THY1 cDNA ORF clone, Canis lupus familiaris(dog)

Gene
Clones
gRNAs

Summary

Gene Symbol	THY1
Entrez Gene ID	489365
Full Name	Thy-1 cell surface antigen
General protein information	Preferred Names Thy-1 cell surface antigen Names thy-1 membrane glycoprotein
Gene Type	protein-coding
Organism	Canis lupus familiaris(dog)

mRNA and Protein(s)

mRNA	Protein	Name
NM_001287129.1	NP_001274058.1	thy-1 membrane glycoprotein precursor

Pathways from BioSystems

Homologies

Homo sapiens (human)	THY1	NP_006279.2
Xenopus tropicalis (tropical clawed frog)	thy1	XP_004916102.1
Canis lupus familiaris (dog)	THY1	XP_849699.1
Mus musculus (house mouse)	Thy1	NP_033408.1
Gallus gallus (chicken)	THY1	NP_989712.1
Pan troglodytes (chimpanzee)	THY1	XP_003313415.1
Bos taurus (cattle)	THY1	NP_001029937.1
	THY1	NP_001036103.1
Rattus norvegicus (Norway rat)	Thy1	NP_036805.1

Gene Ontology
Bibliography

Related articles in PubMed

Synovial fluid progenitors expressing CD90+ from normal but not osteoarthritic joints undergo chondrogenic differentiation without micro-mass culture.
Krawetz RJ, Wu YE, Martin L, Rattner JB, Matyas JR, Hart DA
PloS one7(8)e43616(2012)

Construction and characterization of an eightfold redundant dog genomic bacterial artificial chromosome library.
Li R, Mignot E, Faraco J, Kadotani H, Cantanese J, Zhao B, Lin X, Hinton L, Ostrander EA, Patterson DF, de Jong PJ
Genomics58(1)9-17(1999 May)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

an increased chondrogenic potential within the CD90+ fraction of human and canine synovial fluid mesenchymal progenitor cells
Title: Synovial fluid progenitors expressing CD90+ from normal but not osteoarthritic joints undergo chondrogenic differentiation without micro-mass culture.

The following THY1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the THY1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OCe00470	NM_001287129.1 Latest version!	Canis lupus familiaris Thy-1 cell surface antigen (THY1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$104.30 $149.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

THY1 ( NM_001287129.1 ) cDNA ORF clone, Canis lupus familiaris(dog) -> NP_001274058.1 Canis lupus familiaris Thy-1 cell surface antigen (THY1), mRNA.

Price & Availability↑

CloneID	OCe00470
Clone ID Related Accession (Same CDS sequence)	NM_001287129.1
Accession Version	NM_001287129.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 486bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-10-22
Organism	Canis lupus familiaris(dog)
Product	thy-1 membrane glycoprotein precursor
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from GR899539.1, DN442623.1 and AAEX03003436.1. On Nov 27, 2013 this sequence version replaced XM_844606.3. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. ##Evidence-Data-START## Transcript exon combination :: GR899539.1, DN395060.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA103936001, SAMEA103936002 [ECO:0000348] ##Evidence-Data-END##

ATGAACCCCG CCATCGGCTT CGCTCTTTTG CTGACAGTCT TGCAGGTAGC CCGTGGACAA 
AAGGTGACCA GCCTGACAGC CTGCCTGGTG GACCAGAGCC TTCGTCTGGA CTGCCGCCAC 
GAGAATGCTA CCACCTTGCC CATCCAGTAT GAGTTCAGCA TGACCCGGGA GAAAAAGCAG 
CACGTGATCT ATGGCACTGT GGGGGTACCA GAGCACTCAT ACCGCTCCCG AACCAATTTC 
ACCAGCAAGT ACAACATCAA GGTCCTCTAC TTATCCGGCT TCACCACCAA GGACGAGGGG 
ACATACACAT GTGAACTCCG GCTCTCTGGC CAGCCTCCCA TCACCTCCAG CAAGAACGTC 
ACTGTGCTCA GAGACAAACT GGTCAAGTGT GAGGGCATAA GCCTGCTGGC CCAGAACACC 
TCATGGCTGC TGTTGCTCCT GCTCTCGCTG CCTCTCCTGC AGGCCATGGA CTTCATCTCC 
CTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001274058.1
CDS	105..590
Translation	MNPAIGFALLLTVLQVARGQKVTSLTACLVDQSLRLDCRHENATTLPIQYEFSMTREKKQHVIYGTVGVPEHSYRSRTNFTSKYNIKVLYLSGFTTKDEGTYTCELRLSGQPPITSSKNVTVLRDKLVKCEGISLLAQNTSWLLLLLLSLPLLQAMDFISL

Target ORF information:

RefSeq Version	NM_001287129.1
Organism	Canis lupus familiaris(dog)
Definition	Canis lupus familiaris Thy-1 cell surface antigen (THY1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001287129.1

ORF Insert Sequence:

ATGAACCCCG CCATCGGCTT CGCTCTTTTG CTGACAGTCT TGCAGGTAGC CCGTGGACAA 
AAGGTGACCA GCCTGACAGC CTGCCTGGTG GACCAGAGCC TTCGTCTGGA CTGCCGCCAC 
GAGAATGCTA CCACCTTGCC CATCCAGTAT GAGTTCAGCA TGACCCGGGA GAAAAAGCAG 
CACGTGATCT ATGGCACTGT GGGGGTACCA GAGCACTCAT ACCGCTCCCG AACCAATTTC 
ACCAGCAAGT ACAACATCAA GGTCCTCTAC TTATCCGGCT TCACCACCAA GGACGAGGGG 
ACATACACAT GTGAACTCCG GCTCTCTGGC CAGCCTCCCA TCACCTCCAG CAAGAACGTC 
ACTGTGCTCA GAGACAAACT GGTCAAGTGT GAGGGCATAA GCCTGCTGGC CCAGAACACC 
TCATGGCTGC TGTTGCTCCT GCTCTCGCTG CCTCTCCTGC AGGCCATGGA CTTCATCTCC 
CTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Synovial fluid progenitors expressing CD90+ from normal but not osteoarthritic joints undergo chondrogenic differentiation without micro-mass culture.
PloS one7(8)e43616(2012)
Krawetz RJ,Wu YE,Martin L,Rattner JB,Matyas JR,Hart DA

Construction and characterization of an eightfold redundant dog genomic bacterial artificial chromosome library.
Genomics58(1)9-17(1999 May)
Li R,Mignot E,Faraco J,Kadotani H,Cantanese J,Zhao B,Lin X,Hinton L,Ostrander EA,Patterson DF,de Jong PJ