GTCTTCTTGG AAAAGCTGCA AGTTATTCAG 
TGCATTGATG TGGCAGAGCA GGCACTGACA GCCCTAGAGA TGCTATCACG CAGGCATAGT 
AAAGCCATTC TGCAGGCAGG TGGGTTGGCA GACTGCTTGC TGTATCTGGA ATTCTTCAGT 
ATAAATGCGC AGAGGAATGC ACTAGCTATT GCTGCCAATT GCTGCCAGAG TATAACACCT 
GATGAGTTTC ACTTTGTGGC AGACTCTTTG CCACTGCTTA CACAAAGGTT AACCCATCAG 
GACAAGAAGT CTGTTGAAAG CACTTGTCTC TGTTTTGCAC GGCTGGTAGA CAATTTCCAG 
CATGAGGAGA ACTTGCTGCA GCAGGTTGCT TCCAAGGACT TGTTAACAAA CATCCAGCAA 
CTCTTGGTAG TGACACCTCC TATCCTGAGC TCAGGAATGT TCATCATGGT GGTGCGCATG 
TTTTCCTTAA TGTGTTCCAA TTGTCCAACG CTTGCAGTCC AACTTATGAA GCAAAACATT 
GCAGAAACAC TTCACTTTCT CCTTTGTGGA GCCTCAAATG GGAGTTGTCA AGAACAAATT 
GACCTTGTTC CACGAAGTCC TCAAGAACTC TATGAGCTTA CTTCTCTTAT CTGTGAACTG 
ATGCCTTGTC TGCCAAAAGA GGGGATATTT GCTGTTGATA CTATGCTAAA GAAAGGAAAT 
GCACAAAATA CAGATGGTGC GATATGGCAA TGGCGAGATG ACAGGGGTCT CTGGCATCCC 
TATAACAGGA TTGATAGTCG AATAATAGAG GCAGCTCATC AGGTTGGTGA GGATGAGATA 
AGCCTGTCGA CACTTGGACG TGTCTATACT ATTGATTTTA ATTCTATGCA GCAAATAAAT 
GAGGATACTG GAACAGCACG TGCCATTCAG CGGAAACCAA ACCCTTTAGC CAATACAAAC 
ACTAGTGGAC ATTCAGAATT GAAGAAGGAT GATGCTCGAG CACAGCTAAT GAAAGAAGAC 
CCAGAATTGG CAAAATCCTT TATCAAAACA CTGTTTGGTG TTCTTTATGA AGTGTATAGT 
TCTTCAGCTG GACCTGCTGT TAGACACAAG TGCCTTAGAG CAATACTTAG AATAATTTAT 
TTTGCTGATG CTGAACTCCT GAAGGATGTG CTGAAAAACC ATGCTGTGTC AAGTCACATT 
GCCTCCATGC TGTCAAGTCA AGACCTTAAG ATAGTAGTTG GAGCCCTGCA GATGGCAGAA 
ATCTTAATGC AGAAGCTACC TGACATTTTT AGTGTTTACT TCAGAAGAGA AGGAGTGATG 
CATCAAGTGA AAAACTTAGC GGAGTCTGAG GCTTTGCTAA CGAGCCCACC AAAAGTATGC 
ACTAATGGAT CAGGAACACT GGGTACCACT ACAACTATAA GTACTGGAAC AGCCACTGCT 
GCCACTAATG CAGCTGCAGA TTTGGGCTCT CCCAGCTTAC AACACAGCCG GGAGGATTCT 
TTAGATCTGA GCCCACAGGG ACGACTGAGT GATGTTCTGA AGAGAAAAAG ACTGCCAAAA 
CGAGGGCCAA GGAGACCAAA ATATTCTCCC CCAAGAGATG ATGACAAAGT AGACAATCAA 
GCAAAAAGCC CCACGACTAC TCAGTCTCCT AAATCTTCTT TCTTAGCAAG TTTAAATCCC 
AAAACATGGG GAAGATTAAG TACACAGTCT AACAGTAATA ACATTGAACC AGCAAGAACA 
GCAGGTGTAA GTGGTCTTGC AAGAGCTGCT TCTAAGGATA CAATTTCCAA TAACAGAGAA 
AAAATTAAGG GTTGGATTAA GGAGCAAGCC CATAAATTTG TAGACCGTTA TTTTAGTTCG 
GAAAACATGG ATGGAAGCAA TCCTGCACTA AATGTATTAC AGAGACTTTG CACTGCAACT 
GAACAACTTA ACCTCCAGGT GGATGGTGGA ACAGAGTGCC TTGTAGAAAT CCGTAGCATT 
GTCTCGGAGT CTGACGTCTC CTCATTTGAA ATCCAGCATA GTGGATTTGT GAAACAACTG 
CTGCTTTATT TGACATCTAA AAGTGAGAAA GATGCTGTAA GCAGGGATAT CAGATTGAAA 
AGATTTCTTC ATGTATTTTT CTCCTCTCCA CTTCCTGGAG AAGAACCCCT TGGAAGATTA 
GAGCCATTAG AAAATGCACC TTTGTTGGCA TTAGTCCATA AAATGAACAA TTGCCTCAGT 
CAGATGGAGC AGTTTCCAGT GAAAGTGCAT GACTTCCCCA GTGGAAATGG AACAGGGAGC 
AGTTTTTCTC TTAACAGAGG ATCCCAAGCT TTAAAATTCT TCAATACGCA TCAGTTAAAA 
TGCCAGCTGC AAAGACATCC AGACTGTGCT AATGTGAAAC AGTGGAAGGG TGGACCTGTG 
AAGATTGATC CTCTGGCTTT GGTTCAAGCC ATTGAAAGAT ATCTTGTTGT TAGAGGTTAT 
GGAAGAGTTA GAGAAGATGA TGAGGATAGT GATGATGATG GGTCAGATGA AGAAATAGAT 
GAATCCTTGG CTGCTCAATT CTTAAATTCA GGGAATGTGA GACATAGATT GCAGTTTTAC 
ATTGGGGATC ACTTGCTACC ATATAATATG ACTGTATATC AAGCAGTTAA GCTGTACAGT 
TTGCAAGCTG AGGAGGAGAG GGAATCGACT GATGATGAAA GCAATCCATT AGGAAGAGCT 
GGGATTTGGA CAAAAACACA TACCATTTGG TACAAACCTG TACGGGAGGA TGAAGATGGC 
AACAAAGACT GTGTTGGTGG TAAAAGAGGA AGAGCTCAAA CTGCTCCCAC AAAAACCTCA 
CCTAGGAATT CTAAAAAGCA TGATGAGTTG TGGCACGATG GTGTATGCCC TTCAGTATTG 
AATCCTTTAG AAGTTTACCT CATATCTACT CCTCCTGAAA ACATAACCTT TGAAGATCCT 
TCATTAGATG TTATTCTTCT TTTGAGAGTT TTACATGCTA TCAGTCGATA CTGGTATTAT 
TTGTATGATA ATGCAATATG CAAGGAGATT ATTCCAACTT CGGAATTTAT CAATAGTAAA 
CTGACAGCAA AAGCAAACAG ACAGCTTCAA GATCCACTGG TAATTATGAC AGGAAACATA 
CCGACTTGGC TGACAGAGCT TGGAAAAACA TGCCCATTTT TCTTTCCATT TGATACCCGG 
CAAATGCTGT TTTATGTAAC TGCTTTTGAT CGTGATCGAG CCATGCAAAG ACTACTGGAT 
ACTAATCCAG AAATCAATCA ATCAGATTCT CAGGATAGCA GAGTGGCACC ACGACTGGAC 
AGGAAAAAAC GCACTGTGAA CAGAGATGAG CTGTTGAAAC AGGCAGAATC TGTGATGCAG 
GATCTAGGCA GTTCAAGAGC CATGTTGGAA ATCCAGTATG AGAATGAAGT TGGCACAGGC 
CTGGGCCCTA CACTAGAGTT CTATGCACTT GTATCTCAGG AACTACAGAG AGCAGACTTG 
GGCCTTTGGA GGGGAGAAGA AGTAACTTTA GCCAATCCAA AAGGAAGCCA AGAAGGTACC 
AAGTATATTC ATAATCTCCA AGGCCTTTTT GCACTTCCTT TTGGTAGAAC AGCCAAGCCT 
GCTCACATTG CAAAAGTTAA AATGAAGTTC CGCTTTCTGG GAAAACTAAT GGCTAAGGCA 
ATCATGGATT TTAGACTGGT GGATCTTCCT CTTGGACTTC CTTTTTATAA ATGGATGTTA 
CGCCAGGAAA CTTCTTTGAC GTCACATGAC TTGTTCAGTA TTGATCCAGT AGTAGCCAAA 
TCAATATATC ACCTTGAAGA TATTGTAAGA CAAAAGAAAA GACTTGAGCA GGACAAAACA 
CAGACCAAAG AAAGTCTACA GTATGCATTG GAAACCCTGA CTATGAATGG CTGCTCAGTG 
GAGGATCTAG GGCTGGATTT CACACTTCCT GGATTTCCTA ACATCGAGCT GAAAAAAGGG 
GGAAAAGATA CACCAGTCAC CATCCACAAC TTGGAGGAGT ACCTCAGATT GGTTATATTC 
TGGGCACTTA ACGAAGGTGT TGCCAGACAG TTTGATTCTT TCAGAGATGG ATTTGAATCA 
GTCTTCCCCC TCAGTCATCT TCAATACTTT TATCCTGAGG AGTTGGAGCA ACTCTTATGT 
GGCAGCAAAA CAGACACATG GGATGCCAAG ACCTTAATGG AATGTTGCAG GCCAGATCAC 
GGTTATACAC ATGACAGTCG AGCAGTGAAG TATCTCTTTG AAATTCTCAG TAGTTTTGAT 
AGTGAGCAGC AGAGACTGTT TCTTCAGTTT GTGACCGGTA GTCCCAGGCT GCCAGTAGGA 
GGCTTTCGAA GTTTGAATCC TCCATTGACA ATTGTACGGA AGACATTTGA GTCTACAGAG 
AATCCAGATG ACTTCTTGCC CTCAGTAATG ACTTGTGTGA ACTATCTCAA ATTGCCGGAC 
TATTCAACTA TTGAGATAAT GCGTGAAAAA CTCTTGATAG CTGCAAGAGA AGGGCAGCAG 
TCATTCCATC TTTCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015132451.1
CDS	175..6372
Translation	MSNRPNNNPGGSLRRSQRNTAGAQPQDDAAGGRSHLGQAKHKAHSPPESRKSISKTPKVQSNTTSEQSKGHFSKRGCSSSAVLIPQEDSERVSTSEKQKTGQVPKKDNSRGVKRSASPDYSRTNSPSSAKKPKALQHAETSSESNKPHTKSKRRHLDQEQQKSTQLPSTSKAHTRKGGAAGSSRSQKRKRTESTSCIKSRSAVESTGAEEKSAKSSKLASKSVTSAKAGCSTITDSSSSASTSSSSSAVASASSTVPQGARVKQGKDQNKARRSRSASSPSPRRSSRDKEQSKTGGSSKFDWAARFSPKVSLPKTKLSLPGSSKSETSKPGPSGLQAKLASLRKSTKKRSESPPAELPSLRRSTRQKTTGSCASTSRRGSGLGKRGAAEARRQEKMADPDNNQDGVNSSAARTDETPQGAAASSSVAGAVGMTTSGESESDDSEMGRLQALLEARGLPPHLFGPLGPRMSQLFHRTIGSGASSKAQQLLQGLQATDESQQLQAVIEMCQLLVMGNEETLGGFPVKSVVPALITLLQMEHNFDIMNHACRALTYMMEALPRSSAVVVDAIPVFLEKLQVIQCIDVAEQALTALEMLSRRHSKAILQAGGLADCLLYLEFFSINAQRNALAIAANCCQSITPDEFHFVADSLPLLTQRLTHQDKKSVESTCLCFARLVDNFQHEENLLQQVASKDLLTNIQQLLVVTPPILSSGMFIMVVRMFSLMCSNCPTLAVQLMKQNIAETLHFLLCGASNGSCQEQIDLVPRSPQELYELTSLICELMPCLPKEGIFAVDTMLKKGNAQNTDGAIWQWRDDRGLWHPYNRIDSRIIEAAHQVGEDEISLSTLGRVYTIDFNSMQQINEDTGTARAIQRKPNPLANTNTSGHSELKKDDARAQLMKEDPELAKSFIKTLFGVLYEVYSSSAGPAVRHKCLRAILRIIYFADAELLKDVLKNHAVSSHIASMLSSQDLKIVVGALQMAEILMQKLPDIFSVYFRREGVMHQVKNLAESEALLTSPPKVCTNGSGTLGTTTTISTGTATAATNAAADLGSPSLQHSREDSLDLSPQGRLSDVLKRKRLPKRGPRRPKYSPPRDDDKVDNQAKSPTTTQSPKSSFLASLNPKTWGRLSTQSNSNNIEPARTAGVSGLARAASKDTISNNREKIKGWIKEQAHKFVDRYFSSENMDGSNPALNVLQRLCTATEQLNLQVDGGTECLVEIRSIVSESDVSSFEIQHSGFVKQLLLYLTSKSEKDAVSRDIRLKRFLHVFFSSPLPGEEPLGRLEPLENAPLLALVHKMNNCLSQMEQFPVKVHDFPSGNGTGSSFSLNRGSQALKFFNTHQLKCQLQRHPDCANVKQWKGGPVKIDPLALVQAIERYLVVRGYGRVREDDEDSDDDGSDEEIDESLAAQFLNSGNVRHRLQFYIGDHLLPYNMTVYQAVKLYSLQAEEERESTDDESNPLGRAGIWTKTHTIWYKPVREDEDGNKDCVGGKRGRAQTAPTKTSPRNSKKHDELWHDGVCPSVLNPLEVYLISTPPENITFEDPSLDVILLLRVLHAISRYWYYLYDNAICKEIIPTSEFINSKLTAKANRQLQDPLVIMTGNIPTWLTELGKTCPFFFPFDTRQMLFYVTAFDRDRAMQRLLDTNPEINQSDSQDSRVAPRLDRKKRTVNRDELLKQAESVMQDLGSSRAMLEIQYENEVGTGLGPTLEFYALVSQELQRADLGLWRGEEVTLANPKGSQEGTKYIHNLQGLFALPFGRTAKPAHIAKVKMKFRFLGKLMAKAIMDFRLVDLPLGLPFYKWMLRQETSLTSHDLFSIDPVVAKSIYHLEDIVRQKKRLEQDKTQTKESLQYALETLTMNGCSVEDLGLDFTLPGFPNIELKKGGKDTPVTIHNLEEYLRLVIFWALNEGVARQFDSFRDGFESVFPLSHLQYFYPEELEQLLCGSKTDTWDAKTLMECCRPDHGYTHDSRAVKYLFEILSSFDSEQQRLFLQFVTGSPRLPVGGFRSLNPPLTIVRKTFESTENPDDFLPSVMTCVNYLKLPDYSTIEIMREKLLIAAREGQQSFHLS

Target ORF information:

RefSeq Version	XM_015276965.1
Organism	Gallus gallus(chicken)
Definition	Gallus gallus thyroid hormone receptor interactor 12 (TRIP12), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015276965.1

ORF Insert Sequence:

ATGTCCAACC GGCCTAATAA CAATCCAGGG GGGTCACTGC GACGTTCACA GAGGAACACT 
GCTGGGGCCC AGCCACAAGA CGACGCAGCA GGAGGAAGGT CACATTTAGG GCAGGCAAAA 
CATAAGGCAC ATAGCCCTCC TGAGAGTAGA AAATCTATTT CAAAGACACC CAAAGTGCAG 
TCTAATACTA CTTCTGAGCA GTCCAAGGGA CACTTTTCTA AAAGAGGCTG TAGTTCATCT 
GCTGTTTTAA TACCACAAGA AGATTCAGAA AGAGTCAGTA CTTCAGAAAA GCAAAAAACG 
GGGCAAGTGC CTAAGAAAGA CAATTCTCGA GGAGTTAAAC GCAGTGCTAG TCCAGATTAC 
AGCAGGACCA ATTCTCCTAG CTCTGCTAAA AAACCTAAAG CACTTCAACA CGCCGAAACT 
TCCTCGGAAA GTAACAAGCC ACATACTAAA TCCAAGAGAA GACACTTAGA CCAAGAACAG 
CAGAAGTCTA CACAATTGCC ATCAACAAGC AAGGCTCACA CCAGAAAGGG TGGAGCTGCT 
GGTAGCTCCC GAAGTCAGAA AAGGAAAAGG ACAGAGAGTA CGTCTTGTAT AAAGAGTCGT 
TCAGCAGTTG AATCAACTGG AGCTGAAGAG AAGTCAGCAA AATCCTCCAA GCTGGCTTCA 
AAATCGGTGA CCTCAGCCAA AGCTGGGTGT AGCACCATCA CTGATTCTTC TTCTTCAGCT 
TCTACATCCT CTTCCTCTTC TGCTGTTGCC TCTGCTTCTT CCACTGTTCC TCAGGGTGCC 
AGAGTGAAAC AGGGTAAGGA CCAGAATAAG GCTAGACGTT CACGTTCTGC GTCCAGCCCT 
AGTCCAAGAA GGAGCAGCAG GGACAAAGAA CAGAGCAAAA CAGGTGGCTC TTCAAAGTTT 
GACTGGGCTG CTCGATTCAG TCCAAAAGTT AGTCTCCCTA AAACAAAACT GTCTCTACCA 
GGCTCTTCTA AGTCAGAGAC CTCAAAACCT GGACCTTCAG GACTACAGGC TAAGCTAGCA 
AGCCTAAGAA AATCCACAAA GAAGCGCAGT GAATCACCAC CTGCTGAGCT CCCCAGTTTA 
CGGCGGAGCA CACGGCAAAA GACCACGGGC TCCTGTGCGA GTACCAGTCG GCGGGGCTCT 
GGCCTGGGCA AAAGAGGAGC AGCTGAAGCT CGCCGACAGG AGAAGATGGC TGATCCTGAC 
AACAACCAGG ATGGAGTTAA CTCTTCAGCT GCACGTACAG ATGAGACTCC CCAGGGAGCT 
GCAGCTTCTA GCTCTGTTGC TGGGGCTGTA GGTATGACAA CATCTGGAGA AAGTGAGTCA 
GATGACTCTG AGATGGGAAG ACTACAAGCT CTGTTAGAGG CTAGGGGTCT TCCTCCTCAC 
CTGTTTGGCC CTCTTGGTCC TCGGATGTCG CAGCTCTTCC ACAGGACAAT TGGAAGTGGA 
GCTAGTTCTA AAGCCCAGCA GCTTTTACAA GGTCTCCAAG CCACTGATGA AAGTCAGCAA 
CTACAGGCAG TTATTGAGAT GTGCCAGCTG TTGGTCATGG GAAATGAAGA AACACTGGGA 
GGATTTCCAG TCAAGAGTGT TGTGCCAGCT TTGATAACAT TGCTGCAGAT GGAGCATAAC 
TTTGACATCA TGAACCATGC TTGTCGAGCC TTAACATACA TGATGGAAGC ACTTCCCAGA 
TCATCTGCTG TAGTGGTAGA TGCAATTCCT GTCTTCTTGG AAAAGCTGCA AGTTATTCAG 
TGCATTGATG TGGCAGAGCA GGCACTGACA GCCCTAGAGA TGCTATCACG CAGGCATAGT 
AAAGCCATTC TGCAGGCAGG TGGGTTGGCA GACTGCTTGC TGTATCTGGA ATTCTTCAGT 
ATAAATGCGC AGAGGAATGC ACTAGCTATT GCTGCCAATT GCTGCCAGAG TATAACACCT 
GATGAGTTTC ACTTTGTGGC AGACTCTTTG CCACTGCTTA CACAAAGGTT AACCCATCAG 
GACAAGAAGT CTGTTGAAAG CACTTGTCTC TGTTTTGCAC GGCTGGTAGA CAATTTCCAG 
CATGAGGAGA ACTTGCTGCA GCAGGTTGCT TCCAAGGACT TGTTAACAAA CATCCAGCAA 
CTCTTGGTAG TGACACCTCC TATCCTGAGC TCAGGAATGT TCATCATGGT GGTGCGCATG 
TTTTCCTTAA TGTGTTCCAA TTGTCCAACG CTTGCAGTCC AACTTATGAA GCAAAACATT 
GCAGAAACAC TTCACTTTCT CCTTTGTGGA GCCTCAAATG GGAGTTGTCA AGAACAAATT 
GACCTTGTTC CACGAAGTCC TCAAGAACTC TATGAGCTTA CTTCTCTTAT CTGTGAACTG 
ATGCCTTGTC TGCCAAAAGA GGGGATATTT GCTGTTGATA CTATGCTAAA GAAAGGAAAT 
GCACAAAATA CAGATGGTGC GATATGGCAA TGGCGAGATG ACAGGGGTCT CTGGCATCCC 
TATAACAGGA TTGATAGTCG AATAATAGAG GCAGCTCATC AGGTTGGTGA GGATGAGATA 
AGCCTGTCGA CACTTGGACG TGTCTATACT ATTGATTTTA ATTCTATGCA GCAAATAAAT 
GAGGATACTG GAACAGCACG TGCCATTCAG CGGAAACCAA ACCCTTTAGC CAATACAAAC 
ACTAGTGGAC ATTCAGAATT GAAGAAGGAT GATGCTCGAG CACAGCTAAT GAAAGAAGAC 
CCAGAATTGG CAAAATCCTT TATCAAAACA CTGTTTGGTG TTCTTTATGA AGTGTATAGT 
TCTTCAGCTG GACCTGCTGT TAGACACAAG TGCCTTAGAG CAATACTTAG AATAATTTAT 
TTTGCTGATG CTGAACTCCT GAAGGATGTG CTGAAAAACC ATGCTGTGTC AAGTCACATT 
GCCTCCATGC TGTCAAGTCA AGACCTTAAG ATAGTAGTTG GAGCCCTGCA GATGGCAGAA 
ATCTTAATGC AGAAGCTACC TGACATTTTT AGTGTTTACT TCAGAAGAGA AGGAGTGATG 
CATCAAGTGA AAAACTTAGC GGAGTCTGAG GCTTTGCTAA CGAGCCCACC AAAAGTATGC 
ACTAATGGAT CAGGAACACT GGGTACCACT ACAACTATAA GTACTGGAAC AGCCACTGCT 
GCCACTAATG CAGCTGCAGA TTTGGGCTCT CCCAGCTTAC AACACAGCCG GGAGGATTCT 
TTAGATCTGA GCCCACAGGG ACGACTGAGT GATGTTCTGA AGAGAAAAAG ACTGCCAAAA 
CGAGGGCCAA GGAGACCAAA ATATTCTCCC CCAAGAGATG ATGACAAAGT AGACAATCAA 
GCAAAAAGCC CCACGACTAC TCAGTCTCCT AAATCTTCTT TCTTAGCAAG TTTAAATCCC 
AAAACATGGG GAAGATTAAG TACACAGTCT AACAGTAATA ACATTGAACC AGCAAGAACA 
GCAGGTGTAA GTGGTCTTGC AAGAGCTGCT TCTAAGGATA CAATTTCCAA TAACAGAGAA 
AAAATTAAGG GTTGGATTAA GGAGCAAGCC CATAAATTTG TAGACCGTTA TTTTAGTTCG 
GAAAACATGG ATGGAAGCAA TCCTGCACTA AATGTATTAC AGAGACTTTG CACTGCAACT 
GAACAACTTA ACCTCCAGGT GGATGGTGGA ACAGAGTGCC TTGTAGAAAT CCGTAGCATT 
GTCTCGGAGT CTGACGTCTC CTCATTTGAA ATCCAGCATA GTGGATTTGT GAAACAACTG 
CTGCTTTATT TGACATCTAA AAGTGAGAAA GATGCTGTAA GCAGGGATAT CAGATTGAAA 
AGATTTCTTC ATGTATTTTT CTCCTCTCCA CTTCCTGGAG AAGAACCCCT TGGAAGATTA 
GAGCCATTAG AAAATGCACC TTTGTTGGCA TTAGTCCATA AAATGAACAA TTGCCTCAGT 
CAGATGGAGC AGTTTCCAGT GAAAGTGCAT GACTTCCCCA GTGGAAATGG AACAGGGAGC 
AGTTTTTCTC TTAACAGAGG ATCCCAAGCT TTAAAATTCT TCAATACGCA TCAGTTAAAA 
TGCCAGCTGC AAAGACATCC AGACTGTGCT AATGTGAAAC AGTGGAAGGG TGGACCTGTG 
AAGATTGATC CTCTGGCTTT GGTTCAAGCC ATTGAAAGAT ATCTTGTTGT TAGAGGTTAT 
GGAAGAGTTA GAGAAGATGA TGAGGATAGT GATGATGATG GGTCAGATGA AGAAATAGAT 
GAATCCTTGG CTGCTCAATT CTTAAATTCA GGGAATGTGA GACATAGATT GCAGTTTTAC 
ATTGGGGATC ACTTGCTACC ATATAATATG ACTGTATATC AAGCAGTTAA GCTGTACAGT 
TTGCAAGCTG AGGAGGAGAG GGAATCGACT GATGATGAAA GCAATCCATT AGGAAGAGCT 
GGGATTTGGA CAAAAACACA TACCATTTGG TACAAACCTG TACGGGAGGA TGAAGATGGC 
AACAAAGACT GTGTTGGTGG TAAAAGAGGA AGAGCTCAAA CTGCTCCCAC AAAAACCTCA 
CCTAGGAATT CTAAAAAGCA TGATGAGTTG TGGCACGATG GTGTATGCCC TTCAGTATTG 
AATCCTTTAG AAGTTTACCT CATATCTACT CCTCCTGAAA ACATAACCTT TGAAGATCCT 
TCATTAGATG TTATTCTTCT TTTGAGAGTT TTACATGCTA TCAGTCGATA CTGGTATTAT 
TTGTATGATA ATGCAATATG CAAGGAGATT ATTCCAACTT CGGAATTTAT CAATAGTAAA 
CTGACAGCAA AAGCAAACAG ACAGCTTCAA GATCCACTGG TAATTATGAC AGGAAACATA 
CCGACTTGGC TGACAGAGCT TGGAAAAACA TGCCCATTTT TCTTTCCATT TGATACCCGG 
CAAATGCTGT TTTATGTAAC TGCTTTTGAT CGTGATCGAG CCATGCAAAG ACTACTGGAT 
ACTAATCCAG AAATCAATCA ATCAGATTCT CAGGATAGCA GAGTGGCACC ACGACTGGAC 
AGGAAAAAAC GCACTGTGAA CAGAGATGAG CTGTTGAAAC AGGCAGAATC TGTGATGCAG 
GATCTAGGCA GTTCAAGAGC CATGTTGGAA ATCCAGTATG AGAATGAAGT TGGCACAGGC 
CTGGGCCCTA CACTAGAGTT CTATGCACTT GTATCTCAGG AACTACAGAG AGCAGACTTG 
GGCCTTTGGA GGGGAGAAGA AGTAACTTTA GCCAATCCAA AAGGAAGCCA AGAAGGTACC 
AAGTATATTC ATAATCTCCA AGGCCTTTTT GCACTTCCTT TTGGTAGAAC AGCCAAGCCT 
GCTCACATTG CAAAAGTTAA AATGAAGTTC CGCTTTCTGG GAAAACTAAT GGCTAAGGCA 
ATCATGGATT TTAGACTGGT GGATCTTCCT CTTGGACTTC CTTTTTATAA ATGGATGTTA 
CGCCAGGAAA CTTCTTTGAC GTCACATGAC TTGTTCAGTA TTGATCCAGT AGTAGCCAAA 
TCAATATATC ACCTTGAAGA TATTGTAAGA CAAAAGAAAA GACTTGAGCA GGACAAAACA 
CAGACCAAAG AAAGTCTACA GTATGCATTG GAAACCCTGA CTATGAATGG CTGCTCAGTG 
GAGGATCTAG GGCTGGATTT CACACTTCCT GGATTTCCTA ACATCGAGCT GAAAAAAGGG 
GGAAAAGATA CACCAGTCAC CATCCACAAC TTGGAGGAGT ACCTCAGATT GGTTATATTC 
TGGGCACTTA ACGAAGGTGT TGCCAGACAG TTTGATTCTT TCAGAGATGG ATTTGAATCA 
GTCTTCCCCC TCAGTCATCT TCAATACTTT TATCCTGAGG AGTTGGAGCA ACTCTTATGT 
GGCAGCAAAA CAGACACATG GGATGCCAAG ACCTTAATGG AATGTTGCAG GCCAGATCAC 
GGTTATACAC ATGACAGTCG AGCAGTGAAG TATCTCTTTG AAATTCTCAG TAGTTTTGAT 
AGTGAGCAGC AGAGACTGTT TCTTCAGTTT GTGACCGGTA GTCCCAGGCT GCCAGTAGGA 
GGCTTTCGAA GTTTGAATCC TCCATTGACA ATTGTACGGA AGACATTTGA GTCTACAGAG 
AATCCAGATG ACTTCTTGCC CTCAGTAATG ACTTGTGTGA ACTATCTCAA ATTGCCGGAC 
TATTCAACTA TTGAGATAAT GCGTGAAAAA CTCTTGATAG CTGCAAGAGA AGGGCAGCAG 
TCATTCCATC TTTCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TRIP12 ( XM_004937065.3 ) cDNA ORF clone, Gallus gallus(chicken) -> XP_004937122.1 Gallus gallus thyroid hormone receptor interactor 12 (TRIP12), transcript variant X6, mRNA.

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CloneID	OGa12503
Clone ID Related Accession (Same CDS sequence)	XM_004937065.3 , XM_004937065.2
Accession Version	XM_004937065.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6183bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-16
Organism	Gallus gallus(chicken)
Product	E3 ubiquitin-protein ligase TRIP12 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006096.5) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On May 17, 2018 this sequence version replaced XM_004937065.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Gallus gallus Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCCAACC GGCCTAATAA CAATCCAGGG GGGTCACTGC GACGTTCACA GAGGAACACT 
GCTGGGGCCC AGCCACAAGA CGACGCAGCA GGAGGAAGGT CACATTTAGG GCAGGCAAAA 
CATAAGGCAC ATAGCCCTCC TGAGAGTAGA AAATCTATTT CAAAGACACC CAAAGTGCAG 
TCTAATACTA CTTCTGAGCA GTCCAAGGGA CACTTTTCTA AAAGAGGCTG TAGTTCATCT 
GCTGTTTTAA TACCACAAGA AGATTCAGAA AGAGTCAGTA CTTCAGAAAA GCAAAAAACG 
GGGCAAGTGC CTAAGAAAGA CAATTCTCGA GGAGTTAAAC GCAGTGCTAG TCCAGATTAC 
AGCAGGACCA ATTCTCCTAG CTCTGCTAAA AAACCTAAAG CACTTCAACA CGCCGAAACT 
TCCTCGGAAA GTAACAAGCC ACATACTAAA TCCAAGAGAA GACACTTAGA CCAAGAACAG 
CAGAAGTCTA CACAATTGCC ATCAACAAGC AAGGCTCACA CCAGAAAGGG TGGAGCTGCT 
GGTAGCTCCC GAAGTCAGAA AAGGAAAAGG ACAGAGAGTA CGTCTTGTAT AAAGAGTCGT 
TCAGCAGTTG AATCAACTGG AGCTGAAGAG AAGTCAGCAA AATCCTCCAA GCTGGCTTCA 
AAATCGGTGA CCTCAGCCAA AGCTGGGTGT AGCACCATCA CTGATTCTTC TTCTTCAGCT 
TCTACATCCT CTTCCTCTTC TGCTGTTGCC TCTGCTTCTT CCACTGTTCC TCAGGGTGCC 
AGAGTGAAAC AGGGTAAGGA CCAGAATAAG GCTAGACGTT CACGTTCTGC GTCCAGCCCT 
AGTCCAAGAA GGAGCAGCAG GGACAAAGAA CAGAGCAAAA CAGGTGGCTC TTCAAAGTTT 
GACTGGGCTG CTCGATTCAG TCCAAAAGTT AGTCTCCCTA AAACAAAACT GTCTCTACCA 
GGCTCTTCTA AGTCAGAGAC CTCAAAACCT GGACCTTCAG GACTACAGGC TAAGCTAGCA 
AGCCTAAGAA AATCCACAAA GAAGCGCAGT GAATCACCAC CTGCTGAGCT CCCCAGTTTA 
CGGCGGAGCA CACGGCAAAA GACCACGGGC TCCTGTGCGA GTACCAGTCG GCGGGGCTCT 
GGCCTGGGCA AAAGAGGAGC AGCTGAAGCT CGCCGACAGG AGAAGATGGC TGATCCTGAC 
AACAACCAGG ATGGAGTTAA CTCTTCAGCT GCACGTACAG ATGAGACTCC CCAGGGAGCT 
GCAGCTTCTA GCTCTGTTGC TGGGGCTGTA GGTATGACAA CATCTGGAGA AAGTGAGTCA 
GATGACTCTG AGATGGGAAG ACTACAAGCT CTGTTAGAGG CTAGGGGTCT TCCTCCTCAC 
CTGTTTGGCC CTCTTGGTCC TCGGATGTCG CAGCTCTTCC ACAGGACAAT TGGAAGTGGA 
GCTAGTTCTA AAGCCCAGCA GCTTTTACAA GGTCTCCAAG CCACTGATGA AAGTCAGCAA 
CTACAGGCAG TTATTGAGAT GTGCCAGCTG TTGGTCATGG GAAATGAAGA AACACTGGGA 
GGATTTCCAG TCAAGAGTGT TGTGCCAGCT TTGATAACAT TGCTGCAGAT GGAGCATAAC 
TTTGACATCA TGAACCATGC TTGTCGAGCC TTAACATACA TGATGGAAGC ACTTCCCAGA 
TCATCTGCTG TAGTGGTAGA TGCAATTCCT GTCTTCTTGG AAAAGCTGCA AGTTATTCAG 
TGCATTGATG TGGCAGAGCA GGCACTGACA GCCCTAGAGA TGCTATCACG CAGGCATAGT 
AAAGCCATTC TGCAGGCAGG TGGGTTGGCA GACTGCTTGC TGTATCTGGA ATTCTTCAGT 
ATAAATGCGC AGAGGAATGC ACTAGCTATT GCTGCCAATT GCTGCCAGAG TATAACACCT 
GATGAGTTTC ACTTTGTGGC AGACTCTTTG CCACTGCTTA CACAAAGGTT AACCCATCAG 
GACAAGAAGT CTGTTGAAAG CACTTGTCTC TGTTTTGCAC GGCTGGTAGA CAATTTCCAG 
CATGAGGAGA ACTTGCTGCA GCAGGTTGCT TCCAAGGACT TGTTAACAAA CATCCAGCAA 
CTCTTGGTAG TGACACCTCC TATCCTGAGC TCAGGAATGT TCATCATGGT GGTGCGCATG 
TTTTCCTTAA TGTGTTCCAA TTGTCCAACG CTTGCAGTCC AACTTATGAA GCAAAACATT 
GCAGAAACAC TTCACTTTCT CCTTTGTGGA GCCTCAAATG GGAGTTGTCA AGAACAAATT 
GACCTTGTTC CACGAAGTCC TCAAGAACTC TATGAGCTTA CTTCTCTTAT CTGTGAACTG 
ATGCCTTGTC TGCCAAAAGA GGGGATATTT GCTGTTGATA CTATGCTAAA GAAAGGAAAT 
GCACAAAATA CAGATGGTGC GATATGGCAA TGGCGAGATG ACAGGGGTCT CTGGCATCCC 
TATAACAGGA TTGATAGTCG AATAATAGAG GCAGCTCATC AGGTTGGTGA GGATGAGATA 
AGCCTGTCGA CACTTGGACG TGTCTATACT ATTGATTTTA ATTCTATGCA GCAAATAAAT 
GAGGATACTG GAACAGCACG TGCCATTCAG CGGAAACCAA ACCCTTTAGC CAATACAAAC 
ACTAGTGGAC ATTCAGAATT GAAGAAGGAT GATGCTCGAG CACAGCTAAT GAAAGAAGAC 
CCAGAATTGG CAAAATCCTT TATCAAAACA CTGTTTGGTG TTCTTTATGA AGTGTATAGT 
TCTTCAGCTG GACCTGCTGT TAGACACAAG TGCCTTAGAG CAATACTTAG AATAATTTAT 
TTTGCTGATG CTGAACTCCT GAAGGATGTG CTGAAAAACC ATGCTGTGTC AAGTCACATT 
GCCTCCATGC TGTCAAGTCA AGACCTTAAG ATAGTAGTTG GAGCCCTGCA GATGGCAGAA 
ATCTTAATGC AGAAGCTACC TGACATTTTT AGTGTTTACT TCAGAAGAGA AGGAGTGATG 
CATCAAGTGA AAAACTTAGC GGAGTCTGAG GCTTTGCTAA CGAGCCCACC AAAAGTATGC 
ACTAATGGAT CAGGAACACT GGGTACCACT ACAACTATAA GTACTGGAAC AGCCACTGCT 
GCCACTAATG CAGCTGCAGA TTTGGGCTCT CCCAGCTTAC AACACAGCCG GGAGGATTCT 
TTAGATCTGA GCCCACAGGG ACGACTGAGT GATGTTCTGA AGAGAAAAAG ACTGCCAAAA 
CGAGGGCCAA GGAGACCAAA ATATTCTCCC CCAAGAGATG ATGACAAAGT AGACAATCAA 
GCAAAAAGCC CCACGACTAC TCAGTCTCCT AAATCTTCTT TCTTAGCAAG TTTAAATCCC 
AAAACATGGG GAAGATTAAG TACACAGTCT AACAGTAATA ACATTGAACC AGCAAGAACA 
GCAGGTGTAA GTGGTCTTGC AAGAGCTGCT TCTAAGGATA CAATTTCCAA TAACAGAGAA 
AAAATTAAGG GTTGGATTAA GGAGCAAGCC CATAAATTTG TAGACCGTTA TTTTAGTTCG 
GAAAACATGG ATGGAAGCAA TCCTGCACTA AATGTATTAC AGAGACTTTG CACTGCAACT 
GAACAACTTA ACCTCCAGGT GGATGGTGGA ACAGAGTGCC TTGTAGAAAT CCGTAGCATT 
GTCTCGGAGT CTGACGTCTC CTCATTTGAA ATCCAGCATA GTGGATTTGT GAAACAACTG 
CTGCTTTATT TGACATCTAA AAGTGAGAAA GATGCTGTAA GCAGGGATAT CAGATTGAAA 
AGATTTCTTC ATGTATTTTT CTCCTCTCCA CTTCCTGGAG AAGAACCCCT TGGAAGATTA 
GAGCCATTAG AAAATGCACC TTTGTTGGCA TTAGTCCATA AAATGAACAA TTGCCTCAGT 
CAGATGGAGC AGTTTCCAGT GAAAGTGCAT GACTTCCCCA GTGGAAATGG AACAGGGAGC 
AGAGGATCCC AAGCTTTAAA ATTCTTCAAT ACGCATCAGT TAAAATGCCA GCTGCAAAGA 
CATCCAGACT GTGCTAATGT GAAACAGTGG AAGGGTGGAC CTGTGAAGAT TGATCCTCTG 
GCTTTGGTTC AAGCCATTGA AAGATATCTT GTTGTTAGAG GTTATGGAAG AGTTAGAGAA 
GATGATGAGG ATAGTGATGA TGATGGGTCA GATGAAGAAA TAGATGAATC CTTGGCTGCT 
CAATTCTTAA ATTCAGGGAA TGTGAGACAT AGATTGCAGT TTTACATTGG GGATCACTTG 
CTACCATATA ATATGACTGT ATATCAAGCA GTTAAGCTGT ACAGTTTGCA AGCTGAGGAG 
GAGAGGGAAT CGACTGATGA TGAAAGCAAT CCATTAGGAA GAGCTGGGAT TTGGACAAAA 
ACACATACCA TTTGGTACAA ACCTGTACGG GAGGATGAAG ATGGCAACAA AGACTGTGTT 
GGTGGTAAAA GAGGAAGAGC TCAAACTGCT CCCACAAAAA CCTCACCTAG GAATTCTAAA 
AAGCATGATG AGTTGTGGCA CGATGGTGTA TGCCCTTCAG TATTGAATCC TTTAGAAGTT 
TACCTCATAT CTACTCCTCC TGAAAACATA ACCTTTGAAG ATCCTTCATT AGATGTTATT 
CTTCTTTTGA GAGTTTTACA TGCTATCAGT CGATACTGGT ATTATTTGTA TGATAATGCA 
ATATGCAAGG AGATTATTCC AACTTCGGAA TTTATCAATA GTAAACTGAC AGCAAAAGCA 
AACAGACAGC TTCAAGATCC ACTGGTAATT ATGACAGGAA ACATACCGAC TTGGCTGACA 
GAGCTTGGAA AAACATGCCC ATTTTTCTTT CCATTTGATA CCCGGCAAAT GCTGTTTTAT 
GTAACTGCTT TTGATCGTGA TCGAGCCATG CAAAGACTAC TGGATACTAA TCCAGAAATC 
AATCAATCAG ATTCTCAGGA TAGCAGAGTG GCACCACGAC TGGACAGGAA AAAACGCACT 
GTGAACAGAG ATGAGCTGTT GAAACAGGCA GAATCTGTGA TGCAGGATCT AGGCAGTTCA 
AGAGCCATGT TGGAAATCCA GTATGAGAAT GAAGTTGGCA CAGGCCTGGG CCCTACACTA 
GAGTTCTATG CACTTGTATC TCAGGAACTA CAGAGAGCAG ACTTGGGCCT TTGGAGGGGA 
GAAGAAGTAA CTTTAGCCAA TCCAAAAGGA AGCCAAGAAG GTACCAAGTA TATTCATAAT 
CTCCAAGGCC TTTTTGCACT TCCTTTTGGT AGAACAGCCA AGCCTGCTCA CATTGCAAAA 
GTTAAAATGA AGTTCCGCTT TCTGGGAAAA CTAATGGCTA AGGCAATCAT GGATTTTAGA 
CTGGTGGATC TTCCTCTTGG ACTTCCTTTT TATAAATGGA TGTTACGCCA GGAAACTTCT 
TTGACGTCAC ATGACTTGTT CAGTATTGAT CCAGTAGTAG CCAAATCAAT ATATCACCTT 
GAAGATATTG TAAGACAAAA GAAAAGACTT GAGCAGGACA AAACACAGAC CAAAGAAAGT 
CTACAGTATG CATTGGAAAC CCTGACTATG AATGGCTGCT CAGTGGAGGA TCTAGGGCTG 
GATTTCACAC TTCCTGGATT TCCTAACATC GAGCTGAAAA AAGGGGGAAA AGATACACCA 
GTCACCATCC ACAACTTGGA GGAGTACCTC AGATTGGTTA TATTCTGGGC ACTTAACGAA 
GGTGTTGCCA GACAGTTTGA TTCTTTCAGA GATGGATTTG AATCAGTCTT CCCCCTCAGT 
CATCTTCAAT ACTTTTATCC TGAGGAGTTG GAGCAACTCT TATGTGGCAG CAAAACAGAC 
ACATGGGATG CCAAGACCTT AATGGAATGT TGCAGGCCAG ATCACGGTTA TACACATGAC 
AGTCGAGCAG TGAAGTATCT CTTTGAAATT CTCAGTAGTT TTGATAGTGA GCAGCAGAGA 
CTGTTTCTTC AGTTTGTGAC CGGTAGTCCC AGGCTGCCAG TAGGAGGCTT TCGAAGTTTG 
AATCCTCCAT TGACAATTGT ACGGAAGACA TTTGAGTCTA CAGAGAATCC AGATGACTTC 
TTGCCCTCAG TAATGACTTG TGTGAACTAT CTCAAATTGC CGGACTATTC AACTATTGAG 
ATAATGCGTG AAAAACTCTT GATAGCTGCA AGAGAAGGGC AGCAGTCATT CCATCTTTCC 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_004937122.1
CDS	134..6316
Translation	MSNRPNNNPGGSLRRSQRNTAGAQPQDDAAGGRSHLGQAKHKAHSPPESRKSISKTPKVQSNTTSEQSKGHFSKRGCSSSAVLIPQEDSERVSTSEKQKTGQVPKKDNSRGVKRSASPDYSRTNSPSSAKKPKALQHAETSSESNKPHTKSKRRHLDQEQQKSTQLPSTSKAHTRKGGAAGSSRSQKRKRTESTSCIKSRSAVESTGAEEKSAKSSKLASKSVTSAKAGCSTITDSSSSASTSSSSSAVASASSTVPQGARVKQGKDQNKARRSRSASSPSPRRSSRDKEQSKTGGSSKFDWAARFSPKVSLPKTKLSLPGSSKSETSKPGPSGLQAKLASLRKSTKKRSESPPAELPSLRRSTRQKTTGSCASTSRRGSGLGKRGAAEARRQEKMADPDNNQDGVNSSAARTDETPQGAAASSSVAGAVGMTTSGESESDDSEMGRLQALLEARGLPPHLFGPLGPRMSQLFHRTIGSGASSKAQQLLQGLQATDESQQLQAVIEMCQLLVMGNEETLGGFPVKSVVPALITLLQMEHNFDIMNHACRALTYMMEALPRSSAVVVDAIPVFLEKLQVIQCIDVAEQALTALEMLSRRHSKAILQAGGLADCLLYLEFFSINAQRNALAIAANCCQSITPDEFHFVADSLPLLTQRLTHQDKKSVESTCLCFARLVDNFQHEENLLQQVASKDLLTNIQQLLVVTPPILSSGMFIMVVRMFSLMCSNCPTLAVQLMKQNIAETLHFLLCGASNGSCQEQIDLVPRSPQELYELTSLICELMPCLPKEGIFAVDTMLKKGNAQNTDGAIWQWRDDRGLWHPYNRIDSRIIEAAHQVGEDEISLSTLGRVYTIDFNSMQQINEDTGTARAIQRKPNPLANTNTSGHSELKKDDARAQLMKEDPELAKSFIKTLFGVLYEVYSSSAGPAVRHKCLRAILRIIYFADAELLKDVLKNHAVSSHIASMLSSQDLKIVVGALQMAEILMQKLPDIFSVYFRREGVMHQVKNLAESEALLTSPPKVCTNGSGTLGTTTTISTGTATAATNAAADLGSPSLQHSREDSLDLSPQGRLSDVLKRKRLPKRGPRRPKYSPPRDDDKVDNQAKSPTTTQSPKSSFLASLNPKTWGRLSTQSNSNNIEPARTAGVSGLARAASKDTISNNREKIKGWIKEQAHKFVDRYFSSENMDGSNPALNVLQRLCTATEQLNLQVDGGTECLVEIRSIVSESDVSSFEIQHSGFVKQLLLYLTSKSEKDAVSRDIRLKRFLHVFFSSPLPGEEPLGRLEPLENAPLLALVHKMNNCLSQMEQFPVKVHDFPSGNGTGSRGSQALKFFNTHQLKCQLQRHPDCANVKQWKGGPVKIDPLALVQAIERYLVVRGYGRVREDDEDSDDDGSDEEIDESLAAQFLNSGNVRHRLQFYIGDHLLPYNMTVYQAVKLYSLQAEEERESTDDESNPLGRAGIWTKTHTIWYKPVREDEDGNKDCVGGKRGRAQTAPTKTSPRNSKKHDELWHDGVCPSVLNPLEVYLISTPPENITFEDPSLDVILLLRVLHAISRYWYYLYDNAICKEIIPTSEFINSKLTAKANRQLQDPLVIMTGNIPTWLTELGKTCPFFFPFDTRQMLFYVTAFDRDRAMQRLLDTNPEINQSDSQDSRVAPRLDRKKRTVNRDELLKQAESVMQDLGSSRAMLEIQYENEVGTGLGPTLEFYALVSQELQRADLGLWRGEEVTLANPKGSQEGTKYIHNLQGLFALPFGRTAKPAHIAKVKMKFRFLGKLMAKAIMDFRLVDLPLGLPFYKWMLRQETSLTSHDLFSIDPVVAKSIYHLEDIVRQKKRLEQDKTQTKESLQYALETLTMNGCSVEDLGLDFTLPGFPNIELKKGGKDTPVTIHNLEEYLRLVIFWALNEGVARQFDSFRDGFESVFPLSHLQYFYPEELEQLLCGSKTDTWDAKTLMECCRPDHGYTHDSRAVKYLFEILSSFDSEQQRLFLQFVTGSPRLPVGGFRSLNPPLTIVRKTFESTENPDDFLPSVMTCVNYLKLPDYSTIEIMREKLLIAAREGQQSFHLS

Target ORF information:

RefSeq Version	XM_004937065.3
Organism	Gallus gallus(chicken)
Definition	Gallus gallus thyroid hormone receptor interactor 12 (TRIP12), transcript variant X6, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_004937065.3

ORF Insert Sequence:

ATGTCCAACC GGCCTAATAA CAATCCAGGG GGGTCACTGC GACGTTCACA GAGGAACACT 
GCTGGGGCCC AGCCACAAGA CGACGCAGCA GGAGGAAGGT CACATTTAGG GCAGGCAAAA 
CATAAGGCAC ATAGCCCTCC TGAGAGTAGA AAATCTATTT CAAAGACACC CAAAGTGCAG 
TCTAATACTA CTTCTGAGCA GTCCAAGGGA CACTTTTCTA AAAGAGGCTG TAGTTCATCT 
GCTGTTTTAA TACCACAAGA AGATTCAGAA AGAGTCAGTA CTTCAGAAAA GCAAAAAACG 
GGGCAAGTGC CTAAGAAAGA CAATTCTCGA GGAGTTAAAC GCAGTGCTAG TCCAGATTAC 
AGCAGGACCA ATTCTCCTAG CTCTGCTAAA AAACCTAAAG CACTTCAACA CGCCGAAACT 
TCCTCGGAAA GTAACAAGCC ACATACTAAA TCCAAGAGAA GACACTTAGA CCAAGAACAG 
CAGAAGTCTA CACAATTGCC ATCAACAAGC AAGGCTCACA CCAGAAAGGG TGGAGCTGCT 
GGTAGCTCCC GAAGTCAGAA AAGGAAAAGG ACAGAGAGTA CGTCTTGTAT AAAGAGTCGT 
TCAGCAGTTG AATCAACTGG AGCTGAAGAG AAGTCAGCAA AATCCTCCAA GCTGGCTTCA 
AAATCGGTGA CCTCAGCCAA AGCTGGGTGT AGCACCATCA CTGATTCTTC TTCTTCAGCT 
TCTACATCCT CTTCCTCTTC TGCTGTTGCC TCTGCTTCTT CCACTGTTCC TCAGGGTGCC 
AGAGTGAAAC AGGGTAAGGA CCAGAATAAG GCTAGACGTT CACGTTCTGC GTCCAGCCCT 
AGTCCAAGAA GGAGCAGCAG GGACAAAGAA CAGAGCAAAA CAGGTGGCTC TTCAAAGTTT 
GACTGGGCTG CTCGATTCAG TCCAAAAGTT AGTCTCCCTA AAACAAAACT GTCTCTACCA 
GGCTCTTCTA AGTCAGAGAC CTCAAAACCT GGACCTTCAG GACTACAGGC TAAGCTAGCA 
AGCCTAAGAA AATCCACAAA GAAGCGCAGT GAATCACCAC CTGCTGAGCT CCCCAGTTTA 
CGGCGGAGCA CACGGCAAAA GACCACGGGC TCCTGTGCGA GTACCAGTCG GCGGGGCTCT 
GGCCTGGGCA AAAGAGGAGC AGCTGAAGCT CGCCGACAGG AGAAGATGGC TGATCCTGAC 
AACAACCAGG ATGGAGTTAA CTCTTCAGCT GCACGTACAG ATGAGACTCC CCAGGGAGCT 
GCAGCTTCTA GCTCTGTTGC TGGGGCTGTA GGTATGACAA CATCTGGAGA AAGTGAGTCA 
GATGACTCTG AGATGGGAAG ACTACAAGCT CTGTTAGAGG CTAGGGGTCT TCCTCCTCAC 
CTGTTTGGCC CTCTTGGTCC TCGGATGTCG CAGCTCTTCC ACAGGACAAT TGGAAGTGGA 
GCTAGTTCTA AAGCCCAGCA GCTTTTACAA GGTCTCCAAG CCACTGATGA AAGTCAGCAA 
CTACAGGCAG TTATTGAGAT GTGCCAGCTG TTGGTCATGG GAAATGAAGA AACACTGGGA 
GGATTTCCAG TCAAGAGTGT TGTGCCAGCT TTGATAACAT TGCTGCAGAT GGAGCATAAC 
TTTGACATCA TGAACCATGC TTGTCGAGCC TTAACATACA TGATGGAAGC ACTTCCCAGA 
TCATCTGCTG TAGTGGTAGA TGCAATTCCT GTCTTCTTGG AAAAGCTGCA AGTTATTCAG 
TGCATTGATG TGGCAGAGCA GGCACTGACA GCCCTAGAGA TGCTATCACG CAGGCATAGT 
AAAGCCATTC TGCAGGCAGG TGGGTTGGCA GACTGCTTGC TGTATCTGGA ATTCTTCAGT 
ATAAATGCGC AGAGGAATGC ACTAGCTATT GCTGCCAATT GCTGCCAGAG TATAACACCT 
GATGAGTTTC ACTTTGTGGC AGACTCTTTG CCACTGCTTA CACAAAGGTT AACCCATCAG 
GACAAGAAGT CTGTTGAAAG CACTTGTCTC TGTTTTGCAC GGCTGGTAGA CAATTTCCAG 
CATGAGGAGA ACTTGCTGCA GCAGGTTGCT TCCAAGGACT TGTTAACAAA CATCCAGCAA 
CTCTTGGTAG TGACACCTCC TATCCTGAGC TCAGGAATGT TCATCATGGT GGTGCGCATG 
TTTTCCTTAA TGTGTTCCAA TTGTCCAACG CTTGCAGTCC AACTTATGAA GCAAAACATT 
GCAGAAACAC TTCACTTTCT CCTTTGTGGA GCCTCAAATG GGAGTTGTCA AGAACAAATT 
GACCTTGTTC CACGAAGTCC TCAAGAACTC TATGAGCTTA CTTCTCTTAT CTGTGAACTG 
ATGCCTTGTC TGCCAAAAGA GGGGATATTT GCTGTTGATA CTATGCTAAA GAAAGGAAAT 
GCACAAAATA CAGATGGTGC GATATGGCAA TGGCGAGATG ACAGGGGTCT CTGGCATCCC 
TATAACAGGA TTGATAGTCG AATAATAGAG GCAGCTCATC AGGTTGGTGA GGATGAGATA 
AGCCTGTCGA CACTTGGACG TGTCTATACT ATTGATTTTA ATTCTATGCA GCAAATAAAT 
GAGGATACTG GAACAGCACG TGCCATTCAG CGGAAACCAA ACCCTTTAGC CAATACAAAC 
ACTAGTGGAC ATTCAGAATT GAAGAAGGAT GATGCTCGAG CACAGCTAAT GAAAGAAGAC 
CCAGAATTGG CAAAATCCTT TATCAAAACA CTGTTTGGTG TTCTTTATGA AGTGTATAGT 
TCTTCAGCTG GACCTGCTGT TAGACACAAG TGCCTTAGAG CAATACTTAG AATAATTTAT 
TTTGCTGATG CTGAACTCCT GAAGGATGTG CTGAAAAACC ATGCTGTGTC AAGTCACATT 
GCCTCCATGC TGTCAAGTCA AGACCTTAAG ATAGTAGTTG GAGCCCTGCA GATGGCAGAA 
ATCTTAATGC AGAAGCTACC TGACATTTTT AGTGTTTACT TCAGAAGAGA AGGAGTGATG 
CATCAAGTGA AAAACTTAGC GGAGTCTGAG GCTTTGCTAA CGAGCCCACC AAAAGTATGC 
ACTAATGGAT CAGGAACACT GGGTACCACT ACAACTATAA GTACTGGAAC AGCCACTGCT 
GCCACTAATG CAGCTGCAGA TTTGGGCTCT CCCAGCTTAC AACACAGCCG GGAGGATTCT 
TTAGATCTGA GCCCACAGGG ACGACTGAGT GATGTTCTGA AGAGAAAAAG ACTGCCAAAA 
CGAGGGCCAA GGAGACCAAA ATATTCTCCC CCAAGAGATG ATGACAAAGT AGACAATCAA 
GCAAAAAGCC CCACGACTAC TCAGTCTCCT AAATCTTCTT TCTTAGCAAG TTTAAATCCC 
AAAACATGGG GAAGATTAAG TACACAGTCT AACAGTAATA ACATTGAACC AGCAAGAACA 
GCAGGTGTAA GTGGTCTTGC AAGAGCTGCT TCTAAGGATA CAATTTCCAA TAACAGAGAA 
AAAATTAAGG GTTGGATTAA GGAGCAAGCC CATAAATTTG TAGACCGTTA TTTTAGTTCG 
GAAAACATGG ATGGAAGCAA TCCTGCACTA AATGTATTAC AGAGACTTTG CACTGCAACT 
GAACAACTTA ACCTCCAGGT GGATGGTGGA ACAGAGTGCC TTGTAGAAAT CCGTAGCATT 
GTCTCGGAGT CTGACGTCTC CTCATTTGAA ATCCAGCATA GTGGATTTGT GAAACAACTG 
CTGCTTTATT TGACATCTAA AAGTGAGAAA GATGCTGTAA GCAGGGATAT CAGATTGAAA 
AGATTTCTTC ATGTATTTTT CTCCTCTCCA CTTCCTGGAG AAGAACCCCT TGGAAGATTA 
GAGCCATTAG AAAATGCACC TTTGTTGGCA TTAGTCCATA AAATGAACAA TTGCCTCAGT 
CAGATGGAGC AGTTTCCAGT GAAAGTGCAT GACTTCCCCA GTGGAAATGG AACAGGGAGC 
AGAGGATCCC AAGCTTTAAA ATTCTTCAAT ACGCATCAGT TAAAATGCCA GCTGCAAAGA 
CATCCAGACT GTGCTAATGT GAAACAGTGG AAGGGTGGAC CTGTGAAGAT TGATCCTCTG 
GCTTTGGTTC AAGCCATTGA AAGATATCTT GTTGTTAGAG GTTATGGAAG AGTTAGAGAA 
GATGATGAGG ATAGTGATGA TGATGGGTCA GATGAAGAAA TAGATGAATC CTTGGCTGCT 
CAATTCTTAA ATTCAGGGAA TGTGAGACAT AGATTGCAGT TTTACATTGG GGATCACTTG 
CTACCATATA ATATGACTGT ATATCAAGCA GTTAAGCTGT ACAGTTTGCA AGCTGAGGAG 
GAGAGGGAAT CGACTGATGA TGAAAGCAAT CCATTAGGAA GAGCTGGGAT TTGGACAAAA 
ACACATACCA TTTGGTACAA ACCTGTACGG GAGGATGAAG ATGGCAACAA AGACTGTGTT 
GGTGGTAAAA GAGGAAGAGC TCAAACTGCT CCCACAAAAA CCTCACCTAG GAATTCTAAA 
AAGCATGATG AGTTGTGGCA CGATGGTGTA TGCCCTTCAG TATTGAATCC TTTAGAAGTT 
TACCTCATAT CTACTCCTCC TGAAAACATA ACCTTTGAAG ATCCTTCATT AGATGTTATT 
CTTCTTTTGA GAGTTTTACA TGCTATCAGT CGATACTGGT ATTATTTGTA TGATAATGCA 
ATATGCAAGG AGATTATTCC AACTTCGGAA TTTATCAATA GTAAACTGAC AGCAAAAGCA 
AACAGACAGC TTCAAGATCC ACTGGTAATT ATGACAGGAA ACATACCGAC TTGGCTGACA 
GAGCTTGGAA AAACATGCCC ATTTTTCTTT CCATTTGATA CCCGGCAAAT GCTGTTTTAT 
GTAACTGCTT TTGATCGTGA TCGAGCCATG CAAAGACTAC TGGATACTAA TCCAGAAATC 
AATCAATCAG ATTCTCAGGA TAGCAGAGTG GCACCACGAC TGGACAGGAA AAAACGCACT 
GTGAACAGAG ATGAGCTGTT GAAACAGGCA GAATCTGTGA TGCAGGATCT AGGCAGTTCA 
AGAGCCATGT TGGAAATCCA GTATGAGAAT GAAGTTGGCA CAGGCCTGGG CCCTACACTA 
GAGTTCTATG CACTTGTATC TCAGGAACTA CAGAGAGCAG ACTTGGGCCT TTGGAGGGGA 
GAAGAAGTAA CTTTAGCCAA TCCAAAAGGA AGCCAAGAAG GTACCAAGTA TATTCATAAT 
CTCCAAGGCC TTTTTGCACT TCCTTTTGGT AGAACAGCCA AGCCTGCTCA CATTGCAAAA 
GTTAAAATGA AGTTCCGCTT TCTGGGAAAA CTAATGGCTA AGGCAATCAT GGATTTTAGA 
CTGGTGGATC TTCCTCTTGG ACTTCCTTTT TATAAATGGA TGTTACGCCA GGAAACTTCT 
TTGACGTCAC ATGACTTGTT CAGTATTGAT CCAGTAGTAG CCAAATCAAT ATATCACCTT 
GAAGATATTG TAAGACAAAA GAAAAGACTT GAGCAGGACA AAACACAGAC CAAAGAAAGT 
CTACAGTATG CATTGGAAAC CCTGACTATG AATGGCTGCT CAGTGGAGGA TCTAGGGCTG 
GATTTCACAC TTCCTGGATT TCCTAACATC GAGCTGAAAA AAGGGGGAAA AGATACACCA 
GTCACCATCC ACAACTTGGA GGAGTACCTC AGATTGGTTA TATTCTGGGC ACTTAACGAA 
GGTGTTGCCA GACAGTTTGA TTCTTTCAGA GATGGATTTG AATCAGTCTT CCCCCTCAGT 
CATCTTCAAT ACTTTTATCC TGAGGAGTTG GAGCAACTCT TATGTGGCAG CAAAACAGAC 
ACATGGGATG CCAAGACCTT AATGGAATGT TGCAGGCCAG ATCACGGTTA TACACATGAC 
AGTCGAGCAG TGAAGTATCT CTTTGAAATT CTCAGTAGTT TTGATAGTGA GCAGCAGAGA 
CTGTTTCTTC AGTTTGTGAC CGGTAGTCCC AGGCTGCCAG TAGGAGGCTT TCGAAGTTTG 
AATCCTCCAT TGACAATTGT ACGGAAGACA TTTGAGTCTA CAGAGAATCC AGATGACTTC 
TTGCCCTCAG TAATGACTTG TGTGAACTAT CTCAAATTGC CGGACTATTC AACTATTGAG 
ATAATGCGTG AAAAACTCTT GATAGCTGCA AGAGAAGGGC AGCAGTCATT CCATCTTTCC 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TRIP12 ( XM_004937065.2 ) cDNA ORF clone, Gallus gallus(chicken) -> XP_004937122.1 Gallus gallus thyroid hormone receptor interactor 12 (TRIP12), transcript variant X7, mRNA.

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CloneID	OGa12503
Clone ID Related Accession (Same CDS sequence)	XM_004937065.3 , XM_004937065.2
Accession Version	XM_004937065.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6183bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-03
Organism	Gallus gallus(chicken)
Product	E3 ubiquitin-protein ligase TRIP12 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_001471743.3) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 4, 2016 this sequence version replaced XM_004937065.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Gallus gallus Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCCAACC GGCCTAATAA CAATCCAGGG GGGTCACTGC GACGTTCACA GAGGAACACT 
GCTGGGGCCC AGCCACAAGA CGACGCAGCA GGAGGAAGGT CACATTTAGG GCAGGCAAAA 
CATAAGGCAC ATAGCCCTCC TGAGAGTAGA AAATCTATTT CAAAGACACC CAAAGTGCAG 
TCTAATACTA CTTCTGAGCA GTCCAAGGGA CACTTTTCTA AAAGAGGCTG TAGTTCATCT 
GCTGTTTTAA TACCACAAGA AGATTCAGAA AGAGTCAGTA CTTCAGAAAA GCAAAAAACG 
GGGCAAGTGC CTAAGAAAGA CAATTCTCGA GGAGTTAAAC GCAGTGCTAG TCCAGATTAC 
AGCAGGACCA ATTCTCCTAG CTCTGCTAAA AAACCTAAAG CACTTCAACA CGCCGAAACT 
TCCTCGGAAA GTAACAAGCC ACATACTAAA TCCAAGAGAA GACACTTAGA CCAAGAACAG 
CAGAAGTCTA CACAATTGCC ATCAACAAGC AAGGCTCACA CCAGAAAGGG TGGAGCTGCT 
GGTAGCTCCC GAAGTCAGAA AAGGAAAAGG ACAGAGAGTA CGTCTTGTAT AAAGAGTCGT 
TCAGCAGTTG AATCAACTGG AGCTGAAGAG AAGTCAGCAA AATCCTCCAA GCTGGCTTCA 
AAATCGGTGA CCTCAGCCAA AGCTGGGTGT AGCACCATCA CTGATTCTTC TTCTTCAGCT 
TCTACATCCT CTTCCTCTTC TGCTGTTGCC TCTGCTTCTT CCACTGTTCC TCAGGGTGCC 
AGAGTGAAAC AGGGTAAGGA CCAGAATAAG GCTAGACGTT CACGTTCTGC GTCCAGCCCT 
AGTCCAAGAA GGAGCAGCAG GGACAAAGAA CAGAGCAAAA CAGGTGGCTC TTCAAAGTTT 
GACTGGGCTG CTCGATTCAG TCCAAAAGTT AGTCTCCCTA AAACAAAACT GTCTCTACCA 
GGCTCTTCTA AGTCAGAGAC CTCAAAACCT GGACCTTCAG GACTACAGGC TAAGCTAGCA 
AGCCTAAGAA AATCCACAAA GAAGCGCAGT GAATCACCAC CTGCTGAGCT CCCCAGTTTA 
CGGCGGAGCA CACGGCAAAA GACCACGGGC TCCTGTGCGA GTACCAGTCG GCGGGGCTCT 
GGCCTGGGCA AAAGAGGAGC AGCTGAAGCT CGCCGACAGG AGAAGATGGC TGATCCTGAC 
AACAACCAGG ATGGAGTTAA CTCTTCAGCT GCACGTACAG ATGAGACTCC CCAGGGAGCT 
GCAGCTTCTA GCTCTGTTGC TGGGGCTGTA GGTATGACAA CATCTGGAGA AAGTGAGTCA 
GATGACTCTG AGATGGGAAG ACTACAAGCT CTGTTAGAGG CTAGGGGTCT TCCTCCTCAC 
CTGTTTGGCC CTCTTGGTCC TCGGATGTCG CAGCTCTTCC ACAGGACAAT TGGAAGTGGA 
GCTAGTTCTA AAGCCCAGCA GCTTTTACAA GGTCTCCAAG CCACTGATGA AAGTCAGCAA 
CTACAGGCAG TTATTGAGAT GTGCCAGCTG TTGGTCATGG GAAATGAAGA AACACTGGGA 
GGATTTCCAG TCAAGAGTGT TGTGCCAGCT TTGATAACAT TGCTGCAGAT GGAGCATAAC 
TTTGACATCA TGAACCATGC TTGTCGAGCC TTAACATACA TGATGGAAGC ACTTCCCAGA 
TCATCTGCTG TAGTGGTAGA TGCAATTCCT GTCTTCTTGG AAAAGCTGCA AGTTATTCAG 
TGCATTGATG TGGCAGAGCA GGCACTGACA GCCCTAGAGA TGCTATCACG CAGGCATAGT 
AAAGCCATTC TGCAGGCAGG TGGGTTGGCA GACTGCTTGC TGTATCTGGA ATTCTTCAGT 
ATAAATGCGC AGAGGAATGC ACTAGCTATT GCTGCCAATT GCTGCCAGAG TATAACACCT 
GATGAGTTTC ACTTTGTGGC AGACTCTTTG CCACTGCTTA CACAAAGGTT AACCCATCAG 
GACAAGAAGT CTGTTGAAAG CACTTGTCTC TGTTTTGCAC GGCTGGTAGA CAATTTCCAG 
CATGAGGAGA ACTTGCTGCA GCAGGTTGCT TCCAAGGACT TGTTAACAAA CATCCAGCAA 
CTCTTGGTAG TGACACCTCC TATCCTGAGC TCAGGAATGT TCATCATGGT GGTGCGCATG 
TTTTCCTTAA TGTGTTCCAA TTGTCCAACG CTTGCAGTCC AACTTATGAA GCAAAACATT 
GCAGAAACAC TTCACTTTCT CCTTTGTGGA GCCTCAAATG GGAGTTGTCA AGAACAAATT 
GACCTTGTTC CACGAAGTCC TCAAGAACTC TATGAGCTTA CTTCTCTTAT CTGTGAACTG 
ATGCCTTGTC TGCCAAAAGA GGGGATATTT GCTGTTGATA CTATGCTAAA GAAAGGAAAT 
GCACAAAATA CAGATGGTGC GATATGGCAA TGGCGAGATG ACAGGGGTCT CTGGCATCCC 
TATAACAGGA TTGATAGTCG AATAATAGAG GCAGCTCATC AGGTTGGTGA GGATGAGATA 
AGCCTGTCGA CACTTGGACG TGTCTATACT ATTGATTTTA ATTCTATGCA GCAAATAAAT 
GAGGATACTG GAACAGCACG TGCCATTCAG CGGAAACCAA ACCCTTTAGC CAATACAAAC 
ACTAGTGGAC ATTCAGAATT GAAGAAGGAT GATGCTCGAG CACAGCTAAT GAAAGAAGAC 
CCAGAATTGG CAAAATCCTT TATCAAAACA CTGTTTGGTG TTCTTTATGA AGTGTATAGT 
TCTTCAGCTG GACCTGCTGT TAGACACAAG TGCCTTAGAG CAATACTTAG AATAATTTAT 
TTTGCTGATG CTGAACTCCT GAAGGATGTG CTGAAAAACC ATGCTGTGTC AAGTCACATT 
GCCTCCATGC TGTCAAGTCA AGACCTTAAG ATAGTAGTTG GAGCCCTGCA GATGGCAGAA 
ATCTTAATGC AGAAGCTACC TGACATTTTT AGTGTTTACT TCAGAAGAGA AGGAGTGATG 
CATCAAGTGA AAAACTTAGC GGAGTCTGAG GCTTTGCTAA CGAGCCCACC AAAAGTATGC 
ACTAATGGAT CAGGAACACT GGGTACCACT ACAACTATAA GTACTGGAAC AGCCACTGCT 
GCCACTAATG CAGCTGCAGA TTTGGGCTCT CCCAGCTTAC AACACAGCCG GGAGGATTCT 
TTAGATCTGA GCCCACAGGG ACGACTGAGT GATGTTCTGA AGAGAAAAAG ACTGCCAAAA 
CGAGGGCCAA GGAGACCAAA ATATTCTCCC CCAAGAGATG ATGACAAAGT AGACAATCAA 
GCAAAAAGCC CCACGACTAC TCAGTCTCCT AAATCTTCTT TCTTAGCAAG TTTAAATCCC 
AAAACATGGG GAAGATTAAG TACACAGTCT AACAGTAATA ACATTGAACC AGCAAGAACA 
GCAGGTGTAA GTGGTCTTGC AAGAGCTGCT TCTAAGGATA CAATTTCCAA TAACAGAGAA 
AAAATTAAGG GTTGGATTAA GGAGCAAGCC CATAAATTTG TAGACCGTTA TTTTAGTTCG 
GAAAACATGG ATGGAAGCAA TCCTGCACTA AATGTATTAC AGAGACTTTG CACTGCAACT 
GAACAACTTA ACCTCCAGGT GGATGGTGGA ACAGAGTGCC TTGTAGAAAT CCGTAGCATT 
GTCTCGGAGT CTGACGTCTC CTCATTTGAA ATCCAGCATA GTGGATTTGT GAAACAACTG 
CTGCTTTATT TGACATCTAA AAGTGAGAAA GATGCTGTAA GCAGGGATAT CAGATTGAAA 
AGATTTCTTC ATGTATTTTT CTCCTCTCCA CTTCCTGGAG AAGAACCCCT TGGAAGATTA 
GAGCCATTAG AAAATGCACC TTTGTTGGCA TTAGTCCATA AAATGAACAA TTGCCTCAGT 
CAGATGGAGC AGTTTCCAGT GAAAGTGCAT GACTTCCCCA GTGGAAATGG AACAGGGAGC 
AGAGGATCCC AAGCTTTAAA ATTCTTCAAT ACGCATCAGT TAAAATGCCA GCTGCAAAGA 
CATCCAGACT GTGCTAATGT GAAACAGTGG AAGGGTGGAC CTGTGAAGAT TGATCCTCTG 
GCTTTGGTTC AAGCCATTGA AAGATATCTT GTTGTTAGAG GTTATGGAAG AGTTAGAGAA 
GATGATGAGG ATAGTGATGA TGATGGGTCA GATGAAGAAA TAGATGAATC CTTGGCTGCT 
CAATTCTTAA ATTCAGGGAA TGTGAGACAT AGATTGCAGT TTTACATTGG GGATCACTTG 
CTACCATATA ATATGACTGT ATATCAAGCA GTTAAGCTGT ACAGTTTGCA AGCTGAGGAG 
GAGAGGGAAT CGACTGATGA TGAAAGCAAT CCATTAGGAA GAGCTGGGAT TTGGACAAAA 
ACACATACCA TTTGGTACAA ACCTGTACGG GAGGATGAAG ATGGCAACAA AGACTGTGTT 
GGTGGTAAAA GAGGAAGAGC TCAAACTGCT CCCACAAAAA CCTCACCTAG GAATTCTAAA 
AAGCATGATG AGTTGTGGCA CGATGGTGTA TGCCCTTCAG TATTGAATCC TTTAGAAGTT 
TACCTCATAT CTACTCCTCC TGAAAACATA ACCTTTGAAG ATCCTTCATT AGATGTTATT 
CTTCTTTTGA GAGTTTTACA TGCTATCAGT CGATACTGGT ATTATTTGTA TGATAATGCA 
ATATGCAAGG AGATTATTCC AACTTCGGAA TTTATCAATA GTAAACTGAC AGCAAAAGCA 
AACAGACAGC TTCAAGATCC ACTGGTAATT ATGACAGGAA ACATACCGAC TTGGCTGACA 
GAGCTTGGAA AAACATGCCC ATTTTTCTTT CCATTTGATA CCCGGCAAAT GCTGTTTTAT 
GTAACTGCTT TTGATCGTGA TCGAGCCATG CAAAGACTAC TGGATACTAA TCCAGAAATC 
AATCAATCAG ATTCTCAGGA TAGCAGAGTG GCACCACGAC TGGACAGGAA AAAACGCACT 
GTGAACAGAG ATGAGCTGTT GAAACAGGCA GAATCTGTGA TGCAGGATCT AGGCAGTTCA 
AGAGCCATGT TGGAAATCCA GTATGAGAAT GAAGTTGGCA CAGGCCTGGG CCCTACACTA 
GAGTTCTATG CACTTGTATC TCAGGAACTA CAGAGAGCAG ACTTGGGCCT TTGGAGGGGA 
GAAGAAGTAA CTTTAGCCAA TCCAAAAGGA AGCCAAGAAG GTACCAAGTA TATTCATAAT 
CTCCAAGGCC TTTTTGCACT TCCTTTTGGT AGAACAGCCA AGCCTGCTCA CATTGCAAAA 
GTTAAAATGA AGTTCCGCTT TCTGGGAAAA CTAATGGCTA AGGCAATCAT GGATTTTAGA 
CTGGTGGATC TTCCTCTTGG ACTTCCTTTT TATAAATGGA TGTTACGCCA GGAAACTTCT 
TTGACGTCAC ATGACTTGTT CAGTATTGAT CCAGTAGTAG CCAAATCAAT ATATCACCTT 
GAAGATATTG TAAGACAAAA GAAAAGACTT GAGCAGGACA AAACACAGAC CAAAGAAAGT 
CTACAGTATG CATTGGAAAC CCTGACTATG AATGGCTGCT CAGTGGAGGA TCTAGGGCTG 
GATTTCACAC TTCCTGGATT TCCTAACATC GAGCTGAAAA AAGGGGGAAA AGATACACCA 
GTCACCATCC ACAACTTGGA GGAGTACCTC AGATTGGTTA TATTCTGGGC ACTTAACGAA 
GGTGTTGCCA GACAGTTTGA TTCTTTCAGA GATGGATTTG AATCAGTCTT CCCCCTCAGT 
CATCTTCAAT ACTTTTATCC TGAGGAGTTG GAGCAACTCT TATGTGGCAG CAAAACAGAC 
ACATGGGATG CCAAGACCTT AATGGAATGT TGCAGGCCAG ATCACGGTTA TACACATGAC 
AGTCGAGCAG TGAAGTATCT CTTTGAAATT CTCAGTAGTT TTGATAGTGA GCAGCAGAGA 
CTGTTTCTTC AGTTTGTGAC CGGTAGTCCC AGGCTGCCAG TAGGAGGCTT TCGAAGTTTG 
AATCCTCCAT TGACAATTGT ACGGAAGACA TTTGAGTCTA CAGAGAATCC AGATGACTTC 
TTGCCCTCAG TAATGACTTG TGTGAACTAT CTCAAATTGC CGGACTATTC AACTATTGAG 
ATAATGCGTG AAAAACTCTT GATAGCTGCA AGAGAAGGGC AGCAGTCATT CCATCTTTCC 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_004937122.1
CDS	150..6332
Translation	MSNRPNNNPGGSLRRSQRNTAGAQPQDDAAGGRSHLGQAKHKAHSPPESRKSISKTPKVQSNTTSEQSKGHFSKRGCSSSAVLIPQEDSERVSTSEKQKTGQVPKKDNSRGVKRSASPDYSRTNSPSSAKKPKALQHAETSSESNKPHTKSKRRHLDQEQQKSTQLPSTSKAHTRKGGAAGSSRSQKRKRTESTSCIKSRSAVESTGAEEKSAKSSKLASKSVTSAKAGCSTITDSSSSASTSSSSSAVASASSTVPQGARVKQGKDQNKARRSRSASSPSPRRSSRDKEQSKTGGSSKFDWAARFSPKVSLPKTKLSLPGSSKSETSKPGPSGLQAKLASLRKSTKKRSESPPAELPSLRRSTRQKTTGSCASTSRRGSGLGKRGAAEARRQEKMADPDNNQDGVNSSAARTDETPQGAAASSSVAGAVGMTTSGESESDDSEMGRLQALLEARGLPPHLFGPLGPRMSQLFHRTIGSGASSKAQQLLQGLQATDESQQLQAVIEMCQLLVMGNEETLGGFPVKSVVPALITLLQMEHNFDIMNHACRALTYMMEALPRSSAVVVDAIPVFLEKLQVIQCIDVAEQALTALEMLSRRHSKAILQAGGLADCLLYLEFFSINAQRNALAIAANCCQSITPDEFHFVADSLPLLTQRLTHQDKKSVESTCLCFARLVDNFQHEENLLQQVASKDLLTNIQQLLVVTPPILSSGMFIMVVRMFSLMCSNCPTLAVQLMKQNIAETLHFLLCGASNGSCQEQIDLVPRSPQELYELTSLICELMPCLPKEGIFAVDTMLKKGNAQNTDGAIWQWRDDRGLWHPYNRIDSRIIEAAHQVGEDEISLSTLGRVYTIDFNSMQQINEDTGTARAIQRKPNPLANTNTSGHSELKKDDARAQLMKEDPELAKSFIKTLFGVLYEVYSSSAGPAVRHKCLRAILRIIYFADAELLKDVLKNHAVSSHIASMLSSQDLKIVVGALQMAEILMQKLPDIFSVYFRREGVMHQVKNLAESEALLTSPPKVCTNGSGTLGTTTTISTGTATAATNAAADLGSPSLQHSREDSLDLSPQGRLSDVLKRKRLPKRGPRRPKYSPPRDDDKVDNQAKSPTTTQSPKSSFLASLNPKTWGRLSTQSNSNNIEPARTAGVSGLARAASKDTISNNREKIKGWIKEQAHKFVDRYFSSENMDGSNPALNVLQRLCTATEQLNLQVDGGTECLVEIRSIVSESDVSSFEIQHSGFVKQLLLYLTSKSEKDAVSRDIRLKRFLHVFFSSPLPGEEPLGRLEPLENAPLLALVHKMNNCLSQMEQFPVKVHDFPSGNGTGSRGSQALKFFNTHQLKCQLQRHPDCANVKQWKGGPVKIDPLALVQAIERYLVVRGYGRVREDDEDSDDDGSDEEIDESLAAQFLNSGNVRHRLQFYIGDHLLPYNMTVYQAVKLYSLQAEEERESTDDESNPLGRAGIWTKTHTIWYKPVREDEDGNKDCVGGKRGRAQTAPTKTSPRNSKKHDELWHDGVCPSVLNPLEVYLISTPPENITFEDPSLDVILLLRVLHAISRYWYYLYDNAICKEIIPTSEFINSKLTAKANRQLQDPLVIMTGNIPTWLTELGKTCPFFFPFDTRQMLFYVTAFDRDRAMQRLLDTNPEINQSDSQDSRVAPRLDRKKRTVNRDELLKQAESVMQDLGSSRAMLEIQYENEVGTGLGPTLEFYALVSQELQRADLGLWRGEEVTLANPKGSQEGTKYIHNLQGLFALPFGRTAKPAHIAKVKMKFRFLGKLMAKAIMDFRLVDLPLGLPFYKWMLRQETSLTSHDLFSIDPVVAKSIYHLEDIVRQKKRLEQDKTQTKESLQYALETLTMNGCSVEDLGLDFTLPGFPNIELKKGGKDTPVTIHNLEEYLRLVIFWALNEGVARQFDSFRDGFESVFPLSHLQYFYPEELEQLLCGSKTDTWDAKTLMECCRPDHGYTHDSRAVKYLFEILSSFDSEQQRLFLQFVTGSPRLPVGGFRSLNPPLTIVRKTFESTENPDDFLPSVMTCVNYLKLPDYSTIEIMREKLLIAAREGQQSFHLS

Target ORF information:

RefSeq Version	XM_004937065.2
Organism	Gallus gallus(chicken)
Definition	Gallus gallus thyroid hormone receptor interactor 12 (TRIP12), transcript variant X7, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_004937065.2

ORF Insert Sequence:

ATGTCCAACC GGCCTAATAA CAATCCAGGG GGGTCACTGC GACGTTCACA GAGGAACACT 
GCTGGGGCCC AGCCACAAGA CGACGCAGCA GGAGGAAGGT CACATTTAGG GCAGGCAAAA 
CATAAGGCAC ATAGCCCTCC TGAGAGTAGA AAATCTATTT CAAAGACACC CAAAGTGCAG 
TCTAATACTA CTTCTGAGCA GTCCAAGGGA CACTTTTCTA AAAGAGGCTG TAGTTCATCT 
GCTGTTTTAA TACCACAAGA AGATTCAGAA AGAGTCAGTA CTTCAGAAAA GCAAAAAACG 
GGGCAAGTGC CTAAGAAAGA CAATTCTCGA GGAGTTAAAC GCAGTGCTAG TCCAGATTAC 
AGCAGGACCA ATTCTCCTAG CTCTGCTAAA AAACCTAAAG CACTTCAACA CGCCGAAACT 
TCCTCGGAAA GTAACAAGCC ACATACTAAA TCCAAGAGAA GACACTTAGA CCAAGAACAG 
CAGAAGTCTA CACAATTGCC ATCAACAAGC AAGGCTCACA CCAGAAAGGG TGGAGCTGCT 
GGTAGCTCCC GAAGTCAGAA AAGGAAAAGG ACAGAGAGTA CGTCTTGTAT AAAGAGTCGT 
TCAGCAGTTG AATCAACTGG AGCTGAAGAG AAGTCAGCAA AATCCTCCAA GCTGGCTTCA 
AAATCGGTGA CCTCAGCCAA AGCTGGGTGT AGCACCATCA CTGATTCTTC TTCTTCAGCT 
TCTACATCCT CTTCCTCTTC TGCTGTTGCC TCTGCTTCTT CCACTGTTCC TCAGGGTGCC 
AGAGTGAAAC AGGGTAAGGA CCAGAATAAG GCTAGACGTT CACGTTCTGC GTCCAGCCCT 
AGTCCAAGAA GGAGCAGCAG GGACAAAGAA CAGAGCAAAA CAGGTGGCTC TTCAAAGTTT 
GACTGGGCTG CTCGATTCAG TCCAAAAGTT AGTCTCCCTA AAACAAAACT GTCTCTACCA 
GGCTCTTCTA AGTCAGAGAC CTCAAAACCT GGACCTTCAG GACTACAGGC TAAGCTAGCA 
AGCCTAAGAA AATCCACAAA GAAGCGCAGT GAATCACCAC CTGCTGAGCT CCCCAGTTTA 
CGGCGGAGCA CACGGCAAAA GACCACGGGC TCCTGTGCGA GTACCAGTCG GCGGGGCTCT 
GGCCTGGGCA AAAGAGGAGC AGCTGAAGCT CGCCGACAGG AGAAGATGGC TGATCCTGAC 
AACAACCAGG ATGGAGTTAA CTCTTCAGCT GCACGTACAG ATGAGACTCC CCAGGGAGCT 
GCAGCTTCTA GCTCTGTTGC TGGGGCTGTA GGTATGACAA CATCTGGAGA AAGTGAGTCA 
GATGACTCTG AGATGGGAAG ACTACAAGCT CTGTTAGAGG CTAGGGGTCT TCCTCCTCAC 
CTGTTTGGCC CTCTTGGTCC TCGGATGTCG CAGCTCTTCC ACAGGACAAT TGGAAGTGGA 
GCTAGTTCTA AAGCCCAGCA GCTTTTACAA GGTCTCCAAG CCACTGATGA AAGTCAGCAA 
CTACAGGCAG TTATTGAGAT GTGCCAGCTG TTGGTCATGG GAAATGAAGA AACACTGGGA 
GGATTTCCAG TCAAGAGTGT TGTGCCAGCT TTGATAACAT TGCTGCAGAT GGAGCATAAC 
TTTGACATCA TGAACCATGC TTGTCGAGCC TTAACATACA TGATGGAAGC ACTTCCCAGA 
TCATCTGCTG TAGTGGTAGA TGCAATTCCT GTCTTCTTGG AAAAGCTGCA AGTTATTCAG 
TGCATTGATG TGGCAGAGCA GGCACTGACA GCCCTAGAGA TGCTATCACG CAGGCATAGT 
AAAGCCATTC TGCAGGCAGG TGGGTTGGCA GACTGCTTGC TGTATCTGGA ATTCTTCAGT 
ATAAATGCGC AGAGGAATGC ACTAGCTATT GCTGCCAATT GCTGCCAGAG TATAACACCT 
GATGAGTTTC ACTTTGTGGC AGACTCTTTG CCACTGCTTA CACAAAGGTT AACCCATCAG 
GACAAGAAGT CTGTTGAAAG CACTTGTCTC TGTTTTGCAC GGCTGGTAGA CAATTTCCAG 
CATGAGGAGA ACTTGCTGCA GCAGGTTGCT TCCAAGGACT TGTTAACAAA CATCCAGCAA 
CTCTTGGTAG TGACACCTCC TATCCTGAGC TCAGGAATGT TCATCATGGT GGTGCGCATG 
TTTTCCTTAA TGTGTTCCAA TTGTCCAACG CTTGCAGTCC AACTTATGAA GCAAAACATT 
GCAGAAACAC TTCACTTTCT CCTTTGTGGA GCCTCAAATG GGAGTTGTCA AGAACAAATT 
GACCTTGTTC CACGAAGTCC TCAAGAACTC TATGAGCTTA CTTCTCTTAT CTGTGAACTG 
ATGCCTTGTC TGCCAAAAGA GGGGATATTT GCTGTTGATA CTATGCTAAA GAAAGGAAAT 
GCACAAAATA CAGATGGTGC GATATGGCAA TGGCGAGATG ACAGGGGTCT CTGGCATCCC 
TATAACAGGA TTGATAGTCG AATAATAGAG GCAGCTCATC AGGTTGGTGA GGATGAGATA 
AGCCTGTCGA CACTTGGACG TGTCTATACT ATTGATTTTA ATTCTATGCA GCAAATAAAT 
GAGGATACTG GAACAGCACG TGCCATTCAG CGGAAACCAA ACCCTTTAGC CAATACAAAC 
ACTAGTGGAC ATTCAGAATT GAAGAAGGAT GATGCTCGAG CACAGCTAAT GAAAGAAGAC 
CCAGAATTGG CAAAATCCTT TATCAAAACA CTGTTTGGTG TTCTTTATGA AGTGTATAGT 
TCTTCAGCTG GACCTGCTGT TAGACACAAG TGCCTTAGAG CAATACTTAG AATAATTTAT 
TTTGCTGATG CTGAACTCCT GAAGGATGTG CTGAAAAACC ATGCTGTGTC AAGTCACATT 
GCCTCCATGC TGTCAAGTCA AGACCTTAAG ATAGTAGTTG GAGCCCTGCA GATGGCAGAA 
ATCTTAATGC AGAAGCTACC TGACATTTTT AGTGTTTACT TCAGAAGAGA AGGAGTGATG 
CATCAAGTGA AAAACTTAGC GGAGTCTGAG GCTTTGCTAA CGAGCCCACC AAAAGTATGC 
ACTAATGGAT CAGGAACACT GGGTACCACT ACAACTATAA GTACTGGAAC AGCCACTGCT 
GCCACTAATG CAGCTGCAGA TTTGGGCTCT CCCAGCTTAC AACACAGCCG GGAGGATTCT 
TTAGATCTGA GCCCACAGGG ACGACTGAGT GATGTTCTGA AGAGAAAAAG ACTGCCAAAA 
CGAGGGCCAA GGAGACCAAA ATATTCTCCC CCAAGAGATG ATGACAAAGT AGACAATCAA 
GCAAAAAGCC CCACGACTAC TCAGTCTCCT AAATCTTCTT TCTTAGCAAG TTTAAATCCC 
AAAACATGGG GAAGATTAAG TACACAGTCT AACAGTAATA ACATTGAACC AGCAAGAACA 
GCAGGTGTAA GTGGTCTTGC AAGAGCTGCT TCTAAGGATA CAATTTCCAA TAACAGAGAA 
AAAATTAAGG GTTGGATTAA GGAGCAAGCC CATAAATTTG TAGACCGTTA TTTTAGTTCG 
GAAAACATGG ATGGAAGCAA TCCTGCACTA AATGTATTAC AGAGACTTTG CACTGCAACT 
GAACAACTTA ACCTCCAGGT GGATGGTGGA ACAGAGTGCC TTGTAGAAAT CCGTAGCATT 
GTCTCGGAGT CTGACGTCTC CTCATTTGAA ATCCAGCATA GTGGATTTGT GAAACAACTG 
CTGCTTTATT TGACATCTAA AAGTGAGAAA GATGCTGTAA GCAGGGATAT CAGATTGAAA 
AGATTTCTTC ATGTATTTTT CTCCTCTCCA CTTCCTGGAG AAGAACCCCT TGGAAGATTA 
GAGCCATTAG AAAATGCACC TTTGTTGGCA TTAGTCCATA AAATGAACAA TTGCCTCAGT 
CAGATGGAGC AGTTTCCAGT GAAAGTGCAT GACTTCCCCA GTGGAAATGG AACAGGGAGC 
AGAGGATCCC AAGCTTTAAA ATTCTTCAAT ACGCATCAGT TAAAATGCCA GCTGCAAAGA 
CATCCAGACT GTGCTAATGT GAAACAGTGG AAGGGTGGAC CTGTGAAGAT TGATCCTCTG 
GCTTTGGTTC AAGCCATTGA AAGATATCTT GTTGTTAGAG GTTATGGAAG AGTTAGAGAA 
GATGATGAGG ATAGTGATGA TGATGGGTCA GATGAAGAAA TAGATGAATC CTTGGCTGCT 
CAATTCTTAA ATTCAGGGAA TGTGAGACAT AGATTGCAGT TTTACATTGG GGATCACTTG 
CTACCATATA ATATGACTGT ATATCAAGCA GTTAAGCTGT ACAGTTTGCA AGCTGAGGAG 
GAGAGGGAAT CGACTGATGA TGAAAGCAAT CCATTAGGAA GAGCTGGGAT TTGGACAAAA 
ACACATACCA TTTGGTACAA ACCTGTACGG GAGGATGAAG ATGGCAACAA AGACTGTGTT 
GGTGGTAAAA GAGGAAGAGC TCAAACTGCT CCCACAAAAA CCTCACCTAG GAATTCTAAA 
AAGCATGATG AGTTGTGGCA CGATGGTGTA TGCCCTTCAG TATTGAATCC TTTAGAAGTT 
TACCTCATAT CTACTCCTCC TGAAAACATA ACCTTTGAAG ATCCTTCATT AGATGTTATT 
CTTCTTTTGA GAGTTTTACA TGCTATCAGT CGATACTGGT ATTATTTGTA TGATAATGCA 
ATATGCAAGG AGATTATTCC AACTTCGGAA TTTATCAATA GTAAACTGAC AGCAAAAGCA 
AACAGACAGC TTCAAGATCC ACTGGTAATT ATGACAGGAA ACATACCGAC TTGGCTGACA 
GAGCTTGGAA AAACATGCCC ATTTTTCTTT CCATTTGATA CCCGGCAAAT GCTGTTTTAT 
GTAACTGCTT TTGATCGTGA TCGAGCCATG CAAAGACTAC TGGATACTAA TCCAGAAATC 
AATCAATCAG ATTCTCAGGA TAGCAGAGTG GCACCACGAC TGGACAGGAA AAAACGCACT 
GTGAACAGAG ATGAGCTGTT GAAACAGGCA GAATCTGTGA TGCAGGATCT AGGCAGTTCA 
AGAGCCATGT TGGAAATCCA GTATGAGAAT GAAGTTGGCA CAGGCCTGGG CCCTACACTA 
GAGTTCTATG CACTTGTATC TCAGGAACTA CAGAGAGCAG ACTTGGGCCT TTGGAGGGGA 
GAAGAAGTAA CTTTAGCCAA TCCAAAAGGA AGCCAAGAAG GTACCAAGTA TATTCATAAT 
CTCCAAGGCC TTTTTGCACT TCCTTTTGGT AGAACAGCCA AGCCTGCTCA CATTGCAAAA 
GTTAAAATGA AGTTCCGCTT TCTGGGAAAA CTAATGGCTA AGGCAATCAT GGATTTTAGA 
CTGGTGGATC TTCCTCTTGG ACTTCCTTTT TATAAATGGA TGTTACGCCA GGAAACTTCT 
TTGACGTCAC ATGACTTGTT CAGTATTGAT CCAGTAGTAG CCAAATCAAT ATATCACCTT 
GAAGATATTG TAAGACAAAA GAAAAGACTT GAGCAGGACA AAACACAGAC CAAAGAAAGT 
CTACAGTATG CATTGGAAAC CCTGACTATG AATGGCTGCT CAGTGGAGGA TCTAGGGCTG 
GATTTCACAC TTCCTGGATT TCCTAACATC GAGCTGAAAA AAGGGGGAAA AGATACACCA 
GTCACCATCC ACAACTTGGA GGAGTACCTC AGATTGGTTA TATTCTGGGC ACTTAACGAA 
GGTGTTGCCA GACAGTTTGA TTCTTTCAGA GATGGATTTG AATCAGTCTT CCCCCTCAGT 
CATCTTCAAT ACTTTTATCC TGAGGAGTTG GAGCAACTCT TATGTGGCAG CAAAACAGAC 
ACATGGGATG CCAAGACCTT AATGGAATGT TGCAGGCCAG ATCACGGTTA TACACATGAC 
AGTCGAGCAG TGAAGTATCT CTTTGAAATT CTCAGTAGTT TTGATAGTGA GCAGCAGAGA 
CTGTTTCTTC AGTTTGTGAC CGGTAGTCCC AGGCTGCCAG TAGGAGGCTT TCGAAGTTTG 
AATCCTCCAT TGACAATTGT ACGGAAGACA TTTGAGTCTA CAGAGAATCC AGATGACTTC 
TTGCCCTCAG TAATGACTTG TGTGAACTAT CTCAAATTGC CGGACTATTC AACTATTGAG 
ATAATGCGTG AAAAACTCTT GATAGCTGCA AGAGAAGGGC AGCAGTCATT CCATCTTTCC 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TRIP12 ( XM_015276971.1 ) cDNA ORF clone, Gallus gallus(chicken) -> XP_015132457.1 Gallus gallus thyroid hormone receptor interactor 12 (TRIP12), transcript variant X10, mRNA.

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CloneID	OGa12504
Clone ID Related Accession (Same CDS sequence)	XM_015276971.1 , XM_015276971.2
Accession Version	XM_015276971.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6072bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-03
Organism	Gallus gallus(chicken)
Product	E3 ubiquitin-protein ligase TRIP12 isoform X5
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_001471743.3) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Gallus gallus Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCCAACC GGCCTAATAA CAATCCAGGG GGGTCACTGC GACGTTCACA GAGGAACACT 
GCTGGGGCCC AGCCACAAGA CGACGCAGCA GGAGGAAGAG GCTGTAGTTC ATCTGCTGTT 
TTAATACCAC AAGAAGATTC AGAAAGAGTC AGTACTTCAG AAAAGCAAAA AACGGGGCAA 
GTGCCTAAGA AAGACAATTC TCGAGGAGTT AAACGCAGTG CTAGTCCAGA TTACAGCAGG 
ACCAATTCTC CTAGCTCTGC TAAAAAACCT AAAGCACTTC AACACGCCGA AACTTCCTCG 
GAAAGTAACA AGCCACATAC TAAATCCAAG AGAAGACACT TAGACCAAGA ACAGCAGAAG 
TCTACACAAT TGCCATCAAC AAGCAAGGCT CACACCAGAA AGGGTGGAGC TGCTGGTAGC 
TCCCGAAGTC AGAAAAGGAA AAGGACAGAG AGTACGTCTT GTATAAAGAG TCGTTCAGCA 
GTTGAATCAA CTGGAGCTGA AGAGAAGTCA GCAAAATCCT CCAAGCTGGC TTCAAAATCG 
GTGACCTCAG CCAAAGCTGG GTGTAGCACC ATCACTGATT CTTCTTCTTC AGCTTCTACA 
TCCTCTTCCT CTTCTGCTGT TGCCTCTGCT TCTTCCACTG TTCCTCAGGG TGCCAGAGTG 
AAACAGGGTA AGGACCAGAA TAAGGCTAGA CGTTCACGTT CTGCGTCCAG CCCTAGTCCA 
AGAAGGAGCA GCAGGGACAA AGAACAGAGC AAAACAGGTG GCTCTTCAAA GTTTGACTGG 
GCTGCTCGAT TCAGTCCAAA AGTTAGTCTC CCTAAAACAA AACTGTCTCT ACCAGGCTCT 
TCTAAGTCAG AGACCTCAAA ACCTGGACCT TCAGGACTAC AGGCTAAGCT AGCAAGCCTA 
AGAAAATCCA CAAAGAAGCG CAGTGAATCA CCACCTGCTG AGCTCCCCAG TTTACGGCGG 
AGCACACGGC AAAAGACCAC GGGCTCCTGT GCGAGTACCA GTCGGCGGGG CTCTGGCCTG 
GGCAAAAGAG GAGCAGCTGA AGCTCGCCGA CAGGAGAAGA TGGCTGATCC TGACAACAAC 
CAGGATGGAG TTAACTCTTC AGCTGCACGT ACAGATGAGA CTCCCCAGGG AGCTGCAGCT 
TCTAGCTCTG TTGCTGGGGC TGTAGGTATG ACAACATCTG GAGAAAGTGA GTCAGATGAC 
TCTGAGATGG GAAGACTACA AGCTCTGTTA GAGGCTAGGG GTCTTCCTCC TCACCTGTTT 
GGCCCTCTTG GTCCTCGGAT GTCGCAGCTC TTCCACAGGA CAATTGGAAG TGGAGCTAGT 
TCTAAAGCCC AGCAGCTTTT ACAAGGTCTC CAAGCCACTG ATGAAAGTCA GCAACTACAG 
GCAGTTATTG AGATGTGCCA GCTGTTGGTC ATGGGAAATG AAGAAACACT GGGAGGATTT 
CCAGTCAAGA GTGTTGTGCC AGCTTTGATA ACATTGCTGC AGATGGAGCA TAACTTTGAC 
ATCATGAACC ATGCTTGTCG AGCCTTAACA TACATGATGG AAGCACTTCC CAGATCATCT 
GCTGTAGTGG TAGATGCAAT TCCTGTCTTC TTGGAAAAGC TGCAAGTTAT TCAGTGCATT 
GATGTGGCAG AGCAGGCACT GACAGCCCTA GAGATGCTAT CACGCAGGCA TAGTAAAGCC 
ATTCTGCAGG CAGGTGGGTT GGCAGACTGC TTGCTGTATC TGGAATTCTT CAGTATAAAT 
GCGCAGAGGA ATGCACTAGC TATTGCTGCC AATTGCTGCC AGAGTATAAC ACCTGATGAG 
TTTCACTTTG TGGCAGACTC TTTGCCACTG CTTACACAAA GGTTAACCCA TCAGGACAAG 
AAGTCTGTTG AAAGCACTTG TCTCTGTTTT GCACGGCTGG TAGACAATTT CCAGCATGAG 
GAGAACTTGC TGCAGCAGGT TGCTTCCAAG GACTTGTTAA CAAACATCCA GCAACTCTTG 
GTAGTGACAC CTCCTATCCT GAGCTCAGGA ATGTTCATCA TGGTGGTGCG CATGTTTTCC 
TTAATGTGTT CCAATTGTCC AACGCTTGCA GTCCAACTTA TGAAGCAAAA CATTGCAGAA 
ACACTTCACT TTCTCCTTTG TGGAGCCTCA AATGGGAGTT GTCAAGAACA AATTGACCTT 
GTTCCACGAA GTCCTCAAGA ACTCTATGAG CTTACTTCTC TTATCTGTGA ACTGATGCCT 
TGTCTGCCAA AAGAGGGGAT ATTTGCTGTT GATACTATGC TAAAGAAAGG AAATGCACAA 
AATACAGATG GTGCGATATG GCAATGGCGA GATGACAGGG GTCTCTGGCA TCCCTATAAC 
AGGATTGATA GTCGAATAAT AGAGGCAGCT CATCAGGTTG GTGAGGATGA GATAAGCCTG 
TCGACACTTG GACGTGTCTA TACTATTGAT TTTAATTCTA TGCAGCAAAT AAATGAGGAT 
ACTGGAACAG CACGTGCCAT TCAGCGGAAA CCAAACCCTT TAGCCAATAC AAACACTAGT 
GGACATTCAG AATTGAAGAA GGATGATGCT CGAGCACAGC TAATGAAAGA AGACCCAGAA 
TTGGCAAAAT CCTTTATCAA AACACTGTTT GGTGTTCTTT ATGAAGTGTA TAGTTCTTCA 
GCTGGACCTG CTGTTAGACA CAAGTGCCTT AGAGCAATAC TTAGAATAAT TTATTTTGCT 
GATGCTGAAC TCCTGAAGGA TGTGCTGAAA AACCATGCTG TGTCAAGTCA CATTGCCTCC 
ATGCTGTCAA GTCAAGACCT TAAGATAGTA GTTGGAGCCC TGCAGATGGC AGAAATCTTA 
ATGCAGAAGC TACCTGACAT TTTTAGTGTT TACTTCAGAA GAGAAGGAGT GATGCATCAA 
GTGAAAAACT TAGCGGAGTC TGAGGCTTTG CTAACGAGCC CACCAAAAGT ATGCACTAAT 
GGATCAGGAA CACTGGGTAC CACTACAACT ATAAGTACTG GAACAGCCAC TGCTGCCACT 
AATGCAGCTG CAGATTTGGG CTCTCCCAGC TTACAACACA GCCGGGAGGA TTCTTTAGAT 
CTGAGCCCAC AGGGACGACT GAGTGATGTT CTGAAGAGAA AAAGACTGCC AAAACGAGGG 
CCAAGGAGAC CAAAATATTC TCCCCCAAGA GATGATGACA AAGTAGACAA TCAAGCAAAA 
AGCCCCACGA CTACTCAGTC TCCTAAATCT TCTTTCTTAG CAAGTTTAAA TCCCAAAACA 
TGGGGAAGAT TAAGTACACA GTCTAACAGT AATAACATTG AACCAGCAAG AACAGCAGGT 
GTAAGTGGTC TTGCAAGAGC TGCTTCTAAG GATACAATTT CCAATAACAG AGAAAAAATT 
AAGGGTTGGA TTAAGGAGCA AGCCCATAAA TTTGTAGACC GTTATTTTAG TTCGGAAAAC 
ATGGATGGAA GCAATCCTGC ACTAAATGTA TTACAGAGAC TTTGCACTGC AACTGAACAA 
CTTAACCTCC AGGTGGATGG TGGAACAGAG TGCCTTGTAG AAATCCGTAG CATTGTCTCG 
GAGTCTGACG TCTCCTCATT TGAAATCCAG CATAGTGGAT TTGTGAAACA ACTGCTGCTT 
TATTTGACAT CTAAAAGTGA GAAAGATGCT GTAAGCAGGG ATATCAGATT GAAAAGATTT 
CTTCATGTAT TTTTCTCCTC TCCACTTCCT GGAGAAGAAC CCCTTGGAAG ATTAGAGCCA 
TTAGAAAATG CACCTTTGTT GGCATTAGTC CATAAAATGA ACAATTGCCT CAGTCAGATG 
GAGCAGTTTC CAGTGAAAGT GCATGACTTC CCCAGTGGAA ATGGAACAGG GAGCAGTTTT 
TCTCTTAACA GAGGATCCCA AGCTTTAAAA TTCTTCAATA CGCATCAGTT AAAATGCCAG 
CTGCAAAGAC ATCCAGACTG TGCTAATGTG AAACAGTGGA AGGGTGGACC TGTGAAGATT 
GATCCTCTGG CTTTGGTTCA AGCCATTGAA AGATATCTTG TTGTTAGAGG TTATGGAAGA 
GTTAGAGAAG ATGATGAGGA TAGTGATGAT GATGGGTCAG ATGAAGAAAT AGATGAATCC 
TTGGCTGCTC AATTCTTAAA TTCAGGGAAT GTGAGACATA GATTGCAGTT TTACATTGGG 
GATCACTTGC TACCATATAA TATGACTGTA TATCAAGCAG TTAAGCTGTA CAGTTTGCAA 
GCTGAGGAGG AGAGGGAATC GACTGATGAT GAAAGCAATC CATTAGGAAG AGCTGGGATT 
TGGACAAAAA CACATACCAT TTGGTACAAA CCTGTACGGG AGGATGAAGA TGGCAACAAA 
GACTGTGTTG GTGGTAAAAG AGGAAGAGCT CAAACTGCTC CCACAAAAAC CTCACCTAGG 
AATTCTAAAA AGCATGATGA GTTGTGGCAC GATGGTGTAT GCCCTTCAGT ATTGAATCCT 
TTAGAAGTTT ACCTCATATC TACTCCTCCT GAAAACATAA CCTTTGAAGA TCCTTCATTA 
GATGTTATTC TTCTTTTGAG AGTTTTACAT GCTATCAGTC GATACTGGTA TTATTTGTAT 
GATAATGCAA TATGCAAGGA GATTATTCCA ACTTCGGAAT TTATCAATAG TAAACTGACA 
GCAAAAGCAA ACAGACAGCT TCAAGATCCA CTGGTAATTA TGACAGGAAA CATACCGACT 
TGGCTGACAG AGCTTGGAAA AACATGCCCA TTTTTCTTTC CATTTGATAC CCGGCAAATG 
CTGTTTTATG TAACTGCTTT TGATCGTGAT CGAGCCATGC AAAGACTACT GGATACTAAT 
CCAGAAATCA ATCAATCAGA TTCTCAGGAT AGCAGAGTGG CACCACGACT GGACAGGAAA 
AAACGCACTG TGAACAGAGA TGAGCTGTTG AAACAGGCAG AATCTGTGAT GCAGGATCTA 
GGCAGTTCAA GAGCCATGTT GGAAATCCAG TATGAGAATG AAGTTGGCAC AGGCCTGGGC 
CCTACACTAG AGTTCTATGC ACTTGTATCT CAGGAACTAC AGAGAGCAGA CTTGGGCCTT 
TGGAGGGGAG AAGAAGTAAC TTTAGCCAAT CCAAAAGGAA GCCAAGAAGG TACCAAGTAT 
ATTCATAATC TCCAAGGCCT TTTTGCACTT CCTTTTGGTA GAACAGCCAA GCCTGCTCAC 
ATTGCAAAAG TTAAAATGAA GTTCCGCTTT CTGGGAAAAC TAATGGCTAA GGCAATCATG 
GATTTTAGAC TGGTGGATCT TCCTCTTGGA CTTCCTTTTT ATAAATGGAT GTTACGCCAG 
GAAACTTCTT TGACGTCACA TGACTTGTTC AGTATTGATC CAGTAGTAGC CAAATCAATA 
TATCACCTTG AAGATATTGT AAGACAAAAG AAAAGACTTG AGCAGGACAA AACACAGACC 
AAAGAAAGTC TACAGTATGC ATTGGAAACC CTGACTATGA ATGGCTGCTC AGTGGAGGAT 
CTAGGGCTGG ATTTCACACT TCCTGGATTT CCTAACATCG AGCTGAAAAA AGGGGGAAAA 
GATACACCAG TCACCATCCA CAACTTGGAG GAGTACCTCA GATTGGTTAT ATTCTGGGCA 
CTTAACGAAG GTGTTGCCAG ACAGTTTGAT TCTTTCAGAG ATGGATTTGA ATCAGTCTTC 
CCCCTCAGTC ATCTTCAATA CTTTTATCCT GAGGAGTTGG AGCAACTCTT ATGTGGCAGC 
AAAACAGACA CATGGGATGC CAAGACCTTA ATGGAATGTT GCAGGCCAGA TCACGGTTAT 
ACACATGACA GTCGAGCAGT GAAGTATCTC TTTGAAATTC TCAGTAGTTT TGATAGTGAG 
CAGCAGAGAC TGTTTCTTCA GTTTGTGACC GGTAGTCCCA GGCTGCCAGT AGGAGGCTTT 
CGAAGTTTGA ATCCTCCATT GACAATTGTA CGGAAGACAT TTGAGTCTAC AGAGAATCCA 
GATGACTTCT TGCCCTCAGT AATGACTTGT GTGAACTATC TCAAATTGCC GGACTATTCA 
ACTATTGAGA TAATGCGTGA AAAACTCTTG ATAGCTGCAA GAGAAGGGCA GCAGTCATTC 
CATCTTTCCT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015132457.1
CDS	150..6221
Translation	MSNRPNNNPGGSLRRSQRNTAGAQPQDDAAGGRGCSSSAVLIPQEDSERVSTSEKQKTGQVPKKDNSRGVKRSASPDYSRTNSPSSAKKPKALQHAETSSESNKPHTKSKRRHLDQEQQKSTQLPSTSKAHTRKGGAAGSSRSQKRKRTESTSCIKSRSAVESTGAEEKSAKSSKLASKSVTSAKAGCSTITDSSSSASTSSSSSAVASASSTVPQGARVKQGKDQNKARRSRSASSPSPRRSSRDKEQSKTGGSSKFDWAARFSPKVSLPKTKLSLPGSSKSETSKPGPSGLQAKLASLRKSTKKRSESPPAELPSLRRSTRQKTTGSCASTSRRGSGLGKRGAAEARRQEKMADPDNNQDGVNSSAARTDETPQGAAASSSVAGAVGMTTSGESESDDSEMGRLQALLEARGLPPHLFGPLGPRMSQLFHRTIGSGASSKAQQLLQGLQATDESQQLQAVIEMCQLLVMGNEETLGGFPVKSVVPALITLLQMEHNFDIMNHACRALTYMMEALPRSSAVVVDAIPVFLEKLQVIQCIDVAEQALTALEMLSRRHSKAILQAGGLADCLLYLEFFSINAQRNALAIAANCCQSITPDEFHFVADSLPLLTQRLTHQDKKSVESTCLCFARLVDNFQHEENLLQQVASKDLLTNIQQLLVVTPPILSSGMFIMVVRMFSLMCSNCPTLAVQLMKQNIAETLHFLLCGASNGSCQEQIDLVPRSPQELYELTSLICELMPCLPKEGIFAVDTMLKKGNAQNTDGAIWQWRDDRGLWHPYNRIDSRIIEAAHQVGEDEISLSTLGRVYTIDFNSMQQINEDTGTARAIQRKPNPLANTNTSGHSELKKDDARAQLMKEDPELAKSFIKTLFGVLYEVYSSSAGPAVRHKCLRAILRIIYFADAELLKDVLKNHAVSSHIASMLSSQDLKIVVGALQMAEILMQKLPDIFSVYFRREGVMHQVKNLAESEALLTSPPKVCTNGSGTLGTTTTISTGTATAATNAAADLGSPSLQHSREDSLDLSPQGRLSDVLKRKRLPKRGPRRPKYSPPRDDDKVDNQAKSPTTTQSPKSSFLASLNPKTWGRLSTQSNSNNIEPARTAGVSGLARAASKDTISNNREKIKGWIKEQAHKFVDRYFSSENMDGSNPALNVLQRLCTATEQLNLQVDGGTECLVEIRSIVSESDVSSFEIQHSGFVKQLLLYLTSKSEKDAVSRDIRLKRFLHVFFSSPLPGEEPLGRLEPLENAPLLALVHKMNNCLSQMEQFPVKVHDFPSGNGTGSSFSLNRGSQALKFFNTHQLKCQLQRHPDCANVKQWKGGPVKIDPLALVQAIERYLVVRGYGRVREDDEDSDDDGSDEEIDESLAAQFLNSGNVRHRLQFYIGDHLLPYNMTVYQAVKLYSLQAEEERESTDDESNPLGRAGIWTKTHTIWYKPVREDEDGNKDCVGGKRGRAQTAPTKTSPRNSKKHDELWHDGVCPSVLNPLEVYLISTPPENITFEDPSLDVILLLRVLHAISRYWYYLYDNAICKEIIPTSEFINSKLTAKANRQLQDPLVIMTGNIPTWLTELGKTCPFFFPFDTRQMLFYVTAFDRDRAMQRLLDTNPEINQSDSQDSRVAPRLDRKKRTVNRDELLKQAESVMQDLGSSRAMLEIQYENEVGTGLGPTLEFYALVSQELQRADLGLWRGEEVTLANPKGSQEGTKYIHNLQGLFALPFGRTAKPAHIAKVKMKFRFLGKLMAKAIMDFRLVDLPLGLPFYKWMLRQETSLTSHDLFSIDPVVAKSIYHLEDIVRQKKRLEQDKTQTKESLQYALETLTMNGCSVEDLGLDFTLPGFPNIELKKGGKDTPVTIHNLEEYLRLVIFWALNEGVARQFDSFRDGFESVFPLSHLQYFYPEELEQLLCGSKTDTWDAKTLMECCRPDHGYTHDSRAVKYLFEILSSFDSEQQRLFLQFVTGSPRLPVGGFRSLNPPLTIVRKTFESTENPDDFLPSVMTCVNYLKLPDYSTIEIMREKLLIAAREGQQSFHLS

Target ORF information:

RefSeq Version	XM_015276971.1
Organism	Gallus gallus(chicken)
Definition	Gallus gallus thyroid hormone receptor interactor 12 (TRIP12), transcript variant X10, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015276971.1

ORF Insert Sequence:

ATGTCCAACC GGCCTAATAA CAATCCAGGG GGGTCACTGC GACGTTCACA GAGGAACACT 
GCTGGGGCCC AGCCACAAGA CGACGCAGCA GGAGGAAGAG GCTGTAGTTC ATCTGCTGTT 
TTAATACCAC AAGAAGATTC AGAAAGAGTC AGTACTTCAG AAAAGCAAAA AACGGGGCAA 
GTGCCTAAGA AAGACAATTC TCGAGGAGTT AAACGCAGTG CTAGTCCAGA TTACAGCAGG 
ACCAATTCTC CTAGCTCTGC TAAAAAACCT AAAGCACTTC AACACGCCGA AACTTCCTCG 
GAAAGTAACA AGCCACATAC TAAATCCAAG AGAAGACACT TAGACCAAGA ACAGCAGAAG 
TCTACACAAT TGCCATCAAC AAGCAAGGCT CACACCAGAA AGGGTGGAGC TGCTGGTAGC 
TCCCGAAGTC AGAAAAGGAA AAGGACAGAG AGTACGTCTT GTATAAAGAG TCGTTCAGCA 
GTTGAATCAA CTGGAGCTGA AGAGAAGTCA GCAAAATCCT CCAAGCTGGC TTCAAAATCG 
GTGACCTCAG CCAAAGCTGG GTGTAGCACC ATCACTGATT CTTCTTCTTC AGCTTCTACA 
TCCTCTTCCT CTTCTGCTGT TGCCTCTGCT TCTTCCACTG TTCCTCAGGG TGCCAGAGTG 
AAACAGGGTA AGGACCAGAA TAAGGCTAGA CGTTCACGTT CTGCGTCCAG CCCTAGTCCA 
AGAAGGAGCA GCAGGGACAA AGAACAGAGC AAAACAGGTG GCTCTTCAAA GTTTGACTGG 
GCTGCTCGAT TCAGTCCAAA AGTTAGTCTC CCTAAAACAA AACTGTCTCT ACCAGGCTCT 
TCTAAGTCAG AGACCTCAAA ACCTGGACCT TCAGGACTAC AGGCTAAGCT AGCAAGCCTA 
AGAAAATCCA CAAAGAAGCG CAGTGAATCA CCACCTGCTG AGCTCCCCAG TTTACGGCGG 
AGCACACGGC AAAAGACCAC GGGCTCCTGT GCGAGTACCA GTCGGCGGGG CTCTGGCCTG 
GGCAAAAGAG GAGCAGCTGA AGCTCGCCGA CAGGAGAAGA TGGCTGATCC TGACAACAAC 
CAGGATGGAG TTAACTCTTC AGCTGCACGT ACAGATGAGA CTCCCCAGGG AGCTGCAGCT 
TCTAGCTCTG TTGCTGGGGC TGTAGGTATG ACAACATCTG GAGAAAGTGA GTCAGATGAC 
TCTGAGATGG GAAGACTACA AGCTCTGTTA GAGGCTAGGG GTCTTCCTCC TCACCTGTTT 
GGCCCTCTTG GTCCTCGGAT GTCGCAGCTC TTCCACAGGA CAATTGGAAG TGGAGCTAGT 
TCTAAAGCCC AGCAGCTTTT ACAAGGTCTC CAAGCCACTG ATGAAAGTCA GCAACTACAG 
GCAGTTATTG AGATGTGCCA GCTGTTGGTC ATGGGAAATG AAGAAACACT GGGAGGATTT 
CCAGTCAAGA GTGTTGTGCC AGCTTTGATA ACATTGCTGC AGATGGAGCA TAACTTTGAC 
ATCATGAACC ATGCTTGTCG AGCCTTAACA TACATGATGG AAGCACTTCC CAGATCATCT 
GCTGTAGTGG TAGATGCAAT TCCTGTCTTC TTGGAAAAGC TGCAAGTTAT TCAGTGCATT 
GATGTGGCAG AGCAGGCACT GACAGCCCTA GAGATGCTAT CACGCAGGCA TAGTAAAGCC 
ATTCTGCAGG CAGGTGGGTT GGCAGACTGC TTGCTGTATC TGGAATTCTT CAGTATAAAT 
GCGCAGAGGA ATGCACTAGC TATTGCTGCC AATTGCTGCC AGAGTATAAC ACCTGATGAG 
TTTCACTTTG TGGCAGACTC TTTGCCACTG CTTACACAAA GGTTAACCCA TCAGGACAAG 
AAGTCTGTTG AAAGCACTTG TCTCTGTTTT GCACGGCTGG TAGACAATTT CCAGCATGAG 
GAGAACTTGC TGCAGCAGGT TGCTTCCAAG GACTTGTTAA CAAACATCCA GCAACTCTTG 
GTAGTGACAC CTCCTATCCT GAGCTCAGGA ATGTTCATCA TGGTGGTGCG CATGTTTTCC 
TTAATGTGTT CCAATTGTCC AACGCTTGCA GTCCAACTTA TGAAGCAAAA CATTGCAGAA 
ACACTTCACT TTCTCCTTTG TGGAGCCTCA AATGGGAGTT GTCAAGAACA AATTGACCTT 
GTTCCACGAA GTCCTCAAGA ACTCTATGAG CTTACTTCTC TTATCTGTGA ACTGATGCCT 
TGTCTGCCAA AAGAGGGGAT ATTTGCTGTT GATACTATGC TAAAGAAAGG AAATGCACAA 
AATACAGATG GTGCGATATG GCAATGGCGA GATGACAGGG GTCTCTGGCA TCCCTATAAC 
AGGATTGATA GTCGAATAAT AGAGGCAGCT CATCAGGTTG GTGAGGATGA GATAAGCCTG 
TCGACACTTG GACGTGTCTA TACTATTGAT TTTAATTCTA TGCAGCAAAT AAATGAGGAT 
ACTGGAACAG CACGTGCCAT TCAGCGGAAA CCAAACCCTT TAGCCAATAC AAACACTAGT 
GGACATTCAG AATTGAAGAA GGATGATGCT CGAGCACAGC TAATGAAAGA AGACCCAGAA 
TTGGCAAAAT CCTTTATCAA AACACTGTTT GGTGTTCTTT ATGAAGTGTA TAGTTCTTCA 
GCTGGACCTG CTGTTAGACA CAAGTGCCTT AGAGCAATAC TTAGAATAAT TTATTTTGCT 
GATGCTGAAC TCCTGAAGGA TGTGCTGAAA AACCATGCTG TGTCAAGTCA CATTGCCTCC 
ATGCTGTCAA GTCAAGACCT TAAGATAGTA GTTGGAGCCC TGCAGATGGC AGAAATCTTA 
ATGCAGAAGC TACCTGACAT TTTTAGTGTT TACTTCAGAA GAGAAGGAGT GATGCATCAA 
GTGAAAAACT TAGCGGAGTC TGAGGCTTTG CTAACGAGCC CACCAAAAGT ATGCACTAAT 
GGATCAGGAA CACTGGGTAC CACTACAACT ATAAGTACTG GAACAGCCAC TGCTGCCACT 
AATGCAGCTG CAGATTTGGG CTCTCCCAGC TTACAACACA GCCGGGAGGA TTCTTTAGAT 
CTGAGCCCAC AGGGACGACT GAGTGATGTT CTGAAGAGAA AAAGACTGCC AAAACGAGGG 
CCAAGGAGAC CAAAATATTC TCCCCCAAGA GATGATGACA AAGTAGACAA TCAAGCAAAA 
AGCCCCACGA CTACTCAGTC TCCTAAATCT TCTTTCTTAG CAAGTTTAAA TCCCAAAACA 
TGGGGAAGAT TAAGTACACA GTCTAACAGT AATAACATTG AACCAGCAAG AACAGCAGGT 
GTAAGTGGTC TTGCAAGAGC TGCTTCTAAG GATACAATTT CCAATAACAG AGAAAAAATT 
AAGGGTTGGA TTAAGGAGCA AGCCCATAAA TTTGTAGACC GTTATTTTAG TTCGGAAAAC 
ATGGATGGAA GCAATCCTGC ACTAAATGTA TTACAGAGAC TTTGCACTGC AACTGAACAA 
CTTAACCTCC AGGTGGATGG TGGAACAGAG TGCCTTGTAG AAATCCGTAG CATTGTCTCG 
GAGTCTGACG TCTCCTCATT TGAAATCCAG CATAGTGGAT TTGTGAAACA ACTGCTGCTT 
TATTTGACAT CTAAAAGTGA GAAAGATGCT GTAAGCAGGG ATATCAGATT GAAAAGATTT 
CTTCATGTAT TTTTCTCCTC TCCACTTCCT GGAGAAGAAC CCCTTGGAAG ATTAGAGCCA 
TTAGAAAATG CACCTTTGTT GGCATTAGTC CATAAAATGA ACAATTGCCT CAGTCAGATG 
GAGCAGTTTC CAGTGAAAGT GCATGACTTC CCCAGTGGAA ATGGAACAGG GAGCAGTTTT 
TCTCTTAACA GAGGATCCCA AGCTTTAAAA TTCTTCAATA CGCATCAGTT AAAATGCCAG 
CTGCAAAGAC ATCCAGACTG TGCTAATGTG AAACAGTGGA AGGGTGGACC TGTGAAGATT 
GATCCTCTGG CTTTGGTTCA AGCCATTGAA AGATATCTTG TTGTTAGAGG TTATGGAAGA 
GTTAGAGAAG ATGATGAGGA TAGTGATGAT GATGGGTCAG ATGAAGAAAT AGATGAATCC 
TTGGCTGCTC AATTCTTAAA TTCAGGGAAT GTGAGACATA GATTGCAGTT TTACATTGGG 
GATCACTTGC TACCATATAA TATGACTGTA TATCAAGCAG TTAAGCTGTA CAGTTTGCAA 
GCTGAGGAGG AGAGGGAATC GACTGATGAT GAAAGCAATC CATTAGGAAG AGCTGGGATT 
TGGACAAAAA CACATACCAT TTGGTACAAA CCTGTACGGG AGGATGAAGA TGGCAACAAA 
GACTGTGTTG GTGGTAAAAG AGGAAGAGCT CAAACTGCTC CCACAAAAAC CTCACCTAGG 
AATTCTAAAA AGCATGATGA GTTGTGGCAC GATGGTGTAT GCCCTTCAGT ATTGAATCCT 
TTAGAAGTTT ACCTCATATC TACTCCTCCT GAAAACATAA CCTTTGAAGA TCCTTCATTA 
GATGTTATTC TTCTTTTGAG AGTTTTACAT GCTATCAGTC GATACTGGTA TTATTTGTAT 
GATAATGCAA TATGCAAGGA GATTATTCCA ACTTCGGAAT TTATCAATAG TAAACTGACA 
GCAAAAGCAA ACAGACAGCT TCAAGATCCA CTGGTAATTA TGACAGGAAA CATACCGACT 
TGGCTGACAG AGCTTGGAAA AACATGCCCA TTTTTCTTTC CATTTGATAC CCGGCAAATG 
CTGTTTTATG TAACTGCTTT TGATCGTGAT CGAGCCATGC AAAGACTACT GGATACTAAT 
CCAGAAATCA ATCAATCAGA TTCTCAGGAT AGCAGAGTGG CACCACGACT GGACAGGAAA 
AAACGCACTG TGAACAGAGA TGAGCTGTTG AAACAGGCAG AATCTGTGAT GCAGGATCTA 
GGCAGTTCAA GAGCCATGTT GGAAATCCAG TATGAGAATG AAGTTGGCAC AGGCCTGGGC 
CCTACACTAG AGTTCTATGC ACTTGTATCT CAGGAACTAC AGAGAGCAGA CTTGGGCCTT 
TGGAGGGGAG AAGAAGTAAC TTTAGCCAAT CCAAAAGGAA GCCAAGAAGG TACCAAGTAT 
ATTCATAATC TCCAAGGCCT TTTTGCACTT CCTTTTGGTA GAACAGCCAA GCCTGCTCAC 
ATTGCAAAAG TTAAAATGAA GTTCCGCTTT CTGGGAAAAC TAATGGCTAA GGCAATCATG 
GATTTTAGAC TGGTGGATCT TCCTCTTGGA CTTCCTTTTT ATAAATGGAT GTTACGCCAG 
GAAACTTCTT TGACGTCACA TGACTTGTTC AGTATTGATC CAGTAGTAGC CAAATCAATA 
TATCACCTTG AAGATATTGT AAGACAAAAG AAAAGACTTG AGCAGGACAA AACACAGACC 
AAAGAAAGTC TACAGTATGC ATTGGAAACC CTGACTATGA ATGGCTGCTC AGTGGAGGAT 
CTAGGGCTGG ATTTCACACT TCCTGGATTT CCTAACATCG AGCTGAAAAA AGGGGGAAAA 
GATACACCAG TCACCATCCA CAACTTGGAG GAGTACCTCA GATTGGTTAT ATTCTGGGCA 
CTTAACGAAG GTGTTGCCAG ACAGTTTGAT TCTTTCAGAG ATGGATTTGA ATCAGTCTTC 
CCCCTCAGTC ATCTTCAATA CTTTTATCCT GAGGAGTTGG AGCAACTCTT ATGTGGCAGC 
AAAACAGACA CATGGGATGC CAAGACCTTA ATGGAATGTT GCAGGCCAGA TCACGGTTAT 
ACACATGACA GTCGAGCAGT GAAGTATCTC TTTGAAATTC TCAGTAGTTT TGATAGTGAG 
CAGCAGAGAC TGTTTCTTCA GTTTGTGACC GGTAGTCCCA GGCTGCCAGT AGGAGGCTTT 
CGAAGTTTGA ATCCTCCATT GACAATTGTA CGGAAGACAT TTGAGTCTAC AGAGAATCCA 
GATGACTTCT TGCCCTCAGT AATGACTTGT GTGAACTATC TCAAATTGCC GGACTATTCA 
ACTATTGAGA TAATGCGTGA AAAACTCTTG ATAGCTGCAA GAGAAGGGCA GCAGTCATTC 
CATCTTTCCT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TRIP12 ( XM_015276971.2 ) cDNA ORF clone, Gallus gallus(chicken) -> XP_015132457.1 Gallus gallus thyroid hormone receptor interactor 12 (TRIP12), transcript variant X9, mRNA.

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CloneID	OGa12504
Clone ID Related Accession (Same CDS sequence)	XM_015276971.1 , XM_015276971.2
Accession Version	XM_015276971.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6072bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-16
Organism	Gallus gallus(chicken)
Product	E3 ubiquitin-protein ligase TRIP12 isoform X5
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006096.5) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On May 17, 2018 this sequence version replaced XM_015276971.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Gallus gallus Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCCAACC GGCCTAATAA CAATCCAGGG GGGTCACTGC GACGTTCACA GAGGAACACT 
GCTGGGGCCC AGCCACAAGA CGACGCAGCA GGAGGAAGAG GCTGTAGTTC ATCTGCTGTT 
TTAATACCAC AAGAAGATTC AGAAAGAGTC AGTACTTCAG AAAAGCAAAA AACGGGGCAA 
GTGCCTAAGA AAGACAATTC TCGAGGAGTT AAACGCAGTG CTAGTCCAGA TTACAGCAGG 
ACCAATTCTC CTAGCTCTGC TAAAAAACCT AAAGCACTTC AACACGCCGA AACTTCCTCG 
GAAAGTAACA AGCCACATAC TAAATCCAAG AGAAGACACT TAGACCAAGA ACAGCAGAAG 
TCTACACAAT TGCCATCAAC AAGCAAGGCT CACACCAGAA AGGGTGGAGC TGCTGGTAGC 
TCCCGAAGTC AGAAAAGGAA AAGGACAGAG AGTACGTCTT GTATAAAGAG TCGTTCAGCA 
GTTGAATCAA CTGGAGCTGA AGAGAAGTCA GCAAAATCCT CCAAGCTGGC TTCAAAATCG 
GTGACCTCAG CCAAAGCTGG GTGTAGCACC ATCACTGATT CTTCTTCTTC AGCTTCTACA 
TCCTCTTCCT CTTCTGCTGT TGCCTCTGCT TCTTCCACTG TTCCTCAGGG TGCCAGAGTG 
AAACAGGGTA AGGACCAGAA TAAGGCTAGA CGTTCACGTT CTGCGTCCAG CCCTAGTCCA 
AGAAGGAGCA GCAGGGACAA AGAACAGAGC AAAACAGGTG GCTCTTCAAA GTTTGACTGG 
GCTGCTCGAT TCAGTCCAAA AGTTAGTCTC CCTAAAACAA AACTGTCTCT ACCAGGCTCT 
TCTAAGTCAG AGACCTCAAA ACCTGGACCT TCAGGACTAC AGGCTAAGCT AGCAAGCCTA 
AGAAAATCCA CAAAGAAGCG CAGTGAATCA CCACCTGCTG AGCTCCCCAG TTTACGGCGG 
AGCACACGGC AAAAGACCAC GGGCTCCTGT GCGAGTACCA GTCGGCGGGG CTCTGGCCTG 
GGCAAAAGAG GAGCAGCTGA AGCTCGCCGA CAGGAGAAGA TGGCTGATCC TGACAACAAC 
CAGGATGGAG TTAACTCTTC AGCTGCACGT ACAGATGAGA CTCCCCAGGG AGCTGCAGCT 
TCTAGCTCTG TTGCTGGGGC TGTAGGTATG ACAACATCTG GAGAAAGTGA GTCAGATGAC 
TCTGAGATGG GAAGACTACA AGCTCTGTTA GAGGCTAGGG GTCTTCCTCC TCACCTGTTT 
GGCCCTCTTG GTCCTCGGAT GTCGCAGCTC TTCCACAGGA CAATTGGAAG TGGAGCTAGT 
TCTAAAGCCC AGCAGCTTTT ACAAGGTCTC CAAGCCACTG ATGAAAGTCA GCAACTACAG 
GCAGTTATTG AGATGTGCCA GCTGTTGGTC ATGGGAAATG AAGAAACACT GGGAGGATTT 
CCAGTCAAGA GTGTTGTGCC AGCTTTGATA ACATTGCTGC AGATGGAGCA TAACTTTGAC 
ATCATGAACC ATGCTTGTCG AGCCTTAACA TACATGATGG AAGCACTTCC CAGATCATCT 
GCTGTAGTGG TAGATGCAAT TCCTGTCTTC TTGGAAAAGC TGCAAGTTAT TCAGTGCATT 
GATGTGGCAG AGCAGGCACT GACAGCCCTA GAGATGCTAT CACGCAGGCA TAGTAAAGCC 
ATTCTGCAGG CAGGTGGGTT GGCAGACTGC TTGCTGTATC TGGAATTCTT CAGTATAAAT 
GCGCAGAGGA ATGCACTAGC TATTGCTGCC AATTGCTGCC AGAGTATAAC ACCTGATGAG 
TTTCACTTTG TGGCAGACTC TTTGCCACTG CTTACACAAA GGTTAACCCA TCAGGACAAG 
AAGTCTGTTG AAAGCACTTG TCTCTGTTTT GCACGGCTGG TAGACAATTT CCAGCATGAG 
GAGAACTTGC TGCAGCAGGT TGCTTCCAAG GACTTGTTAA CAAACATCCA GCAACTCTTG 
GTAGTGACAC CTCCTATCCT GAGCTCAGGA ATGTTCATCA TGGTGGTGCG CATGTTTTCC 
TTAATGTGTT CCAATTGTCC AACGCTTGCA GTCCAACTTA TGAAGCAAAA CATTGCAGAA 
ACACTTCACT TTCTCCTTTG TGGAGCCTCA AATGGGAGTT GTCAAGAACA AATTGACCTT 
GTTCCACGAA GTCCTCAAGA ACTCTATGAG CTTACTTCTC TTATCTGTGA ACTGATGCCT 
TGTCTGCCAA AAGAGGGGAT ATTTGCTGTT GATACTATGC TAAAGAAAGG AAATGCACAA 
AATACAGATG GTGCGATATG GCAATGGCGA GATGACAGGG GTCTCTGGCA TCCCTATAAC 
AGGATTGATA GTCGAATAAT AGAGGCAGCT CATCAGGTTG GTGAGGATGA GATAAGCCTG 
TCGACACTTG GACGTGTCTA TACTATTGAT TTTAATTCTA TGCAGCAAAT AAATGAGGAT 
ACTGGAACAG CACGTGCCAT TCAGCGGAAA CCAAACCCTT TAGCCAATAC AAACACTAGT 
GGACATTCAG AATTGAAGAA GGATGATGCT CGAGCACAGC TAATGAAAGA AGACCCAGAA 
TTGGCAAAAT CCTTTATCAA AACACTGTTT GGTGTTCTTT ATGAAGTGTA TAGTTCTTCA 
GCTGGACCTG CTGTTAGACA CAAGTGCCTT AGAGCAATAC TTAGAATAAT TTATTTTGCT 
GATGCTGAAC TCCTGAAGGA TGTGCTGAAA AACCATGCTG TGTCAAGTCA CATTGCCTCC 
ATGCTGTCAA GTCAAGACCT TAAGATAGTA GTTGGAGCCC TGCAGATGGC AGAAATCTTA 
ATGCAGAAGC TACCTGACAT TTTTAGTGTT TACTTCAGAA GAGAAGGAGT GATGCATCAA 
GTGAAAAACT TAGCGGAGTC TGAGGCTTTG CTAACGAGCC CACCAAAAGT ATGCACTAAT 
GGATCAGGAA CACTGGGTAC CACTACAACT ATAAGTACTG GAACAGCCAC TGCTGCCACT 
AATGCAGCTG CAGATTTGGG CTCTCCCAGC TTACAACACA GCCGGGAGGA TTCTTTAGAT 
CTGAGCCCAC AGGGACGACT GAGTGATGTT CTGAAGAGAA AAAGACTGCC AAAACGAGGG 
CCAAGGAGAC CAAAATATTC TCCCCCAAGA GATGATGACA AAGTAGACAA TCAAGCAAAA 
AGCCCCACGA CTACTCAGTC TCCTAAATCT TCTTTCTTAG CAAGTTTAAA TCCCAAAACA 
TGGGGAAGAT TAAGTACACA GTCTAACAGT AATAACATTG AACCAGCAAG AACAGCAGGT 
GTAAGTGGTC TTGCAAGAGC TGCTTCTAAG GATACAATTT CCAATAACAG AGAAAAAATT 
AAGGGTTGGA TTAAGGAGCA AGCCCATAAA TTTGTAGACC GTTATTTTAG TTCGGAAAAC 
ATGGATGGAA GCAATCCTGC ACTAAATGTA TTACAGAGAC TTTGCACTGC AACTGAACAA 
CTTAACCTCC AGGTGGATGG TGGAACAGAG TGCCTTGTAG AAATCCGTAG CATTGTCTCG 
GAGTCTGACG TCTCCTCATT TGAAATCCAG CATAGTGGAT TTGTGAAACA ACTGCTGCTT 
TATTTGACAT CTAAAAGTGA GAAAGATGCT GTAAGCAGGG ATATCAGATT GAAAAGATTT 
CTTCATGTAT TTTTCTCCTC TCCACTTCCT GGAGAAGAAC CCCTTGGAAG ATTAGAGCCA 
TTAGAAAATG CACCTTTGTT GGCATTAGTC CATAAAATGA ACAATTGCCT CAGTCAGATG 
GAGCAGTTTC CAGTGAAAGT GCATGACTTC CCCAGTGGAA ATGGAACAGG GAGCAGTTTT 
TCTCTTAACA GAGGATCCCA AGCTTTAAAA TTCTTCAATA CGCATCAGTT AAAATGCCAG 
CTGCAAAGAC ATCCAGACTG TGCTAATGTG AAACAGTGGA AGGGTGGACC TGTGAAGATT 
GATCCTCTGG CTTTGGTTCA AGCCATTGAA AGATATCTTG TTGTTAGAGG TTATGGAAGA 
GTTAGAGAAG ATGATGAGGA TAGTGATGAT GATGGGTCAG ATGAAGAAAT AGATGAATCC 
TTGGCTGCTC AATTCTTAAA TTCAGGGAAT GTGAGACATA GATTGCAGTT TTACATTGGG 
GATCACTTGC TACCATATAA TATGACTGTA TATCAAGCAG TTAAGCTGTA CAGTTTGCAA 
GCTGAGGAGG AGAGGGAATC GACTGATGAT GAAAGCAATC CATTAGGAAG AGCTGGGATT 
TGGACAAAAA CACATACCAT TTGGTACAAA CCTGTACGGG AGGATGAAGA TGGCAACAAA 
GACTGTGTTG GTGGTAAAAG AGGAAGAGCT CAAACTGCTC CCACAAAAAC CTCACCTAGG 
AATTCTAAAA AGCATGATGA GTTGTGGCAC GATGGTGTAT GCCCTTCAGT ATTGAATCCT 
TTAGAAGTTT ACCTCATATC TACTCCTCCT GAAAACATAA CCTTTGAAGA TCCTTCATTA 
GATGTTATTC TTCTTTTGAG AGTTTTACAT GCTATCAGTC GATACTGGTA TTATTTGTAT 
GATAATGCAA TATGCAAGGA GATTATTCCA ACTTCGGAAT TTATCAATAG TAAACTGACA 
GCAAAAGCAA ACAGACAGCT TCAAGATCCA CTGGTAATTA TGACAGGAAA CATACCGACT 
TGGCTGACAG AGCTTGGAAA AACATGCCCA TTTTTCTTTC CATTTGATAC CCGGCAAATG 
CTGTTTTATG TAACTGCTTT TGATCGTGAT CGAGCCATGC AAAGACTACT GGATACTAAT 
CCAGAAATCA ATCAATCAGA TTCTCAGGAT AGCAGAGTGG CACCACGACT GGACAGGAAA 
AAACGCACTG TGAACAGAGA TGAGCTGTTG AAACAGGCAG AATCTGTGAT GCAGGATCTA 
GGCAGTTCAA GAGCCATGTT GGAAATCCAG TATGAGAATG AAGTTGGCAC AGGCCTGGGC 
CCTACACTAG AGTTCTATGC ACTTGTATCT CAGGAACTAC AGAGAGCAGA CTTGGGCCTT 
TGGAGGGGAG AAGAAGTAAC TTTAGCCAAT CCAAAAGGAA GCCAAGAAGG TACCAAGTAT 
ATTCATAATC TCCAAGGCCT TTTTGCACTT CCTTTTGGTA GAACAGCCAA GCCTGCTCAC 
ATTGCAAAAG TTAAAATGAA GTTCCGCTTT CTGGGAAAAC TAATGGCTAA GGCAATCATG 
GATTTTAGAC TGGTGGATCT TCCTCTTGGA CTTCCTTTTT ATAAATGGAT GTTACGCCAG 
GAAACTTCTT TGACGTCACA TGACTTGTTC AGTATTGATC CAGTAGTAGC CAAATCAATA 
TATCACCTTG AAGATATTGT AAGACAAAAG AAAAGACTTG AGCAGGACAA AACACAGACC 
AAAGAAAGTC TACAGTATGC ATTGGAAACC CTGACTATGA ATGGCTGCTC AGTGGAGGAT 
CTAGGGCTGG ATTTCACACT TCCTGGATTT CCTAACATCG AGCTGAAAAA AGGGGGAAAA 
GATACACCAG TCACCATCCA CAACTTGGAG GAGTACCTCA GATTGGTTAT ATTCTGGGCA 
CTTAACGAAG GTGTTGCCAG ACAGTTTGAT TCTTTCAGAG ATGGATTTGA ATCAGTCTTC 
CCCCTCAGTC ATCTTCAATA CTTTTATCCT GAGGAGTTGG AGCAACTCTT ATGTGGCAGC 
AAAACAGACA CATGGGATGC CAAGACCTTA ATGGAATGTT GCAGGCCAGA TCACGGTTAT 
ACACATGACA GTCGAGCAGT GAAGTATCTC TTTGAAATTC TCAGTAGTTT TGATAGTGAG 
CAGCAGAGAC TGTTTCTTCA GTTTGTGACC GGTAGTCCCA GGCTGCCAGT AGGAGGCTTT 
CGAAGTTTGA ATCCTCCATT GACAATTGTA CGGAAGACAT TTGAGTCTAC AGAGAATCCA 
GATGACTTCT TGCCCTCAGT AATGACTTGT GTGAACTATC TCAAATTGCC GGACTATTCA 
ACTATTGAGA TAATGCGTGA AAAACTCTTG ATAGCTGCAA GAGAAGGGCA GCAGTCATTC 
CATCTTTCCT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015132457.1
CDS	138..6209
Translation	MSNRPNNNPGGSLRRSQRNTAGAQPQDDAAGGRGCSSSAVLIPQEDSERVSTSEKQKTGQVPKKDNSRGVKRSASPDYSRTNSPSSAKKPKALQHAETSSESNKPHTKSKRRHLDQEQQKSTQLPSTSKAHTRKGGAAGSSRSQKRKRTESTSCIKSRSAVESTGAEEKSAKSSKLASKSVTSAKAGCSTITDSSSSASTSSSSSAVASASSTVPQGARVKQGKDQNKARRSRSASSPSPRRSSRDKEQSKTGGSSKFDWAARFSPKVSLPKTKLSLPGSSKSETSKPGPSGLQAKLASLRKSTKKRSESPPAELPSLRRSTRQKTTGSCASTSRRGSGLGKRGAAEARRQEKMADPDNNQDGVNSSAARTDETPQGAAASSSVAGAVGMTTSGESESDDSEMGRLQALLEARGLPPHLFGPLGPRMSQLFHRTIGSGASSKAQQLLQGLQATDESQQLQAVIEMCQLLVMGNEETLGGFPVKSVVPALITLLQMEHNFDIMNHACRALTYMMEALPRSSAVVVDAIPVFLEKLQVIQCIDVAEQALTALEMLSRRHSKAILQAGGLADCLLYLEFFSINAQRNALAIAANCCQSITPDEFHFVADSLPLLTQRLTHQDKKSVESTCLCFARLVDNFQHEENLLQQVASKDLLTNIQQLLVVTPPILSSGMFIMVVRMFSLMCSNCPTLAVQLMKQNIAETLHFLLCGASNGSCQEQIDLVPRSPQELYELTSLICELMPCLPKEGIFAVDTMLKKGNAQNTDGAIWQWRDDRGLWHPYNRIDSRIIEAAHQVGEDEISLSTLGRVYTIDFNSMQQINEDTGTARAIQRKPNPLANTNTSGHSELKKDDARAQLMKEDPELAKSFIKTLFGVLYEVYSSSAGPAVRHKCLRAILRIIYFADAELLKDVLKNHAVSSHIASMLSSQDLKIVVGALQMAEILMQKLPDIFSVYFRREGVMHQVKNLAESEALLTSPPKVCTNGSGTLGTTTTISTGTATAATNAAADLGSPSLQHSREDSLDLSPQGRLSDVLKRKRLPKRGPRRPKYSPPRDDDKVDNQAKSPTTTQSPKSSFLASLNPKTWGRLSTQSNSNNIEPARTAGVSGLARAASKDTISNNREKIKGWIKEQAHKFVDRYFSSENMDGSNPALNVLQRLCTATEQLNLQVDGGTECLVEIRSIVSESDVSSFEIQHSGFVKQLLLYLTSKSEKDAVSRDIRLKRFLHVFFSSPLPGEEPLGRLEPLENAPLLALVHKMNNCLSQMEQFPVKVHDFPSGNGTGSSFSLNRGSQALKFFNTHQLKCQLQRHPDCANVKQWKGGPVKIDPLALVQAIERYLVVRGYGRVREDDEDSDDDGSDEEIDESLAAQFLNSGNVRHRLQFYIGDHLLPYNMTVYQAVKLYSLQAEEERESTDDESNPLGRAGIWTKTHTIWYKPVREDEDGNKDCVGGKRGRAQTAPTKTSPRNSKKHDELWHDGVCPSVLNPLEVYLISTPPENITFEDPSLDVILLLRVLHAISRYWYYLYDNAICKEIIPTSEFINSKLTAKANRQLQDPLVIMTGNIPTWLTELGKTCPFFFPFDTRQMLFYVTAFDRDRAMQRLLDTNPEINQSDSQDSRVAPRLDRKKRTVNRDELLKQAESVMQDLGSSRAMLEIQYENEVGTGLGPTLEFYALVSQELQRADLGLWRGEEVTLANPKGSQEGTKYIHNLQGLFALPFGRTAKPAHIAKVKMKFRFLGKLMAKAIMDFRLVDLPLGLPFYKWMLRQETSLTSHDLFSIDPVVAKSIYHLEDIVRQKKRLEQDKTQTKESLQYALETLTMNGCSVEDLGLDFTLPGFPNIELKKGGKDTPVTIHNLEEYLRLVIFWALNEGVARQFDSFRDGFESVFPLSHLQYFYPEELEQLLCGSKTDTWDAKTLMECCRPDHGYTHDSRAVKYLFEILSSFDSEQQRLFLQFVTGSPRLPVGGFRSLNPPLTIVRKTFESTENPDDFLPSVMTCVNYLKLPDYSTIEIMREKLLIAAREGQQSFHLS

Target ORF information:

RefSeq Version	XM_015276971.2
Organism	Gallus gallus(chicken)
Definition	Gallus gallus thyroid hormone receptor interactor 12 (TRIP12), transcript variant X9, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015276971.2

ORF Insert Sequence:

ATGTCCAACC GGCCTAATAA CAATCCAGGG GGGTCACTGC GACGTTCACA GAGGAACACT 
GCTGGGGCCC AGCCACAAGA CGACGCAGCA GGAGGAAGAG GCTGTAGTTC ATCTGCTGTT 
TTAATACCAC AAGAAGATTC AGAAAGAGTC AGTACTTCAG AAAAGCAAAA AACGGGGCAA 
GTGCCTAAGA AAGACAATTC TCGAGGAGTT AAACGCAGTG CTAGTCCAGA TTACAGCAGG 
ACCAATTCTC CTAGCTCTGC TAAAAAACCT AAAGCACTTC AACACGCCGA AACTTCCTCG 
GAAAGTAACA AGCCACATAC TAAATCCAAG AGAAGACACT TAGACCAAGA ACAGCAGAAG 
TCTACACAAT TGCCATCAAC AAGCAAGGCT CACACCAGAA AGGGTGGAGC TGCTGGTAGC 
TCCCGAAGTC AGAAAAGGAA AAGGACAGAG AGTACGTCTT GTATAAAGAG TCGTTCAGCA 
GTTGAATCAA CTGGAGCTGA AGAGAAGTCA GCAAAATCCT CCAAGCTGGC TTCAAAATCG 
GTGACCTCAG CCAAAGCTGG GTGTAGCACC ATCACTGATT CTTCTTCTTC AGCTTCTACA 
TCCTCTTCCT CTTCTGCTGT TGCCTCTGCT TCTTCCACTG TTCCTCAGGG TGCCAGAGTG 
AAACAGGGTA AGGACCAGAA TAAGGCTAGA CGTTCACGTT CTGCGTCCAG CCCTAGTCCA 
AGAAGGAGCA GCAGGGACAA AGAACAGAGC AAAACAGGTG GCTCTTCAAA GTTTGACTGG 
GCTGCTCGAT TCAGTCCAAA AGTTAGTCTC CCTAAAACAA AACTGTCTCT ACCAGGCTCT 
TCTAAGTCAG AGACCTCAAA ACCTGGACCT TCAGGACTAC AGGCTAAGCT AGCAAGCCTA 
AGAAAATCCA CAAAGAAGCG CAGTGAATCA CCACCTGCTG AGCTCCCCAG TTTACGGCGG 
AGCACACGGC AAAAGACCAC GGGCTCCTGT GCGAGTACCA GTCGGCGGGG CTCTGGCCTG 
GGCAAAAGAG GAGCAGCTGA AGCTCGCCGA CAGGAGAAGA TGGCTGATCC TGACAACAAC 
CAGGATGGAG TTAACTCTTC AGCTGCACGT ACAGATGAGA CTCCCCAGGG AGCTGCAGCT 
TCTAGCTCTG TTGCTGGGGC TGTAGGTATG ACAACATCTG GAGAAAGTGA GTCAGATGAC 
TCTGAGATGG GAAGACTACA AGCTCTGTTA GAGGCTAGGG GTCTTCCTCC TCACCTGTTT 
GGCCCTCTTG GTCCTCGGAT GTCGCAGCTC TTCCACAGGA CAATTGGAAG TGGAGCTAGT 
TCTAAAGCCC AGCAGCTTTT ACAAGGTCTC CAAGCCACTG ATGAAAGTCA GCAACTACAG 
GCAGTTATTG AGATGTGCCA GCTGTTGGTC ATGGGAAATG AAGAAACACT GGGAGGATTT 
CCAGTCAAGA GTGTTGTGCC AGCTTTGATA ACATTGCTGC AGATGGAGCA TAACTTTGAC 
ATCATGAACC ATGCTTGTCG AGCCTTAACA TACATGATGG AAGCACTTCC CAGATCATCT 
GCTGTAGTGG TAGATGCAAT TCCTGTCTTC TTGGAAAAGC TGCAAGTTAT TCAGTGCATT 
GATGTGGCAG AGCAGGCACT GACAGCCCTA GAGATGCTAT CACGCAGGCA TAGTAAAGCC 
ATTCTGCAGG CAGGTGGGTT GGCAGACTGC TTGCTGTATC TGGAATTCTT CAGTATAAAT 
GCGCAGAGGA ATGCACTAGC TATTGCTGCC AATTGCTGCC AGAGTATAAC ACCTGATGAG 
TTTCACTTTG TGGCAGACTC TTTGCCACTG CTTACACAAA GGTTAACCCA TCAGGACAAG 
AAGTCTGTTG AAAGCACTTG TCTCTGTTTT GCACGGCTGG TAGACAATTT CCAGCATGAG 
GAGAACTTGC TGCAGCAGGT TGCTTCCAAG GACTTGTTAA CAAACATCCA GCAACTCTTG 
GTAGTGACAC CTCCTATCCT GAGCTCAGGA ATGTTCATCA TGGTGGTGCG CATGTTTTCC 
TTAATGTGTT CCAATTGTCC AACGCTTGCA GTCCAACTTA TGAAGCAAAA CATTGCAGAA 
ACACTTCACT TTCTCCTTTG TGGAGCCTCA AATGGGAGTT GTCAAGAACA AATTGACCTT 
GTTCCACGAA GTCCTCAAGA ACTCTATGAG CTTACTTCTC TTATCTGTGA ACTGATGCCT 
TGTCTGCCAA AAGAGGGGAT ATTTGCTGTT GATACTATGC TAAAGAAAGG AAATGCACAA 
AATACAGATG GTGCGATATG GCAATGGCGA GATGACAGGG GTCTCTGGCA TCCCTATAAC 
AGGATTGATA GTCGAATAAT AGAGGCAGCT CATCAGGTTG GTGAGGATGA GATAAGCCTG 
TCGACACTTG GACGTGTCTA TACTATTGAT TTTAATTCTA TGCAGCAAAT AAATGAGGAT 
ACTGGAACAG CACGTGCCAT TCAGCGGAAA CCAAACCCTT TAGCCAATAC AAACACTAGT 
GGACATTCAG AATTGAAGAA GGATGATGCT CGAGCACAGC TAATGAAAGA AGACCCAGAA 
TTGGCAAAAT CCTTTATCAA AACACTGTTT GGTGTTCTTT ATGAAGTGTA TAGTTCTTCA 
GCTGGACCTG CTGTTAGACA CAAGTGCCTT AGAGCAATAC TTAGAATAAT TTATTTTGCT 
GATGCTGAAC TCCTGAAGGA TGTGCTGAAA AACCATGCTG TGTCAAGTCA CATTGCCTCC 
ATGCTGTCAA GTCAAGACCT TAAGATAGTA GTTGGAGCCC TGCAGATGGC AGAAATCTTA 
ATGCAGAAGC TACCTGACAT TTTTAGTGTT TACTTCAGAA GAGAAGGAGT GATGCATCAA 
GTGAAAAACT TAGCGGAGTC TGAGGCTTTG CTAACGAGCC CACCAAAAGT ATGCACTAAT 
GGATCAGGAA CACTGGGTAC CACTACAACT ATAAGTACTG GAACAGCCAC TGCTGCCACT 
AATGCAGCTG CAGATTTGGG CTCTCCCAGC TTACAACACA GCCGGGAGGA TTCTTTAGAT 
CTGAGCCCAC AGGGACGACT GAGTGATGTT CTGAAGAGAA AAAGACTGCC AAAACGAGGG 
CCAAGGAGAC CAAAATATTC TCCCCCAAGA GATGATGACA AAGTAGACAA TCAAGCAAAA 
AGCCCCACGA CTACTCAGTC TCCTAAATCT TCTTTCTTAG CAAGTTTAAA TCCCAAAACA 
TGGGGAAGAT TAAGTACACA GTCTAACAGT AATAACATTG AACCAGCAAG AACAGCAGGT 
GTAAGTGGTC TTGCAAGAGC TGCTTCTAAG GATACAATTT CCAATAACAG AGAAAAAATT 
AAGGGTTGGA TTAAGGAGCA AGCCCATAAA TTTGTAGACC GTTATTTTAG TTCGGAAAAC 
ATGGATGGAA GCAATCCTGC ACTAAATGTA TTACAGAGAC TTTGCACTGC AACTGAACAA 
CTTAACCTCC AGGTGGATGG TGGAACAGAG TGCCTTGTAG AAATCCGTAG CATTGTCTCG 
GAGTCTGACG TCTCCTCATT TGAAATCCAG CATAGTGGAT TTGTGAAACA ACTGCTGCTT 
TATTTGACAT CTAAAAGTGA GAAAGATGCT GTAAGCAGGG ATATCAGATT GAAAAGATTT 
CTTCATGTAT TTTTCTCCTC TCCACTTCCT GGAGAAGAAC CCCTTGGAAG ATTAGAGCCA 
TTAGAAAATG CACCTTTGTT GGCATTAGTC CATAAAATGA ACAATTGCCT CAGTCAGATG 
GAGCAGTTTC CAGTGAAAGT GCATGACTTC CCCAGTGGAA ATGGAACAGG GAGCAGTTTT 
TCTCTTAACA GAGGATCCCA AGCTTTAAAA TTCTTCAATA CGCATCAGTT AAAATGCCAG 
CTGCAAAGAC ATCCAGACTG TGCTAATGTG AAACAGTGGA AGGGTGGACC TGTGAAGATT 
GATCCTCTGG CTTTGGTTCA AGCCATTGAA AGATATCTTG TTGTTAGAGG TTATGGAAGA 
GTTAGAGAAG ATGATGAGGA TAGTGATGAT GATGGGTCAG ATGAAGAAAT AGATGAATCC 
TTGGCTGCTC AATTCTTAAA TTCAGGGAAT GTGAGACATA GATTGCAGTT TTACATTGGG 
GATCACTTGC TACCATATAA TATGACTGTA TATCAAGCAG TTAAGCTGTA CAGTTTGCAA 
GCTGAGGAGG AGAGGGAATC GACTGATGAT GAAAGCAATC CATTAGGAAG AGCTGGGATT 
TGGACAAAAA CACATACCAT TTGGTACAAA CCTGTACGGG AGGATGAAGA TGGCAACAAA 
GACTGTGTTG GTGGTAAAAG AGGAAGAGCT CAAACTGCTC CCACAAAAAC CTCACCTAGG 
AATTCTAAAA AGCATGATGA GTTGTGGCAC GATGGTGTAT GCCCTTCAGT ATTGAATCCT 
TTAGAAGTTT ACCTCATATC TACTCCTCCT GAAAACATAA CCTTTGAAGA TCCTTCATTA 
GATGTTATTC TTCTTTTGAG AGTTTTACAT GCTATCAGTC GATACTGGTA TTATTTGTAT 
GATAATGCAA TATGCAAGGA GATTATTCCA ACTTCGGAAT TTATCAATAG TAAACTGACA 
GCAAAAGCAA ACAGACAGCT TCAAGATCCA CTGGTAATTA TGACAGGAAA CATACCGACT 
TGGCTGACAG AGCTTGGAAA AACATGCCCA TTTTTCTTTC CATTTGATAC CCGGCAAATG 
CTGTTTTATG TAACTGCTTT TGATCGTGAT CGAGCCATGC AAAGACTACT GGATACTAAT 
CCAGAAATCA ATCAATCAGA TTCTCAGGAT AGCAGAGTGG CACCACGACT GGACAGGAAA 
AAACGCACTG TGAACAGAGA TGAGCTGTTG AAACAGGCAG AATCTGTGAT GCAGGATCTA 
GGCAGTTCAA GAGCCATGTT GGAAATCCAG TATGAGAATG AAGTTGGCAC AGGCCTGGGC 
CCTACACTAG AGTTCTATGC ACTTGTATCT CAGGAACTAC AGAGAGCAGA CTTGGGCCTT 
TGGAGGGGAG AAGAAGTAAC TTTAGCCAAT CCAAAAGGAA GCCAAGAAGG TACCAAGTAT 
ATTCATAATC TCCAAGGCCT TTTTGCACTT CCTTTTGGTA GAACAGCCAA GCCTGCTCAC 
ATTGCAAAAG TTAAAATGAA GTTCCGCTTT CTGGGAAAAC TAATGGCTAA GGCAATCATG 
GATTTTAGAC TGGTGGATCT TCCTCTTGGA CTTCCTTTTT ATAAATGGAT GTTACGCCAG 
GAAACTTCTT TGACGTCACA TGACTTGTTC AGTATTGATC CAGTAGTAGC CAAATCAATA 
TATCACCTTG AAGATATTGT AAGACAAAAG AAAAGACTTG AGCAGGACAA AACACAGACC 
AAAGAAAGTC TACAGTATGC ATTGGAAACC CTGACTATGA ATGGCTGCTC AGTGGAGGAT 
CTAGGGCTGG ATTTCACACT TCCTGGATTT CCTAACATCG AGCTGAAAAA AGGGGGAAAA 
GATACACCAG TCACCATCCA CAACTTGGAG GAGTACCTCA GATTGGTTAT ATTCTGGGCA 
CTTAACGAAG GTGTTGCCAG ACAGTTTGAT TCTTTCAGAG ATGGATTTGA ATCAGTCTTC 
CCCCTCAGTC ATCTTCAATA CTTTTATCCT GAGGAGTTGG AGCAACTCTT ATGTGGCAGC 
AAAACAGACA CATGGGATGC CAAGACCTTA ATGGAATGTT GCAGGCCAGA TCACGGTTAT 
ACACATGACA GTCGAGCAGT GAAGTATCTC TTTGAAATTC TCAGTAGTTT TGATAGTGAG 
CAGCAGAGAC TGTTTCTTCA GTTTGTGACC GGTAGTCCCA GGCTGCCAGT AGGAGGCTTT 
CGAAGTTTGA ATCCTCCATT GACAATTGTA CGGAAGACAT TTGAGTCTAC AGAGAATCCA 
GATGACTTCT TGCCCTCAGT AATGACTTGT GTGAACTATC TCAAATTGCC GGACTATTCA 
ACTATTGAGA TAATGCGTGA AAAACTCTTG ATAGCTGCAA GAGAAGGGCA GCAGTCATTC 
CATCTTTCCT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Gene Symbol	TRIP12
Entrez Gene ID	424785
Full Name	thyroid hormone receptor interactor 12
General protein information	Preferred Names thyroid hormone receptor interactor 12 Names probable E3 ubiquitin-protein ligase TRIP12 E3 ubiquitin-protein ligase TRIP12
Gene Type	protein-coding
Organism	Gallus gallus(chicken)

Anopheles gambiae (African malaria mosquito)	AgaP_AGAP001296	XP_321851.5
	TRIP12	XP_002799130.1
Mus musculus (house mouse)	Trip12	NP_598736.4
Xenopus tropicalis (tropical clawed frog)	trip12	NP_001136384.1
Homo sapiens (human)	TRIP12	NP_004229.1
Canis lupus familiaris (dog)	TRIP12	XP_005635860.1
Gallus gallus (chicken)	TRIP12	XP_004937121.1
Pan troglodytes (chimpanzee)	TRIP12	XP_001138950.1
Bos taurus (cattle)	TRIP12	NP_001178132.1
Arabidopsis thaliana (thale cress)	KAK	NP_849567.2
Rattus norvegicus (Norway rat)	Trip12	NP_001026829.1
Danio rerio (zebrafish)	trip12	XP_005159284.1
Oryza sativa (rice)	Os02g0101700	NP_001045591.1

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
30% OFF

TRIP12 cDNA ORF clone, Gallus gallus(chicken)

Gene

Clones

gRNAs

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

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Target ORF information:

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Target ORF information:

Target ORF information:

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Target ORF information:

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Target ORF information:

Target ORF information:

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Target ORF information:

Target ORF information:

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Target ORF information:

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Target ORF information:

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Target ORF information:

Target ORF information:

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Target ORF information:

Target ORF information:

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Target ORF information:

Target ORF information:

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Target ORF information:

Target ORF information:

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

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Target ORF information:

Target ORF information:

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Target ORF information:

Target ORF information:

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