TRIP12 gene cDNA ORF clone, Canis lupus familiaris(dog)

mRNA	Protein	Name
XM_005635800.3	XP_005635857.1	E3 ubiquitin-protein ligase TRIP12 isoform X1
XM_022409664.1	XP_022265372.1	E3 ubiquitin-protein ligase TRIP12 isoform X2
XM_022409672.1	XP_022265380.1	E3 ubiquitin-protein ligase TRIP12 isoform X11
XM_005635801.2	XP_005635858.1	E3 ubiquitin-protein ligase TRIP12 isoform X1
XM_005635800.2	XP_005635857.1	E3 ubiquitin-protein ligase TRIP12 isoform X1
XM_005635804.2	XP_005635861.1	E3 ubiquitin-protein ligase TRIP12 isoform X4
XM_005635801.3	XP_005635858.1	E3 ubiquitin-protein ligase TRIP12 isoform X1
XM_022409670.1	XP_022265378.1	E3 ubiquitin-protein ligase TRIP12 isoform X8
XM_005635803.2	XP_005635860.1	E3 ubiquitin-protein ligase TRIP12 isoform X3
XM_014107492.1	XP_013962967.1	E3 ubiquitin-protein ligase TRIP12 isoform X9
XM_022409663.1	XP_022265371.1	E3 ubiquitin-protein ligase TRIP12 isoform X1
XM_022409666.1	XP_022265374.1	E3 ubiquitin-protein ligase TRIP12 isoform X4
XM_022409671.1	XP_022265379.1	E3 ubiquitin-protein ligase TRIP12 isoform X9
XM_022409674.1	XP_022265382.1	E3 ubiquitin-protein ligase TRIP12 isoform X12
XM_022409676.1	XP_022265384.1	E3 ubiquitin-protein ligase TRIP12 isoform X14
XM_005635802.2	XP_005635859.1	E3 ubiquitin-protein ligase TRIP12 isoform X2
XM_005635809.2	XP_005635866.1	E3 ubiquitin-protein ligase TRIP12 isoform X8
XM_005635807.1	XP_005635864.1	E3 ubiquitin-protein ligase TRIP12 isoform X6
XM_022409668.1	XP_022265376.1	E3 ubiquitin-protein ligase TRIP12 isoform X6
XM_005635808.3	XP_005635865.1	E3 ubiquitin-protein ligase TRIP12 isoform X10
XM_005635805.2	XP_005635862.1	E3 ubiquitin-protein ligase TRIP12 isoform X5
XM_005635808.2	XP_005635865.1	E3 ubiquitin-protein ligase TRIP12 isoform X7
XM_022409665.1	XP_022265373.1	E3 ubiquitin-protein ligase TRIP12 isoform X3
XM_022409667.1	XP_022265375.1	E3 ubiquitin-protein ligase TRIP12 isoform X5
XM_022409669.1	XP_022265377.1	E3 ubiquitin-protein ligase TRIP12 isoform X7
XM_022409675.1	XP_022265383.1	E3 ubiquitin-protein ligase TRIP12 isoform X13

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OCe297067	XM_022409675.1 Latest version!	Canis lupus familiaris thyroid hormone receptor interactor 12 (TRIP12), transcript variant X15, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$1021.30 $1459.00
OCe43046	XM_014107492.1 Latest version!	Canis lupus familiaris thyroid hormone receptor interactor 12 (TRIP12), transcript variant X10, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$1021.30 $1459.00
OCe43046	XM_022409676.1 Latest version!	Canis lupus familiaris thyroid hormone receptor interactor 12 (TRIP12), transcript variant X16, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$1021.30 $1459.00
OCe25766	XM_022409669.1 Latest version!	Canis lupus familiaris thyroid hormone receptor interactor 12 (TRIP12), transcript variant X9, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1161.30 $1659.00
OCe297063	XM_022409666.1 Latest version!	Canis lupus familiaris thyroid hormone receptor interactor 12 (TRIP12), transcript variant X6, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1161.30 $1659.00
OCe297064	XM_022409670.1 Latest version!	Canis lupus familiaris thyroid hormone receptor interactor 12 (TRIP12), transcript variant X10, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1161.30 $1659.00
OCe297065	XM_022409671.1 Latest version!	Canis lupus familiaris thyroid hormone receptor interactor 12 (TRIP12), transcript variant X11, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1161.30 $1659.00
OCe297066	XM_022409674.1 Latest version!	Canis lupus familiaris thyroid hormone receptor interactor 12 (TRIP12), transcript variant X14, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1161.30 $1659.00
OCe25761	XM_005635800.3 Latest version!	Canis lupus familiaris thyroid hormone receptor interactor 12 (TRIP12), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	30	$1161.30 $1659.00
OCe25761	XM_022409663.1 Latest version!	Canis lupus familiaris thyroid hormone receptor interactor 12 (TRIP12), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	30	$1161.30 $1659.00
OCe25761	XM_005635801.3 Latest version!	Canis lupus familiaris thyroid hormone receptor interactor 12 (TRIP12), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	30	$1161.30 $1659.00
OCe25762	XM_005635802.2 Latest version!	Canis lupus familiaris thyroid hormone receptor interactor 12 (TRIP12), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	30	$1161.30 $1659.00
OCe25762	XM_022409664.1 Latest version!	Canis lupus familiaris thyroid hormone receptor interactor 12 (TRIP12), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	30	$1161.30 $1659.00
OCe25763	XM_005635803.2 Latest version!	Canis lupus familiaris thyroid hormone receptor interactor 12 (TRIP12), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	30	$1161.30 $1659.00
OCe25763	XM_022409665.1 Latest version!	Canis lupus familiaris thyroid hormone receptor interactor 12 (TRIP12), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	30	$1161.30 $1659.00
OCe25764	XM_005635804.2 Latest version!	Canis lupus familiaris thyroid hormone receptor interactor 12 (TRIP12), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	30	$1161.30 $1659.00
OCe25764	XM_022409667.1 Latest version!	Canis lupus familiaris thyroid hormone receptor interactor 12 (TRIP12), transcript variant X7, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	30	$1161.30 $1659.00
OCe25765	XM_005635805.2 Latest version!	Canis lupus familiaris thyroid hormone receptor interactor 12 (TRIP12), transcript variant X6, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	30	$1161.30 $1659.00
OCe25765	XM_022409668.1 Latest version!	Canis lupus familiaris thyroid hormone receptor interactor 12 (TRIP12), transcript variant X8, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	30	$1161.30 $1659.00
OCe25767	XM_005635807.1 Latest version!	Canis lupus familiaris thyroid hormone receptor interactor 12 (TRIP12), transcript variant X7, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	30	$1161.30 $1659.00
OCe25768	XM_005635808.3 Latest version!	Canis lupus familiaris thyroid hormone receptor interactor 12 (TRIP12), transcript variant X12, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	30	$1161.30 $1659.00
OCe25769	XM_005635809.2 Latest version!	Canis lupus familiaris thyroid hormone receptor interactor 12 (TRIP12), transcript variant X9, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	30	$1161.30 $1659.00
OCe25769	XM_022409672.1 Latest version!	Canis lupus familiaris thyroid hormone receptor interactor 12 (TRIP12), transcript variant X13, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	30	$1161.30 $1659.00
View Old Accession Versions >>
OCe25761	XM_005635800.2	Canis lupus familiaris thyroid hormone receptor interactor 12 (TRIP12), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	30	$1161.30 $1659.00
OCe25761	XM_005635801.2	Canis lupus familiaris thyroid hormone receptor interactor 12 (TRIP12), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	30	$1161.30 $1659.00
OCe25768	XM_005635808.2	Canis lupus familiaris thyroid hormone receptor interactor 12 (TRIP12), transcript variant X8, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	30	$1161.30 $1659.00
Hide Old Accession Versions >>

TRIP12 ( XM_022409675.1 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_022265383.1 Canis lupus familiaris thyroid hormone receptor interactor 12 (TRIP12), transcript variant X15, mRNA.

Price & Availability↑

CloneID	OCe297067
Clone ID Related Accession (Same CDS sequence)	XM_022409675.1
Accession Version	XM_022409675.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5982bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-09-04
Organism	Canis lupus familiaris(dog)
Product	E3 ubiquitin-protein ligase TRIP12 isoform X13
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006607.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCCAACC GGCCTAATAA CAATCCAGGG GGGTCACTGC GACGTTCACA GAGGAACACT 
GCCGGGGCCC AACCACAAGA CGACTCGATA GGAGGAAGAA GCTGCAGTTC ATCATCTGCT 
GTCATAGTTC CACAACCAGA GGATCCAGAC AGAGCCAATA CTTCAGAAAG GCAAAAAACG 
GGGCAGGTGC CTAAGAAAGA CAATTCTCGA GGAGTGAAAC GCAGTGCTAG TCCAGACTAC 
AACAGGACCA ATTCTCCTAG CTCTGCCAAA AAACCAAAAG CACTTCAGCA TACTGAATCT 
CCCTCAGAAA CAAATAAGCC ACATAGTAAG TCGAAGAAGA GACATTTAGA CCAGGAGCAA 
CAACTGAAAT CTGCACAGTC GCCATCAACA AGTAAGGCTC ACACCAGAAA GAGTGGGGCC 
ACCGGCGGCT CACGGAGTCA GAAAAGAAAG AGGACAGAGA GTTCTTGTCT AAAGAGTGGT 
TCTGTGTCTG AGTCAACTGG TGCCGAAGAG AGATCTGCAA AACCCACCAA GCTGGCTTCA 
AAATCCGCCG CCTCGGCCAA AGCTGGGTGC AGCACCATCA CTGATTCTTC CTCCGCTGCC 
TCTACTTCCT CCTCATCTTC TGCTGTCGCC TCGGCCTCCT CCACTGTACC ACCAGGGGCC 
AGGGTGAAAC AAGGAAAAGA CCAGAGCAAG GCCAGGCGTT CCCGTTCAGC ATCCAGTCCC 
AGTCCCAGAA GAAGTAGCAG GGAAAAGGAA CAGAGTAAAA CTGGTGGCTC TTCAAAATTC 
GATTGGGCTG CTCGTTTCAG CCCTAAAGTT AGCCTTCCTA AAACCAAACT GTCTCTTCCA 
GGGTCTTCTA AGTCAGAGAC ATCAAAACCT GGACCTTCTG GATTACAGGC CAAATTAGCA 
AGTTTAAGAA AATCTACCAA GAAGCGCAGT GAATCCCCAC CTGCTGAGCT CCCCAGTTTG 
AGGCGGAGCA CACGCCAAAA GACCACGGGC TCCTGTGCTA GTGCCAGTCG GCGAGGCTCT 
GGCCTGGGCA AAAGAGGAGC AGCTGAAGCT CGTCGACAGG AGAAGATGGC GGACCCTGAA 
AGCAGCCAGG AGACCGTGAA TTCTTCAGCT GCACGGACAG ACGAAACTCC CCAAGGAGCT 
GCAGCCTCTA GTTCTGTTGC AGGGGCTGTG GGCATGACCA CCTCTGGGGA GAGTGAATCC 
GATGATTCTG AGATGGGACG ACTACAAGCT TTGTTAGAGG CCAGGGGTCT TCCTCCTCAT 
CTGTTTGGCC CTCTCGGTCC TCGGATGTCA CAACTTTTCC ATAGAACAAT TGGAAGCGGA 
GCTAGCTCTA AGGCCCAGCA GCTTCTGCAA GGACTACAAG CAAGTGATGA AAGCCAGCAA 
CTCCAGGCAG TTATTGAAAT GTGTCAGTTA TTGGTTATGG GAAATGAGGA GACGCTGGGA 
GGGTTTCCGG TCAAGAGTGT CGTTCCAGCT TTGATAACAT TACTGCAGAT GGAGCATAAT 
TTCGATATTA TGAACCATGC TTGTCGAGCC TTAACATATA TGATGGAAGC ACTTCCTCGG 
TCATCTGCTG TTGTAGTAGA TGCAATTCCT GTCTTTTTAG AAAAGCTGCA AGTTATTCAG 
TGTATTGATG TCGCAGAGCA GGCCCTGACT GCCTTGGAGA TGTTATCACG CAGACACAGT 
AAAGCCATTC TACAGGCGGG TGGTTTGGCA GACTGCTTGC TGTATCTAGA ATTCTTCAGC 
ATAAATGCCC AAAGAAATGC ACTAGCAATT GCAGCCAATT GCTGCCAGAG TATCACGCCA 
GATGAATTTC ATTTTGTGGC AGATTCCCTC CCGTTGCTTA CCCAAAGGCT AACCCATCAG 
GACAAAAAGT CAGTTGAAAG CACTTGCCTT TGTTTTGCAC GTCTAGTGGA CAACTTCCAA 
CATGAGGAGA ATTTACTCCA GCAGGTTGCC TCCAAAGATC TGCTTACGAA TGTTCAACAA 
CTCTTGGTAG TGACTCCACC CATTTTAAGT TCTGGGATGT TTATAATGGT GGTTCGCATG 
TTTTCTCTGA TGTGTTCCAA CTGTCCAACT TTAGCTGTTC AACTTATGAA GCAAAACATT 
GCAGAAACAC TTCACTTTCT CCTGTGTGGT GCCTCTAATG GAAGTTGTCA GGAACAGATT 
GATCTTGTTC CACGAAGTCC ACAAGAACTG TATGAACTGA CATCTCTGAT TTGCGAACTT 
ATGCCATGTT TACCAAAAGA AGGCATTTTT GCAGTTGATA CCATGCTGAA GAAGGGAAAT 
GCGCAGAACA CAGACGGTGC GATTTGGCAG TGGCGTGATG ACCGTGGCCT TTGGCATCCC 
TATAACAGGA TTGACAGCCG GATCATTGAG CAAATCAATG AGGACACGGG AACAGCACGT 
GCCATTCAGA GAAAACCTAA CCCCTTAGCC AATACTAACA CTAGTGGATA TTCAGAGTTA 
AAGAAGGATG ATGCTCGAGC ACAGCTTATG AAAGAGGATC CGGAACTGGC TAAGTCTTTT 
ATTAAGACAT TATTTGGTGT TCTTTATGAA GTGTATAGTT CCTCAGCAGG ACCTGCGGTC 
AGACATAAGT GCCTTAGAGC AATTCTTAGG ATAATTTATT TTGCGGATGC TGAACTTCTG 
AAGGATGTCC TGAAAAATCA TGCTGTTTCA AGTCACATTG CTTCCATGCT GTCAAGCCAA 
GACCTGAAGA TAGTAGTTGG AGCACTTCAG ATGGCAGAAA TCTTAATGCA GAAGTTACCT 
GATATTTTTA GTGTTTACTT CAGAAGAGAA GGTGTAATGC ATCAAGTAAA ACACTTAGCA 
GAATCAGAGT CTTTGTTGAC AAGTCCACCA AAGGCATGTA CGAATGGGTC AGGATCCCTG 
GGATCCACGA CGTCAGTCAG CAGTGGAACA GCCACGGCCG CCACCAATGC TGCAGCTGAC 
CTGGGGTCCC CCAGCTTGCA GCACAGCAGA GATGACTCTT TAGATCTGAG CCCTCAAGGT 
CGATTAAGTG ATGTTCTAAA GAGAAAACGG CTGCCAAAAC GAGGGCCAAG AAGGCCAAAG 
TATTCACCTC CAAGAGATGA TGACAAAGTA GACAATCAAG CTAAAAGCCC TACCACTACT 
CAGTCACCTA AATCTTCTTT CCTGGCAAGC TTGAATCCAA AAACGTGGGG AAGGTTAAGC 
GCACAGTCCA ACAGCAACAA CATCGAACCA GCACGAACTG CGGGAGTCAG TGGTCTTGCC 
AGGGCCGCCT CCAAGGACAC CATATCCAAT AACAGAGAAA AAATTAAAGG TTGGATTAAG 
GAACAGGCGC ATAAATTTGT AGAGCGTTAT TTCAGTTCTG AGAACATGGA TGGAAGCAAC 
CCTGCACTGA ATGTCCTTCA GAGACTTTGT GCTGCAACTG AACAACTCAA CCTCCAGGTG 
GATGGTGGAG CTGAGTGCCT TGTAGAAATC CGTAGCATAG TCTCAGAGTC TGATGTTTCA 
TCATTTGAAA TCCAACATAG TGGATTTGTG AAGCAGCTGT TACTTTATTT GACATCTAAA 
AGTGAAAAGG ATGCTGTGAG CCGAGAGATC AGATTAAAGA GATTCCTTCA CGTATTTTTT 
TCTTCACCAC TTCCTGGAGA AGAGCCCATT GGAAGAGTAG AACCAGTGGG TAATGCGCCT 
TTGTTGGCAT TAGTTCACAA GATGAACAAC TGCCTCAGCC AGATGGAACA GTTTCCAGTC 
AAAGTACATG ACTTCCCCAG TGGGAACGGG ACAGGAGGCA GAGGATCACA GGCTTTAAAA 
TTTTTCAACA CACACCAGTT AAAATGCCAG TTACAAAGAC ATCCAGACTG TGCAAATGTG 
AAGCAGTGGA AGGGCGGGCC GGTCAAGATT GATCCCCTGG CTTTGGTACA AGCTATTGAG 
AGATACCTTG TAGTTAGAGG GTATGGAAGA GTAAGAGAAG ATGATGAAGA CAGTGATGAT 
GATGGCTCTG ATGAGGAAAT AGACGAGTCT CTGGCCGCTC AGTTCTTAAA TTCCGGAAAC 
GTAAGGCACA GGCTACAGTT TTATATTGGA GAACATTTGC TACCATATAA CATGACTGTA 
TATCAGGCAG TCCGGCAGTT CAGTATACAA GCTGAGGATG AGCGGGAGTC CACAGATGAT 
GAGAGCAACC CTCTGGGAAG AGCTGGTATT TGGACAAAGA CTCATACAAT ATGGTACAAA 
CCTGTGAGAG AGGATGAAGA AAGTAATAAA GATTGTGTTG GTGGTAAAAG AGGAAGAGCC 
CAAACGGCTC CAACAAAAAC ATCCCCTAGA AATGCAAAAA AGCATGATGA GTTATGGCAT 
GATGGAGTAT GCCCATCAGT ATCCAATCCT TTAGAAGTTT ACCTCATCTC CTCGCCACCT 
GAGAATATAA CTTTTGAAGA CCCGTCCTTA GATGTGATTC TTCTTCTAAG AGTTTTACAT 
GCCATCAGCC GATACTGGTA TTACTTGTAT GATAATGCAA TGTGCAAGGA GATTATTCCA 
ACTAGTGAAT TTATTAACAG TAAGTTGACA GCAAAAGCAA ATAGGCAGCT TCAAGATCCT 
TTAGTAATCA TGACAGGAAA TATCCCAACG TGGCTTACTG AACTAGGAAA AACCTGCCCA 
TTTTTCTTCC CTTTTGATAC CCGGCAAATG CTTTTTTACG TAACTGCATT TGATCGGGAC 
CGAGCAATGC AAAGATTACT TGATACCAAC CCAGAAATCA ACCAGTCGGA TTCTCAAGAT 
AGCAGAGTTG CACCTAGATT GGATAGAAAA AAACGTACTG TGAACAGAGA GGAGCTGCTG 
AAACAAGCGG AGTCTGTAAT GCAGGATCTC GGTAGCTCAC GGGCCATGTT AGAAATCCAG 
TATGAAAATG AGGTTGGTAC AGGTCTTGGG CCTACACTGG AGTTTTATGC ACTTGTATCT 
CAGGAACTTC AGAGAGCTGA CTTGGGTCTC TGGAGAGGTG AAGAAGTGAC TCTCAGCAAT 
CCAAAAGGAA GCCAAGAAGG GACCAAGTAT ATTCAAAACC TCCAGGGCCT GTTTGCACTT 
CCCTTTGGTA GGACAGCTAA GCCAGCCCAT ATCGCAAAGG TTAAAATGAA GTTTCGCTTC 
TTAGGAAAAT TAATGGCCAA GGCTATCATG GATTTCAGAT TGGTGGACCT TCCCCTTGGT 
TTACCCTTTT ATAAGTGGAT GCTAAGGCAA GAAACTTCAC TGACGTCGCA TGATTTGTTT 
GACATTGACC CAGTTGTAGC CAGATCAGTG TATCACCTAG AAGACATTGT CAGACAGAAG 
AAAAGACTTG AGCAAGATAA ATCCCAGACC AAAGAGAGTC TACAATATGC ATTAGAAACC 
CTGACTATGA ATGGCTGCTC AGTTGAAGAT CTAGGATTGG ATTTCACGCT GCCAGGGTTT 
CCCAACATTG AACTGAAGAA AGGAGGAAAG GATATACCAG TTACCATCCA CAATTTAGAG 
GAGTATCTAA GACTGGTTAT ATTCTGGGCA CTAAATGAAG GCGTTTCCAG GCAATTTGAT 
TCCTTCAGAG ATGGATTTGA ATCTGTCTTC CCACTTAGTC ATCTTCAGTA CTTCTACCCG 
GAAGAACTGG ATCAGCTCTT GTGCGGCAGT AAAGCAGACA CGTGGGATGC GAAGACACTG 
ATGGAGTGCT GCAGGCCGGA CCACGGTTAT ACTCATGACA GTCGGGCTGT GAAGTTCTTG 
TTTGAGATTC TCAGTAGTTT TGATAATGAG CAGCAGAGGT TGTTTCTCCA GTTTGTGACA 
GGTAGCCCAA GATTGCCTGT TGGAGGCTTC CGGAGTCTGA ATCCACCTCT GACAATTGTC 
CGGAAGACCT TCGAGTCCAC GGAGAACCCC GACGACTTCT TGCCCTCTGT AATGACTTGT 
GTGAACTACC TTAAGTTGCC GGACTACTCG AGCATTGACA TCATGCGTGA GAAACTGTTG 
ATTGCAGCGA GAGAAGGGCA GCAGTCATTC CATCTTTCCT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_022265383.1
CDS	1..5982
Translation	MSNRPNNNPGGSLRRSQRNTAGAQPQDDSIGGRSCSSSSAVIVPQPEDPDRANTSERQKTGQVPKKDNSRGVKRSASPDYNRTNSPSSAKKPKALQHTESPSETNKPHSKSKKRHLDQEQQLKSAQSPSTSKAHTRKSGATGGSRSQKRKRTESSCLKSGSVSESTGAEERSAKPTKLASKSAASAKAGCSTITDSSSAASTSSSSSAVASASSTVPPGARVKQGKDQSKARRSRSASSPSPRRSSREKEQSKTGGSSKFDWAARFSPKVSLPKTKLSLPGSSKSETSKPGPSGLQAKLASLRKSTKKRSESPPAELPSLRRSTRQKTTGSCASASRRGSGLGKRGAAEARRQEKMADPESSQETVNSSAARTDETPQGAAASSSVAGAVGMTTSGESESDDSEMGRLQALLEARGLPPHLFGPLGPRMSQLFHRTIGSGASSKAQQLLQGLQASDESQQLQAVIEMCQLLVMGNEETLGGFPVKSVVPALITLLQMEHNFDIMNHACRALTYMMEALPRSSAVVVDAIPVFLEKLQVIQCIDVAEQALTALEMLSRRHSKAILQAGGLADCLLYLEFFSINAQRNALAIAANCCQSITPDEFHFVADSLPLLTQRLTHQDKKSVESTCLCFARLVDNFQHEENLLQQVASKDLLTNVQQLLVVTPPILSSGMFIMVVRMFSLMCSNCPTLAVQLMKQNIAETLHFLLCGASNGSCQEQIDLVPRSPQELYELTSLICELMPCLPKEGIFAVDTMLKKGNAQNTDGAIWQWRDDRGLWHPYNRIDSRIIEQINEDTGTARAIQRKPNPLANTNTSGYSELKKDDARAQLMKEDPELAKSFIKTLFGVLYEVYSSSAGPAVRHKCLRAILRIIYFADAELLKDVLKNHAVSSHIASMLSSQDLKIVVGALQMAEILMQKLPDIFSVYFRREGVMHQVKHLAESESLLTSPPKACTNGSGSLGSTTSVSSGTATAATNAAADLGSPSLQHSRDDSLDLSPQGRLSDVLKRKRLPKRGPRRPKYSPPRDDDKVDNQAKSPTTTQSPKSSFLASLNPKTWGRLSAQSNSNNIEPARTAGVSGLARAASKDTISNNREKIKGWIKEQAHKFVERYFSSENMDGSNPALNVLQRLCAATEQLNLQVDGGAECLVEIRSIVSESDVSSFEIQHSGFVKQLLLYLTSKSEKDAVSREIRLKRFLHVFFSSPLPGEEPIGRVEPVGNAPLLALVHKMNNCLSQMEQFPVKVHDFPSGNGTGGRGSQALKFFNTHQLKCQLQRHPDCANVKQWKGGPVKIDPLALVQAIERYLVVRGYGRVREDDEDSDDDGSDEEIDESLAAQFLNSGNVRHRLQFYIGEHLLPYNMTVYQAVRQFSIQAEDERESTDDESNPLGRAGIWTKTHTIWYKPVREDEESNKDCVGGKRGRAQTAPTKTSPRNAKKHDELWHDGVCPSVSNPLEVYLISSPPENITFEDPSLDVILLLRVLHAISRYWYYLYDNAMCKEIIPTSEFINSKLTAKANRQLQDPLVIMTGNIPTWLTELGKTCPFFFPFDTRQMLFYVTAFDRDRAMQRLLDTNPEINQSDSQDSRVAPRLDRKKRTVNREELLKQAESVMQDLGSSRAMLEIQYENEVGTGLGPTLEFYALVSQELQRADLGLWRGEEVTLSNPKGSQEGTKYIQNLQGLFALPFGRTAKPAHIAKVKMKFRFLGKLMAKAIMDFRLVDLPLGLPFYKWMLRQETSLTSHDLFDIDPVVARSVYHLEDIVRQKKRLEQDKSQTKESLQYALETLTMNGCSVEDLGLDFTLPGFPNIELKKGGKDIPVTIHNLEEYLRLVIFWALNEGVSRQFDSFRDGFESVFPLSHLQYFYPEELDQLLCGSKADTWDAKTLMECCRPDHGYTHDSRAVKFLFEILSSFDNEQQRLFLQFVTGSPRLPVGGFRSLNPPLTIVRKTFESTENPDDFLPSVMTCVNYLKLPDYSSIDIMREKLLIAAREGQQSFHLS

Target ORF information:

RefSeq Version	XM_022409675.1
Organism	Canis lupus familiaris(dog)
Definition	Canis lupus familiaris thyroid hormone receptor interactor 12 (TRIP12), transcript variant X15, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_022409675.1

ORF Insert Sequence:

ATGTCCAACC GGCCTAATAA CAATCCAGGG GGGTCACTGC GACGTTCACA GAGGAACACT 
GCCGGGGCCC AACCACAAGA CGACTCGATA GGAGGAAGAA GCTGCAGTTC ATCATCTGCT 
GTCATAGTTC CACAACCAGA GGATCCAGAC AGAGCCAATA CTTCAGAAAG GCAAAAAACG 
GGGCAGGTGC CTAAGAAAGA CAATTCTCGA GGAGTGAAAC GCAGTGCTAG TCCAGACTAC 
AACAGGACCA ATTCTCCTAG CTCTGCCAAA AAACCAAAAG CACTTCAGCA TACTGAATCT 
CCCTCAGAAA CAAATAAGCC ACATAGTAAG TCGAAGAAGA GACATTTAGA CCAGGAGCAA 
CAACTGAAAT CTGCACAGTC GCCATCAACA AGTAAGGCTC ACACCAGAAA GAGTGGGGCC 
ACCGGCGGCT CACGGAGTCA GAAAAGAAAG AGGACAGAGA GTTCTTGTCT AAAGAGTGGT 
TCTGTGTCTG AGTCAACTGG TGCCGAAGAG AGATCTGCAA AACCCACCAA GCTGGCTTCA 
AAATCCGCCG CCTCGGCCAA AGCTGGGTGC AGCACCATCA CTGATTCTTC CTCCGCTGCC 
TCTACTTCCT CCTCATCTTC TGCTGTCGCC TCGGCCTCCT CCACTGTACC ACCAGGGGCC 
AGGGTGAAAC AAGGAAAAGA CCAGAGCAAG GCCAGGCGTT CCCGTTCAGC ATCCAGTCCC 
AGTCCCAGAA GAAGTAGCAG GGAAAAGGAA CAGAGTAAAA CTGGTGGCTC TTCAAAATTC 
GATTGGGCTG CTCGTTTCAG CCCTAAAGTT AGCCTTCCTA AAACCAAACT GTCTCTTCCA 
GGGTCTTCTA AGTCAGAGAC ATCAAAACCT GGACCTTCTG GATTACAGGC CAAATTAGCA 
AGTTTAAGAA AATCTACCAA GAAGCGCAGT GAATCCCCAC CTGCTGAGCT CCCCAGTTTG 
AGGCGGAGCA CACGCCAAAA GACCACGGGC TCCTGTGCTA GTGCCAGTCG GCGAGGCTCT 
GGCCTGGGCA AAAGAGGAGC AGCTGAAGCT CGTCGACAGG AGAAGATGGC GGACCCTGAA 
AGCAGCCAGG AGACCGTGAA TTCTTCAGCT GCACGGACAG ACGAAACTCC CCAAGGAGCT 
GCAGCCTCTA GTTCTGTTGC AGGGGCTGTG GGCATGACCA CCTCTGGGGA GAGTGAATCC 
GATGATTCTG AGATGGGACG ACTACAAGCT TTGTTAGAGG CCAGGGGTCT TCCTCCTCAT 
CTGTTTGGCC CTCTCGGTCC TCGGATGTCA CAACTTTTCC ATAGAACAAT TGGAAGCGGA 
GCTAGCTCTA AGGCCCAGCA GCTTCTGCAA GGACTACAAG CAAGTGATGA AAGCCAGCAA 
CTCCAGGCAG TTATTGAAAT GTGTCAGTTA TTGGTTATGG GAAATGAGGA GACGCTGGGA 
GGGTTTCCGG TCAAGAGTGT CGTTCCAGCT TTGATAACAT TACTGCAGAT GGAGCATAAT 
TTCGATATTA TGAACCATGC TTGTCGAGCC TTAACATATA TGATGGAAGC ACTTCCTCGG 
TCATCTGCTG TTGTAGTAGA TGCAATTCCT GTCTTTTTAG AAAAGCTGCA AGTTATTCAG 
TGTATTGATG TCGCAGAGCA GGCCCTGACT GCCTTGGAGA TGTTATCACG CAGACACAGT 
AAAGCCATTC TACAGGCGGG TGGTTTGGCA GACTGCTTGC TGTATCTAGA ATTCTTCAGC 
ATAAATGCCC AAAGAAATGC ACTAGCAATT GCAGCCAATT GCTGCCAGAG TATCACGCCA 
GATGAATTTC ATTTTGTGGC AGATTCCCTC CCGTTGCTTA CCCAAAGGCT AACCCATCAG 
GACAAAAAGT CAGTTGAAAG CACTTGCCTT TGTTTTGCAC GTCTAGTGGA CAACTTCCAA 
CATGAGGAGA ATTTACTCCA GCAGGTTGCC TCCAAAGATC TGCTTACGAA TGTTCAACAA 
CTCTTGGTAG TGACTCCACC CATTTTAAGT TCTGGGATGT TTATAATGGT GGTTCGCATG 
TTTTCTCTGA TGTGTTCCAA CTGTCCAACT TTAGCTGTTC AACTTATGAA GCAAAACATT 
GCAGAAACAC TTCACTTTCT CCTGTGTGGT GCCTCTAATG GAAGTTGTCA GGAACAGATT 
GATCTTGTTC CACGAAGTCC ACAAGAACTG TATGAACTGA CATCTCTGAT TTGCGAACTT 
ATGCCATGTT TACCAAAAGA AGGCATTTTT GCAGTTGATA CCATGCTGAA GAAGGGAAAT 
GCGCAGAACA CAGACGGTGC GATTTGGCAG TGGCGTGATG ACCGTGGCCT TTGGCATCCC 
TATAACAGGA TTGACAGCCG GATCATTGAG CAAATCAATG AGGACACGGG AACAGCACGT 
GCCATTCAGA GAAAACCTAA CCCCTTAGCC AATACTAACA CTAGTGGATA TTCAGAGTTA 
AAGAAGGATG ATGCTCGAGC ACAGCTTATG AAAGAGGATC CGGAACTGGC TAAGTCTTTT 
ATTAAGACAT TATTTGGTGT TCTTTATGAA GTGTATAGTT CCTCAGCAGG ACCTGCGGTC 
AGACATAAGT GCCTTAGAGC AATTCTTAGG ATAATTTATT TTGCGGATGC TGAACTTCTG 
AAGGATGTCC TGAAAAATCA TGCTGTTTCA AGTCACATTG CTTCCATGCT GTCAAGCCAA 
GACCTGAAGA TAGTAGTTGG AGCACTTCAG ATGGCAGAAA TCTTAATGCA GAAGTTACCT 
GATATTTTTA GTGTTTACTT CAGAAGAGAA GGTGTAATGC ATCAAGTAAA ACACTTAGCA 
GAATCAGAGT CTTTGTTGAC AAGTCCACCA AAGGCATGTA CGAATGGGTC AGGATCCCTG 
GGATCCACGA CGTCAGTCAG CAGTGGAACA GCCACGGCCG CCACCAATGC TGCAGCTGAC 
CTGGGGTCCC CCAGCTTGCA GCACAGCAGA GATGACTCTT TAGATCTGAG CCCTCAAGGT 
CGATTAAGTG ATGTTCTAAA GAGAAAACGG CTGCCAAAAC GAGGGCCAAG AAGGCCAAAG 
TATTCACCTC CAAGAGATGA TGACAAAGTA GACAATCAAG CTAAAAGCCC TACCACTACT 
CAGTCACCTA AATCTTCTTT CCTGGCAAGC TTGAATCCAA AAACGTGGGG AAGGTTAAGC 
GCACAGTCCA ACAGCAACAA CATCGAACCA GCACGAACTG CGGGAGTCAG TGGTCTTGCC 
AGGGCCGCCT CCAAGGACAC CATATCCAAT AACAGAGAAA AAATTAAAGG TTGGATTAAG 
GAACAGGCGC ATAAATTTGT AGAGCGTTAT TTCAGTTCTG AGAACATGGA TGGAAGCAAC 
CCTGCACTGA ATGTCCTTCA GAGACTTTGT GCTGCAACTG AACAACTCAA CCTCCAGGTG 
GATGGTGGAG CTGAGTGCCT TGTAGAAATC CGTAGCATAG TCTCAGAGTC TGATGTTTCA 
TCATTTGAAA TCCAACATAG TGGATTTGTG AAGCAGCTGT TACTTTATTT GACATCTAAA 
AGTGAAAAGG ATGCTGTGAG CCGAGAGATC AGATTAAAGA GATTCCTTCA CGTATTTTTT 
TCTTCACCAC TTCCTGGAGA AGAGCCCATT GGAAGAGTAG AACCAGTGGG TAATGCGCCT 
TTGTTGGCAT TAGTTCACAA GATGAACAAC TGCCTCAGCC AGATGGAACA GTTTCCAGTC 
AAAGTACATG ACTTCCCCAG TGGGAACGGG ACAGGAGGCA GAGGATCACA GGCTTTAAAA 
TTTTTCAACA CACACCAGTT AAAATGCCAG TTACAAAGAC ATCCAGACTG TGCAAATGTG 
AAGCAGTGGA AGGGCGGGCC GGTCAAGATT GATCCCCTGG CTTTGGTACA AGCTATTGAG 
AGATACCTTG TAGTTAGAGG GTATGGAAGA GTAAGAGAAG ATGATGAAGA CAGTGATGAT 
GATGGCTCTG ATGAGGAAAT AGACGAGTCT CTGGCCGCTC AGTTCTTAAA TTCCGGAAAC 
GTAAGGCACA GGCTACAGTT TTATATTGGA GAACATTTGC TACCATATAA CATGACTGTA 
TATCAGGCAG TCCGGCAGTT CAGTATACAA GCTGAGGATG AGCGGGAGTC CACAGATGAT 
GAGAGCAACC CTCTGGGAAG AGCTGGTATT TGGACAAAGA CTCATACAAT ATGGTACAAA 
CCTGTGAGAG AGGATGAAGA AAGTAATAAA GATTGTGTTG GTGGTAAAAG AGGAAGAGCC 
CAAACGGCTC CAACAAAAAC ATCCCCTAGA AATGCAAAAA AGCATGATGA GTTATGGCAT 
GATGGAGTAT GCCCATCAGT ATCCAATCCT TTAGAAGTTT ACCTCATCTC CTCGCCACCT 
GAGAATATAA CTTTTGAAGA CCCGTCCTTA GATGTGATTC TTCTTCTAAG AGTTTTACAT 
GCCATCAGCC GATACTGGTA TTACTTGTAT GATAATGCAA TGTGCAAGGA GATTATTCCA 
ACTAGTGAAT TTATTAACAG TAAGTTGACA GCAAAAGCAA ATAGGCAGCT TCAAGATCCT 
TTAGTAATCA TGACAGGAAA TATCCCAACG TGGCTTACTG AACTAGGAAA AACCTGCCCA 
TTTTTCTTCC CTTTTGATAC CCGGCAAATG CTTTTTTACG TAACTGCATT TGATCGGGAC 
CGAGCAATGC AAAGATTACT TGATACCAAC CCAGAAATCA ACCAGTCGGA TTCTCAAGAT 
AGCAGAGTTG CACCTAGATT GGATAGAAAA AAACGTACTG TGAACAGAGA GGAGCTGCTG 
AAACAAGCGG AGTCTGTAAT GCAGGATCTC GGTAGCTCAC GGGCCATGTT AGAAATCCAG 
TATGAAAATG AGGTTGGTAC AGGTCTTGGG CCTACACTGG AGTTTTATGC ACTTGTATCT 
CAGGAACTTC AGAGAGCTGA CTTGGGTCTC TGGAGAGGTG AAGAAGTGAC TCTCAGCAAT 
CCAAAAGGAA GCCAAGAAGG GACCAAGTAT ATTCAAAACC TCCAGGGCCT GTTTGCACTT 
CCCTTTGGTA GGACAGCTAA GCCAGCCCAT ATCGCAAAGG TTAAAATGAA GTTTCGCTTC 
TTAGGAAAAT TAATGGCCAA GGCTATCATG GATTTCAGAT TGGTGGACCT TCCCCTTGGT 
TTACCCTTTT ATAAGTGGAT GCTAAGGCAA GAAACTTCAC TGACGTCGCA TGATTTGTTT 
GACATTGACC CAGTTGTAGC CAGATCAGTG TATCACCTAG AAGACATTGT CAGACAGAAG 
AAAAGACTTG AGCAAGATAA ATCCCAGACC AAAGAGAGTC TACAATATGC ATTAGAAACC 
CTGACTATGA ATGGCTGCTC AGTTGAAGAT CTAGGATTGG ATTTCACGCT GCCAGGGTTT 
CCCAACATTG AACTGAAGAA AGGAGGAAAG GATATACCAG TTACCATCCA CAATTTAGAG 
GAGTATCTAA GACTGGTTAT ATTCTGGGCA CTAAATGAAG GCGTTTCCAG GCAATTTGAT 
TCCTTCAGAG ATGGATTTGA ATCTGTCTTC CCACTTAGTC ATCTTCAGTA CTTCTACCCG 
GAAGAACTGG ATCAGCTCTT GTGCGGCAGT AAAGCAGACA CGTGGGATGC GAAGACACTG 
ATGGAGTGCT GCAGGCCGGA CCACGGTTAT ACTCATGACA GTCGGGCTGT GAAGTTCTTG 
TTTGAGATTC TCAGTAGTTT TGATAATGAG CAGCAGAGGT TGTTTCTCCA GTTTGTGACA 
GGTAGCCCAA GATTGCCTGT TGGAGGCTTC CGGAGTCTGA ATCCACCTCT GACAATTGTC 
CGGAAGACCT TCGAGTCCAC GGAGAACCCC GACGACTTCT TGCCCTCTGT AATGACTTGT 
GTGAACTACC TTAAGTTGCC GGACTACTCG AGCATTGACA TCATGCGTGA GAAACTGTTG 
ATTGCAGCGA GAGAAGGGCA GCAGTCATTC CATCTTTCCT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TRIP12 ( XM_014107492.1 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_013962967.1 Canis lupus familiaris thyroid hormone receptor interactor 12 (TRIP12), transcript variant X10, mRNA.

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CloneID	OCe43046
Clone ID Related Accession (Same CDS sequence)	XM_014107492.1 , XM_022409676.1
Accession Version	XM_014107492.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5997bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-09-16
Organism	Canis lupus familiaris(Dog)
Product	E3 ubiquitin-protein ligase TRIP12 isoform X9
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726103.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCCAACC GGCCTAATAA CAATCCAGGG GGGTCACTGC GACGTTCACA GAGGAACACT 
GCCGGGGCCC AACCACAAGA CGACTCGATA GGAGGAAGAA GCTGCAGTTC ATCATCTGCT 
GTCATAGTTC CACAACCAGA GGATCCAGAC AGAGCCAATA CTTCAGAAAG GCAAAAAACG 
GGGCAGGTGC CTAAGAAAGA CAATTCTCGA GGAGTGAAAC GCAGTGCTAG TCCAGACTAC 
AACAGGACCA ATTCTCCTAG CTCTGCCAAA AAACCAAAAG CACTTCAGCA TACTGAATCT 
CCCTCAGAAA CAAATAAGCC ACATAGTAAG TCGAAGAAGA GACATTTAGA CCAGGAGCAA 
CAACTGAAAT CTGCACAGTC GCCATCAACA AGTAAGGCTC ACACCAGAAA GAGTGGGGCC 
ACCGGCGGCT CACGGAGTCA GAAAAGAAAG AGGACAGAGA GTTCTTGTCT AAAGAGTGGT 
TCTGTGTCTG AGTCAACTGG TGCCGAAGAG AGATCTGCAA AACCCACCAA GCTGGCTTCA 
AAATCCGCCG CCTCGGCCAA AGCTGGGTGC AGCACCATCA CTGATTCTTC CTCCGCTGCC 
TCTACTTCCT CCTCATCTTC TGCTGTCGCC TCGGCCTCCT CCACTGTACC ACCAGGGGCC 
AGGGTGAAAC AAGGAAAAGA CCAGAGCAAG GCCAGGCGTT CCCGTTCAGC ATCCAGTCCC 
AGTCCCAGAA GAAGTAGCAG GGAAAAGGAA CAGAGTAAAA CTGGTGGCTC TTCAAAATTC 
GATTGGGCTG CTCGTTTCAG CCCTAAAGTT AGCCTTCCTA AAACCAAACT GTCTCTTCCA 
GGGTCTTCTA AGTCAGAGAC ATCAAAACCT GGACCTTCTG GATTACAGGC CAAATTAGCA 
AGTTTAAGAA AATCTACCAA GAAGCGCAGT GAATCCCCAC CTGCTGAGCT CCCCAGTTTG 
AGGCGGAGCA CACGCCAAAA GACCACGGGC TCCTGTGCTA GTGCCAGTCG GCGAGGCTCT 
GGCCTGGGCA AAAGAGGAGC AGCTGAAGCT CGTCGACAGG AGAAGATGGC GGACCCTGAA 
AGCAGCCAGG AGACCGTGAA TTCTTCAGCT GCACGGACAG ACGAAACTCC CCAAGGAGCT 
GCAGCCTCTA GTTCTGTTGC AGGGGCTGTG GGCATGACCA CCTCTGGGGA GAGTGAATCC 
GATGATTCTG AGATGGGACG ACTACAAGCT TTGTTAGAGG CCAGGGGTCT TCCTCCTCAT 
CTGTTTGGCC CTCTCGGTCC TCGGATGTCA CAACTTTTCC ATAGAACAAT TGGAAGCGGA 
GCTAGCTCTA AGGCCCAGCA GCTTCTGCAA GGACTACAAG CAAGTGATGA AAGCCAGCAA 
CTCCAGGCAG TTATTGAAAT GTGTCAGTTA TTGGTTATGG GAAATGAGGA GACGCTGGGA 
GGGTTTCCGG TCAAGAGTGT CGTTCCAGCT TTGATAACAT TACTGCAGAT GGAGCATAAT 
TTCGATATTA TGAACCATGC TTGTCGAGCC TTAACATATA TGATGGAAGC ACTTCCTCGG 
TCATCTGCTG TTGTAGTAGA TGCAATTCCT GTCTTTTTAG AAAAGCTGCA AGTTATTCAG 
TGTATTGATG TCGCAGAGCA GGCCCTGACT GCCTTGGAGA TGTTATCACG CAGACACAGT 
AAAGCCATTC TACAGGCGGG TGGTTTGGCA GACTGCTTGC TGTATCTAGA ATTCTTCAGC 
ATAAATGCCC AAAGAAATGC ACTAGCAATT GCAGCCAATT GCTGCCAGAG TATCACGCCA 
GATGAATTTC ATTTTGTGGC AGATTCCCTC CCGTTGCTTA CCCAAAGGCT AACCCATCAG 
GACAAAAAGT CAGTTGAAAG CACTTGCCTT TGTTTTGCAC GTCTAGTGGA CAACTTCCAA 
CATGAGGAGA ATTTACTCCA GCAGGTTGCC TCCAAAGATC TGCTTACGAA TGTTCAACAA 
CTCTTGGTAG TGACTCCACC CATTTTAAGT TCTGGGATGT TTATAATGGT GGTTCGCATG 
TTTTCTCTGA TGTGTTCCAA CTGTCCAACT TTAGCTGTTC AACTTATGAA GCAAAACATT 
GCAGAAACAC TTCACTTTCT CCTGTGTGGT GCCTCTAATG GAAGTTGTCA GGAACAGATT 
GATCTTGTTC CACGAAGTCC ACAAGAACTG TATGAACTGA CATCTCTGAT TTGCGAACTT 
ATGCCATGTT TACCAAAAGA AGGCATTTTT GCAGTTGATA CCATGCTGAA GAAGGGAAAT 
GCGCAGAACA CAGACGGTGC GATTTGGCAG TGGCGTGATG ACCGTGGCCT TTGGCATCCC 
TATAACAGGA TTGACAGCCG GATCATTGAG CAAATCAATG AGGACACGGG AACAGCACGT 
GCCATTCAGA GAAAACCTAA CCCCTTAGCC AATACTAACA CTAGTGGATA TTCAGAGTTA 
AAGAAGGATG ATGCTCGAGC ACAGCTTATG AAAGAGGATC CGGAACTGGC TAAGTCTTTT 
ATTAAGACAT TATTTGGTGT TCTTTATGAA GTGTATAGTT CCTCAGCAGG ACCTGCGGTC 
AGACATAAGT GCCTTAGAGC AATTCTTAGG ATAATTTATT TTGCGGATGC TGAACTTCTG 
AAGGATGTCC TGAAAAATCA TGCTGTTTCA AGTCACATTG CTTCCATGCT GTCAAGCCAA 
GACCTGAAGA TAGTAGTTGG AGCACTTCAG ATGGCAGAAA TCTTAATGCA GAAGTTACCT 
GATATTTTTA GTGTTTACTT CAGAAGAGAA GGTGTAATGC ATCAAGTAAA ACACTTAGCA 
GAATCAGAGT CTTTGTTGAC AAGTCCACCA AAGGCATGTA CGAATGGGTC AGGATCCCTG 
GGATCCACGA CGTCAGTCAG CAGTGGAACA GCCACGGCCG CCACCAATGC TGCAGCTGAC 
CTGGGGTCCC CCAGCTTGCA GCACAGCAGA GATGACTCTT TAGATCTGAG CCCTCAAGGT 
CGATTAAGTG ATGTTCTAAA GAGAAAACGG CTGCCAAAAC GAGGGCCAAG AAGGCCAAAG 
TATTCACCTC CAAGAGATGA TGACAAAGTA GACAATCAAG CTAAAAGCCC TACCACTACT 
CAGTCACCTA AATCTTCTTT CCTGGCAAGC TTGAATCCAA AAACGTGGGG AAGGTTAAGC 
GCACAGTCCA ACAGCAACAA CATCGAACCA GCACGAACTG CGGGAGTCAG TGGTCTTGCC 
AGGGCCGCCT CCAAGGACAC CATATCCAAT AACAGAGAAA AAATTAAAGG TTGGATTAAG 
GAACAGGCGC ATAAATTTGT AGAGCGTTAT TTCAGTTCTG AGAACATGGA TGGAAGCAAC 
CCTGCACTGA ATGTCCTTCA GAGACTTTGT GCTGCAACTG AACAACTCAA CCTCCAGGTG 
GATGGTGGAG CTGAGTGCCT TGTAGAAATC CGTAGCATAG TCTCAGAGTC TGATGTTTCA 
TCATTTGAAA TCCAACATAG TGGATTTGTG AAGCAGCTGT TACTTTATTT GACATCTAAA 
AGTGAAAAGG ATGCTGTGAG CCGAGAGATC AGATTAAAGA GATTCCTTCA CGTATTTTTT 
TCTTCACCAC TTCCTGGAGA AGAGCCCATT GGAAGAGTAG AACCAGTGGG TAATGCGCCT 
TTGTTGGCAT TAGTTCACAA GATGAACAAC TGCCTCAGCC AGATGGAACA GTTTCCAGTC 
AAAGTACATG ACTTCCCCAG TGGGAACGGG ACAGGAGGCA GCTTTTCTCT CAACAGAGGA 
TCACAGGCTT TAAAATTTTT CAACACACAC CAGTTAAAAT GCCAGTTACA AAGACATCCA 
GACTGTGCAA ATGTGAAGCA GTGGAAGGGC GGGCCGGTCA AGATTGATCC CCTGGCTTTG 
GTACAAGCTA TTGAGAGATA CCTTGTAGTT AGAGGGTATG GAAGAGTAAG AGAAGATGAT 
GAAGACAGTG ATGATGATGG CTCTGATGAG GAAATAGACG AGTCTCTGGC CGCTCAGTTC 
TTAAATTCCG GAAACGTAAG GCACAGGCTA CAGTTTTATA TTGGAGAACA TTTGCTACCA 
TATAACATGA CTGTATATCA GGCAGTCCGG CAGTTCAGTA TACAAGCTGA GGATGAGCGG 
GAGTCCACAG ATGATGAGAG CAACCCTCTG GGAAGAGCTG GTATTTGGAC AAAGACTCAT 
ACAATATGGT ACAAACCTGT GAGAGAGGAT GAAGAAAGTA ATAAAGATTG TGTTGGTGGT 
AAAAGAGGAA GAGCCCAAAC GGCTCCAACA AAAACATCCC CTAGAAATGC AAAAAAGCAT 
GATGAGTTAT GGCATGATGG AGTATGCCCA TCAGTATCCA ATCCTTTAGA AGTTTACCTC 
ATCTCCTCGC CACCTGAGAA TATAACTTTT GAAGACCCGT CCTTAGATGT GATTCTTCTT 
CTAAGAGTTT TACATGCCAT CAGCCGATAC TGGTATTACT TGTATGATAA TGCAATGTGC 
AAGGAGATTA TTCCAACTAG TGAATTTATT AACAGTAAGT TGACAGCAAA AGCAAATAGG 
CAGCTTCAAG ATCCTTTAGT AATCATGACA GGAAATATCC CAACGTGGCT TACTGAACTA 
GGAAAAACCT GCCCATTTTT CTTCCCTTTT GATACCCGGC AAATGCTTTT TTACGTAACT 
GCATTTGATC GGGACCGAGC AATGCAAAGA TTACTTGATA CCAACCCAGA AATCAACCAG 
TCGGATTCTC AAGATAGCAG AGTTGCACCT AGATTGGATA GAAAAAAACG TACTGTGAAC 
AGAGAGGAGC TGCTGAAACA AGCGGAGTCT GTAATGCAGG ATCTCGGTAG CTCACGGGCC 
ATGTTAGAAA TCCAGTATGA AAATGAGGTT GGTACAGGTC TTGGGCCTAC ACTGGAGTTT 
TATGCACTTG TATCTCAGGA ACTTCAGAGA GCTGACTTGG GTCTCTGGAG AGGTGAAGAA 
GTGACTCTCA GCAATCCAAA AGGAAGCCAA GAAGGGACCA AGTATATTCA AAACCTCCAG 
GGCCTGTTTG CACTTCCCTT TGGTAGGACA GCTAAGCCAG CCCATATCGC AAAGGTTAAA 
ATGAAGTTTC GCTTCTTAGG AAAATTAATG GCCAAGGCTA TCATGGATTT CAGATTGGTG 
GACCTTCCCC TTGGTTTACC CTTTTATAAG TGGATGCTAA GGCAAGAAAC TTCACTGACG 
TCGCATGATT TGTTTGACAT TGACCCAGTT GTAGCCAGAT CAGTGTATCA CCTAGAAGAC 
ATTGTCAGAC AGAAGAAAAG ACTTGAGCAA GATAAATCCC AGACCAAAGA GAGTCTACAA 
TATGCATTAG AAACCCTGAC TATGAATGGC TGCTCAGTTG AAGATCTAGG ATTGGATTTC 
ACGCTGCCAG GGTTTCCCAA CATTGAACTG AAGAAAGGAG GAAAGGATAT ACCAGTTACC 
ATCCACAATT TAGAGGAGTA TCTAAGACTG GTTATATTCT GGGCACTAAA TGAAGGCGTT 
TCCAGGCAAT TTGATTCCTT CAGAGATGGA TTTGAATCTG TCTTCCCACT TAGTCATCTT 
CAGTACTTCT ACCCGGAAGA ACTGGATCAG CTCTTGTGCG GCAGTAAAGC AGACACGTGG 
GATGCGAAGA CACTGATGGA GTGCTGCAGG CCGGACCACG GTTATACTCA TGACAGTCGG 
GCTGTGAAGT TCTTGTTTGA GATTCTCAGT AGTTTTGATA ATGAGCAGCA GAGGTTGTTT 
CTCCAGTTTG TGACAGGTAG CCCAAGATTG CCTGTTGGAG GCTTCCGGAG TCTGAATCCA 
CCTCTGACAA TTGTCCGGAA GACCTTCGAG TCCACGGAGA ACCCCGACGA CTTCTTGCCC 
TCTGTAATGA CTTGTGTGAA CTACCTTAAG TTGCCGGACT ACTCGAGCAT TGACATCATG 
CGTGAGAAAC TGTTGATTGC AGCGAGAGAA GGGCAGCAGT CATTCCATCT TTCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_013962967.1
CDS	50..6046
Translation	MSNRPNNNPGGSLRRSQRNTAGAQPQDDSIGGRSCSSSSAVIVPQPEDPDRANTSERQKTGQVPKKDNSRGVKRSASPDYNRTNSPSSAKKPKALQHTESPSETNKPHSKSKKRHLDQEQQLKSAQSPSTSKAHTRKSGATGGSRSQKRKRTESSCLKSGSVSESTGAEERSAKPTKLASKSAASAKAGCSTITDSSSAASTSSSSSAVASASSTVPPGARVKQGKDQSKARRSRSASSPSPRRSSREKEQSKTGGSSKFDWAARFSPKVSLPKTKLSLPGSSKSETSKPGPSGLQAKLASLRKSTKKRSESPPAELPSLRRSTRQKTTGSCASASRRGSGLGKRGAAEARRQEKMADPESSQETVNSSAARTDETPQGAAASSSVAGAVGMTTSGESESDDSEMGRLQALLEARGLPPHLFGPLGPRMSQLFHRTIGSGASSKAQQLLQGLQASDESQQLQAVIEMCQLLVMGNEETLGGFPVKSVVPALITLLQMEHNFDIMNHACRALTYMMEALPRSSAVVVDAIPVFLEKLQVIQCIDVAEQALTALEMLSRRHSKAILQAGGLADCLLYLEFFSINAQRNALAIAANCCQSITPDEFHFVADSLPLLTQRLTHQDKKSVESTCLCFARLVDNFQHEENLLQQVASKDLLTNVQQLLVVTPPILSSGMFIMVVRMFSLMCSNCPTLAVQLMKQNIAETLHFLLCGASNGSCQEQIDLVPRSPQELYELTSLICELMPCLPKEGIFAVDTMLKKGNAQNTDGAIWQWRDDRGLWHPYNRIDSRIIEQINEDTGTARAIQRKPNPLANTNTSGYSELKKDDARAQLMKEDPELAKSFIKTLFGVLYEVYSSSAGPAVRHKCLRAILRIIYFADAELLKDVLKNHAVSSHIASMLSSQDLKIVVGALQMAEILMQKLPDIFSVYFRREGVMHQVKHLAESESLLTSPPKACTNGSGSLGSTTSVSSGTATAATNAAADLGSPSLQHSRDDSLDLSPQGRLSDVLKRKRLPKRGPRRPKYSPPRDDDKVDNQAKSPTTTQSPKSSFLASLNPKTWGRLSAQSNSNNIEPARTAGVSGLARAASKDTISNNREKIKGWIKEQAHKFVERYFSSENMDGSNPALNVLQRLCAATEQLNLQVDGGAECLVEIRSIVSESDVSSFEIQHSGFVKQLLLYLTSKSEKDAVSREIRLKRFLHVFFSSPLPGEEPIGRVEPVGNAPLLALVHKMNNCLSQMEQFPVKVHDFPSGNGTGGSFSLNRGSQALKFFNTHQLKCQLQRHPDCANVKQWKGGPVKIDPLALVQAIERYLVVRGYGRVREDDEDSDDDGSDEEIDESLAAQFLNSGNVRHRLQFYIGEHLLPYNMTVYQAVRQFSIQAEDERESTDDESNPLGRAGIWTKTHTIWYKPVREDEESNKDCVGGKRGRAQTAPTKTSPRNAKKHDELWHDGVCPSVSNPLEVYLISSPPENITFEDPSLDVILLLRVLHAISRYWYYLYDNAMCKEIIPTSEFINSKLTAKANRQLQDPLVIMTGNIPTWLTELGKTCPFFFPFDTRQMLFYVTAFDRDRAMQRLLDTNPEINQSDSQDSRVAPRLDRKKRTVNREELLKQAESVMQDLGSSRAMLEIQYENEVGTGLGPTLEFYALVSQELQRADLGLWRGEEVTLSNPKGSQEGTKYIQNLQGLFALPFGRTAKPAHIAKVKMKFRFLGKLMAKAIMDFRLVDLPLGLPFYKWMLRQETSLTSHDLFDIDPVVARSVYHLEDIVRQKKRLEQDKSQTKESLQYALETLTMNGCSVEDLGLDFTLPGFPNIELKKGGKDIPVTIHNLEEYLRLVIFWALNEGVSRQFDSFRDGFESVFPLSHLQYFYPEELDQLLCGSKADTWDAKTLMECCRPDHGYTHDSRAVKFLFEILSSFDNEQQRLFLQFVTGSPRLPVGGFRSLNPPLTIVRKTFESTENPDDFLPSVMTCVNYLKLPDYSSIDIMREKLLIAAREGQQSFHLS

Target ORF information:

RefSeq Version	XM_014107492.1
Organism	Canis lupus familiaris(Dog)
Definition	Canis lupus familiaris thyroid hormone receptor interactor 12 (TRIP12), transcript variant X10, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_014107492.1

ORF Insert Sequence:

ATGTCCAACC GGCCTAATAA CAATCCAGGG GGGTCACTGC GACGTTCACA GAGGAACACT 
GCCGGGGCCC AACCACAAGA CGACTCGATA GGAGGAAGAA GCTGCAGTTC ATCATCTGCT 
GTCATAGTTC CACAACCAGA GGATCCAGAC AGAGCCAATA CTTCAGAAAG GCAAAAAACG 
GGGCAGGTGC CTAAGAAAGA CAATTCTCGA GGAGTGAAAC GCAGTGCTAG TCCAGACTAC 
AACAGGACCA ATTCTCCTAG CTCTGCCAAA AAACCAAAAG CACTTCAGCA TACTGAATCT 
CCCTCAGAAA CAAATAAGCC ACATAGTAAG TCGAAGAAGA GACATTTAGA CCAGGAGCAA 
CAACTGAAAT CTGCACAGTC GCCATCAACA AGTAAGGCTC ACACCAGAAA GAGTGGGGCC 
ACCGGCGGCT CACGGAGTCA GAAAAGAAAG AGGACAGAGA GTTCTTGTCT AAAGAGTGGT 
TCTGTGTCTG AGTCAACTGG TGCCGAAGAG AGATCTGCAA AACCCACCAA GCTGGCTTCA 
AAATCCGCCG CCTCGGCCAA AGCTGGGTGC AGCACCATCA CTGATTCTTC CTCCGCTGCC 
TCTACTTCCT CCTCATCTTC TGCTGTCGCC TCGGCCTCCT CCACTGTACC ACCAGGGGCC 
AGGGTGAAAC AAGGAAAAGA CCAGAGCAAG GCCAGGCGTT CCCGTTCAGC ATCCAGTCCC 
AGTCCCAGAA GAAGTAGCAG GGAAAAGGAA CAGAGTAAAA CTGGTGGCTC TTCAAAATTC 
GATTGGGCTG CTCGTTTCAG CCCTAAAGTT AGCCTTCCTA AAACCAAACT GTCTCTTCCA 
GGGTCTTCTA AGTCAGAGAC ATCAAAACCT GGACCTTCTG GATTACAGGC CAAATTAGCA 
AGTTTAAGAA AATCTACCAA GAAGCGCAGT GAATCCCCAC CTGCTGAGCT CCCCAGTTTG 
AGGCGGAGCA CACGCCAAAA GACCACGGGC TCCTGTGCTA GTGCCAGTCG GCGAGGCTCT 
GGCCTGGGCA AAAGAGGAGC AGCTGAAGCT CGTCGACAGG AGAAGATGGC GGACCCTGAA 
AGCAGCCAGG AGACCGTGAA TTCTTCAGCT GCACGGACAG ACGAAACTCC CCAAGGAGCT 
GCAGCCTCTA GTTCTGTTGC AGGGGCTGTG GGCATGACCA CCTCTGGGGA GAGTGAATCC 
GATGATTCTG AGATGGGACG ACTACAAGCT TTGTTAGAGG CCAGGGGTCT TCCTCCTCAT 
CTGTTTGGCC CTCTCGGTCC TCGGATGTCA CAACTTTTCC ATAGAACAAT TGGAAGCGGA 
GCTAGCTCTA AGGCCCAGCA GCTTCTGCAA GGACTACAAG CAAGTGATGA AAGCCAGCAA 
CTCCAGGCAG TTATTGAAAT GTGTCAGTTA TTGGTTATGG GAAATGAGGA GACGCTGGGA 
GGGTTTCCGG TCAAGAGTGT CGTTCCAGCT TTGATAACAT TACTGCAGAT GGAGCATAAT 
TTCGATATTA TGAACCATGC TTGTCGAGCC TTAACATATA TGATGGAAGC ACTTCCTCGG 
TCATCTGCTG TTGTAGTAGA TGCAATTCCT GTCTTTTTAG AAAAGCTGCA AGTTATTCAG 
TGTATTGATG TCGCAGAGCA GGCCCTGACT GCCTTGGAGA TGTTATCACG CAGACACAGT 
AAAGCCATTC TACAGGCGGG TGGTTTGGCA GACTGCTTGC TGTATCTAGA ATTCTTCAGC 
ATAAATGCCC AAAGAAATGC ACTAGCAATT GCAGCCAATT GCTGCCAGAG TATCACGCCA 
GATGAATTTC ATTTTGTGGC AGATTCCCTC CCGTTGCTTA CCCAAAGGCT AACCCATCAG 
GACAAAAAGT CAGTTGAAAG CACTTGCCTT TGTTTTGCAC GTCTAGTGGA CAACTTCCAA 
CATGAGGAGA ATTTACTCCA GCAGGTTGCC TCCAAAGATC TGCTTACGAA TGTTCAACAA 
CTCTTGGTAG TGACTCCACC CATTTTAAGT TCTGGGATGT TTATAATGGT GGTTCGCATG 
TTTTCTCTGA TGTGTTCCAA CTGTCCAACT TTAGCTGTTC AACTTATGAA GCAAAACATT 
GCAGAAACAC TTCACTTTCT CCTGTGTGGT GCCTCTAATG GAAGTTGTCA GGAACAGATT 
GATCTTGTTC CACGAAGTCC ACAAGAACTG TATGAACTGA CATCTCTGAT TTGCGAACTT 
ATGCCATGTT TACCAAAAGA AGGCATTTTT GCAGTTGATA CCATGCTGAA GAAGGGAAAT 
GCGCAGAACA CAGACGGTGC GATTTGGCAG TGGCGTGATG ACCGTGGCCT TTGGCATCCC 
TATAACAGGA TTGACAGCCG GATCATTGAG CAAATCAATG AGGACACGGG AACAGCACGT 
GCCATTCAGA GAAAACCTAA CCCCTTAGCC AATACTAACA CTAGTGGATA TTCAGAGTTA 
AAGAAGGATG ATGCTCGAGC ACAGCTTATG AAAGAGGATC CGGAACTGGC TAAGTCTTTT 
ATTAAGACAT TATTTGGTGT TCTTTATGAA GTGTATAGTT CCTCAGCAGG ACCTGCGGTC 
AGACATAAGT GCCTTAGAGC AATTCTTAGG ATAATTTATT TTGCGGATGC TGAACTTCTG 
AAGGATGTCC TGAAAAATCA TGCTGTTTCA AGTCACATTG CTTCCATGCT GTCAAGCCAA 
GACCTGAAGA TAGTAGTTGG AGCACTTCAG ATGGCAGAAA TCTTAATGCA GAAGTTACCT 
GATATTTTTA GTGTTTACTT CAGAAGAGAA GGTGTAATGC ATCAAGTAAA ACACTTAGCA 
GAATCAGAGT CTTTGTTGAC AAGTCCACCA AAGGCATGTA CGAATGGGTC AGGATCCCTG 
GGATCCACGA CGTCAGTCAG CAGTGGAACA GCCACGGCCG CCACCAATGC TGCAGCTGAC 
CTGGGGTCCC CCAGCTTGCA GCACAGCAGA GATGACTCTT TAGATCTGAG CCCTCAAGGT 
CGATTAAGTG ATGTTCTAAA GAGAAAACGG CTGCCAAAAC GAGGGCCAAG AAGGCCAAAG 
TATTCACCTC CAAGAGATGA TGACAAAGTA GACAATCAAG CTAAAAGCCC TACCACTACT 
CAGTCACCTA AATCTTCTTT CCTGGCAAGC TTGAATCCAA AAACGTGGGG AAGGTTAAGC 
GCACAGTCCA ACAGCAACAA CATCGAACCA GCACGAACTG CGGGAGTCAG TGGTCTTGCC 
AGGGCCGCCT CCAAGGACAC CATATCCAAT AACAGAGAAA AAATTAAAGG TTGGATTAAG 
GAACAGGCGC ATAAATTTGT AGAGCGTTAT TTCAGTTCTG AGAACATGGA TGGAAGCAAC 
CCTGCACTGA ATGTCCTTCA GAGACTTTGT GCTGCAACTG AACAACTCAA CCTCCAGGTG 
GATGGTGGAG CTGAGTGCCT TGTAGAAATC CGTAGCATAG TCTCAGAGTC TGATGTTTCA 
TCATTTGAAA TCCAACATAG TGGATTTGTG AAGCAGCTGT TACTTTATTT GACATCTAAA 
AGTGAAAAGG ATGCTGTGAG CCGAGAGATC AGATTAAAGA GATTCCTTCA CGTATTTTTT 
TCTTCACCAC TTCCTGGAGA AGAGCCCATT GGAAGAGTAG AACCAGTGGG TAATGCGCCT 
TTGTTGGCAT TAGTTCACAA GATGAACAAC TGCCTCAGCC AGATGGAACA GTTTCCAGTC 
AAAGTACATG ACTTCCCCAG TGGGAACGGG ACAGGAGGCA GCTTTTCTCT CAACAGAGGA 
TCACAGGCTT TAAAATTTTT CAACACACAC CAGTTAAAAT GCCAGTTACA AAGACATCCA 
GACTGTGCAA ATGTGAAGCA GTGGAAGGGC GGGCCGGTCA AGATTGATCC CCTGGCTTTG 
GTACAAGCTA TTGAGAGATA CCTTGTAGTT AGAGGGTATG GAAGAGTAAG AGAAGATGAT 
GAAGACAGTG ATGATGATGG CTCTGATGAG GAAATAGACG AGTCTCTGGC CGCTCAGTTC 
TTAAATTCCG GAAACGTAAG GCACAGGCTA CAGTTTTATA TTGGAGAACA TTTGCTACCA 
TATAACATGA CTGTATATCA GGCAGTCCGG CAGTTCAGTA TACAAGCTGA GGATGAGCGG 
GAGTCCACAG ATGATGAGAG CAACCCTCTG GGAAGAGCTG GTATTTGGAC AAAGACTCAT 
ACAATATGGT ACAAACCTGT GAGAGAGGAT GAAGAAAGTA ATAAAGATTG TGTTGGTGGT 
AAAAGAGGAA GAGCCCAAAC GGCTCCAACA AAAACATCCC CTAGAAATGC AAAAAAGCAT 
GATGAGTTAT GGCATGATGG AGTATGCCCA TCAGTATCCA ATCCTTTAGA AGTTTACCTC 
ATCTCCTCGC CACCTGAGAA TATAACTTTT GAAGACCCGT CCTTAGATGT GATTCTTCTT 
CTAAGAGTTT TACATGCCAT CAGCCGATAC TGGTATTACT TGTATGATAA TGCAATGTGC 
AAGGAGATTA TTCCAACTAG TGAATTTATT AACAGTAAGT TGACAGCAAA AGCAAATAGG 
CAGCTTCAAG ATCCTTTAGT AATCATGACA GGAAATATCC CAACGTGGCT TACTGAACTA 
GGAAAAACCT GCCCATTTTT CTTCCCTTTT GATACCCGGC AAATGCTTTT TTACGTAACT 
GCATTTGATC GGGACCGAGC AATGCAAAGA TTACTTGATA CCAACCCAGA AATCAACCAG 
TCGGATTCTC AAGATAGCAG AGTTGCACCT AGATTGGATA GAAAAAAACG TACTGTGAAC 
AGAGAGGAGC TGCTGAAACA AGCGGAGTCT GTAATGCAGG ATCTCGGTAG CTCACGGGCC 
ATGTTAGAAA TCCAGTATGA AAATGAGGTT GGTACAGGTC TTGGGCCTAC ACTGGAGTTT 
TATGCACTTG TATCTCAGGA ACTTCAGAGA GCTGACTTGG GTCTCTGGAG AGGTGAAGAA 
GTGACTCTCA GCAATCCAAA AGGAAGCCAA GAAGGGACCA AGTATATTCA AAACCTCCAG 
GGCCTGTTTG CACTTCCCTT TGGTAGGACA GCTAAGCCAG CCCATATCGC AAAGGTTAAA 
ATGAAGTTTC GCTTCTTAGG AAAATTAATG GCCAAGGCTA TCATGGATTT CAGATTGGTG 
GACCTTCCCC TTGGTTTACC CTTTTATAAG TGGATGCTAA GGCAAGAAAC TTCACTGACG 
TCGCATGATT TGTTTGACAT TGACCCAGTT GTAGCCAGAT CAGTGTATCA CCTAGAAGAC 
ATTGTCAGAC AGAAGAAAAG ACTTGAGCAA GATAAATCCC AGACCAAAGA GAGTCTACAA 
TATGCATTAG AAACCCTGAC TATGAATGGC TGCTCAGTTG AAGATCTAGG ATTGGATTTC 
ACGCTGCCAG GGTTTCCCAA CATTGAACTG AAGAAAGGAG GAAAGGATAT ACCAGTTACC 
ATCCACAATT TAGAGGAGTA TCTAAGACTG GTTATATTCT GGGCACTAAA TGAAGGCGTT 
TCCAGGCAAT TTGATTCCTT CAGAGATGGA TTTGAATCTG TCTTCCCACT TAGTCATCTT 
CAGTACTTCT ACCCGGAAGA ACTGGATCAG CTCTTGTGCG GCAGTAAAGC AGACACGTGG 
GATGCGAAGA CACTGATGGA GTGCTGCAGG CCGGACCACG GTTATACTCA TGACAGTCGG 
GCTGTGAAGT TCTTGTTTGA GATTCTCAGT AGTTTTGATA ATGAGCAGCA GAGGTTGTTT 
CTCCAGTTTG TGACAGGTAG CCCAAGATTG CCTGTTGGAG GCTTCCGGAG TCTGAATCCA 
CCTCTGACAA TTGTCCGGAA GACCTTCGAG TCCACGGAGA ACCCCGACGA CTTCTTGCCC 
TCTGTAATGA CTTGTGTGAA CTACCTTAAG TTGCCGGACT ACTCGAGCAT TGACATCATG 
CGTGAGAAAC TGTTGATTGC AGCGAGAGAA GGGCAGCAGT CATTCCATCT TTCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TRIP12 ( XM_022409676.1 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_022265384.1 Canis lupus familiaris thyroid hormone receptor interactor 12 (TRIP12), transcript variant X16, mRNA.

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CloneID	OCe43046
Clone ID Related Accession (Same CDS sequence)	XM_014107492.1 , XM_022409676.1
Accession Version	XM_022409676.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5997bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-09-04
Organism	Canis lupus familiaris(dog)
Product	E3 ubiquitin-protein ligase TRIP12 isoform X14
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006607.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCCAACC GGCCTAATAA CAATCCAGGG GGGTCACTGC GACGTTCACA GAGGAACACT 
GCCGGGGCCC AACCACAAGA CGACTCGATA GGAGGAAGAA GCTGCAGTTC ATCATCTGCT 
GTCATAGTTC CACAACCAGA GGATCCAGAC AGAGCCAATA CTTCAGAAAG GCAAAAAACG 
GGGCAGGTGC CTAAGAAAGA CAATTCTCGA GGAGTGAAAC GCAGTGCTAG TCCAGACTAC 
AACAGGACCA ATTCTCCTAG CTCTGCCAAA AAACCAAAAG CACTTCAGCA TACTGAATCT 
CCCTCAGAAA CAAATAAGCC ACATAGTAAG TCGAAGAAGA GACATTTAGA CCAGGAGCAA 
CAACTGAAAT CTGCACAGTC GCCATCAACA AGTAAGGCTC ACACCAGAAA GAGTGGGGCC 
ACCGGCGGCT CACGGAGTCA GAAAAGAAAG AGGACAGAGA GTTCTTGTCT AAAGAGTGGT 
TCTGTGTCTG AGTCAACTGG TGCCGAAGAG AGATCTGCAA AACCCACCAA GCTGGCTTCA 
AAATCCGCCG CCTCGGCCAA AGCTGGGTGC AGCACCATCA CTGATTCTTC CTCCGCTGCC 
TCTACTTCCT CCTCATCTTC TGCTGTCGCC TCGGCCTCCT CCACTGTACC ACCAGGGGCC 
AGGGTGAAAC AAGGAAAAGA CCAGAGCAAG GCCAGGCGTT CCCGTTCAGC ATCCAGTCCC 
AGTCCCAGAA GAAGTAGCAG GGAAAAGGAA CAGAGTAAAA CTGGTGGCTC TTCAAAATTC 
GATTGGGCTG CTCGTTTCAG CCCTAAAGTT AGCCTTCCTA AAACCAAACT GTCTCTTCCA 
GGGTCTTCTA AGTCAGAGAC ATCAAAACCT GGACCTTCTG GATTACAGGC CAAATTAGCA 
AGTTTAAGAA AATCTACCAA GAAGCGCAGT GAATCCCCAC CTGCTGAGCT CCCCAGTTTG 
AGGCGGAGCA CACGCCAAAA GACCACGGGC TCCTGTGCTA GTGCCAGTCG GCGAGGCTCT 
GGCCTGGGCA AAAGAGGAGC AGCTGAAGCT CGTCGACAGG AGAAGATGGC GGACCCTGAA 
AGCAGCCAGG AGACCGTGAA TTCTTCAGCT GCACGGACAG ACGAAACTCC CCAAGGAGCT 
GCAGCCTCTA GTTCTGTTGC AGGGGCTGTG GGCATGACCA CCTCTGGGGA GAGTGAATCC 
GATGATTCTG AGATGGGACG ACTACAAGCT TTGTTAGAGG CCAGGGGTCT TCCTCCTCAT 
CTGTTTGGCC CTCTCGGTCC TCGGATGTCA CAACTTTTCC ATAGAACAAT TGGAAGCGGA 
GCTAGCTCTA AGGCCCAGCA GCTTCTGCAA GGACTACAAG CAAGTGATGA AAGCCAGCAA 
CTCCAGGCAG TTATTGAAAT GTGTCAGTTA TTGGTTATGG GAAATGAGGA GACGCTGGGA 
GGGTTTCCGG TCAAGAGTGT CGTTCCAGCT TTGATAACAT TACTGCAGAT GGAGCATAAT 
TTCGATATTA TGAACCATGC TTGTCGAGCC TTAACATATA TGATGGAAGC ACTTCCTCGG 
TCATCTGCTG TTGTAGTAGA TGCAATTCCT GTCTTTTTAG AAAAGCTGCA AGTTATTCAG 
TGTATTGATG TCGCAGAGCA GGCCCTGACT GCCTTGGAGA TGTTATCACG CAGACACAGT 
AAAGCCATTC TACAGGCGGG TGGTTTGGCA GACTGCTTGC TGTATCTAGA ATTCTTCAGC 
ATAAATGCCC AAAGAAATGC ACTAGCAATT GCAGCCAATT GCTGCCAGAG TATCACGCCA 
GATGAATTTC ATTTTGTGGC AGATTCCCTC CCGTTGCTTA CCCAAAGGCT AACCCATCAG 
GACAAAAAGT CAGTTGAAAG CACTTGCCTT TGTTTTGCAC GTCTAGTGGA CAACTTCCAA 
CATGAGGAGA ATTTACTCCA GCAGGTTGCC TCCAAAGATC TGCTTACGAA TGTTCAACAA 
CTCTTGGTAG TGACTCCACC CATTTTAAGT TCTGGGATGT TTATAATGGT GGTTCGCATG 
TTTTCTCTGA TGTGTTCCAA CTGTCCAACT TTAGCTGTTC AACTTATGAA GCAAAACATT 
GCAGAAACAC TTCACTTTCT CCTGTGTGGT GCCTCTAATG GAAGTTGTCA GGAACAGATT 
GATCTTGTTC CACGAAGTCC ACAAGAACTG TATGAACTGA CATCTCTGAT TTGCGAACTT 
ATGCCATGTT TACCAAAAGA AGGCATTTTT GCAGTTGATA CCATGCTGAA GAAGGGAAAT 
GCGCAGAACA CAGACGGTGC GATTTGGCAG TGGCGTGATG ACCGTGGCCT TTGGCATCCC 
TATAACAGGA TTGACAGCCG GATCATTGAG CAAATCAATG AGGACACGGG AACAGCACGT 
GCCATTCAGA GAAAACCTAA CCCCTTAGCC AATACTAACA CTAGTGGATA TTCAGAGTTA 
AAGAAGGATG ATGCTCGAGC ACAGCTTATG AAAGAGGATC CGGAACTGGC TAAGTCTTTT 
ATTAAGACAT TATTTGGTGT TCTTTATGAA GTGTATAGTT CCTCAGCAGG ACCTGCGGTC 
AGACATAAGT GCCTTAGAGC AATTCTTAGG ATAATTTATT TTGCGGATGC TGAACTTCTG 
AAGGATGTCC TGAAAAATCA TGCTGTTTCA AGTCACATTG CTTCCATGCT GTCAAGCCAA 
GACCTGAAGA TAGTAGTTGG AGCACTTCAG ATGGCAGAAA TCTTAATGCA GAAGTTACCT 
GATATTTTTA GTGTTTACTT CAGAAGAGAA GGTGTAATGC ATCAAGTAAA ACACTTAGCA 
GAATCAGAGT CTTTGTTGAC AAGTCCACCA AAGGCATGTA CGAATGGGTC AGGATCCCTG 
GGATCCACGA CGTCAGTCAG CAGTGGAACA GCCACGGCCG CCACCAATGC TGCAGCTGAC 
CTGGGGTCCC CCAGCTTGCA GCACAGCAGA GATGACTCTT TAGATCTGAG CCCTCAAGGT 
CGATTAAGTG ATGTTCTAAA GAGAAAACGG CTGCCAAAAC GAGGGCCAAG AAGGCCAAAG 
TATTCACCTC CAAGAGATGA TGACAAAGTA GACAATCAAG CTAAAAGCCC TACCACTACT 
CAGTCACCTA AATCTTCTTT CCTGGCAAGC TTGAATCCAA AAACGTGGGG AAGGTTAAGC 
GCACAGTCCA ACAGCAACAA CATCGAACCA GCACGAACTG CGGGAGTCAG TGGTCTTGCC 
AGGGCCGCCT CCAAGGACAC CATATCCAAT AACAGAGAAA AAATTAAAGG TTGGATTAAG 
GAACAGGCGC ATAAATTTGT AGAGCGTTAT TTCAGTTCTG AGAACATGGA TGGAAGCAAC 
CCTGCACTGA ATGTCCTTCA GAGACTTTGT GCTGCAACTG AACAACTCAA CCTCCAGGTG 
GATGGTGGAG CTGAGTGCCT TGTAGAAATC CGTAGCATAG TCTCAGAGTC TGATGTTTCA 
TCATTTGAAA TCCAACATAG TGGATTTGTG AAGCAGCTGT TACTTTATTT GACATCTAAA 
AGTGAAAAGG ATGCTGTGAG CCGAGAGATC AGATTAAAGA GATTCCTTCA CGTATTTTTT 
TCTTCACCAC TTCCTGGAGA AGAGCCCATT GGAAGAGTAG AACCAGTGGG TAATGCGCCT 
TTGTTGGCAT TAGTTCACAA GATGAACAAC TGCCTCAGCC AGATGGAACA GTTTCCAGTC 
AAAGTACATG ACTTCCCCAG TGGGAACGGG ACAGGAGGCA GCTTTTCTCT CAACAGAGGA 
TCACAGGCTT TAAAATTTTT CAACACACAC CAGTTAAAAT GCCAGTTACA AAGACATCCA 
GACTGTGCAA ATGTGAAGCA GTGGAAGGGC GGGCCGGTCA AGATTGATCC CCTGGCTTTG 
GTACAAGCTA TTGAGAGATA CCTTGTAGTT AGAGGGTATG GAAGAGTAAG AGAAGATGAT 
GAAGACAGTG ATGATGATGG CTCTGATGAG GAAATAGACG AGTCTCTGGC CGCTCAGTTC 
TTAAATTCCG GAAACGTAAG GCACAGGCTA CAGTTTTATA TTGGAGAACA TTTGCTACCA 
TATAACATGA CTGTATATCA GGCAGTCCGG CAGTTCAGTA TACAAGCTGA GGATGAGCGG 
GAGTCCACAG ATGATGAGAG CAACCCTCTG GGAAGAGCTG GTATTTGGAC AAAGACTCAT 
ACAATATGGT ACAAACCTGT GAGAGAGGAT GAAGAAAGTA ATAAAGATTG TGTTGGTGGT 
AAAAGAGGAA GAGCCCAAAC GGCTCCAACA AAAACATCCC CTAGAAATGC AAAAAAGCAT 
GATGAGTTAT GGCATGATGG AGTATGCCCA TCAGTATCCA ATCCTTTAGA AGTTTACCTC 
ATCTCCTCGC CACCTGAGAA TATAACTTTT GAAGACCCGT CCTTAGATGT GATTCTTCTT 
CTAAGAGTTT TACATGCCAT CAGCCGATAC TGGTATTACT TGTATGATAA TGCAATGTGC 
AAGGAGATTA TTCCAACTAG TGAATTTATT AACAGTAAGT TGACAGCAAA AGCAAATAGG 
CAGCTTCAAG ATCCTTTAGT AATCATGACA GGAAATATCC CAACGTGGCT TACTGAACTA 
GGAAAAACCT GCCCATTTTT CTTCCCTTTT GATACCCGGC AAATGCTTTT TTACGTAACT 
GCATTTGATC GGGACCGAGC AATGCAAAGA TTACTTGATA CCAACCCAGA AATCAACCAG 
TCGGATTCTC AAGATAGCAG AGTTGCACCT AGATTGGATA GAAAAAAACG TACTGTGAAC 
AGAGAGGAGC TGCTGAAACA AGCGGAGTCT GTAATGCAGG ATCTCGGTAG CTCACGGGCC 
ATGTTAGAAA TCCAGTATGA AAATGAGGTT GGTACAGGTC TTGGGCCTAC ACTGGAGTTT 
TATGCACTTG TATCTCAGGA ACTTCAGAGA GCTGACTTGG GTCTCTGGAG AGGTGAAGAA 
GTGACTCTCA GCAATCCAAA AGGAAGCCAA GAAGGGACCA AGTATATTCA AAACCTCCAG 
GGCCTGTTTG CACTTCCCTT TGGTAGGACA GCTAAGCCAG CCCATATCGC AAAGGTTAAA 
ATGAAGTTTC GCTTCTTAGG AAAATTAATG GCCAAGGCTA TCATGGATTT CAGATTGGTG 
GACCTTCCCC TTGGTTTACC CTTTTATAAG TGGATGCTAA GGCAAGAAAC TTCACTGACG 
TCGCATGATT TGTTTGACAT TGACCCAGTT GTAGCCAGAT CAGTGTATCA CCTAGAAGAC 
ATTGTCAGAC AGAAGAAAAG ACTTGAGCAA GATAAATCCC AGACCAAAGA GAGTCTACAA 
TATGCATTAG AAACCCTGAC TATGAATGGC TGCTCAGTTG AAGATCTAGG ATTGGATTTC 
ACGCTGCCAG GGTTTCCCAA CATTGAACTG AAGAAAGGAG GAAAGGATAT ACCAGTTACC 
ATCCACAATT TAGAGGAGTA TCTAAGACTG GTTATATTCT GGGCACTAAA TGAAGGCGTT 
TCCAGGCAAT TTGATTCCTT CAGAGATGGA TTTGAATCTG TCTTCCCACT TAGTCATCTT 
CAGTACTTCT ACCCGGAAGA ACTGGATCAG CTCTTGTGCG GCAGTAAAGC AGACACGTGG 
GATGCGAAGA CACTGATGGA GTGCTGCAGG CCGGACCACG GTTATACTCA TGACAGTCGG 
GCTGTGAAGT TCTTGTTTGA GATTCTCAGT AGTTTTGATA ATGAGCAGCA GAGGTTGTTT 
CTCCAGTTTG TGACAGGTAG CCCAAGATTG CCTGTTGGAG GCTTCCGGAG TCTGAATCCA 
CCTCTGACAA TTGTCCGGAA GACCTTCGAG TCCACGGAGA ACCCCGACGA CTTCTTGCCC 
TCTGTAATGA CTTGTGTGAA CTACCTTAAG TTGCCGGACT ACTCGAGCAT TGACATCATG 
CGTGAGAAAC TGTTGATTGC AGCGAGAGAA GGGCAGCAGT CATTCCATCT TTCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_022265384.1
CDS	160..6156
Translation	MSNRPNNNPGGSLRRSQRNTAGAQPQDDSIGGRSCSSSSAVIVPQPEDPDRANTSERQKTGQVPKKDNSRGVKRSASPDYNRTNSPSSAKKPKALQHTESPSETNKPHSKSKKRHLDQEQQLKSAQSPSTSKAHTRKSGATGGSRSQKRKRTESSCLKSGSVSESTGAEERSAKPTKLASKSAASAKAGCSTITDSSSAASTSSSSSAVASASSTVPPGARVKQGKDQSKARRSRSASSPSPRRSSREKEQSKTGGSSKFDWAARFSPKVSLPKTKLSLPGSSKSETSKPGPSGLQAKLASLRKSTKKRSESPPAELPSLRRSTRQKTTGSCASASRRGSGLGKRGAAEARRQEKMADPESSQETVNSSAARTDETPQGAAASSSVAGAVGMTTSGESESDDSEMGRLQALLEARGLPPHLFGPLGPRMSQLFHRTIGSGASSKAQQLLQGLQASDESQQLQAVIEMCQLLVMGNEETLGGFPVKSVVPALITLLQMEHNFDIMNHACRALTYMMEALPRSSAVVVDAIPVFLEKLQVIQCIDVAEQALTALEMLSRRHSKAILQAGGLADCLLYLEFFSINAQRNALAIAANCCQSITPDEFHFVADSLPLLTQRLTHQDKKSVESTCLCFARLVDNFQHEENLLQQVASKDLLTNVQQLLVVTPPILSSGMFIMVVRMFSLMCSNCPTLAVQLMKQNIAETLHFLLCGASNGSCQEQIDLVPRSPQELYELTSLICELMPCLPKEGIFAVDTMLKKGNAQNTDGAIWQWRDDRGLWHPYNRIDSRIIEQINEDTGTARAIQRKPNPLANTNTSGYSELKKDDARAQLMKEDPELAKSFIKTLFGVLYEVYSSSAGPAVRHKCLRAILRIIYFADAELLKDVLKNHAVSSHIASMLSSQDLKIVVGALQMAEILMQKLPDIFSVYFRREGVMHQVKHLAESESLLTSPPKACTNGSGSLGSTTSVSSGTATAATNAAADLGSPSLQHSRDDSLDLSPQGRLSDVLKRKRLPKRGPRRPKYSPPRDDDKVDNQAKSPTTTQSPKSSFLASLNPKTWGRLSAQSNSNNIEPARTAGVSGLARAASKDTISNNREKIKGWIKEQAHKFVERYFSSENMDGSNPALNVLQRLCAATEQLNLQVDGGAECLVEIRSIVSESDVSSFEIQHSGFVKQLLLYLTSKSEKDAVSREIRLKRFLHVFFSSPLPGEEPIGRVEPVGNAPLLALVHKMNNCLSQMEQFPVKVHDFPSGNGTGGSFSLNRGSQALKFFNTHQLKCQLQRHPDCANVKQWKGGPVKIDPLALVQAIERYLVVRGYGRVREDDEDSDDDGSDEEIDESLAAQFLNSGNVRHRLQFYIGEHLLPYNMTVYQAVRQFSIQAEDERESTDDESNPLGRAGIWTKTHTIWYKPVREDEESNKDCVGGKRGRAQTAPTKTSPRNAKKHDELWHDGVCPSVSNPLEVYLISSPPENITFEDPSLDVILLLRVLHAISRYWYYLYDNAMCKEIIPTSEFINSKLTAKANRQLQDPLVIMTGNIPTWLTELGKTCPFFFPFDTRQMLFYVTAFDRDRAMQRLLDTNPEINQSDSQDSRVAPRLDRKKRTVNREELLKQAESVMQDLGSSRAMLEIQYENEVGTGLGPTLEFYALVSQELQRADLGLWRGEEVTLSNPKGSQEGTKYIQNLQGLFALPFGRTAKPAHIAKVKMKFRFLGKLMAKAIMDFRLVDLPLGLPFYKWMLRQETSLTSHDLFDIDPVVARSVYHLEDIVRQKKRLEQDKSQTKESLQYALETLTMNGCSVEDLGLDFTLPGFPNIELKKGGKDIPVTIHNLEEYLRLVIFWALNEGVSRQFDSFRDGFESVFPLSHLQYFYPEELDQLLCGSKADTWDAKTLMECCRPDHGYTHDSRAVKFLFEILSSFDNEQQRLFLQFVTGSPRLPVGGFRSLNPPLTIVRKTFESTENPDDFLPSVMTCVNYLKLPDYSSIDIMREKLLIAAREGQQSFHLS

Target ORF information:

RefSeq Version	XM_022409676.1
Organism	Canis lupus familiaris(dog)
Definition	Canis lupus familiaris thyroid hormone receptor interactor 12 (TRIP12), transcript variant X16, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_022409676.1

ORF Insert Sequence:

ATGTCCAACC GGCCTAATAA CAATCCAGGG GGGTCACTGC GACGTTCACA GAGGAACACT 
GCCGGGGCCC AACCACAAGA CGACTCGATA GGAGGAAGAA GCTGCAGTTC ATCATCTGCT 
GTCATAGTTC CACAACCAGA GGATCCAGAC AGAGCCAATA CTTCAGAAAG GCAAAAAACG 
GGGCAGGTGC CTAAGAAAGA CAATTCTCGA GGAGTGAAAC GCAGTGCTAG TCCAGACTAC 
AACAGGACCA ATTCTCCTAG CTCTGCCAAA AAACCAAAAG CACTTCAGCA TACTGAATCT 
CCCTCAGAAA CAAATAAGCC ACATAGTAAG TCGAAGAAGA GACATTTAGA CCAGGAGCAA 
CAACTGAAAT CTGCACAGTC GCCATCAACA AGTAAGGCTC ACACCAGAAA GAGTGGGGCC 
ACCGGCGGCT CACGGAGTCA GAAAAGAAAG AGGACAGAGA GTTCTTGTCT AAAGAGTGGT 
TCTGTGTCTG AGTCAACTGG TGCCGAAGAG AGATCTGCAA AACCCACCAA GCTGGCTTCA 
AAATCCGCCG CCTCGGCCAA AGCTGGGTGC AGCACCATCA CTGATTCTTC CTCCGCTGCC 
TCTACTTCCT CCTCATCTTC TGCTGTCGCC TCGGCCTCCT CCACTGTACC ACCAGGGGCC 
AGGGTGAAAC AAGGAAAAGA CCAGAGCAAG GCCAGGCGTT CCCGTTCAGC ATCCAGTCCC 
AGTCCCAGAA GAAGTAGCAG GGAAAAGGAA CAGAGTAAAA CTGGTGGCTC TTCAAAATTC 
GATTGGGCTG CTCGTTTCAG CCCTAAAGTT AGCCTTCCTA AAACCAAACT GTCTCTTCCA 
GGGTCTTCTA AGTCAGAGAC ATCAAAACCT GGACCTTCTG GATTACAGGC CAAATTAGCA 
AGTTTAAGAA AATCTACCAA GAAGCGCAGT GAATCCCCAC CTGCTGAGCT CCCCAGTTTG 
AGGCGGAGCA CACGCCAAAA GACCACGGGC TCCTGTGCTA GTGCCAGTCG GCGAGGCTCT 
GGCCTGGGCA AAAGAGGAGC AGCTGAAGCT CGTCGACAGG AGAAGATGGC GGACCCTGAA 
AGCAGCCAGG AGACCGTGAA TTCTTCAGCT GCACGGACAG ACGAAACTCC CCAAGGAGCT 
GCAGCCTCTA GTTCTGTTGC AGGGGCTGTG GGCATGACCA CCTCTGGGGA GAGTGAATCC 
GATGATTCTG AGATGGGACG ACTACAAGCT TTGTTAGAGG CCAGGGGTCT TCCTCCTCAT 
CTGTTTGGCC CTCTCGGTCC TCGGATGTCA CAACTTTTCC ATAGAACAAT TGGAAGCGGA 
GCTAGCTCTA AGGCCCAGCA GCTTCTGCAA GGACTACAAG CAAGTGATGA AAGCCAGCAA 
CTCCAGGCAG TTATTGAAAT GTGTCAGTTA TTGGTTATGG GAAATGAGGA GACGCTGGGA 
GGGTTTCCGG TCAAGAGTGT CGTTCCAGCT TTGATAACAT TACTGCAGAT GGAGCATAAT 
TTCGATATTA TGAACCATGC TTGTCGAGCC TTAACATATA TGATGGAAGC ACTTCCTCGG 
TCATCTGCTG TTGTAGTAGA TGCAATTCCT GTCTTTTTAG AAAAGCTGCA AGTTATTCAG 
TGTATTGATG TCGCAGAGCA GGCCCTGACT GCCTTGGAGA TGTTATCACG CAGACACAGT 
AAAGCCATTC TACAGGCGGG TGGTTTGGCA GACTGCTTGC TGTATCTAGA ATTCTTCAGC 
ATAAATGCCC AAAGAAATGC ACTAGCAATT GCAGCCAATT GCTGCCAGAG TATCACGCCA 
GATGAATTTC ATTTTGTGGC AGATTCCCTC CCGTTGCTTA CCCAAAGGCT AACCCATCAG 
GACAAAAAGT CAGTTGAAAG CACTTGCCTT TGTTTTGCAC GTCTAGTGGA CAACTTCCAA 
CATGAGGAGA ATTTACTCCA GCAGGTTGCC TCCAAAGATC TGCTTACGAA TGTTCAACAA 
CTCTTGGTAG TGACTCCACC CATTTTAAGT TCTGGGATGT TTATAATGGT GGTTCGCATG 
TTTTCTCTGA TGTGTTCCAA CTGTCCAACT TTAGCTGTTC AACTTATGAA GCAAAACATT 
GCAGAAACAC TTCACTTTCT CCTGTGTGGT GCCTCTAATG GAAGTTGTCA GGAACAGATT 
GATCTTGTTC CACGAAGTCC ACAAGAACTG TATGAACTGA CATCTCTGAT TTGCGAACTT 
ATGCCATGTT TACCAAAAGA AGGCATTTTT GCAGTTGATA CCATGCTGAA GAAGGGAAAT 
GCGCAGAACA CAGACGGTGC GATTTGGCAG TGGCGTGATG ACCGTGGCCT TTGGCATCCC 
TATAACAGGA TTGACAGCCG GATCATTGAG CAAATCAATG AGGACACGGG AACAGCACGT 
GCCATTCAGA GAAAACCTAA CCCCTTAGCC AATACTAACA CTAGTGGATA TTCAGAGTTA 
AAGAAGGATG ATGCTCGAGC ACAGCTTATG AAAGAGGATC CGGAACTGGC TAAGTCTTTT 
ATTAAGACAT TATTTGGTGT TCTTTATGAA GTGTATAGTT CCTCAGCAGG ACCTGCGGTC 
AGACATAAGT GCCTTAGAGC AATTCTTAGG ATAATTTATT TTGCGGATGC TGAACTTCTG 
AAGGATGTCC TGAAAAATCA TGCTGTTTCA AGTCACATTG CTTCCATGCT GTCAAGCCAA 
GACCTGAAGA TAGTAGTTGG AGCACTTCAG ATGGCAGAAA TCTTAATGCA GAAGTTACCT 
GATATTTTTA GTGTTTACTT CAGAAGAGAA GGTGTAATGC ATCAAGTAAA ACACTTAGCA 
GAATCAGAGT CTTTGTTGAC AAGTCCACCA AAGGCATGTA CGAATGGGTC AGGATCCCTG 
GGATCCACGA CGTCAGTCAG CAGTGGAACA GCCACGGCCG CCACCAATGC TGCAGCTGAC 
CTGGGGTCCC CCAGCTTGCA GCACAGCAGA GATGACTCTT TAGATCTGAG CCCTCAAGGT 
CGATTAAGTG ATGTTCTAAA GAGAAAACGG CTGCCAAAAC GAGGGCCAAG AAGGCCAAAG 
TATTCACCTC CAAGAGATGA TGACAAAGTA GACAATCAAG CTAAAAGCCC TACCACTACT 
CAGTCACCTA AATCTTCTTT CCTGGCAAGC TTGAATCCAA AAACGTGGGG AAGGTTAAGC 
GCACAGTCCA ACAGCAACAA CATCGAACCA GCACGAACTG CGGGAGTCAG TGGTCTTGCC 
AGGGCCGCCT CCAAGGACAC CATATCCAAT AACAGAGAAA AAATTAAAGG TTGGATTAAG 
GAACAGGCGC ATAAATTTGT AGAGCGTTAT TTCAGTTCTG AGAACATGGA TGGAAGCAAC 
CCTGCACTGA ATGTCCTTCA GAGACTTTGT GCTGCAACTG AACAACTCAA CCTCCAGGTG 
GATGGTGGAG CTGAGTGCCT TGTAGAAATC CGTAGCATAG TCTCAGAGTC TGATGTTTCA 
TCATTTGAAA TCCAACATAG TGGATTTGTG AAGCAGCTGT TACTTTATTT GACATCTAAA 
AGTGAAAAGG ATGCTGTGAG CCGAGAGATC AGATTAAAGA GATTCCTTCA CGTATTTTTT 
TCTTCACCAC TTCCTGGAGA AGAGCCCATT GGAAGAGTAG AACCAGTGGG TAATGCGCCT 
TTGTTGGCAT TAGTTCACAA GATGAACAAC TGCCTCAGCC AGATGGAACA GTTTCCAGTC 
AAAGTACATG ACTTCCCCAG TGGGAACGGG ACAGGAGGCA GCTTTTCTCT CAACAGAGGA 
TCACAGGCTT TAAAATTTTT CAACACACAC CAGTTAAAAT GCCAGTTACA AAGACATCCA 
GACTGTGCAA ATGTGAAGCA GTGGAAGGGC GGGCCGGTCA AGATTGATCC CCTGGCTTTG 
GTACAAGCTA TTGAGAGATA CCTTGTAGTT AGAGGGTATG GAAGAGTAAG AGAAGATGAT 
GAAGACAGTG ATGATGATGG CTCTGATGAG GAAATAGACG AGTCTCTGGC CGCTCAGTTC 
TTAAATTCCG GAAACGTAAG GCACAGGCTA CAGTTTTATA TTGGAGAACA TTTGCTACCA 
TATAACATGA CTGTATATCA GGCAGTCCGG CAGTTCAGTA TACAAGCTGA GGATGAGCGG 
GAGTCCACAG ATGATGAGAG CAACCCTCTG GGAAGAGCTG GTATTTGGAC AAAGACTCAT 
ACAATATGGT ACAAACCTGT GAGAGAGGAT GAAGAAAGTA ATAAAGATTG TGTTGGTGGT 
AAAAGAGGAA GAGCCCAAAC GGCTCCAACA AAAACATCCC CTAGAAATGC AAAAAAGCAT 
GATGAGTTAT GGCATGATGG AGTATGCCCA TCAGTATCCA ATCCTTTAGA AGTTTACCTC 
ATCTCCTCGC CACCTGAGAA TATAACTTTT GAAGACCCGT CCTTAGATGT GATTCTTCTT 
CTAAGAGTTT TACATGCCAT CAGCCGATAC TGGTATTACT TGTATGATAA TGCAATGTGC 
AAGGAGATTA TTCCAACTAG TGAATTTATT AACAGTAAGT TGACAGCAAA AGCAAATAGG 
CAGCTTCAAG ATCCTTTAGT AATCATGACA GGAAATATCC CAACGTGGCT TACTGAACTA 
GGAAAAACCT GCCCATTTTT CTTCCCTTTT GATACCCGGC AAATGCTTTT TTACGTAACT 
GCATTTGATC GGGACCGAGC AATGCAAAGA TTACTTGATA CCAACCCAGA AATCAACCAG 
TCGGATTCTC AAGATAGCAG AGTTGCACCT AGATTGGATA GAAAAAAACG TACTGTGAAC 
AGAGAGGAGC TGCTGAAACA AGCGGAGTCT GTAATGCAGG ATCTCGGTAG CTCACGGGCC 
ATGTTAGAAA TCCAGTATGA AAATGAGGTT GGTACAGGTC TTGGGCCTAC ACTGGAGTTT 
TATGCACTTG TATCTCAGGA ACTTCAGAGA GCTGACTTGG GTCTCTGGAG AGGTGAAGAA 
GTGACTCTCA GCAATCCAAA AGGAAGCCAA GAAGGGACCA AGTATATTCA AAACCTCCAG 
GGCCTGTTTG CACTTCCCTT TGGTAGGACA GCTAAGCCAG CCCATATCGC AAAGGTTAAA 
ATGAAGTTTC GCTTCTTAGG AAAATTAATG GCCAAGGCTA TCATGGATTT CAGATTGGTG 
GACCTTCCCC TTGGTTTACC CTTTTATAAG TGGATGCTAA GGCAAGAAAC TTCACTGACG 
TCGCATGATT TGTTTGACAT TGACCCAGTT GTAGCCAGAT CAGTGTATCA CCTAGAAGAC 
ATTGTCAGAC AGAAGAAAAG ACTTGAGCAA GATAAATCCC AGACCAAAGA GAGTCTACAA 
TATGCATTAG AAACCCTGAC TATGAATGGC TGCTCAGTTG AAGATCTAGG ATTGGATTTC 
ACGCTGCCAG GGTTTCCCAA CATTGAACTG AAGAAAGGAG GAAAGGATAT ACCAGTTACC 
ATCCACAATT TAGAGGAGTA TCTAAGACTG GTTATATTCT GGGCACTAAA TGAAGGCGTT 
TCCAGGCAAT TTGATTCCTT CAGAGATGGA TTTGAATCTG TCTTCCCACT TAGTCATCTT 
CAGTACTTCT ACCCGGAAGA ACTGGATCAG CTCTTGTGCG GCAGTAAAGC AGACACGTGG 
GATGCGAAGA CACTGATGGA GTGCTGCAGG CCGGACCACG GTTATACTCA TGACAGTCGG 
GCTGTGAAGT TCTTGTTTGA GATTCTCAGT AGTTTTGATA ATGAGCAGCA GAGGTTGTTT 
CTCCAGTTTG TGACAGGTAG CCCAAGATTG CCTGTTGGAG GCTTCCGGAG TCTGAATCCA 
CCTCTGACAA TTGTCCGGAA GACCTTCGAG TCCACGGAGA ACCCCGACGA CTTCTTGCCC 
TCTGTAATGA CTTGTGTGAA CTACCTTAAG TTGCCGGACT ACTCGAGCAT TGACATCATG 
CGTGAGAAAC TGTTGATTGC AGCGAGAGAA GGGCAGCAGT CATTCCATCT TTCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TRIP12 ( XM_022409669.1 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_022265377.1 Canis lupus familiaris thyroid hormone receptor interactor 12 (TRIP12), transcript variant X9, mRNA.

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CloneID	OCe25766
Clone ID Related Accession (Same CDS sequence)	XM_022409669.1
Accession Version	XM_022409669.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6120bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-09-04
Organism	Canis lupus familiaris(dog)
Product	E3 ubiquitin-protein ligase TRIP12 isoform X7
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006607.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCCAACC GGCCTAATAA CAATCCAGGG GGGTCACTGC GACGTTCACA GAGGAACACT 
GCCGGGGCCC AACCACAAGA CGACTCGATA GGAGGAAGGT CACATTTAGG GCAGGCAAAA 
CATAAGGGAT ATAGCCCTCC TGAGAGTAGA AAATCTAATT CTAAGGCACC CAAAGTGCAG 
TCTAATACTA CTTCTGAACT GTCAAGGGGA CACCTTTCTA AAAGAAGCTG CAGTTCATCA 
TCTGCTGTCA TAGTTCCACA ACCAGAGGAT CCAGACAGAG CCAATACTTC AGAAAGGCAA 
AAAACGGGGC AGGTGCCTAA GAAAGACAAT TCTCGAGGAG TGAAACGCAG TGCTAGTCCA 
GACTACAACA GGACCAATTC TCCTAGCTCT GCCAAAAAAC CAAAAGCACT TCAGCATACT 
GAATCTCCCT CAGAAACAAA TAAGCCACAT AGTAAGTCGA AGAAGAGACA TTTAGACCAG 
GAGCAACAAC TGAAATCTGC ACAGTCGCCA TCAACAAGTA AGGCTCACAC CAGAAAGAGT 
GGGGCCACCG GCGGCTCACG GAGTCAGAAA AGAAAGAGGA CAGAGAGTTC TTGTCTAAAG 
AGTGGTTCTG TGTCTGAGTC AACTGGTGCC GAAGAGAGAT CTGCAAAACC CACCAAGCTG 
GCTTCAAAAT CCGCCGCCTC GGCCAAAGCT GGGTGCAGCA CCATCACTGA TTCTTCCTCC 
GCTGCCTCTA CTTCCTCCTC ATCTTCTGCT GTCGCCTCGG CCTCCTCCAC TGTACCACCA 
GGGGCCAGGG TGAAACAAGG AAAAGACCAG AGCAAGGCCA GGCGTTCCCG TTCAGCATCC 
AGTCCCAGTC CCAGAAGAAG TAGCAGGGAA AAGGAACAGA GGTCTTCTAA GTCAGAGACA 
TCAAAACCTG GACCTTCTGG ATTACAGGCC AAATTAGCAA GTTTAAGAAA ATCTACCAAG 
AAGCGCAGTG AATCCCCACC TGCTGAGCTC CCCAGTTTGA GGCGGAGCAC ACGCCAAAAG 
ACCACGGGCT CCTGTGCTAG TGCCAGTCGG CGAGGCTCTG GCCTGGGCAA AAGAGGAGCA 
GCTGAAGCTC GTCGACAGGA GAAGATGGCG GACCCTGAAA GCAGCCAGGA GACCGTGAAT 
TCTTCAGCTG CACGGACAGA CGAAACTCCC CAAGGAGCTG CAGCCTCTAG TTCTGTTGCA 
GGGGCTGTGG GCATGACCAC CTCTGGGGAG AGTGAATCCG ATGATTCTGA GATGGGACGA 
CTACAAGCTT TGTTAGAGGC CAGGGGTCTT CCTCCTCATC TGTTTGGCCC TCTCGGTCCT 
CGGATGTCAC AACTTTTCCA TAGAACAATT GGAAGCGGAG CTAGCTCTAA GGCCCAGCAG 
CTTCTGCAAG GACTACAAGC AAGTGATGAA AGCCAGCAAC TCCAGGCAGT TATTGAAATG 
TGTCAGTTAT TGGTTATGGG AAATGAGGAG ACGCTGGGAG GGTTTCCGGT CAAGAGTGTC 
GTTCCAGCTT TGATAACATT ACTGCAGATG GAGCATAATT TCGATATTAT GAACCATGCT 
TGTCGAGCCT TAACATATAT GATGGAAGCA CTTCCTCGGT CATCTGCTGT TGTAGTAGAT 
GCAATTCCTG TCTTTTTAGA AAAGCTGCAA GTTATTCAGT GTATTGATGT CGCAGAGCAG 
GCCCTGACTG CCTTGGAGAT GTTATCACGC AGACACAGTA AAGCCATTCT ACAGGCGGGT 
GGTTTGGCAG ACTGCTTGCT GTATCTAGAA TTCTTCAGCA TAAATGCCCA AAGAAATGCA 
CTAGCAATTG CAGCCAATTG CTGCCAGAGT ATCACGCCAG ATGAATTTCA TTTTGTGGCA 
GATTCCCTCC CGTTGCTTAC CCAAAGGCTA ACCCATCAGG ACAAAAAGTC AGTTGAAAGC 
ACTTGCCTTT GTTTTGCACG TCTAGTGGAC AACTTCCAAC ATGAGGAGAA TTTACTCCAG 
CAGGTTGCCT CCAAAGATCT GCTTACGAAT GTTCAACAAC TCTTGGTAGT GACTCCACCC 
ATTTTAAGTT CTGGGATGTT TATAATGGTG GTTCGCATGT TTTCTCTGAT GTGTTCCAAC 
TGTCCAACTT TAGCTGTTCA ACTTATGAAG CAAAACATTG CAGAAACACT TCACTTTCTC 
CTGTGTGGTG CCTCTAATGG AAGTTGTCAG GAACAGATTG ATCTTGTTCC ACGAAGTCCA 
CAAGAACTGT ATGAACTGAC ATCTCTGATT TGCGAACTTA TGCCATGTTT ACCAAAAGAA 
GGCATTTTTG CAGTTGATAC CATGCTGAAG AAGGGAAATG CGCAGAACAC AGACGGTGCG 
ATTTGGCAGT GGCGTGATGA CCGTGGCCTT TGGCATCCCT ATAACAGGAT TGACAGCCGG 
ATCATTGAGG TAGCAGCCCA TCAGGTCGGT GAGGATGAGA TAAGCTTGTC CACTCTGGGA 
CGAGTTTATA CTATTGATTT TAATTCTATG CAGCAAATCA ATGAGGACAC GGGAACAGCA 
CGTGCCATTC AGAGAAAACC TAACCCCTTA GCCAATACTA ACACTAGTGG ATATTCAGAG 
TTAAAGAAGG ATGATGCTCG AGCACAGCTT ATGAAAGAGG ATCCGGAACT GGCTAAGTCT 
TTTATTAAGA CATTATTTGG TGTTCTTTAT GAAGTGTATA GTTCCTCAGC AGGACCTGCG 
GTCAGACATA AGTGCCTTAG AGCAATTCTT AGGATAATTT ATTTTGCGGA TGCTGAACTT 
CTGAAGGATG TCCTGAAAAA TCATGCTGTT TCAAGTCACA TTGCTTCCAT GCTGTCAAGC 
CAAGACCTGA AGATAGTAGT TGGAGCACTT CAGATGGCAG AAATCTTAAT GCAGAAGTTA 
CCTGATATTT TTAGTGTTTA CTTCAGAAGA GAAGGTGTAA TGCATCAAGT AAAACACTTA 
GCAGAATCAG AGTCTTTGTT GACAAGTCCA CCAAAGGCAT GTACGAATGG GTCAGGATCC 
CTGGGATCCA CGACGTCAGT CAGCAGTGGA ACAGCCACGG CCGCCACCAA TGCTGCAGCT 
GACCTGGGGT CCCCCAGCTT GCAGCACAGC AGAGATGACT CTTTAGATCT GAGCCCTCAA 
GGTCGATTAA GTGATGTTCT AAAGAGAAAA CGGCTGCCAA AACGAGGGCC AAGAAGGCCA 
AAGTATTCAC CTCCAAGAGA TGATGACAAA GTAGACAATC AAGCTAAAAG CCCTACCACT 
ACTCAGTCAC CTAAATCTTC TTTCCTGGCA AGCTTGAATC CAAAAACGTG GGGAAGGTTA 
AGCGCACAGT CCAACAGCAA CAACATCGAA CCAGCACGAA CTGCGGGAGT CAGTGGTCTT 
GCCAGGGCCG CCTCCAAGGA CACCATATCC AATAACAGAG AAAAAATTAA AGGTTGGATT 
AAGGAACAGG CGCATAAATT TGTAGAGCGT TATTTCAGTT CTGAGAACAT GGATGGAAGC 
AACCCTGCAC TGAATGTCCT TCAGAGACTT TGTGCTGCAA CTGAACAACT CAACCTCCAG 
GTGGATGGTG GAGCTGAGTG CCTTGTAGAA ATCCGTAGCA TAGTCTCAGA GTCTGATGTT 
TCATCATTTG AAATCCAACA TAGTGGATTT GTGAAGCAGC TGTTACTTTA TTTGACATCT 
AAAAGTGAAA AGGATGCTGT GAGCCGAGAG ATCAGATTAA AGAGATTCCT TCACGTATTT 
TTTTCTTCAC CACTTCCTGG AGAAGAGCCC ATTGGAAGAG TAGAACCAGT GGGTAATGCG 
CCTTTGTTGG CATTAGTTCA CAAGATGAAC AACTGCCTCA GCCAGATGGA ACAGTTTCCA 
GTCAAAGTAC ATGACTTCCC CAGTGGGAAC GGGACAGGAG GCAGCTTTTC TCTCAACAGA 
GGATCACAGG CTTTAAAATT TTTCAACACA CACCAGTTAA AATGCCAGTT ACAAAGACAT 
CCAGACTGTG CAAATGTGAA GCAGTGGAAG GGCGGGCCGG TCAAGATTGA TCCCCTGGCT 
TTGGTACAAG CTATTGAGAG ATACCTTGTA GTTAGAGGGT ATGGAAGAGT AAGAGAAGAT 
GATGAAGACA GTGATGATGA TGGCTCTGAT GAGGAAATAG ACGAGTCTCT GGCCGCTCAG 
TTCTTAAATT CCGGAAACGT AAGGCACAGG CTACAGTTTT ATATTGGAGA ACATTTGCTA 
CCATATAACA TGACTGTATA TCAGGCAGTC CGGCAGTTCA GTATACAAGC TGAGGATGAG 
CGGGAGTCCA CAGATGATGA GAGCAACCCT CTGGGAAGAG CTGGTATTTG GACAAAGACT 
CATACAATAT GGTACAAACC TGTGAGAGAG GATGAAGAAA GTAATAAAGA TTGTGTTGGT 
GGTAAAAGAG GAAGAGCCCA AACGGCTCCA ACAAAAACAT CCCCTAGAAA TGCAAAAAAG 
CATGATGAGT TATGGCATGA TGGAGTATGC CCATCAGTAT CCAATCCTTT AGAAGTTTAC 
CTCATCTCCT CGCCACCTGA GAATATAACT TTTGAAGACC CGTCCTTAGA TGTGATTCTT 
CTTCTAAGAG TTTTACATGC CATCAGCCGA TACTGGTATT ACTTGTATGA TAATGCAATG 
TGCAAGGAGA TTATTCCAAC TAGTGAATTT ATTAACAGTA AGTTGACAGC AAAAGCAAAT 
AGGCAGCTTC AAGATCCTTT AGTAATCATG ACAGGAAATA TCCCAACGTG GCTTACTGAA 
CTAGGAAAAA CCTGCCCATT TTTCTTCCCT TTTGATACCC GGCAAATGCT TTTTTACGTA 
ACTGCATTTG ATCGGGACCG AGCAATGCAA AGATTACTTG ATACCAACCC AGAAATCAAC 
CAGTCGGATT CTCAAGATAG CAGAGTTGCA CCTAGATTGG ATAGAAAAAA ACGTACTGTG 
AACAGAGAGG AGCTGCTGAA ACAAGCGGAG TCTGTAATGC AGGATCTCGG TAGCTCACGG 
GCCATGTTAG AAATCCAGTA TGAAAATGAG GTTGGTACAG GTCTTGGGCC TACACTGGAG 
TTTTATGCAC TTGTATCTCA GGAACTTCAG AGAGCTGACT TGGGTCTCTG GAGAGGTGAA 
GAAGTGACTC TCAGCAATCC AAAAGGAAGC CAAGAAGGGA CCAAGTATAT TCAAAACCTC 
CAGGGCCTGT TTGCACTTCC CTTTGGTAGG ACAGCTAAGC CAGCCCATAT CGCAAAGGTT 
AAAATGAAGT TTCGCTTCTT AGGAAAATTA ATGGCCAAGG CTATCATGGA TTTCAGATTG 
GTGGACCTTC CCCTTGGTTT ACCCTTTTAT AAGTGGATGC TAAGGCAAGA AACTTCACTG 
ACGTCGCATG ATTTGTTTGA CATTGACCCA GTTGTAGCCA GATCAGTGTA TCACCTAGAA 
GACATTGTCA GACAGAAGAA AAGACTTGAG CAAGATAAAT CCCAGACCAA AGAGAGTCTA 
CAATATGCAT TAGAAACCCT GACTATGAAT GGCTGCTCAG TTGAAGATCT AGGATTGGAT 
TTCACGCTGC CAGGGTTTCC CAACATTGAA CTGAAGAAAG GAGGAAAGGA TATACCAGTT 
ACCATCCACA ATTTAGAGGA GTATCTAAGA CTGGTTATAT TCTGGGCACT AAATGAAGGC 
GTTTCCAGGC AATTTGATTC CTTCAGAGAT GGATTTGAAT CTGTCTTCCC ACTTAGTCAT 
CTTCAGTACT TCTACCCGGA AGAACTGGAT CAGCTCTTGT GCGGCAGTAA AGCAGACACG 
TGGGATGCGA AGACACTGAT GGAGTGCTGC AGGCCGGACC ACGGTTATAC TCATGACAGT 
CGGGCTGTGA AGTTCTTGTT TGAGATTCTC AGTAGTTTTG ATAATGAGCA GCAGAGGTTG 
TTTCTCCAGT TTGTGACAGG TAGCCCAAGA TTGCCTGTTG GAGGCTTCCG GAGTCTGAAT 
CCACCTCTGA CAATTGTCCG GAAGACCTTC GAGTCCACGG AGAACCCCGA CGACTTCTTG 
CCCTCTGTAA TGACTTGTGT GAACTACCTT AAGTTGCCGG ACTACTCGAG CATTGACATC 
ATGCGTGAGA AACTGTTGAT TGCAGCGAGA GAAGGGCAGC AGTCATTCCA TCTTTCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_022265377.1
CDS	161..6280
Translation	MSNRPNNNPGGSLRRSQRNTAGAQPQDDSIGGRSHLGQAKHKGYSPPESRKSNSKAPKVQSNTTSELSRGHLSKRSCSSSSAVIVPQPEDPDRANTSERQKTGQVPKKDNSRGVKRSASPDYNRTNSPSSAKKPKALQHTESPSETNKPHSKSKKRHLDQEQQLKSAQSPSTSKAHTRKSGATGGSRSQKRKRTESSCLKSGSVSESTGAEERSAKPTKLASKSAASAKAGCSTITDSSSAASTSSSSSAVASASSTVPPGARVKQGKDQSKARRSRSASSPSPRRSSREKEQRSSKSETSKPGPSGLQAKLASLRKSTKKRSESPPAELPSLRRSTRQKTTGSCASASRRGSGLGKRGAAEARRQEKMADPESSQETVNSSAARTDETPQGAAASSSVAGAVGMTTSGESESDDSEMGRLQALLEARGLPPHLFGPLGPRMSQLFHRTIGSGASSKAQQLLQGLQASDESQQLQAVIEMCQLLVMGNEETLGGFPVKSVVPALITLLQMEHNFDIMNHACRALTYMMEALPRSSAVVVDAIPVFLEKLQVIQCIDVAEQALTALEMLSRRHSKAILQAGGLADCLLYLEFFSINAQRNALAIAANCCQSITPDEFHFVADSLPLLTQRLTHQDKKSVESTCLCFARLVDNFQHEENLLQQVASKDLLTNVQQLLVVTPPILSSGMFIMVVRMFSLMCSNCPTLAVQLMKQNIAETLHFLLCGASNGSCQEQIDLVPRSPQELYELTSLICELMPCLPKEGIFAVDTMLKKGNAQNTDGAIWQWRDDRGLWHPYNRIDSRIIEVAAHQVGEDEISLSTLGRVYTIDFNSMQQINEDTGTARAIQRKPNPLANTNTSGYSELKKDDARAQLMKEDPELAKSFIKTLFGVLYEVYSSSAGPAVRHKCLRAILRIIYFADAELLKDVLKNHAVSSHIASMLSSQDLKIVVGALQMAEILMQKLPDIFSVYFRREGVMHQVKHLAESESLLTSPPKACTNGSGSLGSTTSVSSGTATAATNAAADLGSPSLQHSRDDSLDLSPQGRLSDVLKRKRLPKRGPRRPKYSPPRDDDKVDNQAKSPTTTQSPKSSFLASLNPKTWGRLSAQSNSNNIEPARTAGVSGLARAASKDTISNNREKIKGWIKEQAHKFVERYFSSENMDGSNPALNVLQRLCAATEQLNLQVDGGAECLVEIRSIVSESDVSSFEIQHSGFVKQLLLYLTSKSEKDAVSREIRLKRFLHVFFSSPLPGEEPIGRVEPVGNAPLLALVHKMNNCLSQMEQFPVKVHDFPSGNGTGGSFSLNRGSQALKFFNTHQLKCQLQRHPDCANVKQWKGGPVKIDPLALVQAIERYLVVRGYGRVREDDEDSDDDGSDEEIDESLAAQFLNSGNVRHRLQFYIGEHLLPYNMTVYQAVRQFSIQAEDERESTDDESNPLGRAGIWTKTHTIWYKPVREDEESNKDCVGGKRGRAQTAPTKTSPRNAKKHDELWHDGVCPSVSNPLEVYLISSPPENITFEDPSLDVILLLRVLHAISRYWYYLYDNAMCKEIIPTSEFINSKLTAKANRQLQDPLVIMTGNIPTWLTELGKTCPFFFPFDTRQMLFYVTAFDRDRAMQRLLDTNPEINQSDSQDSRVAPRLDRKKRTVNREELLKQAESVMQDLGSSRAMLEIQYENEVGTGLGPTLEFYALVSQELQRADLGLWRGEEVTLSNPKGSQEGTKYIQNLQGLFALPFGRTAKPAHIAKVKMKFRFLGKLMAKAIMDFRLVDLPLGLPFYKWMLRQETSLTSHDLFDIDPVVARSVYHLEDIVRQKKRLEQDKSQTKESLQYALETLTMNGCSVEDLGLDFTLPGFPNIELKKGGKDIPVTIHNLEEYLRLVIFWALNEGVSRQFDSFRDGFESVFPLSHLQYFYPEELDQLLCGSKADTWDAKTLMECCRPDHGYTHDSRAVKFLFEILSSFDNEQQRLFLQFVTGSPRLPVGGFRSLNPPLTIVRKTFESTENPDDFLPSVMTCVNYLKLPDYSSIDIMREKLLIAAREGQQSFHLS

Target ORF information:

RefSeq Version	XM_022409669.1
Organism	Canis lupus familiaris(dog)
Definition	Canis lupus familiaris thyroid hormone receptor interactor 12 (TRIP12), transcript variant X9, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_022409669.1

ORF Insert Sequence:

ATGTCCAACC GGCCTAATAA CAATCCAGGG GGGTCACTGC GACGTTCACA GAGGAACACT 
GCCGGGGCCC AACCACAAGA CGACTCGATA GGAGGAAGGT CACATTTAGG GCAGGCAAAA 
CATAAGGGAT ATAGCCCTCC TGAGAGTAGA AAATCTAATT CTAAGGCACC CAAAGTGCAG 
TCTAATACTA CTTCTGAACT GTCAAGGGGA CACCTTTCTA AAAGAAGCTG CAGTTCATCA 
TCTGCTGTCA TAGTTCCACA ACCAGAGGAT CCAGACAGAG CCAATACTTC AGAAAGGCAA 
AAAACGGGGC AGGTGCCTAA GAAAGACAAT TCTCGAGGAG TGAAACGCAG TGCTAGTCCA 
GACTACAACA GGACCAATTC TCCTAGCTCT GCCAAAAAAC CAAAAGCACT TCAGCATACT 
GAATCTCCCT CAGAAACAAA TAAGCCACAT AGTAAGTCGA AGAAGAGACA TTTAGACCAG 
GAGCAACAAC TGAAATCTGC ACAGTCGCCA TCAACAAGTA AGGCTCACAC CAGAAAGAGT 
GGGGCCACCG GCGGCTCACG GAGTCAGAAA AGAAAGAGGA CAGAGAGTTC TTGTCTAAAG 
AGTGGTTCTG TGTCTGAGTC AACTGGTGCC GAAGAGAGAT CTGCAAAACC CACCAAGCTG 
GCTTCAAAAT CCGCCGCCTC GGCCAAAGCT GGGTGCAGCA CCATCACTGA TTCTTCCTCC 
GCTGCCTCTA CTTCCTCCTC ATCTTCTGCT GTCGCCTCGG CCTCCTCCAC TGTACCACCA 
GGGGCCAGGG TGAAACAAGG AAAAGACCAG AGCAAGGCCA GGCGTTCCCG TTCAGCATCC 
AGTCCCAGTC CCAGAAGAAG TAGCAGGGAA AAGGAACAGA GGTCTTCTAA GTCAGAGACA 
TCAAAACCTG GACCTTCTGG ATTACAGGCC AAATTAGCAA GTTTAAGAAA ATCTACCAAG 
AAGCGCAGTG AATCCCCACC TGCTGAGCTC CCCAGTTTGA GGCGGAGCAC ACGCCAAAAG 
ACCACGGGCT CCTGTGCTAG TGCCAGTCGG CGAGGCTCTG GCCTGGGCAA AAGAGGAGCA 
GCTGAAGCTC GTCGACAGGA GAAGATGGCG GACCCTGAAA GCAGCCAGGA GACCGTGAAT 
TCTTCAGCTG CACGGACAGA CGAAACTCCC CAAGGAGCTG CAGCCTCTAG TTCTGTTGCA 
GGGGCTGTGG GCATGACCAC CTCTGGGGAG AGTGAATCCG ATGATTCTGA GATGGGACGA 
CTACAAGCTT TGTTAGAGGC CAGGGGTCTT CCTCCTCATC TGTTTGGCCC TCTCGGTCCT 
CGGATGTCAC AACTTTTCCA TAGAACAATT GGAAGCGGAG CTAGCTCTAA GGCCCAGCAG 
CTTCTGCAAG GACTACAAGC AAGTGATGAA AGCCAGCAAC TCCAGGCAGT TATTGAAATG 
TGTCAGTTAT TGGTTATGGG AAATGAGGAG ACGCTGGGAG GGTTTCCGGT CAAGAGTGTC 
GTTCCAGCTT TGATAACATT ACTGCAGATG GAGCATAATT TCGATATTAT GAACCATGCT 
TGTCGAGCCT TAACATATAT GATGGAAGCA CTTCCTCGGT CATCTGCTGT TGTAGTAGAT 
GCAATTCCTG TCTTTTTAGA AAAGCTGCAA GTTATTCAGT GTATTGATGT CGCAGAGCAG 
GCCCTGACTG CCTTGGAGAT GTTATCACGC AGACACAGTA AAGCCATTCT ACAGGCGGGT 
GGTTTGGCAG ACTGCTTGCT GTATCTAGAA TTCTTCAGCA TAAATGCCCA AAGAAATGCA 
CTAGCAATTG CAGCCAATTG CTGCCAGAGT ATCACGCCAG ATGAATTTCA TTTTGTGGCA 
GATTCCCTCC CGTTGCTTAC CCAAAGGCTA ACCCATCAGG ACAAAAAGTC AGTTGAAAGC 
ACTTGCCTTT GTTTTGCACG TCTAGTGGAC AACTTCCAAC ATGAGGAGAA TTTACTCCAG 
CAGGTTGCCT CCAAAGATCT GCTTACGAAT GTTCAACAAC TCTTGGTAGT GACTCCACCC 
ATTTTAAGTT CTGGGATGTT TATAATGGTG GTTCGCATGT TTTCTCTGAT GTGTTCCAAC 
TGTCCAACTT TAGCTGTTCA ACTTATGAAG CAAAACATTG CAGAAACACT TCACTTTCTC 
CTGTGTGGTG CCTCTAATGG AAGTTGTCAG GAACAGATTG ATCTTGTTCC ACGAAGTCCA 
CAAGAACTGT ATGAACTGAC ATCTCTGATT TGCGAACTTA TGCCATGTTT ACCAAAAGAA 
GGCATTTTTG CAGTTGATAC CATGCTGAAG AAGGGAAATG CGCAGAACAC AGACGGTGCG 
ATTTGGCAGT GGCGTGATGA CCGTGGCCTT TGGCATCCCT ATAACAGGAT TGACAGCCGG 
ATCATTGAGG TAGCAGCCCA TCAGGTCGGT GAGGATGAGA TAAGCTTGTC CACTCTGGGA 
CGAGTTTATA CTATTGATTT TAATTCTATG CAGCAAATCA ATGAGGACAC GGGAACAGCA 
CGTGCCATTC AGAGAAAACC TAACCCCTTA GCCAATACTA ACACTAGTGG ATATTCAGAG 
TTAAAGAAGG ATGATGCTCG AGCACAGCTT ATGAAAGAGG ATCCGGAACT GGCTAAGTCT 
TTTATTAAGA CATTATTTGG TGTTCTTTAT GAAGTGTATA GTTCCTCAGC AGGACCTGCG 
GTCAGACATA AGTGCCTTAG AGCAATTCTT AGGATAATTT ATTTTGCGGA TGCTGAACTT 
CTGAAGGATG TCCTGAAAAA TCATGCTGTT TCAAGTCACA TTGCTTCCAT GCTGTCAAGC 
CAAGACCTGA AGATAGTAGT TGGAGCACTT CAGATGGCAG AAATCTTAAT GCAGAAGTTA 
CCTGATATTT TTAGTGTTTA CTTCAGAAGA GAAGGTGTAA TGCATCAAGT AAAACACTTA 
GCAGAATCAG AGTCTTTGTT GACAAGTCCA CCAAAGGCAT GTACGAATGG GTCAGGATCC 
CTGGGATCCA CGACGTCAGT CAGCAGTGGA ACAGCCACGG CCGCCACCAA TGCTGCAGCT 
GACCTGGGGT CCCCCAGCTT GCAGCACAGC AGAGATGACT CTTTAGATCT GAGCCCTCAA 
GGTCGATTAA GTGATGTTCT AAAGAGAAAA CGGCTGCCAA AACGAGGGCC AAGAAGGCCA 
AAGTATTCAC CTCCAAGAGA TGATGACAAA GTAGACAATC AAGCTAAAAG CCCTACCACT 
ACTCAGTCAC CTAAATCTTC TTTCCTGGCA AGCTTGAATC CAAAAACGTG GGGAAGGTTA 
AGCGCACAGT CCAACAGCAA CAACATCGAA CCAGCACGAA CTGCGGGAGT CAGTGGTCTT 
GCCAGGGCCG CCTCCAAGGA CACCATATCC AATAACAGAG AAAAAATTAA AGGTTGGATT 
AAGGAACAGG CGCATAAATT TGTAGAGCGT TATTTCAGTT CTGAGAACAT GGATGGAAGC 
AACCCTGCAC TGAATGTCCT TCAGAGACTT TGTGCTGCAA CTGAACAACT CAACCTCCAG 
GTGGATGGTG GAGCTGAGTG CCTTGTAGAA ATCCGTAGCA TAGTCTCAGA GTCTGATGTT 
TCATCATTTG AAATCCAACA TAGTGGATTT GTGAAGCAGC TGTTACTTTA TTTGACATCT 
AAAAGTGAAA AGGATGCTGT GAGCCGAGAG ATCAGATTAA AGAGATTCCT TCACGTATTT 
TTTTCTTCAC CACTTCCTGG AGAAGAGCCC ATTGGAAGAG TAGAACCAGT GGGTAATGCG 
CCTTTGTTGG CATTAGTTCA CAAGATGAAC AACTGCCTCA GCCAGATGGA ACAGTTTCCA 
GTCAAAGTAC ATGACTTCCC CAGTGGGAAC GGGACAGGAG GCAGCTTTTC TCTCAACAGA 
GGATCACAGG CTTTAAAATT TTTCAACACA CACCAGTTAA AATGCCAGTT ACAAAGACAT 
CCAGACTGTG CAAATGTGAA GCAGTGGAAG GGCGGGCCGG TCAAGATTGA TCCCCTGGCT 
TTGGTACAAG CTATTGAGAG ATACCTTGTA GTTAGAGGGT ATGGAAGAGT AAGAGAAGAT 
GATGAAGACA GTGATGATGA TGGCTCTGAT GAGGAAATAG ACGAGTCTCT GGCCGCTCAG 
TTCTTAAATT CCGGAAACGT AAGGCACAGG CTACAGTTTT ATATTGGAGA ACATTTGCTA 
CCATATAACA TGACTGTATA TCAGGCAGTC CGGCAGTTCA GTATACAAGC TGAGGATGAG 
CGGGAGTCCA CAGATGATGA GAGCAACCCT CTGGGAAGAG CTGGTATTTG GACAAAGACT 
CATACAATAT GGTACAAACC TGTGAGAGAG GATGAAGAAA GTAATAAAGA TTGTGTTGGT 
GGTAAAAGAG GAAGAGCCCA AACGGCTCCA ACAAAAACAT CCCCTAGAAA TGCAAAAAAG 
CATGATGAGT TATGGCATGA TGGAGTATGC CCATCAGTAT CCAATCCTTT AGAAGTTTAC 
CTCATCTCCT CGCCACCTGA GAATATAACT TTTGAAGACC CGTCCTTAGA TGTGATTCTT 
CTTCTAAGAG TTTTACATGC CATCAGCCGA TACTGGTATT ACTTGTATGA TAATGCAATG 
TGCAAGGAGA TTATTCCAAC TAGTGAATTT ATTAACAGTA AGTTGACAGC AAAAGCAAAT 
AGGCAGCTTC AAGATCCTTT AGTAATCATG ACAGGAAATA TCCCAACGTG GCTTACTGAA 
CTAGGAAAAA CCTGCCCATT TTTCTTCCCT TTTGATACCC GGCAAATGCT TTTTTACGTA 
ACTGCATTTG ATCGGGACCG AGCAATGCAA AGATTACTTG ATACCAACCC AGAAATCAAC 
CAGTCGGATT CTCAAGATAG CAGAGTTGCA CCTAGATTGG ATAGAAAAAA ACGTACTGTG 
AACAGAGAGG AGCTGCTGAA ACAAGCGGAG TCTGTAATGC AGGATCTCGG TAGCTCACGG 
GCCATGTTAG AAATCCAGTA TGAAAATGAG GTTGGTACAG GTCTTGGGCC TACACTGGAG 
TTTTATGCAC TTGTATCTCA GGAACTTCAG AGAGCTGACT TGGGTCTCTG GAGAGGTGAA 
GAAGTGACTC TCAGCAATCC AAAAGGAAGC CAAGAAGGGA CCAAGTATAT TCAAAACCTC 
CAGGGCCTGT TTGCACTTCC CTTTGGTAGG ACAGCTAAGC CAGCCCATAT CGCAAAGGTT 
AAAATGAAGT TTCGCTTCTT AGGAAAATTA ATGGCCAAGG CTATCATGGA TTTCAGATTG 
GTGGACCTTC CCCTTGGTTT ACCCTTTTAT AAGTGGATGC TAAGGCAAGA AACTTCACTG 
ACGTCGCATG ATTTGTTTGA CATTGACCCA GTTGTAGCCA GATCAGTGTA TCACCTAGAA 
GACATTGTCA GACAGAAGAA AAGACTTGAG CAAGATAAAT CCCAGACCAA AGAGAGTCTA 
CAATATGCAT TAGAAACCCT GACTATGAAT GGCTGCTCAG TTGAAGATCT AGGATTGGAT 
TTCACGCTGC CAGGGTTTCC CAACATTGAA CTGAAGAAAG GAGGAAAGGA TATACCAGTT 
ACCATCCACA ATTTAGAGGA GTATCTAAGA CTGGTTATAT TCTGGGCACT AAATGAAGGC 
GTTTCCAGGC AATTTGATTC CTTCAGAGAT GGATTTGAAT CTGTCTTCCC ACTTAGTCAT 
CTTCAGTACT TCTACCCGGA AGAACTGGAT CAGCTCTTGT GCGGCAGTAA AGCAGACACG 
TGGGATGCGA AGACACTGAT GGAGTGCTGC AGGCCGGACC ACGGTTATAC TCATGACAGT 
CGGGCTGTGA AGTTCTTGTT TGAGATTCTC AGTAGTTTTG ATAATGAGCA GCAGAGGTTG 
TTTCTCCAGT TTGTGACAGG TAGCCCAAGA TTGCCTGTTG GAGGCTTCCG GAGTCTGAAT 
CCACCTCTGA CAATTGTCCG GAAGACCTTC GAGTCCACGG AGAACCCCGA CGACTTCTTG 
CCCTCTGTAA TGACTTGTGT GAACTACCTT AAGTTGCCGG ACTACTCGAG CATTGACATC 
ATGCGTGAGA AACTGTTGAT TGCAGCGAGA GAAGGGCAGC AGTCATTCCA TCTTTCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TRIP12 ( XM_022409666.1 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_022265374.1 Canis lupus familiaris thyroid hormone receptor interactor 12 (TRIP12), transcript variant X6, mRNA.

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CloneID	OCe297063
Clone ID Related Accession (Same CDS sequence)	XM_022409666.1
Accession Version	XM_022409666.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6189bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-09-04
Organism	Canis lupus familiaris(dog)
Product	E3 ubiquitin-protein ligase TRIP12 isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006607.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCCAACC GGCCTAATAA CAATCCAGGG GGGTCACTGC GACGTTCACA GAGGAACACT 
GCCGGGGCCC AACCACAAGA CGACTCGATA GGAGGAAGGT CACATTTAGG GCAGGCAAAA 
CATAAGGGAT ATAGCCCTCC TGAGAGTAGA AAATCTAATT CTAAGGCACC CAAAGTGCAG 
TCTAATACTA CTTCTGAACT GTCAAGGGGA CACCTTTCTA AAAGAAGCTG CAGTTCATCA 
TCTGCTGTCA TAGTTCCACA ACCAGAGGAT CCAGACAGAG CCAATACTTC AGAAAGGCAA 
AAAACGGGGC AGGTGCCTAA GAAAGACAAT TCTCGAGGAG TGAAACGCAG TGCTAGTCCA 
GACTACAACA GGACCAATTC TCCTAGCTCT GCCAAAAAAC CAAAAGCACT TCAGCATACT 
GAATCTCCCT CAGAAACAAA TAAGCCACAT AGTAAGTCGA AGAAGAGACA TTTAGACCAG 
GAGCAACAAC TGAAATCTGC ACAGTCGCCA TCAACAAGTA AGGCTCACAC CAGAAAGAGT 
GGGGCCACCG GCGGCTCACG GAGTCAGAAA AGAAAGAGGA CAGAGAGTTC TTGTCTAAAG 
AGTGGTTCTG TGTCTGAGTC AACTGGTGCC GAAGAGAGAT CTGCAAAACC CACCAAGCTG 
GCTTCAAAAT CCGCCGCCTC GGCCAAAGCT GGGTGCAGCA CCATCACTGA TTCTTCCTCC 
GCTGCCTCTA CTTCCTCCTC ATCTTCTGCT GTCGCCTCGG CCTCCTCCAC TGTACCACCA 
GGGGCCAGGG TGAAACAAGG AAAAGACCAG AGCAAGGCCA GGCGTTCCCG TTCAGCATCC 
AGTCCCAGTC CCAGAAGAAG TAGCAGGGAA AAGGAACAGA GTAAAACTGG TGGCTCTTCA 
AAATTCGATT GGGCTGCTCG TTTCAGCCCT AAAGTTAGCC TTCCTAAAAC CAAACTGTCT 
CTTCCAGGGT CTTCTAAGTC AGAGACATCA AAACCTGGAC CTTCTGGATT ACAGGCCAAA 
TTAGCAAGTT TAAGAAAATC TACCAAGAAG CGCAGTGAAT CCCCACCTGC TGAGCTCCCC 
AGTTTGAGGC GGAGCACACG CCAAAAGACC ACGGGCTCCT GTGCTAGTGC CAGTCGGCGA 
GGCTCTGGCC TGGGCAAAAG AGGAGCAGCT GAAGCTCGTC GACAGGAGAA GATGGCGGAC 
CCTGAAAGCA GCCAGGAGAC CGTGAATTCT TCAGCTGCAC GGACAGACGA AACTCCCCAA 
GGAGCTGCAG CCTCTAGTTC TGTTGCAGGG GCTGTGGGCA TGACCACCTC TGGGGAGAGT 
GAATCCGATG ATTCTGAGAT GGGACGACTA CAAGCTTTGT TAGAGGCCAG GGGTCTTCCT 
CCTCATCTGT TTGGCCCTCT CGGTCCTCGG ATGTCACAAC TTTTCCATAG AACAATTGGA 
AGCGGAGCTA GCTCTAAGGC CCAGCAGCTT CTGCAAGGAC TACAAGCAAG TGATGAAAGC 
CAGCAACTCC AGGCAGTTAT TGAAATGTGT CAGTTATTGG TTATGGGAAA TGAGGAGACG 
CTGGGAGGGT TTCCGGTCAA GAGTGTCGTT CCAGCTTTGA TAACATTACT GCAGATGGAG 
CATAATTTCG ATATTATGAA CCATGCTTGT CGAGCCTTAA CATATATGAT GGAAGCACTT 
CCTCGGTCAT CTGCTGTTGT AGTAGATGCA ATTCCTGTCT TTTTAGAAAA GCTGCAAGTT 
ATTCAGTGTA TTGATGTCGC AGAGCAGGCC CTGACTGCCT TGGAGATGTT ATCACGCAGA 
CACAGTAAAG CCATTCTACA GGCGGGTGGT TTGGCAGACT GCTTGCTGTA TCTAGAATTC 
TTCAGCATAA ATGCCCAAAG AAATGCACTA GCAATTGCAG CCAATTGCTG CCAGAGTATC 
ACGCCAGATG AATTTCATTT TGTGGCAGAT TCCCTCCCGT TGCTTACCCA AAGGCTAACC 
CATCAGGACA AAAAGTCAGT TGAAAGCACT TGCCTTTGTT TTGCACGTCT AGTGGACAAC 
TTCCAACATG AGGAGAATTT ACTCCAGCAG GTTGCCTCCA AAGATCTGCT TACGAATGTT 
CAACAACTCT TGGTAGTGAC TCCACCCATT TTAAGTTCTG GGATGTTTAT AATGGTGGTT 
CGCATGTTTT CTCTGATGTG TTCCAACTGT CCAACTTTAG CTGTTCAACT TATGAAGCAA 
AACATTGCAG AAACACTTCA CTTTCTCCTG TGTGGTGCCT CTAATGGAAG TTGTCAGGAA 
CAGATTGATC TTGTTCCACG AAGTCCACAA GAACTGTATG AACTGACATC TCTGATTTGC 
GAACTTATGC CATGTTTACC AAAAGAAGGC ATTTTTGCAG TTGATACCAT GCTGAAGAAG 
GGAAATGCGC AGAACACAGA CGGTGCGATT TGGCAGTGGC GTGATGACCG TGGCCTTTGG 
CATCCCTATA ACAGGATTGA CAGCCGGATC ATTGAGGCAG CCCATCAGGT CGGTGAGGAT 
GAGATAAGCT TGTCCACTCT GGGACGAGTT TATACTATTG ATTTTAATTC TATGCAGCAA 
ATCAATGAGG ACACGGGAAC AGCACGTGCC ATTCAGAGAA AACCTAACCC CTTAGCCAAT 
ACTAACACTA GTGGATATTC AGAGTTAAAG AAGGATGATG CTCGAGCACA GCTTATGAAA 
GAGGATCCGG AACTGGCTAA GTCTTTTATT AAGACATTAT TTGGTGTTCT TTATGAAGTG 
TATAGTTCCT CAGCAGGACC TGCGGTCAGA CATAAGTGCC TTAGAGCAAT TCTTAGGATA 
ATTTATTTTG CGGATGCTGA ACTTCTGAAG GATGTCCTGA AAAATCATGC TGTTTCAAGT 
CACATTGCTT CCATGCTGTC AAGCCAAGAC CTGAAGATAG TAGTTGGAGC ACTTCAGATG 
GCAGAAATCT TAATGCAGAA GTTACCTGAT ATTTTTAGTG TTTACTTCAG AAGAGAAGGT 
GTAATGCATC AAGTAAAACA CTTAGCAGAA TCAGAGTCTT TGTTGACAAG TCCACCAAAG 
GCATGTACGA ATGGGTCAGG ATCCCTGGGA TCCACGACGT CAGTCAGCAG TGGAACAGCC 
ACGGCCGCCA CCAATGCTGC AGCTGACCTG GGGTCCCCCA GCTTGCAGCA CAGCAGAGAT 
GACTCTTTAG ATCTGAGCCC TCAAGGTCGA TTAAGTGATG TTCTAAAGAG AAAACGGCTG 
CCAAAACGAG GGCCAAGAAG GCCAAAGTAT TCACCTCCAA GAGATGATGA CAAAGTAGAC 
AATCAAGCTA AAAGCCCTAC CACTACTCAG TCACCTAAAT CTTCTTTCCT GGCAAGCTTG 
AATCCAAAAA CGTGGGGAAG GTTAAGCGCA CAGTCCAACA GCAACAACAT CGAACCAGCA 
CGAACTGCGG GAGTCAGTGG TCTTGCCAGG GCCGCCTCCA AGGACACCAT ATCCAATAAC 
AGAGAAAAAA TTAAAGGTTG GATTAAGGAA CAGGCGCATA AATTTGTAGA GCGTTATTTC 
AGTTCTGAGA ACATGGATGG AAGCAACCCT GCACTGAATG TCCTTCAGAG ACTTTGTGCT 
GCAACTGAAC AACTCAACCT CCAGGTGGAT GGTGGAGCTG AGTGCCTTGT AGAAATCCGT 
AGCATAGTCT CAGAGTCTGA TGTTTCATCA TTTGAAATCC AACATAGTGG ATTTGTGAAG 
CAGCTGTTAC TTTATTTGAC ATCTAAAAGT GAAAAGGATG CTGTGAGCCG AGAGATCAGA 
TTAAAGAGAT TCCTTCACGT ATTTTTTTCT TCACCACTTC CTGGAGAAGA GCCCATTGGA 
AGAGTAGAAC CAGTGGGTAA TGCGCCTTTG TTGGCATTAG TTCACAAGAT GAACAACTGC 
CTCAGCCAGA TGGAACAGTT TCCAGTCAAA GTACATGACT TCCCCAGTGG GAACGGGACA 
GGAGGCAGAG GATCACAGGC TTTAAAATTT TTCAACACAC ACCAGTTAAA ATGCCAGTTA 
CAAAGACATC CAGACTGTGC AAATGTGAAG CAGTGGAAGG GCGGGCCGGT CAAGATTGAT 
CCCCTGGCTT TGGTACAAGC TATTGAGAGA TACCTTGTAG TTAGAGGGTA TGGAAGAGTA 
AGAGAAGATG ATGAAGACAG TGATGATGAT GGCTCTGATG AGGAAATAGA CGAGTCTCTG 
GCCGCTCAGT TCTTAAATTC CGGAAACGTA AGGCACAGGC TACAGTTTTA TATTGGAGAA 
CATTTGCTAC CATATAACAT GACTGTATAT CAGGCAGTCC GGCAGTTCAG TATACAAGCT 
GAGGATGAGC GGGAGTCCAC AGATGATGAG AGCAACCCTC TGGGAAGAGC TGGTATTTGG 
ACAAAGACTC ATACAATATG GTACAAACCT GTGAGAGAGG ATGAAGAAAG TAATAAAGAT 
TGTGTTGGTG GTAAAAGAGG AAGAGCCCAA ACGGCTCCAA CAAAAACATC CCCTAGAAAT 
GCAAAAAAGC ATGATGAGTT ATGGCATGAT GGAGTATGCC CATCAGTATC CAATCCTTTA 
GAAGTTTACC TCATCTCCTC GCCACCTGAG AATATAACTT TTGAAGACCC GTCCTTAGAT 
GTGATTCTTC TTCTAAGAGT TTTACATGCC ATCAGCCGAT ACTGGTATTA CTTGTATGAT 
AATGCAATGT GCAAGGAGAT TATTCCAACT AGTGAATTTA TTAACAGTAA GTTGACAGCA 
AAAGCAAATA GGCAGCTTCA AGATCCTTTA GTAATCATGA CAGGAAATAT CCCAACGTGG 
CTTACTGAAC TAGGAAAAAC CTGCCCATTT TTCTTCCCTT TTGATACCCG GCAAATGCTT 
TTTTACGTAA CTGCATTTGA TCGGGACCGA GCAATGCAAA GATTACTTGA TACCAACCCA 
GAAATCAACC AGTCGGATTC TCAAGATAGC AGAGTTGCAC CTAGATTGGA TAGAAAAAAA 
CGTACTGTGA ACAGAGAGGA GCTGCTGAAA CAAGCGGAGT CTGTAATGCA GGATCTCGGT 
AGCTCACGGG CCATGTTAGA AATCCAGTAT GAAAATGAGG TTGGTACAGG TCTTGGGCCT 
ACACTGGAGT TTTATGCACT TGTATCTCAG GAACTTCAGA GAGCTGACTT GGGTCTCTGG 
AGAGGTGAAG AAGTGACTCT CAGCAATCCA AAAGGAAGCC AAGAAGGGAC CAAGTATATT 
CAAAACCTCC AGGGCCTGTT TGCACTTCCC TTTGGTAGGA CAGCTAAGCC AGCCCATATC 
GCAAAGGTTA AAATGAAGTT TCGCTTCTTA GGAAAATTAA TGGCCAAGGC TATCATGGAT 
TTCAGATTGG TGGACCTTCC CCTTGGTTTA CCCTTTTATA AGTGGATGCT AAGGCAAGAA 
ACTTCACTGA CGTCGCATGA TTTGTTTGAC ATTGACCCAG TTGTAGCCAG ATCAGTGTAT 
CACCTAGAAG ACATTGTCAG ACAGAAGAAA AGACTTGAGC AAGATAAATC CCAGACCAAA 
GAGAGTCTAC AATATGCATT AGAAACCCTG ACTATGAATG GCTGCTCAGT TGAAGATCTA 
GGATTGGATT TCACGCTGCC AGGGTTTCCC AACATTGAAC TGAAGAAAGG AGGAAAGGAT 
ATACCAGTTA CCATCCACAA TTTAGAGGAG TATCTAAGAC TGGTTATATT CTGGGCACTA 
AATGAAGGCG TTTCCAGGCA ATTTGATTCC TTCAGAGATG GATTTGAATC TGTCTTCCCA 
CTTAGTCATC TTCAGTACTT CTACCCGGAA GAACTGGATC AGCTCTTGTG CGGCAGTAAA 
GCAGACACGT GGGATGCGAA GACACTGATG GAGTGCTGCA GGCCGGACCA CGGTTATACT 
CATGACAGTC GGGCTGTGAA GTTCTTGTTT GAGATTCTCA GTAGTTTTGA TAATGAGCAG 
CAGAGGTTGT TTCTCCAGTT TGTGACAGGT AGCCCAAGAT TGCCTGTTGG AGGCTTCCGG 
AGTCTGAATC CACCTCTGAC AATTGTCCGG AAGACCTTCG AGTCCACGGA GAACCCCGAC 
GACTTCTTGC CCTCTGTAAT GACTTGTGTG AACTACCTTA AGTTGCCGGA CTACTCGAGC 
ATTGACATCA TGCGTGAGAA ACTGTTGATT GCAGCGAGAG AAGGGCAGCA GTCATTCCAT 
CTTTCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_022265374.1
CDS	1..6189
Translation	MSNRPNNNPGGSLRRSQRNTAGAQPQDDSIGGRSHLGQAKHKGYSPPESRKSNSKAPKVQSNTTSELSRGHLSKRSCSSSSAVIVPQPEDPDRANTSERQKTGQVPKKDNSRGVKRSASPDYNRTNSPSSAKKPKALQHTESPSETNKPHSKSKKRHLDQEQQLKSAQSPSTSKAHTRKSGATGGSRSQKRKRTESSCLKSGSVSESTGAEERSAKPTKLASKSAASAKAGCSTITDSSSAASTSSSSSAVASASSTVPPGARVKQGKDQSKARRSRSASSPSPRRSSREKEQSKTGGSSKFDWAARFSPKVSLPKTKLSLPGSSKSETSKPGPSGLQAKLASLRKSTKKRSESPPAELPSLRRSTRQKTTGSCASASRRGSGLGKRGAAEARRQEKMADPESSQETVNSSAARTDETPQGAAASSSVAGAVGMTTSGESESDDSEMGRLQALLEARGLPPHLFGPLGPRMSQLFHRTIGSGASSKAQQLLQGLQASDESQQLQAVIEMCQLLVMGNEETLGGFPVKSVVPALITLLQMEHNFDIMNHACRALTYMMEALPRSSAVVVDAIPVFLEKLQVIQCIDVAEQALTALEMLSRRHSKAILQAGGLADCLLYLEFFSINAQRNALAIAANCCQSITPDEFHFVADSLPLLTQRLTHQDKKSVESTCLCFARLVDNFQHEENLLQQVASKDLLTNVQQLLVVTPPILSSGMFIMVVRMFSLMCSNCPTLAVQLMKQNIAETLHFLLCGASNGSCQEQIDLVPRSPQELYELTSLICELMPCLPKEGIFAVDTMLKKGNAQNTDGAIWQWRDDRGLWHPYNRIDSRIIEAAHQVGEDEISLSTLGRVYTIDFNSMQQINEDTGTARAIQRKPNPLANTNTSGYSELKKDDARAQLMKEDPELAKSFIKTLFGVLYEVYSSSAGPAVRHKCLRAILRIIYFADAELLKDVLKNHAVSSHIASMLSSQDLKIVVGALQMAEILMQKLPDIFSVYFRREGVMHQVKHLAESESLLTSPPKACTNGSGSLGSTTSVSSGTATAATNAAADLGSPSLQHSRDDSLDLSPQGRLSDVLKRKRLPKRGPRRPKYSPPRDDDKVDNQAKSPTTTQSPKSSFLASLNPKTWGRLSAQSNSNNIEPARTAGVSGLARAASKDTISNNREKIKGWIKEQAHKFVERYFSSENMDGSNPALNVLQRLCAATEQLNLQVDGGAECLVEIRSIVSESDVSSFEIQHSGFVKQLLLYLTSKSEKDAVSREIRLKRFLHVFFSSPLPGEEPIGRVEPVGNAPLLALVHKMNNCLSQMEQFPVKVHDFPSGNGTGGRGSQALKFFNTHQLKCQLQRHPDCANVKQWKGGPVKIDPLALVQAIERYLVVRGYGRVREDDEDSDDDGSDEEIDESLAAQFLNSGNVRHRLQFYIGEHLLPYNMTVYQAVRQFSIQAEDERESTDDESNPLGRAGIWTKTHTIWYKPVREDEESNKDCVGGKRGRAQTAPTKTSPRNAKKHDELWHDGVCPSVSNPLEVYLISSPPENITFEDPSLDVILLLRVLHAISRYWYYLYDNAMCKEIIPTSEFINSKLTAKANRQLQDPLVIMTGNIPTWLTELGKTCPFFFPFDTRQMLFYVTAFDRDRAMQRLLDTNPEINQSDSQDSRVAPRLDRKKRTVNREELLKQAESVMQDLGSSRAMLEIQYENEVGTGLGPTLEFYALVSQELQRADLGLWRGEEVTLSNPKGSQEGTKYIQNLQGLFALPFGRTAKPAHIAKVKMKFRFLGKLMAKAIMDFRLVDLPLGLPFYKWMLRQETSLTSHDLFDIDPVVARSVYHLEDIVRQKKRLEQDKSQTKESLQYALETLTMNGCSVEDLGLDFTLPGFPNIELKKGGKDIPVTIHNLEEYLRLVIFWALNEGVSRQFDSFRDGFESVFPLSHLQYFYPEELDQLLCGSKADTWDAKTLMECCRPDHGYTHDSRAVKFLFEILSSFDNEQQRLFLQFVTGSPRLPVGGFRSLNPPLTIVRKTFESTENPDDFLPSVMTCVNYLKLPDYSSIDIMREKLLIAAREGQQSFHLS

Target ORF information:

RefSeq Version	XM_022409666.1
Organism	Canis lupus familiaris(dog)
Definition	Canis lupus familiaris thyroid hormone receptor interactor 12 (TRIP12), transcript variant X6, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_022409666.1

ORF Insert Sequence:

ATGTCCAACC GGCCTAATAA CAATCCAGGG GGGTCACTGC GACGTTCACA GAGGAACACT 
GCCGGGGCCC AACCACAAGA CGACTCGATA GGAGGAAGGT CACATTTAGG GCAGGCAAAA 
CATAAGGGAT ATAGCCCTCC TGAGAGTAGA AAATCTAATT CTAAGGCACC CAAAGTGCAG 
TCTAATACTA CTTCTGAACT GTCAAGGGGA CACCTTTCTA AAAGAAGCTG CAGTTCATCA 
TCTGCTGTCA TAGTTCCACA ACCAGAGGAT CCAGACAGAG CCAATACTTC AGAAAGGCAA 
AAAACGGGGC AGGTGCCTAA GAAAGACAAT TCTCGAGGAG TGAAACGCAG TGCTAGTCCA 
GACTACAACA GGACCAATTC TCCTAGCTCT GCCAAAAAAC CAAAAGCACT TCAGCATACT 
GAATCTCCCT CAGAAACAAA TAAGCCACAT AGTAAGTCGA AGAAGAGACA TTTAGACCAG 
GAGCAACAAC TGAAATCTGC ACAGTCGCCA TCAACAAGTA AGGCTCACAC CAGAAAGAGT 
GGGGCCACCG GCGGCTCACG GAGTCAGAAA AGAAAGAGGA CAGAGAGTTC TTGTCTAAAG 
AGTGGTTCTG TGTCTGAGTC AACTGGTGCC GAAGAGAGAT CTGCAAAACC CACCAAGCTG 
GCTTCAAAAT CCGCCGCCTC GGCCAAAGCT GGGTGCAGCA CCATCACTGA TTCTTCCTCC 
GCTGCCTCTA CTTCCTCCTC ATCTTCTGCT GTCGCCTCGG CCTCCTCCAC TGTACCACCA 
GGGGCCAGGG TGAAACAAGG AAAAGACCAG AGCAAGGCCA GGCGTTCCCG TTCAGCATCC 
AGTCCCAGTC CCAGAAGAAG TAGCAGGGAA AAGGAACAGA GTAAAACTGG TGGCTCTTCA 
AAATTCGATT GGGCTGCTCG TTTCAGCCCT AAAGTTAGCC TTCCTAAAAC CAAACTGTCT 
CTTCCAGGGT CTTCTAAGTC AGAGACATCA AAACCTGGAC CTTCTGGATT ACAGGCCAAA 
TTAGCAAGTT TAAGAAAATC TACCAAGAAG CGCAGTGAAT CCCCACCTGC TGAGCTCCCC 
AGTTTGAGGC GGAGCACACG CCAAAAGACC ACGGGCTCCT GTGCTAGTGC CAGTCGGCGA 
GGCTCTGGCC TGGGCAAAAG AGGAGCAGCT GAAGCTCGTC GACAGGAGAA GATGGCGGAC 
CCTGAAAGCA GCCAGGAGAC CGTGAATTCT TCAGCTGCAC GGACAGACGA AACTCCCCAA 
GGAGCTGCAG CCTCTAGTTC TGTTGCAGGG GCTGTGGGCA TGACCACCTC TGGGGAGAGT 
GAATCCGATG ATTCTGAGAT GGGACGACTA CAAGCTTTGT TAGAGGCCAG GGGTCTTCCT 
CCTCATCTGT TTGGCCCTCT CGGTCCTCGG ATGTCACAAC TTTTCCATAG AACAATTGGA 
AGCGGAGCTA GCTCTAAGGC CCAGCAGCTT CTGCAAGGAC TACAAGCAAG TGATGAAAGC 
CAGCAACTCC AGGCAGTTAT TGAAATGTGT CAGTTATTGG TTATGGGAAA TGAGGAGACG 
CTGGGAGGGT TTCCGGTCAA GAGTGTCGTT CCAGCTTTGA TAACATTACT GCAGATGGAG 
CATAATTTCG ATATTATGAA CCATGCTTGT CGAGCCTTAA CATATATGAT GGAAGCACTT 
CCTCGGTCAT CTGCTGTTGT AGTAGATGCA ATTCCTGTCT TTTTAGAAAA GCTGCAAGTT 
ATTCAGTGTA TTGATGTCGC AGAGCAGGCC CTGACTGCCT TGGAGATGTT ATCACGCAGA 
CACAGTAAAG CCATTCTACA GGCGGGTGGT TTGGCAGACT GCTTGCTGTA TCTAGAATTC 
TTCAGCATAA ATGCCCAAAG AAATGCACTA GCAATTGCAG CCAATTGCTG CCAGAGTATC 
ACGCCAGATG AATTTCATTT TGTGGCAGAT TCCCTCCCGT TGCTTACCCA AAGGCTAACC 
CATCAGGACA AAAAGTCAGT TGAAAGCACT TGCCTTTGTT TTGCACGTCT AGTGGACAAC 
TTCCAACATG AGGAGAATTT ACTCCAGCAG GTTGCCTCCA AAGATCTGCT TACGAATGTT 
CAACAACTCT TGGTAGTGAC TCCACCCATT TTAAGTTCTG GGATGTTTAT AATGGTGGTT 
CGCATGTTTT CTCTGATGTG TTCCAACTGT CCAACTTTAG CTGTTCAACT TATGAAGCAA 
AACATTGCAG AAACACTTCA CTTTCTCCTG TGTGGTGCCT CTAATGGAAG TTGTCAGGAA 
CAGATTGATC TTGTTCCACG AAGTCCACAA GAACTGTATG AACTGACATC TCTGATTTGC 
GAACTTATGC CATGTTTACC AAAAGAAGGC ATTTTTGCAG TTGATACCAT GCTGAAGAAG 
GGAAATGCGC AGAACACAGA CGGTGCGATT TGGCAGTGGC GTGATGACCG TGGCCTTTGG 
CATCCCTATA ACAGGATTGA CAGCCGGATC ATTGAGGCAG CCCATCAGGT CGGTGAGGAT 
GAGATAAGCT TGTCCACTCT GGGACGAGTT TATACTATTG ATTTTAATTC TATGCAGCAA 
ATCAATGAGG ACACGGGAAC AGCACGTGCC ATTCAGAGAA AACCTAACCC CTTAGCCAAT 
ACTAACACTA GTGGATATTC AGAGTTAAAG AAGGATGATG CTCGAGCACA GCTTATGAAA 
GAGGATCCGG AACTGGCTAA GTCTTTTATT AAGACATTAT TTGGTGTTCT TTATGAAGTG 
TATAGTTCCT CAGCAGGACC TGCGGTCAGA CATAAGTGCC TTAGAGCAAT TCTTAGGATA 
ATTTATTTTG CGGATGCTGA ACTTCTGAAG GATGTCCTGA AAAATCATGC TGTTTCAAGT 
CACATTGCTT CCATGCTGTC AAGCCAAGAC CTGAAGATAG TAGTTGGAGC ACTTCAGATG 
GCAGAAATCT TAATGCAGAA GTTACCTGAT ATTTTTAGTG TTTACTTCAG AAGAGAAGGT 
GTAATGCATC AAGTAAAACA CTTAGCAGAA TCAGAGTCTT TGTTGACAAG TCCACCAAAG 
GCATGTACGA ATGGGTCAGG ATCCCTGGGA TCCACGACGT CAGTCAGCAG TGGAACAGCC 
ACGGCCGCCA CCAATGCTGC AGCTGACCTG GGGTCCCCCA GCTTGCAGCA CAGCAGAGAT 
GACTCTTTAG ATCTGAGCCC TCAAGGTCGA TTAAGTGATG TTCTAAAGAG AAAACGGCTG 
CCAAAACGAG GGCCAAGAAG GCCAAAGTAT TCACCTCCAA GAGATGATGA CAAAGTAGAC 
AATCAAGCTA AAAGCCCTAC CACTACTCAG TCACCTAAAT CTTCTTTCCT GGCAAGCTTG 
AATCCAAAAA CGTGGGGAAG GTTAAGCGCA CAGTCCAACA GCAACAACAT CGAACCAGCA 
CGAACTGCGG GAGTCAGTGG TCTTGCCAGG GCCGCCTCCA AGGACACCAT ATCCAATAAC 
AGAGAAAAAA TTAAAGGTTG GATTAAGGAA CAGGCGCATA AATTTGTAGA GCGTTATTTC 
AGTTCTGAGA ACATGGATGG AAGCAACCCT GCACTGAATG TCCTTCAGAG ACTTTGTGCT 
GCAACTGAAC AACTCAACCT CCAGGTGGAT GGTGGAGCTG AGTGCCTTGT AGAAATCCGT 
AGCATAGTCT CAGAGTCTGA TGTTTCATCA TTTGAAATCC AACATAGTGG ATTTGTGAAG 
CAGCTGTTAC TTTATTTGAC ATCTAAAAGT GAAAAGGATG CTGTGAGCCG AGAGATCAGA 
TTAAAGAGAT TCCTTCACGT ATTTTTTTCT TCACCACTTC CTGGAGAAGA GCCCATTGGA 
AGAGTAGAAC CAGTGGGTAA TGCGCCTTTG TTGGCATTAG TTCACAAGAT GAACAACTGC 
CTCAGCCAGA TGGAACAGTT TCCAGTCAAA GTACATGACT TCCCCAGTGG GAACGGGACA 
GGAGGCAGAG GATCACAGGC TTTAAAATTT TTCAACACAC ACCAGTTAAA ATGCCAGTTA 
CAAAGACATC CAGACTGTGC AAATGTGAAG CAGTGGAAGG GCGGGCCGGT CAAGATTGAT 
CCCCTGGCTT TGGTACAAGC TATTGAGAGA TACCTTGTAG TTAGAGGGTA TGGAAGAGTA 
AGAGAAGATG ATGAAGACAG TGATGATGAT GGCTCTGATG AGGAAATAGA CGAGTCTCTG 
GCCGCTCAGT TCTTAAATTC CGGAAACGTA AGGCACAGGC TACAGTTTTA TATTGGAGAA 
CATTTGCTAC CATATAACAT GACTGTATAT CAGGCAGTCC GGCAGTTCAG TATACAAGCT 
GAGGATGAGC GGGAGTCCAC AGATGATGAG AGCAACCCTC TGGGAAGAGC TGGTATTTGG 
ACAAAGACTC ATACAATATG GTACAAACCT GTGAGAGAGG ATGAAGAAAG TAATAAAGAT 
TGTGTTGGTG GTAAAAGAGG AAGAGCCCAA ACGGCTCCAA CAAAAACATC CCCTAGAAAT 
GCAAAAAAGC ATGATGAGTT ATGGCATGAT GGAGTATGCC CATCAGTATC CAATCCTTTA 
GAAGTTTACC TCATCTCCTC GCCACCTGAG AATATAACTT TTGAAGACCC GTCCTTAGAT 
GTGATTCTTC TTCTAAGAGT TTTACATGCC ATCAGCCGAT ACTGGTATTA CTTGTATGAT 
AATGCAATGT GCAAGGAGAT TATTCCAACT AGTGAATTTA TTAACAGTAA GTTGACAGCA 
AAAGCAAATA GGCAGCTTCA AGATCCTTTA GTAATCATGA CAGGAAATAT CCCAACGTGG 
CTTACTGAAC TAGGAAAAAC CTGCCCATTT TTCTTCCCTT TTGATACCCG GCAAATGCTT 
TTTTACGTAA CTGCATTTGA TCGGGACCGA GCAATGCAAA GATTACTTGA TACCAACCCA 
GAAATCAACC AGTCGGATTC TCAAGATAGC AGAGTTGCAC CTAGATTGGA TAGAAAAAAA 
CGTACTGTGA ACAGAGAGGA GCTGCTGAAA CAAGCGGAGT CTGTAATGCA GGATCTCGGT 
AGCTCACGGG CCATGTTAGA AATCCAGTAT GAAAATGAGG TTGGTACAGG TCTTGGGCCT 
ACACTGGAGT TTTATGCACT TGTATCTCAG GAACTTCAGA GAGCTGACTT GGGTCTCTGG 
AGAGGTGAAG AAGTGACTCT CAGCAATCCA AAAGGAAGCC AAGAAGGGAC CAAGTATATT 
CAAAACCTCC AGGGCCTGTT TGCACTTCCC TTTGGTAGGA CAGCTAAGCC AGCCCATATC 
GCAAAGGTTA AAATGAAGTT TCGCTTCTTA GGAAAATTAA TGGCCAAGGC TATCATGGAT 
TTCAGATTGG TGGACCTTCC CCTTGGTTTA CCCTTTTATA AGTGGATGCT AAGGCAAGAA 
ACTTCACTGA CGTCGCATGA TTTGTTTGAC ATTGACCCAG TTGTAGCCAG ATCAGTGTAT 
CACCTAGAAG ACATTGTCAG ACAGAAGAAA AGACTTGAGC AAGATAAATC CCAGACCAAA 
GAGAGTCTAC AATATGCATT AGAAACCCTG ACTATGAATG GCTGCTCAGT TGAAGATCTA 
GGATTGGATT TCACGCTGCC AGGGTTTCCC AACATTGAAC TGAAGAAAGG AGGAAAGGAT 
ATACCAGTTA CCATCCACAA TTTAGAGGAG TATCTAAGAC TGGTTATATT CTGGGCACTA 
AATGAAGGCG TTTCCAGGCA ATTTGATTCC TTCAGAGATG GATTTGAATC TGTCTTCCCA 
CTTAGTCATC TTCAGTACTT CTACCCGGAA GAACTGGATC AGCTCTTGTG CGGCAGTAAA 
GCAGACACGT GGGATGCGAA GACACTGATG GAGTGCTGCA GGCCGGACCA CGGTTATACT 
CATGACAGTC GGGCTGTGAA GTTCTTGTTT GAGATTCTCA GTAGTTTTGA TAATGAGCAG 
CAGAGGTTGT TTCTCCAGTT TGTGACAGGT AGCCCAAGAT TGCCTGTTGG AGGCTTCCGG 
AGTCTGAATC CACCTCTGAC AATTGTCCGG AAGACCTTCG AGTCCACGGA GAACCCCGAC 
GACTTCTTGC CCTCTGTAAT GACTTGTGTG AACTACCTTA AGTTGCCGGA CTACTCGAGC 
ATTGACATCA TGCGTGAGAA ACTGTTGATT GCAGCGAGAG AAGGGCAGCA GTCATTCCAT 
CTTTCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TRIP12 ( XM_022409670.1 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_022265378.1 Canis lupus familiaris thyroid hormone receptor interactor 12 (TRIP12), transcript variant X10, mRNA.

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CloneID	OCe297064
Clone ID Related Accession (Same CDS sequence)	XM_022409670.1
Accession Version	XM_022409670.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6108bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-09-04
Organism	Canis lupus familiaris(dog)
Product	E3 ubiquitin-protein ligase TRIP12 isoform X8
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006607.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCCAACC GGCCTAATAA CAATCCAGGG GGGTCACTGC GACGTTCACA GAGGAACACT 
GCCGGGGCCC AACCACAAGA CGACTCGATA GGAGGAAGGT CACATTTAGG GCAGGCAAAA 
CATAAGGGAT ATAGCCCTCC TGAGAGTAGA AAATCTAATT CTAAGGCACC CAAAGTGCAG 
TCTAATACTA CTTCTGAACT GTCAAGGGGA CACCTTTCTA AAAGAAGCTG CAGTTCATCA 
TCTGCTGTCA TAGTTCCACA ACCAGAGGAT CCAGACAGAG CCAATACTTC AGAAAGGCAA 
AAAACGGGGC AGGTGCCTAA GAAAGACAAT TCTCGAGGAG TGAAACGCAG TGCTAGTCCA 
GACTACAACA GGACCAATTC TCCTAGCTCT GCCAAAAAAC CAAAAGCACT TCAGCATACT 
GAATCTCCCT CAGAAACAAA TAAGCCACAT AGTAAGTCGA AGAAGAGACA TTTAGACCAG 
GAGCAACAAC TGAAATCTGC ACAGTCGCCA TCAACAAGTA AGGCTCACAC CAGAAAGAGT 
GGGGCCACCG GCGGCTCACG GAGTCAGAAA AGAAAGAGGA CAGAGAGTTC TTGTCTAAAG 
AGTGGTTCTG TGTCTGAGTC AACTGGTGCC GAAGAGAGAT CTGCAAAACC CACCAAGCTG 
GCTTCAAAAT CCGCCGCCTC GGCCAAAGCT GGGTGCAGCA CCATCACTGA TTCTTCCTCC 
GCTGCCTCTA CTTCCTCCTC ATCTTCTGCT GTCGCCTCGG CCTCCTCCAC TGTACCACCA 
GGGGCCAGGG TGAAACAAGG AAAAGACCAG AGCAAGGCCA GGCGTTCCCG TTCAGCATCC 
AGTCCCAGTC CCAGAAGAAG TAGCAGGGAA AAGGAACAGA GTAAAACTGG TGGCTCTTCA 
AAATTCGATT GGGCTGCTCG TTTCAGCCCT AAAGTTAGCC TTCCTAAAAC CAAACTGTCT 
CTTCCAGGGT CTTCTAAGTC AGAGACATCA AAACCTGGAC CTTCTGGATT ACAGGCCAAA 
TTAGCAAGTT TAAGAAAATC TACCAAGAAG CGCAGTGAAT CCCCACCTGC TGAGCTCCCC 
AGTTTGAGGC GGAGCACACG CCAAAAGACC ACGGGCTCCT GTGCTAGTGC CAGTCGGCGA 
GGCTCTGGCC TGGGCAAAAG AGGAGCAGCT GAAGCTCGTC GACAGGAGAA GATGGCGGAC 
CCTGAAAGCA GCCAGGAGAC CGTGAATTCT TCAGCTGCAC GGACAGACGA AACTCCCCAA 
GGAGCTGCAG CCTCTAGTTC TGTTGCAGGG GCTGTGGGCA TGACCACCTC TGGGGAGAGT 
GAATCCGATG ATTCTGAGAT GGGACGACTA CAAGCTTTGT TAGAGGCCAG GGGTCTTCCT 
CCTCATCTGT TTGGCCCTCT CGGTCCTCGG ATGTCACAAC TTTTCCATAG AACAATTGGA 
AGCGGAGCTA GCTCTAAGGC CCAGCAGCTT CTGCAAGGAC TACAAGCAAG TGATGAAAGC 
CAGCAACTCC AGGCAGTTAT TGAAATGTGT CAGTTATTGG TTATGGGAAA TGAGGAGACG 
CTGGGAGGGT TTCCGGTCAA GAGTGTCGTT CCAGCTTTGA TAACATTACT GCAGATGGAG 
CATAATTTCG ATATTATGAA CCATGCTTGT CGAGCCTTAA CATATATGAT GGAAGCACTT 
CCTCGGTCAT CTGCTGTTGT AGTAGATGCA ATTCCTGTCT TTTTAGAAAA GCTGCAAGTT 
ATTCAGTGTA TTGATGTCGC AGAGCAGGCC CTGACTGCCT TGGAGATGTT ATCACGCAGA 
CACAGTAAAG CCATTCTACA GGCGGGTGGT TTGGCAGACT GCTTGCTGTA TCTAGAATTC 
TTCAGCATAA ATGCCCAAAG AAATGCACTA GCAATTGCAG CCAATTGCTG CCAGAGTATC 
ACGCCAGATG AATTTCATTT TGTGGCAGAT TCCCTCCCGT TGCTTACCCA AAGGCTAACC 
CATCAGGACA AAAAGTCAGT TGAAAGCACT TGCCTTTGTT TTGCACGTCT AGTGGACAAC 
TTCCAACATG AGGAGAATTT ACTCCAGCAG GTTGCCTCCA AAGATCTGCT TACGAATGTT 
CAACAACTCT TGGTAGTGAC TCCACCCATT TTAAGTTCTG GGATGTTTAT AATGGTGGTT 
CGCATGTTTT CTCTGATGTG TTCCAACTGT CCAACTTTAG CTGTTCAACT TATGAAGCAA 
AACATTGCAG AAACACTTCA CTTTCTCCTG TGTGGTGCCT CTAATGGAAG TTGTCAGGAA 
CAGATTGATC TTGTTCCACG AAGTCCACAA GAACTGTATG AACTGACATC TCTGATTTGC 
GAACTTATGC CATGTTTACC AAAAGAAGGC ATTTTTGCAG TTGATACCAT GCTGAAGAAG 
GGAAATGCGC AGAACACAGA CGGTGCGATT TGGCAGTGGC GTGATGACCG TGGCCTTTGG 
CATCCCTATA ACAGGATTGA CAGCCGGATC ATTGAGGTAG CAGCCCATCA GGTCGGTGAG 
GATGAGATAA GCTTGTCCAC TCTGGGACGA GTTTATACTA TTGATTTTAA TTCTATGCAG 
CAAATCAATG AGGACACGGG AACAGCACGT GCCATTCAGA GAAAACCTAA CCCCTTAGCC 
AATACTAACA CTAGTGGATA TTCAGAGTTA AAGAAGGATG ATGCTCGAGC ACAGCTTATG 
AAAGAGGATC CGGAACTGGC TAAGTCTTTT ATTAAGACAT TATTTGGTGT TCTTTATGAA 
GTGTATAGTT CCTCAGCAGG ACCTGCGGTC AGACATAAGT GCCTTAGAGC AATTCTTAGG 
ATAATTTATT TTGCGGATGC TGAACTTCTG AAGGATGTCC TGAAAAATCA TGCTGTTTCA 
AGTCACATTG CTTCCATGCT GTCAAGCCAA GACCTGAAGA TAGTAGTTGG AGCACTTCAG 
ATGGCAGAAA TCTTAATGCA GAAGTTACCT GATATTTTTA GTGTTTACTT CAGAAGAGAA 
GGTGTAATGC ATCAAGTAAA ACACTTAGCA GAATCAGAGT CTTTGTTGAC AAGTCCACCA 
AAGGCATGTA CGAATGGGTC AGGATCCCTG GGATCCACGA CGTCAGTCAG CAGTGGAACA 
GCCACGGCCG CCACCAATGC TGCAGCTGAC CTGGGGTCCC CCAGCTTGCA GCACAGCAGA 
GATGACTCTT TAGATCTGAG CCCTCAAGGT CGATTAAGTG ATGTTCTAAA GAGAAAACGG 
CTGCCAAAAC GAGGGCCAAG AAGGCCAAAG TATTCACCTC CAAGAGATGA TGACAAAGTA 
GACAATCAAG CTAAAAGCCC TACCACTACT CAGTCACCTA AATCTTCTTT CCTGGCAAGC 
TTGAATCCAA AAACGTGGGG AAGGTTAAGC GCACAGTCCA ACAGCAACAA CATCGAACCA 
GCACGAACTG CGGGAGTCAG TGGTCTTGCC AGGGCCGCCT CCAAGGACAC CATATCCAAT 
AACAGAGAAA AAATTAAAGG TTGGATTAAG GAACAGGCGC ATAAATTTGT AGAGCGTTAT 
TTCAGTTCTG AGAACATGGA TGGAAGCAAC CCTGCACTGA ATGTCCTTCA GAGACTTTGT 
GCTGCAACTG AACAACTCAA CCTCCAGGTG GATGGTGGAG CTGAGTGCCT TGTAGAAATC 
CGTAGCATAG TCTCAGAGTC TGATGTTTCA TCATTTGAAA TCCAACATAG TGGATTTGTG 
AAGCAGCTGT TACTTTATTT GACATCTAAA AGTGAAAAGG ATGCTGTGAG CCGAGAGATC 
AGATTAAAGA GATTCCTTCA CGTATTTTTT TCTTCACCAC TTCCTGGAGA AGAGCCCATT 
GGAAGAGTAG AACCAGTGGG TAATGCGCCT TTGTTGGCAT TAGTTCACAA GATGAACAAC 
TGCCTCAGCC AGATGGAACA GTTTCCAGTC AAAGTACATG ACTTCCCCAG TGGGAACGGG 
ACAGGAGGCA GCTTTTCTCT CAACAGAGGA TCACAGGCTT TAAAATTTTT CAACACACAC 
CAGTTAAAAT GCCAGTTACA AAGACATCCA GACTGTGCAA ATGTGAAGCA GTGGAAGGGC 
GGGCCGGTCA AGATTGATCC CCTGGCTTTG GTACAAGCTA TTGAGAGATA CCTTGTAGTT 
AGAGGGTATG GAAGAGTAAG AGAAGATGAT GAAGACAGTG ATGATGATGG CTCTGATGAG 
GAAATAGACG AGTCTCTGGC CGCTCAGTTC TTAAATTCCG GAAACGTAAG GCACAGGCTA 
CAGTTTTATA TTGGAGAACA TTTGCTACCA TATAACATGA CTGTATATCA GGCAGTCCGG 
CAGTTCAGTA TACAAGCTGA GGATGAGCGG GAGTCCACAG ATGATGAGAG CAACCCTCTG 
GGAAGAGCTG GTATTTGGAC AAAGACTCAT ACAATATGGT ACAAACCTGT GAGAGAGGAT 
GAAGAAAGTA ATAAAGATTG TGTTGGTGGT AAAAGAGGAA GAGCCCAAAC GGCTCCAACA 
AAAACATCCC CTAGAAATGC AAAAAAGCAT GATGAGTTAT GGCATGATGG AGTATGCCCA 
TCAGTATCCA ATCCTTTAGA AGTTTACCTC ATCTCCTCGC CACCTGAGAA TATAACTTTT 
GAAGACCCGT CCTTAGATGT GATTCTTCTT CTAAGAGTTT TACATGCCAT CAGCCGATAC 
TGGTATTACT TGTATGATAA TGCAATGTGC AAGGAGATTA TTCCAACTAG TGAATTTATT 
AACAGTAAGT TGACAGCAAA AGCAAATAGG CAGCTTCAAG ATCCTTTAGT AATCATGACA 
GGAAATATCC CAACGTGGCT TACTGAACTA GGAAAAACCT GCCCATTTTT CTTCCCTTTT 
GATACCCGGC AAATGCTTTT TTACGTAACT GCATTTGATC GGGACCGAGC AATGCAAAGA 
TTACTTGATA CCAACCCAGA AATCAACCAG TCGGATTCTC AAGATAGCAG AGTTGCACCT 
AGATTGGATA GAAAAAAAGT TGGTACAGGT CTTGGGCCTA CACTGGAGTT TTATGCACTT 
GTATCTCAGG AACTTCAGAG AGCTGACTTG GGTCTCTGGA GAGGTGAAGA AGTGACTCTC 
AGCAATCCAA AAGGAAGCCA AGAAGGGACC AAGTATATTC AAAACCTCCA GGGCCTGTTT 
GCACTTCCCT TTGGTAGGAC AGCTAAGCCA GCCCATATCG CAAAGGTTAA AATGAAGTTT 
CGCTTCTTAG GAAAATTAAT GGCCAAGGCT ATCATGGATT TCAGATTGGT GGACCTTCCC 
CTTGGTTTAC CCTTTTATAA GTGGATGCTA AGGCAAGAAA CTTCACTGAC GTCGCATGAT 
TTGTTTGACA TTGACCCAGT TGTAGCCAGA TCAGTGTATC ACCTAGAAGA CATTGTCAGA 
CAGAAGAAAA GACTTGAGCA AGATAAATCC CAGACCAAAG AGAGTCTACA ATATGCATTA 
GAAACCCTGA CTATGAATGG CTGCTCAGTT GAAGATCTAG GATTGGATTT CACGCTGCCA 
GGGTTTCCCA ACATTGAACT GAAGAAAGGA GGAAAGGATA TACCAGTTAC CATCCACAAT 
TTAGAGGAGT ATCTAAGACT GGTTATATTC TGGGCACTAA ATGAAGGCGT TTCCAGGCAA 
TTTGATTCCT TCAGAGATGG ATTTGAATCT GTCTTCCCAC TTAGTCATCT TCAGTACTTC 
TACCCGGAAG AACTGGATCA GCTCTTGTGC GGCAGTAAAG CAGACACGTG GGATGCGAAG 
ACACTGATGG AGTGCTGCAG GCCGGACCAC GGTTATACTC ATGACAGTCG GGCTGTGAAG 
TTCTTGTTTG AGATTCTCAG TAGTTTTGAT AATGAGCAGC AGAGGTTGTT TCTCCAGTTT 
GTGACAGGTA GCCCAAGATT GCCTGTTGGA GGCTTCCGGA GTCTGAATCC ACCTCTGACA 
ATTGTCCGGA AGACCTTCGA GTCCACGGAG AACCCCGACG ACTTCTTGCC CTCTGTAATG 
ACTTGTGTGA ACTACCTTAA GTTGCCGGAC TACTCGAGCA TTGACATCAT GCGTGAGAAA 
CTGTTGATTG CAGCGAGAGA AGGGCAGCAG TCATTCCATC TTTCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_022265378.1
CDS	163..6270
Translation	MSNRPNNNPGGSLRRSQRNTAGAQPQDDSIGGRSHLGQAKHKGYSPPESRKSNSKAPKVQSNTTSELSRGHLSKRSCSSSSAVIVPQPEDPDRANTSERQKTGQVPKKDNSRGVKRSASPDYNRTNSPSSAKKPKALQHTESPSETNKPHSKSKKRHLDQEQQLKSAQSPSTSKAHTRKSGATGGSRSQKRKRTESSCLKSGSVSESTGAEERSAKPTKLASKSAASAKAGCSTITDSSSAASTSSSSSAVASASSTVPPGARVKQGKDQSKARRSRSASSPSPRRSSREKEQSKTGGSSKFDWAARFSPKVSLPKTKLSLPGSSKSETSKPGPSGLQAKLASLRKSTKKRSESPPAELPSLRRSTRQKTTGSCASASRRGSGLGKRGAAEARRQEKMADPESSQETVNSSAARTDETPQGAAASSSVAGAVGMTTSGESESDDSEMGRLQALLEARGLPPHLFGPLGPRMSQLFHRTIGSGASSKAQQLLQGLQASDESQQLQAVIEMCQLLVMGNEETLGGFPVKSVVPALITLLQMEHNFDIMNHACRALTYMMEALPRSSAVVVDAIPVFLEKLQVIQCIDVAEQALTALEMLSRRHSKAILQAGGLADCLLYLEFFSINAQRNALAIAANCCQSITPDEFHFVADSLPLLTQRLTHQDKKSVESTCLCFARLVDNFQHEENLLQQVASKDLLTNVQQLLVVTPPILSSGMFIMVVRMFSLMCSNCPTLAVQLMKQNIAETLHFLLCGASNGSCQEQIDLVPRSPQELYELTSLICELMPCLPKEGIFAVDTMLKKGNAQNTDGAIWQWRDDRGLWHPYNRIDSRIIEVAAHQVGEDEISLSTLGRVYTIDFNSMQQINEDTGTARAIQRKPNPLANTNTSGYSELKKDDARAQLMKEDPELAKSFIKTLFGVLYEVYSSSAGPAVRHKCLRAILRIIYFADAELLKDVLKNHAVSSHIASMLSSQDLKIVVGALQMAEILMQKLPDIFSVYFRREGVMHQVKHLAESESLLTSPPKACTNGSGSLGSTTSVSSGTATAATNAAADLGSPSLQHSRDDSLDLSPQGRLSDVLKRKRLPKRGPRRPKYSPPRDDDKVDNQAKSPTTTQSPKSSFLASLNPKTWGRLSAQSNSNNIEPARTAGVSGLARAASKDTISNNREKIKGWIKEQAHKFVERYFSSENMDGSNPALNVLQRLCAATEQLNLQVDGGAECLVEIRSIVSESDVSSFEIQHSGFVKQLLLYLTSKSEKDAVSREIRLKRFLHVFFSSPLPGEEPIGRVEPVGNAPLLALVHKMNNCLSQMEQFPVKVHDFPSGNGTGGSFSLNRGSQALKFFNTHQLKCQLQRHPDCANVKQWKGGPVKIDPLALVQAIERYLVVRGYGRVREDDEDSDDDGSDEEIDESLAAQFLNSGNVRHRLQFYIGEHLLPYNMTVYQAVRQFSIQAEDERESTDDESNPLGRAGIWTKTHTIWYKPVREDEESNKDCVGGKRGRAQTAPTKTSPRNAKKHDELWHDGVCPSVSNPLEVYLISSPPENITFEDPSLDVILLLRVLHAISRYWYYLYDNAMCKEIIPTSEFINSKLTAKANRQLQDPLVIMTGNIPTWLTELGKTCPFFFPFDTRQMLFYVTAFDRDRAMQRLLDTNPEINQSDSQDSRVAPRLDRKKVGTGLGPTLEFYALVSQELQRADLGLWRGEEVTLSNPKGSQEGTKYIQNLQGLFALPFGRTAKPAHIAKVKMKFRFLGKLMAKAIMDFRLVDLPLGLPFYKWMLRQETSLTSHDLFDIDPVVARSVYHLEDIVRQKKRLEQDKSQTKESLQYALETLTMNGCSVEDLGLDFTLPGFPNIELKKGGKDIPVTIHNLEEYLRLVIFWALNEGVSRQFDSFRDGFESVFPLSHLQYFYPEELDQLLCGSKADTWDAKTLMECCRPDHGYTHDSRAVKFLFEILSSFDNEQQRLFLQFVTGSPRLPVGGFRSLNPPLTIVRKTFESTENPDDFLPSVMTCVNYLKLPDYSSIDIMREKLLIAAREGQQSFHLS

Target ORF information:

RefSeq Version	XM_022409670.1
Organism	Canis lupus familiaris(dog)
Definition	Canis lupus familiaris thyroid hormone receptor interactor 12 (TRIP12), transcript variant X10, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_022409670.1

ORF Insert Sequence:

ATGTCCAACC GGCCTAATAA CAATCCAGGG GGGTCACTGC GACGTTCACA GAGGAACACT 
GCCGGGGCCC AACCACAAGA CGACTCGATA GGAGGAAGGT CACATTTAGG GCAGGCAAAA 
CATAAGGGAT ATAGCCCTCC TGAGAGTAGA AAATCTAATT CTAAGGCACC CAAAGTGCAG 
TCTAATACTA CTTCTGAACT GTCAAGGGGA CACCTTTCTA AAAGAAGCTG CAGTTCATCA 
TCTGCTGTCA TAGTTCCACA ACCAGAGGAT CCAGACAGAG CCAATACTTC AGAAAGGCAA 
AAAACGGGGC AGGTGCCTAA GAAAGACAAT TCTCGAGGAG TGAAACGCAG TGCTAGTCCA 
GACTACAACA GGACCAATTC TCCTAGCTCT GCCAAAAAAC CAAAAGCACT TCAGCATACT 
GAATCTCCCT CAGAAACAAA TAAGCCACAT AGTAAGTCGA AGAAGAGACA TTTAGACCAG 
GAGCAACAAC TGAAATCTGC ACAGTCGCCA TCAACAAGTA AGGCTCACAC CAGAAAGAGT 
GGGGCCACCG GCGGCTCACG GAGTCAGAAA AGAAAGAGGA CAGAGAGTTC TTGTCTAAAG 
AGTGGTTCTG TGTCTGAGTC AACTGGTGCC GAAGAGAGAT CTGCAAAACC CACCAAGCTG 
GCTTCAAAAT CCGCCGCCTC GGCCAAAGCT GGGTGCAGCA CCATCACTGA TTCTTCCTCC 
GCTGCCTCTA CTTCCTCCTC ATCTTCTGCT GTCGCCTCGG CCTCCTCCAC TGTACCACCA 
GGGGCCAGGG TGAAACAAGG AAAAGACCAG AGCAAGGCCA GGCGTTCCCG TTCAGCATCC 
AGTCCCAGTC CCAGAAGAAG TAGCAGGGAA AAGGAACAGA GTAAAACTGG TGGCTCTTCA 
AAATTCGATT GGGCTGCTCG TTTCAGCCCT AAAGTTAGCC TTCCTAAAAC CAAACTGTCT 
CTTCCAGGGT CTTCTAAGTC AGAGACATCA AAACCTGGAC CTTCTGGATT ACAGGCCAAA 
TTAGCAAGTT TAAGAAAATC TACCAAGAAG CGCAGTGAAT CCCCACCTGC TGAGCTCCCC 
AGTTTGAGGC GGAGCACACG CCAAAAGACC ACGGGCTCCT GTGCTAGTGC CAGTCGGCGA 
GGCTCTGGCC TGGGCAAAAG AGGAGCAGCT GAAGCTCGTC GACAGGAGAA GATGGCGGAC 
CCTGAAAGCA GCCAGGAGAC CGTGAATTCT TCAGCTGCAC GGACAGACGA AACTCCCCAA 
GGAGCTGCAG CCTCTAGTTC TGTTGCAGGG GCTGTGGGCA TGACCACCTC TGGGGAGAGT 
GAATCCGATG ATTCTGAGAT GGGACGACTA CAAGCTTTGT TAGAGGCCAG GGGTCTTCCT 
CCTCATCTGT TTGGCCCTCT CGGTCCTCGG ATGTCACAAC TTTTCCATAG AACAATTGGA 
AGCGGAGCTA GCTCTAAGGC CCAGCAGCTT CTGCAAGGAC TACAAGCAAG TGATGAAAGC 
CAGCAACTCC AGGCAGTTAT TGAAATGTGT CAGTTATTGG TTATGGGAAA TGAGGAGACG 
CTGGGAGGGT TTCCGGTCAA GAGTGTCGTT CCAGCTTTGA TAACATTACT GCAGATGGAG 
CATAATTTCG ATATTATGAA CCATGCTTGT CGAGCCTTAA CATATATGAT GGAAGCACTT 
CCTCGGTCAT CTGCTGTTGT AGTAGATGCA ATTCCTGTCT TTTTAGAAAA GCTGCAAGTT 
ATTCAGTGTA TTGATGTCGC AGAGCAGGCC CTGACTGCCT TGGAGATGTT ATCACGCAGA 
CACAGTAAAG CCATTCTACA GGCGGGTGGT TTGGCAGACT GCTTGCTGTA TCTAGAATTC 
TTCAGCATAA ATGCCCAAAG AAATGCACTA GCAATTGCAG CCAATTGCTG CCAGAGTATC 
ACGCCAGATG AATTTCATTT TGTGGCAGAT TCCCTCCCGT TGCTTACCCA AAGGCTAACC 
CATCAGGACA AAAAGTCAGT TGAAAGCACT TGCCTTTGTT TTGCACGTCT AGTGGACAAC 
TTCCAACATG AGGAGAATTT ACTCCAGCAG GTTGCCTCCA AAGATCTGCT TACGAATGTT 
CAACAACTCT TGGTAGTGAC TCCACCCATT TTAAGTTCTG GGATGTTTAT AATGGTGGTT 
CGCATGTTTT CTCTGATGTG TTCCAACTGT CCAACTTTAG CTGTTCAACT TATGAAGCAA 
AACATTGCAG AAACACTTCA CTTTCTCCTG TGTGGTGCCT CTAATGGAAG TTGTCAGGAA 
CAGATTGATC TTGTTCCACG AAGTCCACAA GAACTGTATG AACTGACATC TCTGATTTGC 
GAACTTATGC CATGTTTACC AAAAGAAGGC ATTTTTGCAG TTGATACCAT GCTGAAGAAG 
GGAAATGCGC AGAACACAGA CGGTGCGATT TGGCAGTGGC GTGATGACCG TGGCCTTTGG 
CATCCCTATA ACAGGATTGA CAGCCGGATC ATTGAGGTAG CAGCCCATCA GGTCGGTGAG 
GATGAGATAA GCTTGTCCAC TCTGGGACGA GTTTATACTA TTGATTTTAA TTCTATGCAG 
CAAATCAATG AGGACACGGG AACAGCACGT GCCATTCAGA GAAAACCTAA CCCCTTAGCC 
AATACTAACA CTAGTGGATA TTCAGAGTTA AAGAAGGATG ATGCTCGAGC ACAGCTTATG 
AAAGAGGATC CGGAACTGGC TAAGTCTTTT ATTAAGACAT TATTTGGTGT TCTTTATGAA 
GTGTATAGTT CCTCAGCAGG ACCTGCGGTC AGACATAAGT GCCTTAGAGC AATTCTTAGG 
ATAATTTATT TTGCGGATGC TGAACTTCTG AAGGATGTCC TGAAAAATCA TGCTGTTTCA 
AGTCACATTG CTTCCATGCT GTCAAGCCAA GACCTGAAGA TAGTAGTTGG AGCACTTCAG 
ATGGCAGAAA TCTTAATGCA GAAGTTACCT GATATTTTTA GTGTTTACTT CAGAAGAGAA 
GGTGTAATGC ATCAAGTAAA ACACTTAGCA GAATCAGAGT CTTTGTTGAC AAGTCCACCA 
AAGGCATGTA CGAATGGGTC AGGATCCCTG GGATCCACGA CGTCAGTCAG CAGTGGAACA 
GCCACGGCCG CCACCAATGC TGCAGCTGAC CTGGGGTCCC CCAGCTTGCA GCACAGCAGA 
GATGACTCTT TAGATCTGAG CCCTCAAGGT CGATTAAGTG ATGTTCTAAA GAGAAAACGG 
CTGCCAAAAC GAGGGCCAAG AAGGCCAAAG TATTCACCTC CAAGAGATGA TGACAAAGTA 
GACAATCAAG CTAAAAGCCC TACCACTACT CAGTCACCTA AATCTTCTTT CCTGGCAAGC 
TTGAATCCAA AAACGTGGGG AAGGTTAAGC GCACAGTCCA ACAGCAACAA CATCGAACCA 
GCACGAACTG CGGGAGTCAG TGGTCTTGCC AGGGCCGCCT CCAAGGACAC CATATCCAAT 
AACAGAGAAA AAATTAAAGG TTGGATTAAG GAACAGGCGC ATAAATTTGT AGAGCGTTAT 
TTCAGTTCTG AGAACATGGA TGGAAGCAAC CCTGCACTGA ATGTCCTTCA GAGACTTTGT 
GCTGCAACTG AACAACTCAA CCTCCAGGTG GATGGTGGAG CTGAGTGCCT TGTAGAAATC 
CGTAGCATAG TCTCAGAGTC TGATGTTTCA TCATTTGAAA TCCAACATAG TGGATTTGTG 
AAGCAGCTGT TACTTTATTT GACATCTAAA AGTGAAAAGG ATGCTGTGAG CCGAGAGATC 
AGATTAAAGA GATTCCTTCA CGTATTTTTT TCTTCACCAC TTCCTGGAGA AGAGCCCATT 
GGAAGAGTAG AACCAGTGGG TAATGCGCCT TTGTTGGCAT TAGTTCACAA GATGAACAAC 
TGCCTCAGCC AGATGGAACA GTTTCCAGTC AAAGTACATG ACTTCCCCAG TGGGAACGGG 
ACAGGAGGCA GCTTTTCTCT CAACAGAGGA TCACAGGCTT TAAAATTTTT CAACACACAC 
CAGTTAAAAT GCCAGTTACA AAGACATCCA GACTGTGCAA ATGTGAAGCA GTGGAAGGGC 
GGGCCGGTCA AGATTGATCC CCTGGCTTTG GTACAAGCTA TTGAGAGATA CCTTGTAGTT 
AGAGGGTATG GAAGAGTAAG AGAAGATGAT GAAGACAGTG ATGATGATGG CTCTGATGAG 
GAAATAGACG AGTCTCTGGC CGCTCAGTTC TTAAATTCCG GAAACGTAAG GCACAGGCTA 
CAGTTTTATA TTGGAGAACA TTTGCTACCA TATAACATGA CTGTATATCA GGCAGTCCGG 
CAGTTCAGTA TACAAGCTGA GGATGAGCGG GAGTCCACAG ATGATGAGAG CAACCCTCTG 
GGAAGAGCTG GTATTTGGAC AAAGACTCAT ACAATATGGT ACAAACCTGT GAGAGAGGAT 
GAAGAAAGTA ATAAAGATTG TGTTGGTGGT AAAAGAGGAA GAGCCCAAAC GGCTCCAACA 
AAAACATCCC CTAGAAATGC AAAAAAGCAT GATGAGTTAT GGCATGATGG AGTATGCCCA 
TCAGTATCCA ATCCTTTAGA AGTTTACCTC ATCTCCTCGC CACCTGAGAA TATAACTTTT 
GAAGACCCGT CCTTAGATGT GATTCTTCTT CTAAGAGTTT TACATGCCAT CAGCCGATAC 
TGGTATTACT TGTATGATAA TGCAATGTGC AAGGAGATTA TTCCAACTAG TGAATTTATT 
AACAGTAAGT TGACAGCAAA AGCAAATAGG CAGCTTCAAG ATCCTTTAGT AATCATGACA 
GGAAATATCC CAACGTGGCT TACTGAACTA GGAAAAACCT GCCCATTTTT CTTCCCTTTT 
GATACCCGGC AAATGCTTTT TTACGTAACT GCATTTGATC GGGACCGAGC AATGCAAAGA 
TTACTTGATA CCAACCCAGA AATCAACCAG TCGGATTCTC AAGATAGCAG AGTTGCACCT 
AGATTGGATA GAAAAAAAGT TGGTACAGGT CTTGGGCCTA CACTGGAGTT TTATGCACTT 
GTATCTCAGG AACTTCAGAG AGCTGACTTG GGTCTCTGGA GAGGTGAAGA AGTGACTCTC 
AGCAATCCAA AAGGAAGCCA AGAAGGGACC AAGTATATTC AAAACCTCCA GGGCCTGTTT 
GCACTTCCCT TTGGTAGGAC AGCTAAGCCA GCCCATATCG CAAAGGTTAA AATGAAGTTT 
CGCTTCTTAG GAAAATTAAT GGCCAAGGCT ATCATGGATT TCAGATTGGT GGACCTTCCC 
CTTGGTTTAC CCTTTTATAA GTGGATGCTA AGGCAAGAAA CTTCACTGAC GTCGCATGAT 
TTGTTTGACA TTGACCCAGT TGTAGCCAGA TCAGTGTATC ACCTAGAAGA CATTGTCAGA 
CAGAAGAAAA GACTTGAGCA AGATAAATCC CAGACCAAAG AGAGTCTACA ATATGCATTA 
GAAACCCTGA CTATGAATGG CTGCTCAGTT GAAGATCTAG GATTGGATTT CACGCTGCCA 
GGGTTTCCCA ACATTGAACT GAAGAAAGGA GGAAAGGATA TACCAGTTAC CATCCACAAT 
TTAGAGGAGT ATCTAAGACT GGTTATATTC TGGGCACTAA ATGAAGGCGT TTCCAGGCAA 
TTTGATTCCT TCAGAGATGG ATTTGAATCT GTCTTCCCAC TTAGTCATCT TCAGTACTTC 
TACCCGGAAG AACTGGATCA GCTCTTGTGC GGCAGTAAAG CAGACACGTG GGATGCGAAG 
ACACTGATGG AGTGCTGCAG GCCGGACCAC GGTTATACTC ATGACAGTCG GGCTGTGAAG 
TTCTTGTTTG AGATTCTCAG TAGTTTTGAT AATGAGCAGC AGAGGTTGTT TCTCCAGTTT 
GTGACAGGTA GCCCAAGATT GCCTGTTGGA GGCTTCCGGA GTCTGAATCC ACCTCTGACA 
ATTGTCCGGA AGACCTTCGA GTCCACGGAG AACCCCGACG ACTTCTTGCC CTCTGTAATG 
ACTTGTGTGA ACTACCTTAA GTTGCCGGAC TACTCGAGCA TTGACATCAT GCGTGAGAAA 
CTGTTGATTG CAGCGAGAGA AGGGCAGCAG TCATTCCATC TTTCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TRIP12 ( XM_022409671.1 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_022265379.1 Canis lupus familiaris thyroid hormone receptor interactor 12 (TRIP12), transcript variant X11, mRNA.

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CloneID	OCe297065
Clone ID Related Accession (Same CDS sequence)	XM_022409671.1
Accession Version	XM_022409671.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6102bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-09-04
Organism	Canis lupus familiaris(dog)
Product	E3 ubiquitin-protein ligase TRIP12 isoform X9
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006607.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCCAACC GGCCTAATAA CAATCCAGGG GGGTCACTGC GACGTTCACA GAGGAACACT 
GCCGGGGCCC AACCACAAGA CGACTCGATA GGAGGAAGGT CACATTTAGG GCAGGCAAAA 
CATAAGGGAT ATAGCCCTCC TGAGAGTAGA AAATCTAATT CTAAGGCACC CAAAGTGCAG 
TCTAATACTA CTTCTGAACT GTCAAGGGGA CACCTTTCTA AAAGAAGCTG CAGTTCATCA 
TCTGCTGTCA TAGTTCCACA ACCAGAGGAT CCAGACAGAG CCAATACTTC AGAAAGGCAA 
AAAACGGGGC AGGTGCCTAA GAAAGACAAT TCTCGAGGAG TGAAACGCAG TGCTAGTCCA 
GACTACAACA GGACCAATTC TCCTAGCTCT GCCAAAAAAC CAAAAGCACT TCAGCATACT 
GAATCTCCCT CAGAAACAAA TAAGCCACAT AGTAAGTCGA AGAAGAGACA TTTAGACCAG 
GAGCAACAAC TGAAATCTGC ACAGTCGCCA TCAACAAGTA AGGCTCACAC CAGAAAGAGT 
GGGGCCACCG GCGGCTCACG GAGTCAGAAA AGAAAGAGGA CAGAGAGTTC TTGTCTAAAG 
AGTGGTTCTG TGTCTGAGTC AACTGGTGCC GAAGAGAGAT CTGCAAAACC CACCAAGCTG 
GCTTCAAAAT CCGCCGCCTC GGCCAAAGCT GGGTGCAGCA CCATCACTGA TTCTTCCTCC 
GCTGCCTCTA CTTCCTCCTC ATCTTCTGCT GTCGCCTCGG CCTCCTCCAC TGTACCACCA 
GGGGCCAGGG TGAAACAAGG AAAAGACCAG AGCAAGGCCA GGCGTTCCCG TTCAGCATCC 
AGTCCCAGTC CCAGAAGAAG TAGCAGGGAA AAGGAACAGA GGTCTTCTAA GTCAGAGACA 
TCAAAACCTG GACCTTCTGG ATTACAGGCC AAATTAGCAA GTTTAAGAAA ATCTACCAAG 
AAGCGCAGTG AATCCCCACC TGCTGAGCTC CCCAGTTTGA GGCGGAGCAC ACGCCAAAAG 
ACCACGGGCT CCTGTGCTAG TGCCAGTCGG CGAGGCTCTG GCCTGGGCAA AAGAGGAGCA 
GCTGAAGCTC GTCGACAGGA GAAGATGGCG GACCCTGAAA GCAGCCAGGA GACCGTGAAT 
TCTTCAGCTG CACGGACAGA CGAAACTCCC CAAGGAGCTG CAGCCTCTAG TTCTGTTGCA 
GGGGCTGTGG GCATGACCAC CTCTGGGGAG AGTGAATCCG ATGATTCTGA GATGGGACGA 
CTACAAGCTT TGTTAGAGGC CAGGGGTCTT CCTCCTCATC TGTTTGGCCC TCTCGGTCCT 
CGGATGTCAC AACTTTTCCA TAGAACAATT GGAAGCGGAG CTAGCTCTAA GGCCCAGCAG 
CTTCTGCAAG GACTACAAGC AAGTGATGAA AGCCAGCAAC TCCAGGCAGT TATTGAAATG 
TGTCAGTTAT TGGTTATGGG AAATGAGGAG ACGCTGGGAG GGTTTCCGGT CAAGAGTGTC 
GTTCCAGCTT TGATAACATT ACTGCAGATG GAGCATAATT TCGATATTAT GAACCATGCT 
TGTCGAGCCT TAACATATAT GATGGAAGCA CTTCCTCGGT CATCTGCTGT TGTAGTAGAT 
GCAATTCCTG TCTTTTTAGA AAAGCTGCAA GTTATTCAGT GTATTGATGT CGCAGAGCAG 
GCCCTGACTG CCTTGGAGAT GTTATCACGC AGACACAGTA AAGCCATTCT ACAGGCGGGT 
GGTTTGGCAG ACTGCTTGCT GTATCTAGAA TTCTTCAGCA TAAATGCCCA AAGAAATGCA 
CTAGCAATTG CAGCCAATTG CTGCCAGAGT ATCACGCCAG ATGAATTTCA TTTTGTGGCA 
GATTCCCTCC CGTTGCTTAC CCAAAGGCTA ACCCATCAGG ACAAAAAGTC AGTTGAAAGC 
ACTTGCCTTT GTTTTGCACG TCTAGTGGAC AACTTCCAAC ATGAGGAGAA TTTACTCCAG 
CAGGTTGCCT CCAAAGATCT GCTTACGAAT GTTCAACAAC TCTTGGTAGT GACTCCACCC 
ATTTTAAGTT CTGGGATGTT TATAATGGTG GTTCGCATGT TTTCTCTGAT GTGTTCCAAC 
TGTCCAACTT TAGCTGTTCA ACTTATGAAG CAAAACATTG CAGAAACACT TCACTTTCTC 
CTGTGTGGTG CCTCTAATGG AAGTTGTCAG GAACAGATTG ATCTTGTTCC ACGAAGTCCA 
CAAGAACTGT ATGAACTGAC ATCTCTGATT TGCGAACTTA TGCCATGTTT ACCAAAAGAA 
GGCATTTTTG CAGTTGATAC CATGCTGAAG AAGGGAAATG CGCAGAACAC AGACGGTGCG 
ATTTGGCAGT GGCGTGATGA CCGTGGCCTT TGGCATCCCT ATAACAGGAT TGACAGCCGG 
ATCATTGAGG CAGCCCATCA GGTCGGTGAG GATGAGATAA GCTTGTCCAC TCTGGGACGA 
GTTTATACTA TTGATTTTAA TTCTATGCAG CAAATCAATG AGGACACGGG AACAGCACGT 
GCCATTCAGA GAAAACCTAA CCCCTTAGCC AATACTAACA CTAGTGGATA TTCAGAGTTA 
AAGAAGGATG ATGCTCGAGC ACAGCTTATG AAAGAGGATC CGGAACTGGC TAAGTCTTTT 
ATTAAGACAT TATTTGGTGT TCTTTATGAA GTGTATAGTT CCTCAGCAGG ACCTGCGGTC 
AGACATAAGT GCCTTAGAGC AATTCTTAGG ATAATTTATT TTGCGGATGC TGAACTTCTG 
AAGGATGTCC TGAAAAATCA TGCTGTTTCA AGTCACATTG CTTCCATGCT GTCAAGCCAA 
GACCTGAAGA TAGTAGTTGG AGCACTTCAG ATGGCAGAAA TCTTAATGCA GAAGTTACCT 
GATATTTTTA GTGTTTACTT CAGAAGAGAA GGTGTAATGC ATCAAGTAAA ACACTTAGCA 
GAATCAGAGT CTTTGTTGAC AAGTCCACCA AAGGCATGTA CGAATGGGTC AGGATCCCTG 
GGATCCACGA CGTCAGTCAG CAGTGGAACA GCCACGGCCG CCACCAATGC TGCAGCTGAC 
CTGGGGTCCC CCAGCTTGCA GCACAGCAGA GATGACTCTT TAGATCTGAG CCCTCAAGGT 
CGATTAAGTG ATGTTCTAAA GAGAAAACGG CTGCCAAAAC GAGGGCCAAG AAGGCCAAAG 
TATTCACCTC CAAGAGATGA TGACAAAGTA GACAATCAAG CTAAAAGCCC TACCACTACT 
CAGTCACCTA AATCTTCTTT CCTGGCAAGC TTGAATCCAA AAACGTGGGG AAGGTTAAGC 
GCACAGTCCA ACAGCAACAA CATCGAACCA GCACGAACTG CGGGAGTCAG TGGTCTTGCC 
AGGGCCGCCT CCAAGGACAC CATATCCAAT AACAGAGAAA AAATTAAAGG TTGGATTAAG 
GAACAGGCGC ATAAATTTGT AGAGCGTTAT TTCAGTTCTG AGAACATGGA TGGAAGCAAC 
CCTGCACTGA ATGTCCTTCA GAGACTTTGT GCTGCAACTG AACAACTCAA CCTCCAGGTG 
GATGGTGGAG CTGAGTGCCT TGTAGAAATC CGTAGCATAG TCTCAGAGTC TGATGTTTCA 
TCATTTGAAA TCCAACATAG TGGATTTGTG AAGCAGCTGT TACTTTATTT GACATCTAAA 
AGTGAAAAGG ATGCTGTGAG CCGAGAGATC AGATTAAAGA GATTCCTTCA CGTATTTTTT 
TCTTCACCAC TTCCTGGAGA AGAGCCCATT GGAAGAGTAG AACCAGTGGG TAATGCGCCT 
TTGTTGGCAT TAGTTCACAA GATGAACAAC TGCCTCAGCC AGATGGAACA GTTTCCAGTC 
AAAGTACATG ACTTCCCCAG TGGGAACGGG ACAGGAGGCA GAGGATCACA GGCTTTAAAA 
TTTTTCAACA CACACCAGTT AAAATGCCAG TTACAAAGAC ATCCAGACTG TGCAAATGTG 
AAGCAGTGGA AGGGCGGGCC GGTCAAGATT GATCCCCTGG CTTTGGTACA AGCTATTGAG 
AGATACCTTG TAGTTAGAGG GTATGGAAGA GTAAGAGAAG ATGATGAAGA CAGTGATGAT 
GATGGCTCTG ATGAGGAAAT AGACGAGTCT CTGGCCGCTC AGTTCTTAAA TTCCGGAAAC 
GTAAGGCACA GGCTACAGTT TTATATTGGA GAACATTTGC TACCATATAA CATGACTGTA 
TATCAGGCAG TCCGGCAGTT CAGTATACAA GCTGAGGATG AGCGGGAGTC CACAGATGAT 
GAGAGCAACC CTCTGGGAAG AGCTGGTATT TGGACAAAGA CTCATACAAT ATGGTACAAA 
CCTGTGAGAG AGGATGAAGA AAGTAATAAA GATTGTGTTG GTGGTAAAAG AGGAAGAGCC 
CAAACGGCTC CAACAAAAAC ATCCCCTAGA AATGCAAAAA AGCATGATGA GTTATGGCAT 
GATGGAGTAT GCCCATCAGT ATCCAATCCT TTAGAAGTTT ACCTCATCTC CTCGCCACCT 
GAGAATATAA CTTTTGAAGA CCCGTCCTTA GATGTGATTC TTCTTCTAAG AGTTTTACAT 
GCCATCAGCC GATACTGGTA TTACTTGTAT GATAATGCAA TGTGCAAGGA GATTATTCCA 
ACTAGTGAAT TTATTAACAG TAAGTTGACA GCAAAAGCAA ATAGGCAGCT TCAAGATCCT 
TTAGTAATCA TGACAGGAAA TATCCCAACG TGGCTTACTG AACTAGGAAA AACCTGCCCA 
TTTTTCTTCC CTTTTGATAC CCGGCAAATG CTTTTTTACG TAACTGCATT TGATCGGGAC 
CGAGCAATGC AAAGATTACT TGATACCAAC CCAGAAATCA ACCAGTCGGA TTCTCAAGAT 
AGCAGAGTTG CACCTAGATT GGATAGAAAA AAACGTACTG TGAACAGAGA GGAGCTGCTG 
AAACAAGCGG AGTCTGTAAT GCAGGATCTC GGTAGCTCAC GGGCCATGTT AGAAATCCAG 
TATGAAAATG AGGTTGGTAC AGGTCTTGGG CCTACACTGG AGTTTTATGC ACTTGTATCT 
CAGGAACTTC AGAGAGCTGA CTTGGGTCTC TGGAGAGGTG AAGAAGTGAC TCTCAGCAAT 
CCAAAAGGAA GCCAAGAAGG GACCAAGTAT ATTCAAAACC TCCAGGGCCT GTTTGCACTT 
CCCTTTGGTA GGACAGCTAA GCCAGCCCAT ATCGCAAAGG TTAAAATGAA GTTTCGCTTC 
TTAGGAAAAT TAATGGCCAA GGCTATCATG GATTTCAGAT TGGTGGACCT TCCCCTTGGT 
TTACCCTTTT ATAAGTGGAT GCTAAGGCAA GAAACTTCAC TGACGTCGCA TGATTTGTTT 
GACATTGACC CAGTTGTAGC CAGATCAGTG TATCACCTAG AAGACATTGT CAGACAGAAG 
AAAAGACTTG AGCAAGATAA ATCCCAGACC AAAGAGAGTC TACAATATGC ATTAGAAACC 
CTGACTATGA ATGGCTGCTC AGTTGAAGAT CTAGGATTGG ATTTCACGCT GCCAGGGTTT 
CCCAACATTG AACTGAAGAA AGGAGGAAAG GATATACCAG TTACCATCCA CAATTTAGAG 
GAGTATCTAA GACTGGTTAT ATTCTGGGCA CTAAATGAAG GCGTTTCCAG GCAATTTGAT 
TCCTTCAGAG ATGGATTTGA ATCTGTCTTC CCACTTAGTC ATCTTCAGTA CTTCTACCCG 
GAAGAACTGG ATCAGCTCTT GTGCGGCAGT AAAGCAGACA CGTGGGATGC GAAGACACTG 
ATGGAGTGCT GCAGGCCGGA CCACGGTTAT ACTCATGACA GTCGGGCTGT GAAGTTCTTG 
TTTGAGATTC TCAGTAGTTT TGATAATGAG CAGCAGAGGT TGTTTCTCCA GTTTGTGACA 
GGTAGCCCAA GATTGCCTGT TGGAGGCTTC CGGAGTCTGA ATCCACCTCT GACAATTGTC 
CGGAAGACCT TCGAGTCCAC GGAGAACCCC GACGACTTCT TGCCCTCTGT AATGACTTGT 
GTGAACTACC TTAAGTTGCC GGACTACTCG AGCATTGACA TCATGCGTGA GAAACTGTTG 
ATTGCAGCGA GAGAAGGGCA GCAGTCATTC CATCTTTCCT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_022265379.1
CDS	1..6102
Translation	MSNRPNNNPGGSLRRSQRNTAGAQPQDDSIGGRSHLGQAKHKGYSPPESRKSNSKAPKVQSNTTSELSRGHLSKRSCSSSSAVIVPQPEDPDRANTSERQKTGQVPKKDNSRGVKRSASPDYNRTNSPSSAKKPKALQHTESPSETNKPHSKSKKRHLDQEQQLKSAQSPSTSKAHTRKSGATGGSRSQKRKRTESSCLKSGSVSESTGAEERSAKPTKLASKSAASAKAGCSTITDSSSAASTSSSSSAVASASSTVPPGARVKQGKDQSKARRSRSASSPSPRRSSREKEQRSSKSETSKPGPSGLQAKLASLRKSTKKRSESPPAELPSLRRSTRQKTTGSCASASRRGSGLGKRGAAEARRQEKMADPESSQETVNSSAARTDETPQGAAASSSVAGAVGMTTSGESESDDSEMGRLQALLEARGLPPHLFGPLGPRMSQLFHRTIGSGASSKAQQLLQGLQASDESQQLQAVIEMCQLLVMGNEETLGGFPVKSVVPALITLLQMEHNFDIMNHACRALTYMMEALPRSSAVVVDAIPVFLEKLQVIQCIDVAEQALTALEMLSRRHSKAILQAGGLADCLLYLEFFSINAQRNALAIAANCCQSITPDEFHFVADSLPLLTQRLTHQDKKSVESTCLCFARLVDNFQHEENLLQQVASKDLLTNVQQLLVVTPPILSSGMFIMVVRMFSLMCSNCPTLAVQLMKQNIAETLHFLLCGASNGSCQEQIDLVPRSPQELYELTSLICELMPCLPKEGIFAVDTMLKKGNAQNTDGAIWQWRDDRGLWHPYNRIDSRIIEAAHQVGEDEISLSTLGRVYTIDFNSMQQINEDTGTARAIQRKPNPLANTNTSGYSELKKDDARAQLMKEDPELAKSFIKTLFGVLYEVYSSSAGPAVRHKCLRAILRIIYFADAELLKDVLKNHAVSSHIASMLSSQDLKIVVGALQMAEILMQKLPDIFSVYFRREGVMHQVKHLAESESLLTSPPKACTNGSGSLGSTTSVSSGTATAATNAAADLGSPSLQHSRDDSLDLSPQGRLSDVLKRKRLPKRGPRRPKYSPPRDDDKVDNQAKSPTTTQSPKSSFLASLNPKTWGRLSAQSNSNNIEPARTAGVSGLARAASKDTISNNREKIKGWIKEQAHKFVERYFSSENMDGSNPALNVLQRLCAATEQLNLQVDGGAECLVEIRSIVSESDVSSFEIQHSGFVKQLLLYLTSKSEKDAVSREIRLKRFLHVFFSSPLPGEEPIGRVEPVGNAPLLALVHKMNNCLSQMEQFPVKVHDFPSGNGTGGRGSQALKFFNTHQLKCQLQRHPDCANVKQWKGGPVKIDPLALVQAIERYLVVRGYGRVREDDEDSDDDGSDEEIDESLAAQFLNSGNVRHRLQFYIGEHLLPYNMTVYQAVRQFSIQAEDERESTDDESNPLGRAGIWTKTHTIWYKPVREDEESNKDCVGGKRGRAQTAPTKTSPRNAKKHDELWHDGVCPSVSNPLEVYLISSPPENITFEDPSLDVILLLRVLHAISRYWYYLYDNAMCKEIIPTSEFINSKLTAKANRQLQDPLVIMTGNIPTWLTELGKTCPFFFPFDTRQMLFYVTAFDRDRAMQRLLDTNPEINQSDSQDSRVAPRLDRKKRTVNREELLKQAESVMQDLGSSRAMLEIQYENEVGTGLGPTLEFYALVSQELQRADLGLWRGEEVTLSNPKGSQEGTKYIQNLQGLFALPFGRTAKPAHIAKVKMKFRFLGKLMAKAIMDFRLVDLPLGLPFYKWMLRQETSLTSHDLFDIDPVVARSVYHLEDIVRQKKRLEQDKSQTKESLQYALETLTMNGCSVEDLGLDFTLPGFPNIELKKGGKDIPVTIHNLEEYLRLVIFWALNEGVSRQFDSFRDGFESVFPLSHLQYFYPEELDQLLCGSKADTWDAKTLMECCRPDHGYTHDSRAVKFLFEILSSFDNEQQRLFLQFVTGSPRLPVGGFRSLNPPLTIVRKTFESTENPDDFLPSVMTCVNYLKLPDYSSIDIMREKLLIAAREGQQSFHLS

Target ORF information:

RefSeq Version	XM_022409671.1
Organism	Canis lupus familiaris(dog)
Definition	Canis lupus familiaris thyroid hormone receptor interactor 12 (TRIP12), transcript variant X11, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_022409671.1

ORF Insert Sequence:

ATGTCCAACC GGCCTAATAA CAATCCAGGG GGGTCACTGC GACGTTCACA GAGGAACACT 
GCCGGGGCCC AACCACAAGA CGACTCGATA GGAGGAAGGT CACATTTAGG GCAGGCAAAA 
CATAAGGGAT ATAGCCCTCC TGAGAGTAGA AAATCTAATT CTAAGGCACC CAAAGTGCAG 
TCTAATACTA CTTCTGAACT GTCAAGGGGA CACCTTTCTA AAAGAAGCTG CAGTTCATCA 
TCTGCTGTCA TAGTTCCACA ACCAGAGGAT CCAGACAGAG CCAATACTTC AGAAAGGCAA 
AAAACGGGGC AGGTGCCTAA GAAAGACAAT TCTCGAGGAG TGAAACGCAG TGCTAGTCCA 
GACTACAACA GGACCAATTC TCCTAGCTCT GCCAAAAAAC CAAAAGCACT TCAGCATACT 
GAATCTCCCT CAGAAACAAA TAAGCCACAT AGTAAGTCGA AGAAGAGACA TTTAGACCAG 
GAGCAACAAC TGAAATCTGC ACAGTCGCCA TCAACAAGTA AGGCTCACAC CAGAAAGAGT 
GGGGCCACCG GCGGCTCACG GAGTCAGAAA AGAAAGAGGA CAGAGAGTTC TTGTCTAAAG 
AGTGGTTCTG TGTCTGAGTC AACTGGTGCC GAAGAGAGAT CTGCAAAACC CACCAAGCTG 
GCTTCAAAAT CCGCCGCCTC GGCCAAAGCT GGGTGCAGCA CCATCACTGA TTCTTCCTCC 
GCTGCCTCTA CTTCCTCCTC ATCTTCTGCT GTCGCCTCGG CCTCCTCCAC TGTACCACCA 
GGGGCCAGGG TGAAACAAGG AAAAGACCAG AGCAAGGCCA GGCGTTCCCG TTCAGCATCC 
AGTCCCAGTC CCAGAAGAAG TAGCAGGGAA AAGGAACAGA GGTCTTCTAA GTCAGAGACA 
TCAAAACCTG GACCTTCTGG ATTACAGGCC AAATTAGCAA GTTTAAGAAA ATCTACCAAG 
AAGCGCAGTG AATCCCCACC TGCTGAGCTC CCCAGTTTGA GGCGGAGCAC ACGCCAAAAG 
ACCACGGGCT CCTGTGCTAG TGCCAGTCGG CGAGGCTCTG GCCTGGGCAA AAGAGGAGCA 
GCTGAAGCTC GTCGACAGGA GAAGATGGCG GACCCTGAAA GCAGCCAGGA GACCGTGAAT 
TCTTCAGCTG CACGGACAGA CGAAACTCCC CAAGGAGCTG CAGCCTCTAG TTCTGTTGCA 
GGGGCTGTGG GCATGACCAC CTCTGGGGAG AGTGAATCCG ATGATTCTGA GATGGGACGA 
CTACAAGCTT TGTTAGAGGC CAGGGGTCTT CCTCCTCATC TGTTTGGCCC TCTCGGTCCT 
CGGATGTCAC AACTTTTCCA TAGAACAATT GGAAGCGGAG CTAGCTCTAA GGCCCAGCAG 
CTTCTGCAAG GACTACAAGC AAGTGATGAA AGCCAGCAAC TCCAGGCAGT TATTGAAATG 
TGTCAGTTAT TGGTTATGGG AAATGAGGAG ACGCTGGGAG GGTTTCCGGT CAAGAGTGTC 
GTTCCAGCTT TGATAACATT ACTGCAGATG GAGCATAATT TCGATATTAT GAACCATGCT 
TGTCGAGCCT TAACATATAT GATGGAAGCA CTTCCTCGGT CATCTGCTGT TGTAGTAGAT 
GCAATTCCTG TCTTTTTAGA AAAGCTGCAA GTTATTCAGT GTATTGATGT CGCAGAGCAG 
GCCCTGACTG CCTTGGAGAT GTTATCACGC AGACACAGTA AAGCCATTCT ACAGGCGGGT 
GGTTTGGCAG ACTGCTTGCT GTATCTAGAA TTCTTCAGCA TAAATGCCCA AAGAAATGCA 
CTAGCAATTG CAGCCAATTG CTGCCAGAGT ATCACGCCAG ATGAATTTCA TTTTGTGGCA 
GATTCCCTCC CGTTGCTTAC CCAAAGGCTA ACCCATCAGG ACAAAAAGTC AGTTGAAAGC 
ACTTGCCTTT GTTTTGCACG TCTAGTGGAC AACTTCCAAC ATGAGGAGAA TTTACTCCAG 
CAGGTTGCCT CCAAAGATCT GCTTACGAAT GTTCAACAAC TCTTGGTAGT GACTCCACCC 
ATTTTAAGTT CTGGGATGTT TATAATGGTG GTTCGCATGT TTTCTCTGAT GTGTTCCAAC 
TGTCCAACTT TAGCTGTTCA ACTTATGAAG CAAAACATTG CAGAAACACT TCACTTTCTC 
CTGTGTGGTG CCTCTAATGG AAGTTGTCAG GAACAGATTG ATCTTGTTCC ACGAAGTCCA 
CAAGAACTGT ATGAACTGAC ATCTCTGATT TGCGAACTTA TGCCATGTTT ACCAAAAGAA 
GGCATTTTTG CAGTTGATAC CATGCTGAAG AAGGGAAATG CGCAGAACAC AGACGGTGCG 
ATTTGGCAGT GGCGTGATGA CCGTGGCCTT TGGCATCCCT ATAACAGGAT TGACAGCCGG 
ATCATTGAGG CAGCCCATCA GGTCGGTGAG GATGAGATAA GCTTGTCCAC TCTGGGACGA 
GTTTATACTA TTGATTTTAA TTCTATGCAG CAAATCAATG AGGACACGGG AACAGCACGT 
GCCATTCAGA GAAAACCTAA CCCCTTAGCC AATACTAACA CTAGTGGATA TTCAGAGTTA 
AAGAAGGATG ATGCTCGAGC ACAGCTTATG AAAGAGGATC CGGAACTGGC TAAGTCTTTT 
ATTAAGACAT TATTTGGTGT TCTTTATGAA GTGTATAGTT CCTCAGCAGG ACCTGCGGTC 
AGACATAAGT GCCTTAGAGC AATTCTTAGG ATAATTTATT TTGCGGATGC TGAACTTCTG 
AAGGATGTCC TGAAAAATCA TGCTGTTTCA AGTCACATTG CTTCCATGCT GTCAAGCCAA 
GACCTGAAGA TAGTAGTTGG AGCACTTCAG ATGGCAGAAA TCTTAATGCA GAAGTTACCT 
GATATTTTTA GTGTTTACTT CAGAAGAGAA GGTGTAATGC ATCAAGTAAA ACACTTAGCA 
GAATCAGAGT CTTTGTTGAC AAGTCCACCA AAGGCATGTA CGAATGGGTC AGGATCCCTG 
GGATCCACGA CGTCAGTCAG CAGTGGAACA GCCACGGCCG CCACCAATGC TGCAGCTGAC 
CTGGGGTCCC CCAGCTTGCA GCACAGCAGA GATGACTCTT TAGATCTGAG CCCTCAAGGT 
CGATTAAGTG ATGTTCTAAA GAGAAAACGG CTGCCAAAAC GAGGGCCAAG AAGGCCAAAG 
TATTCACCTC CAAGAGATGA TGACAAAGTA GACAATCAAG CTAAAAGCCC TACCACTACT 
CAGTCACCTA AATCTTCTTT CCTGGCAAGC TTGAATCCAA AAACGTGGGG AAGGTTAAGC 
GCACAGTCCA ACAGCAACAA CATCGAACCA GCACGAACTG CGGGAGTCAG TGGTCTTGCC 
AGGGCCGCCT CCAAGGACAC CATATCCAAT AACAGAGAAA AAATTAAAGG TTGGATTAAG 
GAACAGGCGC ATAAATTTGT AGAGCGTTAT TTCAGTTCTG AGAACATGGA TGGAAGCAAC 
CCTGCACTGA ATGTCCTTCA GAGACTTTGT GCTGCAACTG AACAACTCAA CCTCCAGGTG 
GATGGTGGAG CTGAGTGCCT TGTAGAAATC CGTAGCATAG TCTCAGAGTC TGATGTTTCA 
TCATTTGAAA TCCAACATAG TGGATTTGTG AAGCAGCTGT TACTTTATTT GACATCTAAA 
AGTGAAAAGG ATGCTGTGAG CCGAGAGATC AGATTAAAGA GATTCCTTCA CGTATTTTTT 
TCTTCACCAC TTCCTGGAGA AGAGCCCATT GGAAGAGTAG AACCAGTGGG TAATGCGCCT 
TTGTTGGCAT TAGTTCACAA GATGAACAAC TGCCTCAGCC AGATGGAACA GTTTCCAGTC 
AAAGTACATG ACTTCCCCAG TGGGAACGGG ACAGGAGGCA GAGGATCACA GGCTTTAAAA 
TTTTTCAACA CACACCAGTT AAAATGCCAG TTACAAAGAC ATCCAGACTG TGCAAATGTG 
AAGCAGTGGA AGGGCGGGCC GGTCAAGATT GATCCCCTGG CTTTGGTACA AGCTATTGAG 
AGATACCTTG TAGTTAGAGG GTATGGAAGA GTAAGAGAAG ATGATGAAGA CAGTGATGAT 
GATGGCTCTG ATGAGGAAAT AGACGAGTCT CTGGCCGCTC AGTTCTTAAA TTCCGGAAAC 
GTAAGGCACA GGCTACAGTT TTATATTGGA GAACATTTGC TACCATATAA CATGACTGTA 
TATCAGGCAG TCCGGCAGTT CAGTATACAA GCTGAGGATG AGCGGGAGTC CACAGATGAT 
GAGAGCAACC CTCTGGGAAG AGCTGGTATT TGGACAAAGA CTCATACAAT ATGGTACAAA 
CCTGTGAGAG AGGATGAAGA AAGTAATAAA GATTGTGTTG GTGGTAAAAG AGGAAGAGCC 
CAAACGGCTC CAACAAAAAC ATCCCCTAGA AATGCAAAAA AGCATGATGA GTTATGGCAT 
GATGGAGTAT GCCCATCAGT ATCCAATCCT TTAGAAGTTT ACCTCATCTC CTCGCCACCT 
GAGAATATAA CTTTTGAAGA CCCGTCCTTA GATGTGATTC TTCTTCTAAG AGTTTTACAT 
GCCATCAGCC GATACTGGTA TTACTTGTAT GATAATGCAA TGTGCAAGGA GATTATTCCA 
ACTAGTGAAT TTATTAACAG TAAGTTGACA GCAAAAGCAA ATAGGCAGCT TCAAGATCCT 
TTAGTAATCA TGACAGGAAA TATCCCAACG TGGCTTACTG AACTAGGAAA AACCTGCCCA 
TTTTTCTTCC CTTTTGATAC CCGGCAAATG CTTTTTTACG TAACTGCATT TGATCGGGAC 
CGAGCAATGC AAAGATTACT TGATACCAAC CCAGAAATCA ACCAGTCGGA TTCTCAAGAT 
AGCAGAGTTG CACCTAGATT GGATAGAAAA AAACGTACTG TGAACAGAGA GGAGCTGCTG 
AAACAAGCGG AGTCTGTAAT GCAGGATCTC GGTAGCTCAC GGGCCATGTT AGAAATCCAG 
TATGAAAATG AGGTTGGTAC AGGTCTTGGG CCTACACTGG AGTTTTATGC ACTTGTATCT 
CAGGAACTTC AGAGAGCTGA CTTGGGTCTC TGGAGAGGTG AAGAAGTGAC TCTCAGCAAT 
CCAAAAGGAA GCCAAGAAGG GACCAAGTAT ATTCAAAACC TCCAGGGCCT GTTTGCACTT 
CCCTTTGGTA GGACAGCTAA GCCAGCCCAT ATCGCAAAGG TTAAAATGAA GTTTCGCTTC 
TTAGGAAAAT TAATGGCCAA GGCTATCATG GATTTCAGAT TGGTGGACCT TCCCCTTGGT 
TTACCCTTTT ATAAGTGGAT GCTAAGGCAA GAAACTTCAC TGACGTCGCA TGATTTGTTT 
GACATTGACC CAGTTGTAGC CAGATCAGTG TATCACCTAG AAGACATTGT CAGACAGAAG 
AAAAGACTTG AGCAAGATAA ATCCCAGACC AAAGAGAGTC TACAATATGC ATTAGAAACC 
CTGACTATGA ATGGCTGCTC AGTTGAAGAT CTAGGATTGG ATTTCACGCT GCCAGGGTTT 
CCCAACATTG AACTGAAGAA AGGAGGAAAG GATATACCAG TTACCATCCA CAATTTAGAG 
GAGTATCTAA GACTGGTTAT ATTCTGGGCA CTAAATGAAG GCGTTTCCAG GCAATTTGAT 
TCCTTCAGAG ATGGATTTGA ATCTGTCTTC CCACTTAGTC ATCTTCAGTA CTTCTACCCG 
GAAGAACTGG ATCAGCTCTT GTGCGGCAGT AAAGCAGACA CGTGGGATGC GAAGACACTG 
ATGGAGTGCT GCAGGCCGGA CCACGGTTAT ACTCATGACA GTCGGGCTGT GAAGTTCTTG 
TTTGAGATTC TCAGTAGTTT TGATAATGAG CAGCAGAGGT TGTTTCTCCA GTTTGTGACA 
GGTAGCCCAA GATTGCCTGT TGGAGGCTTC CGGAGTCTGA ATCCACCTCT GACAATTGTC 
CGGAAGACCT TCGAGTCCAC GGAGAACCCC GACGACTTCT TGCCCTCTGT AATGACTTGT 
GTGAACTACC TTAAGTTGCC GGACTACTCG AGCATTGACA TCATGCGTGA GAAACTGTTG 
ATTGCAGCGA GAGAAGGGCA GCAGTCATTC CATCTTTCCT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TRIP12 ( XM_022409674.1 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_022265382.1 Canis lupus familiaris thyroid hormone receptor interactor 12 (TRIP12), transcript variant X14, mRNA.

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CloneID	OCe297066
Clone ID Related Accession (Same CDS sequence)	XM_022409674.1
Accession Version	XM_022409674.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6063bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-09-04
Organism	Canis lupus familiaris(dog)
Product	E3 ubiquitin-protein ligase TRIP12 isoform X12
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006607.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCCAACC GGCCTAATAA CAATCCAGGG GGGTCACTGC GACGTTCACA GAGGAACACT 
GCCGGGGCCC AACCACAAGA CGACTCGATA GGAGGAAGAA GCTGCAGTTC ATCATCTGCT 
GTCATAGTTC CACAACCAGA GGATCCAGAC AGAGCCAATA CTTCAGAAAG GCAAAAAACG 
GGGCAGGTGC CTAAGAAAGA CAATTCTCGA GGAGTGAAAC GCAGTGCTAG TCCAGACTAC 
AACAGGACCA ATTCTCCTAG CTCTGCCAAA AAACCAAAAG CACTTCAGCA TACTGAATCT 
CCCTCAGAAA CAAATAAGCC ACATAGTAAG TCGAAGAAGA GACATTTAGA CCAGGAGCAA 
CAACTGAAAT CTGCACAGTC GCCATCAACA AGTAAGGCTC ACACCAGAAA GAGTGGGGCC 
ACCGGCGGCT CACGGAGTCA GAAAAGAAAG AGGACAGAGA GTTCTTGTCT AAAGAGTGGT 
TCTGTGTCTG AGTCAACTGG TGCCGAAGAG AGATCTGCAA AACCCACCAA GCTGGCTTCA 
AAATCCGCCG CCTCGGCCAA AGCTGGGTGC AGCACCATCA CTGATTCTTC CTCCGCTGCC 
TCTACTTCCT CCTCATCTTC TGCTGTCGCC TCGGCCTCCT CCACTGTACC ACCAGGGGCC 
AGGGTGAAAC AAGGAAAAGA CCAGAGCAAG GCCAGGCGTT CCCGTTCAGC ATCCAGTCCC 
AGTCCCAGAA GAAGTAGCAG GGAAAAGGAA CAGAGTAAAA CTGGTGGCTC TTCAAAATTC 
GATTGGGCTG CTCGTTTCAG CCCTAAAGTT AGCCTTCCTA AAACCAAACT GTCTCTTCCA 
GGGTCTTCTA AGTCAGAGAC ATCAAAACCT GGACCTTCTG GATTACAGGC CAAATTAGCA 
AGTTTAAGAA AATCTACCAA GAAGCGCAGT GAATCCCCAC CTGCTGAGCT CCCCAGTTTG 
AGGCGGAGCA CACGCCAAAA GACCACGGGC TCCTGTGCTA GTGCCAGTCG GCGAGGCTCT 
GGCCTGGGCA AAAGAGGAGC AGCTGAAGCT CGTCGACAGG AGAAGATGGC GGACCCTGAA 
AGCAGCCAGG AGACCGTGAA TTCTTCAGCT GCACGGACAG ACGAAACTCC CCAAGGAGCT 
GCAGCCTCTA GTTCTGTTGC AGGGGCTGTG GGCATGACCA CCTCTGGGGA GAGTGAATCC 
GATGATTCTG AGATGGGACG ACTACAAGCT TTGTTAGAGG CCAGGGGTCT TCCTCCTCAT 
CTGTTTGGCC CTCTCGGTCC TCGGATGTCA CAACTTTTCC ATAGAACAAT TGGAAGCGGA 
GCTAGCTCTA AGGCCCAGCA GCTTCTGCAA GGACTACAAG CAAGTGATGA AAGCCAGCAA 
CTCCAGGCAG TTATTGAAAT GTGTCAGTTA TTGGTTATGG GAAATGAGGA GACGCTGGGA 
GGGTTTCCGG TCAAGAGTGT CGTTCCAGCT TTGATAACAT TACTGCAGAT GGAGCATAAT 
TTCGATATTA TGAACCATGC TTGTCGAGCC TTAACATATA TGATGGAAGC ACTTCCTCGG 
TCATCTGCTG TTGTAGTAGA TGCAATTCCT GTCTTTTTAG AAAAGCTGCA AGTTATTCAG 
TGTATTGATG TCGCAGAGCA GGCCCTGACT GCCTTGGAGA TGTTATCACG CAGACACAGT 
AAAGCCATTC TACAGGCGGG TGGTTTGGCA GACTGCTTGC TGTATCTAGA ATTCTTCAGC 
ATAAATGCCC AAAGAAATGC ACTAGCAATT GCAGCCAATT GCTGCCAGAG TATCACGCCA 
GATGAATTTC ATTTTGTGGC AGATTCCCTC CCGTTGCTTA CCCAAAGGCT AACCCATCAG 
GACAAAAAGT CAGTTGAAAG CACTTGCCTT TGTTTTGCAC GTCTAGTGGA CAACTTCCAA 
CATGAGGAGA ATTTACTCCA GCAGGTTGCC TCCAAAGATC TGCTTACGAA TGTTCAACAA 
CTCTTGGTAG TGACTCCACC CATTTTAAGT TCTGGGATGT TTATAATGGT GGTTCGCATG 
TTTTCTCTGA TGTGTTCCAA CTGTCCAACT TTAGCTGTTC AACTTATGAA GCAAAACATT 
GCAGAAACAC TTCACTTTCT CCTGTGTGGT GCCTCTAATG GAAGTTGTCA GGAACAGATT 
GATCTTGTTC CACGAAGTCC ACAAGAACTG TATGAACTGA CATCTCTGAT TTGCGAACTT 
ATGCCATGTT TACCAAAAGA AGGCATTTTT GCAGTTGATA CCATGCTGAA GAAGGGAAAT 
GCGCAGAACA CAGACGGTGC GATTTGGCAG TGGCGTGATG ACCGTGGCCT TTGGCATCCC 
TATAACAGGA TTGACAGCCG GATCATTGAG GCAGCCCATC AGGTCGGTGA GGATGAGATA 
AGCTTGTCCA CTCTGGGACG AGTTTATACT ATTGATTTTA ATTCTATGCA GCAAATCAAT 
GAGGACACGG GAACAGCACG TGCCATTCAG AGAAAACCTA ACCCCTTAGC CAATACTAAC 
ACTAGTGGAT ATTCAGAGTT AAAGAAGGAT GATGCTCGAG CACAGCTTAT GAAAGAGGAT 
CCGGAACTGG CTAAGTCTTT TATTAAGACA TTATTTGGTG TTCTTTATGA AGTGTATAGT 
TCCTCAGCAG GACCTGCGGT CAGACATAAG TGCCTTAGAG CAATTCTTAG GATAATTTAT 
TTTGCGGATG CTGAACTTCT GAAGGATGTC CTGAAAAATC ATGCTGTTTC AAGTCACATT 
GCTTCCATGC TGTCAAGCCA AGACCTGAAG ATAGTAGTTG GAGCACTTCA GATGGCAGAA 
ATCTTAATGC AGAAGTTACC TGATATTTTT AGTGTTTACT TCAGAAGAGA AGGTGTAATG 
CATCAAGTAA AACACTTAGC AGAATCAGAG TCTTTGTTGA CAAGTCCACC AAAGGCATGT 
ACGAATGGGT CAGGATCCCT GGGATCCACG ACGTCAGTCA GCAGTGGAAC AGCCACGGCC 
GCCACCAATG CTGCAGCTGA CCTGGGGTCC CCCAGCTTGC AGCACAGCAG AGATGACTCT 
TTAGATCTGA GCCCTCAAGG TCGATTAAGT GATGTTCTAA AGAGAAAACG GCTGCCAAAA 
CGAGGGCCAA GAAGGCCAAA GTATTCACCT CCAAGAGATG ATGACAAAGT AGACAATCAA 
GCTAAAAGCC CTACCACTAC TCAGTCACCT AAATCTTCTT TCCTGGCAAG CTTGAATCCA 
AAAACGTGGG GAAGGTTAAG CGCACAGTCC AACAGCAACA ACATCGAACC AGCACGAACT 
GCGGGAGTCA GTGGTCTTGC CAGGGCCGCC TCCAAGGACA CCATATCCAA TAACAGAGAA 
AAAATTAAAG GTTGGATTAA GGAACAGGCG CATAAATTTG TAGAGCGTTA TTTCAGTTCT 
GAGAACATGG ATGGAAGCAA CCCTGCACTG AATGTCCTTC AGAGACTTTG TGCTGCAACT 
GAACAACTCA ACCTCCAGGT GGATGGTGGA GCTGAGTGCC TTGTAGAAAT CCGTAGCATA 
GTCTCAGAGT CTGATGTTTC ATCATTTGAA ATCCAACATA GTGGATTTGT GAAGCAGCTG 
TTACTTTATT TGACATCTAA AAGTGAAAAG GATGCTGTGA GCCGAGAGAT CAGATTAAAG 
AGATTCCTTC ACGTATTTTT TTCTTCACCA CTTCCTGGAG AAGAGCCCAT TGGAAGAGTA 
GAACCAGTGG GTAATGCGCC TTTGTTGGCA TTAGTTCACA AGATGAACAA CTGCCTCAGC 
CAGATGGAAC AGTTTCCAGT CAAAGTACAT GACTTCCCCA GTGGGAACGG GACAGGAGGC 
AGAGGATCAC AGGCTTTAAA ATTTTTCAAC ACACACCAGT TAAAATGCCA GTTACAAAGA 
CATCCAGACT GTGCAAATGT GAAGCAGTGG AAGGGCGGGC CGGTCAAGAT TGATCCCCTG 
GCTTTGGTAC AAGCTATTGA GAGATACCTT GTAGTTAGAG GGTATGGAAG AGTAAGAGAA 
GATGATGAAG ACAGTGATGA TGATGGCTCT GATGAGGAAA TAGACGAGTC TCTGGCCGCT 
CAGTTCTTAA ATTCCGGAAA CGTAAGGCAC AGGCTACAGT TTTATATTGG AGAACATTTG 
CTACCATATA ACATGACTGT ATATCAGGCA GTCCGGCAGT TCAGTATACA AGCTGAGGAT 
GAGCGGGAGT CCACAGATGA TGAGAGCAAC CCTCTGGGAA GAGCTGGTAT TTGGACAAAG 
ACTCATACAA TATGGTACAA ACCTGTGAGA GAGGATGAAG AAAGTAATAA AGATTGTGTT 
GGTGGTAAAA GAGGAAGAGC CCAAACGGCT CCAACAAAAA CATCCCCTAG AAATGCAAAA 
AAGCATGATG AGTTATGGCA TGATGGAGTA TGCCCATCAG TATCCAATCC TTTAGAAGTT 
TACCTCATCT CCTCGCCACC TGAGAATATA ACTTTTGAAG ACCCGTCCTT AGATGTGATT 
CTTCTTCTAA GAGTTTTACA TGCCATCAGC CGATACTGGT ATTACTTGTA TGATAATGCA 
ATGTGCAAGG AGATTATTCC AACTAGTGAA TTTATTAACA GTAAGTTGAC AGCAAAAGCA 
AATAGGCAGC TTCAAGATCC TTTAGTAATC ATGACAGGAA ATATCCCAAC GTGGCTTACT 
GAACTAGGAA AAACCTGCCC ATTTTTCTTC CCTTTTGATA CCCGGCAAAT GCTTTTTTAC 
GTAACTGCAT TTGATCGGGA CCGAGCAATG CAAAGATTAC TTGATACCAA CCCAGAAATC 
AACCAGTCGG ATTCTCAAGA TAGCAGAGTT GCACCTAGAT TGGATAGAAA AAAACGTACT 
GTGAACAGAG AGGAGCTGCT GAAACAAGCG GAGTCTGTAA TGCAGGATCT CGGTAGCTCA 
CGGGCCATGT TAGAAATCCA GTATGAAAAT GAGGTTGGTA CAGGTCTTGG GCCTACACTG 
GAGTTTTATG CACTTGTATC TCAGGAACTT CAGAGAGCTG ACTTGGGTCT CTGGAGAGGT 
GAAGAAGTGA CTCTCAGCAA TCCAAAAGGA AGCCAAGAAG GGACCAAGTA TATTCAAAAC 
CTCCAGGGCC TGTTTGCACT TCCCTTTGGT AGGACAGCTA AGCCAGCCCA TATCGCAAAG 
GTTAAAATGA AGTTTCGCTT CTTAGGAAAA TTAATGGCCA AGGCTATCAT GGATTTCAGA 
TTGGTGGACC TTCCCCTTGG TTTACCCTTT TATAAGTGGA TGCTAAGGCA AGAAACTTCA 
CTGACGTCGC ATGATTTGTT TGACATTGAC CCAGTTGTAG CCAGATCAGT GTATCACCTA 
GAAGACATTG TCAGACAGAA GAAAAGACTT GAGCAAGATA AATCCCAGAC CAAAGAGAGT 
CTACAATATG CATTAGAAAC CCTGACTATG AATGGCTGCT CAGTTGAAGA TCTAGGATTG 
GATTTCACGC TGCCAGGGTT TCCCAACATT GAACTGAAGA AAGGAGGAAA GGATATACCA 
GTTACCATCC ACAATTTAGA GGAGTATCTA AGACTGGTTA TATTCTGGGC ACTAAATGAA 
GGCGTTTCCA GGCAATTTGA TTCCTTCAGA GATGGATTTG AATCTGTCTT CCCACTTAGT 
CATCTTCAGT ACTTCTACCC GGAAGAACTG GATCAGCTCT TGTGCGGCAG TAAAGCAGAC 
ACGTGGGATG CGAAGACACT GATGGAGTGC TGCAGGCCGG ACCACGGTTA TACTCATGAC 
AGTCGGGCTG TGAAGTTCTT GTTTGAGATT CTCAGTAGTT TTGATAATGA GCAGCAGAGG 
TTGTTTCTCC AGTTTGTGAC AGGTAGCCCA AGATTGCCTG TTGGAGGCTT CCGGAGTCTG 
AATCCACCTC TGACAATTGT CCGGAAGACC TTCGAGTCCA CGGAGAACCC CGACGACTTC 
TTGCCCTCTG TAATGACTTG TGTGAACTAC CTTAAGTTGC CGGACTACTC GAGCATTGAC 
ATCATGCGTG AGAAACTGTT GATTGCAGCG AGAGAAGGGC AGCAGTCATT CCATCTTTCC 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_022265382.1
CDS	1..6063
Translation	MSNRPNNNPGGSLRRSQRNTAGAQPQDDSIGGRSCSSSSAVIVPQPEDPDRANTSERQKTGQVPKKDNSRGVKRSASPDYNRTNSPSSAKKPKALQHTESPSETNKPHSKSKKRHLDQEQQLKSAQSPSTSKAHTRKSGATGGSRSQKRKRTESSCLKSGSVSESTGAEERSAKPTKLASKSAASAKAGCSTITDSSSAASTSSSSSAVASASSTVPPGARVKQGKDQSKARRSRSASSPSPRRSSREKEQSKTGGSSKFDWAARFSPKVSLPKTKLSLPGSSKSETSKPGPSGLQAKLASLRKSTKKRSESPPAELPSLRRSTRQKTTGSCASASRRGSGLGKRGAAEARRQEKMADPESSQETVNSSAARTDETPQGAAASSSVAGAVGMTTSGESESDDSEMGRLQALLEARGLPPHLFGPLGPRMSQLFHRTIGSGASSKAQQLLQGLQASDESQQLQAVIEMCQLLVMGNEETLGGFPVKSVVPALITLLQMEHNFDIMNHACRALTYMMEALPRSSAVVVDAIPVFLEKLQVIQCIDVAEQALTALEMLSRRHSKAILQAGGLADCLLYLEFFSINAQRNALAIAANCCQSITPDEFHFVADSLPLLTQRLTHQDKKSVESTCLCFARLVDNFQHEENLLQQVASKDLLTNVQQLLVVTPPILSSGMFIMVVRMFSLMCSNCPTLAVQLMKQNIAETLHFLLCGASNGSCQEQIDLVPRSPQELYELTSLICELMPCLPKEGIFAVDTMLKKGNAQNTDGAIWQWRDDRGLWHPYNRIDSRIIEAAHQVGEDEISLSTLGRVYTIDFNSMQQINEDTGTARAIQRKPNPLANTNTSGYSELKKDDARAQLMKEDPELAKSFIKTLFGVLYEVYSSSAGPAVRHKCLRAILRIIYFADAELLKDVLKNHAVSSHIASMLSSQDLKIVVGALQMAEILMQKLPDIFSVYFRREGVMHQVKHLAESESLLTSPPKACTNGSGSLGSTTSVSSGTATAATNAAADLGSPSLQHSRDDSLDLSPQGRLSDVLKRKRLPKRGPRRPKYSPPRDDDKVDNQAKSPTTTQSPKSSFLASLNPKTWGRLSAQSNSNNIEPARTAGVSGLARAASKDTISNNREKIKGWIKEQAHKFVERYFSSENMDGSNPALNVLQRLCAATEQLNLQVDGGAECLVEIRSIVSESDVSSFEIQHSGFVKQLLLYLTSKSEKDAVSREIRLKRFLHVFFSSPLPGEEPIGRVEPVGNAPLLALVHKMNNCLSQMEQFPVKVHDFPSGNGTGGRGSQALKFFNTHQLKCQLQRHPDCANVKQWKGGPVKIDPLALVQAIERYLVVRGYGRVREDDEDSDDDGSDEEIDESLAAQFLNSGNVRHRLQFYIGEHLLPYNMTVYQAVRQFSIQAEDERESTDDESNPLGRAGIWTKTHTIWYKPVREDEESNKDCVGGKRGRAQTAPTKTSPRNAKKHDELWHDGVCPSVSNPLEVYLISSPPENITFEDPSLDVILLLRVLHAISRYWYYLYDNAMCKEIIPTSEFINSKLTAKANRQLQDPLVIMTGNIPTWLTELGKTCPFFFPFDTRQMLFYVTAFDRDRAMQRLLDTNPEINQSDSQDSRVAPRLDRKKRTVNREELLKQAESVMQDLGSSRAMLEIQYENEVGTGLGPTLEFYALVSQELQRADLGLWRGEEVTLSNPKGSQEGTKYIQNLQGLFALPFGRTAKPAHIAKVKMKFRFLGKLMAKAIMDFRLVDLPLGLPFYKWMLRQETSLTSHDLFDIDPVVARSVYHLEDIVRQKKRLEQDKSQTKESLQYALETLTMNGCSVEDLGLDFTLPGFPNIELKKGGKDIPVTIHNLEEYLRLVIFWALNEGVSRQFDSFRDGFESVFPLSHLQYFYPEELDQLLCGSKADTWDAKTLMECCRPDHGYTHDSRAVKFLFEILSSFDNEQQRLFLQFVTGSPRLPVGGFRSLNPPLTIVRKTFESTENPDDFLPSVMTCVNYLKLPDYSSIDIMREKLLIAAREGQQSFHLS

Target ORF information:

RefSeq Version	XM_022409674.1
Organism	Canis lupus familiaris(dog)
Definition	Canis lupus familiaris thyroid hormone receptor interactor 12 (TRIP12), transcript variant X14, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_022409674.1

ORF Insert Sequence:

ATGTCCAACC GGCCTAATAA CAATCCAGGG GGGTCACTGC GACGTTCACA GAGGAACACT 
GCCGGGGCCC AACCACAAGA CGACTCGATA GGAGGAAGAA GCTGCAGTTC ATCATCTGCT 
GTCATAGTTC CACAACCAGA GGATCCAGAC AGAGCCAATA CTTCAGAAAG GCAAAAAACG 
GGGCAGGTGC CTAAGAAAGA CAATTCTCGA GGAGTGAAAC GCAGTGCTAG TCCAGACTAC 
AACAGGACCA ATTCTCCTAG CTCTGCCAAA AAACCAAAAG CACTTCAGCA TACTGAATCT 
CCCTCAGAAA CAAATAAGCC ACATAGTAAG TCGAAGAAGA GACATTTAGA CCAGGAGCAA 
CAACTGAAAT CTGCACAGTC GCCATCAACA AGTAAGGCTC ACACCAGAAA GAGTGGGGCC 
ACCGGCGGCT CACGGAGTCA GAAAAGAAAG AGGACAGAGA GTTCTTGTCT AAAGAGTGGT 
TCTGTGTCTG AGTCAACTGG TGCCGAAGAG AGATCTGCAA AACCCACCAA GCTGGCTTCA 
AAATCCGCCG CCTCGGCCAA AGCTGGGTGC AGCACCATCA CTGATTCTTC CTCCGCTGCC 
TCTACTTCCT CCTCATCTTC TGCTGTCGCC TCGGCCTCCT CCACTGTACC ACCAGGGGCC 
AGGGTGAAAC AAGGAAAAGA CCAGAGCAAG GCCAGGCGTT CCCGTTCAGC ATCCAGTCCC 
AGTCCCAGAA GAAGTAGCAG GGAAAAGGAA CAGAGTAAAA CTGGTGGCTC TTCAAAATTC 
GATTGGGCTG CTCGTTTCAG CCCTAAAGTT AGCCTTCCTA AAACCAAACT GTCTCTTCCA 
GGGTCTTCTA AGTCAGAGAC ATCAAAACCT GGACCTTCTG GATTACAGGC CAAATTAGCA 
AGTTTAAGAA AATCTACCAA GAAGCGCAGT GAATCCCCAC CTGCTGAGCT CCCCAGTTTG 
AGGCGGAGCA CACGCCAAAA GACCACGGGC TCCTGTGCTA GTGCCAGTCG GCGAGGCTCT 
GGCCTGGGCA AAAGAGGAGC AGCTGAAGCT CGTCGACAGG AGAAGATGGC GGACCCTGAA 
AGCAGCCAGG AGACCGTGAA TTCTTCAGCT GCACGGACAG ACGAAACTCC CCAAGGAGCT 
GCAGCCTCTA GTTCTGTTGC AGGGGCTGTG GGCATGACCA CCTCTGGGGA GAGTGAATCC 
GATGATTCTG AGATGGGACG ACTACAAGCT TTGTTAGAGG CCAGGGGTCT TCCTCCTCAT 
CTGTTTGGCC CTCTCGGTCC TCGGATGTCA CAACTTTTCC ATAGAACAAT TGGAAGCGGA 
GCTAGCTCTA AGGCCCAGCA GCTTCTGCAA GGACTACAAG CAAGTGATGA AAGCCAGCAA 
CTCCAGGCAG TTATTGAAAT GTGTCAGTTA TTGGTTATGG GAAATGAGGA GACGCTGGGA 
GGGTTTCCGG TCAAGAGTGT CGTTCCAGCT TTGATAACAT TACTGCAGAT GGAGCATAAT 
TTCGATATTA TGAACCATGC TTGTCGAGCC TTAACATATA TGATGGAAGC ACTTCCTCGG 
TCATCTGCTG TTGTAGTAGA TGCAATTCCT GTCTTTTTAG AAAAGCTGCA AGTTATTCAG 
TGTATTGATG TCGCAGAGCA GGCCCTGACT GCCTTGGAGA TGTTATCACG CAGACACAGT 
AAAGCCATTC TACAGGCGGG TGGTTTGGCA GACTGCTTGC TGTATCTAGA ATTCTTCAGC 
ATAAATGCCC AAAGAAATGC ACTAGCAATT GCAGCCAATT GCTGCCAGAG TATCACGCCA 
GATGAATTTC ATTTTGTGGC AGATTCCCTC CCGTTGCTTA CCCAAAGGCT AACCCATCAG 
GACAAAAAGT CAGTTGAAAG CACTTGCCTT TGTTTTGCAC GTCTAGTGGA CAACTTCCAA 
CATGAGGAGA ATTTACTCCA GCAGGTTGCC TCCAAAGATC TGCTTACGAA TGTTCAACAA 
CTCTTGGTAG TGACTCCACC CATTTTAAGT TCTGGGATGT TTATAATGGT GGTTCGCATG 
TTTTCTCTGA TGTGTTCCAA CTGTCCAACT TTAGCTGTTC AACTTATGAA GCAAAACATT 
GCAGAAACAC TTCACTTTCT CCTGTGTGGT GCCTCTAATG GAAGTTGTCA GGAACAGATT 
GATCTTGTTC CACGAAGTCC ACAAGAACTG TATGAACTGA CATCTCTGAT TTGCGAACTT 
ATGCCATGTT TACCAAAAGA AGGCATTTTT GCAGTTGATA CCATGCTGAA GAAGGGAAAT 
GCGCAGAACA CAGACGGTGC GATTTGGCAG TGGCGTGATG ACCGTGGCCT TTGGCATCCC 
TATAACAGGA TTGACAGCCG GATCATTGAG GCAGCCCATC AGGTCGGTGA GGATGAGATA 
AGCTTGTCCA CTCTGGGACG AGTTTATACT ATTGATTTTA ATTCTATGCA GCAAATCAAT 
GAGGACACGG GAACAGCACG TGCCATTCAG AGAAAACCTA ACCCCTTAGC CAATACTAAC 
ACTAGTGGAT ATTCAGAGTT AAAGAAGGAT GATGCTCGAG CACAGCTTAT GAAAGAGGAT 
CCGGAACTGG CTAAGTCTTT TATTAAGACA TTATTTGGTG TTCTTTATGA AGTGTATAGT 
TCCTCAGCAG GACCTGCGGT CAGACATAAG TGCCTTAGAG CAATTCTTAG GATAATTTAT 
TTTGCGGATG CTGAACTTCT GAAGGATGTC CTGAAAAATC ATGCTGTTTC AAGTCACATT 
GCTTCCATGC TGTCAAGCCA AGACCTGAAG ATAGTAGTTG GAGCACTTCA GATGGCAGAA 
ATCTTAATGC AGAAGTTACC TGATATTTTT AGTGTTTACT TCAGAAGAGA AGGTGTAATG 
CATCAAGTAA AACACTTAGC AGAATCAGAG TCTTTGTTGA CAAGTCCACC AAAGGCATGT 
ACGAATGGGT CAGGATCCCT GGGATCCACG ACGTCAGTCA GCAGTGGAAC AGCCACGGCC 
GCCACCAATG CTGCAGCTGA CCTGGGGTCC CCCAGCTTGC AGCACAGCAG AGATGACTCT 
TTAGATCTGA GCCCTCAAGG TCGATTAAGT GATGTTCTAA AGAGAAAACG GCTGCCAAAA 
CGAGGGCCAA GAAGGCCAAA GTATTCACCT CCAAGAGATG ATGACAAAGT AGACAATCAA 
GCTAAAAGCC CTACCACTAC TCAGTCACCT AAATCTTCTT TCCTGGCAAG CTTGAATCCA 
AAAACGTGGG GAAGGTTAAG CGCACAGTCC AACAGCAACA ACATCGAACC AGCACGAACT 
GCGGGAGTCA GTGGTCTTGC CAGGGCCGCC TCCAAGGACA CCATATCCAA TAACAGAGAA 
AAAATTAAAG GTTGGATTAA GGAACAGGCG CATAAATTTG TAGAGCGTTA TTTCAGTTCT 
GAGAACATGG ATGGAAGCAA CCCTGCACTG AATGTCCTTC AGAGACTTTG TGCTGCAACT 
GAACAACTCA ACCTCCAGGT GGATGGTGGA GCTGAGTGCC TTGTAGAAAT CCGTAGCATA 
GTCTCAGAGT CTGATGTTTC ATCATTTGAA ATCCAACATA GTGGATTTGT GAAGCAGCTG 
TTACTTTATT TGACATCTAA AAGTGAAAAG GATGCTGTGA GCCGAGAGAT CAGATTAAAG 
AGATTCCTTC ACGTATTTTT TTCTTCACCA CTTCCTGGAG AAGAGCCCAT TGGAAGAGTA 
GAACCAGTGG GTAATGCGCC TTTGTTGGCA TTAGTTCACA AGATGAACAA CTGCCTCAGC 
CAGATGGAAC AGTTTCCAGT CAAAGTACAT GACTTCCCCA GTGGGAACGG GACAGGAGGC 
AGAGGATCAC AGGCTTTAAA ATTTTTCAAC ACACACCAGT TAAAATGCCA GTTACAAAGA 
CATCCAGACT GTGCAAATGT GAAGCAGTGG AAGGGCGGGC CGGTCAAGAT TGATCCCCTG 
GCTTTGGTAC AAGCTATTGA GAGATACCTT GTAGTTAGAG GGTATGGAAG AGTAAGAGAA 
GATGATGAAG ACAGTGATGA TGATGGCTCT GATGAGGAAA TAGACGAGTC TCTGGCCGCT 
CAGTTCTTAA ATTCCGGAAA CGTAAGGCAC AGGCTACAGT TTTATATTGG AGAACATTTG 
CTACCATATA ACATGACTGT ATATCAGGCA GTCCGGCAGT TCAGTATACA AGCTGAGGAT 
GAGCGGGAGT CCACAGATGA TGAGAGCAAC CCTCTGGGAA GAGCTGGTAT TTGGACAAAG 
ACTCATACAA TATGGTACAA ACCTGTGAGA GAGGATGAAG AAAGTAATAA AGATTGTGTT 
GGTGGTAAAA GAGGAAGAGC CCAAACGGCT CCAACAAAAA CATCCCCTAG AAATGCAAAA 
AAGCATGATG AGTTATGGCA TGATGGAGTA TGCCCATCAG TATCCAATCC TTTAGAAGTT 
TACCTCATCT CCTCGCCACC TGAGAATATA ACTTTTGAAG ACCCGTCCTT AGATGTGATT 
CTTCTTCTAA GAGTTTTACA TGCCATCAGC CGATACTGGT ATTACTTGTA TGATAATGCA 
ATGTGCAAGG AGATTATTCC AACTAGTGAA TTTATTAACA GTAAGTTGAC AGCAAAAGCA 
AATAGGCAGC TTCAAGATCC TTTAGTAATC ATGACAGGAA ATATCCCAAC GTGGCTTACT 
GAACTAGGAA AAACCTGCCC ATTTTTCTTC CCTTTTGATA CCCGGCAAAT GCTTTTTTAC 
GTAACTGCAT TTGATCGGGA CCGAGCAATG CAAAGATTAC TTGATACCAA CCCAGAAATC 
AACCAGTCGG ATTCTCAAGA TAGCAGAGTT GCACCTAGAT TGGATAGAAA AAAACGTACT 
GTGAACAGAG AGGAGCTGCT GAAACAAGCG GAGTCTGTAA TGCAGGATCT CGGTAGCTCA 
CGGGCCATGT TAGAAATCCA GTATGAAAAT GAGGTTGGTA CAGGTCTTGG GCCTACACTG 
GAGTTTTATG CACTTGTATC TCAGGAACTT CAGAGAGCTG ACTTGGGTCT CTGGAGAGGT 
GAAGAAGTGA CTCTCAGCAA TCCAAAAGGA AGCCAAGAAG GGACCAAGTA TATTCAAAAC 
CTCCAGGGCC TGTTTGCACT TCCCTTTGGT AGGACAGCTA AGCCAGCCCA TATCGCAAAG 
GTTAAAATGA AGTTTCGCTT CTTAGGAAAA TTAATGGCCA AGGCTATCAT GGATTTCAGA 
TTGGTGGACC TTCCCCTTGG TTTACCCTTT TATAAGTGGA TGCTAAGGCA AGAAACTTCA 
CTGACGTCGC ATGATTTGTT TGACATTGAC CCAGTTGTAG CCAGATCAGT GTATCACCTA 
GAAGACATTG TCAGACAGAA GAAAAGACTT GAGCAAGATA AATCCCAGAC CAAAGAGAGT 
CTACAATATG CATTAGAAAC CCTGACTATG AATGGCTGCT CAGTTGAAGA TCTAGGATTG 
GATTTCACGC TGCCAGGGTT TCCCAACATT GAACTGAAGA AAGGAGGAAA GGATATACCA 
GTTACCATCC ACAATTTAGA GGAGTATCTA AGACTGGTTA TATTCTGGGC ACTAAATGAA 
GGCGTTTCCA GGCAATTTGA TTCCTTCAGA GATGGATTTG AATCTGTCTT CCCACTTAGT 
CATCTTCAGT ACTTCTACCC GGAAGAACTG GATCAGCTCT TGTGCGGCAG TAAAGCAGAC 
ACGTGGGATG CGAAGACACT GATGGAGTGC TGCAGGCCGG ACCACGGTTA TACTCATGAC 
AGTCGGGCTG TGAAGTTCTT GTTTGAGATT CTCAGTAGTT TTGATAATGA GCAGCAGAGG 
TTGTTTCTCC AGTTTGTGAC AGGTAGCCCA AGATTGCCTG TTGGAGGCTT CCGGAGTCTG 
AATCCACCTC TGACAATTGT CCGGAAGACC TTCGAGTCCA CGGAGAACCC CGACGACTTC 
TTGCCCTCTG TAATGACTTG TGTGAACTAC CTTAAGTTGC CGGACTACTC GAGCATTGAC 
ATCATGCGTG AGAAACTGTT GATTGCAGCG AGAGAAGGGC AGCAGTCATT CCATCTTTCC 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TRIP12 ( XM_005635800.3 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_005635857.1 Canis lupus familiaris thyroid hormone receptor interactor 12 (TRIP12), transcript variant X1, mRNA.

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CloneID	OCe25761
Clone ID Related Accession (Same CDS sequence)	XM_005635800.3 , XM_005635801.2 , XM_005635800.2 , XM_005635801.3 , XM_022409663.1
Accession Version	XM_005635800.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6207bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-09-04
Organism	Canis lupus familiaris(dog)
Product	E3 ubiquitin-protein ligase TRIP12 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006607.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 5, 2017 this sequence version replaced XM_005635800.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCCAACC GGCCTAATAA CAATCCAGGG GGGTCACTGC GACGTTCACA GAGGAACACT 
GCCGGGGCCC AACCACAAGA CGACTCGATA GGAGGAAGGT CACATTTAGG GCAGGCAAAA 
CATAAGGGAT ATAGCCCTCC TGAGAGTAGA AAATCTAATT CTAAGGCACC CAAAGTGCAG 
TCTAATACTA CTTCTGAACT GTCAAGGGGA CACCTTTCTA AAAGAAGCTG CAGTTCATCA 
TCTGCTGTCA TAGTTCCACA ACCAGAGGAT CCAGACAGAG CCAATACTTC AGAAAGGCAA 
AAAACGGGGC AGGTGCCTAA GAAAGACAAT TCTCGAGGAG TGAAACGCAG TGCTAGTCCA 
GACTACAACA GGACCAATTC TCCTAGCTCT GCCAAAAAAC CAAAAGCACT TCAGCATACT 
GAATCTCCCT CAGAAACAAA TAAGCCACAT AGTAAGTCGA AGAAGAGACA TTTAGACCAG 
GAGCAACAAC TGAAATCTGC ACAGTCGCCA TCAACAAGTA AGGCTCACAC CAGAAAGAGT 
GGGGCCACCG GCGGCTCACG GAGTCAGAAA AGAAAGAGGA CAGAGAGTTC TTGTCTAAAG 
AGTGGTTCTG TGTCTGAGTC AACTGGTGCC GAAGAGAGAT CTGCAAAACC CACCAAGCTG 
GCTTCAAAAT CCGCCGCCTC GGCCAAAGCT GGGTGCAGCA CCATCACTGA TTCTTCCTCC 
GCTGCCTCTA CTTCCTCCTC ATCTTCTGCT GTCGCCTCGG CCTCCTCCAC TGTACCACCA 
GGGGCCAGGG TGAAACAAGG AAAAGACCAG AGCAAGGCCA GGCGTTCCCG TTCAGCATCC 
AGTCCCAGTC CCAGAAGAAG TAGCAGGGAA AAGGAACAGA GTAAAACTGG TGGCTCTTCA 
AAATTCGATT GGGCTGCTCG TTTCAGCCCT AAAGTTAGCC TTCCTAAAAC CAAACTGTCT 
CTTCCAGGGT CTTCTAAGTC AGAGACATCA AAACCTGGAC CTTCTGGATT ACAGGCCAAA 
TTAGCAAGTT TAAGAAAATC TACCAAGAAG CGCAGTGAAT CCCCACCTGC TGAGCTCCCC 
AGTTTGAGGC GGAGCACACG CCAAAAGACC ACGGGCTCCT GTGCTAGTGC CAGTCGGCGA 
GGCTCTGGCC TGGGCAAAAG AGGAGCAGCT GAAGCTCGTC GACAGGAGAA GATGGCGGAC 
CCTGAAAGCA GCCAGGAGAC CGTGAATTCT TCAGCTGCAC GGACAGACGA AACTCCCCAA 
GGAGCTGCAG CCTCTAGTTC TGTTGCAGGG GCTGTGGGCA TGACCACCTC TGGGGAGAGT 
GAATCCGATG ATTCTGAGAT GGGACGACTA CAAGCTTTGT TAGAGGCCAG GGGTCTTCCT 
CCTCATCTGT TTGGCCCTCT CGGTCCTCGG ATGTCACAAC TTTTCCATAG AACAATTGGA 
AGCGGAGCTA GCTCTAAGGC CCAGCAGCTT CTGCAAGGAC TACAAGCAAG TGATGAAAGC 
CAGCAACTCC AGGCAGTTAT TGAAATGTGT CAGTTATTGG TTATGGGAAA TGAGGAGACG 
CTGGGAGGGT TTCCGGTCAA GAGTGTCGTT CCAGCTTTGA TAACATTACT GCAGATGGAG 
CATAATTTCG ATATTATGAA CCATGCTTGT CGAGCCTTAA CATATATGAT GGAAGCACTT 
CCTCGGTCAT CTGCTGTTGT AGTAGATGCA ATTCCTGTCT TTTTAGAAAA GCTGCAAGTT 
ATTCAGTGTA TTGATGTCGC AGAGCAGGCC CTGACTGCCT TGGAGATGTT ATCACGCAGA 
CACAGTAAAG CCATTCTACA GGCGGGTGGT TTGGCAGACT GCTTGCTGTA TCTAGAATTC 
TTCAGCATAA ATGCCCAAAG AAATGCACTA GCAATTGCAG CCAATTGCTG CCAGAGTATC 
ACGCCAGATG AATTTCATTT TGTGGCAGAT TCCCTCCCGT TGCTTACCCA AAGGCTAACC 
CATCAGGACA AAAAGTCAGT TGAAAGCACT TGCCTTTGTT TTGCACGTCT AGTGGACAAC 
TTCCAACATG AGGAGAATTT ACTCCAGCAG GTTGCCTCCA AAGATCTGCT TACGAATGTT 
CAACAACTCT TGGTAGTGAC TCCACCCATT TTAAGTTCTG GGATGTTTAT AATGGTGGTT 
CGCATGTTTT CTCTGATGTG TTCCAACTGT CCAACTTTAG CTGTTCAACT TATGAAGCAA 
AACATTGCAG AAACACTTCA CTTTCTCCTG TGTGGTGCCT CTAATGGAAG TTGTCAGGAA 
CAGATTGATC TTGTTCCACG AAGTCCACAA GAACTGTATG AACTGACATC TCTGATTTGC 
GAACTTATGC CATGTTTACC AAAAGAAGGC ATTTTTGCAG TTGATACCAT GCTGAAGAAG 
GGAAATGCGC AGAACACAGA CGGTGCGATT TGGCAGTGGC GTGATGACCG TGGCCTTTGG 
CATCCCTATA ACAGGATTGA CAGCCGGATC ATTGAGGTAG CAGCCCATCA GGTCGGTGAG 
GATGAGATAA GCTTGTCCAC TCTGGGACGA GTTTATACTA TTGATTTTAA TTCTATGCAG 
CAAATCAATG AGGACACGGG AACAGCACGT GCCATTCAGA GAAAACCTAA CCCCTTAGCC 
AATACTAACA CTAGTGGATA TTCAGAGTTA AAGAAGGATG ATGCTCGAGC ACAGCTTATG 
AAAGAGGATC CGGAACTGGC TAAGTCTTTT ATTAAGACAT TATTTGGTGT TCTTTATGAA 
GTGTATAGTT CCTCAGCAGG ACCTGCGGTC AGACATAAGT GCCTTAGAGC AATTCTTAGG 
ATAATTTATT TTGCGGATGC TGAACTTCTG AAGGATGTCC TGAAAAATCA TGCTGTTTCA 
AGTCACATTG CTTCCATGCT GTCAAGCCAA GACCTGAAGA TAGTAGTTGG AGCACTTCAG 
ATGGCAGAAA TCTTAATGCA GAAGTTACCT GATATTTTTA GTGTTTACTT CAGAAGAGAA 
GGTGTAATGC ATCAAGTAAA ACACTTAGCA GAATCAGAGT CTTTGTTGAC AAGTCCACCA 
AAGGCATGTA CGAATGGGTC AGGATCCCTG GGATCCACGA CGTCAGTCAG CAGTGGAACA 
GCCACGGCCG CCACCAATGC TGCAGCTGAC CTGGGGTCCC CCAGCTTGCA GCACAGCAGA 
GATGACTCTT TAGATCTGAG CCCTCAAGGT CGATTAAGTG ATGTTCTAAA GAGAAAACGG 
CTGCCAAAAC GAGGGCCAAG AAGGCCAAAG TATTCACCTC CAAGAGATGA TGACAAAGTA 
GACAATCAAG CTAAAAGCCC TACCACTACT CAGTCACCTA AATCTTCTTT CCTGGCAAGC 
TTGAATCCAA AAACGTGGGG AAGGTTAAGC GCACAGTCCA ACAGCAACAA CATCGAACCA 
GCACGAACTG CGGGAGTCAG TGGTCTTGCC AGGGCCGCCT CCAAGGACAC CATATCCAAT 
AACAGAGAAA AAATTAAAGG TTGGATTAAG GAACAGGCGC ATAAATTTGT AGAGCGTTAT 
TTCAGTTCTG AGAACATGGA TGGAAGCAAC CCTGCACTGA ATGTCCTTCA GAGACTTTGT 
GCTGCAACTG AACAACTCAA CCTCCAGGTG GATGGTGGAG CTGAGTGCCT TGTAGAAATC 
CGTAGCATAG TCTCAGAGTC TGATGTTTCA TCATTTGAAA TCCAACATAG TGGATTTGTG 
AAGCAGCTGT TACTTTATTT GACATCTAAA AGTGAAAAGG ATGCTGTGAG CCGAGAGATC 
AGATTAAAGA GATTCCTTCA CGTATTTTTT TCTTCACCAC TTCCTGGAGA AGAGCCCATT 
GGAAGAGTAG AACCAGTGGG TAATGCGCCT TTGTTGGCAT TAGTTCACAA GATGAACAAC 
TGCCTCAGCC AGATGGAACA GTTTCCAGTC AAAGTACATG ACTTCCCCAG TGGGAACGGG 
ACAGGAGGCA GCTTTTCTCT CAACAGAGGA TCACAGGCTT TAAAATTTTT CAACACACAC 
CAGTTAAAAT GCCAGTTACA AAGACATCCA GACTGTGCAA ATGTGAAGCA GTGGAAGGGC 
GGGCCGGTCA AGATTGATCC CCTGGCTTTG GTACAAGCTA TTGAGAGATA CCTTGTAGTT 
AGAGGGTATG GAAGAGTAAG AGAAGATGAT GAAGACAGTG ATGATGATGG CTCTGATGAG 
GAAATAGACG AGTCTCTGGC CGCTCAGTTC TTAAATTCCG GAAACGTAAG GCACAGGCTA 
CAGTTTTATA TTGGAGAACA TTTGCTACCA TATAACATGA CTGTATATCA GGCAGTCCGG 
CAGTTCAGTA TACAAGCTGA GGATGAGCGG GAGTCCACAG ATGATGAGAG CAACCCTCTG 
GGAAGAGCTG GTATTTGGAC AAAGACTCAT ACAATATGGT ACAAACCTGT GAGAGAGGAT 
GAAGAAAGTA ATAAAGATTG TGTTGGTGGT AAAAGAGGAA GAGCCCAAAC GGCTCCAACA 
AAAACATCCC CTAGAAATGC AAAAAAGCAT GATGAGTTAT GGCATGATGG AGTATGCCCA 
TCAGTATCCA ATCCTTTAGA AGTTTACCTC ATCTCCTCGC CACCTGAGAA TATAACTTTT 
GAAGACCCGT CCTTAGATGT GATTCTTCTT CTAAGAGTTT TACATGCCAT CAGCCGATAC 
TGGTATTACT TGTATGATAA TGCAATGTGC AAGGAGATTA TTCCAACTAG TGAATTTATT 
AACAGTAAGT TGACAGCAAA AGCAAATAGG CAGCTTCAAG ATCCTTTAGT AATCATGACA 
GGAAATATCC CAACGTGGCT TACTGAACTA GGAAAAACCT GCCCATTTTT CTTCCCTTTT 
GATACCCGGC AAATGCTTTT TTACGTAACT GCATTTGATC GGGACCGAGC AATGCAAAGA 
TTACTTGATA CCAACCCAGA AATCAACCAG TCGGATTCTC AAGATAGCAG AGTTGCACCT 
AGATTGGATA GAAAAAAACG TACTGTGAAC AGAGAGGAGC TGCTGAAACA AGCGGAGTCT 
GTAATGCAGG ATCTCGGTAG CTCACGGGCC ATGTTAGAAA TCCAGTATGA AAATGAGGTT 
GGTACAGGTC TTGGGCCTAC ACTGGAGTTT TATGCACTTG TATCTCAGGA ACTTCAGAGA 
GCTGACTTGG GTCTCTGGAG AGGTGAAGAA GTGACTCTCA GCAATCCAAA AGGAAGCCAA 
GAAGGGACCA AGTATATTCA AAACCTCCAG GGCCTGTTTG CACTTCCCTT TGGTAGGACA 
GCTAAGCCAG CCCATATCGC AAAGGTTAAA ATGAAGTTTC GCTTCTTAGG AAAATTAATG 
GCCAAGGCTA TCATGGATTT CAGATTGGTG GACCTTCCCC TTGGTTTACC CTTTTATAAG 
TGGATGCTAA GGCAAGAAAC TTCACTGACG TCGCATGATT TGTTTGACAT TGACCCAGTT 
GTAGCCAGAT CAGTGTATCA CCTAGAAGAC ATTGTCAGAC AGAAGAAAAG ACTTGAGCAA 
GATAAATCCC AGACCAAAGA GAGTCTACAA TATGCATTAG AAACCCTGAC TATGAATGGC 
TGCTCAGTTG AAGATCTAGG ATTGGATTTC ACGCTGCCAG GGTTTCCCAA CATTGAACTG 
AAGAAAGGAG GAAAGGATAT ACCAGTTACC ATCCACAATT TAGAGGAGTA TCTAAGACTG 
GTTATATTCT GGGCACTAAA TGAAGGCGTT TCCAGGCAAT TTGATTCCTT CAGAGATGGA 
TTTGAATCTG TCTTCCCACT TAGTCATCTT CAGTACTTCT ACCCGGAAGA ACTGGATCAG 
CTCTTGTGCG GCAGTAAAGC AGACACGTGG GATGCGAAGA CACTGATGGA GTGCTGCAGG 
CCGGACCACG GTTATACTCA TGACAGTCGG GCTGTGAAGT TCTTGTTTGA GATTCTCAGT 
AGTTTTGATA ATGAGCAGCA GAGGTTGTTT CTCCAGTTTG TGACAGGTAG CCCAAGATTG 
CCTGTTGGAG GCTTCCGGAG TCTGAATCCA CCTCTGACAA TTGTCCGGAA GACCTTCGAG 
TCCACGGAGA ACCCCGACGA CTTCTTGCCC TCTGTAATGA CTTGTGTGAA CTACCTTAAG 
TTGCCGGACT ACTCGAGCAT TGACATCATG CGTGAGAAAC TGTTGATTGC AGCGAGAGAA 
GGGCAGCAGT CATTCCATCT TTCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005635857.1
CDS	162..6368
Translation	MSNRPNNNPGGSLRRSQRNTAGAQPQDDSIGGRSHLGQAKHKGYSPPESRKSNSKAPKVQSNTTSELSRGHLSKRSCSSSSAVIVPQPEDPDRANTSERQKTGQVPKKDNSRGVKRSASPDYNRTNSPSSAKKPKALQHTESPSETNKPHSKSKKRHLDQEQQLKSAQSPSTSKAHTRKSGATGGSRSQKRKRTESSCLKSGSVSESTGAEERSAKPTKLASKSAASAKAGCSTITDSSSAASTSSSSSAVASASSTVPPGARVKQGKDQSKARRSRSASSPSPRRSSREKEQSKTGGSSKFDWAARFSPKVSLPKTKLSLPGSSKSETSKPGPSGLQAKLASLRKSTKKRSESPPAELPSLRRSTRQKTTGSCASASRRGSGLGKRGAAEARRQEKMADPESSQETVNSSAARTDETPQGAAASSSVAGAVGMTTSGESESDDSEMGRLQALLEARGLPPHLFGPLGPRMSQLFHRTIGSGASSKAQQLLQGLQASDESQQLQAVIEMCQLLVMGNEETLGGFPVKSVVPALITLLQMEHNFDIMNHACRALTYMMEALPRSSAVVVDAIPVFLEKLQVIQCIDVAEQALTALEMLSRRHSKAILQAGGLADCLLYLEFFSINAQRNALAIAANCCQSITPDEFHFVADSLPLLTQRLTHQDKKSVESTCLCFARLVDNFQHEENLLQQVASKDLLTNVQQLLVVTPPILSSGMFIMVVRMFSLMCSNCPTLAVQLMKQNIAETLHFLLCGASNGSCQEQIDLVPRSPQELYELTSLICELMPCLPKEGIFAVDTMLKKGNAQNTDGAIWQWRDDRGLWHPYNRIDSRIIEVAAHQVGEDEISLSTLGRVYTIDFNSMQQINEDTGTARAIQRKPNPLANTNTSGYSELKKDDARAQLMKEDPELAKSFIKTLFGVLYEVYSSSAGPAVRHKCLRAILRIIYFADAELLKDVLKNHAVSSHIASMLSSQDLKIVVGALQMAEILMQKLPDIFSVYFRREGVMHQVKHLAESESLLTSPPKACTNGSGSLGSTTSVSSGTATAATNAAADLGSPSLQHSRDDSLDLSPQGRLSDVLKRKRLPKRGPRRPKYSPPRDDDKVDNQAKSPTTTQSPKSSFLASLNPKTWGRLSAQSNSNNIEPARTAGVSGLARAASKDTISNNREKIKGWIKEQAHKFVERYFSSENMDGSNPALNVLQRLCAATEQLNLQVDGGAECLVEIRSIVSESDVSSFEIQHSGFVKQLLLYLTSKSEKDAVSREIRLKRFLHVFFSSPLPGEEPIGRVEPVGNAPLLALVHKMNNCLSQMEQFPVKVHDFPSGNGTGGSFSLNRGSQALKFFNTHQLKCQLQRHPDCANVKQWKGGPVKIDPLALVQAIERYLVVRGYGRVREDDEDSDDDGSDEEIDESLAAQFLNSGNVRHRLQFYIGEHLLPYNMTVYQAVRQFSIQAEDERESTDDESNPLGRAGIWTKTHTIWYKPVREDEESNKDCVGGKRGRAQTAPTKTSPRNAKKHDELWHDGVCPSVSNPLEVYLISSPPENITFEDPSLDVILLLRVLHAISRYWYYLYDNAMCKEIIPTSEFINSKLTAKANRQLQDPLVIMTGNIPTWLTELGKTCPFFFPFDTRQMLFYVTAFDRDRAMQRLLDTNPEINQSDSQDSRVAPRLDRKKRTVNREELLKQAESVMQDLGSSRAMLEIQYENEVGTGLGPTLEFYALVSQELQRADLGLWRGEEVTLSNPKGSQEGTKYIQNLQGLFALPFGRTAKPAHIAKVKMKFRFLGKLMAKAIMDFRLVDLPLGLPFYKWMLRQETSLTSHDLFDIDPVVARSVYHLEDIVRQKKRLEQDKSQTKESLQYALETLTMNGCSVEDLGLDFTLPGFPNIELKKGGKDIPVTIHNLEEYLRLVIFWALNEGVSRQFDSFRDGFESVFPLSHLQYFYPEELDQLLCGSKADTWDAKTLMECCRPDHGYTHDSRAVKFLFEILSSFDNEQQRLFLQFVTGSPRLPVGGFRSLNPPLTIVRKTFESTENPDDFLPSVMTCVNYLKLPDYSSIDIMREKLLIAAREGQQSFHLS

Target ORF information:

RefSeq Version	XM_005635800.3
Organism	Canis lupus familiaris(dog)
Definition	Canis lupus familiaris thyroid hormone receptor interactor 12 (TRIP12), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005635800.3

ORF Insert Sequence:

ATGTCCAACC GGCCTAATAA CAATCCAGGG GGGTCACTGC GACGTTCACA GAGGAACACT 
GCCGGGGCCC AACCACAAGA CGACTCGATA GGAGGAAGGT CACATTTAGG GCAGGCAAAA 
CATAAGGGAT ATAGCCCTCC TGAGAGTAGA AAATCTAATT CTAAGGCACC CAAAGTGCAG 
TCTAATACTA CTTCTGAACT GTCAAGGGGA CACCTTTCTA AAAGAAGCTG CAGTTCATCA 
TCTGCTGTCA TAGTTCCACA ACCAGAGGAT CCAGACAGAG CCAATACTTC AGAAAGGCAA 
AAAACGGGGC AGGTGCCTAA GAAAGACAAT TCTCGAGGAG TGAAACGCAG TGCTAGTCCA 
GACTACAACA GGACCAATTC TCCTAGCTCT GCCAAAAAAC CAAAAGCACT TCAGCATACT 
GAATCTCCCT CAGAAACAAA TAAGCCACAT AGTAAGTCGA AGAAGAGACA TTTAGACCAG 
GAGCAACAAC TGAAATCTGC ACAGTCGCCA TCAACAAGTA AGGCTCACAC CAGAAAGAGT 
GGGGCCACCG GCGGCTCACG GAGTCAGAAA AGAAAGAGGA CAGAGAGTTC TTGTCTAAAG 
AGTGGTTCTG TGTCTGAGTC AACTGGTGCC GAAGAGAGAT CTGCAAAACC CACCAAGCTG 
GCTTCAAAAT CCGCCGCCTC GGCCAAAGCT GGGTGCAGCA CCATCACTGA TTCTTCCTCC 
GCTGCCTCTA CTTCCTCCTC ATCTTCTGCT GTCGCCTCGG CCTCCTCCAC TGTACCACCA 
GGGGCCAGGG TGAAACAAGG AAAAGACCAG AGCAAGGCCA GGCGTTCCCG TTCAGCATCC 
AGTCCCAGTC CCAGAAGAAG TAGCAGGGAA AAGGAACAGA GTAAAACTGG TGGCTCTTCA 
AAATTCGATT GGGCTGCTCG TTTCAGCCCT AAAGTTAGCC TTCCTAAAAC CAAACTGTCT 
CTTCCAGGGT CTTCTAAGTC AGAGACATCA AAACCTGGAC CTTCTGGATT ACAGGCCAAA 
TTAGCAAGTT TAAGAAAATC TACCAAGAAG CGCAGTGAAT CCCCACCTGC TGAGCTCCCC 
AGTTTGAGGC GGAGCACACG CCAAAAGACC ACGGGCTCCT GTGCTAGTGC CAGTCGGCGA 
GGCTCTGGCC TGGGCAAAAG AGGAGCAGCT GAAGCTCGTC GACAGGAGAA GATGGCGGAC 
CCTGAAAGCA GCCAGGAGAC CGTGAATTCT TCAGCTGCAC GGACAGACGA AACTCCCCAA 
GGAGCTGCAG CCTCTAGTTC TGTTGCAGGG GCTGTGGGCA TGACCACCTC TGGGGAGAGT 
GAATCCGATG ATTCTGAGAT GGGACGACTA CAAGCTTTGT TAGAGGCCAG GGGTCTTCCT 
CCTCATCTGT TTGGCCCTCT CGGTCCTCGG ATGTCACAAC TTTTCCATAG AACAATTGGA 
AGCGGAGCTA GCTCTAAGGC CCAGCAGCTT CTGCAAGGAC TACAAGCAAG TGATGAAAGC 
CAGCAACTCC AGGCAGTTAT TGAAATGTGT CAGTTATTGG TTATGGGAAA TGAGGAGACG 
CTGGGAGGGT TTCCGGTCAA GAGTGTCGTT CCAGCTTTGA TAACATTACT GCAGATGGAG 
CATAATTTCG ATATTATGAA CCATGCTTGT CGAGCCTTAA CATATATGAT GGAAGCACTT 
CCTCGGTCAT CTGCTGTTGT AGTAGATGCA ATTCCTGTCT TTTTAGAAAA GCTGCAAGTT 
ATTCAGTGTA TTGATGTCGC AGAGCAGGCC CTGACTGCCT TGGAGATGTT ATCACGCAGA 
CACAGTAAAG CCATTCTACA GGCGGGTGGT TTGGCAGACT GCTTGCTGTA TCTAGAATTC 
TTCAGCATAA ATGCCCAAAG AAATGCACTA GCAATTGCAG CCAATTGCTG CCAGAGTATC 
ACGCCAGATG AATTTCATTT TGTGGCAGAT TCCCTCCCGT TGCTTACCCA AAGGCTAACC 
CATCAGGACA AAAAGTCAGT TGAAAGCACT TGCCTTTGTT TTGCACGTCT AGTGGACAAC 
TTCCAACATG AGGAGAATTT ACTCCAGCAG GTTGCCTCCA AAGATCTGCT TACGAATGTT 
CAACAACTCT TGGTAGTGAC TCCACCCATT TTAAGTTCTG GGATGTTTAT AATGGTGGTT 
CGCATGTTTT CTCTGATGTG TTCCAACTGT CCAACTTTAG CTGTTCAACT TATGAAGCAA 
AACATTGCAG AAACACTTCA CTTTCTCCTG TGTGGTGCCT CTAATGGAAG TTGTCAGGAA 
CAGATTGATC TTGTTCCACG AAGTCCACAA GAACTGTATG AACTGACATC TCTGATTTGC 
GAACTTATGC CATGTTTACC AAAAGAAGGC ATTTTTGCAG TTGATACCAT GCTGAAGAAG 
GGAAATGCGC AGAACACAGA CGGTGCGATT TGGCAGTGGC GTGATGACCG TGGCCTTTGG 
CATCCCTATA ACAGGATTGA CAGCCGGATC ATTGAGGTAG CAGCCCATCA GGTCGGTGAG 
GATGAGATAA GCTTGTCCAC TCTGGGACGA GTTTATACTA TTGATTTTAA TTCTATGCAG 
CAAATCAATG AGGACACGGG AACAGCACGT GCCATTCAGA GAAAACCTAA CCCCTTAGCC 
AATACTAACA CTAGTGGATA TTCAGAGTTA AAGAAGGATG ATGCTCGAGC ACAGCTTATG 
AAAGAGGATC CGGAACTGGC TAAGTCTTTT ATTAAGACAT TATTTGGTGT TCTTTATGAA 
GTGTATAGTT CCTCAGCAGG ACCTGCGGTC AGACATAAGT GCCTTAGAGC AATTCTTAGG 
ATAATTTATT TTGCGGATGC TGAACTTCTG AAGGATGTCC TGAAAAATCA TGCTGTTTCA 
AGTCACATTG CTTCCATGCT GTCAAGCCAA GACCTGAAGA TAGTAGTTGG AGCACTTCAG 
ATGGCAGAAA TCTTAATGCA GAAGTTACCT GATATTTTTA GTGTTTACTT CAGAAGAGAA 
GGTGTAATGC ATCAAGTAAA ACACTTAGCA GAATCAGAGT CTTTGTTGAC AAGTCCACCA 
AAGGCATGTA CGAATGGGTC AGGATCCCTG GGATCCACGA CGTCAGTCAG CAGTGGAACA 
GCCACGGCCG CCACCAATGC TGCAGCTGAC CTGGGGTCCC CCAGCTTGCA GCACAGCAGA 
GATGACTCTT TAGATCTGAG CCCTCAAGGT CGATTAAGTG ATGTTCTAAA GAGAAAACGG 
CTGCCAAAAC GAGGGCCAAG AAGGCCAAAG TATTCACCTC CAAGAGATGA TGACAAAGTA 
GACAATCAAG CTAAAAGCCC TACCACTACT CAGTCACCTA AATCTTCTTT CCTGGCAAGC 
TTGAATCCAA AAACGTGGGG AAGGTTAAGC GCACAGTCCA ACAGCAACAA CATCGAACCA 
GCACGAACTG CGGGAGTCAG TGGTCTTGCC AGGGCCGCCT CCAAGGACAC CATATCCAAT 
AACAGAGAAA AAATTAAAGG TTGGATTAAG GAACAGGCGC ATAAATTTGT AGAGCGTTAT 
TTCAGTTCTG AGAACATGGA TGGAAGCAAC CCTGCACTGA ATGTCCTTCA GAGACTTTGT 
GCTGCAACTG AACAACTCAA CCTCCAGGTG GATGGTGGAG CTGAGTGCCT TGTAGAAATC 
CGTAGCATAG TCTCAGAGTC TGATGTTTCA TCATTTGAAA TCCAACATAG TGGATTTGTG 
AAGCAGCTGT TACTTTATTT GACATCTAAA AGTGAAAAGG ATGCTGTGAG CCGAGAGATC 
AGATTAAAGA GATTCCTTCA CGTATTTTTT TCTTCACCAC TTCCTGGAGA AGAGCCCATT 
GGAAGAGTAG AACCAGTGGG TAATGCGCCT TTGTTGGCAT TAGTTCACAA GATGAACAAC 
TGCCTCAGCC AGATGGAACA GTTTCCAGTC AAAGTACATG ACTTCCCCAG TGGGAACGGG 
ACAGGAGGCA GCTTTTCTCT CAACAGAGGA TCACAGGCTT TAAAATTTTT CAACACACAC 
CAGTTAAAAT GCCAGTTACA AAGACATCCA GACTGTGCAA ATGTGAAGCA GTGGAAGGGC 
GGGCCGGTCA AGATTGATCC CCTGGCTTTG GTACAAGCTA TTGAGAGATA CCTTGTAGTT 
AGAGGGTATG GAAGAGTAAG AGAAGATGAT GAAGACAGTG ATGATGATGG CTCTGATGAG 
GAAATAGACG AGTCTCTGGC CGCTCAGTTC TTAAATTCCG GAAACGTAAG GCACAGGCTA 
CAGTTTTATA TTGGAGAACA TTTGCTACCA TATAACATGA CTGTATATCA GGCAGTCCGG 
CAGTTCAGTA TACAAGCTGA GGATGAGCGG GAGTCCACAG ATGATGAGAG CAACCCTCTG 
GGAAGAGCTG GTATTTGGAC AAAGACTCAT ACAATATGGT ACAAACCTGT GAGAGAGGAT 
GAAGAAAGTA ATAAAGATTG TGTTGGTGGT AAAAGAGGAA GAGCCCAAAC GGCTCCAACA 
AAAACATCCC CTAGAAATGC AAAAAAGCAT GATGAGTTAT GGCATGATGG AGTATGCCCA 
TCAGTATCCA ATCCTTTAGA AGTTTACCTC ATCTCCTCGC CACCTGAGAA TATAACTTTT 
GAAGACCCGT CCTTAGATGT GATTCTTCTT CTAAGAGTTT TACATGCCAT CAGCCGATAC 
TGGTATTACT TGTATGATAA TGCAATGTGC AAGGAGATTA TTCCAACTAG TGAATTTATT 
AACAGTAAGT TGACAGCAAA AGCAAATAGG CAGCTTCAAG ATCCTTTAGT AATCATGACA 
GGAAATATCC CAACGTGGCT TACTGAACTA GGAAAAACCT GCCCATTTTT CTTCCCTTTT 
GATACCCGGC AAATGCTTTT TTACGTAACT GCATTTGATC GGGACCGAGC AATGCAAAGA 
TTACTTGATA CCAACCCAGA AATCAACCAG TCGGATTCTC AAGATAGCAG AGTTGCACCT 
AGATTGGATA GAAAAAAACG TACTGTGAAC AGAGAGGAGC TGCTGAAACA AGCGGAGTCT 
GTAATGCAGG ATCTCGGTAG CTCACGGGCC ATGTTAGAAA TCCAGTATGA AAATGAGGTT 
GGTACAGGTC TTGGGCCTAC ACTGGAGTTT TATGCACTTG TATCTCAGGA ACTTCAGAGA 
GCTGACTTGG GTCTCTGGAG AGGTGAAGAA GTGACTCTCA GCAATCCAAA AGGAAGCCAA 
GAAGGGACCA AGTATATTCA AAACCTCCAG GGCCTGTTTG CACTTCCCTT TGGTAGGACA 
GCTAAGCCAG CCCATATCGC AAAGGTTAAA ATGAAGTTTC GCTTCTTAGG AAAATTAATG 
GCCAAGGCTA TCATGGATTT CAGATTGGTG GACCTTCCCC TTGGTTTACC CTTTTATAAG 
TGGATGCTAA GGCAAGAAAC TTCACTGACG TCGCATGATT TGTTTGACAT TGACCCAGTT 
GTAGCCAGAT CAGTGTATCA CCTAGAAGAC ATTGTCAGAC AGAAGAAAAG ACTTGAGCAA 
GATAAATCCC AGACCAAAGA GAGTCTACAA TATGCATTAG AAACCCTGAC TATGAATGGC 
TGCTCAGTTG AAGATCTAGG ATTGGATTTC ACGCTGCCAG GGTTTCCCAA CATTGAACTG 
AAGAAAGGAG GAAAGGATAT ACCAGTTACC ATCCACAATT TAGAGGAGTA TCTAAGACTG 
GTTATATTCT GGGCACTAAA TGAAGGCGTT TCCAGGCAAT TTGATTCCTT CAGAGATGGA 
TTTGAATCTG TCTTCCCACT TAGTCATCTT CAGTACTTCT ACCCGGAAGA ACTGGATCAG 
CTCTTGTGCG GCAGTAAAGC AGACACGTGG GATGCGAAGA CACTGATGGA GTGCTGCAGG 
CCGGACCACG GTTATACTCA TGACAGTCGG GCTGTGAAGT TCTTGTTTGA GATTCTCAGT 
AGTTTTGATA ATGAGCAGCA GAGGTTGTTT CTCCAGTTTG TGACAGGTAG CCCAAGATTG 
CCTGTTGGAG GCTTCCGGAG TCTGAATCCA CCTCTGACAA TTGTCCGGAA GACCTTCGAG 
TCCACGGAGA ACCCCGACGA CTTCTTGCCC TCTGTAATGA CTTGTGTGAA CTACCTTAAG 
TTGCCGGACT ACTCGAGCAT TGACATCATG CGTGAGAAAC TGTTGATTGC AGCGAGAGAA 
GGGCAGCAGT CATTCCATCT TTCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TRIP12 ( XM_005635801.2 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_005635858.1 Canis lupus familiaris thyroid hormone receptor interactor 12 (TRIP12), transcript variant X2, mRNA.

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CloneID	OCe25761
Clone ID Related Accession (Same CDS sequence)	XM_005635800.3 , XM_005635801.2 , XM_005635800.2 , XM_005635801.3 , XM_022409663.1
Accession Version	XM_005635801.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6207bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-09-16
Organism	Canis lupus familiaris(Dog)
Product	E3 ubiquitin-protein ligase TRIP12 isoform X1
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726103.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 17, 2015 this sequence version replaced XM_005635801.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCCAACC GGCCTAATAA CAATCCAGGG GGGTCACTGC GACGTTCACA GAGGAACACT 
GCCGGGGCCC AACCACAAGA CGACTCGATA GGAGGAAGGT CACATTTAGG GCAGGCAAAA 
CATAAGGGAT ATAGCCCTCC TGAGAGTAGA AAATCTAATT CTAAGGCACC CAAAGTGCAG 
TCTAATACTA CTTCTGAACT GTCAAGGGGA CACCTTTCTA AAAGAAGCTG CAGTTCATCA 
TCTGCTGTCA TAGTTCCACA ACCAGAGGAT CCAGACAGAG CCAATACTTC AGAAAGGCAA 
AAAACGGGGC AGGTGCCTAA GAAAGACAAT TCTCGAGGAG TGAAACGCAG TGCTAGTCCA 
GACTACAACA GGACCAATTC TCCTAGCTCT GCCAAAAAAC CAAAAGCACT TCAGCATACT 
GAATCTCCCT CAGAAACAAA TAAGCCACAT AGTAAGTCGA AGAAGAGACA TTTAGACCAG 
GAGCAACAAC TGAAATCTGC ACAGTCGCCA TCAACAAGTA AGGCTCACAC CAGAAAGAGT 
GGGGCCACCG GCGGCTCACG GAGTCAGAAA AGAAAGAGGA CAGAGAGTTC TTGTCTAAAG 
AGTGGTTCTG TGTCTGAGTC AACTGGTGCC GAAGAGAGAT CTGCAAAACC CACCAAGCTG 
GCTTCAAAAT CCGCCGCCTC GGCCAAAGCT GGGTGCAGCA CCATCACTGA TTCTTCCTCC 
GCTGCCTCTA CTTCCTCCTC ATCTTCTGCT GTCGCCTCGG CCTCCTCCAC TGTACCACCA 
GGGGCCAGGG TGAAACAAGG AAAAGACCAG AGCAAGGCCA GGCGTTCCCG TTCAGCATCC 
AGTCCCAGTC CCAGAAGAAG TAGCAGGGAA AAGGAACAGA GTAAAACTGG TGGCTCTTCA 
AAATTCGATT GGGCTGCTCG TTTCAGCCCT AAAGTTAGCC TTCCTAAAAC CAAACTGTCT 
CTTCCAGGGT CTTCTAAGTC AGAGACATCA AAACCTGGAC CTTCTGGATT ACAGGCCAAA 
TTAGCAAGTT TAAGAAAATC TACCAAGAAG CGCAGTGAAT CCCCACCTGC TGAGCTCCCC 
AGTTTGAGGC GGAGCACACG CCAAAAGACC ACGGGCTCCT GTGCTAGTGC CAGTCGGCGA 
GGCTCTGGCC TGGGCAAAAG AGGAGCAGCT GAAGCTCGTC GACAGGAGAA GATGGCGGAC 
CCTGAAAGCA GCCAGGAGAC CGTGAATTCT TCAGCTGCAC GGACAGACGA AACTCCCCAA 
GGAGCTGCAG CCTCTAGTTC TGTTGCAGGG GCTGTGGGCA TGACCACCTC TGGGGAGAGT 
GAATCCGATG ATTCTGAGAT GGGACGACTA CAAGCTTTGT TAGAGGCCAG GGGTCTTCCT 
CCTCATCTGT TTGGCCCTCT CGGTCCTCGG ATGTCACAAC TTTTCCATAG AACAATTGGA 
AGCGGAGCTA GCTCTAAGGC CCAGCAGCTT CTGCAAGGAC TACAAGCAAG TGATGAAAGC 
CAGCAACTCC AGGCAGTTAT TGAAATGTGT CAGTTATTGG TTATGGGAAA TGAGGAGACG 
CTGGGAGGGT TTCCGGTCAA GAGTGTCGTT CCAGCTTTGA TAACATTACT GCAGATGGAG 
CATAATTTCG ATATTATGAA CCATGCTTGT CGAGCCTTAA CATATATGAT GGAAGCACTT 
CCTCGGTCAT CTGCTGTTGT AGTAGATGCA ATTCCTGTCT TTTTAGAAAA GCTGCAAGTT 
ATTCAGTGTA TTGATGTCGC AGAGCAGGCC CTGACTGCCT TGGAGATGTT ATCACGCAGA 
CACAGTAAAG CCATTCTACA GGCGGGTGGT TTGGCAGACT GCTTGCTGTA TCTAGAATTC 
TTCAGCATAA ATGCCCAAAG AAATGCACTA GCAATTGCAG CCAATTGCTG CCAGAGTATC 
ACGCCAGATG AATTTCATTT TGTGGCAGAT TCCCTCCCGT TGCTTACCCA AAGGCTAACC 
CATCAGGACA AAAAGTCAGT TGAAAGCACT TGCCTTTGTT TTGCACGTCT AGTGGACAAC 
TTCCAACATG AGGAGAATTT ACTCCAGCAG GTTGCCTCCA AAGATCTGCT TACGAATGTT 
CAACAACTCT TGGTAGTGAC TCCACCCATT TTAAGTTCTG GGATGTTTAT AATGGTGGTT 
CGCATGTTTT CTCTGATGTG TTCCAACTGT CCAACTTTAG CTGTTCAACT TATGAAGCAA 
AACATTGCAG AAACACTTCA CTTTCTCCTG TGTGGTGCCT CTAATGGAAG TTGTCAGGAA 
CAGATTGATC TTGTTCCACG AAGTCCACAA GAACTGTATG AACTGACATC TCTGATTTGC 
GAACTTATGC CATGTTTACC AAAAGAAGGC ATTTTTGCAG TTGATACCAT GCTGAAGAAG 
GGAAATGCGC AGAACACAGA CGGTGCGATT TGGCAGTGGC GTGATGACCG TGGCCTTTGG 
CATCCCTATA ACAGGATTGA CAGCCGGATC ATTGAGGTAG CAGCCCATCA GGTCGGTGAG 
GATGAGATAA GCTTGTCCAC TCTGGGACGA GTTTATACTA TTGATTTTAA TTCTATGCAG 
CAAATCAATG AGGACACGGG AACAGCACGT GCCATTCAGA GAAAACCTAA CCCCTTAGCC 
AATACTAACA CTAGTGGATA TTCAGAGTTA AAGAAGGATG ATGCTCGAGC ACAGCTTATG 
AAAGAGGATC CGGAACTGGC TAAGTCTTTT ATTAAGACAT TATTTGGTGT TCTTTATGAA 
GTGTATAGTT CCTCAGCAGG ACCTGCGGTC AGACATAAGT GCCTTAGAGC AATTCTTAGG 
ATAATTTATT TTGCGGATGC TGAACTTCTG AAGGATGTCC TGAAAAATCA TGCTGTTTCA 
AGTCACATTG CTTCCATGCT GTCAAGCCAA GACCTGAAGA TAGTAGTTGG AGCACTTCAG 
ATGGCAGAAA TCTTAATGCA GAAGTTACCT GATATTTTTA GTGTTTACTT CAGAAGAGAA 
GGTGTAATGC ATCAAGTAAA ACACTTAGCA GAATCAGAGT CTTTGTTGAC AAGTCCACCA 
AAGGCATGTA CGAATGGGTC AGGATCCCTG GGATCCACGA CGTCAGTCAG CAGTGGAACA 
GCCACGGCCG CCACCAATGC TGCAGCTGAC CTGGGGTCCC CCAGCTTGCA GCACAGCAGA 
GATGACTCTT TAGATCTGAG CCCTCAAGGT CGATTAAGTG ATGTTCTAAA GAGAAAACGG 
CTGCCAAAAC GAGGGCCAAG AAGGCCAAAG TATTCACCTC CAAGAGATGA TGACAAAGTA 
GACAATCAAG CTAAAAGCCC TACCACTACT CAGTCACCTA AATCTTCTTT CCTGGCAAGC 
TTGAATCCAA AAACGTGGGG AAGGTTAAGC GCACAGTCCA ACAGCAACAA CATCGAACCA 
GCACGAACTG CGGGAGTCAG TGGTCTTGCC AGGGCCGCCT CCAAGGACAC CATATCCAAT 
AACAGAGAAA AAATTAAAGG TTGGATTAAG GAACAGGCGC ATAAATTTGT AGAGCGTTAT 
TTCAGTTCTG AGAACATGGA TGGAAGCAAC CCTGCACTGA ATGTCCTTCA GAGACTTTGT 
GCTGCAACTG AACAACTCAA CCTCCAGGTG GATGGTGGAG CTGAGTGCCT TGTAGAAATC 
CGTAGCATAG TCTCAGAGTC TGATGTTTCA TCATTTGAAA TCCAACATAG TGGATTTGTG 
AAGCAGCTGT TACTTTATTT GACATCTAAA AGTGAAAAGG ATGCTGTGAG CCGAGAGATC 
AGATTAAAGA GATTCCTTCA CGTATTTTTT TCTTCACCAC TTCCTGGAGA AGAGCCCATT 
GGAAGAGTAG AACCAGTGGG TAATGCGCCT TTGTTGGCAT TAGTTCACAA GATGAACAAC 
TGCCTCAGCC AGATGGAACA GTTTCCAGTC AAAGTACATG ACTTCCCCAG TGGGAACGGG 
ACAGGAGGCA GCTTTTCTCT CAACAGAGGA TCACAGGCTT TAAAATTTTT CAACACACAC 
CAGTTAAAAT GCCAGTTACA AAGACATCCA GACTGTGCAA ATGTGAAGCA GTGGAAGGGC 
GGGCCGGTCA AGATTGATCC CCTGGCTTTG GTACAAGCTA TTGAGAGATA CCTTGTAGTT 
AGAGGGTATG GAAGAGTAAG AGAAGATGAT GAAGACAGTG ATGATGATGG CTCTGATGAG 
GAAATAGACG AGTCTCTGGC CGCTCAGTTC TTAAATTCCG GAAACGTAAG GCACAGGCTA 
CAGTTTTATA TTGGAGAACA TTTGCTACCA TATAACATGA CTGTATATCA GGCAGTCCGG 
CAGTTCAGTA TACAAGCTGA GGATGAGCGG GAGTCCACAG ATGATGAGAG CAACCCTCTG 
GGAAGAGCTG GTATTTGGAC AAAGACTCAT ACAATATGGT ACAAACCTGT GAGAGAGGAT 
GAAGAAAGTA ATAAAGATTG TGTTGGTGGT AAAAGAGGAA GAGCCCAAAC GGCTCCAACA 
AAAACATCCC CTAGAAATGC AAAAAAGCAT GATGAGTTAT GGCATGATGG AGTATGCCCA 
TCAGTATCCA ATCCTTTAGA AGTTTACCTC ATCTCCTCGC CACCTGAGAA TATAACTTTT 
GAAGACCCGT CCTTAGATGT GATTCTTCTT CTAAGAGTTT TACATGCCAT CAGCCGATAC 
TGGTATTACT TGTATGATAA TGCAATGTGC AAGGAGATTA TTCCAACTAG TGAATTTATT 
AACAGTAAGT TGACAGCAAA AGCAAATAGG CAGCTTCAAG ATCCTTTAGT AATCATGACA 
GGAAATATCC CAACGTGGCT TACTGAACTA GGAAAAACCT GCCCATTTTT CTTCCCTTTT 
GATACCCGGC AAATGCTTTT TTACGTAACT GCATTTGATC GGGACCGAGC AATGCAAAGA 
TTACTTGATA CCAACCCAGA AATCAACCAG TCGGATTCTC AAGATAGCAG AGTTGCACCT 
AGATTGGATA GAAAAAAACG TACTGTGAAC AGAGAGGAGC TGCTGAAACA AGCGGAGTCT 
GTAATGCAGG ATCTCGGTAG CTCACGGGCC ATGTTAGAAA TCCAGTATGA AAATGAGGTT 
GGTACAGGTC TTGGGCCTAC ACTGGAGTTT TATGCACTTG TATCTCAGGA ACTTCAGAGA 
GCTGACTTGG GTCTCTGGAG AGGTGAAGAA GTGACTCTCA GCAATCCAAA AGGAAGCCAA 
GAAGGGACCA AGTATATTCA AAACCTCCAG GGCCTGTTTG CACTTCCCTT TGGTAGGACA 
GCTAAGCCAG CCCATATCGC AAAGGTTAAA ATGAAGTTTC GCTTCTTAGG AAAATTAATG 
GCCAAGGCTA TCATGGATTT CAGATTGGTG GACCTTCCCC TTGGTTTACC CTTTTATAAG 
TGGATGCTAA GGCAAGAAAC TTCACTGACG TCGCATGATT TGTTTGACAT TGACCCAGTT 
GTAGCCAGAT CAGTGTATCA CCTAGAAGAC ATTGTCAGAC AGAAGAAAAG ACTTGAGCAA 
GATAAATCCC AGACCAAAGA GAGTCTACAA TATGCATTAG AAACCCTGAC TATGAATGGC 
TGCTCAGTTG AAGATCTAGG ATTGGATTTC ACGCTGCCAG GGTTTCCCAA CATTGAACTG 
AAGAAAGGAG GAAAGGATAT ACCAGTTACC ATCCACAATT TAGAGGAGTA TCTAAGACTG 
GTTATATTCT GGGCACTAAA TGAAGGCGTT TCCAGGCAAT TTGATTCCTT CAGAGATGGA 
TTTGAATCTG TCTTCCCACT TAGTCATCTT CAGTACTTCT ACCCGGAAGA ACTGGATCAG 
CTCTTGTGCG GCAGTAAAGC AGACACGTGG GATGCGAAGA CACTGATGGA GTGCTGCAGG 
CCGGACCACG GTTATACTCA TGACAGTCGG GCTGTGAAGT TCTTGTTTGA GATTCTCAGT 
AGTTTTGATA ATGAGCAGCA GAGGTTGTTT CTCCAGTTTG TGACAGGTAG CCCAAGATTG 
CCTGTTGGAG GCTTCCGGAG TCTGAATCCA CCTCTGACAA TTGTCCGGAA GACCTTCGAG 
TCCACGGAGA ACCCCGACGA CTTCTTGCCC TCTGTAATGA CTTGTGTGAA CTACCTTAAG 
TTGCCGGACT ACTCGAGCAT TGACATCATG CGTGAGAAAC TGTTGATTGC AGCGAGAGAA 
GGGCAGCAGT CATTCCATCT TTCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005635858.1
CDS	217..6423
Translation	MSNRPNNNPGGSLRRSQRNTAGAQPQDDSIGGRSHLGQAKHKGYSPPESRKSNSKAPKVQSNTTSELSRGHLSKRSCSSSSAVIVPQPEDPDRANTSERQKTGQVPKKDNSRGVKRSASPDYNRTNSPSSAKKPKALQHTESPSETNKPHSKSKKRHLDQEQQLKSAQSPSTSKAHTRKSGATGGSRSQKRKRTESSCLKSGSVSESTGAEERSAKPTKLASKSAASAKAGCSTITDSSSAASTSSSSSAVASASSTVPPGARVKQGKDQSKARRSRSASSPSPRRSSREKEQSKTGGSSKFDWAARFSPKVSLPKTKLSLPGSSKSETSKPGPSGLQAKLASLRKSTKKRSESPPAELPSLRRSTRQKTTGSCASASRRGSGLGKRGAAEARRQEKMADPESSQETVNSSAARTDETPQGAAASSSVAGAVGMTTSGESESDDSEMGRLQALLEARGLPPHLFGPLGPRMSQLFHRTIGSGASSKAQQLLQGLQASDESQQLQAVIEMCQLLVMGNEETLGGFPVKSVVPALITLLQMEHNFDIMNHACRALTYMMEALPRSSAVVVDAIPVFLEKLQVIQCIDVAEQALTALEMLSRRHSKAILQAGGLADCLLYLEFFSINAQRNALAIAANCCQSITPDEFHFVADSLPLLTQRLTHQDKKSVESTCLCFARLVDNFQHEENLLQQVASKDLLTNVQQLLVVTPPILSSGMFIMVVRMFSLMCSNCPTLAVQLMKQNIAETLHFLLCGASNGSCQEQIDLVPRSPQELYELTSLICELMPCLPKEGIFAVDTMLKKGNAQNTDGAIWQWRDDRGLWHPYNRIDSRIIEVAAHQVGEDEISLSTLGRVYTIDFNSMQQINEDTGTARAIQRKPNPLANTNTSGYSELKKDDARAQLMKEDPELAKSFIKTLFGVLYEVYSSSAGPAVRHKCLRAILRIIYFADAELLKDVLKNHAVSSHIASMLSSQDLKIVVGALQMAEILMQKLPDIFSVYFRREGVMHQVKHLAESESLLTSPPKACTNGSGSLGSTTSVSSGTATAATNAAADLGSPSLQHSRDDSLDLSPQGRLSDVLKRKRLPKRGPRRPKYSPPRDDDKVDNQAKSPTTTQSPKSSFLASLNPKTWGRLSAQSNSNNIEPARTAGVSGLARAASKDTISNNREKIKGWIKEQAHKFVERYFSSENMDGSNPALNVLQRLCAATEQLNLQVDGGAECLVEIRSIVSESDVSSFEIQHSGFVKQLLLYLTSKSEKDAVSREIRLKRFLHVFFSSPLPGEEPIGRVEPVGNAPLLALVHKMNNCLSQMEQFPVKVHDFPSGNGTGGSFSLNRGSQALKFFNTHQLKCQLQRHPDCANVKQWKGGPVKIDPLALVQAIERYLVVRGYGRVREDDEDSDDDGSDEEIDESLAAQFLNSGNVRHRLQFYIGEHLLPYNMTVYQAVRQFSIQAEDERESTDDESNPLGRAGIWTKTHTIWYKPVREDEESNKDCVGGKRGRAQTAPTKTSPRNAKKHDELWHDGVCPSVSNPLEVYLISSPPENITFEDPSLDVILLLRVLHAISRYWYYLYDNAMCKEIIPTSEFINSKLTAKANRQLQDPLVIMTGNIPTWLTELGKTCPFFFPFDTRQMLFYVTAFDRDRAMQRLLDTNPEINQSDSQDSRVAPRLDRKKRTVNREELLKQAESVMQDLGSSRAMLEIQYENEVGTGLGPTLEFYALVSQELQRADLGLWRGEEVTLSNPKGSQEGTKYIQNLQGLFALPFGRTAKPAHIAKVKMKFRFLGKLMAKAIMDFRLVDLPLGLPFYKWMLRQETSLTSHDLFDIDPVVARSVYHLEDIVRQKKRLEQDKSQTKESLQYALETLTMNGCSVEDLGLDFTLPGFPNIELKKGGKDIPVTIHNLEEYLRLVIFWALNEGVSRQFDSFRDGFESVFPLSHLQYFYPEELDQLLCGSKADTWDAKTLMECCRPDHGYTHDSRAVKFLFEILSSFDNEQQRLFLQFVTGSPRLPVGGFRSLNPPLTIVRKTFESTENPDDFLPSVMTCVNYLKLPDYSSIDIMREKLLIAAREGQQSFHLS

Target ORF information:

RefSeq Version	XM_005635801.2
Organism	Canis lupus familiaris(Dog)
Definition	Canis lupus familiaris thyroid hormone receptor interactor 12 (TRIP12), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005635801.2

ORF Insert Sequence:

ATGTCCAACC GGCCTAATAA CAATCCAGGG GGGTCACTGC GACGTTCACA GAGGAACACT 
GCCGGGGCCC AACCACAAGA CGACTCGATA GGAGGAAGGT CACATTTAGG GCAGGCAAAA 
CATAAGGGAT ATAGCCCTCC TGAGAGTAGA AAATCTAATT CTAAGGCACC CAAAGTGCAG 
TCTAATACTA CTTCTGAACT GTCAAGGGGA CACCTTTCTA AAAGAAGCTG CAGTTCATCA 
TCTGCTGTCA TAGTTCCACA ACCAGAGGAT CCAGACAGAG CCAATACTTC AGAAAGGCAA 
AAAACGGGGC AGGTGCCTAA GAAAGACAAT TCTCGAGGAG TGAAACGCAG TGCTAGTCCA 
GACTACAACA GGACCAATTC TCCTAGCTCT GCCAAAAAAC CAAAAGCACT TCAGCATACT 
GAATCTCCCT CAGAAACAAA TAAGCCACAT AGTAAGTCGA AGAAGAGACA TTTAGACCAG 
GAGCAACAAC TGAAATCTGC ACAGTCGCCA TCAACAAGTA AGGCTCACAC CAGAAAGAGT 
GGGGCCACCG GCGGCTCACG GAGTCAGAAA AGAAAGAGGA CAGAGAGTTC TTGTCTAAAG 
AGTGGTTCTG TGTCTGAGTC AACTGGTGCC GAAGAGAGAT CTGCAAAACC CACCAAGCTG 
GCTTCAAAAT CCGCCGCCTC GGCCAAAGCT GGGTGCAGCA CCATCACTGA TTCTTCCTCC 
GCTGCCTCTA CTTCCTCCTC ATCTTCTGCT GTCGCCTCGG CCTCCTCCAC TGTACCACCA 
GGGGCCAGGG TGAAACAAGG AAAAGACCAG AGCAAGGCCA GGCGTTCCCG TTCAGCATCC 
AGTCCCAGTC CCAGAAGAAG TAGCAGGGAA AAGGAACAGA GTAAAACTGG TGGCTCTTCA 
AAATTCGATT GGGCTGCTCG TTTCAGCCCT AAAGTTAGCC TTCCTAAAAC CAAACTGTCT 
CTTCCAGGGT CTTCTAAGTC AGAGACATCA AAACCTGGAC CTTCTGGATT ACAGGCCAAA 
TTAGCAAGTT TAAGAAAATC TACCAAGAAG CGCAGTGAAT CCCCACCTGC TGAGCTCCCC 
AGTTTGAGGC GGAGCACACG CCAAAAGACC ACGGGCTCCT GTGCTAGTGC CAGTCGGCGA 
GGCTCTGGCC TGGGCAAAAG AGGAGCAGCT GAAGCTCGTC GACAGGAGAA GATGGCGGAC 
CCTGAAAGCA GCCAGGAGAC CGTGAATTCT TCAGCTGCAC GGACAGACGA AACTCCCCAA 
GGAGCTGCAG CCTCTAGTTC TGTTGCAGGG GCTGTGGGCA TGACCACCTC TGGGGAGAGT 
GAATCCGATG ATTCTGAGAT GGGACGACTA CAAGCTTTGT TAGAGGCCAG GGGTCTTCCT 
CCTCATCTGT TTGGCCCTCT CGGTCCTCGG ATGTCACAAC TTTTCCATAG AACAATTGGA 
AGCGGAGCTA GCTCTAAGGC CCAGCAGCTT CTGCAAGGAC TACAAGCAAG TGATGAAAGC 
CAGCAACTCC AGGCAGTTAT TGAAATGTGT CAGTTATTGG TTATGGGAAA TGAGGAGACG 
CTGGGAGGGT TTCCGGTCAA GAGTGTCGTT CCAGCTTTGA TAACATTACT GCAGATGGAG 
CATAATTTCG ATATTATGAA CCATGCTTGT CGAGCCTTAA CATATATGAT GGAAGCACTT 
CCTCGGTCAT CTGCTGTTGT AGTAGATGCA ATTCCTGTCT TTTTAGAAAA GCTGCAAGTT 
ATTCAGTGTA TTGATGTCGC AGAGCAGGCC CTGACTGCCT TGGAGATGTT ATCACGCAGA 
CACAGTAAAG CCATTCTACA GGCGGGTGGT TTGGCAGACT GCTTGCTGTA TCTAGAATTC 
TTCAGCATAA ATGCCCAAAG AAATGCACTA GCAATTGCAG CCAATTGCTG CCAGAGTATC 
ACGCCAGATG AATTTCATTT TGTGGCAGAT TCCCTCCCGT TGCTTACCCA AAGGCTAACC 
CATCAGGACA AAAAGTCAGT TGAAAGCACT TGCCTTTGTT TTGCACGTCT AGTGGACAAC 
TTCCAACATG AGGAGAATTT ACTCCAGCAG GTTGCCTCCA AAGATCTGCT TACGAATGTT 
CAACAACTCT TGGTAGTGAC TCCACCCATT TTAAGTTCTG GGATGTTTAT AATGGTGGTT 
CGCATGTTTT CTCTGATGTG TTCCAACTGT CCAACTTTAG CTGTTCAACT TATGAAGCAA 
AACATTGCAG AAACACTTCA CTTTCTCCTG TGTGGTGCCT CTAATGGAAG TTGTCAGGAA 
CAGATTGATC TTGTTCCACG AAGTCCACAA GAACTGTATG AACTGACATC TCTGATTTGC 
GAACTTATGC CATGTTTACC AAAAGAAGGC ATTTTTGCAG TTGATACCAT GCTGAAGAAG 
GGAAATGCGC AGAACACAGA CGGTGCGATT TGGCAGTGGC GTGATGACCG TGGCCTTTGG 
CATCCCTATA ACAGGATTGA CAGCCGGATC ATTGAGGTAG CAGCCCATCA GGTCGGTGAG 
GATGAGATAA GCTTGTCCAC TCTGGGACGA GTTTATACTA TTGATTTTAA TTCTATGCAG 
CAAATCAATG AGGACACGGG AACAGCACGT GCCATTCAGA GAAAACCTAA CCCCTTAGCC 
AATACTAACA CTAGTGGATA TTCAGAGTTA AAGAAGGATG ATGCTCGAGC ACAGCTTATG 
AAAGAGGATC CGGAACTGGC TAAGTCTTTT ATTAAGACAT TATTTGGTGT TCTTTATGAA 
GTGTATAGTT CCTCAGCAGG ACCTGCGGTC AGACATAAGT GCCTTAGAGC AATTCTTAGG 
ATAATTTATT TTGCGGATGC TGAACTTCTG AAGGATGTCC TGAAAAATCA TGCTGTTTCA 
AGTCACATTG CTTCCATGCT GTCAAGCCAA GACCTGAAGA TAGTAGTTGG AGCACTTCAG 
ATGGCAGAAA TCTTAATGCA GAAGTTACCT GATATTTTTA GTGTTTACTT CAGAAGAGAA 
GGTGTAATGC ATCAAGTAAA ACACTTAGCA GAATCAGAGT CTTTGTTGAC AAGTCCACCA 
AAGGCATGTA CGAATGGGTC AGGATCCCTG GGATCCACGA CGTCAGTCAG CAGTGGAACA 
GCCACGGCCG CCACCAATGC TGCAGCTGAC CTGGGGTCCC CCAGCTTGCA GCACAGCAGA 
GATGACTCTT TAGATCTGAG CCCTCAAGGT CGATTAAGTG ATGTTCTAAA GAGAAAACGG 
CTGCCAAAAC GAGGGCCAAG AAGGCCAAAG TATTCACCTC CAAGAGATGA TGACAAAGTA 
GACAATCAAG CTAAAAGCCC TACCACTACT CAGTCACCTA AATCTTCTTT CCTGGCAAGC 
TTGAATCCAA AAACGTGGGG AAGGTTAAGC GCACAGTCCA ACAGCAACAA CATCGAACCA 
GCACGAACTG CGGGAGTCAG TGGTCTTGCC AGGGCCGCCT CCAAGGACAC CATATCCAAT 
AACAGAGAAA AAATTAAAGG TTGGATTAAG GAACAGGCGC ATAAATTTGT AGAGCGTTAT 
TTCAGTTCTG AGAACATGGA TGGAAGCAAC CCTGCACTGA ATGTCCTTCA GAGACTTTGT 
GCTGCAACTG AACAACTCAA CCTCCAGGTG GATGGTGGAG CTGAGTGCCT TGTAGAAATC 
CGTAGCATAG TCTCAGAGTC TGATGTTTCA TCATTTGAAA TCCAACATAG TGGATTTGTG 
AAGCAGCTGT TACTTTATTT GACATCTAAA AGTGAAAAGG ATGCTGTGAG CCGAGAGATC 
AGATTAAAGA GATTCCTTCA CGTATTTTTT TCTTCACCAC TTCCTGGAGA AGAGCCCATT 
GGAAGAGTAG AACCAGTGGG TAATGCGCCT TTGTTGGCAT TAGTTCACAA GATGAACAAC 
TGCCTCAGCC AGATGGAACA GTTTCCAGTC AAAGTACATG ACTTCCCCAG TGGGAACGGG 
ACAGGAGGCA GCTTTTCTCT CAACAGAGGA TCACAGGCTT TAAAATTTTT CAACACACAC 
CAGTTAAAAT GCCAGTTACA AAGACATCCA GACTGTGCAA ATGTGAAGCA GTGGAAGGGC 
GGGCCGGTCA AGATTGATCC CCTGGCTTTG GTACAAGCTA TTGAGAGATA CCTTGTAGTT 
AGAGGGTATG GAAGAGTAAG AGAAGATGAT GAAGACAGTG ATGATGATGG CTCTGATGAG 
GAAATAGACG AGTCTCTGGC CGCTCAGTTC TTAAATTCCG GAAACGTAAG GCACAGGCTA 
CAGTTTTATA TTGGAGAACA TTTGCTACCA TATAACATGA CTGTATATCA GGCAGTCCGG 
CAGTTCAGTA TACAAGCTGA GGATGAGCGG GAGTCCACAG ATGATGAGAG CAACCCTCTG 
GGAAGAGCTG GTATTTGGAC AAAGACTCAT ACAATATGGT ACAAACCTGT GAGAGAGGAT 
GAAGAAAGTA ATAAAGATTG TGTTGGTGGT AAAAGAGGAA GAGCCCAAAC GGCTCCAACA 
AAAACATCCC CTAGAAATGC AAAAAAGCAT GATGAGTTAT GGCATGATGG AGTATGCCCA 
TCAGTATCCA ATCCTTTAGA AGTTTACCTC ATCTCCTCGC CACCTGAGAA TATAACTTTT 
GAAGACCCGT CCTTAGATGT GATTCTTCTT CTAAGAGTTT TACATGCCAT CAGCCGATAC 
TGGTATTACT TGTATGATAA TGCAATGTGC AAGGAGATTA TTCCAACTAG TGAATTTATT 
AACAGTAAGT TGACAGCAAA AGCAAATAGG CAGCTTCAAG ATCCTTTAGT AATCATGACA 
GGAAATATCC CAACGTGGCT TACTGAACTA GGAAAAACCT GCCCATTTTT CTTCCCTTTT 
GATACCCGGC AAATGCTTTT TTACGTAACT GCATTTGATC GGGACCGAGC AATGCAAAGA 
TTACTTGATA CCAACCCAGA AATCAACCAG TCGGATTCTC AAGATAGCAG AGTTGCACCT 
AGATTGGATA GAAAAAAACG TACTGTGAAC AGAGAGGAGC TGCTGAAACA AGCGGAGTCT 
GTAATGCAGG ATCTCGGTAG CTCACGGGCC ATGTTAGAAA TCCAGTATGA AAATGAGGTT 
GGTACAGGTC TTGGGCCTAC ACTGGAGTTT TATGCACTTG TATCTCAGGA ACTTCAGAGA 
GCTGACTTGG GTCTCTGGAG AGGTGAAGAA GTGACTCTCA GCAATCCAAA AGGAAGCCAA 
GAAGGGACCA AGTATATTCA AAACCTCCAG GGCCTGTTTG CACTTCCCTT TGGTAGGACA 
GCTAAGCCAG CCCATATCGC AAAGGTTAAA ATGAAGTTTC GCTTCTTAGG AAAATTAATG 
GCCAAGGCTA TCATGGATTT CAGATTGGTG GACCTTCCCC TTGGTTTACC CTTTTATAAG 
TGGATGCTAA GGCAAGAAAC TTCACTGACG TCGCATGATT TGTTTGACAT TGACCCAGTT 
GTAGCCAGAT CAGTGTATCA CCTAGAAGAC ATTGTCAGAC AGAAGAAAAG ACTTGAGCAA 
GATAAATCCC AGACCAAAGA GAGTCTACAA TATGCATTAG AAACCCTGAC TATGAATGGC 
TGCTCAGTTG AAGATCTAGG ATTGGATTTC ACGCTGCCAG GGTTTCCCAA CATTGAACTG 
AAGAAAGGAG GAAAGGATAT ACCAGTTACC ATCCACAATT TAGAGGAGTA TCTAAGACTG 
GTTATATTCT GGGCACTAAA TGAAGGCGTT TCCAGGCAAT TTGATTCCTT CAGAGATGGA 
TTTGAATCTG TCTTCCCACT TAGTCATCTT CAGTACTTCT ACCCGGAAGA ACTGGATCAG 
CTCTTGTGCG GCAGTAAAGC AGACACGTGG GATGCGAAGA CACTGATGGA GTGCTGCAGG 
CCGGACCACG GTTATACTCA TGACAGTCGG GCTGTGAAGT TCTTGTTTGA GATTCTCAGT 
AGTTTTGATA ATGAGCAGCA GAGGTTGTTT CTCCAGTTTG TGACAGGTAG CCCAAGATTG 
CCTGTTGGAG GCTTCCGGAG TCTGAATCCA CCTCTGACAA TTGTCCGGAA GACCTTCGAG 
TCCACGGAGA ACCCCGACGA CTTCTTGCCC TCTGTAATGA CTTGTGTGAA CTACCTTAAG 
TTGCCGGACT ACTCGAGCAT TGACATCATG CGTGAGAAAC TGTTGATTGC AGCGAGAGAA 
GGGCAGCAGT CATTCCATCT TTCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TRIP12 ( XM_005635800.2 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_005635857.1 Canis lupus familiaris thyroid hormone receptor interactor 12 (TRIP12), transcript variant X1, mRNA.

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CloneID	OCe25761
Clone ID Related Accession (Same CDS sequence)	XM_005635800.3 , XM_005635801.2 , XM_005635800.2 , XM_005635801.3 , XM_022409663.1
Accession Version	XM_005635800.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6207bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-09-16
Organism	Canis lupus familiaris(Dog)
Product	E3 ubiquitin-protein ligase TRIP12 isoform X1
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726103.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 17, 2015 this sequence version replaced XM_005635800.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCCAACC GGCCTAATAA CAATCCAGGG GGGTCACTGC GACGTTCACA GAGGAACACT 
GCCGGGGCCC AACCACAAGA CGACTCGATA GGAGGAAGGT CACATTTAGG GCAGGCAAAA 
CATAAGGGAT ATAGCCCTCC TGAGAGTAGA AAATCTAATT CTAAGGCACC CAAAGTGCAG 
TCTAATACTA CTTCTGAACT GTCAAGGGGA CACCTTTCTA AAAGAAGCTG CAGTTCATCA 
TCTGCTGTCA TAGTTCCACA ACCAGAGGAT CCAGACAGAG CCAATACTTC AGAAAGGCAA 
AAAACGGGGC AGGTGCCTAA GAAAGACAAT TCTCGAGGAG TGAAACGCAG TGCTAGTCCA 
GACTACAACA GGACCAATTC TCCTAGCTCT GCCAAAAAAC CAAAAGCACT TCAGCATACT 
GAATCTCCCT CAGAAACAAA TAAGCCACAT AGTAAGTCGA AGAAGAGACA TTTAGACCAG 
GAGCAACAAC TGAAATCTGC ACAGTCGCCA TCAACAAGTA AGGCTCACAC CAGAAAGAGT 
GGGGCCACCG GCGGCTCACG GAGTCAGAAA AGAAAGAGGA CAGAGAGTTC TTGTCTAAAG 
AGTGGTTCTG TGTCTGAGTC AACTGGTGCC GAAGAGAGAT CTGCAAAACC CACCAAGCTG 
GCTTCAAAAT CCGCCGCCTC GGCCAAAGCT GGGTGCAGCA CCATCACTGA TTCTTCCTCC 
GCTGCCTCTA CTTCCTCCTC ATCTTCTGCT GTCGCCTCGG CCTCCTCCAC TGTACCACCA 
GGGGCCAGGG TGAAACAAGG AAAAGACCAG AGCAAGGCCA GGCGTTCCCG TTCAGCATCC 
AGTCCCAGTC CCAGAAGAAG TAGCAGGGAA AAGGAACAGA GTAAAACTGG TGGCTCTTCA 
AAATTCGATT GGGCTGCTCG TTTCAGCCCT AAAGTTAGCC TTCCTAAAAC CAAACTGTCT 
CTTCCAGGGT CTTCTAAGTC AGAGACATCA AAACCTGGAC CTTCTGGATT ACAGGCCAAA 
TTAGCAAGTT TAAGAAAATC TACCAAGAAG CGCAGTGAAT CCCCACCTGC TGAGCTCCCC 
AGTTTGAGGC GGAGCACACG CCAAAAGACC ACGGGCTCCT GTGCTAGTGC CAGTCGGCGA 
GGCTCTGGCC TGGGCAAAAG AGGAGCAGCT GAAGCTCGTC GACAGGAGAA GATGGCGGAC 
CCTGAAAGCA GCCAGGAGAC CGTGAATTCT TCAGCTGCAC GGACAGACGA AACTCCCCAA 
GGAGCTGCAG CCTCTAGTTC TGTTGCAGGG GCTGTGGGCA TGACCACCTC TGGGGAGAGT 
GAATCCGATG ATTCTGAGAT GGGACGACTA CAAGCTTTGT TAGAGGCCAG GGGTCTTCCT 
CCTCATCTGT TTGGCCCTCT CGGTCCTCGG ATGTCACAAC TTTTCCATAG AACAATTGGA 
AGCGGAGCTA GCTCTAAGGC CCAGCAGCTT CTGCAAGGAC TACAAGCAAG TGATGAAAGC 
CAGCAACTCC AGGCAGTTAT TGAAATGTGT CAGTTATTGG TTATGGGAAA TGAGGAGACG 
CTGGGAGGGT TTCCGGTCAA GAGTGTCGTT CCAGCTTTGA TAACATTACT GCAGATGGAG 
CATAATTTCG ATATTATGAA CCATGCTTGT CGAGCCTTAA CATATATGAT GGAAGCACTT 
CCTCGGTCAT CTGCTGTTGT AGTAGATGCA ATTCCTGTCT TTTTAGAAAA GCTGCAAGTT 
ATTCAGTGTA TTGATGTCGC AGAGCAGGCC CTGACTGCCT TGGAGATGTT ATCACGCAGA 
CACAGTAAAG CCATTCTACA GGCGGGTGGT TTGGCAGACT GCTTGCTGTA TCTAGAATTC 
TTCAGCATAA ATGCCCAAAG AAATGCACTA GCAATTGCAG CCAATTGCTG CCAGAGTATC 
ACGCCAGATG AATTTCATTT TGTGGCAGAT TCCCTCCCGT TGCTTACCCA AAGGCTAACC 
CATCAGGACA AAAAGTCAGT TGAAAGCACT TGCCTTTGTT TTGCACGTCT AGTGGACAAC 
TTCCAACATG AGGAGAATTT ACTCCAGCAG GTTGCCTCCA AAGATCTGCT TACGAATGTT 
CAACAACTCT TGGTAGTGAC TCCACCCATT TTAAGTTCTG GGATGTTTAT AATGGTGGTT 
CGCATGTTTT CTCTGATGTG TTCCAACTGT CCAACTTTAG CTGTTCAACT TATGAAGCAA 
AACATTGCAG AAACACTTCA CTTTCTCCTG TGTGGTGCCT CTAATGGAAG TTGTCAGGAA 
CAGATTGATC TTGTTCCACG AAGTCCACAA GAACTGTATG AACTGACATC TCTGATTTGC 
GAACTTATGC CATGTTTACC AAAAGAAGGC ATTTTTGCAG TTGATACCAT GCTGAAGAAG 
GGAAATGCGC AGAACACAGA CGGTGCGATT TGGCAGTGGC GTGATGACCG TGGCCTTTGG 
CATCCCTATA ACAGGATTGA CAGCCGGATC ATTGAGGTAG CAGCCCATCA GGTCGGTGAG 
GATGAGATAA GCTTGTCCAC TCTGGGACGA GTTTATACTA TTGATTTTAA TTCTATGCAG 
CAAATCAATG AGGACACGGG AACAGCACGT GCCATTCAGA GAAAACCTAA CCCCTTAGCC 
AATACTAACA CTAGTGGATA TTCAGAGTTA AAGAAGGATG ATGCTCGAGC ACAGCTTATG 
AAAGAGGATC CGGAACTGGC TAAGTCTTTT ATTAAGACAT TATTTGGTGT TCTTTATGAA 
GTGTATAGTT CCTCAGCAGG ACCTGCGGTC AGACATAAGT GCCTTAGAGC AATTCTTAGG 
ATAATTTATT TTGCGGATGC TGAACTTCTG AAGGATGTCC TGAAAAATCA TGCTGTTTCA 
AGTCACATTG CTTCCATGCT GTCAAGCCAA GACCTGAAGA TAGTAGTTGG AGCACTTCAG 
ATGGCAGAAA TCTTAATGCA GAAGTTACCT GATATTTTTA GTGTTTACTT CAGAAGAGAA 
GGTGTAATGC ATCAAGTAAA ACACTTAGCA GAATCAGAGT CTTTGTTGAC AAGTCCACCA 
AAGGCATGTA CGAATGGGTC AGGATCCCTG GGATCCACGA CGTCAGTCAG CAGTGGAACA 
GCCACGGCCG CCACCAATGC TGCAGCTGAC CTGGGGTCCC CCAGCTTGCA GCACAGCAGA 
GATGACTCTT TAGATCTGAG CCCTCAAGGT CGATTAAGTG ATGTTCTAAA GAGAAAACGG 
CTGCCAAAAC GAGGGCCAAG AAGGCCAAAG TATTCACCTC CAAGAGATGA TGACAAAGTA 
GACAATCAAG CTAAAAGCCC TACCACTACT CAGTCACCTA AATCTTCTTT CCTGGCAAGC 
TTGAATCCAA AAACGTGGGG AAGGTTAAGC GCACAGTCCA ACAGCAACAA CATCGAACCA 
GCACGAACTG CGGGAGTCAG TGGTCTTGCC AGGGCCGCCT CCAAGGACAC CATATCCAAT 
AACAGAGAAA AAATTAAAGG TTGGATTAAG GAACAGGCGC ATAAATTTGT AGAGCGTTAT 
TTCAGTTCTG AGAACATGGA TGGAAGCAAC CCTGCACTGA ATGTCCTTCA GAGACTTTGT 
GCTGCAACTG AACAACTCAA CCTCCAGGTG GATGGTGGAG CTGAGTGCCT TGTAGAAATC 
CGTAGCATAG TCTCAGAGTC TGATGTTTCA TCATTTGAAA TCCAACATAG TGGATTTGTG 
AAGCAGCTGT TACTTTATTT GACATCTAAA AGTGAAAAGG ATGCTGTGAG CCGAGAGATC 
AGATTAAAGA GATTCCTTCA CGTATTTTTT TCTTCACCAC TTCCTGGAGA AGAGCCCATT 
GGAAGAGTAG AACCAGTGGG TAATGCGCCT TTGTTGGCAT TAGTTCACAA GATGAACAAC 
TGCCTCAGCC AGATGGAACA GTTTCCAGTC AAAGTACATG ACTTCCCCAG TGGGAACGGG 
ACAGGAGGCA GCTTTTCTCT CAACAGAGGA TCACAGGCTT TAAAATTTTT CAACACACAC 
CAGTTAAAAT GCCAGTTACA AAGACATCCA GACTGTGCAA ATGTGAAGCA GTGGAAGGGC 
GGGCCGGTCA AGATTGATCC CCTGGCTTTG GTACAAGCTA TTGAGAGATA CCTTGTAGTT 
AGAGGGTATG GAAGAGTAAG AGAAGATGAT GAAGACAGTG ATGATGATGG CTCTGATGAG 
GAAATAGACG AGTCTCTGGC CGCTCAGTTC TTAAATTCCG GAAACGTAAG GCACAGGCTA 
CAGTTTTATA TTGGAGAACA TTTGCTACCA TATAACATGA CTGTATATCA GGCAGTCCGG 
CAGTTCAGTA TACAAGCTGA GGATGAGCGG GAGTCCACAG ATGATGAGAG CAACCCTCTG 
GGAAGAGCTG GTATTTGGAC AAAGACTCAT ACAATATGGT ACAAACCTGT GAGAGAGGAT 
GAAGAAAGTA ATAAAGATTG TGTTGGTGGT AAAAGAGGAA GAGCCCAAAC GGCTCCAACA 
AAAACATCCC CTAGAAATGC AAAAAAGCAT GATGAGTTAT GGCATGATGG AGTATGCCCA 
TCAGTATCCA ATCCTTTAGA AGTTTACCTC ATCTCCTCGC CACCTGAGAA TATAACTTTT 
GAAGACCCGT CCTTAGATGT GATTCTTCTT CTAAGAGTTT TACATGCCAT CAGCCGATAC 
TGGTATTACT TGTATGATAA TGCAATGTGC AAGGAGATTA TTCCAACTAG TGAATTTATT 
AACAGTAAGT TGACAGCAAA AGCAAATAGG CAGCTTCAAG ATCCTTTAGT AATCATGACA 
GGAAATATCC CAACGTGGCT TACTGAACTA GGAAAAACCT GCCCATTTTT CTTCCCTTTT 
GATACCCGGC AAATGCTTTT TTACGTAACT GCATTTGATC GGGACCGAGC AATGCAAAGA 
TTACTTGATA CCAACCCAGA AATCAACCAG TCGGATTCTC AAGATAGCAG AGTTGCACCT 
AGATTGGATA GAAAAAAACG TACTGTGAAC AGAGAGGAGC TGCTGAAACA AGCGGAGTCT 
GTAATGCAGG ATCTCGGTAG CTCACGGGCC ATGTTAGAAA TCCAGTATGA AAATGAGGTT 
GGTACAGGTC TTGGGCCTAC ACTGGAGTTT TATGCACTTG TATCTCAGGA ACTTCAGAGA 
GCTGACTTGG GTCTCTGGAG AGGTGAAGAA GTGACTCTCA GCAATCCAAA AGGAAGCCAA 
GAAGGGACCA AGTATATTCA AAACCTCCAG GGCCTGTTTG CACTTCCCTT TGGTAGGACA 
GCTAAGCCAG CCCATATCGC AAAGGTTAAA ATGAAGTTTC GCTTCTTAGG AAAATTAATG 
GCCAAGGCTA TCATGGATTT CAGATTGGTG GACCTTCCCC TTGGTTTACC CTTTTATAAG 
TGGATGCTAA GGCAAGAAAC TTCACTGACG TCGCATGATT TGTTTGACAT TGACCCAGTT 
GTAGCCAGAT CAGTGTATCA CCTAGAAGAC ATTGTCAGAC AGAAGAAAAG ACTTGAGCAA 
GATAAATCCC AGACCAAAGA GAGTCTACAA TATGCATTAG AAACCCTGAC TATGAATGGC 
TGCTCAGTTG AAGATCTAGG ATTGGATTTC ACGCTGCCAG GGTTTCCCAA CATTGAACTG 
AAGAAAGGAG GAAAGGATAT ACCAGTTACC ATCCACAATT TAGAGGAGTA TCTAAGACTG 
GTTATATTCT GGGCACTAAA TGAAGGCGTT TCCAGGCAAT TTGATTCCTT CAGAGATGGA 
TTTGAATCTG TCTTCCCACT TAGTCATCTT CAGTACTTCT ACCCGGAAGA ACTGGATCAG 
CTCTTGTGCG GCAGTAAAGC AGACACGTGG GATGCGAAGA CACTGATGGA GTGCTGCAGG 
CCGGACCACG GTTATACTCA TGACAGTCGG GCTGTGAAGT TCTTGTTTGA GATTCTCAGT 
AGTTTTGATA ATGAGCAGCA GAGGTTGTTT CTCCAGTTTG TGACAGGTAG CCCAAGATTG 
CCTGTTGGAG GCTTCCGGAG TCTGAATCCA CCTCTGACAA TTGTCCGGAA GACCTTCGAG 
TCCACGGAGA ACCCCGACGA CTTCTTGCCC TCTGTAATGA CTTGTGTGAA CTACCTTAAG 
TTGCCGGACT ACTCGAGCAT TGACATCATG CGTGAGAAAC TGTTGATTGC AGCGAGAGAA 
GGGCAGCAGT CATTCCATCT TTCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005635857.1
CDS	188..6394
Translation	MSNRPNNNPGGSLRRSQRNTAGAQPQDDSIGGRSHLGQAKHKGYSPPESRKSNSKAPKVQSNTTSELSRGHLSKRSCSSSSAVIVPQPEDPDRANTSERQKTGQVPKKDNSRGVKRSASPDYNRTNSPSSAKKPKALQHTESPSETNKPHSKSKKRHLDQEQQLKSAQSPSTSKAHTRKSGATGGSRSQKRKRTESSCLKSGSVSESTGAEERSAKPTKLASKSAASAKAGCSTITDSSSAASTSSSSSAVASASSTVPPGARVKQGKDQSKARRSRSASSPSPRRSSREKEQSKTGGSSKFDWAARFSPKVSLPKTKLSLPGSSKSETSKPGPSGLQAKLASLRKSTKKRSESPPAELPSLRRSTRQKTTGSCASASRRGSGLGKRGAAEARRQEKMADPESSQETVNSSAARTDETPQGAAASSSVAGAVGMTTSGESESDDSEMGRLQALLEARGLPPHLFGPLGPRMSQLFHRTIGSGASSKAQQLLQGLQASDESQQLQAVIEMCQLLVMGNEETLGGFPVKSVVPALITLLQMEHNFDIMNHACRALTYMMEALPRSSAVVVDAIPVFLEKLQVIQCIDVAEQALTALEMLSRRHSKAILQAGGLADCLLYLEFFSINAQRNALAIAANCCQSITPDEFHFVADSLPLLTQRLTHQDKKSVESTCLCFARLVDNFQHEENLLQQVASKDLLTNVQQLLVVTPPILSSGMFIMVVRMFSLMCSNCPTLAVQLMKQNIAETLHFLLCGASNGSCQEQIDLVPRSPQELYELTSLICELMPCLPKEGIFAVDTMLKKGNAQNTDGAIWQWRDDRGLWHPYNRIDSRIIEVAAHQVGEDEISLSTLGRVYTIDFNSMQQINEDTGTARAIQRKPNPLANTNTSGYSELKKDDARAQLMKEDPELAKSFIKTLFGVLYEVYSSSAGPAVRHKCLRAILRIIYFADAELLKDVLKNHAVSSHIASMLSSQDLKIVVGALQMAEILMQKLPDIFSVYFRREGVMHQVKHLAESESLLTSPPKACTNGSGSLGSTTSVSSGTATAATNAAADLGSPSLQHSRDDSLDLSPQGRLSDVLKRKRLPKRGPRRPKYSPPRDDDKVDNQAKSPTTTQSPKSSFLASLNPKTWGRLSAQSNSNNIEPARTAGVSGLARAASKDTISNNREKIKGWIKEQAHKFVERYFSSENMDGSNPALNVLQRLCAATEQLNLQVDGGAECLVEIRSIVSESDVSSFEIQHSGFVKQLLLYLTSKSEKDAVSREIRLKRFLHVFFSSPLPGEEPIGRVEPVGNAPLLALVHKMNNCLSQMEQFPVKVHDFPSGNGTGGSFSLNRGSQALKFFNTHQLKCQLQRHPDCANVKQWKGGPVKIDPLALVQAIERYLVVRGYGRVREDDEDSDDDGSDEEIDESLAAQFLNSGNVRHRLQFYIGEHLLPYNMTVYQAVRQFSIQAEDERESTDDESNPLGRAGIWTKTHTIWYKPVREDEESNKDCVGGKRGRAQTAPTKTSPRNAKKHDELWHDGVCPSVSNPLEVYLISSPPENITFEDPSLDVILLLRVLHAISRYWYYLYDNAMCKEIIPTSEFINSKLTAKANRQLQDPLVIMTGNIPTWLTELGKTCPFFFPFDTRQMLFYVTAFDRDRAMQRLLDTNPEINQSDSQDSRVAPRLDRKKRTVNREELLKQAESVMQDLGSSRAMLEIQYENEVGTGLGPTLEFYALVSQELQRADLGLWRGEEVTLSNPKGSQEGTKYIQNLQGLFALPFGRTAKPAHIAKVKMKFRFLGKLMAKAIMDFRLVDLPLGLPFYKWMLRQETSLTSHDLFDIDPVVARSVYHLEDIVRQKKRLEQDKSQTKESLQYALETLTMNGCSVEDLGLDFTLPGFPNIELKKGGKDIPVTIHNLEEYLRLVIFWALNEGVSRQFDSFRDGFESVFPLSHLQYFYPEELDQLLCGSKADTWDAKTLMECCRPDHGYTHDSRAVKFLFEILSSFDNEQQRLFLQFVTGSPRLPVGGFRSLNPPLTIVRKTFESTENPDDFLPSVMTCVNYLKLPDYSSIDIMREKLLIAAREGQQSFHLS

Target ORF information:

RefSeq Version	XM_005635800.2
Organism	Canis lupus familiaris(Dog)
Definition	Canis lupus familiaris thyroid hormone receptor interactor 12 (TRIP12), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005635800.2

ORF Insert Sequence:

ATGTCCAACC GGCCTAATAA CAATCCAGGG GGGTCACTGC GACGTTCACA GAGGAACACT 
GCCGGGGCCC AACCACAAGA CGACTCGATA GGAGGAAGGT CACATTTAGG GCAGGCAAAA 
CATAAGGGAT ATAGCCCTCC TGAGAGTAGA AAATCTAATT CTAAGGCACC CAAAGTGCAG 
TCTAATACTA CTTCTGAACT GTCAAGGGGA CACCTTTCTA AAAGAAGCTG CAGTTCATCA 
TCTGCTGTCA TAGTTCCACA ACCAGAGGAT CCAGACAGAG CCAATACTTC AGAAAGGCAA 
AAAACGGGGC AGGTGCCTAA GAAAGACAAT TCTCGAGGAG TGAAACGCAG TGCTAGTCCA 
GACTACAACA GGACCAATTC TCCTAGCTCT GCCAAAAAAC CAAAAGCACT TCAGCATACT 
GAATCTCCCT CAGAAACAAA TAAGCCACAT AGTAAGTCGA AGAAGAGACA TTTAGACCAG 
GAGCAACAAC TGAAATCTGC ACAGTCGCCA TCAACAAGTA AGGCTCACAC CAGAAAGAGT 
GGGGCCACCG GCGGCTCACG GAGTCAGAAA AGAAAGAGGA CAGAGAGTTC TTGTCTAAAG 
AGTGGTTCTG TGTCTGAGTC AACTGGTGCC GAAGAGAGAT CTGCAAAACC CACCAAGCTG 
GCTTCAAAAT CCGCCGCCTC GGCCAAAGCT GGGTGCAGCA CCATCACTGA TTCTTCCTCC 
GCTGCCTCTA CTTCCTCCTC ATCTTCTGCT GTCGCCTCGG CCTCCTCCAC TGTACCACCA 
GGGGCCAGGG TGAAACAAGG AAAAGACCAG AGCAAGGCCA GGCGTTCCCG TTCAGCATCC 
AGTCCCAGTC CCAGAAGAAG TAGCAGGGAA AAGGAACAGA GTAAAACTGG TGGCTCTTCA 
AAATTCGATT GGGCTGCTCG TTTCAGCCCT AAAGTTAGCC TTCCTAAAAC CAAACTGTCT 
CTTCCAGGGT CTTCTAAGTC AGAGACATCA AAACCTGGAC CTTCTGGATT ACAGGCCAAA 
TTAGCAAGTT TAAGAAAATC TACCAAGAAG CGCAGTGAAT CCCCACCTGC TGAGCTCCCC 
AGTTTGAGGC GGAGCACACG CCAAAAGACC ACGGGCTCCT GTGCTAGTGC CAGTCGGCGA 
GGCTCTGGCC TGGGCAAAAG AGGAGCAGCT GAAGCTCGTC GACAGGAGAA GATGGCGGAC 
CCTGAAAGCA GCCAGGAGAC CGTGAATTCT TCAGCTGCAC GGACAGACGA AACTCCCCAA 
GGAGCTGCAG CCTCTAGTTC TGTTGCAGGG GCTGTGGGCA TGACCACCTC TGGGGAGAGT 
GAATCCGATG ATTCTGAGAT GGGACGACTA CAAGCTTTGT TAGAGGCCAG GGGTCTTCCT 
CCTCATCTGT TTGGCCCTCT CGGTCCTCGG ATGTCACAAC TTTTCCATAG AACAATTGGA 
AGCGGAGCTA GCTCTAAGGC CCAGCAGCTT CTGCAAGGAC TACAAGCAAG TGATGAAAGC 
CAGCAACTCC AGGCAGTTAT TGAAATGTGT CAGTTATTGG TTATGGGAAA TGAGGAGACG 
CTGGGAGGGT TTCCGGTCAA GAGTGTCGTT CCAGCTTTGA TAACATTACT GCAGATGGAG 
CATAATTTCG ATATTATGAA CCATGCTTGT CGAGCCTTAA CATATATGAT GGAAGCACTT 
CCTCGGTCAT CTGCTGTTGT AGTAGATGCA ATTCCTGTCT TTTTAGAAAA GCTGCAAGTT 
ATTCAGTGTA TTGATGTCGC AGAGCAGGCC CTGACTGCCT TGGAGATGTT ATCACGCAGA 
CACAGTAAAG CCATTCTACA GGCGGGTGGT TTGGCAGACT GCTTGCTGTA TCTAGAATTC 
TTCAGCATAA ATGCCCAAAG AAATGCACTA GCAATTGCAG CCAATTGCTG CCAGAGTATC 
ACGCCAGATG AATTTCATTT TGTGGCAGAT TCCCTCCCGT TGCTTACCCA AAGGCTAACC 
CATCAGGACA AAAAGTCAGT TGAAAGCACT TGCCTTTGTT TTGCACGTCT AGTGGACAAC 
TTCCAACATG AGGAGAATTT ACTCCAGCAG GTTGCCTCCA AAGATCTGCT TACGAATGTT 
CAACAACTCT TGGTAGTGAC TCCACCCATT TTAAGTTCTG GGATGTTTAT AATGGTGGTT 
CGCATGTTTT CTCTGATGTG TTCCAACTGT CCAACTTTAG CTGTTCAACT TATGAAGCAA 
AACATTGCAG AAACACTTCA CTTTCTCCTG TGTGGTGCCT CTAATGGAAG TTGTCAGGAA 
CAGATTGATC TTGTTCCACG AAGTCCACAA GAACTGTATG AACTGACATC TCTGATTTGC 
GAACTTATGC CATGTTTACC AAAAGAAGGC ATTTTTGCAG TTGATACCAT GCTGAAGAAG 
GGAAATGCGC AGAACACAGA CGGTGCGATT TGGCAGTGGC GTGATGACCG TGGCCTTTGG 
CATCCCTATA ACAGGATTGA CAGCCGGATC ATTGAGGTAG CAGCCCATCA GGTCGGTGAG 
GATGAGATAA GCTTGTCCAC TCTGGGACGA GTTTATACTA TTGATTTTAA TTCTATGCAG 
CAAATCAATG AGGACACGGG AACAGCACGT GCCATTCAGA GAAAACCTAA CCCCTTAGCC 
AATACTAACA CTAGTGGATA TTCAGAGTTA AAGAAGGATG ATGCTCGAGC ACAGCTTATG 
AAAGAGGATC CGGAACTGGC TAAGTCTTTT ATTAAGACAT TATTTGGTGT TCTTTATGAA 
GTGTATAGTT CCTCAGCAGG ACCTGCGGTC AGACATAAGT GCCTTAGAGC AATTCTTAGG 
ATAATTTATT TTGCGGATGC TGAACTTCTG AAGGATGTCC TGAAAAATCA TGCTGTTTCA 
AGTCACATTG CTTCCATGCT GTCAAGCCAA GACCTGAAGA TAGTAGTTGG AGCACTTCAG 
ATGGCAGAAA TCTTAATGCA GAAGTTACCT GATATTTTTA GTGTTTACTT CAGAAGAGAA 
GGTGTAATGC ATCAAGTAAA ACACTTAGCA GAATCAGAGT CTTTGTTGAC AAGTCCACCA 
AAGGCATGTA CGAATGGGTC AGGATCCCTG GGATCCACGA CGTCAGTCAG CAGTGGAACA 
GCCACGGCCG CCACCAATGC TGCAGCTGAC CTGGGGTCCC CCAGCTTGCA GCACAGCAGA 
GATGACTCTT TAGATCTGAG CCCTCAAGGT CGATTAAGTG ATGTTCTAAA GAGAAAACGG 
CTGCCAAAAC GAGGGCCAAG AAGGCCAAAG TATTCACCTC CAAGAGATGA TGACAAAGTA 
GACAATCAAG CTAAAAGCCC TACCACTACT CAGTCACCTA AATCTTCTTT CCTGGCAAGC 
TTGAATCCAA AAACGTGGGG AAGGTTAAGC GCACAGTCCA ACAGCAACAA CATCGAACCA 
GCACGAACTG CGGGAGTCAG TGGTCTTGCC AGGGCCGCCT CCAAGGACAC CATATCCAAT 
AACAGAGAAA AAATTAAAGG TTGGATTAAG GAACAGGCGC ATAAATTTGT AGAGCGTTAT 
TTCAGTTCTG AGAACATGGA TGGAAGCAAC CCTGCACTGA ATGTCCTTCA GAGACTTTGT 
GCTGCAACTG AACAACTCAA CCTCCAGGTG GATGGTGGAG CTGAGTGCCT TGTAGAAATC 
CGTAGCATAG TCTCAGAGTC TGATGTTTCA TCATTTGAAA TCCAACATAG TGGATTTGTG 
AAGCAGCTGT TACTTTATTT GACATCTAAA AGTGAAAAGG ATGCTGTGAG CCGAGAGATC 
AGATTAAAGA GATTCCTTCA CGTATTTTTT TCTTCACCAC TTCCTGGAGA AGAGCCCATT 
GGAAGAGTAG AACCAGTGGG TAATGCGCCT TTGTTGGCAT TAGTTCACAA GATGAACAAC 
TGCCTCAGCC AGATGGAACA GTTTCCAGTC AAAGTACATG ACTTCCCCAG TGGGAACGGG 
ACAGGAGGCA GCTTTTCTCT CAACAGAGGA TCACAGGCTT TAAAATTTTT CAACACACAC 
CAGTTAAAAT GCCAGTTACA AAGACATCCA GACTGTGCAA ATGTGAAGCA GTGGAAGGGC 
GGGCCGGTCA AGATTGATCC CCTGGCTTTG GTACAAGCTA TTGAGAGATA CCTTGTAGTT 
AGAGGGTATG GAAGAGTAAG AGAAGATGAT GAAGACAGTG ATGATGATGG CTCTGATGAG 
GAAATAGACG AGTCTCTGGC CGCTCAGTTC TTAAATTCCG GAAACGTAAG GCACAGGCTA 
CAGTTTTATA TTGGAGAACA TTTGCTACCA TATAACATGA CTGTATATCA GGCAGTCCGG 
CAGTTCAGTA TACAAGCTGA GGATGAGCGG GAGTCCACAG ATGATGAGAG CAACCCTCTG 
GGAAGAGCTG GTATTTGGAC AAAGACTCAT ACAATATGGT ACAAACCTGT GAGAGAGGAT 
GAAGAAAGTA ATAAAGATTG TGTTGGTGGT AAAAGAGGAA GAGCCCAAAC GGCTCCAACA 
AAAACATCCC CTAGAAATGC AAAAAAGCAT GATGAGTTAT GGCATGATGG AGTATGCCCA 
TCAGTATCCA ATCCTTTAGA AGTTTACCTC ATCTCCTCGC CACCTGAGAA TATAACTTTT 
GAAGACCCGT CCTTAGATGT GATTCTTCTT CTAAGAGTTT TACATGCCAT CAGCCGATAC 
TGGTATTACT TGTATGATAA TGCAATGTGC AAGGAGATTA TTCCAACTAG TGAATTTATT 
AACAGTAAGT TGACAGCAAA AGCAAATAGG CAGCTTCAAG ATCCTTTAGT AATCATGACA 
GGAAATATCC CAACGTGGCT TACTGAACTA GGAAAAACCT GCCCATTTTT CTTCCCTTTT 
GATACCCGGC AAATGCTTTT TTACGTAACT GCATTTGATC GGGACCGAGC AATGCAAAGA 
TTACTTGATA CCAACCCAGA AATCAACCAG TCGGATTCTC AAGATAGCAG AGTTGCACCT 
AGATTGGATA GAAAAAAACG TACTGTGAAC AGAGAGGAGC TGCTGAAACA AGCGGAGTCT 
GTAATGCAGG ATCTCGGTAG CTCACGGGCC ATGTTAGAAA TCCAGTATGA AAATGAGGTT 
GGTACAGGTC TTGGGCCTAC ACTGGAGTTT TATGCACTTG TATCTCAGGA ACTTCAGAGA 
GCTGACTTGG GTCTCTGGAG AGGTGAAGAA GTGACTCTCA GCAATCCAAA AGGAAGCCAA 
GAAGGGACCA AGTATATTCA AAACCTCCAG GGCCTGTTTG CACTTCCCTT TGGTAGGACA 
GCTAAGCCAG CCCATATCGC AAAGGTTAAA ATGAAGTTTC GCTTCTTAGG AAAATTAATG 
GCCAAGGCTA TCATGGATTT CAGATTGGTG GACCTTCCCC TTGGTTTACC CTTTTATAAG 
TGGATGCTAA GGCAAGAAAC TTCACTGACG TCGCATGATT TGTTTGACAT TGACCCAGTT 
GTAGCCAGAT CAGTGTATCA CCTAGAAGAC ATTGTCAGAC AGAAGAAAAG ACTTGAGCAA 
GATAAATCCC AGACCAAAGA GAGTCTACAA TATGCATTAG AAACCCTGAC TATGAATGGC 
TGCTCAGTTG AAGATCTAGG ATTGGATTTC ACGCTGCCAG GGTTTCCCAA CATTGAACTG 
AAGAAAGGAG GAAAGGATAT ACCAGTTACC ATCCACAATT TAGAGGAGTA TCTAAGACTG 
GTTATATTCT GGGCACTAAA TGAAGGCGTT TCCAGGCAAT TTGATTCCTT CAGAGATGGA 
TTTGAATCTG TCTTCCCACT TAGTCATCTT CAGTACTTCT ACCCGGAAGA ACTGGATCAG 
CTCTTGTGCG GCAGTAAAGC AGACACGTGG GATGCGAAGA CACTGATGGA GTGCTGCAGG 
CCGGACCACG GTTATACTCA TGACAGTCGG GCTGTGAAGT TCTTGTTTGA GATTCTCAGT 
AGTTTTGATA ATGAGCAGCA GAGGTTGTTT CTCCAGTTTG TGACAGGTAG CCCAAGATTG 
CCTGTTGGAG GCTTCCGGAG TCTGAATCCA CCTCTGACAA TTGTCCGGAA GACCTTCGAG 
TCCACGGAGA ACCCCGACGA CTTCTTGCCC TCTGTAATGA CTTGTGTGAA CTACCTTAAG 
TTGCCGGACT ACTCGAGCAT TGACATCATG CGTGAGAAAC TGTTGATTGC AGCGAGAGAA 
GGGCAGCAGT CATTCCATCT TTCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TRIP12 ( XM_005635801.3 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_005635858.1 Canis lupus familiaris thyroid hormone receptor interactor 12 (TRIP12), transcript variant X2, mRNA.

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CloneID	OCe25761
Clone ID Related Accession (Same CDS sequence)	XM_005635800.3 , XM_005635801.2 , XM_005635800.2 , XM_005635801.3 , XM_022409663.1
Accession Version	XM_005635801.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6207bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-09-04
Organism	Canis lupus familiaris(dog)
Product	E3 ubiquitin-protein ligase TRIP12 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006607.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 5, 2017 this sequence version replaced XM_005635801.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCCAACC GGCCTAATAA CAATCCAGGG GGGTCACTGC GACGTTCACA GAGGAACACT 
GCCGGGGCCC AACCACAAGA CGACTCGATA GGAGGAAGGT CACATTTAGG GCAGGCAAAA 
CATAAGGGAT ATAGCCCTCC TGAGAGTAGA AAATCTAATT CTAAGGCACC CAAAGTGCAG 
TCTAATACTA CTTCTGAACT GTCAAGGGGA CACCTTTCTA AAAGAAGCTG CAGTTCATCA 
TCTGCTGTCA TAGTTCCACA ACCAGAGGAT CCAGACAGAG CCAATACTTC AGAAAGGCAA 
AAAACGGGGC AGGTGCCTAA GAAAGACAAT TCTCGAGGAG TGAAACGCAG TGCTAGTCCA 
GACTACAACA GGACCAATTC TCCTAGCTCT GCCAAAAAAC CAAAAGCACT TCAGCATACT 
GAATCTCCCT CAGAAACAAA TAAGCCACAT AGTAAGTCGA AGAAGAGACA TTTAGACCAG 
GAGCAACAAC TGAAATCTGC ACAGTCGCCA TCAACAAGTA AGGCTCACAC CAGAAAGAGT 
GGGGCCACCG GCGGCTCACG GAGTCAGAAA AGAAAGAGGA CAGAGAGTTC TTGTCTAAAG 
AGTGGTTCTG TGTCTGAGTC AACTGGTGCC GAAGAGAGAT CTGCAAAACC CACCAAGCTG 
GCTTCAAAAT CCGCCGCCTC GGCCAAAGCT GGGTGCAGCA CCATCACTGA TTCTTCCTCC 
GCTGCCTCTA CTTCCTCCTC ATCTTCTGCT GTCGCCTCGG CCTCCTCCAC TGTACCACCA 
GGGGCCAGGG TGAAACAAGG AAAAGACCAG AGCAAGGCCA GGCGTTCCCG TTCAGCATCC 
AGTCCCAGTC CCAGAAGAAG TAGCAGGGAA AAGGAACAGA GTAAAACTGG TGGCTCTTCA 
AAATTCGATT GGGCTGCTCG TTTCAGCCCT AAAGTTAGCC TTCCTAAAAC CAAACTGTCT 
CTTCCAGGGT CTTCTAAGTC AGAGACATCA AAACCTGGAC CTTCTGGATT ACAGGCCAAA 
TTAGCAAGTT TAAGAAAATC TACCAAGAAG CGCAGTGAAT CCCCACCTGC TGAGCTCCCC 
AGTTTGAGGC GGAGCACACG CCAAAAGACC ACGGGCTCCT GTGCTAGTGC CAGTCGGCGA 
GGCTCTGGCC TGGGCAAAAG AGGAGCAGCT GAAGCTCGTC GACAGGAGAA GATGGCGGAC 
CCTGAAAGCA GCCAGGAGAC CGTGAATTCT TCAGCTGCAC GGACAGACGA AACTCCCCAA 
GGAGCTGCAG CCTCTAGTTC TGTTGCAGGG GCTGTGGGCA TGACCACCTC TGGGGAGAGT 
GAATCCGATG ATTCTGAGAT GGGACGACTA CAAGCTTTGT TAGAGGCCAG GGGTCTTCCT 
CCTCATCTGT TTGGCCCTCT CGGTCCTCGG ATGTCACAAC TTTTCCATAG AACAATTGGA 
AGCGGAGCTA GCTCTAAGGC CCAGCAGCTT CTGCAAGGAC TACAAGCAAG TGATGAAAGC 
CAGCAACTCC AGGCAGTTAT TGAAATGTGT CAGTTATTGG TTATGGGAAA TGAGGAGACG 
CTGGGAGGGT TTCCGGTCAA GAGTGTCGTT CCAGCTTTGA TAACATTACT GCAGATGGAG 
CATAATTTCG ATATTATGAA CCATGCTTGT CGAGCCTTAA CATATATGAT GGAAGCACTT 
CCTCGGTCAT CTGCTGTTGT AGTAGATGCA ATTCCTGTCT TTTTAGAAAA GCTGCAAGTT 
ATTCAGTGTA TTGATGTCGC AGAGCAGGCC CTGACTGCCT TGGAGATGTT ATCACGCAGA 
CACAGTAAAG CCATTCTACA GGCGGGTGGT TTGGCAGACT GCTTGCTGTA TCTAGAATTC 
TTCAGCATAA ATGCCCAAAG AAATGCACTA GCAATTGCAG CCAATTGCTG CCAGAGTATC 
ACGCCAGATG AATTTCATTT TGTGGCAGAT TCCCTCCCGT TGCTTACCCA AAGGCTAACC 
CATCAGGACA AAAAGTCAGT TGAAAGCACT TGCCTTTGTT TTGCACGTCT AGTGGACAAC 
TTCCAACATG AGGAGAATTT ACTCCAGCAG GTTGCCTCCA AAGATCTGCT TACGAATGTT 
CAACAACTCT TGGTAGTGAC TCCACCCATT TTAAGTTCTG GGATGTTTAT AATGGTGGTT 
CGCATGTTTT CTCTGATGTG TTCCAACTGT CCAACTTTAG CTGTTCAACT TATGAAGCAA 
AACATTGCAG AAACACTTCA CTTTCTCCTG TGTGGTGCCT CTAATGGAAG TTGTCAGGAA 
CAGATTGATC TTGTTCCACG AAGTCCACAA GAACTGTATG AACTGACATC TCTGATTTGC 
GAACTTATGC CATGTTTACC AAAAGAAGGC ATTTTTGCAG TTGATACCAT GCTGAAGAAG 
GGAAATGCGC AGAACACAGA CGGTGCGATT TGGCAGTGGC GTGATGACCG TGGCCTTTGG 
CATCCCTATA ACAGGATTGA CAGCCGGATC ATTGAGGTAG CAGCCCATCA GGTCGGTGAG 
GATGAGATAA GCTTGTCCAC TCTGGGACGA GTTTATACTA TTGATTTTAA TTCTATGCAG 
CAAATCAATG AGGACACGGG AACAGCACGT GCCATTCAGA GAAAACCTAA CCCCTTAGCC 
AATACTAACA CTAGTGGATA TTCAGAGTTA AAGAAGGATG ATGCTCGAGC ACAGCTTATG 
AAAGAGGATC CGGAACTGGC TAAGTCTTTT ATTAAGACAT TATTTGGTGT TCTTTATGAA 
GTGTATAGTT CCTCAGCAGG ACCTGCGGTC AGACATAAGT GCCTTAGAGC AATTCTTAGG 
ATAATTTATT TTGCGGATGC TGAACTTCTG AAGGATGTCC TGAAAAATCA TGCTGTTTCA 
AGTCACATTG CTTCCATGCT GTCAAGCCAA GACCTGAAGA TAGTAGTTGG AGCACTTCAG 
ATGGCAGAAA TCTTAATGCA GAAGTTACCT GATATTTTTA GTGTTTACTT CAGAAGAGAA 
GGTGTAATGC ATCAAGTAAA ACACTTAGCA GAATCAGAGT CTTTGTTGAC AAGTCCACCA 
AAGGCATGTA CGAATGGGTC AGGATCCCTG GGATCCACGA CGTCAGTCAG CAGTGGAACA 
GCCACGGCCG CCACCAATGC TGCAGCTGAC CTGGGGTCCC CCAGCTTGCA GCACAGCAGA 
GATGACTCTT TAGATCTGAG CCCTCAAGGT CGATTAAGTG ATGTTCTAAA GAGAAAACGG 
CTGCCAAAAC GAGGGCCAAG AAGGCCAAAG TATTCACCTC CAAGAGATGA TGACAAAGTA 
GACAATCAAG CTAAAAGCCC TACCACTACT CAGTCACCTA AATCTTCTTT CCTGGCAAGC 
TTGAATCCAA AAACGTGGGG AAGGTTAAGC GCACAGTCCA ACAGCAACAA CATCGAACCA 
GCACGAACTG CGGGAGTCAG TGGTCTTGCC AGGGCCGCCT CCAAGGACAC CATATCCAAT 
AACAGAGAAA AAATTAAAGG TTGGATTAAG GAACAGGCGC ATAAATTTGT AGAGCGTTAT 
TTCAGTTCTG AGAACATGGA TGGAAGCAAC CCTGCACTGA ATGTCCTTCA GAGACTTTGT 
GCTGCAACTG AACAACTCAA CCTCCAGGTG GATGGTGGAG CTGAGTGCCT TGTAGAAATC 
CGTAGCATAG TCTCAGAGTC TGATGTTTCA TCATTTGAAA TCCAACATAG TGGATTTGTG 
AAGCAGCTGT TACTTTATTT GACATCTAAA AGTGAAAAGG ATGCTGTGAG CCGAGAGATC 
AGATTAAAGA GATTCCTTCA CGTATTTTTT TCTTCACCAC TTCCTGGAGA AGAGCCCATT 
GGAAGAGTAG AACCAGTGGG TAATGCGCCT TTGTTGGCAT TAGTTCACAA GATGAACAAC 
TGCCTCAGCC AGATGGAACA GTTTCCAGTC AAAGTACATG ACTTCCCCAG TGGGAACGGG 
ACAGGAGGCA GCTTTTCTCT CAACAGAGGA TCACAGGCTT TAAAATTTTT CAACACACAC 
CAGTTAAAAT GCCAGTTACA AAGACATCCA GACTGTGCAA ATGTGAAGCA GTGGAAGGGC 
GGGCCGGTCA AGATTGATCC CCTGGCTTTG GTACAAGCTA TTGAGAGATA CCTTGTAGTT 
AGAGGGTATG GAAGAGTAAG AGAAGATGAT GAAGACAGTG ATGATGATGG CTCTGATGAG 
GAAATAGACG AGTCTCTGGC CGCTCAGTTC TTAAATTCCG GAAACGTAAG GCACAGGCTA 
CAGTTTTATA TTGGAGAACA TTTGCTACCA TATAACATGA CTGTATATCA GGCAGTCCGG 
CAGTTCAGTA TACAAGCTGA GGATGAGCGG GAGTCCACAG ATGATGAGAG CAACCCTCTG 
GGAAGAGCTG GTATTTGGAC AAAGACTCAT ACAATATGGT ACAAACCTGT GAGAGAGGAT 
GAAGAAAGTA ATAAAGATTG TGTTGGTGGT AAAAGAGGAA GAGCCCAAAC GGCTCCAACA 
AAAACATCCC CTAGAAATGC AAAAAAGCAT GATGAGTTAT GGCATGATGG AGTATGCCCA 
TCAGTATCCA ATCCTTTAGA AGTTTACCTC ATCTCCTCGC CACCTGAGAA TATAACTTTT 
GAAGACCCGT CCTTAGATGT GATTCTTCTT CTAAGAGTTT TACATGCCAT CAGCCGATAC 
TGGTATTACT TGTATGATAA TGCAATGTGC AAGGAGATTA TTCCAACTAG TGAATTTATT 
AACAGTAAGT TGACAGCAAA AGCAAATAGG CAGCTTCAAG ATCCTTTAGT AATCATGACA 
GGAAATATCC CAACGTGGCT TACTGAACTA GGAAAAACCT GCCCATTTTT CTTCCCTTTT 
GATACCCGGC AAATGCTTTT TTACGTAACT GCATTTGATC GGGACCGAGC AATGCAAAGA 
TTACTTGATA CCAACCCAGA AATCAACCAG TCGGATTCTC AAGATAGCAG AGTTGCACCT 
AGATTGGATA GAAAAAAACG TACTGTGAAC AGAGAGGAGC TGCTGAAACA AGCGGAGTCT 
GTAATGCAGG ATCTCGGTAG CTCACGGGCC ATGTTAGAAA TCCAGTATGA AAATGAGGTT 
GGTACAGGTC TTGGGCCTAC ACTGGAGTTT TATGCACTTG TATCTCAGGA ACTTCAGAGA 
GCTGACTTGG GTCTCTGGAG AGGTGAAGAA GTGACTCTCA GCAATCCAAA AGGAAGCCAA 
GAAGGGACCA AGTATATTCA AAACCTCCAG GGCCTGTTTG CACTTCCCTT TGGTAGGACA 
GCTAAGCCAG CCCATATCGC AAAGGTTAAA ATGAAGTTTC GCTTCTTAGG AAAATTAATG 
GCCAAGGCTA TCATGGATTT CAGATTGGTG GACCTTCCCC TTGGTTTACC CTTTTATAAG 
TGGATGCTAA GGCAAGAAAC TTCACTGACG TCGCATGATT TGTTTGACAT TGACCCAGTT 
GTAGCCAGAT CAGTGTATCA CCTAGAAGAC ATTGTCAGAC AGAAGAAAAG ACTTGAGCAA 
GATAAATCCC AGACCAAAGA GAGTCTACAA TATGCATTAG AAACCCTGAC TATGAATGGC 
TGCTCAGTTG AAGATCTAGG ATTGGATTTC ACGCTGCCAG GGTTTCCCAA CATTGAACTG 
AAGAAAGGAG GAAAGGATAT ACCAGTTACC ATCCACAATT TAGAGGAGTA TCTAAGACTG 
GTTATATTCT GGGCACTAAA TGAAGGCGTT TCCAGGCAAT TTGATTCCTT CAGAGATGGA 
TTTGAATCTG TCTTCCCACT TAGTCATCTT CAGTACTTCT ACCCGGAAGA ACTGGATCAG 
CTCTTGTGCG GCAGTAAAGC AGACACGTGG GATGCGAAGA CACTGATGGA GTGCTGCAGG 
CCGGACCACG GTTATACTCA TGACAGTCGG GCTGTGAAGT TCTTGTTTGA GATTCTCAGT 
AGTTTTGATA ATGAGCAGCA GAGGTTGTTT CTCCAGTTTG TGACAGGTAG CCCAAGATTG 
CCTGTTGGAG GCTTCCGGAG TCTGAATCCA CCTCTGACAA TTGTCCGGAA GACCTTCGAG 
TCCACGGAGA ACCCCGACGA CTTCTTGCCC TCTGTAATGA CTTGTGTGAA CTACCTTAAG 
TTGCCGGACT ACTCGAGCAT TGACATCATG CGTGAGAAAC TGTTGATTGC AGCGAGAGAA 
GGGCAGCAGT CATTCCATCT TTCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005635858.1
CDS	68..6274
Translation	MSNRPNNNPGGSLRRSQRNTAGAQPQDDSIGGRSHLGQAKHKGYSPPESRKSNSKAPKVQSNTTSELSRGHLSKRSCSSSSAVIVPQPEDPDRANTSERQKTGQVPKKDNSRGVKRSASPDYNRTNSPSSAKKPKALQHTESPSETNKPHSKSKKRHLDQEQQLKSAQSPSTSKAHTRKSGATGGSRSQKRKRTESSCLKSGSVSESTGAEERSAKPTKLASKSAASAKAGCSTITDSSSAASTSSSSSAVASASSTVPPGARVKQGKDQSKARRSRSASSPSPRRSSREKEQSKTGGSSKFDWAARFSPKVSLPKTKLSLPGSSKSETSKPGPSGLQAKLASLRKSTKKRSESPPAELPSLRRSTRQKTTGSCASASRRGSGLGKRGAAEARRQEKMADPESSQETVNSSAARTDETPQGAAASSSVAGAVGMTTSGESESDDSEMGRLQALLEARGLPPHLFGPLGPRMSQLFHRTIGSGASSKAQQLLQGLQASDESQQLQAVIEMCQLLVMGNEETLGGFPVKSVVPALITLLQMEHNFDIMNHACRALTYMMEALPRSSAVVVDAIPVFLEKLQVIQCIDVAEQALTALEMLSRRHSKAILQAGGLADCLLYLEFFSINAQRNALAIAANCCQSITPDEFHFVADSLPLLTQRLTHQDKKSVESTCLCFARLVDNFQHEENLLQQVASKDLLTNVQQLLVVTPPILSSGMFIMVVRMFSLMCSNCPTLAVQLMKQNIAETLHFLLCGASNGSCQEQIDLVPRSPQELYELTSLICELMPCLPKEGIFAVDTMLKKGNAQNTDGAIWQWRDDRGLWHPYNRIDSRIIEVAAHQVGEDEISLSTLGRVYTIDFNSMQQINEDTGTARAIQRKPNPLANTNTSGYSELKKDDARAQLMKEDPELAKSFIKTLFGVLYEVYSSSAGPAVRHKCLRAILRIIYFADAELLKDVLKNHAVSSHIASMLSSQDLKIVVGALQMAEILMQKLPDIFSVYFRREGVMHQVKHLAESESLLTSPPKACTNGSGSLGSTTSVSSGTATAATNAAADLGSPSLQHSRDDSLDLSPQGRLSDVLKRKRLPKRGPRRPKYSPPRDDDKVDNQAKSPTTTQSPKSSFLASLNPKTWGRLSAQSNSNNIEPARTAGVSGLARAASKDTISNNREKIKGWIKEQAHKFVERYFSSENMDGSNPALNVLQRLCAATEQLNLQVDGGAECLVEIRSIVSESDVSSFEIQHSGFVKQLLLYLTSKSEKDAVSREIRLKRFLHVFFSSPLPGEEPIGRVEPVGNAPLLALVHKMNNCLSQMEQFPVKVHDFPSGNGTGGSFSLNRGSQALKFFNTHQLKCQLQRHPDCANVKQWKGGPVKIDPLALVQAIERYLVVRGYGRVREDDEDSDDDGSDEEIDESLAAQFLNSGNVRHRLQFYIGEHLLPYNMTVYQAVRQFSIQAEDERESTDDESNPLGRAGIWTKTHTIWYKPVREDEESNKDCVGGKRGRAQTAPTKTSPRNAKKHDELWHDGVCPSVSNPLEVYLISSPPENITFEDPSLDVILLLRVLHAISRYWYYLYDNAMCKEIIPTSEFINSKLTAKANRQLQDPLVIMTGNIPTWLTELGKTCPFFFPFDTRQMLFYVTAFDRDRAMQRLLDTNPEINQSDSQDSRVAPRLDRKKRTVNREELLKQAESVMQDLGSSRAMLEIQYENEVGTGLGPTLEFYALVSQELQRADLGLWRGEEVTLSNPKGSQEGTKYIQNLQGLFALPFGRTAKPAHIAKVKMKFRFLGKLMAKAIMDFRLVDLPLGLPFYKWMLRQETSLTSHDLFDIDPVVARSVYHLEDIVRQKKRLEQDKSQTKESLQYALETLTMNGCSVEDLGLDFTLPGFPNIELKKGGKDIPVTIHNLEEYLRLVIFWALNEGVSRQFDSFRDGFESVFPLSHLQYFYPEELDQLLCGSKADTWDAKTLMECCRPDHGYTHDSRAVKFLFEILSSFDNEQQRLFLQFVTGSPRLPVGGFRSLNPPLTIVRKTFESTENPDDFLPSVMTCVNYLKLPDYSSIDIMREKLLIAAREGQQSFHLS

Target ORF information:

RefSeq Version	XM_005635801.3
Organism	Canis lupus familiaris(dog)
Definition	Canis lupus familiaris thyroid hormone receptor interactor 12 (TRIP12), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005635801.3

ORF Insert Sequence:

ATGTCCAACC GGCCTAATAA CAATCCAGGG GGGTCACTGC GACGTTCACA GAGGAACACT 
GCCGGGGCCC AACCACAAGA CGACTCGATA GGAGGAAGGT CACATTTAGG GCAGGCAAAA 
CATAAGGGAT ATAGCCCTCC TGAGAGTAGA AAATCTAATT CTAAGGCACC CAAAGTGCAG 
TCTAATACTA CTTCTGAACT GTCAAGGGGA CACCTTTCTA AAAGAAGCTG CAGTTCATCA 
TCTGCTGTCA TAGTTCCACA ACCAGAGGAT CCAGACAGAG CCAATACTTC AGAAAGGCAA 
AAAACGGGGC AGGTGCCTAA GAAAGACAAT TCTCGAGGAG TGAAACGCAG TGCTAGTCCA 
GACTACAACA GGACCAATTC TCCTAGCTCT GCCAAAAAAC CAAAAGCACT TCAGCATACT 
GAATCTCCCT CAGAAACAAA TAAGCCACAT AGTAAGTCGA AGAAGAGACA TTTAGACCAG 
GAGCAACAAC TGAAATCTGC ACAGTCGCCA TCAACAAGTA AGGCTCACAC CAGAAAGAGT 
GGGGCCACCG GCGGCTCACG GAGTCAGAAA AGAAAGAGGA CAGAGAGTTC TTGTCTAAAG 
AGTGGTTCTG TGTCTGAGTC AACTGGTGCC GAAGAGAGAT CTGCAAAACC CACCAAGCTG 
GCTTCAAAAT CCGCCGCCTC GGCCAAAGCT GGGTGCAGCA CCATCACTGA TTCTTCCTCC 
GCTGCCTCTA CTTCCTCCTC ATCTTCTGCT GTCGCCTCGG CCTCCTCCAC TGTACCACCA 
GGGGCCAGGG TGAAACAAGG AAAAGACCAG AGCAAGGCCA GGCGTTCCCG TTCAGCATCC 
AGTCCCAGTC CCAGAAGAAG TAGCAGGGAA AAGGAACAGA GTAAAACTGG TGGCTCTTCA 
AAATTCGATT GGGCTGCTCG TTTCAGCCCT AAAGTTAGCC TTCCTAAAAC CAAACTGTCT 
CTTCCAGGGT CTTCTAAGTC AGAGACATCA AAACCTGGAC CTTCTGGATT ACAGGCCAAA 
TTAGCAAGTT TAAGAAAATC TACCAAGAAG CGCAGTGAAT CCCCACCTGC TGAGCTCCCC 
AGTTTGAGGC GGAGCACACG CCAAAAGACC ACGGGCTCCT GTGCTAGTGC CAGTCGGCGA 
GGCTCTGGCC TGGGCAAAAG AGGAGCAGCT GAAGCTCGTC GACAGGAGAA GATGGCGGAC 
CCTGAAAGCA GCCAGGAGAC CGTGAATTCT TCAGCTGCAC GGACAGACGA AACTCCCCAA 
GGAGCTGCAG CCTCTAGTTC TGTTGCAGGG GCTGTGGGCA TGACCACCTC TGGGGAGAGT 
GAATCCGATG ATTCTGAGAT GGGACGACTA CAAGCTTTGT TAGAGGCCAG GGGTCTTCCT 
CCTCATCTGT TTGGCCCTCT CGGTCCTCGG ATGTCACAAC TTTTCCATAG AACAATTGGA 
AGCGGAGCTA GCTCTAAGGC CCAGCAGCTT CTGCAAGGAC TACAAGCAAG TGATGAAAGC 
CAGCAACTCC AGGCAGTTAT TGAAATGTGT CAGTTATTGG TTATGGGAAA TGAGGAGACG 
CTGGGAGGGT TTCCGGTCAA GAGTGTCGTT CCAGCTTTGA TAACATTACT GCAGATGGAG 
CATAATTTCG ATATTATGAA CCATGCTTGT CGAGCCTTAA CATATATGAT GGAAGCACTT 
CCTCGGTCAT CTGCTGTTGT AGTAGATGCA ATTCCTGTCT TTTTAGAAAA GCTGCAAGTT 
ATTCAGTGTA TTGATGTCGC AGAGCAGGCC CTGACTGCCT TGGAGATGTT ATCACGCAGA 
CACAGTAAAG CCATTCTACA GGCGGGTGGT TTGGCAGACT GCTTGCTGTA TCTAGAATTC 
TTCAGCATAA ATGCCCAAAG AAATGCACTA GCAATTGCAG CCAATTGCTG CCAGAGTATC 
ACGCCAGATG AATTTCATTT TGTGGCAGAT TCCCTCCCGT TGCTTACCCA AAGGCTAACC 
CATCAGGACA AAAAGTCAGT TGAAAGCACT TGCCTTTGTT TTGCACGTCT AGTGGACAAC 
TTCCAACATG AGGAGAATTT ACTCCAGCAG GTTGCCTCCA AAGATCTGCT TACGAATGTT 
CAACAACTCT TGGTAGTGAC TCCACCCATT TTAAGTTCTG GGATGTTTAT AATGGTGGTT 
CGCATGTTTT CTCTGATGTG TTCCAACTGT CCAACTTTAG CTGTTCAACT TATGAAGCAA 
AACATTGCAG AAACACTTCA CTTTCTCCTG TGTGGTGCCT CTAATGGAAG TTGTCAGGAA 
CAGATTGATC TTGTTCCACG AAGTCCACAA GAACTGTATG AACTGACATC TCTGATTTGC 
GAACTTATGC CATGTTTACC AAAAGAAGGC ATTTTTGCAG TTGATACCAT GCTGAAGAAG 
GGAAATGCGC AGAACACAGA CGGTGCGATT TGGCAGTGGC GTGATGACCG TGGCCTTTGG 
CATCCCTATA ACAGGATTGA CAGCCGGATC ATTGAGGTAG CAGCCCATCA GGTCGGTGAG 
GATGAGATAA GCTTGTCCAC TCTGGGACGA GTTTATACTA TTGATTTTAA TTCTATGCAG 
CAAATCAATG AGGACACGGG AACAGCACGT GCCATTCAGA GAAAACCTAA CCCCTTAGCC 
AATACTAACA CTAGTGGATA TTCAGAGTTA AAGAAGGATG ATGCTCGAGC ACAGCTTATG 
AAAGAGGATC CGGAACTGGC TAAGTCTTTT ATTAAGACAT TATTTGGTGT TCTTTATGAA 
GTGTATAGTT CCTCAGCAGG ACCTGCGGTC AGACATAAGT GCCTTAGAGC AATTCTTAGG 
ATAATTTATT TTGCGGATGC TGAACTTCTG AAGGATGTCC TGAAAAATCA TGCTGTTTCA 
AGTCACATTG CTTCCATGCT GTCAAGCCAA GACCTGAAGA TAGTAGTTGG AGCACTTCAG 
ATGGCAGAAA TCTTAATGCA GAAGTTACCT GATATTTTTA GTGTTTACTT CAGAAGAGAA 
GGTGTAATGC ATCAAGTAAA ACACTTAGCA GAATCAGAGT CTTTGTTGAC AAGTCCACCA 
AAGGCATGTA CGAATGGGTC AGGATCCCTG GGATCCACGA CGTCAGTCAG CAGTGGAACA 
GCCACGGCCG CCACCAATGC TGCAGCTGAC CTGGGGTCCC CCAGCTTGCA GCACAGCAGA 
GATGACTCTT TAGATCTGAG CCCTCAAGGT CGATTAAGTG ATGTTCTAAA GAGAAAACGG 
CTGCCAAAAC GAGGGCCAAG AAGGCCAAAG TATTCACCTC CAAGAGATGA TGACAAAGTA 
GACAATCAAG CTAAAAGCCC TACCACTACT CAGTCACCTA AATCTTCTTT CCTGGCAAGC 
TTGAATCCAA AAACGTGGGG AAGGTTAAGC GCACAGTCCA ACAGCAACAA CATCGAACCA 
GCACGAACTG CGGGAGTCAG TGGTCTTGCC AGGGCCGCCT CCAAGGACAC CATATCCAAT 
AACAGAGAAA AAATTAAAGG TTGGATTAAG GAACAGGCGC ATAAATTTGT AGAGCGTTAT 
TTCAGTTCTG AGAACATGGA TGGAAGCAAC CCTGCACTGA ATGTCCTTCA GAGACTTTGT 
GCTGCAACTG AACAACTCAA CCTCCAGGTG GATGGTGGAG CTGAGTGCCT TGTAGAAATC 
CGTAGCATAG TCTCAGAGTC TGATGTTTCA TCATTTGAAA TCCAACATAG TGGATTTGTG 
AAGCAGCTGT TACTTTATTT GACATCTAAA AGTGAAAAGG ATGCTGTGAG CCGAGAGATC 
AGATTAAAGA GATTCCTTCA CGTATTTTTT TCTTCACCAC TTCCTGGAGA AGAGCCCATT 
GGAAGAGTAG AACCAGTGGG TAATGCGCCT TTGTTGGCAT TAGTTCACAA GATGAACAAC 
TGCCTCAGCC AGATGGAACA GTTTCCAGTC AAAGTACATG ACTTCCCCAG TGGGAACGGG 
ACAGGAGGCA GCTTTTCTCT CAACAGAGGA TCACAGGCTT TAAAATTTTT CAACACACAC 
CAGTTAAAAT GCCAGTTACA AAGACATCCA GACTGTGCAA ATGTGAAGCA GTGGAAGGGC 
GGGCCGGTCA AGATTGATCC CCTGGCTTTG GTACAAGCTA TTGAGAGATA CCTTGTAGTT 
AGAGGGTATG GAAGAGTAAG AGAAGATGAT GAAGACAGTG ATGATGATGG CTCTGATGAG 
GAAATAGACG AGTCTCTGGC CGCTCAGTTC TTAAATTCCG GAAACGTAAG GCACAGGCTA 
CAGTTTTATA TTGGAGAACA TTTGCTACCA TATAACATGA CTGTATATCA GGCAGTCCGG 
CAGTTCAGTA TACAAGCTGA GGATGAGCGG GAGTCCACAG ATGATGAGAG CAACCCTCTG 
GGAAGAGCTG GTATTTGGAC AAAGACTCAT ACAATATGGT ACAAACCTGT GAGAGAGGAT 
GAAGAAAGTA ATAAAGATTG TGTTGGTGGT AAAAGAGGAA GAGCCCAAAC GGCTCCAACA 
AAAACATCCC CTAGAAATGC AAAAAAGCAT GATGAGTTAT GGCATGATGG AGTATGCCCA 
TCAGTATCCA ATCCTTTAGA AGTTTACCTC ATCTCCTCGC CACCTGAGAA TATAACTTTT 
GAAGACCCGT CCTTAGATGT GATTCTTCTT CTAAGAGTTT TACATGCCAT CAGCCGATAC 
TGGTATTACT TGTATGATAA TGCAATGTGC AAGGAGATTA TTCCAACTAG TGAATTTATT 
AACAGTAAGT TGACAGCAAA AGCAAATAGG CAGCTTCAAG ATCCTTTAGT AATCATGACA 
GGAAATATCC CAACGTGGCT TACTGAACTA GGAAAAACCT GCCCATTTTT CTTCCCTTTT 
GATACCCGGC AAATGCTTTT TTACGTAACT GCATTTGATC GGGACCGAGC AATGCAAAGA 
TTACTTGATA CCAACCCAGA AATCAACCAG TCGGATTCTC AAGATAGCAG AGTTGCACCT 
AGATTGGATA GAAAAAAACG TACTGTGAAC AGAGAGGAGC TGCTGAAACA AGCGGAGTCT 
GTAATGCAGG ATCTCGGTAG CTCACGGGCC ATGTTAGAAA TCCAGTATGA AAATGAGGTT 
GGTACAGGTC TTGGGCCTAC ACTGGAGTTT TATGCACTTG TATCTCAGGA ACTTCAGAGA 
GCTGACTTGG GTCTCTGGAG AGGTGAAGAA GTGACTCTCA GCAATCCAAA AGGAAGCCAA 
GAAGGGACCA AGTATATTCA AAACCTCCAG GGCCTGTTTG CACTTCCCTT TGGTAGGACA 
GCTAAGCCAG CCCATATCGC AAAGGTTAAA ATGAAGTTTC GCTTCTTAGG AAAATTAATG 
GCCAAGGCTA TCATGGATTT CAGATTGGTG GACCTTCCCC TTGGTTTACC CTTTTATAAG 
TGGATGCTAA GGCAAGAAAC TTCACTGACG TCGCATGATT TGTTTGACAT TGACCCAGTT 
GTAGCCAGAT CAGTGTATCA CCTAGAAGAC ATTGTCAGAC AGAAGAAAAG ACTTGAGCAA 
GATAAATCCC AGACCAAAGA GAGTCTACAA TATGCATTAG AAACCCTGAC TATGAATGGC 
TGCTCAGTTG AAGATCTAGG ATTGGATTTC ACGCTGCCAG GGTTTCCCAA CATTGAACTG 
AAGAAAGGAG GAAAGGATAT ACCAGTTACC ATCCACAATT TAGAGGAGTA TCTAAGACTG 
GTTATATTCT GGGCACTAAA TGAAGGCGTT TCCAGGCAAT TTGATTCCTT CAGAGATGGA 
TTTGAATCTG TCTTCCCACT TAGTCATCTT CAGTACTTCT ACCCGGAAGA ACTGGATCAG 
CTCTTGTGCG GCAGTAAAGC AGACACGTGG GATGCGAAGA CACTGATGGA GTGCTGCAGG 
CCGGACCACG GTTATACTCA TGACAGTCGG GCTGTGAAGT TCTTGTTTGA GATTCTCAGT 
AGTTTTGATA ATGAGCAGCA GAGGTTGTTT CTCCAGTTTG TGACAGGTAG CCCAAGATTG 
CCTGTTGGAG GCTTCCGGAG TCTGAATCCA CCTCTGACAA TTGTCCGGAA GACCTTCGAG 
TCCACGGAGA ACCCCGACGA CTTCTTGCCC TCTGTAATGA CTTGTGTGAA CTACCTTAAG 
TTGCCGGACT ACTCGAGCAT TGACATCATG CGTGAGAAAC TGTTGATTGC AGCGAGAGAA 
GGGCAGCAGT CATTCCATCT TTCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TRIP12 ( XM_022409663.1 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_022265371.1 Canis lupus familiaris thyroid hormone receptor interactor 12 (TRIP12), transcript variant X3, mRNA.

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CloneID	OCe25761
Clone ID Related Accession (Same CDS sequence)	XM_005635800.3 , XM_005635801.2 , XM_005635800.2 , XM_005635801.3 , XM_022409663.1
Accession Version	XM_022409663.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6207bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-09-04
Organism	Canis lupus familiaris(dog)
Product	E3 ubiquitin-protein ligase TRIP12 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006607.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCCAACC GGCCTAATAA CAATCCAGGG GGGTCACTGC GACGTTCACA GAGGAACACT 
GCCGGGGCCC AACCACAAGA CGACTCGATA GGAGGAAGGT CACATTTAGG GCAGGCAAAA 
CATAAGGGAT ATAGCCCTCC TGAGAGTAGA AAATCTAATT CTAAGGCACC CAAAGTGCAG 
TCTAATACTA CTTCTGAACT GTCAAGGGGA CACCTTTCTA AAAGAAGCTG CAGTTCATCA 
TCTGCTGTCA TAGTTCCACA ACCAGAGGAT CCAGACAGAG CCAATACTTC AGAAAGGCAA 
AAAACGGGGC AGGTGCCTAA GAAAGACAAT TCTCGAGGAG TGAAACGCAG TGCTAGTCCA 
GACTACAACA GGACCAATTC TCCTAGCTCT GCCAAAAAAC CAAAAGCACT TCAGCATACT 
GAATCTCCCT CAGAAACAAA TAAGCCACAT AGTAAGTCGA AGAAGAGACA TTTAGACCAG 
GAGCAACAAC TGAAATCTGC ACAGTCGCCA TCAACAAGTA AGGCTCACAC CAGAAAGAGT 
GGGGCCACCG GCGGCTCACG GAGTCAGAAA AGAAAGAGGA CAGAGAGTTC TTGTCTAAAG 
AGTGGTTCTG TGTCTGAGTC AACTGGTGCC GAAGAGAGAT CTGCAAAACC CACCAAGCTG 
GCTTCAAAAT CCGCCGCCTC GGCCAAAGCT GGGTGCAGCA CCATCACTGA TTCTTCCTCC 
GCTGCCTCTA CTTCCTCCTC ATCTTCTGCT GTCGCCTCGG CCTCCTCCAC TGTACCACCA 
GGGGCCAGGG TGAAACAAGG AAAAGACCAG AGCAAGGCCA GGCGTTCCCG TTCAGCATCC 
AGTCCCAGTC CCAGAAGAAG TAGCAGGGAA AAGGAACAGA GTAAAACTGG TGGCTCTTCA 
AAATTCGATT GGGCTGCTCG TTTCAGCCCT AAAGTTAGCC TTCCTAAAAC CAAACTGTCT 
CTTCCAGGGT CTTCTAAGTC AGAGACATCA AAACCTGGAC CTTCTGGATT ACAGGCCAAA 
TTAGCAAGTT TAAGAAAATC TACCAAGAAG CGCAGTGAAT CCCCACCTGC TGAGCTCCCC 
AGTTTGAGGC GGAGCACACG CCAAAAGACC ACGGGCTCCT GTGCTAGTGC CAGTCGGCGA 
GGCTCTGGCC TGGGCAAAAG AGGAGCAGCT GAAGCTCGTC GACAGGAGAA GATGGCGGAC 
CCTGAAAGCA GCCAGGAGAC CGTGAATTCT TCAGCTGCAC GGACAGACGA AACTCCCCAA 
GGAGCTGCAG CCTCTAGTTC TGTTGCAGGG GCTGTGGGCA TGACCACCTC TGGGGAGAGT 
GAATCCGATG ATTCTGAGAT GGGACGACTA CAAGCTTTGT TAGAGGCCAG GGGTCTTCCT 
CCTCATCTGT TTGGCCCTCT CGGTCCTCGG ATGTCACAAC TTTTCCATAG AACAATTGGA 
AGCGGAGCTA GCTCTAAGGC CCAGCAGCTT CTGCAAGGAC TACAAGCAAG TGATGAAAGC 
CAGCAACTCC AGGCAGTTAT TGAAATGTGT CAGTTATTGG TTATGGGAAA TGAGGAGACG 
CTGGGAGGGT TTCCGGTCAA GAGTGTCGTT CCAGCTTTGA TAACATTACT GCAGATGGAG 
CATAATTTCG ATATTATGAA CCATGCTTGT CGAGCCTTAA CATATATGAT GGAAGCACTT 
CCTCGGTCAT CTGCTGTTGT AGTAGATGCA ATTCCTGTCT TTTTAGAAAA GCTGCAAGTT 
ATTCAGTGTA TTGATGTCGC AGAGCAGGCC CTGACTGCCT TGGAGATGTT ATCACGCAGA 
CACAGTAAAG CCATTCTACA GGCGGGTGGT TTGGCAGACT GCTTGCTGTA TCTAGAATTC 
TTCAGCATAA ATGCCCAAAG AAATGCACTA GCAATTGCAG CCAATTGCTG CCAGAGTATC 
ACGCCAGATG AATTTCATTT TGTGGCAGAT TCCCTCCCGT TGCTTACCCA AAGGCTAACC 
CATCAGGACA AAAAGTCAGT TGAAAGCACT TGCCTTTGTT TTGCACGTCT AGTGGACAAC 
TTCCAACATG AGGAGAATTT ACTCCAGCAG GTTGCCTCCA AAGATCTGCT TACGAATGTT 
CAACAACTCT TGGTAGTGAC TCCACCCATT TTAAGTTCTG GGATGTTTAT AATGGTGGTT 
CGCATGTTTT CTCTGATGTG TTCCAACTGT CCAACTTTAG CTGTTCAACT TATGAAGCAA 
AACATTGCAG AAACACTTCA CTTTCTCCTG TGTGGTGCCT CTAATGGAAG TTGTCAGGAA 
CAGATTGATC TTGTTCCACG AAGTCCACAA GAACTGTATG AACTGACATC TCTGATTTGC 
GAACTTATGC CATGTTTACC AAAAGAAGGC ATTTTTGCAG TTGATACCAT GCTGAAGAAG 
GGAAATGCGC AGAACACAGA CGGTGCGATT TGGCAGTGGC GTGATGACCG TGGCCTTTGG 
CATCCCTATA ACAGGATTGA CAGCCGGATC ATTGAGGTAG CAGCCCATCA GGTCGGTGAG 
GATGAGATAA GCTTGTCCAC TCTGGGACGA GTTTATACTA TTGATTTTAA TTCTATGCAG 
CAAATCAATG AGGACACGGG AACAGCACGT GCCATTCAGA GAAAACCTAA CCCCTTAGCC 
AATACTAACA CTAGTGGATA TTCAGAGTTA AAGAAGGATG ATGCTCGAGC ACAGCTTATG 
AAAGAGGATC CGGAACTGGC TAAGTCTTTT ATTAAGACAT TATTTGGTGT TCTTTATGAA 
GTGTATAGTT CCTCAGCAGG ACCTGCGGTC AGACATAAGT GCCTTAGAGC AATTCTTAGG 
ATAATTTATT TTGCGGATGC TGAACTTCTG AAGGATGTCC TGAAAAATCA TGCTGTTTCA 
AGTCACATTG CTTCCATGCT GTCAAGCCAA GACCTGAAGA TAGTAGTTGG AGCACTTCAG 
ATGGCAGAAA TCTTAATGCA GAAGTTACCT GATATTTTTA GTGTTTACTT CAGAAGAGAA 
GGTGTAATGC ATCAAGTAAA ACACTTAGCA GAATCAGAGT CTTTGTTGAC AAGTCCACCA 
AAGGCATGTA CGAATGGGTC AGGATCCCTG GGATCCACGA CGTCAGTCAG CAGTGGAACA 
GCCACGGCCG CCACCAATGC TGCAGCTGAC CTGGGGTCCC CCAGCTTGCA GCACAGCAGA 
GATGACTCTT TAGATCTGAG CCCTCAAGGT CGATTAAGTG ATGTTCTAAA GAGAAAACGG 
CTGCCAAAAC GAGGGCCAAG AAGGCCAAAG TATTCACCTC CAAGAGATGA TGACAAAGTA 
GACAATCAAG CTAAAAGCCC TACCACTACT CAGTCACCTA AATCTTCTTT CCTGGCAAGC 
TTGAATCCAA AAACGTGGGG AAGGTTAAGC GCACAGTCCA ACAGCAACAA CATCGAACCA 
GCACGAACTG CGGGAGTCAG TGGTCTTGCC AGGGCCGCCT CCAAGGACAC CATATCCAAT 
AACAGAGAAA AAATTAAAGG TTGGATTAAG GAACAGGCGC ATAAATTTGT AGAGCGTTAT 
TTCAGTTCTG AGAACATGGA TGGAAGCAAC CCTGCACTGA ATGTCCTTCA GAGACTTTGT 
GCTGCAACTG AACAACTCAA CCTCCAGGTG GATGGTGGAG CTGAGTGCCT TGTAGAAATC 
CGTAGCATAG TCTCAGAGTC TGATGTTTCA TCATTTGAAA TCCAACATAG TGGATTTGTG 
AAGCAGCTGT TACTTTATTT GACATCTAAA AGTGAAAAGG ATGCTGTGAG CCGAGAGATC 
AGATTAAAGA GATTCCTTCA CGTATTTTTT TCTTCACCAC TTCCTGGAGA AGAGCCCATT 
GGAAGAGTAG AACCAGTGGG TAATGCGCCT TTGTTGGCAT TAGTTCACAA GATGAACAAC 
TGCCTCAGCC AGATGGAACA GTTTCCAGTC AAAGTACATG ACTTCCCCAG TGGGAACGGG 
ACAGGAGGCA GCTTTTCTCT CAACAGAGGA TCACAGGCTT TAAAATTTTT CAACACACAC 
CAGTTAAAAT GCCAGTTACA AAGACATCCA GACTGTGCAA ATGTGAAGCA GTGGAAGGGC 
GGGCCGGTCA AGATTGATCC CCTGGCTTTG GTACAAGCTA TTGAGAGATA CCTTGTAGTT 
AGAGGGTATG GAAGAGTAAG AGAAGATGAT GAAGACAGTG ATGATGATGG CTCTGATGAG 
GAAATAGACG AGTCTCTGGC CGCTCAGTTC TTAAATTCCG GAAACGTAAG GCACAGGCTA 
CAGTTTTATA TTGGAGAACA TTTGCTACCA TATAACATGA CTGTATATCA GGCAGTCCGG 
CAGTTCAGTA TACAAGCTGA GGATGAGCGG GAGTCCACAG ATGATGAGAG CAACCCTCTG 
GGAAGAGCTG GTATTTGGAC AAAGACTCAT ACAATATGGT ACAAACCTGT GAGAGAGGAT 
GAAGAAAGTA ATAAAGATTG TGTTGGTGGT AAAAGAGGAA GAGCCCAAAC GGCTCCAACA 
AAAACATCCC CTAGAAATGC AAAAAAGCAT GATGAGTTAT GGCATGATGG AGTATGCCCA 
TCAGTATCCA ATCCTTTAGA AGTTTACCTC ATCTCCTCGC CACCTGAGAA TATAACTTTT 
GAAGACCCGT CCTTAGATGT GATTCTTCTT CTAAGAGTTT TACATGCCAT CAGCCGATAC 
TGGTATTACT TGTATGATAA TGCAATGTGC AAGGAGATTA TTCCAACTAG TGAATTTATT 
AACAGTAAGT TGACAGCAAA AGCAAATAGG CAGCTTCAAG ATCCTTTAGT AATCATGACA 
GGAAATATCC CAACGTGGCT TACTGAACTA GGAAAAACCT GCCCATTTTT CTTCCCTTTT 
GATACCCGGC AAATGCTTTT TTACGTAACT GCATTTGATC GGGACCGAGC AATGCAAAGA 
TTACTTGATA CCAACCCAGA AATCAACCAG TCGGATTCTC AAGATAGCAG AGTTGCACCT 
AGATTGGATA GAAAAAAACG TACTGTGAAC AGAGAGGAGC TGCTGAAACA AGCGGAGTCT 
GTAATGCAGG ATCTCGGTAG CTCACGGGCC ATGTTAGAAA TCCAGTATGA AAATGAGGTT 
GGTACAGGTC TTGGGCCTAC ACTGGAGTTT TATGCACTTG TATCTCAGGA ACTTCAGAGA 
GCTGACTTGG GTCTCTGGAG AGGTGAAGAA GTGACTCTCA GCAATCCAAA AGGAAGCCAA 
GAAGGGACCA AGTATATTCA AAACCTCCAG GGCCTGTTTG CACTTCCCTT TGGTAGGACA 
GCTAAGCCAG CCCATATCGC AAAGGTTAAA ATGAAGTTTC GCTTCTTAGG AAAATTAATG 
GCCAAGGCTA TCATGGATTT CAGATTGGTG GACCTTCCCC TTGGTTTACC CTTTTATAAG 
TGGATGCTAA GGCAAGAAAC TTCACTGACG TCGCATGATT TGTTTGACAT TGACCCAGTT 
GTAGCCAGAT CAGTGTATCA CCTAGAAGAC ATTGTCAGAC AGAAGAAAAG ACTTGAGCAA 
GATAAATCCC AGACCAAAGA GAGTCTACAA TATGCATTAG AAACCCTGAC TATGAATGGC 
TGCTCAGTTG AAGATCTAGG ATTGGATTTC ACGCTGCCAG GGTTTCCCAA CATTGAACTG 
AAGAAAGGAG GAAAGGATAT ACCAGTTACC ATCCACAATT TAGAGGAGTA TCTAAGACTG 
GTTATATTCT GGGCACTAAA TGAAGGCGTT TCCAGGCAAT TTGATTCCTT CAGAGATGGA 
TTTGAATCTG TCTTCCCACT TAGTCATCTT CAGTACTTCT ACCCGGAAGA ACTGGATCAG 
CTCTTGTGCG GCAGTAAAGC AGACACGTGG GATGCGAAGA CACTGATGGA GTGCTGCAGG 
CCGGACCACG GTTATACTCA TGACAGTCGG GCTGTGAAGT TCTTGTTTGA GATTCTCAGT 
AGTTTTGATA ATGAGCAGCA GAGGTTGTTT CTCCAGTTTG TGACAGGTAG CCCAAGATTG 
CCTGTTGGAG GCTTCCGGAG TCTGAATCCA CCTCTGACAA TTGTCCGGAA GACCTTCGAG 
TCCACGGAGA ACCCCGACGA CTTCTTGCCC TCTGTAATGA CTTGTGTGAA CTACCTTAAG 
TTGCCGGACT ACTCGAGCAT TGACATCATG CGTGAGAAAC TGTTGATTGC AGCGAGAGAA 
GGGCAGCAGT CATTCCATCT TTCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_022265371.1
CDS	1..6207
Translation	MSNRPNNNPGGSLRRSQRNTAGAQPQDDSIGGRSHLGQAKHKGYSPPESRKSNSKAPKVQSNTTSELSRGHLSKRSCSSSSAVIVPQPEDPDRANTSERQKTGQVPKKDNSRGVKRSASPDYNRTNSPSSAKKPKALQHTESPSETNKPHSKSKKRHLDQEQQLKSAQSPSTSKAHTRKSGATGGSRSQKRKRTESSCLKSGSVSESTGAEERSAKPTKLASKSAASAKAGCSTITDSSSAASTSSSSSAVASASSTVPPGARVKQGKDQSKARRSRSASSPSPRRSSREKEQSKTGGSSKFDWAARFSPKVSLPKTKLSLPGSSKSETSKPGPSGLQAKLASLRKSTKKRSESPPAELPSLRRSTRQKTTGSCASASRRGSGLGKRGAAEARRQEKMADPESSQETVNSSAARTDETPQGAAASSSVAGAVGMTTSGESESDDSEMGRLQALLEARGLPPHLFGPLGPRMSQLFHRTIGSGASSKAQQLLQGLQASDESQQLQAVIEMCQLLVMGNEETLGGFPVKSVVPALITLLQMEHNFDIMNHACRALTYMMEALPRSSAVVVDAIPVFLEKLQVIQCIDVAEQALTALEMLSRRHSKAILQAGGLADCLLYLEFFSINAQRNALAIAANCCQSITPDEFHFVADSLPLLTQRLTHQDKKSVESTCLCFARLVDNFQHEENLLQQVASKDLLTNVQQLLVVTPPILSSGMFIMVVRMFSLMCSNCPTLAVQLMKQNIAETLHFLLCGASNGSCQEQIDLVPRSPQELYELTSLICELMPCLPKEGIFAVDTMLKKGNAQNTDGAIWQWRDDRGLWHPYNRIDSRIIEVAAHQVGEDEISLSTLGRVYTIDFNSMQQINEDTGTARAIQRKPNPLANTNTSGYSELKKDDARAQLMKEDPELAKSFIKTLFGVLYEVYSSSAGPAVRHKCLRAILRIIYFADAELLKDVLKNHAVSSHIASMLSSQDLKIVVGALQMAEILMQKLPDIFSVYFRREGVMHQVKHLAESESLLTSPPKACTNGSGSLGSTTSVSSGTATAATNAAADLGSPSLQHSRDDSLDLSPQGRLSDVLKRKRLPKRGPRRPKYSPPRDDDKVDNQAKSPTTTQSPKSSFLASLNPKTWGRLSAQSNSNNIEPARTAGVSGLARAASKDTISNNREKIKGWIKEQAHKFVERYFSSENMDGSNPALNVLQRLCAATEQLNLQVDGGAECLVEIRSIVSESDVSSFEIQHSGFVKQLLLYLTSKSEKDAVSREIRLKRFLHVFFSSPLPGEEPIGRVEPVGNAPLLALVHKMNNCLSQMEQFPVKVHDFPSGNGTGGSFSLNRGSQALKFFNTHQLKCQLQRHPDCANVKQWKGGPVKIDPLALVQAIERYLVVRGYGRVREDDEDSDDDGSDEEIDESLAAQFLNSGNVRHRLQFYIGEHLLPYNMTVYQAVRQFSIQAEDERESTDDESNPLGRAGIWTKTHTIWYKPVREDEESNKDCVGGKRGRAQTAPTKTSPRNAKKHDELWHDGVCPSVSNPLEVYLISSPPENITFEDPSLDVILLLRVLHAISRYWYYLYDNAMCKEIIPTSEFINSKLTAKANRQLQDPLVIMTGNIPTWLTELGKTCPFFFPFDTRQMLFYVTAFDRDRAMQRLLDTNPEINQSDSQDSRVAPRLDRKKRTVNREELLKQAESVMQDLGSSRAMLEIQYENEVGTGLGPTLEFYALVSQELQRADLGLWRGEEVTLSNPKGSQEGTKYIQNLQGLFALPFGRTAKPAHIAKVKMKFRFLGKLMAKAIMDFRLVDLPLGLPFYKWMLRQETSLTSHDLFDIDPVVARSVYHLEDIVRQKKRLEQDKSQTKESLQYALETLTMNGCSVEDLGLDFTLPGFPNIELKKGGKDIPVTIHNLEEYLRLVIFWALNEGVSRQFDSFRDGFESVFPLSHLQYFYPEELDQLLCGSKADTWDAKTLMECCRPDHGYTHDSRAVKFLFEILSSFDNEQQRLFLQFVTGSPRLPVGGFRSLNPPLTIVRKTFESTENPDDFLPSVMTCVNYLKLPDYSSIDIMREKLLIAAREGQQSFHLS

Target ORF information:

RefSeq Version	XM_022409663.1
Organism	Canis lupus familiaris(dog)
Definition	Canis lupus familiaris thyroid hormone receptor interactor 12 (TRIP12), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_022409663.1

ORF Insert Sequence:

ATGTCCAACC GGCCTAATAA CAATCCAGGG GGGTCACTGC GACGTTCACA GAGGAACACT 
GCCGGGGCCC AACCACAAGA CGACTCGATA GGAGGAAGGT CACATTTAGG GCAGGCAAAA 
CATAAGGGAT ATAGCCCTCC TGAGAGTAGA AAATCTAATT CTAAGGCACC CAAAGTGCAG 
TCTAATACTA CTTCTGAACT GTCAAGGGGA CACCTTTCTA AAAGAAGCTG CAGTTCATCA 
TCTGCTGTCA TAGTTCCACA ACCAGAGGAT CCAGACAGAG CCAATACTTC AGAAAGGCAA 
AAAACGGGGC AGGTGCCTAA GAAAGACAAT TCTCGAGGAG TGAAACGCAG TGCTAGTCCA 
GACTACAACA GGACCAATTC TCCTAGCTCT GCCAAAAAAC CAAAAGCACT TCAGCATACT 
GAATCTCCCT CAGAAACAAA TAAGCCACAT AGTAAGTCGA AGAAGAGACA TTTAGACCAG 
GAGCAACAAC TGAAATCTGC ACAGTCGCCA TCAACAAGTA AGGCTCACAC CAGAAAGAGT 
GGGGCCACCG GCGGCTCACG GAGTCAGAAA AGAAAGAGGA CAGAGAGTTC TTGTCTAAAG 
AGTGGTTCTG TGTCTGAGTC AACTGGTGCC GAAGAGAGAT CTGCAAAACC CACCAAGCTG 
GCTTCAAAAT CCGCCGCCTC GGCCAAAGCT GGGTGCAGCA CCATCACTGA TTCTTCCTCC 
GCTGCCTCTA CTTCCTCCTC ATCTTCTGCT GTCGCCTCGG CCTCCTCCAC TGTACCACCA 
GGGGCCAGGG TGAAACAAGG AAAAGACCAG AGCAAGGCCA GGCGTTCCCG TTCAGCATCC 
AGTCCCAGTC CCAGAAGAAG TAGCAGGGAA AAGGAACAGA GTAAAACTGG TGGCTCTTCA 
AAATTCGATT GGGCTGCTCG TTTCAGCCCT AAAGTTAGCC TTCCTAAAAC CAAACTGTCT 
CTTCCAGGGT CTTCTAAGTC AGAGACATCA AAACCTGGAC CTTCTGGATT ACAGGCCAAA 
TTAGCAAGTT TAAGAAAATC TACCAAGAAG CGCAGTGAAT CCCCACCTGC TGAGCTCCCC 
AGTTTGAGGC GGAGCACACG CCAAAAGACC ACGGGCTCCT GTGCTAGTGC CAGTCGGCGA 
GGCTCTGGCC TGGGCAAAAG AGGAGCAGCT GAAGCTCGTC GACAGGAGAA GATGGCGGAC 
CCTGAAAGCA GCCAGGAGAC CGTGAATTCT TCAGCTGCAC GGACAGACGA AACTCCCCAA 
GGAGCTGCAG CCTCTAGTTC TGTTGCAGGG GCTGTGGGCA TGACCACCTC TGGGGAGAGT 
GAATCCGATG ATTCTGAGAT GGGACGACTA CAAGCTTTGT TAGAGGCCAG GGGTCTTCCT 
CCTCATCTGT TTGGCCCTCT CGGTCCTCGG ATGTCACAAC TTTTCCATAG AACAATTGGA 
AGCGGAGCTA GCTCTAAGGC CCAGCAGCTT CTGCAAGGAC TACAAGCAAG TGATGAAAGC 
CAGCAACTCC AGGCAGTTAT TGAAATGTGT CAGTTATTGG TTATGGGAAA TGAGGAGACG 
CTGGGAGGGT TTCCGGTCAA GAGTGTCGTT CCAGCTTTGA TAACATTACT GCAGATGGAG 
CATAATTTCG ATATTATGAA CCATGCTTGT CGAGCCTTAA CATATATGAT GGAAGCACTT 
CCTCGGTCAT CTGCTGTTGT AGTAGATGCA ATTCCTGTCT TTTTAGAAAA GCTGCAAGTT 
ATTCAGTGTA TTGATGTCGC AGAGCAGGCC CTGACTGCCT TGGAGATGTT ATCACGCAGA 
CACAGTAAAG CCATTCTACA GGCGGGTGGT TTGGCAGACT GCTTGCTGTA TCTAGAATTC 
TTCAGCATAA ATGCCCAAAG AAATGCACTA GCAATTGCAG CCAATTGCTG CCAGAGTATC 
ACGCCAGATG AATTTCATTT TGTGGCAGAT TCCCTCCCGT TGCTTACCCA AAGGCTAACC 
CATCAGGACA AAAAGTCAGT TGAAAGCACT TGCCTTTGTT TTGCACGTCT AGTGGACAAC 
TTCCAACATG AGGAGAATTT ACTCCAGCAG GTTGCCTCCA AAGATCTGCT TACGAATGTT 
CAACAACTCT TGGTAGTGAC TCCACCCATT TTAAGTTCTG GGATGTTTAT AATGGTGGTT 
CGCATGTTTT CTCTGATGTG TTCCAACTGT CCAACTTTAG CTGTTCAACT TATGAAGCAA 
AACATTGCAG AAACACTTCA CTTTCTCCTG TGTGGTGCCT CTAATGGAAG TTGTCAGGAA 
CAGATTGATC TTGTTCCACG AAGTCCACAA GAACTGTATG AACTGACATC TCTGATTTGC 
GAACTTATGC CATGTTTACC AAAAGAAGGC ATTTTTGCAG TTGATACCAT GCTGAAGAAG 
GGAAATGCGC AGAACACAGA CGGTGCGATT TGGCAGTGGC GTGATGACCG TGGCCTTTGG 
CATCCCTATA ACAGGATTGA CAGCCGGATC ATTGAGGTAG CAGCCCATCA GGTCGGTGAG 
GATGAGATAA GCTTGTCCAC TCTGGGACGA GTTTATACTA TTGATTTTAA TTCTATGCAG 
CAAATCAATG AGGACACGGG AACAGCACGT GCCATTCAGA GAAAACCTAA CCCCTTAGCC 
AATACTAACA CTAGTGGATA TTCAGAGTTA AAGAAGGATG ATGCTCGAGC ACAGCTTATG 
AAAGAGGATC CGGAACTGGC TAAGTCTTTT ATTAAGACAT TATTTGGTGT TCTTTATGAA 
GTGTATAGTT CCTCAGCAGG ACCTGCGGTC AGACATAAGT GCCTTAGAGC AATTCTTAGG 
ATAATTTATT TTGCGGATGC TGAACTTCTG AAGGATGTCC TGAAAAATCA TGCTGTTTCA 
AGTCACATTG CTTCCATGCT GTCAAGCCAA GACCTGAAGA TAGTAGTTGG AGCACTTCAG 
ATGGCAGAAA TCTTAATGCA GAAGTTACCT GATATTTTTA GTGTTTACTT CAGAAGAGAA 
GGTGTAATGC ATCAAGTAAA ACACTTAGCA GAATCAGAGT CTTTGTTGAC AAGTCCACCA 
AAGGCATGTA CGAATGGGTC AGGATCCCTG GGATCCACGA CGTCAGTCAG CAGTGGAACA 
GCCACGGCCG CCACCAATGC TGCAGCTGAC CTGGGGTCCC CCAGCTTGCA GCACAGCAGA 
GATGACTCTT TAGATCTGAG CCCTCAAGGT CGATTAAGTG ATGTTCTAAA GAGAAAACGG 
CTGCCAAAAC GAGGGCCAAG AAGGCCAAAG TATTCACCTC CAAGAGATGA TGACAAAGTA 
GACAATCAAG CTAAAAGCCC TACCACTACT CAGTCACCTA AATCTTCTTT CCTGGCAAGC 
TTGAATCCAA AAACGTGGGG AAGGTTAAGC GCACAGTCCA ACAGCAACAA CATCGAACCA 
GCACGAACTG CGGGAGTCAG TGGTCTTGCC AGGGCCGCCT CCAAGGACAC CATATCCAAT 
AACAGAGAAA AAATTAAAGG TTGGATTAAG GAACAGGCGC ATAAATTTGT AGAGCGTTAT 
TTCAGTTCTG AGAACATGGA TGGAAGCAAC CCTGCACTGA ATGTCCTTCA GAGACTTTGT 
GCTGCAACTG AACAACTCAA CCTCCAGGTG GATGGTGGAG CTGAGTGCCT TGTAGAAATC 
CGTAGCATAG TCTCAGAGTC TGATGTTTCA TCATTTGAAA TCCAACATAG TGGATTTGTG 
AAGCAGCTGT TACTTTATTT GACATCTAAA AGTGAAAAGG ATGCTGTGAG CCGAGAGATC 
AGATTAAAGA GATTCCTTCA CGTATTTTTT TCTTCACCAC TTCCTGGAGA AGAGCCCATT 
GGAAGAGTAG AACCAGTGGG TAATGCGCCT TTGTTGGCAT TAGTTCACAA GATGAACAAC 
TGCCTCAGCC AGATGGAACA GTTTCCAGTC AAAGTACATG ACTTCCCCAG TGGGAACGGG 
ACAGGAGGCA GCTTTTCTCT CAACAGAGGA TCACAGGCTT TAAAATTTTT CAACACACAC 
CAGTTAAAAT GCCAGTTACA AAGACATCCA GACTGTGCAA ATGTGAAGCA GTGGAAGGGC 
GGGCCGGTCA AGATTGATCC CCTGGCTTTG GTACAAGCTA TTGAGAGATA CCTTGTAGTT 
AGAGGGTATG GAAGAGTAAG AGAAGATGAT GAAGACAGTG ATGATGATGG CTCTGATGAG 
GAAATAGACG AGTCTCTGGC CGCTCAGTTC TTAAATTCCG GAAACGTAAG GCACAGGCTA 
CAGTTTTATA TTGGAGAACA TTTGCTACCA TATAACATGA CTGTATATCA GGCAGTCCGG 
CAGTTCAGTA TACAAGCTGA GGATGAGCGG GAGTCCACAG ATGATGAGAG CAACCCTCTG 
GGAAGAGCTG GTATTTGGAC AAAGACTCAT ACAATATGGT ACAAACCTGT GAGAGAGGAT 
GAAGAAAGTA ATAAAGATTG TGTTGGTGGT AAAAGAGGAA GAGCCCAAAC GGCTCCAACA 
AAAACATCCC CTAGAAATGC AAAAAAGCAT GATGAGTTAT GGCATGATGG AGTATGCCCA 
TCAGTATCCA ATCCTTTAGA AGTTTACCTC ATCTCCTCGC CACCTGAGAA TATAACTTTT 
GAAGACCCGT CCTTAGATGT GATTCTTCTT CTAAGAGTTT TACATGCCAT CAGCCGATAC 
TGGTATTACT TGTATGATAA TGCAATGTGC AAGGAGATTA TTCCAACTAG TGAATTTATT 
AACAGTAAGT TGACAGCAAA AGCAAATAGG CAGCTTCAAG ATCCTTTAGT AATCATGACA 
GGAAATATCC CAACGTGGCT TACTGAACTA GGAAAAACCT GCCCATTTTT CTTCCCTTTT 
GATACCCGGC AAATGCTTTT TTACGTAACT GCATTTGATC GGGACCGAGC AATGCAAAGA 
TTACTTGATA CCAACCCAGA AATCAACCAG TCGGATTCTC AAGATAGCAG AGTTGCACCT 
AGATTGGATA GAAAAAAACG TACTGTGAAC AGAGAGGAGC TGCTGAAACA AGCGGAGTCT 
GTAATGCAGG ATCTCGGTAG CTCACGGGCC ATGTTAGAAA TCCAGTATGA AAATGAGGTT 
GGTACAGGTC TTGGGCCTAC ACTGGAGTTT TATGCACTTG TATCTCAGGA ACTTCAGAGA 
GCTGACTTGG GTCTCTGGAG AGGTGAAGAA GTGACTCTCA GCAATCCAAA AGGAAGCCAA 
GAAGGGACCA AGTATATTCA AAACCTCCAG GGCCTGTTTG CACTTCCCTT TGGTAGGACA 
GCTAAGCCAG CCCATATCGC AAAGGTTAAA ATGAAGTTTC GCTTCTTAGG AAAATTAATG 
GCCAAGGCTA TCATGGATTT CAGATTGGTG GACCTTCCCC TTGGTTTACC CTTTTATAAG 
TGGATGCTAA GGCAAGAAAC TTCACTGACG TCGCATGATT TGTTTGACAT TGACCCAGTT 
GTAGCCAGAT CAGTGTATCA CCTAGAAGAC ATTGTCAGAC AGAAGAAAAG ACTTGAGCAA 
GATAAATCCC AGACCAAAGA GAGTCTACAA TATGCATTAG AAACCCTGAC TATGAATGGC 
TGCTCAGTTG AAGATCTAGG ATTGGATTTC ACGCTGCCAG GGTTTCCCAA CATTGAACTG 
AAGAAAGGAG GAAAGGATAT ACCAGTTACC ATCCACAATT TAGAGGAGTA TCTAAGACTG 
GTTATATTCT GGGCACTAAA TGAAGGCGTT TCCAGGCAAT TTGATTCCTT CAGAGATGGA 
TTTGAATCTG TCTTCCCACT TAGTCATCTT CAGTACTTCT ACCCGGAAGA ACTGGATCAG 
CTCTTGTGCG GCAGTAAAGC AGACACGTGG GATGCGAAGA CACTGATGGA GTGCTGCAGG 
CCGGACCACG GTTATACTCA TGACAGTCGG GCTGTGAAGT TCTTGTTTGA GATTCTCAGT 
AGTTTTGATA ATGAGCAGCA GAGGTTGTTT CTCCAGTTTG TGACAGGTAG CCCAAGATTG 
CCTGTTGGAG GCTTCCGGAG TCTGAATCCA CCTCTGACAA TTGTCCGGAA GACCTTCGAG 
TCCACGGAGA ACCCCGACGA CTTCTTGCCC TCTGTAATGA CTTGTGTGAA CTACCTTAAG 
TTGCCGGACT ACTCGAGCAT TGACATCATG CGTGAGAAAC TGTTGATTGC AGCGAGAGAA 
GGGCAGCAGT CATTCCATCT TTCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TRIP12 ( XM_022409664.1 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_022265372.1 Canis lupus familiaris thyroid hormone receptor interactor 12 (TRIP12), transcript variant X4, mRNA.

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CloneID	OCe25762
Clone ID Related Accession (Same CDS sequence)	XM_022409664.1 , XM_005635802.2
Accession Version	XM_022409664.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6204bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-09-04
Organism	Canis lupus familiaris(dog)
Product	E3 ubiquitin-protein ligase TRIP12 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006607.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCCAACC GGCCTAATAA CAATCCAGGG GGGTCACTGC GACGTTCACA GAGGAACACT 
GCCGGGGCCC AACCACAAGA CGACTCGATA GGAGGAAGGT CACATTTAGG GCAGGCAAAA 
CATAAGGGAT ATAGCCCTCC TGAGAGTAGA AAATCTAATT CTAAGGCACC CAAAGTGCAG 
TCTAATACTA CTTCTGAACT GTCAAGGGGA CACCTTTCTA AAAGAAGCTG CAGTTCATCA 
TCTGCTGTCA TAGTTCCACA ACCAGAGGAT CCAGACAGAG CCAATACTTC AGAAAGGCAA 
AAAACGGGGC AGGTGCCTAA GAAAGACAAT TCTCGAGGAG TGAAACGCAG TGCTAGTCCA 
GACTACAACA GGACCAATTC TCCTAGCTCT GCCAAAAAAC CAAAAGCACT TCAGCATACT 
GAATCTCCCT CAGAAACAAA TAAGCCACAT AGTAAGTCGA AGAAGAGACA TTTAGACCAG 
GAGCAACAAC TGAAATCTGC ACAGTCGCCA TCAACAAGTA AGGCTCACAC CAGAAAGAGT 
GGGGCCACCG GCGGCTCACG GAGTCAGAAA AGAAAGAGGA CAGAGAGTTC TTGTCTAAAG 
AGTGGTTCTG TGTCTGAGTC AACTGGTGCC GAAGAGAGAT CTGCAAAACC CACCAAGCTG 
GCTTCAAAAT CCGCCGCCTC GGCCAAAGCT GGGTGCAGCA CCATCACTGA TTCTTCCTCC 
GCTGCCTCTA CTTCCTCCTC ATCTTCTGCT GTCGCCTCGG CCTCCTCCAC TGTACCACCA 
GGGGCCAGGG TGAAACAAGG AAAAGACCAG AGCAAGGCCA GGCGTTCCCG TTCAGCATCC 
AGTCCCAGTC CCAGAAGAAG TAGCAGGGAA AAGGAACAGA GTAAAACTGG TGGCTCTTCA 
AAATTCGATT GGGCTGCTCG TTTCAGCCCT AAAGTTAGCC TTCCTAAAAC CAAACTGTCT 
CTTCCAGGGT CTTCTAAGTC AGAGACATCA AAACCTGGAC CTTCTGGATT ACAGGCCAAA 
TTAGCAAGTT TAAGAAAATC TACCAAGAAG CGCAGTGAAT CCCCACCTGC TGAGCTCCCC 
AGTTTGAGGC GGAGCACACG CCAAAAGACC ACGGGCTCCT GTGCTAGTGC CAGTCGGCGA 
GGCTCTGGCC TGGGCAAAAG AGGAGCAGCT GAAGCTCGTC GACAGGAGAA GATGGCGGAC 
CCTGAAAGCA GCCAGGAGAC CGTGAATTCT TCAGCTGCAC GGACAGACGA AACTCCCCAA 
GGAGCTGCAG CCTCTAGTTC TGTTGCAGGG GCTGTGGGCA TGACCACCTC TGGGGAGAGT 
GAATCCGATG ATTCTGAGAT GGGACGACTA CAAGCTTTGT TAGAGGCCAG GGGTCTTCCT 
CCTCATCTGT TTGGCCCTCT CGGTCCTCGG ATGTCACAAC TTTTCCATAG AACAATTGGA 
AGCGGAGCTA GCTCTAAGGC CCAGCAGCTT CTGCAAGGAC TACAAGCAAG TGATGAAAGC 
CAGCAACTCC AGGCAGTTAT TGAAATGTGT CAGTTATTGG TTATGGGAAA TGAGGAGACG 
CTGGGAGGGT TTCCGGTCAA GAGTGTCGTT CCAGCTTTGA TAACATTACT GCAGATGGAG 
CATAATTTCG ATATTATGAA CCATGCTTGT CGAGCCTTAA CATATATGAT GGAAGCACTT 
CCTCGGTCAT CTGCTGTTGT AGTAGATGCA ATTCCTGTCT TTTTAGAAAA GCTGCAAGTT 
ATTCAGTGTA TTGATGTCGC AGAGCAGGCC CTGACTGCCT TGGAGATGTT ATCACGCAGA 
CACAGTAAAG CCATTCTACA GGCGGGTGGT TTGGCAGACT GCTTGCTGTA TCTAGAATTC 
TTCAGCATAA ATGCCCAAAG AAATGCACTA GCAATTGCAG CCAATTGCTG CCAGAGTATC 
ACGCCAGATG AATTTCATTT TGTGGCAGAT TCCCTCCCGT TGCTTACCCA AAGGCTAACC 
CATCAGGACA AAAAGTCAGT TGAAAGCACT TGCCTTTGTT TTGCACGTCT AGTGGACAAC 
TTCCAACATG AGGAGAATTT ACTCCAGCAG GTTGCCTCCA AAGATCTGCT TACGAATGTT 
CAACAACTCT TGGTAGTGAC TCCACCCATT TTAAGTTCTG GGATGTTTAT AATGGTGGTT 
CGCATGTTTT CTCTGATGTG TTCCAACTGT CCAACTTTAG CTGTTCAACT TATGAAGCAA 
AACATTGCAG AAACACTTCA CTTTCTCCTG TGTGGTGCCT CTAATGGAAG TTGTCAGGAA 
CAGATTGATC TTGTTCCACG AAGTCCACAA GAACTGTATG AACTGACATC TCTGATTTGC 
GAACTTATGC CATGTTTACC AAAAGAAGGC ATTTTTGCAG TTGATACCAT GCTGAAGAAG 
GGAAATGCGC AGAACACAGA CGGTGCGATT TGGCAGTGGC GTGATGACCG TGGCCTTTGG 
CATCCCTATA ACAGGATTGA CAGCCGGATC ATTGAGGCAG CCCATCAGGT CGGTGAGGAT 
GAGATAAGCT TGTCCACTCT GGGACGAGTT TATACTATTG ATTTTAATTC TATGCAGCAA 
ATCAATGAGG ACACGGGAAC AGCACGTGCC ATTCAGAGAA AACCTAACCC CTTAGCCAAT 
ACTAACACTA GTGGATATTC AGAGTTAAAG AAGGATGATG CTCGAGCACA GCTTATGAAA 
GAGGATCCGG AACTGGCTAA GTCTTTTATT AAGACATTAT TTGGTGTTCT TTATGAAGTG 
TATAGTTCCT CAGCAGGACC TGCGGTCAGA CATAAGTGCC TTAGAGCAAT TCTTAGGATA 
ATTTATTTTG CGGATGCTGA ACTTCTGAAG GATGTCCTGA AAAATCATGC TGTTTCAAGT 
CACATTGCTT CCATGCTGTC AAGCCAAGAC CTGAAGATAG TAGTTGGAGC ACTTCAGATG 
GCAGAAATCT TAATGCAGAA GTTACCTGAT ATTTTTAGTG TTTACTTCAG AAGAGAAGGT 
GTAATGCATC AAGTAAAACA CTTAGCAGAA TCAGAGTCTT TGTTGACAAG TCCACCAAAG 
GCATGTACGA ATGGGTCAGG ATCCCTGGGA TCCACGACGT CAGTCAGCAG TGGAACAGCC 
ACGGCCGCCA CCAATGCTGC AGCTGACCTG GGGTCCCCCA GCTTGCAGCA CAGCAGAGAT 
GACTCTTTAG ATCTGAGCCC TCAAGGTCGA TTAAGTGATG TTCTAAAGAG AAAACGGCTG 
CCAAAACGAG GGCCAAGAAG GCCAAAGTAT TCACCTCCAA GAGATGATGA CAAAGTAGAC 
AATCAAGCTA AAAGCCCTAC CACTACTCAG TCACCTAAAT CTTCTTTCCT GGCAAGCTTG 
AATCCAAAAA CGTGGGGAAG GTTAAGCGCA CAGTCCAACA GCAACAACAT CGAACCAGCA 
CGAACTGCGG GAGTCAGTGG TCTTGCCAGG GCCGCCTCCA AGGACACCAT ATCCAATAAC 
AGAGAAAAAA TTAAAGGTTG GATTAAGGAA CAGGCGCATA AATTTGTAGA GCGTTATTTC 
AGTTCTGAGA ACATGGATGG AAGCAACCCT GCACTGAATG TCCTTCAGAG ACTTTGTGCT 
GCAACTGAAC AACTCAACCT CCAGGTGGAT GGTGGAGCTG AGTGCCTTGT AGAAATCCGT 
AGCATAGTCT CAGAGTCTGA TGTTTCATCA TTTGAAATCC AACATAGTGG ATTTGTGAAG 
CAGCTGTTAC TTTATTTGAC ATCTAAAAGT GAAAAGGATG CTGTGAGCCG AGAGATCAGA 
TTAAAGAGAT TCCTTCACGT ATTTTTTTCT TCACCACTTC CTGGAGAAGA GCCCATTGGA 
AGAGTAGAAC CAGTGGGTAA TGCGCCTTTG TTGGCATTAG TTCACAAGAT GAACAACTGC 
CTCAGCCAGA TGGAACAGTT TCCAGTCAAA GTACATGACT TCCCCAGTGG GAACGGGACA 
GGAGGCAGCT TTTCTCTCAA CAGAGGATCA CAGGCTTTAA AATTTTTCAA CACACACCAG 
TTAAAATGCC AGTTACAAAG ACATCCAGAC TGTGCAAATG TGAAGCAGTG GAAGGGCGGG 
CCGGTCAAGA TTGATCCCCT GGCTTTGGTA CAAGCTATTG AGAGATACCT TGTAGTTAGA 
GGGTATGGAA GAGTAAGAGA AGATGATGAA GACAGTGATG ATGATGGCTC TGATGAGGAA 
ATAGACGAGT CTCTGGCCGC TCAGTTCTTA AATTCCGGAA ACGTAAGGCA CAGGCTACAG 
TTTTATATTG GAGAACATTT GCTACCATAT AACATGACTG TATATCAGGC AGTCCGGCAG 
TTCAGTATAC AAGCTGAGGA TGAGCGGGAG TCCACAGATG ATGAGAGCAA CCCTCTGGGA 
AGAGCTGGTA TTTGGACAAA GACTCATACA ATATGGTACA AACCTGTGAG AGAGGATGAA 
GAAAGTAATA AAGATTGTGT TGGTGGTAAA AGAGGAAGAG CCCAAACGGC TCCAACAAAA 
ACATCCCCTA GAAATGCAAA AAAGCATGAT GAGTTATGGC ATGATGGAGT ATGCCCATCA 
GTATCCAATC CTTTAGAAGT TTACCTCATC TCCTCGCCAC CTGAGAATAT AACTTTTGAA 
GACCCGTCCT TAGATGTGAT TCTTCTTCTA AGAGTTTTAC ATGCCATCAG CCGATACTGG 
TATTACTTGT ATGATAATGC AATGTGCAAG GAGATTATTC CAACTAGTGA ATTTATTAAC 
AGTAAGTTGA CAGCAAAAGC AAATAGGCAG CTTCAAGATC CTTTAGTAAT CATGACAGGA 
AATATCCCAA CGTGGCTTAC TGAACTAGGA AAAACCTGCC CATTTTTCTT CCCTTTTGAT 
ACCCGGCAAA TGCTTTTTTA CGTAACTGCA TTTGATCGGG ACCGAGCAAT GCAAAGATTA 
CTTGATACCA ACCCAGAAAT CAACCAGTCG GATTCTCAAG ATAGCAGAGT TGCACCTAGA 
TTGGATAGAA AAAAACGTAC TGTGAACAGA GAGGAGCTGC TGAAACAAGC GGAGTCTGTA 
ATGCAGGATC TCGGTAGCTC ACGGGCCATG TTAGAAATCC AGTATGAAAA TGAGGTTGGT 
ACAGGTCTTG GGCCTACACT GGAGTTTTAT GCACTTGTAT CTCAGGAACT TCAGAGAGCT 
GACTTGGGTC TCTGGAGAGG TGAAGAAGTG ACTCTCAGCA ATCCAAAAGG AAGCCAAGAA 
GGGACCAAGT ATATTCAAAA CCTCCAGGGC CTGTTTGCAC TTCCCTTTGG TAGGACAGCT 
AAGCCAGCCC ATATCGCAAA GGTTAAAATG AAGTTTCGCT TCTTAGGAAA ATTAATGGCC 
AAGGCTATCA TGGATTTCAG ATTGGTGGAC CTTCCCCTTG GTTTACCCTT TTATAAGTGG 
ATGCTAAGGC AAGAAACTTC ACTGACGTCG CATGATTTGT TTGACATTGA CCCAGTTGTA 
GCCAGATCAG TGTATCACCT AGAAGACATT GTCAGACAGA AGAAAAGACT TGAGCAAGAT 
AAATCCCAGA CCAAAGAGAG TCTACAATAT GCATTAGAAA CCCTGACTAT GAATGGCTGC 
TCAGTTGAAG ATCTAGGATT GGATTTCACG CTGCCAGGGT TTCCCAACAT TGAACTGAAG 
AAAGGAGGAA AGGATATACC AGTTACCATC CACAATTTAG AGGAGTATCT AAGACTGGTT 
ATATTCTGGG CACTAAATGA AGGCGTTTCC AGGCAATTTG ATTCCTTCAG AGATGGATTT 
GAATCTGTCT TCCCACTTAG TCATCTTCAG TACTTCTACC CGGAAGAACT GGATCAGCTC 
TTGTGCGGCA GTAAAGCAGA CACGTGGGAT GCGAAGACAC TGATGGAGTG CTGCAGGCCG 
GACCACGGTT ATACTCATGA CAGTCGGGCT GTGAAGTTCT TGTTTGAGAT TCTCAGTAGT 
TTTGATAATG AGCAGCAGAG GTTGTTTCTC CAGTTTGTGA CAGGTAGCCC AAGATTGCCT 
GTTGGAGGCT TCCGGAGTCT GAATCCACCT CTGACAATTG TCCGGAAGAC CTTCGAGTCC 
ACGGAGAACC CCGACGACTT CTTGCCCTCT GTAATGACTT GTGTGAACTA CCTTAAGTTG 
CCGGACTACT CGAGCATTGA CATCATGCGT GAGAAACTGT TGATTGCAGC GAGAGAAGGG 
CAGCAGTCAT TCCATCTTTC CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_022265372.1
CDS	1..6204
Translation	MSNRPNNNPGGSLRRSQRNTAGAQPQDDSIGGRSHLGQAKHKGYSPPESRKSNSKAPKVQSNTTSELSRGHLSKRSCSSSSAVIVPQPEDPDRANTSERQKTGQVPKKDNSRGVKRSASPDYNRTNSPSSAKKPKALQHTESPSETNKPHSKSKKRHLDQEQQLKSAQSPSTSKAHTRKSGATGGSRSQKRKRTESSCLKSGSVSESTGAEERSAKPTKLASKSAASAKAGCSTITDSSSAASTSSSSSAVASASSTVPPGARVKQGKDQSKARRSRSASSPSPRRSSREKEQSKTGGSSKFDWAARFSPKVSLPKTKLSLPGSSKSETSKPGPSGLQAKLASLRKSTKKRSESPPAELPSLRRSTRQKTTGSCASASRRGSGLGKRGAAEARRQEKMADPESSQETVNSSAARTDETPQGAAASSSVAGAVGMTTSGESESDDSEMGRLQALLEARGLPPHLFGPLGPRMSQLFHRTIGSGASSKAQQLLQGLQASDESQQLQAVIEMCQLLVMGNEETLGGFPVKSVVPALITLLQMEHNFDIMNHACRALTYMMEALPRSSAVVVDAIPVFLEKLQVIQCIDVAEQALTALEMLSRRHSKAILQAGGLADCLLYLEFFSINAQRNALAIAANCCQSITPDEFHFVADSLPLLTQRLTHQDKKSVESTCLCFARLVDNFQHEENLLQQVASKDLLTNVQQLLVVTPPILSSGMFIMVVRMFSLMCSNCPTLAVQLMKQNIAETLHFLLCGASNGSCQEQIDLVPRSPQELYELTSLICELMPCLPKEGIFAVDTMLKKGNAQNTDGAIWQWRDDRGLWHPYNRIDSRIIEAAHQVGEDEISLSTLGRVYTIDFNSMQQINEDTGTARAIQRKPNPLANTNTSGYSELKKDDARAQLMKEDPELAKSFIKTLFGVLYEVYSSSAGPAVRHKCLRAILRIIYFADAELLKDVLKNHAVSSHIASMLSSQDLKIVVGALQMAEILMQKLPDIFSVYFRREGVMHQVKHLAESESLLTSPPKACTNGSGSLGSTTSVSSGTATAATNAAADLGSPSLQHSRDDSLDLSPQGRLSDVLKRKRLPKRGPRRPKYSPPRDDDKVDNQAKSPTTTQSPKSSFLASLNPKTWGRLSAQSNSNNIEPARTAGVSGLARAASKDTISNNREKIKGWIKEQAHKFVERYFSSENMDGSNPALNVLQRLCAATEQLNLQVDGGAECLVEIRSIVSESDVSSFEIQHSGFVKQLLLYLTSKSEKDAVSREIRLKRFLHVFFSSPLPGEEPIGRVEPVGNAPLLALVHKMNNCLSQMEQFPVKVHDFPSGNGTGGSFSLNRGSQALKFFNTHQLKCQLQRHPDCANVKQWKGGPVKIDPLALVQAIERYLVVRGYGRVREDDEDSDDDGSDEEIDESLAAQFLNSGNVRHRLQFYIGEHLLPYNMTVYQAVRQFSIQAEDERESTDDESNPLGRAGIWTKTHTIWYKPVREDEESNKDCVGGKRGRAQTAPTKTSPRNAKKHDELWHDGVCPSVSNPLEVYLISSPPENITFEDPSLDVILLLRVLHAISRYWYYLYDNAMCKEIIPTSEFINSKLTAKANRQLQDPLVIMTGNIPTWLTELGKTCPFFFPFDTRQMLFYVTAFDRDRAMQRLLDTNPEINQSDSQDSRVAPRLDRKKRTVNREELLKQAESVMQDLGSSRAMLEIQYENEVGTGLGPTLEFYALVSQELQRADLGLWRGEEVTLSNPKGSQEGTKYIQNLQGLFALPFGRTAKPAHIAKVKMKFRFLGKLMAKAIMDFRLVDLPLGLPFYKWMLRQETSLTSHDLFDIDPVVARSVYHLEDIVRQKKRLEQDKSQTKESLQYALETLTMNGCSVEDLGLDFTLPGFPNIELKKGGKDIPVTIHNLEEYLRLVIFWALNEGVSRQFDSFRDGFESVFPLSHLQYFYPEELDQLLCGSKADTWDAKTLMECCRPDHGYTHDSRAVKFLFEILSSFDNEQQRLFLQFVTGSPRLPVGGFRSLNPPLTIVRKTFESTENPDDFLPSVMTCVNYLKLPDYSSIDIMREKLLIAAREGQQSFHLS

Target ORF information:

RefSeq Version	XM_022409664.1
Organism	Canis lupus familiaris(dog)
Definition	Canis lupus familiaris thyroid hormone receptor interactor 12 (TRIP12), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_022409664.1

ORF Insert Sequence:

ATGTCCAACC GGCCTAATAA CAATCCAGGG GGGTCACTGC GACGTTCACA GAGGAACACT 
GCCGGGGCCC AACCACAAGA CGACTCGATA GGAGGAAGGT CACATTTAGG GCAGGCAAAA 
CATAAGGGAT ATAGCCCTCC TGAGAGTAGA AAATCTAATT CTAAGGCACC CAAAGTGCAG 
TCTAATACTA CTTCTGAACT GTCAAGGGGA CACCTTTCTA AAAGAAGCTG CAGTTCATCA 
TCTGCTGTCA TAGTTCCACA ACCAGAGGAT CCAGACAGAG CCAATACTTC AGAAAGGCAA 
AAAACGGGGC AGGTGCCTAA GAAAGACAAT TCTCGAGGAG TGAAACGCAG TGCTAGTCCA 
GACTACAACA GGACCAATTC TCCTAGCTCT GCCAAAAAAC CAAAAGCACT TCAGCATACT 
GAATCTCCCT CAGAAACAAA TAAGCCACAT AGTAAGTCGA AGAAGAGACA TTTAGACCAG 
GAGCAACAAC TGAAATCTGC ACAGTCGCCA TCAACAAGTA AGGCTCACAC CAGAAAGAGT 
GGGGCCACCG GCGGCTCACG GAGTCAGAAA AGAAAGAGGA CAGAGAGTTC TTGTCTAAAG 
AGTGGTTCTG TGTCTGAGTC AACTGGTGCC GAAGAGAGAT CTGCAAAACC CACCAAGCTG 
GCTTCAAAAT CCGCCGCCTC GGCCAAAGCT GGGTGCAGCA CCATCACTGA TTCTTCCTCC 
GCTGCCTCTA CTTCCTCCTC ATCTTCTGCT GTCGCCTCGG CCTCCTCCAC TGTACCACCA 
GGGGCCAGGG TGAAACAAGG AAAAGACCAG AGCAAGGCCA GGCGTTCCCG TTCAGCATCC 
AGTCCCAGTC CCAGAAGAAG TAGCAGGGAA AAGGAACAGA GTAAAACTGG TGGCTCTTCA 
AAATTCGATT GGGCTGCTCG TTTCAGCCCT AAAGTTAGCC TTCCTAAAAC CAAACTGTCT 
CTTCCAGGGT CTTCTAAGTC AGAGACATCA AAACCTGGAC CTTCTGGATT ACAGGCCAAA 
TTAGCAAGTT TAAGAAAATC TACCAAGAAG CGCAGTGAAT CCCCACCTGC TGAGCTCCCC 
AGTTTGAGGC GGAGCACACG CCAAAAGACC ACGGGCTCCT GTGCTAGTGC CAGTCGGCGA 
GGCTCTGGCC TGGGCAAAAG AGGAGCAGCT GAAGCTCGTC GACAGGAGAA GATGGCGGAC 
CCTGAAAGCA GCCAGGAGAC CGTGAATTCT TCAGCTGCAC GGACAGACGA AACTCCCCAA 
GGAGCTGCAG CCTCTAGTTC TGTTGCAGGG GCTGTGGGCA TGACCACCTC TGGGGAGAGT 
GAATCCGATG ATTCTGAGAT GGGACGACTA CAAGCTTTGT TAGAGGCCAG GGGTCTTCCT 
CCTCATCTGT TTGGCCCTCT CGGTCCTCGG ATGTCACAAC TTTTCCATAG AACAATTGGA 
AGCGGAGCTA GCTCTAAGGC CCAGCAGCTT CTGCAAGGAC TACAAGCAAG TGATGAAAGC 
CAGCAACTCC AGGCAGTTAT TGAAATGTGT CAGTTATTGG TTATGGGAAA TGAGGAGACG 
CTGGGAGGGT TTCCGGTCAA GAGTGTCGTT CCAGCTTTGA TAACATTACT GCAGATGGAG 
CATAATTTCG ATATTATGAA CCATGCTTGT CGAGCCTTAA CATATATGAT GGAAGCACTT 
CCTCGGTCAT CTGCTGTTGT AGTAGATGCA ATTCCTGTCT TTTTAGAAAA GCTGCAAGTT 
ATTCAGTGTA TTGATGTCGC AGAGCAGGCC CTGACTGCCT TGGAGATGTT ATCACGCAGA 
CACAGTAAAG CCATTCTACA GGCGGGTGGT TTGGCAGACT GCTTGCTGTA TCTAGAATTC 
TTCAGCATAA ATGCCCAAAG AAATGCACTA GCAATTGCAG CCAATTGCTG CCAGAGTATC 
ACGCCAGATG AATTTCATTT TGTGGCAGAT TCCCTCCCGT TGCTTACCCA AAGGCTAACC 
CATCAGGACA AAAAGTCAGT TGAAAGCACT TGCCTTTGTT TTGCACGTCT AGTGGACAAC 
TTCCAACATG AGGAGAATTT ACTCCAGCAG GTTGCCTCCA AAGATCTGCT TACGAATGTT 
CAACAACTCT TGGTAGTGAC TCCACCCATT TTAAGTTCTG GGATGTTTAT AATGGTGGTT 
CGCATGTTTT CTCTGATGTG TTCCAACTGT CCAACTTTAG CTGTTCAACT TATGAAGCAA 
AACATTGCAG AAACACTTCA CTTTCTCCTG TGTGGTGCCT CTAATGGAAG TTGTCAGGAA 
CAGATTGATC TTGTTCCACG AAGTCCACAA GAACTGTATG AACTGACATC TCTGATTTGC 
GAACTTATGC CATGTTTACC AAAAGAAGGC ATTTTTGCAG TTGATACCAT GCTGAAGAAG 
GGAAATGCGC AGAACACAGA CGGTGCGATT TGGCAGTGGC GTGATGACCG TGGCCTTTGG 
CATCCCTATA ACAGGATTGA CAGCCGGATC ATTGAGGCAG CCCATCAGGT CGGTGAGGAT 
GAGATAAGCT TGTCCACTCT GGGACGAGTT TATACTATTG ATTTTAATTC TATGCAGCAA 
ATCAATGAGG ACACGGGAAC AGCACGTGCC ATTCAGAGAA AACCTAACCC CTTAGCCAAT 
ACTAACACTA GTGGATATTC AGAGTTAAAG AAGGATGATG CTCGAGCACA GCTTATGAAA 
GAGGATCCGG AACTGGCTAA GTCTTTTATT AAGACATTAT TTGGTGTTCT TTATGAAGTG 
TATAGTTCCT CAGCAGGACC TGCGGTCAGA CATAAGTGCC TTAGAGCAAT TCTTAGGATA 
ATTTATTTTG CGGATGCTGA ACTTCTGAAG GATGTCCTGA AAAATCATGC TGTTTCAAGT 
CACATTGCTT CCATGCTGTC AAGCCAAGAC CTGAAGATAG TAGTTGGAGC ACTTCAGATG 
GCAGAAATCT TAATGCAGAA GTTACCTGAT ATTTTTAGTG TTTACTTCAG AAGAGAAGGT 
GTAATGCATC AAGTAAAACA CTTAGCAGAA TCAGAGTCTT TGTTGACAAG TCCACCAAAG 
GCATGTACGA ATGGGTCAGG ATCCCTGGGA TCCACGACGT CAGTCAGCAG TGGAACAGCC 
ACGGCCGCCA CCAATGCTGC AGCTGACCTG GGGTCCCCCA GCTTGCAGCA CAGCAGAGAT 
GACTCTTTAG ATCTGAGCCC TCAAGGTCGA TTAAGTGATG TTCTAAAGAG AAAACGGCTG 
CCAAAACGAG GGCCAAGAAG GCCAAAGTAT TCACCTCCAA GAGATGATGA CAAAGTAGAC 
AATCAAGCTA AAAGCCCTAC CACTACTCAG TCACCTAAAT CTTCTTTCCT GGCAAGCTTG 
AATCCAAAAA CGTGGGGAAG GTTAAGCGCA CAGTCCAACA GCAACAACAT CGAACCAGCA 
CGAACTGCGG GAGTCAGTGG TCTTGCCAGG GCCGCCTCCA AGGACACCAT ATCCAATAAC 
AGAGAAAAAA TTAAAGGTTG GATTAAGGAA CAGGCGCATA AATTTGTAGA GCGTTATTTC 
AGTTCTGAGA ACATGGATGG AAGCAACCCT GCACTGAATG TCCTTCAGAG ACTTTGTGCT 
GCAACTGAAC AACTCAACCT CCAGGTGGAT GGTGGAGCTG AGTGCCTTGT AGAAATCCGT 
AGCATAGTCT CAGAGTCTGA TGTTTCATCA TTTGAAATCC AACATAGTGG ATTTGTGAAG 
CAGCTGTTAC TTTATTTGAC ATCTAAAAGT GAAAAGGATG CTGTGAGCCG AGAGATCAGA 
TTAAAGAGAT TCCTTCACGT ATTTTTTTCT TCACCACTTC CTGGAGAAGA GCCCATTGGA 
AGAGTAGAAC CAGTGGGTAA TGCGCCTTTG TTGGCATTAG TTCACAAGAT GAACAACTGC 
CTCAGCCAGA TGGAACAGTT TCCAGTCAAA GTACATGACT TCCCCAGTGG GAACGGGACA 
GGAGGCAGCT TTTCTCTCAA CAGAGGATCA CAGGCTTTAA AATTTTTCAA CACACACCAG 
TTAAAATGCC AGTTACAAAG ACATCCAGAC TGTGCAAATG TGAAGCAGTG GAAGGGCGGG 
CCGGTCAAGA TTGATCCCCT GGCTTTGGTA CAAGCTATTG AGAGATACCT TGTAGTTAGA 
GGGTATGGAA GAGTAAGAGA AGATGATGAA GACAGTGATG ATGATGGCTC TGATGAGGAA 
ATAGACGAGT CTCTGGCCGC TCAGTTCTTA AATTCCGGAA ACGTAAGGCA CAGGCTACAG 
TTTTATATTG GAGAACATTT GCTACCATAT AACATGACTG TATATCAGGC AGTCCGGCAG 
TTCAGTATAC AAGCTGAGGA TGAGCGGGAG TCCACAGATG ATGAGAGCAA CCCTCTGGGA 
AGAGCTGGTA TTTGGACAAA GACTCATACA ATATGGTACA AACCTGTGAG AGAGGATGAA 
GAAAGTAATA AAGATTGTGT TGGTGGTAAA AGAGGAAGAG CCCAAACGGC TCCAACAAAA 
ACATCCCCTA GAAATGCAAA AAAGCATGAT GAGTTATGGC ATGATGGAGT ATGCCCATCA 
GTATCCAATC CTTTAGAAGT TTACCTCATC TCCTCGCCAC CTGAGAATAT AACTTTTGAA 
GACCCGTCCT TAGATGTGAT TCTTCTTCTA AGAGTTTTAC ATGCCATCAG CCGATACTGG 
TATTACTTGT ATGATAATGC AATGTGCAAG GAGATTATTC CAACTAGTGA ATTTATTAAC 
AGTAAGTTGA CAGCAAAAGC AAATAGGCAG CTTCAAGATC CTTTAGTAAT CATGACAGGA 
AATATCCCAA CGTGGCTTAC TGAACTAGGA AAAACCTGCC CATTTTTCTT CCCTTTTGAT 
ACCCGGCAAA TGCTTTTTTA CGTAACTGCA TTTGATCGGG ACCGAGCAAT GCAAAGATTA 
CTTGATACCA ACCCAGAAAT CAACCAGTCG GATTCTCAAG ATAGCAGAGT TGCACCTAGA 
TTGGATAGAA AAAAACGTAC TGTGAACAGA GAGGAGCTGC TGAAACAAGC GGAGTCTGTA 
ATGCAGGATC TCGGTAGCTC ACGGGCCATG TTAGAAATCC AGTATGAAAA TGAGGTTGGT 
ACAGGTCTTG GGCCTACACT GGAGTTTTAT GCACTTGTAT CTCAGGAACT TCAGAGAGCT 
GACTTGGGTC TCTGGAGAGG TGAAGAAGTG ACTCTCAGCA ATCCAAAAGG AAGCCAAGAA 
GGGACCAAGT ATATTCAAAA CCTCCAGGGC CTGTTTGCAC TTCCCTTTGG TAGGACAGCT 
AAGCCAGCCC ATATCGCAAA GGTTAAAATG AAGTTTCGCT TCTTAGGAAA ATTAATGGCC 
AAGGCTATCA TGGATTTCAG ATTGGTGGAC CTTCCCCTTG GTTTACCCTT TTATAAGTGG 
ATGCTAAGGC AAGAAACTTC ACTGACGTCG CATGATTTGT TTGACATTGA CCCAGTTGTA 
GCCAGATCAG TGTATCACCT AGAAGACATT GTCAGACAGA AGAAAAGACT TGAGCAAGAT 
AAATCCCAGA CCAAAGAGAG TCTACAATAT GCATTAGAAA CCCTGACTAT GAATGGCTGC 
TCAGTTGAAG ATCTAGGATT GGATTTCACG CTGCCAGGGT TTCCCAACAT TGAACTGAAG 
AAAGGAGGAA AGGATATACC AGTTACCATC CACAATTTAG AGGAGTATCT AAGACTGGTT 
ATATTCTGGG CACTAAATGA AGGCGTTTCC AGGCAATTTG ATTCCTTCAG AGATGGATTT 
GAATCTGTCT TCCCACTTAG TCATCTTCAG TACTTCTACC CGGAAGAACT GGATCAGCTC 
TTGTGCGGCA GTAAAGCAGA CACGTGGGAT GCGAAGACAC TGATGGAGTG CTGCAGGCCG 
GACCACGGTT ATACTCATGA CAGTCGGGCT GTGAAGTTCT TGTTTGAGAT TCTCAGTAGT 
TTTGATAATG AGCAGCAGAG GTTGTTTCTC CAGTTTGTGA CAGGTAGCCC AAGATTGCCT 
GTTGGAGGCT TCCGGAGTCT GAATCCACCT CTGACAATTG TCCGGAAGAC CTTCGAGTCC 
ACGGAGAACC CCGACGACTT CTTGCCCTCT GTAATGACTT GTGTGAACTA CCTTAAGTTG 
CCGGACTACT CGAGCATTGA CATCATGCGT GAGAAACTGT TGATTGCAGC GAGAGAAGGG 
CAGCAGTCAT TCCATCTTTC CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TRIP12 ( XM_005635802.2 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_005635859.1 Canis lupus familiaris thyroid hormone receptor interactor 12 (TRIP12), transcript variant X3, mRNA.

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CloneID	OCe25762
Clone ID Related Accession (Same CDS sequence)	XM_022409664.1 , XM_005635802.2
Accession Version	XM_005635802.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6204bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-09-16
Organism	Canis lupus familiaris(Dog)
Product	E3 ubiquitin-protein ligase TRIP12 isoform X2
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726103.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 17, 2015 this sequence version replaced XM_005635802.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCCAACC GGCCTAATAA CAATCCAGGG GGGTCACTGC GACGTTCACA GAGGAACACT 
GCCGGGGCCC AACCACAAGA CGACTCGATA GGAGGAAGGT CACATTTAGG GCAGGCAAAA 
CATAAGGGAT ATAGCCCTCC TGAGAGTAGA AAATCTAATT CTAAGGCACC CAAAGTGCAG 
TCTAATACTA CTTCTGAACT GTCAAGGGGA CACCTTTCTA AAAGAAGCTG CAGTTCATCA 
TCTGCTGTCA TAGTTCCACA ACCAGAGGAT CCAGACAGAG CCAATACTTC AGAAAGGCAA 
AAAACGGGGC AGGTGCCTAA GAAAGACAAT TCTCGAGGAG TGAAACGCAG TGCTAGTCCA 
GACTACAACA GGACCAATTC TCCTAGCTCT GCCAAAAAAC CAAAAGCACT TCAGCATACT 
GAATCTCCCT CAGAAACAAA TAAGCCACAT AGTAAGTCGA AGAAGAGACA TTTAGACCAG 
GAGCAACAAC TGAAATCTGC ACAGTCGCCA TCAACAAGTA AGGCTCACAC CAGAAAGAGT 
GGGGCCACCG GCGGCTCACG GAGTCAGAAA AGAAAGAGGA CAGAGAGTTC TTGTCTAAAG 
AGTGGTTCTG TGTCTGAGTC AACTGGTGCC GAAGAGAGAT CTGCAAAACC CACCAAGCTG 
GCTTCAAAAT CCGCCGCCTC GGCCAAAGCT GGGTGCAGCA CCATCACTGA TTCTTCCTCC 
GCTGCCTCTA CTTCCTCCTC ATCTTCTGCT GTCGCCTCGG CCTCCTCCAC TGTACCACCA 
GGGGCCAGGG TGAAACAAGG AAAAGACCAG AGCAAGGCCA GGCGTTCCCG TTCAGCATCC 
AGTCCCAGTC CCAGAAGAAG TAGCAGGGAA AAGGAACAGA GTAAAACTGG TGGCTCTTCA 
AAATTCGATT GGGCTGCTCG TTTCAGCCCT AAAGTTAGCC TTCCTAAAAC CAAACTGTCT 
CTTCCAGGGT CTTCTAAGTC AGAGACATCA AAACCTGGAC CTTCTGGATT ACAGGCCAAA 
TTAGCAAGTT TAAGAAAATC TACCAAGAAG CGCAGTGAAT CCCCACCTGC TGAGCTCCCC 
AGTTTGAGGC GGAGCACACG CCAAAAGACC ACGGGCTCCT GTGCTAGTGC CAGTCGGCGA 
GGCTCTGGCC TGGGCAAAAG AGGAGCAGCT GAAGCTCGTC GACAGGAGAA GATGGCGGAC 
CCTGAAAGCA GCCAGGAGAC CGTGAATTCT TCAGCTGCAC GGACAGACGA AACTCCCCAA 
GGAGCTGCAG CCTCTAGTTC TGTTGCAGGG GCTGTGGGCA TGACCACCTC TGGGGAGAGT 
GAATCCGATG ATTCTGAGAT GGGACGACTA CAAGCTTTGT TAGAGGCCAG GGGTCTTCCT 
CCTCATCTGT TTGGCCCTCT CGGTCCTCGG ATGTCACAAC TTTTCCATAG AACAATTGGA 
AGCGGAGCTA GCTCTAAGGC CCAGCAGCTT CTGCAAGGAC TACAAGCAAG TGATGAAAGC 
CAGCAACTCC AGGCAGTTAT TGAAATGTGT CAGTTATTGG TTATGGGAAA TGAGGAGACG 
CTGGGAGGGT TTCCGGTCAA GAGTGTCGTT CCAGCTTTGA TAACATTACT GCAGATGGAG 
CATAATTTCG ATATTATGAA CCATGCTTGT CGAGCCTTAA CATATATGAT GGAAGCACTT 
CCTCGGTCAT CTGCTGTTGT AGTAGATGCA ATTCCTGTCT TTTTAGAAAA GCTGCAAGTT 
ATTCAGTGTA TTGATGTCGC AGAGCAGGCC CTGACTGCCT TGGAGATGTT ATCACGCAGA 
CACAGTAAAG CCATTCTACA GGCGGGTGGT TTGGCAGACT GCTTGCTGTA TCTAGAATTC 
TTCAGCATAA ATGCCCAAAG AAATGCACTA GCAATTGCAG CCAATTGCTG CCAGAGTATC 
ACGCCAGATG AATTTCATTT TGTGGCAGAT TCCCTCCCGT TGCTTACCCA AAGGCTAACC 
CATCAGGACA AAAAGTCAGT TGAAAGCACT TGCCTTTGTT TTGCACGTCT AGTGGACAAC 
TTCCAACATG AGGAGAATTT ACTCCAGCAG GTTGCCTCCA AAGATCTGCT TACGAATGTT 
CAACAACTCT TGGTAGTGAC TCCACCCATT TTAAGTTCTG GGATGTTTAT AATGGTGGTT 
CGCATGTTTT CTCTGATGTG TTCCAACTGT CCAACTTTAG CTGTTCAACT TATGAAGCAA 
AACATTGCAG AAACACTTCA CTTTCTCCTG TGTGGTGCCT CTAATGGAAG TTGTCAGGAA 
CAGATTGATC TTGTTCCACG AAGTCCACAA GAACTGTATG AACTGACATC TCTGATTTGC 
GAACTTATGC CATGTTTACC AAAAGAAGGC ATTTTTGCAG TTGATACCAT GCTGAAGAAG 
GGAAATGCGC AGAACACAGA CGGTGCGATT TGGCAGTGGC GTGATGACCG TGGCCTTTGG 
CATCCCTATA ACAGGATTGA CAGCCGGATC ATTGAGGCAG CCCATCAGGT CGGTGAGGAT 
GAGATAAGCT TGTCCACTCT GGGACGAGTT TATACTATTG ATTTTAATTC TATGCAGCAA 
ATCAATGAGG ACACGGGAAC AGCACGTGCC ATTCAGAGAA AACCTAACCC CTTAGCCAAT 
ACTAACACTA GTGGATATTC AGAGTTAAAG AAGGATGATG CTCGAGCACA GCTTATGAAA 
GAGGATCCGG AACTGGCTAA GTCTTTTATT AAGACATTAT TTGGTGTTCT TTATGAAGTG 
TATAGTTCCT CAGCAGGACC TGCGGTCAGA CATAAGTGCC TTAGAGCAAT TCTTAGGATA 
ATTTATTTTG CGGATGCTGA ACTTCTGAAG GATGTCCTGA AAAATCATGC TGTTTCAAGT 
CACATTGCTT CCATGCTGTC AAGCCAAGAC CTGAAGATAG TAGTTGGAGC ACTTCAGATG 
GCAGAAATCT TAATGCAGAA GTTACCTGAT ATTTTTAGTG TTTACTTCAG AAGAGAAGGT 
GTAATGCATC AAGTAAAACA CTTAGCAGAA TCAGAGTCTT TGTTGACAAG TCCACCAAAG 
GCATGTACGA ATGGGTCAGG ATCCCTGGGA TCCACGACGT CAGTCAGCAG TGGAACAGCC 
ACGGCCGCCA CCAATGCTGC AGCTGACCTG GGGTCCCCCA GCTTGCAGCA CAGCAGAGAT 
GACTCTTTAG ATCTGAGCCC TCAAGGTCGA TTAAGTGATG TTCTAAAGAG AAAACGGCTG 
CCAAAACGAG GGCCAAGAAG GCCAAAGTAT TCACCTCCAA GAGATGATGA CAAAGTAGAC 
AATCAAGCTA AAAGCCCTAC CACTACTCAG TCACCTAAAT CTTCTTTCCT GGCAAGCTTG 
AATCCAAAAA CGTGGGGAAG GTTAAGCGCA CAGTCCAACA GCAACAACAT CGAACCAGCA 
CGAACTGCGG GAGTCAGTGG TCTTGCCAGG GCCGCCTCCA AGGACACCAT ATCCAATAAC 
AGAGAAAAAA TTAAAGGTTG GATTAAGGAA CAGGCGCATA AATTTGTAGA GCGTTATTTC 
AGTTCTGAGA ACATGGATGG AAGCAACCCT GCACTGAATG TCCTTCAGAG ACTTTGTGCT 
GCAACTGAAC AACTCAACCT CCAGGTGGAT GGTGGAGCTG AGTGCCTTGT AGAAATCCGT 
AGCATAGTCT CAGAGTCTGA TGTTTCATCA TTTGAAATCC AACATAGTGG ATTTGTGAAG 
CAGCTGTTAC TTTATTTGAC ATCTAAAAGT GAAAAGGATG CTGTGAGCCG AGAGATCAGA 
TTAAAGAGAT TCCTTCACGT ATTTTTTTCT TCACCACTTC CTGGAGAAGA GCCCATTGGA 
AGAGTAGAAC CAGTGGGTAA TGCGCCTTTG TTGGCATTAG TTCACAAGAT GAACAACTGC 
CTCAGCCAGA TGGAACAGTT TCCAGTCAAA GTACATGACT TCCCCAGTGG GAACGGGACA 
GGAGGCAGCT TTTCTCTCAA CAGAGGATCA CAGGCTTTAA AATTTTTCAA CACACACCAG 
TTAAAATGCC AGTTACAAAG ACATCCAGAC TGTGCAAATG TGAAGCAGTG GAAGGGCGGG 
CCGGTCAAGA TTGATCCCCT GGCTTTGGTA CAAGCTATTG AGAGATACCT TGTAGTTAGA 
GGGTATGGAA GAGTAAGAGA AGATGATGAA GACAGTGATG ATGATGGCTC TGATGAGGAA 
ATAGACGAGT CTCTGGCCGC TCAGTTCTTA AATTCCGGAA ACGTAAGGCA CAGGCTACAG 
TTTTATATTG GAGAACATTT GCTACCATAT AACATGACTG TATATCAGGC AGTCCGGCAG 
TTCAGTATAC AAGCTGAGGA TGAGCGGGAG TCCACAGATG ATGAGAGCAA CCCTCTGGGA 
AGAGCTGGTA TTTGGACAAA GACTCATACA ATATGGTACA AACCTGTGAG AGAGGATGAA 
GAAAGTAATA AAGATTGTGT TGGTGGTAAA AGAGGAAGAG CCCAAACGGC TCCAACAAAA 
ACATCCCCTA GAAATGCAAA AAAGCATGAT GAGTTATGGC ATGATGGAGT ATGCCCATCA 
GTATCCAATC CTTTAGAAGT TTACCTCATC TCCTCGCCAC CTGAGAATAT AACTTTTGAA 
GACCCGTCCT TAGATGTGAT TCTTCTTCTA AGAGTTTTAC ATGCCATCAG CCGATACTGG 
TATTACTTGT ATGATAATGC AATGTGCAAG GAGATTATTC CAACTAGTGA ATTTATTAAC 
AGTAAGTTGA CAGCAAAAGC AAATAGGCAG CTTCAAGATC CTTTAGTAAT CATGACAGGA 
AATATCCCAA CGTGGCTTAC TGAACTAGGA AAAACCTGCC CATTTTTCTT CCCTTTTGAT 
ACCCGGCAAA TGCTTTTTTA CGTAACTGCA TTTGATCGGG ACCGAGCAAT GCAAAGATTA 
CTTGATACCA ACCCAGAAAT CAACCAGTCG GATTCTCAAG ATAGCAGAGT TGCACCTAGA 
TTGGATAGAA AAAAACGTAC TGTGAACAGA GAGGAGCTGC TGAAACAAGC GGAGTCTGTA 
ATGCAGGATC TCGGTAGCTC ACGGGCCATG TTAGAAATCC AGTATGAAAA TGAGGTTGGT 
ACAGGTCTTG GGCCTACACT GGAGTTTTAT GCACTTGTAT CTCAGGAACT TCAGAGAGCT 
GACTTGGGTC TCTGGAGAGG TGAAGAAGTG ACTCTCAGCA ATCCAAAAGG AAGCCAAGAA 
GGGACCAAGT ATATTCAAAA CCTCCAGGGC CTGTTTGCAC TTCCCTTTGG TAGGACAGCT 
AAGCCAGCCC ATATCGCAAA GGTTAAAATG AAGTTTCGCT TCTTAGGAAA ATTAATGGCC 
AAGGCTATCA TGGATTTCAG ATTGGTGGAC CTTCCCCTTG GTTTACCCTT TTATAAGTGG 
ATGCTAAGGC AAGAAACTTC ACTGACGTCG CATGATTTGT TTGACATTGA CCCAGTTGTA 
GCCAGATCAG TGTATCACCT AGAAGACATT GTCAGACAGA AGAAAAGACT TGAGCAAGAT 
AAATCCCAGA CCAAAGAGAG TCTACAATAT GCATTAGAAA CCCTGACTAT GAATGGCTGC 
TCAGTTGAAG ATCTAGGATT GGATTTCACG CTGCCAGGGT TTCCCAACAT TGAACTGAAG 
AAAGGAGGAA AGGATATACC AGTTACCATC CACAATTTAG AGGAGTATCT AAGACTGGTT 
ATATTCTGGG CACTAAATGA AGGCGTTTCC AGGCAATTTG ATTCCTTCAG AGATGGATTT 
GAATCTGTCT TCCCACTTAG TCATCTTCAG TACTTCTACC CGGAAGAACT GGATCAGCTC 
TTGTGCGGCA GTAAAGCAGA CACGTGGGAT GCGAAGACAC TGATGGAGTG CTGCAGGCCG 
GACCACGGTT ATACTCATGA CAGTCGGGCT GTGAAGTTCT TGTTTGAGAT TCTCAGTAGT 
TTTGATAATG AGCAGCAGAG GTTGTTTCTC CAGTTTGTGA CAGGTAGCCC AAGATTGCCT 
GTTGGAGGCT TCCGGAGTCT GAATCCACCT CTGACAATTG TCCGGAAGAC CTTCGAGTCC 
ACGGAGAACC CCGACGACTT CTTGCCCTCT GTAATGACTT GTGTGAACTA CCTTAAGTTG 
CCGGACTACT CGAGCATTGA CATCATGCGT GAGAAACTGT TGATTGCAGC GAGAGAAGGG 
CAGCAGTCAT TCCATCTTTC CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005635859.1
CDS	188..6391
Translation	MSNRPNNNPGGSLRRSQRNTAGAQPQDDSIGGRSHLGQAKHKGYSPPESRKSNSKAPKVQSNTTSELSRGHLSKRSCSSSSAVIVPQPEDPDRANTSERQKTGQVPKKDNSRGVKRSASPDYNRTNSPSSAKKPKALQHTESPSETNKPHSKSKKRHLDQEQQLKSAQSPSTSKAHTRKSGATGGSRSQKRKRTESSCLKSGSVSESTGAEERSAKPTKLASKSAASAKAGCSTITDSSSAASTSSSSSAVASASSTVPPGARVKQGKDQSKARRSRSASSPSPRRSSREKEQSKTGGSSKFDWAARFSPKVSLPKTKLSLPGSSKSETSKPGPSGLQAKLASLRKSTKKRSESPPAELPSLRRSTRQKTTGSCASASRRGSGLGKRGAAEARRQEKMADPESSQETVNSSAARTDETPQGAAASSSVAGAVGMTTSGESESDDSEMGRLQALLEARGLPPHLFGPLGPRMSQLFHRTIGSGASSKAQQLLQGLQASDESQQLQAVIEMCQLLVMGNEETLGGFPVKSVVPALITLLQMEHNFDIMNHACRALTYMMEALPRSSAVVVDAIPVFLEKLQVIQCIDVAEQALTALEMLSRRHSKAILQAGGLADCLLYLEFFSINAQRNALAIAANCCQSITPDEFHFVADSLPLLTQRLTHQDKKSVESTCLCFARLVDNFQHEENLLQQVASKDLLTNVQQLLVVTPPILSSGMFIMVVRMFSLMCSNCPTLAVQLMKQNIAETLHFLLCGASNGSCQEQIDLVPRSPQELYELTSLICELMPCLPKEGIFAVDTMLKKGNAQNTDGAIWQWRDDRGLWHPYNRIDSRIIEAAHQVGEDEISLSTLGRVYTIDFNSMQQINEDTGTARAIQRKPNPLANTNTSGYSELKKDDARAQLMKEDPELAKSFIKTLFGVLYEVYSSSAGPAVRHKCLRAILRIIYFADAELLKDVLKNHAVSSHIASMLSSQDLKIVVGALQMAEILMQKLPDIFSVYFRREGVMHQVKHLAESESLLTSPPKACTNGSGSLGSTTSVSSGTATAATNAAADLGSPSLQHSRDDSLDLSPQGRLSDVLKRKRLPKRGPRRPKYSPPRDDDKVDNQAKSPTTTQSPKSSFLASLNPKTWGRLSAQSNSNNIEPARTAGVSGLARAASKDTISNNREKIKGWIKEQAHKFVERYFSSENMDGSNPALNVLQRLCAATEQLNLQVDGGAECLVEIRSIVSESDVSSFEIQHSGFVKQLLLYLTSKSEKDAVSREIRLKRFLHVFFSSPLPGEEPIGRVEPVGNAPLLALVHKMNNCLSQMEQFPVKVHDFPSGNGTGGSFSLNRGSQALKFFNTHQLKCQLQRHPDCANVKQWKGGPVKIDPLALVQAIERYLVVRGYGRVREDDEDSDDDGSDEEIDESLAAQFLNSGNVRHRLQFYIGEHLLPYNMTVYQAVRQFSIQAEDERESTDDESNPLGRAGIWTKTHTIWYKPVREDEESNKDCVGGKRGRAQTAPTKTSPRNAKKHDELWHDGVCPSVSNPLEVYLISSPPENITFEDPSLDVILLLRVLHAISRYWYYLYDNAMCKEIIPTSEFINSKLTAKANRQLQDPLVIMTGNIPTWLTELGKTCPFFFPFDTRQMLFYVTAFDRDRAMQRLLDTNPEINQSDSQDSRVAPRLDRKKRTVNREELLKQAESVMQDLGSSRAMLEIQYENEVGTGLGPTLEFYALVSQELQRADLGLWRGEEVTLSNPKGSQEGTKYIQNLQGLFALPFGRTAKPAHIAKVKMKFRFLGKLMAKAIMDFRLVDLPLGLPFYKWMLRQETSLTSHDLFDIDPVVARSVYHLEDIVRQKKRLEQDKSQTKESLQYALETLTMNGCSVEDLGLDFTLPGFPNIELKKGGKDIPVTIHNLEEYLRLVIFWALNEGVSRQFDSFRDGFESVFPLSHLQYFYPEELDQLLCGSKADTWDAKTLMECCRPDHGYTHDSRAVKFLFEILSSFDNEQQRLFLQFVTGSPRLPVGGFRSLNPPLTIVRKTFESTENPDDFLPSVMTCVNYLKLPDYSSIDIMREKLLIAAREGQQSFHLS

Target ORF information:

RefSeq Version	XM_005635802.2
Organism	Canis lupus familiaris(Dog)
Definition	Canis lupus familiaris thyroid hormone receptor interactor 12 (TRIP12), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005635802.2

ORF Insert Sequence:

ATGTCCAACC GGCCTAATAA CAATCCAGGG GGGTCACTGC GACGTTCACA GAGGAACACT 
GCCGGGGCCC AACCACAAGA CGACTCGATA GGAGGAAGGT CACATTTAGG GCAGGCAAAA 
CATAAGGGAT ATAGCCCTCC TGAGAGTAGA AAATCTAATT CTAAGGCACC CAAAGTGCAG 
TCTAATACTA CTTCTGAACT GTCAAGGGGA CACCTTTCTA AAAGAAGCTG CAGTTCATCA 
TCTGCTGTCA TAGTTCCACA ACCAGAGGAT CCAGACAGAG CCAATACTTC AGAAAGGCAA 
AAAACGGGGC AGGTGCCTAA GAAAGACAAT TCTCGAGGAG TGAAACGCAG TGCTAGTCCA 
GACTACAACA GGACCAATTC TCCTAGCTCT GCCAAAAAAC CAAAAGCACT TCAGCATACT 
GAATCTCCCT CAGAAACAAA TAAGCCACAT AGTAAGTCGA AGAAGAGACA TTTAGACCAG 
GAGCAACAAC TGAAATCTGC ACAGTCGCCA TCAACAAGTA AGGCTCACAC CAGAAAGAGT 
GGGGCCACCG GCGGCTCACG GAGTCAGAAA AGAAAGAGGA CAGAGAGTTC TTGTCTAAAG 
AGTGGTTCTG TGTCTGAGTC AACTGGTGCC GAAGAGAGAT CTGCAAAACC CACCAAGCTG 
GCTTCAAAAT CCGCCGCCTC GGCCAAAGCT GGGTGCAGCA CCATCACTGA TTCTTCCTCC 
GCTGCCTCTA CTTCCTCCTC ATCTTCTGCT GTCGCCTCGG CCTCCTCCAC TGTACCACCA 
GGGGCCAGGG TGAAACAAGG AAAAGACCAG AGCAAGGCCA GGCGTTCCCG TTCAGCATCC 
AGTCCCAGTC CCAGAAGAAG TAGCAGGGAA AAGGAACAGA GTAAAACTGG TGGCTCTTCA 
AAATTCGATT GGGCTGCTCG TTTCAGCCCT AAAGTTAGCC TTCCTAAAAC CAAACTGTCT 
CTTCCAGGGT CTTCTAAGTC AGAGACATCA AAACCTGGAC CTTCTGGATT ACAGGCCAAA 
TTAGCAAGTT TAAGAAAATC TACCAAGAAG CGCAGTGAAT CCCCACCTGC TGAGCTCCCC 
AGTTTGAGGC GGAGCACACG CCAAAAGACC ACGGGCTCCT GTGCTAGTGC CAGTCGGCGA 
GGCTCTGGCC TGGGCAAAAG AGGAGCAGCT GAAGCTCGTC GACAGGAGAA GATGGCGGAC 
CCTGAAAGCA GCCAGGAGAC CGTGAATTCT TCAGCTGCAC GGACAGACGA AACTCCCCAA 
GGAGCTGCAG CCTCTAGTTC TGTTGCAGGG GCTGTGGGCA TGACCACCTC TGGGGAGAGT 
GAATCCGATG ATTCTGAGAT GGGACGACTA CAAGCTTTGT TAGAGGCCAG GGGTCTTCCT 
CCTCATCTGT TTGGCCCTCT CGGTCCTCGG ATGTCACAAC TTTTCCATAG AACAATTGGA 
AGCGGAGCTA GCTCTAAGGC CCAGCAGCTT CTGCAAGGAC TACAAGCAAG TGATGAAAGC 
CAGCAACTCC AGGCAGTTAT TGAAATGTGT CAGTTATTGG TTATGGGAAA TGAGGAGACG 
CTGGGAGGGT TTCCGGTCAA GAGTGTCGTT CCAGCTTTGA TAACATTACT GCAGATGGAG 
CATAATTTCG ATATTATGAA CCATGCTTGT CGAGCCTTAA CATATATGAT GGAAGCACTT 
CCTCGGTCAT CTGCTGTTGT AGTAGATGCA ATTCCTGTCT TTTTAGAAAA GCTGCAAGTT 
ATTCAGTGTA TTGATGTCGC AGAGCAGGCC CTGACTGCCT TGGAGATGTT ATCACGCAGA 
CACAGTAAAG CCATTCTACA GGCGGGTGGT TTGGCAGACT GCTTGCTGTA TCTAGAATTC 
TTCAGCATAA ATGCCCAAAG AAATGCACTA GCAATTGCAG CCAATTGCTG CCAGAGTATC 
ACGCCAGATG AATTTCATTT TGTGGCAGAT TCCCTCCCGT TGCTTACCCA AAGGCTAACC 
CATCAGGACA AAAAGTCAGT TGAAAGCACT TGCCTTTGTT TTGCACGTCT AGTGGACAAC 
TTCCAACATG AGGAGAATTT ACTCCAGCAG GTTGCCTCCA AAGATCTGCT TACGAATGTT 
CAACAACTCT TGGTAGTGAC TCCACCCATT TTAAGTTCTG GGATGTTTAT AATGGTGGTT 
CGCATGTTTT CTCTGATGTG TTCCAACTGT CCAACTTTAG CTGTTCAACT TATGAAGCAA 
AACATTGCAG AAACACTTCA CTTTCTCCTG TGTGGTGCCT CTAATGGAAG TTGTCAGGAA 
CAGATTGATC TTGTTCCACG AAGTCCACAA GAACTGTATG AACTGACATC TCTGATTTGC 
GAACTTATGC CATGTTTACC AAAAGAAGGC ATTTTTGCAG TTGATACCAT GCTGAAGAAG 
GGAAATGCGC AGAACACAGA CGGTGCGATT TGGCAGTGGC GTGATGACCG TGGCCTTTGG 
CATCCCTATA ACAGGATTGA CAGCCGGATC ATTGAGGCAG CCCATCAGGT CGGTGAGGAT 
GAGATAAGCT TGTCCACTCT GGGACGAGTT TATACTATTG ATTTTAATTC TATGCAGCAA 
ATCAATGAGG ACACGGGAAC AGCACGTGCC ATTCAGAGAA AACCTAACCC CTTAGCCAAT 
ACTAACACTA GTGGATATTC AGAGTTAAAG AAGGATGATG CTCGAGCACA GCTTATGAAA 
GAGGATCCGG AACTGGCTAA GTCTTTTATT AAGACATTAT TTGGTGTTCT TTATGAAGTG 
TATAGTTCCT CAGCAGGACC TGCGGTCAGA CATAAGTGCC TTAGAGCAAT TCTTAGGATA 
ATTTATTTTG CGGATGCTGA ACTTCTGAAG GATGTCCTGA AAAATCATGC TGTTTCAAGT 
CACATTGCTT CCATGCTGTC AAGCCAAGAC CTGAAGATAG TAGTTGGAGC ACTTCAGATG 
GCAGAAATCT TAATGCAGAA GTTACCTGAT ATTTTTAGTG TTTACTTCAG AAGAGAAGGT 
GTAATGCATC AAGTAAAACA CTTAGCAGAA TCAGAGTCTT TGTTGACAAG TCCACCAAAG 
GCATGTACGA ATGGGTCAGG ATCCCTGGGA TCCACGACGT CAGTCAGCAG TGGAACAGCC 
ACGGCCGCCA CCAATGCTGC AGCTGACCTG GGGTCCCCCA GCTTGCAGCA CAGCAGAGAT 
GACTCTTTAG ATCTGAGCCC TCAAGGTCGA TTAAGTGATG TTCTAAAGAG AAAACGGCTG 
CCAAAACGAG GGCCAAGAAG GCCAAAGTAT TCACCTCCAA GAGATGATGA CAAAGTAGAC 
AATCAAGCTA AAAGCCCTAC CACTACTCAG TCACCTAAAT CTTCTTTCCT GGCAAGCTTG 
AATCCAAAAA CGTGGGGAAG GTTAAGCGCA CAGTCCAACA GCAACAACAT CGAACCAGCA 
CGAACTGCGG GAGTCAGTGG TCTTGCCAGG GCCGCCTCCA AGGACACCAT ATCCAATAAC 
AGAGAAAAAA TTAAAGGTTG GATTAAGGAA CAGGCGCATA AATTTGTAGA GCGTTATTTC 
AGTTCTGAGA ACATGGATGG AAGCAACCCT GCACTGAATG TCCTTCAGAG ACTTTGTGCT 
GCAACTGAAC AACTCAACCT CCAGGTGGAT GGTGGAGCTG AGTGCCTTGT AGAAATCCGT 
AGCATAGTCT CAGAGTCTGA TGTTTCATCA TTTGAAATCC AACATAGTGG ATTTGTGAAG 
CAGCTGTTAC TTTATTTGAC ATCTAAAAGT GAAAAGGATG CTGTGAGCCG AGAGATCAGA 
TTAAAGAGAT TCCTTCACGT ATTTTTTTCT TCACCACTTC CTGGAGAAGA GCCCATTGGA 
AGAGTAGAAC CAGTGGGTAA TGCGCCTTTG TTGGCATTAG TTCACAAGAT GAACAACTGC 
CTCAGCCAGA TGGAACAGTT TCCAGTCAAA GTACATGACT TCCCCAGTGG GAACGGGACA 
GGAGGCAGCT TTTCTCTCAA CAGAGGATCA CAGGCTTTAA AATTTTTCAA CACACACCAG 
TTAAAATGCC AGTTACAAAG ACATCCAGAC TGTGCAAATG TGAAGCAGTG GAAGGGCGGG 
CCGGTCAAGA TTGATCCCCT GGCTTTGGTA CAAGCTATTG AGAGATACCT TGTAGTTAGA 
GGGTATGGAA GAGTAAGAGA AGATGATGAA GACAGTGATG ATGATGGCTC TGATGAGGAA 
ATAGACGAGT CTCTGGCCGC TCAGTTCTTA AATTCCGGAA ACGTAAGGCA CAGGCTACAG 
TTTTATATTG GAGAACATTT GCTACCATAT AACATGACTG TATATCAGGC AGTCCGGCAG 
TTCAGTATAC AAGCTGAGGA TGAGCGGGAG TCCACAGATG ATGAGAGCAA CCCTCTGGGA 
AGAGCTGGTA TTTGGACAAA GACTCATACA ATATGGTACA AACCTGTGAG AGAGGATGAA 
GAAAGTAATA AAGATTGTGT TGGTGGTAAA AGAGGAAGAG CCCAAACGGC TCCAACAAAA 
ACATCCCCTA GAAATGCAAA AAAGCATGAT GAGTTATGGC ATGATGGAGT ATGCCCATCA 
GTATCCAATC CTTTAGAAGT TTACCTCATC TCCTCGCCAC CTGAGAATAT AACTTTTGAA 
GACCCGTCCT TAGATGTGAT TCTTCTTCTA AGAGTTTTAC ATGCCATCAG CCGATACTGG 
TATTACTTGT ATGATAATGC AATGTGCAAG GAGATTATTC CAACTAGTGA ATTTATTAAC 
AGTAAGTTGA CAGCAAAAGC AAATAGGCAG CTTCAAGATC CTTTAGTAAT CATGACAGGA 
AATATCCCAA CGTGGCTTAC TGAACTAGGA AAAACCTGCC CATTTTTCTT CCCTTTTGAT 
ACCCGGCAAA TGCTTTTTTA CGTAACTGCA TTTGATCGGG ACCGAGCAAT GCAAAGATTA 
CTTGATACCA ACCCAGAAAT CAACCAGTCG GATTCTCAAG ATAGCAGAGT TGCACCTAGA 
TTGGATAGAA AAAAACGTAC TGTGAACAGA GAGGAGCTGC TGAAACAAGC GGAGTCTGTA 
ATGCAGGATC TCGGTAGCTC ACGGGCCATG TTAGAAATCC AGTATGAAAA TGAGGTTGGT 
ACAGGTCTTG GGCCTACACT GGAGTTTTAT GCACTTGTAT CTCAGGAACT TCAGAGAGCT 
GACTTGGGTC TCTGGAGAGG TGAAGAAGTG ACTCTCAGCA ATCCAAAAGG AAGCCAAGAA 
GGGACCAAGT ATATTCAAAA CCTCCAGGGC CTGTTTGCAC TTCCCTTTGG TAGGACAGCT 
AAGCCAGCCC ATATCGCAAA GGTTAAAATG AAGTTTCGCT TCTTAGGAAA ATTAATGGCC 
AAGGCTATCA TGGATTTCAG ATTGGTGGAC CTTCCCCTTG GTTTACCCTT TTATAAGTGG 
ATGCTAAGGC AAGAAACTTC ACTGACGTCG CATGATTTGT TTGACATTGA CCCAGTTGTA 
GCCAGATCAG TGTATCACCT AGAAGACATT GTCAGACAGA AGAAAAGACT TGAGCAAGAT 
AAATCCCAGA CCAAAGAGAG TCTACAATAT GCATTAGAAA CCCTGACTAT GAATGGCTGC 
TCAGTTGAAG ATCTAGGATT GGATTTCACG CTGCCAGGGT TTCCCAACAT TGAACTGAAG 
AAAGGAGGAA AGGATATACC AGTTACCATC CACAATTTAG AGGAGTATCT AAGACTGGTT 
ATATTCTGGG CACTAAATGA AGGCGTTTCC AGGCAATTTG ATTCCTTCAG AGATGGATTT 
GAATCTGTCT TCCCACTTAG TCATCTTCAG TACTTCTACC CGGAAGAACT GGATCAGCTC 
TTGTGCGGCA GTAAAGCAGA CACGTGGGAT GCGAAGACAC TGATGGAGTG CTGCAGGCCG 
GACCACGGTT ATACTCATGA CAGTCGGGCT GTGAAGTTCT TGTTTGAGAT TCTCAGTAGT 
TTTGATAATG AGCAGCAGAG GTTGTTTCTC CAGTTTGTGA CAGGTAGCCC AAGATTGCCT 
GTTGGAGGCT TCCGGAGTCT GAATCCACCT CTGACAATTG TCCGGAAGAC CTTCGAGTCC 
ACGGAGAACC CCGACGACTT CTTGCCCTCT GTAATGACTT GTGTGAACTA CCTTAAGTTG 
CCGGACTACT CGAGCATTGA CATCATGCGT GAGAAACTGT TGATTGCAGC GAGAGAAGGG 
CAGCAGTCAT TCCATCTTTC CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TRIP12 ( XM_005635803.2 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_005635860.1 Canis lupus familiaris thyroid hormone receptor interactor 12 (TRIP12), transcript variant X4, mRNA.

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CloneID	OCe25763
Clone ID Related Accession (Same CDS sequence)	XM_005635803.2 , XM_022409665.1
Accession Version	XM_005635803.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6192bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-09-16
Organism	Canis lupus familiaris(Dog)
Product	E3 ubiquitin-protein ligase TRIP12 isoform X3
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726103.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 17, 2015 this sequence version replaced XM_005635803.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCCAACC GGCCTAATAA CAATCCAGGG GGGTCACTGC GACGTTCACA GAGGAACACT 
GCCGGGGCCC AACCACAAGA CGACTCGATA GGAGGAAGGT CACATTTAGG GCAGGCAAAA 
CATAAGGGAT ATAGCCCTCC TGAGAGTAGA AAATCTAATT CTAAGGCACC CAAAGTGCAG 
TCTAATACTA CTTCTGAACT GTCAAGGGGA CACCTTTCTA AAAGAAGCTG CAGTTCATCA 
TCTGCTGTCA TAGTTCCACA ACCAGAGGAT CCAGACAGAG CCAATACTTC AGAAAGGCAA 
AAAACGGGGC AGGTGCCTAA GAAAGACAAT TCTCGAGGAG TGAAACGCAG TGCTAGTCCA 
GACTACAACA GGACCAATTC TCCTAGCTCT GCCAAAAAAC CAAAAGCACT TCAGCATACT 
GAATCTCCCT CAGAAACAAA TAAGCCACAT AGTAAGTCGA AGAAGAGACA TTTAGACCAG 
GAGCAACAAC TGAAATCTGC ACAGTCGCCA TCAACAAGTA AGGCTCACAC CAGAAAGAGT 
GGGGCCACCG GCGGCTCACG GAGTCAGAAA AGAAAGAGGA CAGAGAGTTC TTGTCTAAAG 
AGTGGTTCTG TGTCTGAGTC AACTGGTGCC GAAGAGAGAT CTGCAAAACC CACCAAGCTG 
GCTTCAAAAT CCGCCGCCTC GGCCAAAGCT GGGTGCAGCA CCATCACTGA TTCTTCCTCC 
GCTGCCTCTA CTTCCTCCTC ATCTTCTGCT GTCGCCTCGG CCTCCTCCAC TGTACCACCA 
GGGGCCAGGG TGAAACAAGG AAAAGACCAG AGCAAGGCCA GGCGTTCCCG TTCAGCATCC 
AGTCCCAGTC CCAGAAGAAG TAGCAGGGAA AAGGAACAGA GTAAAACTGG TGGCTCTTCA 
AAATTCGATT GGGCTGCTCG TTTCAGCCCT AAAGTTAGCC TTCCTAAAAC CAAACTGTCT 
CTTCCAGGGT CTTCTAAGTC AGAGACATCA AAACCTGGAC CTTCTGGATT ACAGGCCAAA 
TTAGCAAGTT TAAGAAAATC TACCAAGAAG CGCAGTGAAT CCCCACCTGC TGAGCTCCCC 
AGTTTGAGGC GGAGCACACG CCAAAAGACC ACGGGCTCCT GTGCTAGTGC CAGTCGGCGA 
GGCTCTGGCC TGGGCAAAAG AGGAGCAGCT GAAGCTCGTC GACAGGAGAA GATGGCGGAC 
CCTGAAAGCA GCCAGGAGAC CGTGAATTCT TCAGCTGCAC GGACAGACGA AACTCCCCAA 
GGAGCTGCAG CCTCTAGTTC TGTTGCAGGG GCTGTGGGCA TGACCACCTC TGGGGAGAGT 
GAATCCGATG ATTCTGAGAT GGGACGACTA CAAGCTTTGT TAGAGGCCAG GGGTCTTCCT 
CCTCATCTGT TTGGCCCTCT CGGTCCTCGG ATGTCACAAC TTTTCCATAG AACAATTGGA 
AGCGGAGCTA GCTCTAAGGC CCAGCAGCTT CTGCAAGGAC TACAAGCAAG TGATGAAAGC 
CAGCAACTCC AGGCAGTTAT TGAAATGTGT CAGTTATTGG TTATGGGAAA TGAGGAGACG 
CTGGGAGGGT TTCCGGTCAA GAGTGTCGTT CCAGCTTTGA TAACATTACT GCAGATGGAG 
CATAATTTCG ATATTATGAA CCATGCTTGT CGAGCCTTAA CATATATGAT GGAAGCACTT 
CCTCGGTCAT CTGCTGTTGT AGTAGATGCA ATTCCTGTCT TTTTAGAAAA GCTGCAAGTT 
ATTCAGTGTA TTGATGTCGC AGAGCAGGCC CTGACTGCCT TGGAGATGTT ATCACGCAGA 
CACAGTAAAG CCATTCTACA GGCGGGTGGT TTGGCAGACT GCTTGCTGTA TCTAGAATTC 
TTCAGCATAA ATGCCCAAAG AAATGCACTA GCAATTGCAG CCAATTGCTG CCAGAGTATC 
ACGCCAGATG AATTTCATTT TGTGGCAGAT TCCCTCCCGT TGCTTACCCA AAGGCTAACC 
CATCAGGACA AAAAGTCAGT TGAAAGCACT TGCCTTTGTT TTGCACGTCT AGTGGACAAC 
TTCCAACATG AGGAGAATTT ACTCCAGCAG GTTGCCTCCA AAGATCTGCT TACGAATGTT 
CAACAACTCT TGGTAGTGAC TCCACCCATT TTAAGTTCTG GGATGTTTAT AATGGTGGTT 
CGCATGTTTT CTCTGATGTG TTCCAACTGT CCAACTTTAG CTGTTCAACT TATGAAGCAA 
AACATTGCAG AAACACTTCA CTTTCTCCTG TGTGGTGCCT CTAATGGAAG TTGTCAGGAA 
CAGATTGATC TTGTTCCACG AAGTCCACAA GAACTGTATG AACTGACATC TCTGATTTGC 
GAACTTATGC CATGTTTACC AAAAGAAGGC ATTTTTGCAG TTGATACCAT GCTGAAGAAG 
GGAAATGCGC AGAACACAGA CGGTGCGATT TGGCAGTGGC GTGATGACCG TGGCCTTTGG 
CATCCCTATA ACAGGATTGA CAGCCGGATC ATTGAGGTAG CAGCCCATCA GGTCGGTGAG 
GATGAGATAA GCTTGTCCAC TCTGGGACGA GTTTATACTA TTGATTTTAA TTCTATGCAG 
CAAATCAATG AGGACACGGG AACAGCACGT GCCATTCAGA GAAAACCTAA CCCCTTAGCC 
AATACTAACA CTAGTGGATA TTCAGAGTTA AAGAAGGATG ATGCTCGAGC ACAGCTTATG 
AAAGAGGATC CGGAACTGGC TAAGTCTTTT ATTAAGACAT TATTTGGTGT TCTTTATGAA 
GTGTATAGTT CCTCAGCAGG ACCTGCGGTC AGACATAAGT GCCTTAGAGC AATTCTTAGG 
ATAATTTATT TTGCGGATGC TGAACTTCTG AAGGATGTCC TGAAAAATCA TGCTGTTTCA 
AGTCACATTG CTTCCATGCT GTCAAGCCAA GACCTGAAGA TAGTAGTTGG AGCACTTCAG 
ATGGCAGAAA TCTTAATGCA GAAGTTACCT GATATTTTTA GTGTTTACTT CAGAAGAGAA 
GGTGTAATGC ATCAAGTAAA ACACTTAGCA GAATCAGAGT CTTTGTTGAC AAGTCCACCA 
AAGGCATGTA CGAATGGGTC AGGATCCCTG GGATCCACGA CGTCAGTCAG CAGTGGAACA 
GCCACGGCCG CCACCAATGC TGCAGCTGAC CTGGGGTCCC CCAGCTTGCA GCACAGCAGA 
GATGACTCTT TAGATCTGAG CCCTCAAGGT CGATTAAGTG ATGTTCTAAA GAGAAAACGG 
CTGCCAAAAC GAGGGCCAAG AAGGCCAAAG TATTCACCTC CAAGAGATGA TGACAAAGTA 
GACAATCAAG CTAAAAGCCC TACCACTACT CAGTCACCTA AATCTTCTTT CCTGGCAAGC 
TTGAATCCAA AAACGTGGGG AAGGTTAAGC GCACAGTCCA ACAGCAACAA CATCGAACCA 
GCACGAACTG CGGGAGTCAG TGGTCTTGCC AGGGCCGCCT CCAAGGACAC CATATCCAAT 
AACAGAGAAA AAATTAAAGG TTGGATTAAG GAACAGGCGC ATAAATTTGT AGAGCGTTAT 
TTCAGTTCTG AGAACATGGA TGGAAGCAAC CCTGCACTGA ATGTCCTTCA GAGACTTTGT 
GCTGCAACTG AACAACTCAA CCTCCAGGTG GATGGTGGAG CTGAGTGCCT TGTAGAAATC 
CGTAGCATAG TCTCAGAGTC TGATGTTTCA TCATTTGAAA TCCAACATAG TGGATTTGTG 
AAGCAGCTGT TACTTTATTT GACATCTAAA AGTGAAAAGG ATGCTGTGAG CCGAGAGATC 
AGATTAAAGA GATTCCTTCA CGTATTTTTT TCTTCACCAC TTCCTGGAGA AGAGCCCATT 
GGAAGAGTAG AACCAGTGGG TAATGCGCCT TTGTTGGCAT TAGTTCACAA GATGAACAAC 
TGCCTCAGCC AGATGGAACA GTTTCCAGTC AAAGTACATG ACTTCCCCAG TGGGAACGGG 
ACAGGAGGCA GAGGATCACA GGCTTTAAAA TTTTTCAACA CACACCAGTT AAAATGCCAG 
TTACAAAGAC ATCCAGACTG TGCAAATGTG AAGCAGTGGA AGGGCGGGCC GGTCAAGATT 
GATCCCCTGG CTTTGGTACA AGCTATTGAG AGATACCTTG TAGTTAGAGG GTATGGAAGA 
GTAAGAGAAG ATGATGAAGA CAGTGATGAT GATGGCTCTG ATGAGGAAAT AGACGAGTCT 
CTGGCCGCTC AGTTCTTAAA TTCCGGAAAC GTAAGGCACA GGCTACAGTT TTATATTGGA 
GAACATTTGC TACCATATAA CATGACTGTA TATCAGGCAG TCCGGCAGTT CAGTATACAA 
GCTGAGGATG AGCGGGAGTC CACAGATGAT GAGAGCAACC CTCTGGGAAG AGCTGGTATT 
TGGACAAAGA CTCATACAAT ATGGTACAAA CCTGTGAGAG AGGATGAAGA AAGTAATAAA 
GATTGTGTTG GTGGTAAAAG AGGAAGAGCC CAAACGGCTC CAACAAAAAC ATCCCCTAGA 
AATGCAAAAA AGCATGATGA GTTATGGCAT GATGGAGTAT GCCCATCAGT ATCCAATCCT 
TTAGAAGTTT ACCTCATCTC CTCGCCACCT GAGAATATAA CTTTTGAAGA CCCGTCCTTA 
GATGTGATTC TTCTTCTAAG AGTTTTACAT GCCATCAGCC GATACTGGTA TTACTTGTAT 
GATAATGCAA TGTGCAAGGA GATTATTCCA ACTAGTGAAT TTATTAACAG TAAGTTGACA 
GCAAAAGCAA ATAGGCAGCT TCAAGATCCT TTAGTAATCA TGACAGGAAA TATCCCAACG 
TGGCTTACTG AACTAGGAAA AACCTGCCCA TTTTTCTTCC CTTTTGATAC CCGGCAAATG 
CTTTTTTACG TAACTGCATT TGATCGGGAC CGAGCAATGC AAAGATTACT TGATACCAAC 
CCAGAAATCA ACCAGTCGGA TTCTCAAGAT AGCAGAGTTG CACCTAGATT GGATAGAAAA 
AAACGTACTG TGAACAGAGA GGAGCTGCTG AAACAAGCGG AGTCTGTAAT GCAGGATCTC 
GGTAGCTCAC GGGCCATGTT AGAAATCCAG TATGAAAATG AGGTTGGTAC AGGTCTTGGG 
CCTACACTGG AGTTTTATGC ACTTGTATCT CAGGAACTTC AGAGAGCTGA CTTGGGTCTC 
TGGAGAGGTG AAGAAGTGAC TCTCAGCAAT CCAAAAGGAA GCCAAGAAGG GACCAAGTAT 
ATTCAAAACC TCCAGGGCCT GTTTGCACTT CCCTTTGGTA GGACAGCTAA GCCAGCCCAT 
ATCGCAAAGG TTAAAATGAA GTTTCGCTTC TTAGGAAAAT TAATGGCCAA GGCTATCATG 
GATTTCAGAT TGGTGGACCT TCCCCTTGGT TTACCCTTTT ATAAGTGGAT GCTAAGGCAA 
GAAACTTCAC TGACGTCGCA TGATTTGTTT GACATTGACC CAGTTGTAGC CAGATCAGTG 
TATCACCTAG AAGACATTGT CAGACAGAAG AAAAGACTTG AGCAAGATAA ATCCCAGACC 
AAAGAGAGTC TACAATATGC ATTAGAAACC CTGACTATGA ATGGCTGCTC AGTTGAAGAT 
CTAGGATTGG ATTTCACGCT GCCAGGGTTT CCCAACATTG AACTGAAGAA AGGAGGAAAG 
GATATACCAG TTACCATCCA CAATTTAGAG GAGTATCTAA GACTGGTTAT ATTCTGGGCA 
CTAAATGAAG GCGTTTCCAG GCAATTTGAT TCCTTCAGAG ATGGATTTGA ATCTGTCTTC 
CCACTTAGTC ATCTTCAGTA CTTCTACCCG GAAGAACTGG ATCAGCTCTT GTGCGGCAGT 
AAAGCAGACA CGTGGGATGC GAAGACACTG ATGGAGTGCT GCAGGCCGGA CCACGGTTAT 
ACTCATGACA GTCGGGCTGT GAAGTTCTTG TTTGAGATTC TCAGTAGTTT TGATAATGAG 
CAGCAGAGGT TGTTTCTCCA GTTTGTGACA GGTAGCCCAA GATTGCCTGT TGGAGGCTTC 
CGGAGTCTGA ATCCACCTCT GACAATTGTC CGGAAGACCT TCGAGTCCAC GGAGAACCCC 
GACGACTTCT TGCCCTCTGT AATGACTTGT GTGAACTACC TTAAGTTGCC GGACTACTCG 
AGCATTGACA TCATGCGTGA GAAACTGTTG ATTGCAGCGA GAGAAGGGCA GCAGTCATTC 
CATCTTTCCT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005635860.1
CDS	188..6379
Translation	MSNRPNNNPGGSLRRSQRNTAGAQPQDDSIGGRSHLGQAKHKGYSPPESRKSNSKAPKVQSNTTSELSRGHLSKRSCSSSSAVIVPQPEDPDRANTSERQKTGQVPKKDNSRGVKRSASPDYNRTNSPSSAKKPKALQHTESPSETNKPHSKSKKRHLDQEQQLKSAQSPSTSKAHTRKSGATGGSRSQKRKRTESSCLKSGSVSESTGAEERSAKPTKLASKSAASAKAGCSTITDSSSAASTSSSSSAVASASSTVPPGARVKQGKDQSKARRSRSASSPSPRRSSREKEQSKTGGSSKFDWAARFSPKVSLPKTKLSLPGSSKSETSKPGPSGLQAKLASLRKSTKKRSESPPAELPSLRRSTRQKTTGSCASASRRGSGLGKRGAAEARRQEKMADPESSQETVNSSAARTDETPQGAAASSSVAGAVGMTTSGESESDDSEMGRLQALLEARGLPPHLFGPLGPRMSQLFHRTIGSGASSKAQQLLQGLQASDESQQLQAVIEMCQLLVMGNEETLGGFPVKSVVPALITLLQMEHNFDIMNHACRALTYMMEALPRSSAVVVDAIPVFLEKLQVIQCIDVAEQALTALEMLSRRHSKAILQAGGLADCLLYLEFFSINAQRNALAIAANCCQSITPDEFHFVADSLPLLTQRLTHQDKKSVESTCLCFARLVDNFQHEENLLQQVASKDLLTNVQQLLVVTPPILSSGMFIMVVRMFSLMCSNCPTLAVQLMKQNIAETLHFLLCGASNGSCQEQIDLVPRSPQELYELTSLICELMPCLPKEGIFAVDTMLKKGNAQNTDGAIWQWRDDRGLWHPYNRIDSRIIEVAAHQVGEDEISLSTLGRVYTIDFNSMQQINEDTGTARAIQRKPNPLANTNTSGYSELKKDDARAQLMKEDPELAKSFIKTLFGVLYEVYSSSAGPAVRHKCLRAILRIIYFADAELLKDVLKNHAVSSHIASMLSSQDLKIVVGALQMAEILMQKLPDIFSVYFRREGVMHQVKHLAESESLLTSPPKACTNGSGSLGSTTSVSSGTATAATNAAADLGSPSLQHSRDDSLDLSPQGRLSDVLKRKRLPKRGPRRPKYSPPRDDDKVDNQAKSPTTTQSPKSSFLASLNPKTWGRLSAQSNSNNIEPARTAGVSGLARAASKDTISNNREKIKGWIKEQAHKFVERYFSSENMDGSNPALNVLQRLCAATEQLNLQVDGGAECLVEIRSIVSESDVSSFEIQHSGFVKQLLLYLTSKSEKDAVSREIRLKRFLHVFFSSPLPGEEPIGRVEPVGNAPLLALVHKMNNCLSQMEQFPVKVHDFPSGNGTGGRGSQALKFFNTHQLKCQLQRHPDCANVKQWKGGPVKIDPLALVQAIERYLVVRGYGRVREDDEDSDDDGSDEEIDESLAAQFLNSGNVRHRLQFYIGEHLLPYNMTVYQAVRQFSIQAEDERESTDDESNPLGRAGIWTKTHTIWYKPVREDEESNKDCVGGKRGRAQTAPTKTSPRNAKKHDELWHDGVCPSVSNPLEVYLISSPPENITFEDPSLDVILLLRVLHAISRYWYYLYDNAMCKEIIPTSEFINSKLTAKANRQLQDPLVIMTGNIPTWLTELGKTCPFFFPFDTRQMLFYVTAFDRDRAMQRLLDTNPEINQSDSQDSRVAPRLDRKKRTVNREELLKQAESVMQDLGSSRAMLEIQYENEVGTGLGPTLEFYALVSQELQRADLGLWRGEEVTLSNPKGSQEGTKYIQNLQGLFALPFGRTAKPAHIAKVKMKFRFLGKLMAKAIMDFRLVDLPLGLPFYKWMLRQETSLTSHDLFDIDPVVARSVYHLEDIVRQKKRLEQDKSQTKESLQYALETLTMNGCSVEDLGLDFTLPGFPNIELKKGGKDIPVTIHNLEEYLRLVIFWALNEGVSRQFDSFRDGFESVFPLSHLQYFYPEELDQLLCGSKADTWDAKTLMECCRPDHGYTHDSRAVKFLFEILSSFDNEQQRLFLQFVTGSPRLPVGGFRSLNPPLTIVRKTFESTENPDDFLPSVMTCVNYLKLPDYSSIDIMREKLLIAAREGQQSFHLS

Target ORF information:

RefSeq Version	XM_005635803.2
Organism	Canis lupus familiaris(Dog)
Definition	Canis lupus familiaris thyroid hormone receptor interactor 12 (TRIP12), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005635803.2

ORF Insert Sequence:

ATGTCCAACC GGCCTAATAA CAATCCAGGG GGGTCACTGC GACGTTCACA GAGGAACACT 
GCCGGGGCCC AACCACAAGA CGACTCGATA GGAGGAAGGT CACATTTAGG GCAGGCAAAA 
CATAAGGGAT ATAGCCCTCC TGAGAGTAGA AAATCTAATT CTAAGGCACC CAAAGTGCAG 
TCTAATACTA CTTCTGAACT GTCAAGGGGA CACCTTTCTA AAAGAAGCTG CAGTTCATCA 
TCTGCTGTCA TAGTTCCACA ACCAGAGGAT CCAGACAGAG CCAATACTTC AGAAAGGCAA 
AAAACGGGGC AGGTGCCTAA GAAAGACAAT TCTCGAGGAG TGAAACGCAG TGCTAGTCCA 
GACTACAACA GGACCAATTC TCCTAGCTCT GCCAAAAAAC CAAAAGCACT TCAGCATACT 
GAATCTCCCT CAGAAACAAA TAAGCCACAT AGTAAGTCGA AGAAGAGACA TTTAGACCAG 
GAGCAACAAC TGAAATCTGC ACAGTCGCCA TCAACAAGTA AGGCTCACAC CAGAAAGAGT 
GGGGCCACCG GCGGCTCACG GAGTCAGAAA AGAAAGAGGA CAGAGAGTTC TTGTCTAAAG 
AGTGGTTCTG TGTCTGAGTC AACTGGTGCC GAAGAGAGAT CTGCAAAACC CACCAAGCTG 
GCTTCAAAAT CCGCCGCCTC GGCCAAAGCT GGGTGCAGCA CCATCACTGA TTCTTCCTCC 
GCTGCCTCTA CTTCCTCCTC ATCTTCTGCT GTCGCCTCGG CCTCCTCCAC TGTACCACCA 
GGGGCCAGGG TGAAACAAGG AAAAGACCAG AGCAAGGCCA GGCGTTCCCG TTCAGCATCC 
AGTCCCAGTC CCAGAAGAAG TAGCAGGGAA AAGGAACAGA GTAAAACTGG TGGCTCTTCA 
AAATTCGATT GGGCTGCTCG TTTCAGCCCT AAAGTTAGCC TTCCTAAAAC CAAACTGTCT 
CTTCCAGGGT CTTCTAAGTC AGAGACATCA AAACCTGGAC CTTCTGGATT ACAGGCCAAA 
TTAGCAAGTT TAAGAAAATC TACCAAGAAG CGCAGTGAAT CCCCACCTGC TGAGCTCCCC 
AGTTTGAGGC GGAGCACACG CCAAAAGACC ACGGGCTCCT GTGCTAGTGC CAGTCGGCGA 
GGCTCTGGCC TGGGCAAAAG AGGAGCAGCT GAAGCTCGTC GACAGGAGAA GATGGCGGAC 
CCTGAAAGCA GCCAGGAGAC CGTGAATTCT TCAGCTGCAC GGACAGACGA AACTCCCCAA 
GGAGCTGCAG CCTCTAGTTC TGTTGCAGGG GCTGTGGGCA TGACCACCTC TGGGGAGAGT 
GAATCCGATG ATTCTGAGAT GGGACGACTA CAAGCTTTGT TAGAGGCCAG GGGTCTTCCT 
CCTCATCTGT TTGGCCCTCT CGGTCCTCGG ATGTCACAAC TTTTCCATAG AACAATTGGA 
AGCGGAGCTA GCTCTAAGGC CCAGCAGCTT CTGCAAGGAC TACAAGCAAG TGATGAAAGC 
CAGCAACTCC AGGCAGTTAT TGAAATGTGT CAGTTATTGG TTATGGGAAA TGAGGAGACG 
CTGGGAGGGT TTCCGGTCAA GAGTGTCGTT CCAGCTTTGA TAACATTACT GCAGATGGAG 
CATAATTTCG ATATTATGAA CCATGCTTGT CGAGCCTTAA CATATATGAT GGAAGCACTT 
CCTCGGTCAT CTGCTGTTGT AGTAGATGCA ATTCCTGTCT TTTTAGAAAA GCTGCAAGTT 
ATTCAGTGTA TTGATGTCGC AGAGCAGGCC CTGACTGCCT TGGAGATGTT ATCACGCAGA 
CACAGTAAAG CCATTCTACA GGCGGGTGGT TTGGCAGACT GCTTGCTGTA TCTAGAATTC 
TTCAGCATAA ATGCCCAAAG AAATGCACTA GCAATTGCAG CCAATTGCTG CCAGAGTATC 
ACGCCAGATG AATTTCATTT TGTGGCAGAT TCCCTCCCGT TGCTTACCCA AAGGCTAACC 
CATCAGGACA AAAAGTCAGT TGAAAGCACT TGCCTTTGTT TTGCACGTCT AGTGGACAAC 
TTCCAACATG AGGAGAATTT ACTCCAGCAG GTTGCCTCCA AAGATCTGCT TACGAATGTT 
CAACAACTCT TGGTAGTGAC TCCACCCATT TTAAGTTCTG GGATGTTTAT AATGGTGGTT 
CGCATGTTTT CTCTGATGTG TTCCAACTGT CCAACTTTAG CTGTTCAACT TATGAAGCAA 
AACATTGCAG AAACACTTCA CTTTCTCCTG TGTGGTGCCT CTAATGGAAG TTGTCAGGAA 
CAGATTGATC TTGTTCCACG AAGTCCACAA GAACTGTATG AACTGACATC TCTGATTTGC 
GAACTTATGC CATGTTTACC AAAAGAAGGC ATTTTTGCAG TTGATACCAT GCTGAAGAAG 
GGAAATGCGC AGAACACAGA CGGTGCGATT TGGCAGTGGC GTGATGACCG TGGCCTTTGG 
CATCCCTATA ACAGGATTGA CAGCCGGATC ATTGAGGTAG CAGCCCATCA GGTCGGTGAG 
GATGAGATAA GCTTGTCCAC TCTGGGACGA GTTTATACTA TTGATTTTAA TTCTATGCAG 
CAAATCAATG AGGACACGGG AACAGCACGT GCCATTCAGA GAAAACCTAA CCCCTTAGCC 
AATACTAACA CTAGTGGATA TTCAGAGTTA AAGAAGGATG ATGCTCGAGC ACAGCTTATG 
AAAGAGGATC CGGAACTGGC TAAGTCTTTT ATTAAGACAT TATTTGGTGT TCTTTATGAA 
GTGTATAGTT CCTCAGCAGG ACCTGCGGTC AGACATAAGT GCCTTAGAGC AATTCTTAGG 
ATAATTTATT TTGCGGATGC TGAACTTCTG AAGGATGTCC TGAAAAATCA TGCTGTTTCA 
AGTCACATTG CTTCCATGCT GTCAAGCCAA GACCTGAAGA TAGTAGTTGG AGCACTTCAG 
ATGGCAGAAA TCTTAATGCA GAAGTTACCT GATATTTTTA GTGTTTACTT CAGAAGAGAA 
GGTGTAATGC ATCAAGTAAA ACACTTAGCA GAATCAGAGT CTTTGTTGAC AAGTCCACCA 
AAGGCATGTA CGAATGGGTC AGGATCCCTG GGATCCACGA CGTCAGTCAG CAGTGGAACA 
GCCACGGCCG CCACCAATGC TGCAGCTGAC CTGGGGTCCC CCAGCTTGCA GCACAGCAGA 
GATGACTCTT TAGATCTGAG CCCTCAAGGT CGATTAAGTG ATGTTCTAAA GAGAAAACGG 
CTGCCAAAAC GAGGGCCAAG AAGGCCAAAG TATTCACCTC CAAGAGATGA TGACAAAGTA 
GACAATCAAG CTAAAAGCCC TACCACTACT CAGTCACCTA AATCTTCTTT CCTGGCAAGC 
TTGAATCCAA AAACGTGGGG AAGGTTAAGC GCACAGTCCA ACAGCAACAA CATCGAACCA 
GCACGAACTG CGGGAGTCAG TGGTCTTGCC AGGGCCGCCT CCAAGGACAC CATATCCAAT 
AACAGAGAAA AAATTAAAGG TTGGATTAAG GAACAGGCGC ATAAATTTGT AGAGCGTTAT 
TTCAGTTCTG AGAACATGGA TGGAAGCAAC CCTGCACTGA ATGTCCTTCA GAGACTTTGT 
GCTGCAACTG AACAACTCAA CCTCCAGGTG GATGGTGGAG CTGAGTGCCT TGTAGAAATC 
CGTAGCATAG TCTCAGAGTC TGATGTTTCA TCATTTGAAA TCCAACATAG TGGATTTGTG 
AAGCAGCTGT TACTTTATTT GACATCTAAA AGTGAAAAGG ATGCTGTGAG CCGAGAGATC 
AGATTAAAGA GATTCCTTCA CGTATTTTTT TCTTCACCAC TTCCTGGAGA AGAGCCCATT 
GGAAGAGTAG AACCAGTGGG TAATGCGCCT TTGTTGGCAT TAGTTCACAA GATGAACAAC 
TGCCTCAGCC AGATGGAACA GTTTCCAGTC AAAGTACATG ACTTCCCCAG TGGGAACGGG 
ACAGGAGGCA GAGGATCACA GGCTTTAAAA TTTTTCAACA CACACCAGTT AAAATGCCAG 
TTACAAAGAC ATCCAGACTG TGCAAATGTG AAGCAGTGGA AGGGCGGGCC GGTCAAGATT 
GATCCCCTGG CTTTGGTACA AGCTATTGAG AGATACCTTG TAGTTAGAGG GTATGGAAGA 
GTAAGAGAAG ATGATGAAGA CAGTGATGAT GATGGCTCTG ATGAGGAAAT AGACGAGTCT 
CTGGCCGCTC AGTTCTTAAA TTCCGGAAAC GTAAGGCACA GGCTACAGTT TTATATTGGA 
GAACATTTGC TACCATATAA CATGACTGTA TATCAGGCAG TCCGGCAGTT CAGTATACAA 
GCTGAGGATG AGCGGGAGTC CACAGATGAT GAGAGCAACC CTCTGGGAAG AGCTGGTATT 
TGGACAAAGA CTCATACAAT ATGGTACAAA CCTGTGAGAG AGGATGAAGA AAGTAATAAA 
GATTGTGTTG GTGGTAAAAG AGGAAGAGCC CAAACGGCTC CAACAAAAAC ATCCCCTAGA 
AATGCAAAAA AGCATGATGA GTTATGGCAT GATGGAGTAT GCCCATCAGT ATCCAATCCT 
TTAGAAGTTT ACCTCATCTC CTCGCCACCT GAGAATATAA CTTTTGAAGA CCCGTCCTTA 
GATGTGATTC TTCTTCTAAG AGTTTTACAT GCCATCAGCC GATACTGGTA TTACTTGTAT 
GATAATGCAA TGTGCAAGGA GATTATTCCA ACTAGTGAAT TTATTAACAG TAAGTTGACA 
GCAAAAGCAA ATAGGCAGCT TCAAGATCCT TTAGTAATCA TGACAGGAAA TATCCCAACG 
TGGCTTACTG AACTAGGAAA AACCTGCCCA TTTTTCTTCC CTTTTGATAC CCGGCAAATG 
CTTTTTTACG TAACTGCATT TGATCGGGAC CGAGCAATGC AAAGATTACT TGATACCAAC 
CCAGAAATCA ACCAGTCGGA TTCTCAAGAT AGCAGAGTTG CACCTAGATT GGATAGAAAA 
AAACGTACTG TGAACAGAGA GGAGCTGCTG AAACAAGCGG AGTCTGTAAT GCAGGATCTC 
GGTAGCTCAC GGGCCATGTT AGAAATCCAG TATGAAAATG AGGTTGGTAC AGGTCTTGGG 
CCTACACTGG AGTTTTATGC ACTTGTATCT CAGGAACTTC AGAGAGCTGA CTTGGGTCTC 
TGGAGAGGTG AAGAAGTGAC TCTCAGCAAT CCAAAAGGAA GCCAAGAAGG GACCAAGTAT 
ATTCAAAACC TCCAGGGCCT GTTTGCACTT CCCTTTGGTA GGACAGCTAA GCCAGCCCAT 
ATCGCAAAGG TTAAAATGAA GTTTCGCTTC TTAGGAAAAT TAATGGCCAA GGCTATCATG 
GATTTCAGAT TGGTGGACCT TCCCCTTGGT TTACCCTTTT ATAAGTGGAT GCTAAGGCAA 
GAAACTTCAC TGACGTCGCA TGATTTGTTT GACATTGACC CAGTTGTAGC CAGATCAGTG 
TATCACCTAG AAGACATTGT CAGACAGAAG AAAAGACTTG AGCAAGATAA ATCCCAGACC 
AAAGAGAGTC TACAATATGC ATTAGAAACC CTGACTATGA ATGGCTGCTC AGTTGAAGAT 
CTAGGATTGG ATTTCACGCT GCCAGGGTTT CCCAACATTG AACTGAAGAA AGGAGGAAAG 
GATATACCAG TTACCATCCA CAATTTAGAG GAGTATCTAA GACTGGTTAT ATTCTGGGCA 
CTAAATGAAG GCGTTTCCAG GCAATTTGAT TCCTTCAGAG ATGGATTTGA ATCTGTCTTC 
CCACTTAGTC ATCTTCAGTA CTTCTACCCG GAAGAACTGG ATCAGCTCTT GTGCGGCAGT 
AAAGCAGACA CGTGGGATGC GAAGACACTG ATGGAGTGCT GCAGGCCGGA CCACGGTTAT 
ACTCATGACA GTCGGGCTGT GAAGTTCTTG TTTGAGATTC TCAGTAGTTT TGATAATGAG 
CAGCAGAGGT TGTTTCTCCA GTTTGTGACA GGTAGCCCAA GATTGCCTGT TGGAGGCTTC 
CGGAGTCTGA ATCCACCTCT GACAATTGTC CGGAAGACCT TCGAGTCCAC GGAGAACCCC 
GACGACTTCT TGCCCTCTGT AATGACTTGT GTGAACTACC TTAAGTTGCC GGACTACTCG 
AGCATTGACA TCATGCGTGA GAAACTGTTG ATTGCAGCGA GAGAAGGGCA GCAGTCATTC 
CATCTTTCCT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TRIP12 ( XM_022409665.1 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_022265373.1 Canis lupus familiaris thyroid hormone receptor interactor 12 (TRIP12), transcript variant X5, mRNA.

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CloneID	OCe25763
Clone ID Related Accession (Same CDS sequence)	XM_005635803.2 , XM_022409665.1
Accession Version	XM_022409665.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6192bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-09-04
Organism	Canis lupus familiaris(dog)
Product	E3 ubiquitin-protein ligase TRIP12 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006607.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCCAACC GGCCTAATAA CAATCCAGGG GGGTCACTGC GACGTTCACA GAGGAACACT 
GCCGGGGCCC AACCACAAGA CGACTCGATA GGAGGAAGGT CACATTTAGG GCAGGCAAAA 
CATAAGGGAT ATAGCCCTCC TGAGAGTAGA AAATCTAATT CTAAGGCACC CAAAGTGCAG 
TCTAATACTA CTTCTGAACT GTCAAGGGGA CACCTTTCTA AAAGAAGCTG CAGTTCATCA 
TCTGCTGTCA TAGTTCCACA ACCAGAGGAT CCAGACAGAG CCAATACTTC AGAAAGGCAA 
AAAACGGGGC AGGTGCCTAA GAAAGACAAT TCTCGAGGAG TGAAACGCAG TGCTAGTCCA 
GACTACAACA GGACCAATTC TCCTAGCTCT GCCAAAAAAC CAAAAGCACT TCAGCATACT 
GAATCTCCCT CAGAAACAAA TAAGCCACAT AGTAAGTCGA AGAAGAGACA TTTAGACCAG 
GAGCAACAAC TGAAATCTGC ACAGTCGCCA TCAACAAGTA AGGCTCACAC CAGAAAGAGT 
GGGGCCACCG GCGGCTCACG GAGTCAGAAA AGAAAGAGGA CAGAGAGTTC TTGTCTAAAG 
AGTGGTTCTG TGTCTGAGTC AACTGGTGCC GAAGAGAGAT CTGCAAAACC CACCAAGCTG 
GCTTCAAAAT CCGCCGCCTC GGCCAAAGCT GGGTGCAGCA CCATCACTGA TTCTTCCTCC 
GCTGCCTCTA CTTCCTCCTC ATCTTCTGCT GTCGCCTCGG CCTCCTCCAC TGTACCACCA 
GGGGCCAGGG TGAAACAAGG AAAAGACCAG AGCAAGGCCA GGCGTTCCCG TTCAGCATCC 
AGTCCCAGTC CCAGAAGAAG TAGCAGGGAA AAGGAACAGA GTAAAACTGG TGGCTCTTCA 
AAATTCGATT GGGCTGCTCG TTTCAGCCCT AAAGTTAGCC TTCCTAAAAC CAAACTGTCT 
CTTCCAGGGT CTTCTAAGTC AGAGACATCA AAACCTGGAC CTTCTGGATT ACAGGCCAAA 
TTAGCAAGTT TAAGAAAATC TACCAAGAAG CGCAGTGAAT CCCCACCTGC TGAGCTCCCC 
AGTTTGAGGC GGAGCACACG CCAAAAGACC ACGGGCTCCT GTGCTAGTGC CAGTCGGCGA 
GGCTCTGGCC TGGGCAAAAG AGGAGCAGCT GAAGCTCGTC GACAGGAGAA GATGGCGGAC 
CCTGAAAGCA GCCAGGAGAC CGTGAATTCT TCAGCTGCAC GGACAGACGA AACTCCCCAA 
GGAGCTGCAG CCTCTAGTTC TGTTGCAGGG GCTGTGGGCA TGACCACCTC TGGGGAGAGT 
GAATCCGATG ATTCTGAGAT GGGACGACTA CAAGCTTTGT TAGAGGCCAG GGGTCTTCCT 
CCTCATCTGT TTGGCCCTCT CGGTCCTCGG ATGTCACAAC TTTTCCATAG AACAATTGGA 
AGCGGAGCTA GCTCTAAGGC CCAGCAGCTT CTGCAAGGAC TACAAGCAAG TGATGAAAGC 
CAGCAACTCC AGGCAGTTAT TGAAATGTGT CAGTTATTGG TTATGGGAAA TGAGGAGACG 
CTGGGAGGGT TTCCGGTCAA GAGTGTCGTT CCAGCTTTGA TAACATTACT GCAGATGGAG 
CATAATTTCG ATATTATGAA CCATGCTTGT CGAGCCTTAA CATATATGAT GGAAGCACTT 
CCTCGGTCAT CTGCTGTTGT AGTAGATGCA ATTCCTGTCT TTTTAGAAAA GCTGCAAGTT 
ATTCAGTGTA TTGATGTCGC AGAGCAGGCC CTGACTGCCT TGGAGATGTT ATCACGCAGA 
CACAGTAAAG CCATTCTACA GGCGGGTGGT TTGGCAGACT GCTTGCTGTA TCTAGAATTC 
TTCAGCATAA ATGCCCAAAG AAATGCACTA GCAATTGCAG CCAATTGCTG CCAGAGTATC 
ACGCCAGATG AATTTCATTT TGTGGCAGAT TCCCTCCCGT TGCTTACCCA AAGGCTAACC 
CATCAGGACA AAAAGTCAGT TGAAAGCACT TGCCTTTGTT TTGCACGTCT AGTGGACAAC 
TTCCAACATG AGGAGAATTT ACTCCAGCAG GTTGCCTCCA AAGATCTGCT TACGAATGTT 
CAACAACTCT TGGTAGTGAC TCCACCCATT TTAAGTTCTG GGATGTTTAT AATGGTGGTT 
CGCATGTTTT CTCTGATGTG TTCCAACTGT CCAACTTTAG CTGTTCAACT TATGAAGCAA 
AACATTGCAG AAACACTTCA CTTTCTCCTG TGTGGTGCCT CTAATGGAAG TTGTCAGGAA 
CAGATTGATC TTGTTCCACG AAGTCCACAA GAACTGTATG AACTGACATC TCTGATTTGC 
GAACTTATGC CATGTTTACC AAAAGAAGGC ATTTTTGCAG TTGATACCAT GCTGAAGAAG 
GGAAATGCGC AGAACACAGA CGGTGCGATT TGGCAGTGGC GTGATGACCG TGGCCTTTGG 
CATCCCTATA ACAGGATTGA CAGCCGGATC ATTGAGGTAG CAGCCCATCA GGTCGGTGAG 
GATGAGATAA GCTTGTCCAC TCTGGGACGA GTTTATACTA TTGATTTTAA TTCTATGCAG 
CAAATCAATG AGGACACGGG AACAGCACGT GCCATTCAGA GAAAACCTAA CCCCTTAGCC 
AATACTAACA CTAGTGGATA TTCAGAGTTA AAGAAGGATG ATGCTCGAGC ACAGCTTATG 
AAAGAGGATC CGGAACTGGC TAAGTCTTTT ATTAAGACAT TATTTGGTGT TCTTTATGAA 
GTGTATAGTT CCTCAGCAGG ACCTGCGGTC AGACATAAGT GCCTTAGAGC AATTCTTAGG 
ATAATTTATT TTGCGGATGC TGAACTTCTG AAGGATGTCC TGAAAAATCA TGCTGTTTCA 
AGTCACATTG CTTCCATGCT GTCAAGCCAA GACCTGAAGA TAGTAGTTGG AGCACTTCAG 
ATGGCAGAAA TCTTAATGCA GAAGTTACCT GATATTTTTA GTGTTTACTT CAGAAGAGAA 
GGTGTAATGC ATCAAGTAAA ACACTTAGCA GAATCAGAGT CTTTGTTGAC AAGTCCACCA 
AAGGCATGTA CGAATGGGTC AGGATCCCTG GGATCCACGA CGTCAGTCAG CAGTGGAACA 
GCCACGGCCG CCACCAATGC TGCAGCTGAC CTGGGGTCCC CCAGCTTGCA GCACAGCAGA 
GATGACTCTT TAGATCTGAG CCCTCAAGGT CGATTAAGTG ATGTTCTAAA GAGAAAACGG 
CTGCCAAAAC GAGGGCCAAG AAGGCCAAAG TATTCACCTC CAAGAGATGA TGACAAAGTA 
GACAATCAAG CTAAAAGCCC TACCACTACT CAGTCACCTA AATCTTCTTT CCTGGCAAGC 
TTGAATCCAA AAACGTGGGG AAGGTTAAGC GCACAGTCCA ACAGCAACAA CATCGAACCA 
GCACGAACTG CGGGAGTCAG TGGTCTTGCC AGGGCCGCCT CCAAGGACAC CATATCCAAT 
AACAGAGAAA AAATTAAAGG TTGGATTAAG GAACAGGCGC ATAAATTTGT AGAGCGTTAT 
TTCAGTTCTG AGAACATGGA TGGAAGCAAC CCTGCACTGA ATGTCCTTCA GAGACTTTGT 
GCTGCAACTG AACAACTCAA CCTCCAGGTG GATGGTGGAG CTGAGTGCCT TGTAGAAATC 
CGTAGCATAG TCTCAGAGTC TGATGTTTCA TCATTTGAAA TCCAACATAG TGGATTTGTG 
AAGCAGCTGT TACTTTATTT GACATCTAAA AGTGAAAAGG ATGCTGTGAG CCGAGAGATC 
AGATTAAAGA GATTCCTTCA CGTATTTTTT TCTTCACCAC TTCCTGGAGA AGAGCCCATT 
GGAAGAGTAG AACCAGTGGG TAATGCGCCT TTGTTGGCAT TAGTTCACAA GATGAACAAC 
TGCCTCAGCC AGATGGAACA GTTTCCAGTC AAAGTACATG ACTTCCCCAG TGGGAACGGG 
ACAGGAGGCA GAGGATCACA GGCTTTAAAA TTTTTCAACA CACACCAGTT AAAATGCCAG 
TTACAAAGAC ATCCAGACTG TGCAAATGTG AAGCAGTGGA AGGGCGGGCC GGTCAAGATT 
GATCCCCTGG CTTTGGTACA AGCTATTGAG AGATACCTTG TAGTTAGAGG GTATGGAAGA 
GTAAGAGAAG ATGATGAAGA CAGTGATGAT GATGGCTCTG ATGAGGAAAT AGACGAGTCT 
CTGGCCGCTC AGTTCTTAAA TTCCGGAAAC GTAAGGCACA GGCTACAGTT TTATATTGGA 
GAACATTTGC TACCATATAA CATGACTGTA TATCAGGCAG TCCGGCAGTT CAGTATACAA 
GCTGAGGATG AGCGGGAGTC CACAGATGAT GAGAGCAACC CTCTGGGAAG AGCTGGTATT 
TGGACAAAGA CTCATACAAT ATGGTACAAA CCTGTGAGAG AGGATGAAGA AAGTAATAAA 
GATTGTGTTG GTGGTAAAAG AGGAAGAGCC CAAACGGCTC CAACAAAAAC ATCCCCTAGA 
AATGCAAAAA AGCATGATGA GTTATGGCAT GATGGAGTAT GCCCATCAGT ATCCAATCCT 
TTAGAAGTTT ACCTCATCTC CTCGCCACCT GAGAATATAA CTTTTGAAGA CCCGTCCTTA 
GATGTGATTC TTCTTCTAAG AGTTTTACAT GCCATCAGCC GATACTGGTA TTACTTGTAT 
GATAATGCAA TGTGCAAGGA GATTATTCCA ACTAGTGAAT TTATTAACAG TAAGTTGACA 
GCAAAAGCAA ATAGGCAGCT TCAAGATCCT TTAGTAATCA TGACAGGAAA TATCCCAACG 
TGGCTTACTG AACTAGGAAA AACCTGCCCA TTTTTCTTCC CTTTTGATAC CCGGCAAATG 
CTTTTTTACG TAACTGCATT TGATCGGGAC CGAGCAATGC AAAGATTACT TGATACCAAC 
CCAGAAATCA ACCAGTCGGA TTCTCAAGAT AGCAGAGTTG CACCTAGATT GGATAGAAAA 
AAACGTACTG TGAACAGAGA GGAGCTGCTG AAACAAGCGG AGTCTGTAAT GCAGGATCTC 
GGTAGCTCAC GGGCCATGTT AGAAATCCAG TATGAAAATG AGGTTGGTAC AGGTCTTGGG 
CCTACACTGG AGTTTTATGC ACTTGTATCT CAGGAACTTC AGAGAGCTGA CTTGGGTCTC 
TGGAGAGGTG AAGAAGTGAC TCTCAGCAAT CCAAAAGGAA GCCAAGAAGG GACCAAGTAT 
ATTCAAAACC TCCAGGGCCT GTTTGCACTT CCCTTTGGTA GGACAGCTAA GCCAGCCCAT 
ATCGCAAAGG TTAAAATGAA GTTTCGCTTC TTAGGAAAAT TAATGGCCAA GGCTATCATG 
GATTTCAGAT TGGTGGACCT TCCCCTTGGT TTACCCTTTT ATAAGTGGAT GCTAAGGCAA 
GAAACTTCAC TGACGTCGCA TGATTTGTTT GACATTGACC CAGTTGTAGC CAGATCAGTG 
TATCACCTAG AAGACATTGT CAGACAGAAG AAAAGACTTG AGCAAGATAA ATCCCAGACC 
AAAGAGAGTC TACAATATGC ATTAGAAACC CTGACTATGA ATGGCTGCTC AGTTGAAGAT 
CTAGGATTGG ATTTCACGCT GCCAGGGTTT CCCAACATTG AACTGAAGAA AGGAGGAAAG 
GATATACCAG TTACCATCCA CAATTTAGAG GAGTATCTAA GACTGGTTAT ATTCTGGGCA 
CTAAATGAAG GCGTTTCCAG GCAATTTGAT TCCTTCAGAG ATGGATTTGA ATCTGTCTTC 
CCACTTAGTC ATCTTCAGTA CTTCTACCCG GAAGAACTGG ATCAGCTCTT GTGCGGCAGT 
AAAGCAGACA CGTGGGATGC GAAGACACTG ATGGAGTGCT GCAGGCCGGA CCACGGTTAT 
ACTCATGACA GTCGGGCTGT GAAGTTCTTG TTTGAGATTC TCAGTAGTTT TGATAATGAG 
CAGCAGAGGT TGTTTCTCCA GTTTGTGACA GGTAGCCCAA GATTGCCTGT TGGAGGCTTC 
CGGAGTCTGA ATCCACCTCT GACAATTGTC CGGAAGACCT TCGAGTCCAC GGAGAACCCC 
GACGACTTCT TGCCCTCTGT AATGACTTGT GTGAACTACC TTAAGTTGCC GGACTACTCG 
AGCATTGACA TCATGCGTGA GAAACTGTTG ATTGCAGCGA GAGAAGGGCA GCAGTCATTC 
CATCTTTCCT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_022265373.1
CDS	1..6192
Translation	MSNRPNNNPGGSLRRSQRNTAGAQPQDDSIGGRSHLGQAKHKGYSPPESRKSNSKAPKVQSNTTSELSRGHLSKRSCSSSSAVIVPQPEDPDRANTSERQKTGQVPKKDNSRGVKRSASPDYNRTNSPSSAKKPKALQHTESPSETNKPHSKSKKRHLDQEQQLKSAQSPSTSKAHTRKSGATGGSRSQKRKRTESSCLKSGSVSESTGAEERSAKPTKLASKSAASAKAGCSTITDSSSAASTSSSSSAVASASSTVPPGARVKQGKDQSKARRSRSASSPSPRRSSREKEQSKTGGSSKFDWAARFSPKVSLPKTKLSLPGSSKSETSKPGPSGLQAKLASLRKSTKKRSESPPAELPSLRRSTRQKTTGSCASASRRGSGLGKRGAAEARRQEKMADPESSQETVNSSAARTDETPQGAAASSSVAGAVGMTTSGESESDDSEMGRLQALLEARGLPPHLFGPLGPRMSQLFHRTIGSGASSKAQQLLQGLQASDESQQLQAVIEMCQLLVMGNEETLGGFPVKSVVPALITLLQMEHNFDIMNHACRALTYMMEALPRSSAVVVDAIPVFLEKLQVIQCIDVAEQALTALEMLSRRHSKAILQAGGLADCLLYLEFFSINAQRNALAIAANCCQSITPDEFHFVADSLPLLTQRLTHQDKKSVESTCLCFARLVDNFQHEENLLQQVASKDLLTNVQQLLVVTPPILSSGMFIMVVRMFSLMCSNCPTLAVQLMKQNIAETLHFLLCGASNGSCQEQIDLVPRSPQELYELTSLICELMPCLPKEGIFAVDTMLKKGNAQNTDGAIWQWRDDRGLWHPYNRIDSRIIEVAAHQVGEDEISLSTLGRVYTIDFNSMQQINEDTGTARAIQRKPNPLANTNTSGYSELKKDDARAQLMKEDPELAKSFIKTLFGVLYEVYSSSAGPAVRHKCLRAILRIIYFADAELLKDVLKNHAVSSHIASMLSSQDLKIVVGALQMAEILMQKLPDIFSVYFRREGVMHQVKHLAESESLLTSPPKACTNGSGSLGSTTSVSSGTATAATNAAADLGSPSLQHSRDDSLDLSPQGRLSDVLKRKRLPKRGPRRPKYSPPRDDDKVDNQAKSPTTTQSPKSSFLASLNPKTWGRLSAQSNSNNIEPARTAGVSGLARAASKDTISNNREKIKGWIKEQAHKFVERYFSSENMDGSNPALNVLQRLCAATEQLNLQVDGGAECLVEIRSIVSESDVSSFEIQHSGFVKQLLLYLTSKSEKDAVSREIRLKRFLHVFFSSPLPGEEPIGRVEPVGNAPLLALVHKMNNCLSQMEQFPVKVHDFPSGNGTGGRGSQALKFFNTHQLKCQLQRHPDCANVKQWKGGPVKIDPLALVQAIERYLVVRGYGRVREDDEDSDDDGSDEEIDESLAAQFLNSGNVRHRLQFYIGEHLLPYNMTVYQAVRQFSIQAEDERESTDDESNPLGRAGIWTKTHTIWYKPVREDEESNKDCVGGKRGRAQTAPTKTSPRNAKKHDELWHDGVCPSVSNPLEVYLISSPPENITFEDPSLDVILLLRVLHAISRYWYYLYDNAMCKEIIPTSEFINSKLTAKANRQLQDPLVIMTGNIPTWLTELGKTCPFFFPFDTRQMLFYVTAFDRDRAMQRLLDTNPEINQSDSQDSRVAPRLDRKKRTVNREELLKQAESVMQDLGSSRAMLEIQYENEVGTGLGPTLEFYALVSQELQRADLGLWRGEEVTLSNPKGSQEGTKYIQNLQGLFALPFGRTAKPAHIAKVKMKFRFLGKLMAKAIMDFRLVDLPLGLPFYKWMLRQETSLTSHDLFDIDPVVARSVYHLEDIVRQKKRLEQDKSQTKESLQYALETLTMNGCSVEDLGLDFTLPGFPNIELKKGGKDIPVTIHNLEEYLRLVIFWALNEGVSRQFDSFRDGFESVFPLSHLQYFYPEELDQLLCGSKADTWDAKTLMECCRPDHGYTHDSRAVKFLFEILSSFDNEQQRLFLQFVTGSPRLPVGGFRSLNPPLTIVRKTFESTENPDDFLPSVMTCVNYLKLPDYSSIDIMREKLLIAAREGQQSFHLS

Target ORF information:

RefSeq Version	XM_022409665.1
Organism	Canis lupus familiaris(dog)
Definition	Canis lupus familiaris thyroid hormone receptor interactor 12 (TRIP12), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_022409665.1

ORF Insert Sequence:

ATGTCCAACC GGCCTAATAA CAATCCAGGG GGGTCACTGC GACGTTCACA GAGGAACACT 
GCCGGGGCCC AACCACAAGA CGACTCGATA GGAGGAAGGT CACATTTAGG GCAGGCAAAA 
CATAAGGGAT ATAGCCCTCC TGAGAGTAGA AAATCTAATT CTAAGGCACC CAAAGTGCAG 
TCTAATACTA CTTCTGAACT GTCAAGGGGA CACCTTTCTA AAAGAAGCTG CAGTTCATCA 
TCTGCTGTCA TAGTTCCACA ACCAGAGGAT CCAGACAGAG CCAATACTTC AGAAAGGCAA 
AAAACGGGGC AGGTGCCTAA GAAAGACAAT TCTCGAGGAG TGAAACGCAG TGCTAGTCCA 
GACTACAACA GGACCAATTC TCCTAGCTCT GCCAAAAAAC CAAAAGCACT TCAGCATACT 
GAATCTCCCT CAGAAACAAA TAAGCCACAT AGTAAGTCGA AGAAGAGACA TTTAGACCAG 
GAGCAACAAC TGAAATCTGC ACAGTCGCCA TCAACAAGTA AGGCTCACAC CAGAAAGAGT 
GGGGCCACCG GCGGCTCACG GAGTCAGAAA AGAAAGAGGA CAGAGAGTTC TTGTCTAAAG 
AGTGGTTCTG TGTCTGAGTC AACTGGTGCC GAAGAGAGAT CTGCAAAACC CACCAAGCTG 
GCTTCAAAAT CCGCCGCCTC GGCCAAAGCT GGGTGCAGCA CCATCACTGA TTCTTCCTCC 
GCTGCCTCTA CTTCCTCCTC ATCTTCTGCT GTCGCCTCGG CCTCCTCCAC TGTACCACCA 
GGGGCCAGGG TGAAACAAGG AAAAGACCAG AGCAAGGCCA GGCGTTCCCG TTCAGCATCC 
AGTCCCAGTC CCAGAAGAAG TAGCAGGGAA AAGGAACAGA GTAAAACTGG TGGCTCTTCA 
AAATTCGATT GGGCTGCTCG TTTCAGCCCT AAAGTTAGCC TTCCTAAAAC CAAACTGTCT 
CTTCCAGGGT CTTCTAAGTC AGAGACATCA AAACCTGGAC CTTCTGGATT ACAGGCCAAA 
TTAGCAAGTT TAAGAAAATC TACCAAGAAG CGCAGTGAAT CCCCACCTGC TGAGCTCCCC 
AGTTTGAGGC GGAGCACACG CCAAAAGACC ACGGGCTCCT GTGCTAGTGC CAGTCGGCGA 
GGCTCTGGCC TGGGCAAAAG AGGAGCAGCT GAAGCTCGTC GACAGGAGAA GATGGCGGAC 
CCTGAAAGCA GCCAGGAGAC CGTGAATTCT TCAGCTGCAC GGACAGACGA AACTCCCCAA 
GGAGCTGCAG CCTCTAGTTC TGTTGCAGGG GCTGTGGGCA TGACCACCTC TGGGGAGAGT 
GAATCCGATG ATTCTGAGAT GGGACGACTA CAAGCTTTGT TAGAGGCCAG GGGTCTTCCT 
CCTCATCTGT TTGGCCCTCT CGGTCCTCGG ATGTCACAAC TTTTCCATAG AACAATTGGA 
AGCGGAGCTA GCTCTAAGGC CCAGCAGCTT CTGCAAGGAC TACAAGCAAG TGATGAAAGC 
CAGCAACTCC AGGCAGTTAT TGAAATGTGT CAGTTATTGG TTATGGGAAA TGAGGAGACG 
CTGGGAGGGT TTCCGGTCAA GAGTGTCGTT CCAGCTTTGA TAACATTACT GCAGATGGAG 
CATAATTTCG ATATTATGAA CCATGCTTGT CGAGCCTTAA CATATATGAT GGAAGCACTT 
CCTCGGTCAT CTGCTGTTGT AGTAGATGCA ATTCCTGTCT TTTTAGAAAA GCTGCAAGTT 
ATTCAGTGTA TTGATGTCGC AGAGCAGGCC CTGACTGCCT TGGAGATGTT ATCACGCAGA 
CACAGTAAAG CCATTCTACA GGCGGGTGGT TTGGCAGACT GCTTGCTGTA TCTAGAATTC 
TTCAGCATAA ATGCCCAAAG AAATGCACTA GCAATTGCAG CCAATTGCTG CCAGAGTATC 
ACGCCAGATG AATTTCATTT TGTGGCAGAT TCCCTCCCGT TGCTTACCCA AAGGCTAACC 
CATCAGGACA AAAAGTCAGT TGAAAGCACT TGCCTTTGTT TTGCACGTCT AGTGGACAAC 
TTCCAACATG AGGAGAATTT ACTCCAGCAG GTTGCCTCCA AAGATCTGCT TACGAATGTT 
CAACAACTCT TGGTAGTGAC TCCACCCATT TTAAGTTCTG GGATGTTTAT AATGGTGGTT 
CGCATGTTTT CTCTGATGTG TTCCAACTGT CCAACTTTAG CTGTTCAACT TATGAAGCAA 
AACATTGCAG AAACACTTCA CTTTCTCCTG TGTGGTGCCT CTAATGGAAG TTGTCAGGAA 
CAGATTGATC TTGTTCCACG AAGTCCACAA GAACTGTATG AACTGACATC TCTGATTTGC 
GAACTTATGC CATGTTTACC AAAAGAAGGC ATTTTTGCAG TTGATACCAT GCTGAAGAAG 
GGAAATGCGC AGAACACAGA CGGTGCGATT TGGCAGTGGC GTGATGACCG TGGCCTTTGG 
CATCCCTATA ACAGGATTGA CAGCCGGATC ATTGAGGTAG CAGCCCATCA GGTCGGTGAG 
GATGAGATAA GCTTGTCCAC TCTGGGACGA GTTTATACTA TTGATTTTAA TTCTATGCAG 
CAAATCAATG AGGACACGGG AACAGCACGT GCCATTCAGA GAAAACCTAA CCCCTTAGCC 
AATACTAACA CTAGTGGATA TTCAGAGTTA AAGAAGGATG ATGCTCGAGC ACAGCTTATG 
AAAGAGGATC CGGAACTGGC TAAGTCTTTT ATTAAGACAT TATTTGGTGT TCTTTATGAA 
GTGTATAGTT CCTCAGCAGG ACCTGCGGTC AGACATAAGT GCCTTAGAGC AATTCTTAGG 
ATAATTTATT TTGCGGATGC TGAACTTCTG AAGGATGTCC TGAAAAATCA TGCTGTTTCA 
AGTCACATTG CTTCCATGCT GTCAAGCCAA GACCTGAAGA TAGTAGTTGG AGCACTTCAG 
ATGGCAGAAA TCTTAATGCA GAAGTTACCT GATATTTTTA GTGTTTACTT CAGAAGAGAA 
GGTGTAATGC ATCAAGTAAA ACACTTAGCA GAATCAGAGT CTTTGTTGAC AAGTCCACCA 
AAGGCATGTA CGAATGGGTC AGGATCCCTG GGATCCACGA CGTCAGTCAG CAGTGGAACA 
GCCACGGCCG CCACCAATGC TGCAGCTGAC CTGGGGTCCC CCAGCTTGCA GCACAGCAGA 
GATGACTCTT TAGATCTGAG CCCTCAAGGT CGATTAAGTG ATGTTCTAAA GAGAAAACGG 
CTGCCAAAAC GAGGGCCAAG AAGGCCAAAG TATTCACCTC CAAGAGATGA TGACAAAGTA 
GACAATCAAG CTAAAAGCCC TACCACTACT CAGTCACCTA AATCTTCTTT CCTGGCAAGC 
TTGAATCCAA AAACGTGGGG AAGGTTAAGC GCACAGTCCA ACAGCAACAA CATCGAACCA 
GCACGAACTG CGGGAGTCAG TGGTCTTGCC AGGGCCGCCT CCAAGGACAC CATATCCAAT 
AACAGAGAAA AAATTAAAGG TTGGATTAAG GAACAGGCGC ATAAATTTGT AGAGCGTTAT 
TTCAGTTCTG AGAACATGGA TGGAAGCAAC CCTGCACTGA ATGTCCTTCA GAGACTTTGT 
GCTGCAACTG AACAACTCAA CCTCCAGGTG GATGGTGGAG CTGAGTGCCT TGTAGAAATC 
CGTAGCATAG TCTCAGAGTC TGATGTTTCA TCATTTGAAA TCCAACATAG TGGATTTGTG 
AAGCAGCTGT TACTTTATTT GACATCTAAA AGTGAAAAGG ATGCTGTGAG CCGAGAGATC 
AGATTAAAGA GATTCCTTCA CGTATTTTTT TCTTCACCAC TTCCTGGAGA AGAGCCCATT 
GGAAGAGTAG AACCAGTGGG TAATGCGCCT TTGTTGGCAT TAGTTCACAA GATGAACAAC 
TGCCTCAGCC AGATGGAACA GTTTCCAGTC AAAGTACATG ACTTCCCCAG TGGGAACGGG 
ACAGGAGGCA GAGGATCACA GGCTTTAAAA TTTTTCAACA CACACCAGTT AAAATGCCAG 
TTACAAAGAC ATCCAGACTG TGCAAATGTG AAGCAGTGGA AGGGCGGGCC GGTCAAGATT 
GATCCCCTGG CTTTGGTACA AGCTATTGAG AGATACCTTG TAGTTAGAGG GTATGGAAGA 
GTAAGAGAAG ATGATGAAGA CAGTGATGAT GATGGCTCTG ATGAGGAAAT AGACGAGTCT 
CTGGCCGCTC AGTTCTTAAA TTCCGGAAAC GTAAGGCACA GGCTACAGTT TTATATTGGA 
GAACATTTGC TACCATATAA CATGACTGTA TATCAGGCAG TCCGGCAGTT CAGTATACAA 
GCTGAGGATG AGCGGGAGTC CACAGATGAT GAGAGCAACC CTCTGGGAAG AGCTGGTATT 
TGGACAAAGA CTCATACAAT ATGGTACAAA CCTGTGAGAG AGGATGAAGA AAGTAATAAA 
GATTGTGTTG GTGGTAAAAG AGGAAGAGCC CAAACGGCTC CAACAAAAAC ATCCCCTAGA 
AATGCAAAAA AGCATGATGA GTTATGGCAT GATGGAGTAT GCCCATCAGT ATCCAATCCT 
TTAGAAGTTT ACCTCATCTC CTCGCCACCT GAGAATATAA CTTTTGAAGA CCCGTCCTTA 
GATGTGATTC TTCTTCTAAG AGTTTTACAT GCCATCAGCC GATACTGGTA TTACTTGTAT 
GATAATGCAA TGTGCAAGGA GATTATTCCA ACTAGTGAAT TTATTAACAG TAAGTTGACA 
GCAAAAGCAA ATAGGCAGCT TCAAGATCCT TTAGTAATCA TGACAGGAAA TATCCCAACG 
TGGCTTACTG AACTAGGAAA AACCTGCCCA TTTTTCTTCC CTTTTGATAC CCGGCAAATG 
CTTTTTTACG TAACTGCATT TGATCGGGAC CGAGCAATGC AAAGATTACT TGATACCAAC 
CCAGAAATCA ACCAGTCGGA TTCTCAAGAT AGCAGAGTTG CACCTAGATT GGATAGAAAA 
AAACGTACTG TGAACAGAGA GGAGCTGCTG AAACAAGCGG AGTCTGTAAT GCAGGATCTC 
GGTAGCTCAC GGGCCATGTT AGAAATCCAG TATGAAAATG AGGTTGGTAC AGGTCTTGGG 
CCTACACTGG AGTTTTATGC ACTTGTATCT CAGGAACTTC AGAGAGCTGA CTTGGGTCTC 
TGGAGAGGTG AAGAAGTGAC TCTCAGCAAT CCAAAAGGAA GCCAAGAAGG GACCAAGTAT 
ATTCAAAACC TCCAGGGCCT GTTTGCACTT CCCTTTGGTA GGACAGCTAA GCCAGCCCAT 
ATCGCAAAGG TTAAAATGAA GTTTCGCTTC TTAGGAAAAT TAATGGCCAA GGCTATCATG 
GATTTCAGAT TGGTGGACCT TCCCCTTGGT TTACCCTTTT ATAAGTGGAT GCTAAGGCAA 
GAAACTTCAC TGACGTCGCA TGATTTGTTT GACATTGACC CAGTTGTAGC CAGATCAGTG 
TATCACCTAG AAGACATTGT CAGACAGAAG AAAAGACTTG AGCAAGATAA ATCCCAGACC 
AAAGAGAGTC TACAATATGC ATTAGAAACC CTGACTATGA ATGGCTGCTC AGTTGAAGAT 
CTAGGATTGG ATTTCACGCT GCCAGGGTTT CCCAACATTG AACTGAAGAA AGGAGGAAAG 
GATATACCAG TTACCATCCA CAATTTAGAG GAGTATCTAA GACTGGTTAT ATTCTGGGCA 
CTAAATGAAG GCGTTTCCAG GCAATTTGAT TCCTTCAGAG ATGGATTTGA ATCTGTCTTC 
CCACTTAGTC ATCTTCAGTA CTTCTACCCG GAAGAACTGG ATCAGCTCTT GTGCGGCAGT 
AAAGCAGACA CGTGGGATGC GAAGACACTG ATGGAGTGCT GCAGGCCGGA CCACGGTTAT 
ACTCATGACA GTCGGGCTGT GAAGTTCTTG TTTGAGATTC TCAGTAGTTT TGATAATGAG 
CAGCAGAGGT TGTTTCTCCA GTTTGTGACA GGTAGCCCAA GATTGCCTGT TGGAGGCTTC 
CGGAGTCTGA ATCCACCTCT GACAATTGTC CGGAAGACCT TCGAGTCCAC GGAGAACCCC 
GACGACTTCT TGCCCTCTGT AATGACTTGT GTGAACTACC TTAAGTTGCC GGACTACTCG 
AGCATTGACA TCATGCGTGA GAAACTGTTG ATTGCAGCGA GAGAAGGGCA GCAGTCATTC 
CATCTTTCCT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TRIP12 ( XM_005635804.2 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_005635861.1 Canis lupus familiaris thyroid hormone receptor interactor 12 (TRIP12), transcript variant X5, mRNA.

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CloneID	OCe25764
Clone ID Related Accession (Same CDS sequence)	XM_005635804.2 , XM_022409667.1
Accession Version	XM_005635804.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6126bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-09-16
Organism	Canis lupus familiaris(Dog)
Product	E3 ubiquitin-protein ligase TRIP12 isoform X4
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726103.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 17, 2015 this sequence version replaced XM_005635804.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCCAACC GGCCTAATAA CAATCCAGGG GGGTCACTGC GACGTTCACA GAGGAACACT 
GCCGGGGCCC AACCACAAGA CGACTCGATA GGAGGAAGGT CACATTTAGG GCAGGCAAAA 
CATAAGGGAT ATAGCCCTCC TGAGAGTAGA AAATCTAATT CTAAGGCACC CAAAGTGCAG 
TCTAATACTA CTTCTGAACT GTCAAGGGGA CACCTTTCTA AAAGAAGCTG CAGTTCATCA 
TCTGCTGTCA TAGTTCCACA ACCAGAGGAT CCAGACAGAG CCAATACTTC AGAAAGGCAA 
AAAACGGGGC AGGTGCCTAA GAAAGACAAT TCTCGAGGAG TGAAACGCAG TGCTAGTCCA 
GACTACAACA GGACCAATTC TCCTAGCTCT GCCAAAAAAC CAAAAGCACT TCAGCATACT 
GAATCTCCCT CAGAAACAAA TAAGCCACAT AGTAAGTCGA AGAAGAGACA TTTAGACCAG 
GAGCAACAAC TGAAATCTGC ACAGTCGCCA TCAACAAGTA AGGCTCACAC CAGAAAGAGT 
GGGGCCACCG GCGGCTCACG GAGTCAGAAA AGAAAGAGGA CAGAGAGTTC TTGTCTAAAG 
AGTGGTTCTG TGTCTGAGTC AACTGGTGCC GAAGAGAGAT CTGCAAAACC CACCAAGCTG 
GCTTCAAAAT CCGCCGCCTC GGCCAAAGCT GGGTGCAGCA CCATCACTGA TTCTTCCTCC 
GCTGCCTCTA CTTCCTCCTC ATCTTCTGCT GTCGCCTCGG CCTCCTCCAC TGTACCACCA 
GGGGCCAGGG TGAAACAAGG AAAAGACCAG AGCAAGGCCA GGCGTTCCCG TTCAGCATCC 
AGTCCCAGTC CCAGAAGAAG TAGCAGGGAA AAGGAACAGA GTAAAACTGG TGGCTCTTCA 
AAATTCGATT GGGCTGCTCG TTTCAGCCCT AAAGTTAGCC TTCCTAAAAC CAAACTGTCT 
CTTCCAGGGT CTTCTAAGTC AGAGACATCA AAACCTGGAC CTTCTGGATT ACAGGCCAAA 
TTAGCAAGTT TAAGAAAATC TACCAAGAAG CGCAGTGAAT CCCCACCTGC TGAGCTCCCC 
AGTTTGAGGC GGAGCACACG CCAAAAGACC ACGGGCTCCT GTGCTAGTGC CAGTCGGCGA 
GGCTCTGGCC TGGGCAAAAG AGGAGCAGCT GAAGCTCGTC GACAGGAGAA GATGGCGGAC 
CCTGAAAGCA GCCAGGAGAC CGTGAATTCT TCAGCTGCAC GGACAGACGA AACTCCCCAA 
GGAGCTGCAG CCTCTAGTTC TGTTGCAGGG GCTGTGGGCA TGACCACCTC TGGGGAGAGT 
GAATCCGATG ATTCTGAGAT GGGACGACTA CAAGCTTTGT TAGAGGCCAG GGGTCTTCCT 
CCTCATCTGT TTGGCCCTCT CGGTCCTCGG ATGTCACAAC TTTTCCATAG AACAATTGGA 
AGCGGAGCTA GCTCTAAGGC CCAGCAGCTT CTGCAAGGAC TACAAGCAAG TGATGAAAGC 
CAGCAACTCC AGGCAGTTAT TGAAATGTGT CAGTTATTGG TTATGGGAAA TGAGGAGACG 
CTGGGAGGGT TTCCGGTCAA GAGTGTCGTT CCAGCTTTGA TAACATTACT GCAGATGGAG 
CATAATTTCG ATATTATGAA CCATGCTTGT CGAGCCTTAA CATATATGAT GGAAGCACTT 
CCTCGGTCAT CTGCTGTTGT AGTAGATGCA ATTCCTGTCT TTTTAGAAAA GCTGCAAGTT 
ATTCAGTGTA TTGATGTCGC AGAGCAGGCC CTGACTGCCT TGGAGATGTT ATCACGCAGA 
CACAGTAAAG CCATTCTACA GGCGGGTGGT TTGGCAGACT GCTTGCTGTA TCTAGAATTC 
TTCAGCATAA ATGCCCAAAG AAATGCACTA GCAATTGCAG CCAATTGCTG CCAGAGTATC 
ACGCCAGATG AATTTCATTT TGTGGCAGAT TCCCTCCCGT TGCTTACCCA AAGGCTAACC 
CATCAGGACA AAAAGTCAGT TGAAAGCACT TGCCTTTGTT TTGCACGTCT AGTGGACAAC 
TTCCAACATG AGGAGAATTT ACTCCAGCAG GTTGCCTCCA AAGATCTGCT TACGAATGTT 
CAACAACTCT TGGTAGTGAC TCCACCCATT TTAAGTTCTG GGATGTTTAT AATGGTGGTT 
CGCATGTTTT CTCTGATGTG TTCCAACTGT CCAACTTTAG CTGTTCAACT TATGAAGCAA 
AACATTGCAG AAACACTTCA CTTTCTCCTG TGTGGTGCCT CTAATGGAAG TTGTCAGGAA 
CAGATTGATC TTGTTCCACG AAGTCCACAA GAACTGTATG AACTGACATC TCTGATTTGC 
GAACTTATGC CATGTTTACC AAAAGAAGGC ATTTTTGCAG TTGATACCAT GCTGAAGAAG 
GGAAATGCGC AGAACACAGA CGGTGCGATT TGGCAGTGGC GTGATGACCG TGGCCTTTGG 
CATCCCTATA ACAGGATTGA CAGCCGGATC ATTGAGGTAC AAATCAATGA GGACACGGGA 
ACAGCACGTG CCATTCAGAG AAAACCTAAC CCCTTAGCCA ATACTAACAC TAGTGGATAT 
TCAGAGTTAA AGAAGGATGA TGCTCGAGCA CAGCTTATGA AAGAGGATCC GGAACTGGCT 
AAGTCTTTTA TTAAGACATT ATTTGGTGTT CTTTATGAAG TGTATAGTTC CTCAGCAGGA 
CCTGCGGTCA GACATAAGTG CCTTAGAGCA ATTCTTAGGA TAATTTATTT TGCGGATGCT 
GAACTTCTGA AGGATGTCCT GAAAAATCAT GCTGTTTCAA GTCACATTGC TTCCATGCTG 
TCAAGCCAAG ACCTGAAGAT AGTAGTTGGA GCACTTCAGA TGGCAGAAAT CTTAATGCAG 
AAGTTACCTG ATATTTTTAG TGTTTACTTC AGAAGAGAAG GTGTAATGCA TCAAGTAAAA 
CACTTAGCAG AATCAGAGTC TTTGTTGACA AGTCCACCAA AGGCATGTAC GAATGGGTCA 
GGATCCCTGG GATCCACGAC GTCAGTCAGC AGTGGAACAG CCACGGCCGC CACCAATGCT 
GCAGCTGACC TGGGGTCCCC CAGCTTGCAG CACAGCAGAG ATGACTCTTT AGATCTGAGC 
CCTCAAGGTC GATTAAGTGA TGTTCTAAAG AGAAAACGGC TGCCAAAACG AGGGCCAAGA 
AGGCCAAAGT ATTCACCTCC AAGAGATGAT GACAAAGTAG ACAATCAAGC TAAAAGCCCT 
ACCACTACTC AGTCACCTAA ATCTTCTTTC CTGGCAAGCT TGAATCCAAA AACGTGGGGA 
AGGTTAAGCG CACAGTCCAA CAGCAACAAC ATCGAACCAG CACGAACTGC GGGAGTCAGT 
GGTCTTGCCA GGGCCGCCTC CAAGGACACC ATATCCAATA ACAGAGAAAA AATTAAAGGT 
TGGATTAAGG AACAGGCGCA TAAATTTGTA GAGCGTTATT TCAGTTCTGA GAACATGGAT 
GGAAGCAACC CTGCACTGAA TGTCCTTCAG AGACTTTGTG CTGCAACTGA ACAACTCAAC 
CTCCAGGTGG ATGGTGGAGC TGAGTGCCTT GTAGAAATCC GTAGCATAGT CTCAGAGTCT 
GATGTTTCAT CATTTGAAAT CCAACATAGT GGATTTGTGA AGCAGCTGTT ACTTTATTTG 
ACATCTAAAA GTGAAAAGGA TGCTGTGAGC CGAGAGATCA GATTAAAGAG ATTCCTTCAC 
GTATTTTTTT CTTCACCACT TCCTGGAGAA GAGCCCATTG GAAGAGTAGA ACCAGTGGGT 
AATGCGCCTT TGTTGGCATT AGTTCACAAG ATGAACAACT GCCTCAGCCA GATGGAACAG 
TTTCCAGTCA AAGTACATGA CTTCCCCAGT GGGAACGGGA CAGGAGGCAG CTTTTCTCTC 
AACAGAGGAT CACAGGCTTT AAAATTTTTC AACACACACC AGTTAAAATG CCAGTTACAA 
AGACATCCAG ACTGTGCAAA TGTGAAGCAG TGGAAGGGCG GGCCGGTCAA GATTGATCCC 
CTGGCTTTGG TACAAGCTAT TGAGAGATAC CTTGTAGTTA GAGGGTATGG AAGAGTAAGA 
GAAGATGATG AAGACAGTGA TGATGATGGC TCTGATGAGG AAATAGACGA GTCTCTGGCC 
GCTCAGTTCT TAAATTCCGG AAACGTAAGG CACAGGCTAC AGTTTTATAT TGGAGAACAT 
TTGCTACCAT ATAACATGAC TGTATATCAG GCAGTCCGGC AGTTCAGTAT ACAAGCTGAG 
GATGAGCGGG AGTCCACAGA TGATGAGAGC AACCCTCTGG GAAGAGCTGG TATTTGGACA 
AAGACTCATA CAATATGGTA CAAACCTGTG AGAGAGGATG AAGAAAGTAA TAAAGATTGT 
GTTGGTGGTA AAAGAGGAAG AGCCCAAACG GCTCCAACAA AAACATCCCC TAGAAATGCA 
AAAAAGCATG ATGAGTTATG GCATGATGGA GTATGCCCAT CAGTATCCAA TCCTTTAGAA 
GTTTACCTCA TCTCCTCGCC ACCTGAGAAT ATAACTTTTG AAGACCCGTC CTTAGATGTG 
ATTCTTCTTC TAAGAGTTTT ACATGCCATC AGCCGATACT GGTATTACTT GTATGATAAT 
GCAATGTGCA AGGAGATTAT TCCAACTAGT GAATTTATTA ACAGTAAGTT GACAGCAAAA 
GCAAATAGGC AGCTTCAAGA TCCTTTAGTA ATCATGACAG GAAATATCCC AACGTGGCTT 
ACTGAACTAG GAAAAACCTG CCCATTTTTC TTCCCTTTTG ATACCCGGCA AATGCTTTTT 
TACGTAACTG CATTTGATCG GGACCGAGCA ATGCAAAGAT TACTTGATAC CAACCCAGAA 
ATCAACCAGT CGGATTCTCA AGATAGCAGA GTTGCACCTA GATTGGATAG AAAAAAACGT 
ACTGTGAACA GAGAGGAGCT GCTGAAACAA GCGGAGTCTG TAATGCAGGA TCTCGGTAGC 
TCACGGGCCA TGTTAGAAAT CCAGTATGAA AATGAGGTTG GTACAGGTCT TGGGCCTACA 
CTGGAGTTTT ATGCACTTGT ATCTCAGGAA CTTCAGAGAG CTGACTTGGG TCTCTGGAGA 
GGTGAAGAAG TGACTCTCAG CAATCCAAAA GGAAGCCAAG AAGGGACCAA GTATATTCAA 
AACCTCCAGG GCCTGTTTGC ACTTCCCTTT GGTAGGACAG CTAAGCCAGC CCATATCGCA 
AAGGTTAAAA TGAAGTTTCG CTTCTTAGGA AAATTAATGG CCAAGGCTAT CATGGATTTC 
AGATTGGTGG ACCTTCCCCT TGGTTTACCC TTTTATAAGT GGATGCTAAG GCAAGAAACT 
TCACTGACGT CGCATGATTT GTTTGACATT GACCCAGTTG TAGCCAGATC AGTGTATCAC 
CTAGAAGACA TTGTCAGACA GAAGAAAAGA CTTGAGCAAG ATAAATCCCA GACCAAAGAG 
AGTCTACAAT ATGCATTAGA AACCCTGACT ATGAATGGCT GCTCAGTTGA AGATCTAGGA 
TTGGATTTCA CGCTGCCAGG GTTTCCCAAC ATTGAACTGA AGAAAGGAGG AAAGGATATA 
CCAGTTACCA TCCACAATTT AGAGGAGTAT CTAAGACTGG TTATATTCTG GGCACTAAAT 
GAAGGCGTTT CCAGGCAATT TGATTCCTTC AGAGATGGAT TTGAATCTGT CTTCCCACTT 
AGTCATCTTC AGTACTTCTA CCCGGAAGAA CTGGATCAGC TCTTGTGCGG CAGTAAAGCA 
GACACGTGGG ATGCGAAGAC ACTGATGGAG TGCTGCAGGC CGGACCACGG TTATACTCAT 
GACAGTCGGG CTGTGAAGTT CTTGTTTGAG ATTCTCAGTA GTTTTGATAA TGAGCAGCAG 
AGGTTGTTTC TCCAGTTTGT GACAGGTAGC CCAAGATTGC CTGTTGGAGG CTTCCGGAGT 
CTGAATCCAC CTCTGACAAT TGTCCGGAAG ACCTTCGAGT CCACGGAGAA CCCCGACGAC 
TTCTTGCCCT CTGTAATGAC TTGTGTGAAC TACCTTAAGT TGCCGGACTA CTCGAGCATT 
GACATCATGC GTGAGAAACT GTTGATTGCA GCGAGAGAAG GGCAGCAGTC ATTCCATCTT 
TCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005635861.1
CDS	188..6313
Translation	MSNRPNNNPGGSLRRSQRNTAGAQPQDDSIGGRSHLGQAKHKGYSPPESRKSNSKAPKVQSNTTSELSRGHLSKRSCSSSSAVIVPQPEDPDRANTSERQKTGQVPKKDNSRGVKRSASPDYNRTNSPSSAKKPKALQHTESPSETNKPHSKSKKRHLDQEQQLKSAQSPSTSKAHTRKSGATGGSRSQKRKRTESSCLKSGSVSESTGAEERSAKPTKLASKSAASAKAGCSTITDSSSAASTSSSSSAVASASSTVPPGARVKQGKDQSKARRSRSASSPSPRRSSREKEQSKTGGSSKFDWAARFSPKVSLPKTKLSLPGSSKSETSKPGPSGLQAKLASLRKSTKKRSESPPAELPSLRRSTRQKTTGSCASASRRGSGLGKRGAAEARRQEKMADPESSQETVNSSAARTDETPQGAAASSSVAGAVGMTTSGESESDDSEMGRLQALLEARGLPPHLFGPLGPRMSQLFHRTIGSGASSKAQQLLQGLQASDESQQLQAVIEMCQLLVMGNEETLGGFPVKSVVPALITLLQMEHNFDIMNHACRALTYMMEALPRSSAVVVDAIPVFLEKLQVIQCIDVAEQALTALEMLSRRHSKAILQAGGLADCLLYLEFFSINAQRNALAIAANCCQSITPDEFHFVADSLPLLTQRLTHQDKKSVESTCLCFARLVDNFQHEENLLQQVASKDLLTNVQQLLVVTPPILSSGMFIMVVRMFSLMCSNCPTLAVQLMKQNIAETLHFLLCGASNGSCQEQIDLVPRSPQELYELTSLICELMPCLPKEGIFAVDTMLKKGNAQNTDGAIWQWRDDRGLWHPYNRIDSRIIEVQINEDTGTARAIQRKPNPLANTNTSGYSELKKDDARAQLMKEDPELAKSFIKTLFGVLYEVYSSSAGPAVRHKCLRAILRIIYFADAELLKDVLKNHAVSSHIASMLSSQDLKIVVGALQMAEILMQKLPDIFSVYFRREGVMHQVKHLAESESLLTSPPKACTNGSGSLGSTTSVSSGTATAATNAAADLGSPSLQHSRDDSLDLSPQGRLSDVLKRKRLPKRGPRRPKYSPPRDDDKVDNQAKSPTTTQSPKSSFLASLNPKTWGRLSAQSNSNNIEPARTAGVSGLARAASKDTISNNREKIKGWIKEQAHKFVERYFSSENMDGSNPALNVLQRLCAATEQLNLQVDGGAECLVEIRSIVSESDVSSFEIQHSGFVKQLLLYLTSKSEKDAVSREIRLKRFLHVFFSSPLPGEEPIGRVEPVGNAPLLALVHKMNNCLSQMEQFPVKVHDFPSGNGTGGSFSLNRGSQALKFFNTHQLKCQLQRHPDCANVKQWKGGPVKIDPLALVQAIERYLVVRGYGRVREDDEDSDDDGSDEEIDESLAAQFLNSGNVRHRLQFYIGEHLLPYNMTVYQAVRQFSIQAEDERESTDDESNPLGRAGIWTKTHTIWYKPVREDEESNKDCVGGKRGRAQTAPTKTSPRNAKKHDELWHDGVCPSVSNPLEVYLISSPPENITFEDPSLDVILLLRVLHAISRYWYYLYDNAMCKEIIPTSEFINSKLTAKANRQLQDPLVIMTGNIPTWLTELGKTCPFFFPFDTRQMLFYVTAFDRDRAMQRLLDTNPEINQSDSQDSRVAPRLDRKKRTVNREELLKQAESVMQDLGSSRAMLEIQYENEVGTGLGPTLEFYALVSQELQRADLGLWRGEEVTLSNPKGSQEGTKYIQNLQGLFALPFGRTAKPAHIAKVKMKFRFLGKLMAKAIMDFRLVDLPLGLPFYKWMLRQETSLTSHDLFDIDPVVARSVYHLEDIVRQKKRLEQDKSQTKESLQYALETLTMNGCSVEDLGLDFTLPGFPNIELKKGGKDIPVTIHNLEEYLRLVIFWALNEGVSRQFDSFRDGFESVFPLSHLQYFYPEELDQLLCGSKADTWDAKTLMECCRPDHGYTHDSRAVKFLFEILSSFDNEQQRLFLQFVTGSPRLPVGGFRSLNPPLTIVRKTFESTENPDDFLPSVMTCVNYLKLPDYSSIDIMREKLLIAAREGQQSFHLS

Target ORF information:

RefSeq Version	XM_005635804.2
Organism	Canis lupus familiaris(Dog)
Definition	Canis lupus familiaris thyroid hormone receptor interactor 12 (TRIP12), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005635804.2

ORF Insert Sequence:

ATGTCCAACC GGCCTAATAA CAATCCAGGG GGGTCACTGC GACGTTCACA GAGGAACACT 
GCCGGGGCCC AACCACAAGA CGACTCGATA GGAGGAAGGT CACATTTAGG GCAGGCAAAA 
CATAAGGGAT ATAGCCCTCC TGAGAGTAGA AAATCTAATT CTAAGGCACC CAAAGTGCAG 
TCTAATACTA CTTCTGAACT GTCAAGGGGA CACCTTTCTA AAAGAAGCTG CAGTTCATCA 
TCTGCTGTCA TAGTTCCACA ACCAGAGGAT CCAGACAGAG CCAATACTTC AGAAAGGCAA 
AAAACGGGGC AGGTGCCTAA GAAAGACAAT TCTCGAGGAG TGAAACGCAG TGCTAGTCCA 
GACTACAACA GGACCAATTC TCCTAGCTCT GCCAAAAAAC CAAAAGCACT TCAGCATACT 
GAATCTCCCT CAGAAACAAA TAAGCCACAT AGTAAGTCGA AGAAGAGACA TTTAGACCAG 
GAGCAACAAC TGAAATCTGC ACAGTCGCCA TCAACAAGTA AGGCTCACAC CAGAAAGAGT 
GGGGCCACCG GCGGCTCACG GAGTCAGAAA AGAAAGAGGA CAGAGAGTTC TTGTCTAAAG 
AGTGGTTCTG TGTCTGAGTC AACTGGTGCC GAAGAGAGAT CTGCAAAACC CACCAAGCTG 
GCTTCAAAAT CCGCCGCCTC GGCCAAAGCT GGGTGCAGCA CCATCACTGA TTCTTCCTCC 
GCTGCCTCTA CTTCCTCCTC ATCTTCTGCT GTCGCCTCGG CCTCCTCCAC TGTACCACCA 
GGGGCCAGGG TGAAACAAGG AAAAGACCAG AGCAAGGCCA GGCGTTCCCG TTCAGCATCC 
AGTCCCAGTC CCAGAAGAAG TAGCAGGGAA AAGGAACAGA GTAAAACTGG TGGCTCTTCA 
AAATTCGATT GGGCTGCTCG TTTCAGCCCT AAAGTTAGCC TTCCTAAAAC CAAACTGTCT 
CTTCCAGGGT CTTCTAAGTC AGAGACATCA AAACCTGGAC CTTCTGGATT ACAGGCCAAA 
TTAGCAAGTT TAAGAAAATC TACCAAGAAG CGCAGTGAAT CCCCACCTGC TGAGCTCCCC 
AGTTTGAGGC GGAGCACACG CCAAAAGACC ACGGGCTCCT GTGCTAGTGC CAGTCGGCGA 
GGCTCTGGCC TGGGCAAAAG AGGAGCAGCT GAAGCTCGTC GACAGGAGAA GATGGCGGAC 
CCTGAAAGCA GCCAGGAGAC CGTGAATTCT TCAGCTGCAC GGACAGACGA AACTCCCCAA 
GGAGCTGCAG CCTCTAGTTC TGTTGCAGGG GCTGTGGGCA TGACCACCTC TGGGGAGAGT 
GAATCCGATG ATTCTGAGAT GGGACGACTA CAAGCTTTGT TAGAGGCCAG GGGTCTTCCT 
CCTCATCTGT TTGGCCCTCT CGGTCCTCGG ATGTCACAAC TTTTCCATAG AACAATTGGA 
AGCGGAGCTA GCTCTAAGGC CCAGCAGCTT CTGCAAGGAC TACAAGCAAG TGATGAAAGC 
CAGCAACTCC AGGCAGTTAT TGAAATGTGT CAGTTATTGG TTATGGGAAA TGAGGAGACG 
CTGGGAGGGT TTCCGGTCAA GAGTGTCGTT CCAGCTTTGA TAACATTACT GCAGATGGAG 
CATAATTTCG ATATTATGAA CCATGCTTGT CGAGCCTTAA CATATATGAT GGAAGCACTT 
CCTCGGTCAT CTGCTGTTGT AGTAGATGCA ATTCCTGTCT TTTTAGAAAA GCTGCAAGTT 
ATTCAGTGTA TTGATGTCGC AGAGCAGGCC CTGACTGCCT TGGAGATGTT ATCACGCAGA 
CACAGTAAAG CCATTCTACA GGCGGGTGGT TTGGCAGACT GCTTGCTGTA TCTAGAATTC 
TTCAGCATAA ATGCCCAAAG AAATGCACTA GCAATTGCAG CCAATTGCTG CCAGAGTATC 
ACGCCAGATG AATTTCATTT TGTGGCAGAT TCCCTCCCGT TGCTTACCCA AAGGCTAACC 
CATCAGGACA AAAAGTCAGT TGAAAGCACT TGCCTTTGTT TTGCACGTCT AGTGGACAAC 
TTCCAACATG AGGAGAATTT ACTCCAGCAG GTTGCCTCCA AAGATCTGCT TACGAATGTT 
CAACAACTCT TGGTAGTGAC TCCACCCATT TTAAGTTCTG GGATGTTTAT AATGGTGGTT 
CGCATGTTTT CTCTGATGTG TTCCAACTGT CCAACTTTAG CTGTTCAACT TATGAAGCAA 
AACATTGCAG AAACACTTCA CTTTCTCCTG TGTGGTGCCT CTAATGGAAG TTGTCAGGAA 
CAGATTGATC TTGTTCCACG AAGTCCACAA GAACTGTATG AACTGACATC TCTGATTTGC 
GAACTTATGC CATGTTTACC AAAAGAAGGC ATTTTTGCAG TTGATACCAT GCTGAAGAAG 
GGAAATGCGC AGAACACAGA CGGTGCGATT TGGCAGTGGC GTGATGACCG TGGCCTTTGG 
CATCCCTATA ACAGGATTGA CAGCCGGATC ATTGAGGTAC AAATCAATGA GGACACGGGA 
ACAGCACGTG CCATTCAGAG AAAACCTAAC CCCTTAGCCA ATACTAACAC TAGTGGATAT 
TCAGAGTTAA AGAAGGATGA TGCTCGAGCA CAGCTTATGA AAGAGGATCC GGAACTGGCT 
AAGTCTTTTA TTAAGACATT ATTTGGTGTT CTTTATGAAG TGTATAGTTC CTCAGCAGGA 
CCTGCGGTCA GACATAAGTG CCTTAGAGCA ATTCTTAGGA TAATTTATTT TGCGGATGCT 
GAACTTCTGA AGGATGTCCT GAAAAATCAT GCTGTTTCAA GTCACATTGC TTCCATGCTG 
TCAAGCCAAG ACCTGAAGAT AGTAGTTGGA GCACTTCAGA TGGCAGAAAT CTTAATGCAG 
AAGTTACCTG ATATTTTTAG TGTTTACTTC AGAAGAGAAG GTGTAATGCA TCAAGTAAAA 
CACTTAGCAG AATCAGAGTC TTTGTTGACA AGTCCACCAA AGGCATGTAC GAATGGGTCA 
GGATCCCTGG GATCCACGAC GTCAGTCAGC AGTGGAACAG CCACGGCCGC CACCAATGCT 
GCAGCTGACC TGGGGTCCCC CAGCTTGCAG CACAGCAGAG ATGACTCTTT AGATCTGAGC 
CCTCAAGGTC GATTAAGTGA TGTTCTAAAG AGAAAACGGC TGCCAAAACG AGGGCCAAGA 
AGGCCAAAGT ATTCACCTCC AAGAGATGAT GACAAAGTAG ACAATCAAGC TAAAAGCCCT 
ACCACTACTC AGTCACCTAA ATCTTCTTTC CTGGCAAGCT TGAATCCAAA AACGTGGGGA 
AGGTTAAGCG CACAGTCCAA CAGCAACAAC ATCGAACCAG CACGAACTGC GGGAGTCAGT 
GGTCTTGCCA GGGCCGCCTC CAAGGACACC ATATCCAATA ACAGAGAAAA AATTAAAGGT 
TGGATTAAGG AACAGGCGCA TAAATTTGTA GAGCGTTATT TCAGTTCTGA GAACATGGAT 
GGAAGCAACC CTGCACTGAA TGTCCTTCAG AGACTTTGTG CTGCAACTGA ACAACTCAAC 
CTCCAGGTGG ATGGTGGAGC TGAGTGCCTT GTAGAAATCC GTAGCATAGT CTCAGAGTCT 
GATGTTTCAT CATTTGAAAT CCAACATAGT GGATTTGTGA AGCAGCTGTT ACTTTATTTG 
ACATCTAAAA GTGAAAAGGA TGCTGTGAGC CGAGAGATCA GATTAAAGAG ATTCCTTCAC 
GTATTTTTTT CTTCACCACT TCCTGGAGAA GAGCCCATTG GAAGAGTAGA ACCAGTGGGT 
AATGCGCCTT TGTTGGCATT AGTTCACAAG ATGAACAACT GCCTCAGCCA GATGGAACAG 
TTTCCAGTCA AAGTACATGA CTTCCCCAGT GGGAACGGGA CAGGAGGCAG CTTTTCTCTC 
AACAGAGGAT CACAGGCTTT AAAATTTTTC AACACACACC AGTTAAAATG CCAGTTACAA 
AGACATCCAG ACTGTGCAAA TGTGAAGCAG TGGAAGGGCG GGCCGGTCAA GATTGATCCC 
CTGGCTTTGG TACAAGCTAT TGAGAGATAC CTTGTAGTTA GAGGGTATGG AAGAGTAAGA 
GAAGATGATG AAGACAGTGA TGATGATGGC TCTGATGAGG AAATAGACGA GTCTCTGGCC 
GCTCAGTTCT TAAATTCCGG AAACGTAAGG CACAGGCTAC AGTTTTATAT TGGAGAACAT 
TTGCTACCAT ATAACATGAC TGTATATCAG GCAGTCCGGC AGTTCAGTAT ACAAGCTGAG 
GATGAGCGGG AGTCCACAGA TGATGAGAGC AACCCTCTGG GAAGAGCTGG TATTTGGACA 
AAGACTCATA CAATATGGTA CAAACCTGTG AGAGAGGATG AAGAAAGTAA TAAAGATTGT 
GTTGGTGGTA AAAGAGGAAG AGCCCAAACG GCTCCAACAA AAACATCCCC TAGAAATGCA 
AAAAAGCATG ATGAGTTATG GCATGATGGA GTATGCCCAT CAGTATCCAA TCCTTTAGAA 
GTTTACCTCA TCTCCTCGCC ACCTGAGAAT ATAACTTTTG AAGACCCGTC CTTAGATGTG 
ATTCTTCTTC TAAGAGTTTT ACATGCCATC AGCCGATACT GGTATTACTT GTATGATAAT 
GCAATGTGCA AGGAGATTAT TCCAACTAGT GAATTTATTA ACAGTAAGTT GACAGCAAAA 
GCAAATAGGC AGCTTCAAGA TCCTTTAGTA ATCATGACAG GAAATATCCC AACGTGGCTT 
ACTGAACTAG GAAAAACCTG CCCATTTTTC TTCCCTTTTG ATACCCGGCA AATGCTTTTT 
TACGTAACTG CATTTGATCG GGACCGAGCA ATGCAAAGAT TACTTGATAC CAACCCAGAA 
ATCAACCAGT CGGATTCTCA AGATAGCAGA GTTGCACCTA GATTGGATAG AAAAAAACGT 
ACTGTGAACA GAGAGGAGCT GCTGAAACAA GCGGAGTCTG TAATGCAGGA TCTCGGTAGC 
TCACGGGCCA TGTTAGAAAT CCAGTATGAA AATGAGGTTG GTACAGGTCT TGGGCCTACA 
CTGGAGTTTT ATGCACTTGT ATCTCAGGAA CTTCAGAGAG CTGACTTGGG TCTCTGGAGA 
GGTGAAGAAG TGACTCTCAG CAATCCAAAA GGAAGCCAAG AAGGGACCAA GTATATTCAA 
AACCTCCAGG GCCTGTTTGC ACTTCCCTTT GGTAGGACAG CTAAGCCAGC CCATATCGCA 
AAGGTTAAAA TGAAGTTTCG CTTCTTAGGA AAATTAATGG CCAAGGCTAT CATGGATTTC 
AGATTGGTGG ACCTTCCCCT TGGTTTACCC TTTTATAAGT GGATGCTAAG GCAAGAAACT 
TCACTGACGT CGCATGATTT GTTTGACATT GACCCAGTTG TAGCCAGATC AGTGTATCAC 
CTAGAAGACA TTGTCAGACA GAAGAAAAGA CTTGAGCAAG ATAAATCCCA GACCAAAGAG 
AGTCTACAAT ATGCATTAGA AACCCTGACT ATGAATGGCT GCTCAGTTGA AGATCTAGGA 
TTGGATTTCA CGCTGCCAGG GTTTCCCAAC ATTGAACTGA AGAAAGGAGG AAAGGATATA 
CCAGTTACCA TCCACAATTT AGAGGAGTAT CTAAGACTGG TTATATTCTG GGCACTAAAT 
GAAGGCGTTT CCAGGCAATT TGATTCCTTC AGAGATGGAT TTGAATCTGT CTTCCCACTT 
AGTCATCTTC AGTACTTCTA CCCGGAAGAA CTGGATCAGC TCTTGTGCGG CAGTAAAGCA 
GACACGTGGG ATGCGAAGAC ACTGATGGAG TGCTGCAGGC CGGACCACGG TTATACTCAT 
GACAGTCGGG CTGTGAAGTT CTTGTTTGAG ATTCTCAGTA GTTTTGATAA TGAGCAGCAG 
AGGTTGTTTC TCCAGTTTGT GACAGGTAGC CCAAGATTGC CTGTTGGAGG CTTCCGGAGT 
CTGAATCCAC CTCTGACAAT TGTCCGGAAG ACCTTCGAGT CCACGGAGAA CCCCGACGAC 
TTCTTGCCCT CTGTAATGAC TTGTGTGAAC TACCTTAAGT TGCCGGACTA CTCGAGCATT 
GACATCATGC GTGAGAAACT GTTGATTGCA GCGAGAGAAG GGCAGCAGTC ATTCCATCTT 
TCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TRIP12 ( XM_022409667.1 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_022265375.1 Canis lupus familiaris thyroid hormone receptor interactor 12 (TRIP12), transcript variant X7, mRNA.

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CloneID	OCe25764
Clone ID Related Accession (Same CDS sequence)	XM_005635804.2 , XM_022409667.1
Accession Version	XM_022409667.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6126bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-09-04
Organism	Canis lupus familiaris(dog)
Product	E3 ubiquitin-protein ligase TRIP12 isoform X5
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006607.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCCAACC GGCCTAATAA CAATCCAGGG GGGTCACTGC GACGTTCACA GAGGAACACT 
GCCGGGGCCC AACCACAAGA CGACTCGATA GGAGGAAGGT CACATTTAGG GCAGGCAAAA 
CATAAGGGAT ATAGCCCTCC TGAGAGTAGA AAATCTAATT CTAAGGCACC CAAAGTGCAG 
TCTAATACTA CTTCTGAACT GTCAAGGGGA CACCTTTCTA AAAGAAGCTG CAGTTCATCA 
TCTGCTGTCA TAGTTCCACA ACCAGAGGAT CCAGACAGAG CCAATACTTC AGAAAGGCAA 
AAAACGGGGC AGGTGCCTAA GAAAGACAAT TCTCGAGGAG TGAAACGCAG TGCTAGTCCA 
GACTACAACA GGACCAATTC TCCTAGCTCT GCCAAAAAAC CAAAAGCACT TCAGCATACT 
GAATCTCCCT CAGAAACAAA TAAGCCACAT AGTAAGTCGA AGAAGAGACA TTTAGACCAG 
GAGCAACAAC TGAAATCTGC ACAGTCGCCA TCAACAAGTA AGGCTCACAC CAGAAAGAGT 
GGGGCCACCG GCGGCTCACG GAGTCAGAAA AGAAAGAGGA CAGAGAGTTC TTGTCTAAAG 
AGTGGTTCTG TGTCTGAGTC AACTGGTGCC GAAGAGAGAT CTGCAAAACC CACCAAGCTG 
GCTTCAAAAT CCGCCGCCTC GGCCAAAGCT GGGTGCAGCA CCATCACTGA TTCTTCCTCC 
GCTGCCTCTA CTTCCTCCTC ATCTTCTGCT GTCGCCTCGG CCTCCTCCAC TGTACCACCA 
GGGGCCAGGG TGAAACAAGG AAAAGACCAG AGCAAGGCCA GGCGTTCCCG TTCAGCATCC 
AGTCCCAGTC CCAGAAGAAG TAGCAGGGAA AAGGAACAGA GTAAAACTGG TGGCTCTTCA 
AAATTCGATT GGGCTGCTCG TTTCAGCCCT AAAGTTAGCC TTCCTAAAAC CAAACTGTCT 
CTTCCAGGGT CTTCTAAGTC AGAGACATCA AAACCTGGAC CTTCTGGATT ACAGGCCAAA 
TTAGCAAGTT TAAGAAAATC TACCAAGAAG CGCAGTGAAT CCCCACCTGC TGAGCTCCCC 
AGTTTGAGGC GGAGCACACG CCAAAAGACC ACGGGCTCCT GTGCTAGTGC CAGTCGGCGA 
GGCTCTGGCC TGGGCAAAAG AGGAGCAGCT GAAGCTCGTC GACAGGAGAA GATGGCGGAC 
CCTGAAAGCA GCCAGGAGAC CGTGAATTCT TCAGCTGCAC GGACAGACGA AACTCCCCAA 
GGAGCTGCAG CCTCTAGTTC TGTTGCAGGG GCTGTGGGCA TGACCACCTC TGGGGAGAGT 
GAATCCGATG ATTCTGAGAT GGGACGACTA CAAGCTTTGT TAGAGGCCAG GGGTCTTCCT 
CCTCATCTGT TTGGCCCTCT CGGTCCTCGG ATGTCACAAC TTTTCCATAG AACAATTGGA 
AGCGGAGCTA GCTCTAAGGC CCAGCAGCTT CTGCAAGGAC TACAAGCAAG TGATGAAAGC 
CAGCAACTCC AGGCAGTTAT TGAAATGTGT CAGTTATTGG TTATGGGAAA TGAGGAGACG 
CTGGGAGGGT TTCCGGTCAA GAGTGTCGTT CCAGCTTTGA TAACATTACT GCAGATGGAG 
CATAATTTCG ATATTATGAA CCATGCTTGT CGAGCCTTAA CATATATGAT GGAAGCACTT 
CCTCGGTCAT CTGCTGTTGT AGTAGATGCA ATTCCTGTCT TTTTAGAAAA GCTGCAAGTT 
ATTCAGTGTA TTGATGTCGC AGAGCAGGCC CTGACTGCCT TGGAGATGTT ATCACGCAGA 
CACAGTAAAG CCATTCTACA GGCGGGTGGT TTGGCAGACT GCTTGCTGTA TCTAGAATTC 
TTCAGCATAA ATGCCCAAAG AAATGCACTA GCAATTGCAG CCAATTGCTG CCAGAGTATC 
ACGCCAGATG AATTTCATTT TGTGGCAGAT TCCCTCCCGT TGCTTACCCA AAGGCTAACC 
CATCAGGACA AAAAGTCAGT TGAAAGCACT TGCCTTTGTT TTGCACGTCT AGTGGACAAC 
TTCCAACATG AGGAGAATTT ACTCCAGCAG GTTGCCTCCA AAGATCTGCT TACGAATGTT 
CAACAACTCT TGGTAGTGAC TCCACCCATT TTAAGTTCTG GGATGTTTAT AATGGTGGTT 
CGCATGTTTT CTCTGATGTG TTCCAACTGT CCAACTTTAG CTGTTCAACT TATGAAGCAA 
AACATTGCAG AAACACTTCA CTTTCTCCTG TGTGGTGCCT CTAATGGAAG TTGTCAGGAA 
CAGATTGATC TTGTTCCACG AAGTCCACAA GAACTGTATG AACTGACATC TCTGATTTGC 
GAACTTATGC CATGTTTACC AAAAGAAGGC ATTTTTGCAG TTGATACCAT GCTGAAGAAG 
GGAAATGCGC AGAACACAGA CGGTGCGATT TGGCAGTGGC GTGATGACCG TGGCCTTTGG 
CATCCCTATA ACAGGATTGA CAGCCGGATC ATTGAGGTAC AAATCAATGA GGACACGGGA 
ACAGCACGTG CCATTCAGAG AAAACCTAAC CCCTTAGCCA ATACTAACAC TAGTGGATAT 
TCAGAGTTAA AGAAGGATGA TGCTCGAGCA CAGCTTATGA AAGAGGATCC GGAACTGGCT 
AAGTCTTTTA TTAAGACATT ATTTGGTGTT CTTTATGAAG TGTATAGTTC CTCAGCAGGA 
CCTGCGGTCA GACATAAGTG CCTTAGAGCA ATTCTTAGGA TAATTTATTT TGCGGATGCT 
GAACTTCTGA AGGATGTCCT GAAAAATCAT GCTGTTTCAA GTCACATTGC TTCCATGCTG 
TCAAGCCAAG ACCTGAAGAT AGTAGTTGGA GCACTTCAGA TGGCAGAAAT CTTAATGCAG 
AAGTTACCTG ATATTTTTAG TGTTTACTTC AGAAGAGAAG GTGTAATGCA TCAAGTAAAA 
CACTTAGCAG AATCAGAGTC TTTGTTGACA AGTCCACCAA AGGCATGTAC GAATGGGTCA 
GGATCCCTGG GATCCACGAC GTCAGTCAGC AGTGGAACAG CCACGGCCGC CACCAATGCT 
GCAGCTGACC TGGGGTCCCC CAGCTTGCAG CACAGCAGAG ATGACTCTTT AGATCTGAGC 
CCTCAAGGTC GATTAAGTGA TGTTCTAAAG AGAAAACGGC TGCCAAAACG AGGGCCAAGA 
AGGCCAAAGT ATTCACCTCC AAGAGATGAT GACAAAGTAG ACAATCAAGC TAAAAGCCCT 
ACCACTACTC AGTCACCTAA ATCTTCTTTC CTGGCAAGCT TGAATCCAAA AACGTGGGGA 
AGGTTAAGCG CACAGTCCAA CAGCAACAAC ATCGAACCAG CACGAACTGC GGGAGTCAGT 
GGTCTTGCCA GGGCCGCCTC CAAGGACACC ATATCCAATA ACAGAGAAAA AATTAAAGGT 
TGGATTAAGG AACAGGCGCA TAAATTTGTA GAGCGTTATT TCAGTTCTGA GAACATGGAT 
GGAAGCAACC CTGCACTGAA TGTCCTTCAG AGACTTTGTG CTGCAACTGA ACAACTCAAC 
CTCCAGGTGG ATGGTGGAGC TGAGTGCCTT GTAGAAATCC GTAGCATAGT CTCAGAGTCT 
GATGTTTCAT CATTTGAAAT CCAACATAGT GGATTTGTGA AGCAGCTGTT ACTTTATTTG 
ACATCTAAAA GTGAAAAGGA TGCTGTGAGC CGAGAGATCA GATTAAAGAG ATTCCTTCAC 
GTATTTTTTT CTTCACCACT TCCTGGAGAA GAGCCCATTG GAAGAGTAGA ACCAGTGGGT 
AATGCGCCTT TGTTGGCATT AGTTCACAAG ATGAACAACT GCCTCAGCCA GATGGAACAG 
TTTCCAGTCA AAGTACATGA CTTCCCCAGT GGGAACGGGA CAGGAGGCAG CTTTTCTCTC 
AACAGAGGAT CACAGGCTTT AAAATTTTTC AACACACACC AGTTAAAATG CCAGTTACAA 
AGACATCCAG ACTGTGCAAA TGTGAAGCAG TGGAAGGGCG GGCCGGTCAA GATTGATCCC 
CTGGCTTTGG TACAAGCTAT TGAGAGATAC CTTGTAGTTA GAGGGTATGG AAGAGTAAGA 
GAAGATGATG AAGACAGTGA TGATGATGGC TCTGATGAGG AAATAGACGA GTCTCTGGCC 
GCTCAGTTCT TAAATTCCGG AAACGTAAGG CACAGGCTAC AGTTTTATAT TGGAGAACAT 
TTGCTACCAT ATAACATGAC TGTATATCAG GCAGTCCGGC AGTTCAGTAT ACAAGCTGAG 
GATGAGCGGG AGTCCACAGA TGATGAGAGC AACCCTCTGG GAAGAGCTGG TATTTGGACA 
AAGACTCATA CAATATGGTA CAAACCTGTG AGAGAGGATG AAGAAAGTAA TAAAGATTGT 
GTTGGTGGTA AAAGAGGAAG AGCCCAAACG GCTCCAACAA AAACATCCCC TAGAAATGCA 
AAAAAGCATG ATGAGTTATG GCATGATGGA GTATGCCCAT CAGTATCCAA TCCTTTAGAA 
GTTTACCTCA TCTCCTCGCC ACCTGAGAAT ATAACTTTTG AAGACCCGTC CTTAGATGTG 
ATTCTTCTTC TAAGAGTTTT ACATGCCATC AGCCGATACT GGTATTACTT GTATGATAAT 
GCAATGTGCA AGGAGATTAT TCCAACTAGT GAATTTATTA ACAGTAAGTT GACAGCAAAA 
GCAAATAGGC AGCTTCAAGA TCCTTTAGTA ATCATGACAG GAAATATCCC AACGTGGCTT 
ACTGAACTAG GAAAAACCTG CCCATTTTTC TTCCCTTTTG ATACCCGGCA AATGCTTTTT 
TACGTAACTG CATTTGATCG GGACCGAGCA ATGCAAAGAT TACTTGATAC CAACCCAGAA 
ATCAACCAGT CGGATTCTCA AGATAGCAGA GTTGCACCTA GATTGGATAG AAAAAAACGT 
ACTGTGAACA GAGAGGAGCT GCTGAAACAA GCGGAGTCTG TAATGCAGGA TCTCGGTAGC 
TCACGGGCCA TGTTAGAAAT CCAGTATGAA AATGAGGTTG GTACAGGTCT TGGGCCTACA 
CTGGAGTTTT ATGCACTTGT ATCTCAGGAA CTTCAGAGAG CTGACTTGGG TCTCTGGAGA 
GGTGAAGAAG TGACTCTCAG CAATCCAAAA GGAAGCCAAG AAGGGACCAA GTATATTCAA 
AACCTCCAGG GCCTGTTTGC ACTTCCCTTT GGTAGGACAG CTAAGCCAGC CCATATCGCA 
AAGGTTAAAA TGAAGTTTCG CTTCTTAGGA AAATTAATGG CCAAGGCTAT CATGGATTTC 
AGATTGGTGG ACCTTCCCCT TGGTTTACCC TTTTATAAGT GGATGCTAAG GCAAGAAACT 
TCACTGACGT CGCATGATTT GTTTGACATT GACCCAGTTG TAGCCAGATC AGTGTATCAC 
CTAGAAGACA TTGTCAGACA GAAGAAAAGA CTTGAGCAAG ATAAATCCCA GACCAAAGAG 
AGTCTACAAT ATGCATTAGA AACCCTGACT ATGAATGGCT GCTCAGTTGA AGATCTAGGA 
TTGGATTTCA CGCTGCCAGG GTTTCCCAAC ATTGAACTGA AGAAAGGAGG AAAGGATATA 
CCAGTTACCA TCCACAATTT AGAGGAGTAT CTAAGACTGG TTATATTCTG GGCACTAAAT 
GAAGGCGTTT CCAGGCAATT TGATTCCTTC AGAGATGGAT TTGAATCTGT CTTCCCACTT 
AGTCATCTTC AGTACTTCTA CCCGGAAGAA CTGGATCAGC TCTTGTGCGG CAGTAAAGCA 
GACACGTGGG ATGCGAAGAC ACTGATGGAG TGCTGCAGGC CGGACCACGG TTATACTCAT 
GACAGTCGGG CTGTGAAGTT CTTGTTTGAG ATTCTCAGTA GTTTTGATAA TGAGCAGCAG 
AGGTTGTTTC TCCAGTTTGT GACAGGTAGC CCAAGATTGC CTGTTGGAGG CTTCCGGAGT 
CTGAATCCAC CTCTGACAAT TGTCCGGAAG ACCTTCGAGT CCACGGAGAA CCCCGACGAC 
TTCTTGCCCT CTGTAATGAC TTGTGTGAAC TACCTTAAGT TGCCGGACTA CTCGAGCATT 
GACATCATGC GTGAGAAACT GTTGATTGCA GCGAGAGAAG GGCAGCAGTC ATTCCATCTT 
TCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_022265375.1
CDS	1..6126
Translation	MSNRPNNNPGGSLRRSQRNTAGAQPQDDSIGGRSHLGQAKHKGYSPPESRKSNSKAPKVQSNTTSELSRGHLSKRSCSSSSAVIVPQPEDPDRANTSERQKTGQVPKKDNSRGVKRSASPDYNRTNSPSSAKKPKALQHTESPSETNKPHSKSKKRHLDQEQQLKSAQSPSTSKAHTRKSGATGGSRSQKRKRTESSCLKSGSVSESTGAEERSAKPTKLASKSAASAKAGCSTITDSSSAASTSSSSSAVASASSTVPPGARVKQGKDQSKARRSRSASSPSPRRSSREKEQSKTGGSSKFDWAARFSPKVSLPKTKLSLPGSSKSETSKPGPSGLQAKLASLRKSTKKRSESPPAELPSLRRSTRQKTTGSCASASRRGSGLGKRGAAEARRQEKMADPESSQETVNSSAARTDETPQGAAASSSVAGAVGMTTSGESESDDSEMGRLQALLEARGLPPHLFGPLGPRMSQLFHRTIGSGASSKAQQLLQGLQASDESQQLQAVIEMCQLLVMGNEETLGGFPVKSVVPALITLLQMEHNFDIMNHACRALTYMMEALPRSSAVVVDAIPVFLEKLQVIQCIDVAEQALTALEMLSRRHSKAILQAGGLADCLLYLEFFSINAQRNALAIAANCCQSITPDEFHFVADSLPLLTQRLTHQDKKSVESTCLCFARLVDNFQHEENLLQQVASKDLLTNVQQLLVVTPPILSSGMFIMVVRMFSLMCSNCPTLAVQLMKQNIAETLHFLLCGASNGSCQEQIDLVPRSPQELYELTSLICELMPCLPKEGIFAVDTMLKKGNAQNTDGAIWQWRDDRGLWHPYNRIDSRIIEVQINEDTGTARAIQRKPNPLANTNTSGYSELKKDDARAQLMKEDPELAKSFIKTLFGVLYEVYSSSAGPAVRHKCLRAILRIIYFADAELLKDVLKNHAVSSHIASMLSSQDLKIVVGALQMAEILMQKLPDIFSVYFRREGVMHQVKHLAESESLLTSPPKACTNGSGSLGSTTSVSSGTATAATNAAADLGSPSLQHSRDDSLDLSPQGRLSDVLKRKRLPKRGPRRPKYSPPRDDDKVDNQAKSPTTTQSPKSSFLASLNPKTWGRLSAQSNSNNIEPARTAGVSGLARAASKDTISNNREKIKGWIKEQAHKFVERYFSSENMDGSNPALNVLQRLCAATEQLNLQVDGGAECLVEIRSIVSESDVSSFEIQHSGFVKQLLLYLTSKSEKDAVSREIRLKRFLHVFFSSPLPGEEPIGRVEPVGNAPLLALVHKMNNCLSQMEQFPVKVHDFPSGNGTGGSFSLNRGSQALKFFNTHQLKCQLQRHPDCANVKQWKGGPVKIDPLALVQAIERYLVVRGYGRVREDDEDSDDDGSDEEIDESLAAQFLNSGNVRHRLQFYIGEHLLPYNMTVYQAVRQFSIQAEDERESTDDESNPLGRAGIWTKTHTIWYKPVREDEESNKDCVGGKRGRAQTAPTKTSPRNAKKHDELWHDGVCPSVSNPLEVYLISSPPENITFEDPSLDVILLLRVLHAISRYWYYLYDNAMCKEIIPTSEFINSKLTAKANRQLQDPLVIMTGNIPTWLTELGKTCPFFFPFDTRQMLFYVTAFDRDRAMQRLLDTNPEINQSDSQDSRVAPRLDRKKRTVNREELLKQAESVMQDLGSSRAMLEIQYENEVGTGLGPTLEFYALVSQELQRADLGLWRGEEVTLSNPKGSQEGTKYIQNLQGLFALPFGRTAKPAHIAKVKMKFRFLGKLMAKAIMDFRLVDLPLGLPFYKWMLRQETSLTSHDLFDIDPVVARSVYHLEDIVRQKKRLEQDKSQTKESLQYALETLTMNGCSVEDLGLDFTLPGFPNIELKKGGKDIPVTIHNLEEYLRLVIFWALNEGVSRQFDSFRDGFESVFPLSHLQYFYPEELDQLLCGSKADTWDAKTLMECCRPDHGYTHDSRAVKFLFEILSSFDNEQQRLFLQFVTGSPRLPVGGFRSLNPPLTIVRKTFESTENPDDFLPSVMTCVNYLKLPDYSSIDIMREKLLIAAREGQQSFHLS

Target ORF information:

RefSeq Version	XM_022409667.1
Organism	Canis lupus familiaris(dog)
Definition	Canis lupus familiaris thyroid hormone receptor interactor 12 (TRIP12), transcript variant X7, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_022409667.1

ORF Insert Sequence:

ATGTCCAACC GGCCTAATAA CAATCCAGGG GGGTCACTGC GACGTTCACA GAGGAACACT 
GCCGGGGCCC AACCACAAGA CGACTCGATA GGAGGAAGGT CACATTTAGG GCAGGCAAAA 
CATAAGGGAT ATAGCCCTCC TGAGAGTAGA AAATCTAATT CTAAGGCACC CAAAGTGCAG 
TCTAATACTA CTTCTGAACT GTCAAGGGGA CACCTTTCTA AAAGAAGCTG CAGTTCATCA 
TCTGCTGTCA TAGTTCCACA ACCAGAGGAT CCAGACAGAG CCAATACTTC AGAAAGGCAA 
AAAACGGGGC AGGTGCCTAA GAAAGACAAT TCTCGAGGAG TGAAACGCAG TGCTAGTCCA 
GACTACAACA GGACCAATTC TCCTAGCTCT GCCAAAAAAC CAAAAGCACT TCAGCATACT 
GAATCTCCCT CAGAAACAAA TAAGCCACAT AGTAAGTCGA AGAAGAGACA TTTAGACCAG 
GAGCAACAAC TGAAATCTGC ACAGTCGCCA TCAACAAGTA AGGCTCACAC CAGAAAGAGT 
GGGGCCACCG GCGGCTCACG GAGTCAGAAA AGAAAGAGGA CAGAGAGTTC TTGTCTAAAG 
AGTGGTTCTG TGTCTGAGTC AACTGGTGCC GAAGAGAGAT CTGCAAAACC CACCAAGCTG 
GCTTCAAAAT CCGCCGCCTC GGCCAAAGCT GGGTGCAGCA CCATCACTGA TTCTTCCTCC 
GCTGCCTCTA CTTCCTCCTC ATCTTCTGCT GTCGCCTCGG CCTCCTCCAC TGTACCACCA 
GGGGCCAGGG TGAAACAAGG AAAAGACCAG AGCAAGGCCA GGCGTTCCCG TTCAGCATCC 
AGTCCCAGTC CCAGAAGAAG TAGCAGGGAA AAGGAACAGA GTAAAACTGG TGGCTCTTCA 
AAATTCGATT GGGCTGCTCG TTTCAGCCCT AAAGTTAGCC TTCCTAAAAC CAAACTGTCT 
CTTCCAGGGT CTTCTAAGTC AGAGACATCA AAACCTGGAC CTTCTGGATT ACAGGCCAAA 
TTAGCAAGTT TAAGAAAATC TACCAAGAAG CGCAGTGAAT CCCCACCTGC TGAGCTCCCC 
AGTTTGAGGC GGAGCACACG CCAAAAGACC ACGGGCTCCT GTGCTAGTGC CAGTCGGCGA 
GGCTCTGGCC TGGGCAAAAG AGGAGCAGCT GAAGCTCGTC GACAGGAGAA GATGGCGGAC 
CCTGAAAGCA GCCAGGAGAC CGTGAATTCT TCAGCTGCAC GGACAGACGA AACTCCCCAA 
GGAGCTGCAG CCTCTAGTTC TGTTGCAGGG GCTGTGGGCA TGACCACCTC TGGGGAGAGT 
GAATCCGATG ATTCTGAGAT GGGACGACTA CAAGCTTTGT TAGAGGCCAG GGGTCTTCCT 
CCTCATCTGT TTGGCCCTCT CGGTCCTCGG ATGTCACAAC TTTTCCATAG AACAATTGGA 
AGCGGAGCTA GCTCTAAGGC CCAGCAGCTT CTGCAAGGAC TACAAGCAAG TGATGAAAGC 
CAGCAACTCC AGGCAGTTAT TGAAATGTGT CAGTTATTGG TTATGGGAAA TGAGGAGACG 
CTGGGAGGGT TTCCGGTCAA GAGTGTCGTT CCAGCTTTGA TAACATTACT GCAGATGGAG 
CATAATTTCG ATATTATGAA CCATGCTTGT CGAGCCTTAA CATATATGAT GGAAGCACTT 
CCTCGGTCAT CTGCTGTTGT AGTAGATGCA ATTCCTGTCT TTTTAGAAAA GCTGCAAGTT 
ATTCAGTGTA TTGATGTCGC AGAGCAGGCC CTGACTGCCT TGGAGATGTT ATCACGCAGA 
CACAGTAAAG CCATTCTACA GGCGGGTGGT TTGGCAGACT GCTTGCTGTA TCTAGAATTC 
TTCAGCATAA ATGCCCAAAG AAATGCACTA GCAATTGCAG CCAATTGCTG CCAGAGTATC 
ACGCCAGATG AATTTCATTT TGTGGCAGAT TCCCTCCCGT TGCTTACCCA AAGGCTAACC 
CATCAGGACA AAAAGTCAGT TGAAAGCACT TGCCTTTGTT TTGCACGTCT AGTGGACAAC 
TTCCAACATG AGGAGAATTT ACTCCAGCAG GTTGCCTCCA AAGATCTGCT TACGAATGTT 
CAACAACTCT TGGTAGTGAC TCCACCCATT TTAAGTTCTG GGATGTTTAT AATGGTGGTT 
CGCATGTTTT CTCTGATGTG TTCCAACTGT CCAACTTTAG CTGTTCAACT TATGAAGCAA 
AACATTGCAG AAACACTTCA CTTTCTCCTG TGTGGTGCCT CTAATGGAAG TTGTCAGGAA 
CAGATTGATC TTGTTCCACG AAGTCCACAA GAACTGTATG AACTGACATC TCTGATTTGC 
GAACTTATGC CATGTTTACC AAAAGAAGGC ATTTTTGCAG TTGATACCAT GCTGAAGAAG 
GGAAATGCGC AGAACACAGA CGGTGCGATT TGGCAGTGGC GTGATGACCG TGGCCTTTGG 
CATCCCTATA ACAGGATTGA CAGCCGGATC ATTGAGGTAC AAATCAATGA GGACACGGGA 
ACAGCACGTG CCATTCAGAG AAAACCTAAC CCCTTAGCCA ATACTAACAC TAGTGGATAT 
TCAGAGTTAA AGAAGGATGA TGCTCGAGCA CAGCTTATGA AAGAGGATCC GGAACTGGCT 
AAGTCTTTTA TTAAGACATT ATTTGGTGTT CTTTATGAAG TGTATAGTTC CTCAGCAGGA 
CCTGCGGTCA GACATAAGTG CCTTAGAGCA ATTCTTAGGA TAATTTATTT TGCGGATGCT 
GAACTTCTGA AGGATGTCCT GAAAAATCAT GCTGTTTCAA GTCACATTGC TTCCATGCTG 
TCAAGCCAAG ACCTGAAGAT AGTAGTTGGA GCACTTCAGA TGGCAGAAAT CTTAATGCAG 
AAGTTACCTG ATATTTTTAG TGTTTACTTC AGAAGAGAAG GTGTAATGCA TCAAGTAAAA 
CACTTAGCAG AATCAGAGTC TTTGTTGACA AGTCCACCAA AGGCATGTAC GAATGGGTCA 
GGATCCCTGG GATCCACGAC GTCAGTCAGC AGTGGAACAG CCACGGCCGC CACCAATGCT 
GCAGCTGACC TGGGGTCCCC CAGCTTGCAG CACAGCAGAG ATGACTCTTT AGATCTGAGC 
CCTCAAGGTC GATTAAGTGA TGTTCTAAAG AGAAAACGGC TGCCAAAACG AGGGCCAAGA 
AGGCCAAAGT ATTCACCTCC AAGAGATGAT GACAAAGTAG ACAATCAAGC TAAAAGCCCT 
ACCACTACTC AGTCACCTAA ATCTTCTTTC CTGGCAAGCT TGAATCCAAA AACGTGGGGA 
AGGTTAAGCG CACAGTCCAA CAGCAACAAC ATCGAACCAG CACGAACTGC GGGAGTCAGT 
GGTCTTGCCA GGGCCGCCTC CAAGGACACC ATATCCAATA ACAGAGAAAA AATTAAAGGT 
TGGATTAAGG AACAGGCGCA TAAATTTGTA GAGCGTTATT TCAGTTCTGA GAACATGGAT 
GGAAGCAACC CTGCACTGAA TGTCCTTCAG AGACTTTGTG CTGCAACTGA ACAACTCAAC 
CTCCAGGTGG ATGGTGGAGC TGAGTGCCTT GTAGAAATCC GTAGCATAGT CTCAGAGTCT 
GATGTTTCAT CATTTGAAAT CCAACATAGT GGATTTGTGA AGCAGCTGTT ACTTTATTTG 
ACATCTAAAA GTGAAAAGGA TGCTGTGAGC CGAGAGATCA GATTAAAGAG ATTCCTTCAC 
GTATTTTTTT CTTCACCACT TCCTGGAGAA GAGCCCATTG GAAGAGTAGA ACCAGTGGGT 
AATGCGCCTT TGTTGGCATT AGTTCACAAG ATGAACAACT GCCTCAGCCA GATGGAACAG 
TTTCCAGTCA AAGTACATGA CTTCCCCAGT GGGAACGGGA CAGGAGGCAG CTTTTCTCTC 
AACAGAGGAT CACAGGCTTT AAAATTTTTC AACACACACC AGTTAAAATG CCAGTTACAA 
AGACATCCAG ACTGTGCAAA TGTGAAGCAG TGGAAGGGCG GGCCGGTCAA GATTGATCCC 
CTGGCTTTGG TACAAGCTAT TGAGAGATAC CTTGTAGTTA GAGGGTATGG AAGAGTAAGA 
GAAGATGATG AAGACAGTGA TGATGATGGC TCTGATGAGG AAATAGACGA GTCTCTGGCC 
GCTCAGTTCT TAAATTCCGG AAACGTAAGG CACAGGCTAC AGTTTTATAT TGGAGAACAT 
TTGCTACCAT ATAACATGAC TGTATATCAG GCAGTCCGGC AGTTCAGTAT ACAAGCTGAG 
GATGAGCGGG AGTCCACAGA TGATGAGAGC AACCCTCTGG GAAGAGCTGG TATTTGGACA 
AAGACTCATA CAATATGGTA CAAACCTGTG AGAGAGGATG AAGAAAGTAA TAAAGATTGT 
GTTGGTGGTA AAAGAGGAAG AGCCCAAACG GCTCCAACAA AAACATCCCC TAGAAATGCA 
AAAAAGCATG ATGAGTTATG GCATGATGGA GTATGCCCAT CAGTATCCAA TCCTTTAGAA 
GTTTACCTCA TCTCCTCGCC ACCTGAGAAT ATAACTTTTG AAGACCCGTC CTTAGATGTG 
ATTCTTCTTC TAAGAGTTTT ACATGCCATC AGCCGATACT GGTATTACTT GTATGATAAT 
GCAATGTGCA AGGAGATTAT TCCAACTAGT GAATTTATTA ACAGTAAGTT GACAGCAAAA 
GCAAATAGGC AGCTTCAAGA TCCTTTAGTA ATCATGACAG GAAATATCCC AACGTGGCTT 
ACTGAACTAG GAAAAACCTG CCCATTTTTC TTCCCTTTTG ATACCCGGCA AATGCTTTTT 
TACGTAACTG CATTTGATCG GGACCGAGCA ATGCAAAGAT TACTTGATAC CAACCCAGAA 
ATCAACCAGT CGGATTCTCA AGATAGCAGA GTTGCACCTA GATTGGATAG AAAAAAACGT 
ACTGTGAACA GAGAGGAGCT GCTGAAACAA GCGGAGTCTG TAATGCAGGA TCTCGGTAGC 
TCACGGGCCA TGTTAGAAAT CCAGTATGAA AATGAGGTTG GTACAGGTCT TGGGCCTACA 
CTGGAGTTTT ATGCACTTGT ATCTCAGGAA CTTCAGAGAG CTGACTTGGG TCTCTGGAGA 
GGTGAAGAAG TGACTCTCAG CAATCCAAAA GGAAGCCAAG AAGGGACCAA GTATATTCAA 
AACCTCCAGG GCCTGTTTGC ACTTCCCTTT GGTAGGACAG CTAAGCCAGC CCATATCGCA 
AAGGTTAAAA TGAAGTTTCG CTTCTTAGGA AAATTAATGG CCAAGGCTAT CATGGATTTC 
AGATTGGTGG ACCTTCCCCT TGGTTTACCC TTTTATAAGT GGATGCTAAG GCAAGAAACT 
TCACTGACGT CGCATGATTT GTTTGACATT GACCCAGTTG TAGCCAGATC AGTGTATCAC 
CTAGAAGACA TTGTCAGACA GAAGAAAAGA CTTGAGCAAG ATAAATCCCA GACCAAAGAG 
AGTCTACAAT ATGCATTAGA AACCCTGACT ATGAATGGCT GCTCAGTTGA AGATCTAGGA 
TTGGATTTCA CGCTGCCAGG GTTTCCCAAC ATTGAACTGA AGAAAGGAGG AAAGGATATA 
CCAGTTACCA TCCACAATTT AGAGGAGTAT CTAAGACTGG TTATATTCTG GGCACTAAAT 
GAAGGCGTTT CCAGGCAATT TGATTCCTTC AGAGATGGAT TTGAATCTGT CTTCCCACTT 
AGTCATCTTC AGTACTTCTA CCCGGAAGAA CTGGATCAGC TCTTGTGCGG CAGTAAAGCA 
GACACGTGGG ATGCGAAGAC ACTGATGGAG TGCTGCAGGC CGGACCACGG TTATACTCAT 
GACAGTCGGG CTGTGAAGTT CTTGTTTGAG ATTCTCAGTA GTTTTGATAA TGAGCAGCAG 
AGGTTGTTTC TCCAGTTTGT GACAGGTAGC CCAAGATTGC CTGTTGGAGG CTTCCGGAGT 
CTGAATCCAC CTCTGACAAT TGTCCGGAAG ACCTTCGAGT CCACGGAGAA CCCCGACGAC 
TTCTTGCCCT CTGTAATGAC TTGTGTGAAC TACCTTAAGT TGCCGGACTA CTCGAGCATT 
GACATCATGC GTGAGAAACT GTTGATTGCA GCGAGAGAAG GGCAGCAGTC ATTCCATCTT 
TCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TRIP12 ( XM_022409668.1 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_022265376.1 Canis lupus familiaris thyroid hormone receptor interactor 12 (TRIP12), transcript variant X8, mRNA.

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CloneID	OCe25765
Clone ID Related Accession (Same CDS sequence)	XM_022409668.1 , XM_005635805.2
Accession Version	XM_022409668.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6123bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-09-04
Organism	Canis lupus familiaris(dog)
Product	E3 ubiquitin-protein ligase TRIP12 isoform X6
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006607.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCCAACC GGCCTAATAA CAATCCAGGG GGGTCACTGC GACGTTCACA GAGGAACACT 
GCCGGGGCCC AACCACAAGA CGACTCGATA GGAGGAAGGT CACATTTAGG GCAGGCAAAA 
CATAAGGGAT ATAGCCCTCC TGAGAGTAGA AAATCTAATT CTAAGGCACC CAAAGTGCAG 
TCTAATACTA CTTCTGAACT GTCAAGGGGA CACCTTTCTA AAAGAAGCTG CAGTTCATCA 
TCTGCTGTCA TAGTTCCACA ACCAGAGGAT CCAGACAGAG CCAATACTTC AGAAAGGCAA 
AAAACGGGGC AGGTGCCTAA GAAAGACAAT TCTCGAGGAG TGAAACGCAG TGCTAGTCCA 
GACTACAACA GGACCAATTC TCCTAGCTCT GCCAAAAAAC CAAAAGCACT TCAGCATACT 
GAATCTCCCT CAGAAACAAA TAAGCCACAT AGTAAGTCGA AGAAGAGACA TTTAGACCAG 
GAGCAACAAC TGAAATCTGC ACAGTCGCCA TCAACAAGTA AGGCTCACAC CAGAAAGAGT 
GGGGCCACCG GCGGCTCACG GAGTCAGAAA AGAAAGAGGA CAGAGAGTTC TTGTCTAAAG 
AGTGGTTCTG TGTCTGAGTC AACTGGTGCC GAAGAGAGAT CTGCAAAACC CACCAAGCTG 
GCTTCAAAAT CCGCCGCCTC GGCCAAAGCT GGGTGCAGCA CCATCACTGA TTCTTCCTCC 
GCTGCCTCTA CTTCCTCCTC ATCTTCTGCT GTCGCCTCGG CCTCCTCCAC TGTACCACCA 
GGGGCCAGGG TGAAACAAGG AAAAGACCAG AGCAAGGCCA GGCGTTCCCG TTCAGCATCC 
AGTCCCAGTC CCAGAAGAAG TAGCAGGGAA AAGGAACAGA GTAAAACTGG TGGCTCTTCA 
AAATTCGATT GGGCTGCTCG TTTCAGCCCT AAAGTTAGCC TTCCTAAAAC CAAACTGTCT 
CTTCCAGGGT CTTCTAAGTC AGAGACATCA AAACCTGGAC CTTCTGGATT ACAGGCCAAA 
TTAGCAAGTT TAAGAAAATC TACCAAGAAG CGCAGTGAAT CCCCACCTGC TGAGCTCCCC 
AGTTTGAGGC GGAGCACACG CCAAAAGACC ACGGGCTCCT GTGCTAGTGC CAGTCGGCGA 
GGCTCTGGCC TGGGCAAAAG AGGAGCAGCT GAAGCTCGTC GACAGGAGAA GATGGCGGAC 
CCTGAAAGCA GCCAGGAGAC CGTGAATTCT TCAGCTGCAC GGACAGACGA AACTCCCCAA 
GGAGCTGCAG CCTCTAGTTC TGTTGCAGGG GCTGTGGGCA TGACCACCTC TGGGGAGAGT 
GAATCCGATG ATTCTGAGAT GGGACGACTA CAAGCTTTGT TAGAGGCCAG GGGTCTTCCT 
CCTCATCTGT TTGGCCCTCT CGGTCCTCGG ATGTCACAAC TTTTCCATAG AACAATTGGA 
AGCGGAGCTA GCTCTAAGGC CCAGCAGCTT CTGCAAGGAC TACAAGCAAG TGATGAAAGC 
CAGCAACTCC AGGCAGTTAT TGAAATGTGT CAGTTATTGG TTATGGGAAA TGAGGAGACG 
CTGGGAGGGT TTCCGGTCAA GAGTGTCGTT CCAGCTTTGA TAACATTACT GCAGATGGAG 
CATAATTTCG ATATTATGAA CCATGCTTGT CGAGCCTTAA CATATATGAT GGAAGCACTT 
CCTCGGTCAT CTGCTGTTGT AGTAGATGCA ATTCCTGTCT TTTTAGAAAA GCTGCAAGTT 
ATTCAGTGTA TTGATGTCGC AGAGCAGGCC CTGACTGCCT TGGAGATGTT ATCACGCAGA 
CACAGTAAAG CCATTCTACA GGCGGGTGGT TTGGCAGACT GCTTGCTGTA TCTAGAATTC 
TTCAGCATAA ATGCCCAAAG AAATGCACTA GCAATTGCAG CCAATTGCTG CCAGAGTATC 
ACGCCAGATG AATTTCATTT TGTGGCAGAT TCCCTCCCGT TGCTTACCCA AAGGCTAACC 
CATCAGGACA AAAAGTCAGT TGAAAGCACT TGCCTTTGTT TTGCACGTCT AGTGGACAAC 
TTCCAACATG AGGAGAATTT ACTCCAGCAG GTTGCCTCCA AAGATCTGCT TACGAATGTT 
CAACAACTCT TGGTAGTGAC TCCACCCATT TTAAGTTCTG GGATGTTTAT AATGGTGGTT 
CGCATGTTTT CTCTGATGTG TTCCAACTGT CCAACTTTAG CTGTTCAACT TATGAAGCAA 
AACATTGCAG AAACACTTCA CTTTCTCCTG TGTGGTGCCT CTAATGGAAG TTGTCAGGAA 
CAGATTGATC TTGTTCCACG AAGTCCACAA GAACTGTATG AACTGACATC TCTGATTTGC 
GAACTTATGC CATGTTTACC AAAAGAAGGC ATTTTTGCAG TTGATACCAT GCTGAAGAAG 
GGAAATGCGC AGAACACAGA CGGTGCGATT TGGCAGTGGC GTGATGACCG TGGCCTTTGG 
CATCCCTATA ACAGGATTGA CAGCCGGATC ATTGAGCAAA TCAATGAGGA CACGGGAACA 
GCACGTGCCA TTCAGAGAAA ACCTAACCCC TTAGCCAATA CTAACACTAG TGGATATTCA 
GAGTTAAAGA AGGATGATGC TCGAGCACAG CTTATGAAAG AGGATCCGGA ACTGGCTAAG 
TCTTTTATTA AGACATTATT TGGTGTTCTT TATGAAGTGT ATAGTTCCTC AGCAGGACCT 
GCGGTCAGAC ATAAGTGCCT TAGAGCAATT CTTAGGATAA TTTATTTTGC GGATGCTGAA 
CTTCTGAAGG ATGTCCTGAA AAATCATGCT GTTTCAAGTC ACATTGCTTC CATGCTGTCA 
AGCCAAGACC TGAAGATAGT AGTTGGAGCA CTTCAGATGG CAGAAATCTT AATGCAGAAG 
TTACCTGATA TTTTTAGTGT TTACTTCAGA AGAGAAGGTG TAATGCATCA AGTAAAACAC 
TTAGCAGAAT CAGAGTCTTT GTTGACAAGT CCACCAAAGG CATGTACGAA TGGGTCAGGA 
TCCCTGGGAT CCACGACGTC AGTCAGCAGT GGAACAGCCA CGGCCGCCAC CAATGCTGCA 
GCTGACCTGG GGTCCCCCAG CTTGCAGCAC AGCAGAGATG ACTCTTTAGA TCTGAGCCCT 
CAAGGTCGAT TAAGTGATGT TCTAAAGAGA AAACGGCTGC CAAAACGAGG GCCAAGAAGG 
CCAAAGTATT CACCTCCAAG AGATGATGAC AAAGTAGACA ATCAAGCTAA AAGCCCTACC 
ACTACTCAGT CACCTAAATC TTCTTTCCTG GCAAGCTTGA ATCCAAAAAC GTGGGGAAGG 
TTAAGCGCAC AGTCCAACAG CAACAACATC GAACCAGCAC GAACTGCGGG AGTCAGTGGT 
CTTGCCAGGG CCGCCTCCAA GGACACCATA TCCAATAACA GAGAAAAAAT TAAAGGTTGG 
ATTAAGGAAC AGGCGCATAA ATTTGTAGAG CGTTATTTCA GTTCTGAGAA CATGGATGGA 
AGCAACCCTG CACTGAATGT CCTTCAGAGA CTTTGTGCTG CAACTGAACA ACTCAACCTC 
CAGGTGGATG GTGGAGCTGA GTGCCTTGTA GAAATCCGTA GCATAGTCTC AGAGTCTGAT 
GTTTCATCAT TTGAAATCCA ACATAGTGGA TTTGTGAAGC AGCTGTTACT TTATTTGACA 
TCTAAAAGTG AAAAGGATGC TGTGAGCCGA GAGATCAGAT TAAAGAGATT CCTTCACGTA 
TTTTTTTCTT CACCACTTCC TGGAGAAGAG CCCATTGGAA GAGTAGAACC AGTGGGTAAT 
GCGCCTTTGT TGGCATTAGT TCACAAGATG AACAACTGCC TCAGCCAGAT GGAACAGTTT 
CCAGTCAAAG TACATGACTT CCCCAGTGGG AACGGGACAG GAGGCAGCTT TTCTCTCAAC 
AGAGGATCAC AGGCTTTAAA ATTTTTCAAC ACACACCAGT TAAAATGCCA GTTACAAAGA 
CATCCAGACT GTGCAAATGT GAAGCAGTGG AAGGGCGGGC CGGTCAAGAT TGATCCCCTG 
GCTTTGGTAC AAGCTATTGA GAGATACCTT GTAGTTAGAG GGTATGGAAG AGTAAGAGAA 
GATGATGAAG ACAGTGATGA TGATGGCTCT GATGAGGAAA TAGACGAGTC TCTGGCCGCT 
CAGTTCTTAA ATTCCGGAAA CGTAAGGCAC AGGCTACAGT TTTATATTGG AGAACATTTG 
CTACCATATA ACATGACTGT ATATCAGGCA GTCCGGCAGT TCAGTATACA AGCTGAGGAT 
GAGCGGGAGT CCACAGATGA TGAGAGCAAC CCTCTGGGAA GAGCTGGTAT TTGGACAAAG 
ACTCATACAA TATGGTACAA ACCTGTGAGA GAGGATGAAG AAAGTAATAA AGATTGTGTT 
GGTGGTAAAA GAGGAAGAGC CCAAACGGCT CCAACAAAAA CATCCCCTAG AAATGCAAAA 
AAGCATGATG AGTTATGGCA TGATGGAGTA TGCCCATCAG TATCCAATCC TTTAGAAGTT 
TACCTCATCT CCTCGCCACC TGAGAATATA ACTTTTGAAG ACCCGTCCTT AGATGTGATT 
CTTCTTCTAA GAGTTTTACA TGCCATCAGC CGATACTGGT ATTACTTGTA TGATAATGCA 
ATGTGCAAGG AGATTATTCC AACTAGTGAA TTTATTAACA GTAAGTTGAC AGCAAAAGCA 
AATAGGCAGC TTCAAGATCC TTTAGTAATC ATGACAGGAA ATATCCCAAC GTGGCTTACT 
GAACTAGGAA AAACCTGCCC ATTTTTCTTC CCTTTTGATA CCCGGCAAAT GCTTTTTTAC 
GTAACTGCAT TTGATCGGGA CCGAGCAATG CAAAGATTAC TTGATACCAA CCCAGAAATC 
AACCAGTCGG ATTCTCAAGA TAGCAGAGTT GCACCTAGAT TGGATAGAAA AAAACGTACT 
GTGAACAGAG AGGAGCTGCT GAAACAAGCG GAGTCTGTAA TGCAGGATCT CGGTAGCTCA 
CGGGCCATGT TAGAAATCCA GTATGAAAAT GAGGTTGGTA CAGGTCTTGG GCCTACACTG 
GAGTTTTATG CACTTGTATC TCAGGAACTT CAGAGAGCTG ACTTGGGTCT CTGGAGAGGT 
GAAGAAGTGA CTCTCAGCAA TCCAAAAGGA AGCCAAGAAG GGACCAAGTA TATTCAAAAC 
CTCCAGGGCC TGTTTGCACT TCCCTTTGGT AGGACAGCTA AGCCAGCCCA TATCGCAAAG 
GTTAAAATGA AGTTTCGCTT CTTAGGAAAA TTAATGGCCA AGGCTATCAT GGATTTCAGA 
TTGGTGGACC TTCCCCTTGG TTTACCCTTT TATAAGTGGA TGCTAAGGCA AGAAACTTCA 
CTGACGTCGC ATGATTTGTT TGACATTGAC CCAGTTGTAG CCAGATCAGT GTATCACCTA 
GAAGACATTG TCAGACAGAA GAAAAGACTT GAGCAAGATA AATCCCAGAC CAAAGAGAGT 
CTACAATATG CATTAGAAAC CCTGACTATG AATGGCTGCT CAGTTGAAGA TCTAGGATTG 
GATTTCACGC TGCCAGGGTT TCCCAACATT GAACTGAAGA AAGGAGGAAA GGATATACCA 
GTTACCATCC ACAATTTAGA GGAGTATCTA AGACTGGTTA TATTCTGGGC ACTAAATGAA 
GGCGTTTCCA GGCAATTTGA TTCCTTCAGA GATGGATTTG AATCTGTCTT CCCACTTAGT 
CATCTTCAGT ACTTCTACCC GGAAGAACTG GATCAGCTCT TGTGCGGCAG TAAAGCAGAC 
ACGTGGGATG CGAAGACACT GATGGAGTGC TGCAGGCCGG ACCACGGTTA TACTCATGAC 
AGTCGGGCTG TGAAGTTCTT GTTTGAGATT CTCAGTAGTT TTGATAATGA GCAGCAGAGG 
TTGTTTCTCC AGTTTGTGAC AGGTAGCCCA AGATTGCCTG TTGGAGGCTT CCGGAGTCTG 
AATCCACCTC TGACAATTGT CCGGAAGACC TTCGAGTCCA CGGAGAACCC CGACGACTTC 
TTGCCCTCTG TAATGACTTG TGTGAACTAC CTTAAGTTGC CGGACTACTC GAGCATTGAC 
ATCATGCGTG AGAAACTGTT GATTGCAGCG AGAGAAGGGC AGCAGTCATT CCATCTTTCC 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_022265376.1
CDS	1..6123
Translation	MSNRPNNNPGGSLRRSQRNTAGAQPQDDSIGGRSHLGQAKHKGYSPPESRKSNSKAPKVQSNTTSELSRGHLSKRSCSSSSAVIVPQPEDPDRANTSERQKTGQVPKKDNSRGVKRSASPDYNRTNSPSSAKKPKALQHTESPSETNKPHSKSKKRHLDQEQQLKSAQSPSTSKAHTRKSGATGGSRSQKRKRTESSCLKSGSVSESTGAEERSAKPTKLASKSAASAKAGCSTITDSSSAASTSSSSSAVASASSTVPPGARVKQGKDQSKARRSRSASSPSPRRSSREKEQSKTGGSSKFDWAARFSPKVSLPKTKLSLPGSSKSETSKPGPSGLQAKLASLRKSTKKRSESPPAELPSLRRSTRQKTTGSCASASRRGSGLGKRGAAEARRQEKMADPESSQETVNSSAARTDETPQGAAASSSVAGAVGMTTSGESESDDSEMGRLQALLEARGLPPHLFGPLGPRMSQLFHRTIGSGASSKAQQLLQGLQASDESQQLQAVIEMCQLLVMGNEETLGGFPVKSVVPALITLLQMEHNFDIMNHACRALTYMMEALPRSSAVVVDAIPVFLEKLQVIQCIDVAEQALTALEMLSRRHSKAILQAGGLADCLLYLEFFSINAQRNALAIAANCCQSITPDEFHFVADSLPLLTQRLTHQDKKSVESTCLCFARLVDNFQHEENLLQQVASKDLLTNVQQLLVVTPPILSSGMFIMVVRMFSLMCSNCPTLAVQLMKQNIAETLHFLLCGASNGSCQEQIDLVPRSPQELYELTSLICELMPCLPKEGIFAVDTMLKKGNAQNTDGAIWQWRDDRGLWHPYNRIDSRIIEQINEDTGTARAIQRKPNPLANTNTSGYSELKKDDARAQLMKEDPELAKSFIKTLFGVLYEVYSSSAGPAVRHKCLRAILRIIYFADAELLKDVLKNHAVSSHIASMLSSQDLKIVVGALQMAEILMQKLPDIFSVYFRREGVMHQVKHLAESESLLTSPPKACTNGSGSLGSTTSVSSGTATAATNAAADLGSPSLQHSRDDSLDLSPQGRLSDVLKRKRLPKRGPRRPKYSPPRDDDKVDNQAKSPTTTQSPKSSFLASLNPKTWGRLSAQSNSNNIEPARTAGVSGLARAASKDTISNNREKIKGWIKEQAHKFVERYFSSENMDGSNPALNVLQRLCAATEQLNLQVDGGAECLVEIRSIVSESDVSSFEIQHSGFVKQLLLYLTSKSEKDAVSREIRLKRFLHVFFSSPLPGEEPIGRVEPVGNAPLLALVHKMNNCLSQMEQFPVKVHDFPSGNGTGGSFSLNRGSQALKFFNTHQLKCQLQRHPDCANVKQWKGGPVKIDPLALVQAIERYLVVRGYGRVREDDEDSDDDGSDEEIDESLAAQFLNSGNVRHRLQFYIGEHLLPYNMTVYQAVRQFSIQAEDERESTDDESNPLGRAGIWTKTHTIWYKPVREDEESNKDCVGGKRGRAQTAPTKTSPRNAKKHDELWHDGVCPSVSNPLEVYLISSPPENITFEDPSLDVILLLRVLHAISRYWYYLYDNAMCKEIIPTSEFINSKLTAKANRQLQDPLVIMTGNIPTWLTELGKTCPFFFPFDTRQMLFYVTAFDRDRAMQRLLDTNPEINQSDSQDSRVAPRLDRKKRTVNREELLKQAESVMQDLGSSRAMLEIQYENEVGTGLGPTLEFYALVSQELQRADLGLWRGEEVTLSNPKGSQEGTKYIQNLQGLFALPFGRTAKPAHIAKVKMKFRFLGKLMAKAIMDFRLVDLPLGLPFYKWMLRQETSLTSHDLFDIDPVVARSVYHLEDIVRQKKRLEQDKSQTKESLQYALETLTMNGCSVEDLGLDFTLPGFPNIELKKGGKDIPVTIHNLEEYLRLVIFWALNEGVSRQFDSFRDGFESVFPLSHLQYFYPEELDQLLCGSKADTWDAKTLMECCRPDHGYTHDSRAVKFLFEILSSFDNEQQRLFLQFVTGSPRLPVGGFRSLNPPLTIVRKTFESTENPDDFLPSVMTCVNYLKLPDYSSIDIMREKLLIAAREGQQSFHLS

Target ORF information:

RefSeq Version	XM_022409668.1
Organism	Canis lupus familiaris(dog)
Definition	Canis lupus familiaris thyroid hormone receptor interactor 12 (TRIP12), transcript variant X8, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_022409668.1

ORF Insert Sequence:

ATGTCCAACC GGCCTAATAA CAATCCAGGG GGGTCACTGC GACGTTCACA GAGGAACACT 
GCCGGGGCCC AACCACAAGA CGACTCGATA GGAGGAAGGT CACATTTAGG GCAGGCAAAA 
CATAAGGGAT ATAGCCCTCC TGAGAGTAGA AAATCTAATT CTAAGGCACC CAAAGTGCAG 
TCTAATACTA CTTCTGAACT GTCAAGGGGA CACCTTTCTA AAAGAAGCTG CAGTTCATCA 
TCTGCTGTCA TAGTTCCACA ACCAGAGGAT CCAGACAGAG CCAATACTTC AGAAAGGCAA 
AAAACGGGGC AGGTGCCTAA GAAAGACAAT TCTCGAGGAG TGAAACGCAG TGCTAGTCCA 
GACTACAACA GGACCAATTC TCCTAGCTCT GCCAAAAAAC CAAAAGCACT TCAGCATACT 
GAATCTCCCT CAGAAACAAA TAAGCCACAT AGTAAGTCGA AGAAGAGACA TTTAGACCAG 
GAGCAACAAC TGAAATCTGC ACAGTCGCCA TCAACAAGTA AGGCTCACAC CAGAAAGAGT 
GGGGCCACCG GCGGCTCACG GAGTCAGAAA AGAAAGAGGA CAGAGAGTTC TTGTCTAAAG 
AGTGGTTCTG TGTCTGAGTC AACTGGTGCC GAAGAGAGAT CTGCAAAACC CACCAAGCTG 
GCTTCAAAAT CCGCCGCCTC GGCCAAAGCT GGGTGCAGCA CCATCACTGA TTCTTCCTCC 
GCTGCCTCTA CTTCCTCCTC ATCTTCTGCT GTCGCCTCGG CCTCCTCCAC TGTACCACCA 
GGGGCCAGGG TGAAACAAGG AAAAGACCAG AGCAAGGCCA GGCGTTCCCG TTCAGCATCC 
AGTCCCAGTC CCAGAAGAAG TAGCAGGGAA AAGGAACAGA GTAAAACTGG TGGCTCTTCA 
AAATTCGATT GGGCTGCTCG TTTCAGCCCT AAAGTTAGCC TTCCTAAAAC CAAACTGTCT 
CTTCCAGGGT CTTCTAAGTC AGAGACATCA AAACCTGGAC CTTCTGGATT ACAGGCCAAA 
TTAGCAAGTT TAAGAAAATC TACCAAGAAG CGCAGTGAAT CCCCACCTGC TGAGCTCCCC 
AGTTTGAGGC GGAGCACACG CCAAAAGACC ACGGGCTCCT GTGCTAGTGC CAGTCGGCGA 
GGCTCTGGCC TGGGCAAAAG AGGAGCAGCT GAAGCTCGTC GACAGGAGAA GATGGCGGAC 
CCTGAAAGCA GCCAGGAGAC CGTGAATTCT TCAGCTGCAC GGACAGACGA AACTCCCCAA 
GGAGCTGCAG CCTCTAGTTC TGTTGCAGGG GCTGTGGGCA TGACCACCTC TGGGGAGAGT 
GAATCCGATG ATTCTGAGAT GGGACGACTA CAAGCTTTGT TAGAGGCCAG GGGTCTTCCT 
CCTCATCTGT TTGGCCCTCT CGGTCCTCGG ATGTCACAAC TTTTCCATAG AACAATTGGA 
AGCGGAGCTA GCTCTAAGGC CCAGCAGCTT CTGCAAGGAC TACAAGCAAG TGATGAAAGC 
CAGCAACTCC AGGCAGTTAT TGAAATGTGT CAGTTATTGG TTATGGGAAA TGAGGAGACG 
CTGGGAGGGT TTCCGGTCAA GAGTGTCGTT CCAGCTTTGA TAACATTACT GCAGATGGAG 
CATAATTTCG ATATTATGAA CCATGCTTGT CGAGCCTTAA CATATATGAT GGAAGCACTT 
CCTCGGTCAT CTGCTGTTGT AGTAGATGCA ATTCCTGTCT TTTTAGAAAA GCTGCAAGTT 
ATTCAGTGTA TTGATGTCGC AGAGCAGGCC CTGACTGCCT TGGAGATGTT ATCACGCAGA 
CACAGTAAAG CCATTCTACA GGCGGGTGGT TTGGCAGACT GCTTGCTGTA TCTAGAATTC 
TTCAGCATAA ATGCCCAAAG AAATGCACTA GCAATTGCAG CCAATTGCTG CCAGAGTATC 
ACGCCAGATG AATTTCATTT TGTGGCAGAT TCCCTCCCGT TGCTTACCCA AAGGCTAACC 
CATCAGGACA AAAAGTCAGT TGAAAGCACT TGCCTTTGTT TTGCACGTCT AGTGGACAAC 
TTCCAACATG AGGAGAATTT ACTCCAGCAG GTTGCCTCCA AAGATCTGCT TACGAATGTT 
CAACAACTCT TGGTAGTGAC TCCACCCATT TTAAGTTCTG GGATGTTTAT AATGGTGGTT 
CGCATGTTTT CTCTGATGTG TTCCAACTGT CCAACTTTAG CTGTTCAACT TATGAAGCAA 
AACATTGCAG AAACACTTCA CTTTCTCCTG TGTGGTGCCT CTAATGGAAG TTGTCAGGAA 
CAGATTGATC TTGTTCCACG AAGTCCACAA GAACTGTATG AACTGACATC TCTGATTTGC 
GAACTTATGC CATGTTTACC AAAAGAAGGC ATTTTTGCAG TTGATACCAT GCTGAAGAAG 
GGAAATGCGC AGAACACAGA CGGTGCGATT TGGCAGTGGC GTGATGACCG TGGCCTTTGG 
CATCCCTATA ACAGGATTGA CAGCCGGATC ATTGAGCAAA TCAATGAGGA CACGGGAACA 
GCACGTGCCA TTCAGAGAAA ACCTAACCCC TTAGCCAATA CTAACACTAG TGGATATTCA 
GAGTTAAAGA AGGATGATGC TCGAGCACAG CTTATGAAAG AGGATCCGGA ACTGGCTAAG 
TCTTTTATTA AGACATTATT TGGTGTTCTT TATGAAGTGT ATAGTTCCTC AGCAGGACCT 
GCGGTCAGAC ATAAGTGCCT TAGAGCAATT CTTAGGATAA TTTATTTTGC GGATGCTGAA 
CTTCTGAAGG ATGTCCTGAA AAATCATGCT GTTTCAAGTC ACATTGCTTC CATGCTGTCA 
AGCCAAGACC TGAAGATAGT AGTTGGAGCA CTTCAGATGG CAGAAATCTT AATGCAGAAG 
TTACCTGATA TTTTTAGTGT TTACTTCAGA AGAGAAGGTG TAATGCATCA AGTAAAACAC 
TTAGCAGAAT CAGAGTCTTT GTTGACAAGT CCACCAAAGG CATGTACGAA TGGGTCAGGA 
TCCCTGGGAT CCACGACGTC AGTCAGCAGT GGAACAGCCA CGGCCGCCAC CAATGCTGCA 
GCTGACCTGG GGTCCCCCAG CTTGCAGCAC AGCAGAGATG ACTCTTTAGA TCTGAGCCCT 
CAAGGTCGAT TAAGTGATGT TCTAAAGAGA AAACGGCTGC CAAAACGAGG GCCAAGAAGG 
CCAAAGTATT CACCTCCAAG AGATGATGAC AAAGTAGACA ATCAAGCTAA AAGCCCTACC 
ACTACTCAGT CACCTAAATC TTCTTTCCTG GCAAGCTTGA ATCCAAAAAC GTGGGGAAGG 
TTAAGCGCAC AGTCCAACAG CAACAACATC GAACCAGCAC GAACTGCGGG AGTCAGTGGT 
CTTGCCAGGG CCGCCTCCAA GGACACCATA TCCAATAACA GAGAAAAAAT TAAAGGTTGG 
ATTAAGGAAC AGGCGCATAA ATTTGTAGAG CGTTATTTCA GTTCTGAGAA CATGGATGGA 
AGCAACCCTG CACTGAATGT CCTTCAGAGA CTTTGTGCTG CAACTGAACA ACTCAACCTC 
CAGGTGGATG GTGGAGCTGA GTGCCTTGTA GAAATCCGTA GCATAGTCTC AGAGTCTGAT 
GTTTCATCAT TTGAAATCCA ACATAGTGGA TTTGTGAAGC AGCTGTTACT TTATTTGACA 
TCTAAAAGTG AAAAGGATGC TGTGAGCCGA GAGATCAGAT TAAAGAGATT CCTTCACGTA 
TTTTTTTCTT CACCACTTCC TGGAGAAGAG CCCATTGGAA GAGTAGAACC AGTGGGTAAT 
GCGCCTTTGT TGGCATTAGT TCACAAGATG AACAACTGCC TCAGCCAGAT GGAACAGTTT 
CCAGTCAAAG TACATGACTT CCCCAGTGGG AACGGGACAG GAGGCAGCTT TTCTCTCAAC 
AGAGGATCAC AGGCTTTAAA ATTTTTCAAC ACACACCAGT TAAAATGCCA GTTACAAAGA 
CATCCAGACT GTGCAAATGT GAAGCAGTGG AAGGGCGGGC CGGTCAAGAT TGATCCCCTG 
GCTTTGGTAC AAGCTATTGA GAGATACCTT GTAGTTAGAG GGTATGGAAG AGTAAGAGAA 
GATGATGAAG ACAGTGATGA TGATGGCTCT GATGAGGAAA TAGACGAGTC TCTGGCCGCT 
CAGTTCTTAA ATTCCGGAAA CGTAAGGCAC AGGCTACAGT TTTATATTGG AGAACATTTG 
CTACCATATA ACATGACTGT ATATCAGGCA GTCCGGCAGT TCAGTATACA AGCTGAGGAT 
GAGCGGGAGT CCACAGATGA TGAGAGCAAC CCTCTGGGAA GAGCTGGTAT TTGGACAAAG 
ACTCATACAA TATGGTACAA ACCTGTGAGA GAGGATGAAG AAAGTAATAA AGATTGTGTT 
GGTGGTAAAA GAGGAAGAGC CCAAACGGCT CCAACAAAAA CATCCCCTAG AAATGCAAAA 
AAGCATGATG AGTTATGGCA TGATGGAGTA TGCCCATCAG TATCCAATCC TTTAGAAGTT 
TACCTCATCT CCTCGCCACC TGAGAATATA ACTTTTGAAG ACCCGTCCTT AGATGTGATT 
CTTCTTCTAA GAGTTTTACA TGCCATCAGC CGATACTGGT ATTACTTGTA TGATAATGCA 
ATGTGCAAGG AGATTATTCC AACTAGTGAA TTTATTAACA GTAAGTTGAC AGCAAAAGCA 
AATAGGCAGC TTCAAGATCC TTTAGTAATC ATGACAGGAA ATATCCCAAC GTGGCTTACT 
GAACTAGGAA AAACCTGCCC ATTTTTCTTC CCTTTTGATA CCCGGCAAAT GCTTTTTTAC 
GTAACTGCAT TTGATCGGGA CCGAGCAATG CAAAGATTAC TTGATACCAA CCCAGAAATC 
AACCAGTCGG ATTCTCAAGA TAGCAGAGTT GCACCTAGAT TGGATAGAAA AAAACGTACT 
GTGAACAGAG AGGAGCTGCT GAAACAAGCG GAGTCTGTAA TGCAGGATCT CGGTAGCTCA 
CGGGCCATGT TAGAAATCCA GTATGAAAAT GAGGTTGGTA CAGGTCTTGG GCCTACACTG 
GAGTTTTATG CACTTGTATC TCAGGAACTT CAGAGAGCTG ACTTGGGTCT CTGGAGAGGT 
GAAGAAGTGA CTCTCAGCAA TCCAAAAGGA AGCCAAGAAG GGACCAAGTA TATTCAAAAC 
CTCCAGGGCC TGTTTGCACT TCCCTTTGGT AGGACAGCTA AGCCAGCCCA TATCGCAAAG 
GTTAAAATGA AGTTTCGCTT CTTAGGAAAA TTAATGGCCA AGGCTATCAT GGATTTCAGA 
TTGGTGGACC TTCCCCTTGG TTTACCCTTT TATAAGTGGA TGCTAAGGCA AGAAACTTCA 
CTGACGTCGC ATGATTTGTT TGACATTGAC CCAGTTGTAG CCAGATCAGT GTATCACCTA 
GAAGACATTG TCAGACAGAA GAAAAGACTT GAGCAAGATA AATCCCAGAC CAAAGAGAGT 
CTACAATATG CATTAGAAAC CCTGACTATG AATGGCTGCT CAGTTGAAGA TCTAGGATTG 
GATTTCACGC TGCCAGGGTT TCCCAACATT GAACTGAAGA AAGGAGGAAA GGATATACCA 
GTTACCATCC ACAATTTAGA GGAGTATCTA AGACTGGTTA TATTCTGGGC ACTAAATGAA 
GGCGTTTCCA GGCAATTTGA TTCCTTCAGA GATGGATTTG AATCTGTCTT CCCACTTAGT 
CATCTTCAGT ACTTCTACCC GGAAGAACTG GATCAGCTCT TGTGCGGCAG TAAAGCAGAC 
ACGTGGGATG CGAAGACACT GATGGAGTGC TGCAGGCCGG ACCACGGTTA TACTCATGAC 
AGTCGGGCTG TGAAGTTCTT GTTTGAGATT CTCAGTAGTT TTGATAATGA GCAGCAGAGG 
TTGTTTCTCC AGTTTGTGAC AGGTAGCCCA AGATTGCCTG TTGGAGGCTT CCGGAGTCTG 
AATCCACCTC TGACAATTGT CCGGAAGACC TTCGAGTCCA CGGAGAACCC CGACGACTTC 
TTGCCCTCTG TAATGACTTG TGTGAACTAC CTTAAGTTGC CGGACTACTC GAGCATTGAC 
ATCATGCGTG AGAAACTGTT GATTGCAGCG AGAGAAGGGC AGCAGTCATT CCATCTTTCC 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TRIP12 ( XM_005635805.2 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_005635862.1 Canis lupus familiaris thyroid hormone receptor interactor 12 (TRIP12), transcript variant X6, mRNA.

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CloneID	OCe25765
Clone ID Related Accession (Same CDS sequence)	XM_022409668.1 , XM_005635805.2
Accession Version	XM_005635805.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6123bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-09-16
Organism	Canis lupus familiaris(Dog)
Product	E3 ubiquitin-protein ligase TRIP12 isoform X5
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726103.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 17, 2015 this sequence version replaced XM_005635805.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCCAACC GGCCTAATAA CAATCCAGGG GGGTCACTGC GACGTTCACA GAGGAACACT 
GCCGGGGCCC AACCACAAGA CGACTCGATA GGAGGAAGGT CACATTTAGG GCAGGCAAAA 
CATAAGGGAT ATAGCCCTCC TGAGAGTAGA AAATCTAATT CTAAGGCACC CAAAGTGCAG 
TCTAATACTA CTTCTGAACT GTCAAGGGGA CACCTTTCTA AAAGAAGCTG CAGTTCATCA 
TCTGCTGTCA TAGTTCCACA ACCAGAGGAT CCAGACAGAG CCAATACTTC AGAAAGGCAA 
AAAACGGGGC AGGTGCCTAA GAAAGACAAT TCTCGAGGAG TGAAACGCAG TGCTAGTCCA 
GACTACAACA GGACCAATTC TCCTAGCTCT GCCAAAAAAC CAAAAGCACT TCAGCATACT 
GAATCTCCCT CAGAAACAAA TAAGCCACAT AGTAAGTCGA AGAAGAGACA TTTAGACCAG 
GAGCAACAAC TGAAATCTGC ACAGTCGCCA TCAACAAGTA AGGCTCACAC CAGAAAGAGT 
GGGGCCACCG GCGGCTCACG GAGTCAGAAA AGAAAGAGGA CAGAGAGTTC TTGTCTAAAG 
AGTGGTTCTG TGTCTGAGTC AACTGGTGCC GAAGAGAGAT CTGCAAAACC CACCAAGCTG 
GCTTCAAAAT CCGCCGCCTC GGCCAAAGCT GGGTGCAGCA CCATCACTGA TTCTTCCTCC 
GCTGCCTCTA CTTCCTCCTC ATCTTCTGCT GTCGCCTCGG CCTCCTCCAC TGTACCACCA 
GGGGCCAGGG TGAAACAAGG AAAAGACCAG AGCAAGGCCA GGCGTTCCCG TTCAGCATCC 
AGTCCCAGTC CCAGAAGAAG TAGCAGGGAA AAGGAACAGA GTAAAACTGG TGGCTCTTCA 
AAATTCGATT GGGCTGCTCG TTTCAGCCCT AAAGTTAGCC TTCCTAAAAC CAAACTGTCT 
CTTCCAGGGT CTTCTAAGTC AGAGACATCA AAACCTGGAC CTTCTGGATT ACAGGCCAAA 
TTAGCAAGTT TAAGAAAATC TACCAAGAAG CGCAGTGAAT CCCCACCTGC TGAGCTCCCC 
AGTTTGAGGC GGAGCACACG CCAAAAGACC ACGGGCTCCT GTGCTAGTGC CAGTCGGCGA 
GGCTCTGGCC TGGGCAAAAG AGGAGCAGCT GAAGCTCGTC GACAGGAGAA GATGGCGGAC 
CCTGAAAGCA GCCAGGAGAC CGTGAATTCT TCAGCTGCAC GGACAGACGA AACTCCCCAA 
GGAGCTGCAG CCTCTAGTTC TGTTGCAGGG GCTGTGGGCA TGACCACCTC TGGGGAGAGT 
GAATCCGATG ATTCTGAGAT GGGACGACTA CAAGCTTTGT TAGAGGCCAG GGGTCTTCCT 
CCTCATCTGT TTGGCCCTCT CGGTCCTCGG ATGTCACAAC TTTTCCATAG AACAATTGGA 
AGCGGAGCTA GCTCTAAGGC CCAGCAGCTT CTGCAAGGAC TACAAGCAAG TGATGAAAGC 
CAGCAACTCC AGGCAGTTAT TGAAATGTGT CAGTTATTGG TTATGGGAAA TGAGGAGACG 
CTGGGAGGGT TTCCGGTCAA GAGTGTCGTT CCAGCTTTGA TAACATTACT GCAGATGGAG 
CATAATTTCG ATATTATGAA CCATGCTTGT CGAGCCTTAA CATATATGAT GGAAGCACTT 
CCTCGGTCAT CTGCTGTTGT AGTAGATGCA ATTCCTGTCT TTTTAGAAAA GCTGCAAGTT 
ATTCAGTGTA TTGATGTCGC AGAGCAGGCC CTGACTGCCT TGGAGATGTT ATCACGCAGA 
CACAGTAAAG CCATTCTACA GGCGGGTGGT TTGGCAGACT GCTTGCTGTA TCTAGAATTC 
TTCAGCATAA ATGCCCAAAG AAATGCACTA GCAATTGCAG CCAATTGCTG CCAGAGTATC 
ACGCCAGATG AATTTCATTT TGTGGCAGAT TCCCTCCCGT TGCTTACCCA AAGGCTAACC 
CATCAGGACA AAAAGTCAGT TGAAAGCACT TGCCTTTGTT TTGCACGTCT AGTGGACAAC 
TTCCAACATG AGGAGAATTT ACTCCAGCAG GTTGCCTCCA AAGATCTGCT TACGAATGTT 
CAACAACTCT TGGTAGTGAC TCCACCCATT TTAAGTTCTG GGATGTTTAT AATGGTGGTT 
CGCATGTTTT CTCTGATGTG TTCCAACTGT CCAACTTTAG CTGTTCAACT TATGAAGCAA 
AACATTGCAG AAACACTTCA CTTTCTCCTG TGTGGTGCCT CTAATGGAAG TTGTCAGGAA 
CAGATTGATC TTGTTCCACG AAGTCCACAA GAACTGTATG AACTGACATC TCTGATTTGC 
GAACTTATGC CATGTTTACC AAAAGAAGGC ATTTTTGCAG TTGATACCAT GCTGAAGAAG 
GGAAATGCGC AGAACACAGA CGGTGCGATT TGGCAGTGGC GTGATGACCG TGGCCTTTGG 
CATCCCTATA ACAGGATTGA CAGCCGGATC ATTGAGCAAA TCAATGAGGA CACGGGAACA 
GCACGTGCCA TTCAGAGAAA ACCTAACCCC TTAGCCAATA CTAACACTAG TGGATATTCA 
GAGTTAAAGA AGGATGATGC TCGAGCACAG CTTATGAAAG AGGATCCGGA ACTGGCTAAG 
TCTTTTATTA AGACATTATT TGGTGTTCTT TATGAAGTGT ATAGTTCCTC AGCAGGACCT 
GCGGTCAGAC ATAAGTGCCT TAGAGCAATT CTTAGGATAA TTTATTTTGC GGATGCTGAA 
CTTCTGAAGG ATGTCCTGAA AAATCATGCT GTTTCAAGTC ACATTGCTTC CATGCTGTCA 
AGCCAAGACC TGAAGATAGT AGTTGGAGCA CTTCAGATGG CAGAAATCTT AATGCAGAAG 
TTACCTGATA TTTTTAGTGT TTACTTCAGA AGAGAAGGTG TAATGCATCA AGTAAAACAC 
TTAGCAGAAT CAGAGTCTTT GTTGACAAGT CCACCAAAGG CATGTACGAA TGGGTCAGGA 
TCCCTGGGAT CCACGACGTC AGTCAGCAGT GGAACAGCCA CGGCCGCCAC CAATGCTGCA 
GCTGACCTGG GGTCCCCCAG CTTGCAGCAC AGCAGAGATG ACTCTTTAGA TCTGAGCCCT 
CAAGGTCGAT TAAGTGATGT TCTAAAGAGA AAACGGCTGC CAAAACGAGG GCCAAGAAGG 
CCAAAGTATT CACCTCCAAG AGATGATGAC AAAGTAGACA ATCAAGCTAA AAGCCCTACC 
ACTACTCAGT CACCTAAATC TTCTTTCCTG GCAAGCTTGA ATCCAAAAAC GTGGGGAAGG 
TTAAGCGCAC AGTCCAACAG CAACAACATC GAACCAGCAC GAACTGCGGG AGTCAGTGGT 
CTTGCCAGGG CCGCCTCCAA GGACACCATA TCCAATAACA GAGAAAAAAT TAAAGGTTGG 
ATTAAGGAAC AGGCGCATAA ATTTGTAGAG CGTTATTTCA GTTCTGAGAA CATGGATGGA 
AGCAACCCTG CACTGAATGT CCTTCAGAGA CTTTGTGCTG CAACTGAACA ACTCAACCTC 
CAGGTGGATG GTGGAGCTGA GTGCCTTGTA GAAATCCGTA GCATAGTCTC AGAGTCTGAT 
GTTTCATCAT TTGAAATCCA ACATAGTGGA TTTGTGAAGC AGCTGTTACT TTATTTGACA 
TCTAAAAGTG AAAAGGATGC TGTGAGCCGA GAGATCAGAT TAAAGAGATT CCTTCACGTA 
TTTTTTTCTT CACCACTTCC TGGAGAAGAG CCCATTGGAA GAGTAGAACC AGTGGGTAAT 
GCGCCTTTGT TGGCATTAGT TCACAAGATG AACAACTGCC TCAGCCAGAT GGAACAGTTT 
CCAGTCAAAG TACATGACTT CCCCAGTGGG AACGGGACAG GAGGCAGCTT TTCTCTCAAC 
AGAGGATCAC AGGCTTTAAA ATTTTTCAAC ACACACCAGT TAAAATGCCA GTTACAAAGA 
CATCCAGACT GTGCAAATGT GAAGCAGTGG AAGGGCGGGC CGGTCAAGAT TGATCCCCTG 
GCTTTGGTAC AAGCTATTGA GAGATACCTT GTAGTTAGAG GGTATGGAAG AGTAAGAGAA 
GATGATGAAG ACAGTGATGA TGATGGCTCT GATGAGGAAA TAGACGAGTC TCTGGCCGCT 
CAGTTCTTAA ATTCCGGAAA CGTAAGGCAC AGGCTACAGT TTTATATTGG AGAACATTTG 
CTACCATATA ACATGACTGT ATATCAGGCA GTCCGGCAGT TCAGTATACA AGCTGAGGAT 
GAGCGGGAGT CCACAGATGA TGAGAGCAAC CCTCTGGGAA GAGCTGGTAT TTGGACAAAG 
ACTCATACAA TATGGTACAA ACCTGTGAGA GAGGATGAAG AAAGTAATAA AGATTGTGTT 
GGTGGTAAAA GAGGAAGAGC CCAAACGGCT CCAACAAAAA CATCCCCTAG AAATGCAAAA 
AAGCATGATG AGTTATGGCA TGATGGAGTA TGCCCATCAG TATCCAATCC TTTAGAAGTT 
TACCTCATCT CCTCGCCACC TGAGAATATA ACTTTTGAAG ACCCGTCCTT AGATGTGATT 
CTTCTTCTAA GAGTTTTACA TGCCATCAGC CGATACTGGT ATTACTTGTA TGATAATGCA 
ATGTGCAAGG AGATTATTCC AACTAGTGAA TTTATTAACA GTAAGTTGAC AGCAAAAGCA 
AATAGGCAGC TTCAAGATCC TTTAGTAATC ATGACAGGAA ATATCCCAAC GTGGCTTACT 
GAACTAGGAA AAACCTGCCC ATTTTTCTTC CCTTTTGATA CCCGGCAAAT GCTTTTTTAC 
GTAACTGCAT TTGATCGGGA CCGAGCAATG CAAAGATTAC TTGATACCAA CCCAGAAATC 
AACCAGTCGG ATTCTCAAGA TAGCAGAGTT GCACCTAGAT TGGATAGAAA AAAACGTACT 
GTGAACAGAG AGGAGCTGCT GAAACAAGCG GAGTCTGTAA TGCAGGATCT CGGTAGCTCA 
CGGGCCATGT TAGAAATCCA GTATGAAAAT GAGGTTGGTA CAGGTCTTGG GCCTACACTG 
GAGTTTTATG CACTTGTATC TCAGGAACTT CAGAGAGCTG ACTTGGGTCT CTGGAGAGGT 
GAAGAAGTGA CTCTCAGCAA TCCAAAAGGA AGCCAAGAAG GGACCAAGTA TATTCAAAAC 
CTCCAGGGCC TGTTTGCACT TCCCTTTGGT AGGACAGCTA AGCCAGCCCA TATCGCAAAG 
GTTAAAATGA AGTTTCGCTT CTTAGGAAAA TTAATGGCCA AGGCTATCAT GGATTTCAGA 
TTGGTGGACC TTCCCCTTGG TTTACCCTTT TATAAGTGGA TGCTAAGGCA AGAAACTTCA 
CTGACGTCGC ATGATTTGTT TGACATTGAC CCAGTTGTAG CCAGATCAGT GTATCACCTA 
GAAGACATTG TCAGACAGAA GAAAAGACTT GAGCAAGATA AATCCCAGAC CAAAGAGAGT 
CTACAATATG CATTAGAAAC CCTGACTATG AATGGCTGCT CAGTTGAAGA TCTAGGATTG 
GATTTCACGC TGCCAGGGTT TCCCAACATT GAACTGAAGA AAGGAGGAAA GGATATACCA 
GTTACCATCC ACAATTTAGA GGAGTATCTA AGACTGGTTA TATTCTGGGC ACTAAATGAA 
GGCGTTTCCA GGCAATTTGA TTCCTTCAGA GATGGATTTG AATCTGTCTT CCCACTTAGT 
CATCTTCAGT ACTTCTACCC GGAAGAACTG GATCAGCTCT TGTGCGGCAG TAAAGCAGAC 
ACGTGGGATG CGAAGACACT GATGGAGTGC TGCAGGCCGG ACCACGGTTA TACTCATGAC 
AGTCGGGCTG TGAAGTTCTT GTTTGAGATT CTCAGTAGTT TTGATAATGA GCAGCAGAGG 
TTGTTTCTCC AGTTTGTGAC AGGTAGCCCA AGATTGCCTG TTGGAGGCTT CCGGAGTCTG 
AATCCACCTC TGACAATTGT CCGGAAGACC TTCGAGTCCA CGGAGAACCC CGACGACTTC 
TTGCCCTCTG TAATGACTTG TGTGAACTAC CTTAAGTTGC CGGACTACTC GAGCATTGAC 
ATCATGCGTG AGAAACTGTT GATTGCAGCG AGAGAAGGGC AGCAGTCATT CCATCTTTCC 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005635862.1
CDS	188..6310
Translation	MSNRPNNNPGGSLRRSQRNTAGAQPQDDSIGGRSHLGQAKHKGYSPPESRKSNSKAPKVQSNTTSELSRGHLSKRSCSSSSAVIVPQPEDPDRANTSERQKTGQVPKKDNSRGVKRSASPDYNRTNSPSSAKKPKALQHTESPSETNKPHSKSKKRHLDQEQQLKSAQSPSTSKAHTRKSGATGGSRSQKRKRTESSCLKSGSVSESTGAEERSAKPTKLASKSAASAKAGCSTITDSSSAASTSSSSSAVASASSTVPPGARVKQGKDQSKARRSRSASSPSPRRSSREKEQSKTGGSSKFDWAARFSPKVSLPKTKLSLPGSSKSETSKPGPSGLQAKLASLRKSTKKRSESPPAELPSLRRSTRQKTTGSCASASRRGSGLGKRGAAEARRQEKMADPESSQETVNSSAARTDETPQGAAASSSVAGAVGMTTSGESESDDSEMGRLQALLEARGLPPHLFGPLGPRMSQLFHRTIGSGASSKAQQLLQGLQASDESQQLQAVIEMCQLLVMGNEETLGGFPVKSVVPALITLLQMEHNFDIMNHACRALTYMMEALPRSSAVVVDAIPVFLEKLQVIQCIDVAEQALTALEMLSRRHSKAILQAGGLADCLLYLEFFSINAQRNALAIAANCCQSITPDEFHFVADSLPLLTQRLTHQDKKSVESTCLCFARLVDNFQHEENLLQQVASKDLLTNVQQLLVVTPPILSSGMFIMVVRMFSLMCSNCPTLAVQLMKQNIAETLHFLLCGASNGSCQEQIDLVPRSPQELYELTSLICELMPCLPKEGIFAVDTMLKKGNAQNTDGAIWQWRDDRGLWHPYNRIDSRIIEQINEDTGTARAIQRKPNPLANTNTSGYSELKKDDARAQLMKEDPELAKSFIKTLFGVLYEVYSSSAGPAVRHKCLRAILRIIYFADAELLKDVLKNHAVSSHIASMLSSQDLKIVVGALQMAEILMQKLPDIFSVYFRREGVMHQVKHLAESESLLTSPPKACTNGSGSLGSTTSVSSGTATAATNAAADLGSPSLQHSRDDSLDLSPQGRLSDVLKRKRLPKRGPRRPKYSPPRDDDKVDNQAKSPTTTQSPKSSFLASLNPKTWGRLSAQSNSNNIEPARTAGVSGLARAASKDTISNNREKIKGWIKEQAHKFVERYFSSENMDGSNPALNVLQRLCAATEQLNLQVDGGAECLVEIRSIVSESDVSSFEIQHSGFVKQLLLYLTSKSEKDAVSREIRLKRFLHVFFSSPLPGEEPIGRVEPVGNAPLLALVHKMNNCLSQMEQFPVKVHDFPSGNGTGGSFSLNRGSQALKFFNTHQLKCQLQRHPDCANVKQWKGGPVKIDPLALVQAIERYLVVRGYGRVREDDEDSDDDGSDEEIDESLAAQFLNSGNVRHRLQFYIGEHLLPYNMTVYQAVRQFSIQAEDERESTDDESNPLGRAGIWTKTHTIWYKPVREDEESNKDCVGGKRGRAQTAPTKTSPRNAKKHDELWHDGVCPSVSNPLEVYLISSPPENITFEDPSLDVILLLRVLHAISRYWYYLYDNAMCKEIIPTSEFINSKLTAKANRQLQDPLVIMTGNIPTWLTELGKTCPFFFPFDTRQMLFYVTAFDRDRAMQRLLDTNPEINQSDSQDSRVAPRLDRKKRTVNREELLKQAESVMQDLGSSRAMLEIQYENEVGTGLGPTLEFYALVSQELQRADLGLWRGEEVTLSNPKGSQEGTKYIQNLQGLFALPFGRTAKPAHIAKVKMKFRFLGKLMAKAIMDFRLVDLPLGLPFYKWMLRQETSLTSHDLFDIDPVVARSVYHLEDIVRQKKRLEQDKSQTKESLQYALETLTMNGCSVEDLGLDFTLPGFPNIELKKGGKDIPVTIHNLEEYLRLVIFWALNEGVSRQFDSFRDGFESVFPLSHLQYFYPEELDQLLCGSKADTWDAKTLMECCRPDHGYTHDSRAVKFLFEILSSFDNEQQRLFLQFVTGSPRLPVGGFRSLNPPLTIVRKTFESTENPDDFLPSVMTCVNYLKLPDYSSIDIMREKLLIAAREGQQSFHLS

Target ORF information:

RefSeq Version	XM_005635805.2
Organism	Canis lupus familiaris(Dog)
Definition	Canis lupus familiaris thyroid hormone receptor interactor 12 (TRIP12), transcript variant X6, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005635805.2

ORF Insert Sequence:

ATGTCCAACC GGCCTAATAA CAATCCAGGG GGGTCACTGC GACGTTCACA GAGGAACACT 
GCCGGGGCCC AACCACAAGA CGACTCGATA GGAGGAAGGT CACATTTAGG GCAGGCAAAA 
CATAAGGGAT ATAGCCCTCC TGAGAGTAGA AAATCTAATT CTAAGGCACC CAAAGTGCAG 
TCTAATACTA CTTCTGAACT GTCAAGGGGA CACCTTTCTA AAAGAAGCTG CAGTTCATCA 
TCTGCTGTCA TAGTTCCACA ACCAGAGGAT CCAGACAGAG CCAATACTTC AGAAAGGCAA 
AAAACGGGGC AGGTGCCTAA GAAAGACAAT TCTCGAGGAG TGAAACGCAG TGCTAGTCCA 
GACTACAACA GGACCAATTC TCCTAGCTCT GCCAAAAAAC CAAAAGCACT TCAGCATACT 
GAATCTCCCT CAGAAACAAA TAAGCCACAT AGTAAGTCGA AGAAGAGACA TTTAGACCAG 
GAGCAACAAC TGAAATCTGC ACAGTCGCCA TCAACAAGTA AGGCTCACAC CAGAAAGAGT 
GGGGCCACCG GCGGCTCACG GAGTCAGAAA AGAAAGAGGA CAGAGAGTTC TTGTCTAAAG 
AGTGGTTCTG TGTCTGAGTC AACTGGTGCC GAAGAGAGAT CTGCAAAACC CACCAAGCTG 
GCTTCAAAAT CCGCCGCCTC GGCCAAAGCT GGGTGCAGCA CCATCACTGA TTCTTCCTCC 
GCTGCCTCTA CTTCCTCCTC ATCTTCTGCT GTCGCCTCGG CCTCCTCCAC TGTACCACCA 
GGGGCCAGGG TGAAACAAGG AAAAGACCAG AGCAAGGCCA GGCGTTCCCG TTCAGCATCC 
AGTCCCAGTC CCAGAAGAAG TAGCAGGGAA AAGGAACAGA GTAAAACTGG TGGCTCTTCA 
AAATTCGATT GGGCTGCTCG TTTCAGCCCT AAAGTTAGCC TTCCTAAAAC CAAACTGTCT 
CTTCCAGGGT CTTCTAAGTC AGAGACATCA AAACCTGGAC CTTCTGGATT ACAGGCCAAA 
TTAGCAAGTT TAAGAAAATC TACCAAGAAG CGCAGTGAAT CCCCACCTGC TGAGCTCCCC 
AGTTTGAGGC GGAGCACACG CCAAAAGACC ACGGGCTCCT GTGCTAGTGC CAGTCGGCGA 
GGCTCTGGCC TGGGCAAAAG AGGAGCAGCT GAAGCTCGTC GACAGGAGAA GATGGCGGAC 
CCTGAAAGCA GCCAGGAGAC CGTGAATTCT TCAGCTGCAC GGACAGACGA AACTCCCCAA 
GGAGCTGCAG CCTCTAGTTC TGTTGCAGGG GCTGTGGGCA TGACCACCTC TGGGGAGAGT 
GAATCCGATG ATTCTGAGAT GGGACGACTA CAAGCTTTGT TAGAGGCCAG GGGTCTTCCT 
CCTCATCTGT TTGGCCCTCT CGGTCCTCGG ATGTCACAAC TTTTCCATAG AACAATTGGA 
AGCGGAGCTA GCTCTAAGGC CCAGCAGCTT CTGCAAGGAC TACAAGCAAG TGATGAAAGC 
CAGCAACTCC AGGCAGTTAT TGAAATGTGT CAGTTATTGG TTATGGGAAA TGAGGAGACG 
CTGGGAGGGT TTCCGGTCAA GAGTGTCGTT CCAGCTTTGA TAACATTACT GCAGATGGAG 
CATAATTTCG ATATTATGAA CCATGCTTGT CGAGCCTTAA CATATATGAT GGAAGCACTT 
CCTCGGTCAT CTGCTGTTGT AGTAGATGCA ATTCCTGTCT TTTTAGAAAA GCTGCAAGTT 
ATTCAGTGTA TTGATGTCGC AGAGCAGGCC CTGACTGCCT TGGAGATGTT ATCACGCAGA 
CACAGTAAAG CCATTCTACA GGCGGGTGGT TTGGCAGACT GCTTGCTGTA TCTAGAATTC 
TTCAGCATAA ATGCCCAAAG AAATGCACTA GCAATTGCAG CCAATTGCTG CCAGAGTATC 
ACGCCAGATG AATTTCATTT TGTGGCAGAT TCCCTCCCGT TGCTTACCCA AAGGCTAACC 
CATCAGGACA AAAAGTCAGT TGAAAGCACT TGCCTTTGTT TTGCACGTCT AGTGGACAAC 
TTCCAACATG AGGAGAATTT ACTCCAGCAG GTTGCCTCCA AAGATCTGCT TACGAATGTT 
CAACAACTCT TGGTAGTGAC TCCACCCATT TTAAGTTCTG GGATGTTTAT AATGGTGGTT 
CGCATGTTTT CTCTGATGTG TTCCAACTGT CCAACTTTAG CTGTTCAACT TATGAAGCAA 
AACATTGCAG AAACACTTCA CTTTCTCCTG TGTGGTGCCT CTAATGGAAG TTGTCAGGAA 
CAGATTGATC TTGTTCCACG AAGTCCACAA GAACTGTATG AACTGACATC TCTGATTTGC 
GAACTTATGC CATGTTTACC AAAAGAAGGC ATTTTTGCAG TTGATACCAT GCTGAAGAAG 
GGAAATGCGC AGAACACAGA CGGTGCGATT TGGCAGTGGC GTGATGACCG TGGCCTTTGG 
CATCCCTATA ACAGGATTGA CAGCCGGATC ATTGAGCAAA TCAATGAGGA CACGGGAACA 
GCACGTGCCA TTCAGAGAAA ACCTAACCCC TTAGCCAATA CTAACACTAG TGGATATTCA 
GAGTTAAAGA AGGATGATGC TCGAGCACAG CTTATGAAAG AGGATCCGGA ACTGGCTAAG 
TCTTTTATTA AGACATTATT TGGTGTTCTT TATGAAGTGT ATAGTTCCTC AGCAGGACCT 
GCGGTCAGAC ATAAGTGCCT TAGAGCAATT CTTAGGATAA TTTATTTTGC GGATGCTGAA 
CTTCTGAAGG ATGTCCTGAA AAATCATGCT GTTTCAAGTC ACATTGCTTC CATGCTGTCA 
AGCCAAGACC TGAAGATAGT AGTTGGAGCA CTTCAGATGG CAGAAATCTT AATGCAGAAG 
TTACCTGATA TTTTTAGTGT TTACTTCAGA AGAGAAGGTG TAATGCATCA AGTAAAACAC 
TTAGCAGAAT CAGAGTCTTT GTTGACAAGT CCACCAAAGG CATGTACGAA TGGGTCAGGA 
TCCCTGGGAT CCACGACGTC AGTCAGCAGT GGAACAGCCA CGGCCGCCAC CAATGCTGCA 
GCTGACCTGG GGTCCCCCAG CTTGCAGCAC AGCAGAGATG ACTCTTTAGA TCTGAGCCCT 
CAAGGTCGAT TAAGTGATGT TCTAAAGAGA AAACGGCTGC CAAAACGAGG GCCAAGAAGG 
CCAAAGTATT CACCTCCAAG AGATGATGAC AAAGTAGACA ATCAAGCTAA AAGCCCTACC 
ACTACTCAGT CACCTAAATC TTCTTTCCTG GCAAGCTTGA ATCCAAAAAC GTGGGGAAGG 
TTAAGCGCAC AGTCCAACAG CAACAACATC GAACCAGCAC GAACTGCGGG AGTCAGTGGT 
CTTGCCAGGG CCGCCTCCAA GGACACCATA TCCAATAACA GAGAAAAAAT TAAAGGTTGG 
ATTAAGGAAC AGGCGCATAA ATTTGTAGAG CGTTATTTCA GTTCTGAGAA CATGGATGGA 
AGCAACCCTG CACTGAATGT CCTTCAGAGA CTTTGTGCTG CAACTGAACA ACTCAACCTC 
CAGGTGGATG GTGGAGCTGA GTGCCTTGTA GAAATCCGTA GCATAGTCTC AGAGTCTGAT 
GTTTCATCAT TTGAAATCCA ACATAGTGGA TTTGTGAAGC AGCTGTTACT TTATTTGACA 
TCTAAAAGTG AAAAGGATGC TGTGAGCCGA GAGATCAGAT TAAAGAGATT CCTTCACGTA 
TTTTTTTCTT CACCACTTCC TGGAGAAGAG CCCATTGGAA GAGTAGAACC AGTGGGTAAT 
GCGCCTTTGT TGGCATTAGT TCACAAGATG AACAACTGCC TCAGCCAGAT GGAACAGTTT 
CCAGTCAAAG TACATGACTT CCCCAGTGGG AACGGGACAG GAGGCAGCTT TTCTCTCAAC 
AGAGGATCAC AGGCTTTAAA ATTTTTCAAC ACACACCAGT TAAAATGCCA GTTACAAAGA 
CATCCAGACT GTGCAAATGT GAAGCAGTGG AAGGGCGGGC CGGTCAAGAT TGATCCCCTG 
GCTTTGGTAC AAGCTATTGA GAGATACCTT GTAGTTAGAG GGTATGGAAG AGTAAGAGAA 
GATGATGAAG ACAGTGATGA TGATGGCTCT GATGAGGAAA TAGACGAGTC TCTGGCCGCT 
CAGTTCTTAA ATTCCGGAAA CGTAAGGCAC AGGCTACAGT TTTATATTGG AGAACATTTG 
CTACCATATA ACATGACTGT ATATCAGGCA GTCCGGCAGT TCAGTATACA AGCTGAGGAT 
GAGCGGGAGT CCACAGATGA TGAGAGCAAC CCTCTGGGAA GAGCTGGTAT TTGGACAAAG 
ACTCATACAA TATGGTACAA ACCTGTGAGA GAGGATGAAG AAAGTAATAA AGATTGTGTT 
GGTGGTAAAA GAGGAAGAGC CCAAACGGCT CCAACAAAAA CATCCCCTAG AAATGCAAAA 
AAGCATGATG AGTTATGGCA TGATGGAGTA TGCCCATCAG TATCCAATCC TTTAGAAGTT 
TACCTCATCT CCTCGCCACC TGAGAATATA ACTTTTGAAG ACCCGTCCTT AGATGTGATT 
CTTCTTCTAA GAGTTTTACA TGCCATCAGC CGATACTGGT ATTACTTGTA TGATAATGCA 
ATGTGCAAGG AGATTATTCC AACTAGTGAA TTTATTAACA GTAAGTTGAC AGCAAAAGCA 
AATAGGCAGC TTCAAGATCC TTTAGTAATC ATGACAGGAA ATATCCCAAC GTGGCTTACT 
GAACTAGGAA AAACCTGCCC ATTTTTCTTC CCTTTTGATA CCCGGCAAAT GCTTTTTTAC 
GTAACTGCAT TTGATCGGGA CCGAGCAATG CAAAGATTAC TTGATACCAA CCCAGAAATC 
AACCAGTCGG ATTCTCAAGA TAGCAGAGTT GCACCTAGAT TGGATAGAAA AAAACGTACT 
GTGAACAGAG AGGAGCTGCT GAAACAAGCG GAGTCTGTAA TGCAGGATCT CGGTAGCTCA 
CGGGCCATGT TAGAAATCCA GTATGAAAAT GAGGTTGGTA CAGGTCTTGG GCCTACACTG 
GAGTTTTATG CACTTGTATC TCAGGAACTT CAGAGAGCTG ACTTGGGTCT CTGGAGAGGT 
GAAGAAGTGA CTCTCAGCAA TCCAAAAGGA AGCCAAGAAG GGACCAAGTA TATTCAAAAC 
CTCCAGGGCC TGTTTGCACT TCCCTTTGGT AGGACAGCTA AGCCAGCCCA TATCGCAAAG 
GTTAAAATGA AGTTTCGCTT CTTAGGAAAA TTAATGGCCA AGGCTATCAT GGATTTCAGA 
TTGGTGGACC TTCCCCTTGG TTTACCCTTT TATAAGTGGA TGCTAAGGCA AGAAACTTCA 
CTGACGTCGC ATGATTTGTT TGACATTGAC CCAGTTGTAG CCAGATCAGT GTATCACCTA 
GAAGACATTG TCAGACAGAA GAAAAGACTT GAGCAAGATA AATCCCAGAC CAAAGAGAGT 
CTACAATATG CATTAGAAAC CCTGACTATG AATGGCTGCT CAGTTGAAGA TCTAGGATTG 
GATTTCACGC TGCCAGGGTT TCCCAACATT GAACTGAAGA AAGGAGGAAA GGATATACCA 
GTTACCATCC ACAATTTAGA GGAGTATCTA AGACTGGTTA TATTCTGGGC ACTAAATGAA 
GGCGTTTCCA GGCAATTTGA TTCCTTCAGA GATGGATTTG AATCTGTCTT CCCACTTAGT 
CATCTTCAGT ACTTCTACCC GGAAGAACTG GATCAGCTCT TGTGCGGCAG TAAAGCAGAC 
ACGTGGGATG CGAAGACACT GATGGAGTGC TGCAGGCCGG ACCACGGTTA TACTCATGAC 
AGTCGGGCTG TGAAGTTCTT GTTTGAGATT CTCAGTAGTT TTGATAATGA GCAGCAGAGG 
TTGTTTCTCC AGTTTGTGAC AGGTAGCCCA AGATTGCCTG TTGGAGGCTT CCGGAGTCTG 
AATCCACCTC TGACAATTGT CCGGAAGACC TTCGAGTCCA CGGAGAACCC CGACGACTTC 
TTGCCCTCTG TAATGACTTG TGTGAACTAC CTTAAGTTGC CGGACTACTC GAGCATTGAC 
ATCATGCGTG AGAAACTGTT GATTGCAGCG AGAGAAGGGC AGCAGTCATT CCATCTTTCC 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TRIP12 ( XM_005635807.1 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_005635864.1 Canis lupus familiaris thyroid hormone receptor interactor 12 (TRIP12), transcript variant X7, mRNA.

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CloneID	OCe25767
Clone ID Related Accession (Same CDS sequence)	XM_005635807.1
Accession Version	XM_005635807.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6111bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-09-16
Organism	Canis lupus familiaris(Dog)
Product	E3 ubiquitin-protein ligase TRIP12 isoform X6
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726103.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCACATT TAGGGCAGGC AAAACATAAG GGATATAGCC CTCCTGAGAG TAGAAAATCT 
AATTCTAAGG CACCCAAAGT GCAGTCTAAT ACTACTTCTG AACTGTCAAG GGGACACCTT 
TCTAAAAGAA GCTGCAGTTC ATCATCTGCT GTCATAGTTC CACAACCAGA GGATCCAGAC 
AGAGCCAATA CTTCAGAAAG GCAAAAAACG GGGCAGGTGC CTAAGAAAGA CAATTCTCGA 
GGAGTGAAAC GCAGTGCTAG TCCAGACTAC AACAGGACCA ATTCTCCTAG CTCTGCCAAA 
AAACCAAAAG CACTTCAGCA TACTGAATCT CCCTCAGAAA CAAATAAGCC ACATAGTAAG 
TCGAAGAAGA GACATTTAGA CCAGGAGCAA CAACTGAAAT CTGCACAGTC GCCATCAACA 
AGTAAGGCTC ACACCAGAAA GAGTGGGGCC ACCGGCGGCT CACGGAGTCA GAAAAGAAAG 
AGGACAGAGA GTTCTTGTCT AAAGAGTGGT TCTGTGTCTG AGTCAACTGG TGCCGAAGAG 
AGATCTGCAA AACCCACCAA GCTGGCTTCA AAATCCGCCG CCTCGGCCAA AGCTGGGTGC 
AGCACCATCA CTGATTCTTC CTCCGCTGCC TCTACTTCCT CCTCATCTTC TGCTGTCGCC 
TCGGCCTCCT CCACTGTACC ACCAGGGGCC AGGGTGAAAC AAGGAAAAGA CCAGAGCAAG 
GCCAGGCGTT CCCGTTCAGC ATCCAGTCCC AGTCCCAGAA GAAGTAGCAG GGAAAAGGAA 
CAGAGTAAAA CTGGTGGCTC TTCAAAATTC GATTGGGCTG CTCGTTTCAG CCCTAAAGTT 
AGCCTTCCTA AAACCAAACT GTCTCTTCCA GGGTCTTCTA AGTCAGAGAC ATCAAAACCT 
GGACCTTCTG GATTACAGGC CAAATTAGCA AGTTTAAGAA AATCTACCAA GAAGCGCAGT 
GAATCCCCAC CTGCTGAGCT CCCCAGTTTG AGGCGGAGCA CACGCCAAAA GACCACGGGC 
TCCTGTGCTA GTGCCAGTCG GCGAGGCTCT GGCCTGGGCA AAAGAGGAGC AGCTGAAGCT 
CGTCGACAGG AGAAGATGGC GGACCCTGAA AGCAGCCAGG AGACCGTGAA TTCTTCAGCT 
GCACGGACAG ACGAAACTCC CCAAGGAGCT GCAGCCTCTA GTTCTGTTGC AGGGGCTGTG 
GGCATGACCA CCTCTGGGGA GAGTGAATCC GATGATTCTG AGATGGGACG ACTACAAGCT 
TTGTTAGAGG CCAGGGGTCT TCCTCCTCAT CTGTTTGGCC CTCTCGGTCC TCGGATGTCA 
CAACTTTTCC ATAGAACAAT TGGAAGCGGA GCTAGCTCTA AGGCCCAGCA GCTTCTGCAA 
GGACTACAAG CAAGTGATGA AAGCCAGCAA CTCCAGGCAG TTATTGAAAT GTGTCAGTTA 
TTGGTTATGG GAAATGAGGA GACGCTGGGA GGGTTTCCGG TCAAGAGTGT CGTTCCAGCT 
TTGATAACAT TACTGCAGAT GGAGCATAAT TTCGATATTA TGAACCATGC TTGTCGAGCC 
TTAACATATA TGATGGAAGC ACTTCCTCGG TCATCTGCTG TTGTAGTAGA TGCAATTCCT 
GTCTTTTTAG AAAAGCTGCA AGTTATTCAG TGTATTGATG TCGCAGAGCA GGCCCTGACT 
GCCTTGGAGA TGTTATCACG CAGACACAGT AAAGCCATTC TACAGGCGGG TGGTTTGGCA 
GACTGCTTGC TGTATCTAGA ATTCTTCAGC ATAAATGCCC AAAGAAATGC ACTAGCAATT 
GCAGCCAATT GCTGCCAGAG TATCACGCCA GATGAATTTC ATTTTGTGGC AGATTCCCTC 
CCGTTGCTTA CCCAAAGGCT AACCCATCAG GACAAAAAGT CAGTTGAAAG CACTTGCCTT 
TGTTTTGCAC GTCTAGTGGA CAACTTCCAA CATGAGGAGA ATTTACTCCA GCAGGTTGCC 
TCCAAAGATC TGCTTACGAA TGTTCAACAA CTCTTGGTAG TGACTCCACC CATTTTAAGT 
TCTGGGATGT TTATAATGGT GGTTCGCATG TTTTCTCTGA TGTGTTCCAA CTGTCCAACT 
TTAGCTGTTC AACTTATGAA GCAAAACATT GCAGAAACAC TTCACTTTCT CCTGTGTGGT 
GCCTCTAATG GAAGTTGTCA GGAACAGATT GATCTTGTTC CACGAAGTCC ACAAGAACTG 
TATGAACTGA CATCTCTGAT TTGCGAACTT ATGCCATGTT TACCAAAAGA AGGCATTTTT 
GCAGTTGATA CCATGCTGAA GAAGGGAAAT GCGCAGAACA CAGACGGTGC GATTTGGCAG 
TGGCGTGATG ACCGTGGCCT TTGGCATCCC TATAACAGGA TTGACAGCCG GATCATTGAG 
GTAGCAGCCC ATCAGGTCGG TGAGGATGAG ATAAGCTTGT CCACTCTGGG ACGAGTTTAT 
ACTATTGATT TTAATTCTAT GCAGCAAATC AATGAGGACA CGGGAACAGC ACGTGCCATT 
CAGAGAAAAC CTAACCCCTT AGCCAATACT AACACTAGTG GATATTCAGA GTTAAAGAAG 
GATGATGCTC GAGCACAGCT TATGAAAGAG GATCCGGAAC TGGCTAAGTC TTTTATTAAG 
ACATTATTTG GTGTTCTTTA TGAAGTGTAT AGTTCCTCAG CAGGACCTGC GGTCAGACAT 
AAGTGCCTTA GAGCAATTCT TAGGATAATT TATTTTGCGG ATGCTGAACT TCTGAAGGAT 
GTCCTGAAAA ATCATGCTGT TTCAAGTCAC ATTGCTTCCA TGCTGTCAAG CCAAGACCTG 
AAGATAGTAG TTGGAGCACT TCAGATGGCA GAAATCTTAA TGCAGAAGTT ACCTGATATT 
TTTAGTGTTT ACTTCAGAAG AGAAGGTGTA ATGCATCAAG TAAAACACTT AGCAGAATCA 
GAGTCTTTGT TGACAAGTCC ACCAAAGGCA TGTACGAATG GGTCAGGATC CCTGGGATCC 
ACGACGTCAG TCAGCAGTGG AACAGCCACG GCCGCCACCA ATGCTGCAGC TGACCTGGGG 
TCCCCCAGCT TGCAGCACAG CAGAGATGAC TCTTTAGATC TGAGCCCTCA AGGTCGATTA 
AGTGATGTTC TAAAGAGAAA ACGGCTGCCA AAACGAGGGC CAAGAAGGCC AAAGTATTCA 
CCTCCAAGAG ATGATGACAA AGTAGACAAT CAAGCTAAAA GCCCTACCAC TACTCAGTCA 
CCTAAATCTT CTTTCCTGGC AAGCTTGAAT CCAAAAACGT GGGGAAGGTT AAGCGCACAG 
TCCAACAGCA ACAACATCGA ACCAGCACGA ACTGCGGGAG TCAGTGGTCT TGCCAGGGCC 
GCCTCCAAGG ACACCATATC CAATAACAGA GAAAAAATTA AAGGTTGGAT TAAGGAACAG 
GCGCATAAAT TTGTAGAGCG TTATTTCAGT TCTGAGAACA TGGATGGAAG CAACCCTGCA 
CTGAATGTCC TTCAGAGACT TTGTGCTGCA ACTGAACAAC TCAACCTCCA GGTGGATGGT 
GGAGCTGAGT GCCTTGTAGA AATCCGTAGC ATAGTCTCAG AGTCTGATGT TTCATCATTT 
GAAATCCAAC ATAGTGGATT TGTGAAGCAG CTGTTACTTT ATTTGACATC TAAAAGTGAA 
AAGGATGCTG TGAGCCGAGA GATCAGATTA AAGAGATTCC TTCACGTATT TTTTTCTTCA 
CCACTTCCTG GAGAAGAGCC CATTGGAAGA GTAGAACCAG TGGGTAATGC GCCTTTGTTG 
GCATTAGTTC ACAAGATGAA CAACTGCCTC AGCCAGATGG AACAGTTTCC AGTCAAAGTA 
CATGACTTCC CCAGTGGGAA CGGGACAGGA GGCAGCTTTT CTCTCAACAG AGGATCACAG 
GCTTTAAAAT TTTTCAACAC ACACCAGTTA AAATGCCAGT TACAAAGACA TCCAGACTGT 
GCAAATGTGA AGCAGTGGAA GGGCGGGCCG GTCAAGATTG ATCCCCTGGC TTTGGTACAA 
GCTATTGAGA GATACCTTGT AGTTAGAGGG TATGGAAGAG TAAGAGAAGA TGATGAAGAC 
AGTGATGATG ATGGCTCTGA TGAGGAAATA GACGAGTCTC TGGCCGCTCA GTTCTTAAAT 
TCCGGAAACG TAAGGCACAG GCTACAGTTT TATATTGGAG AACATTTGCT ACCATATAAC 
ATGACTGTAT ATCAGGCAGT CCGGCAGTTC AGTATACAAG CTGAGGATGA GCGGGAGTCC 
ACAGATGATG AGAGCAACCC TCTGGGAAGA GCTGGTATTT GGACAAAGAC TCATACAATA 
TGGTACAAAC CTGTGAGAGA GGATGAAGAA AGTAATAAAG ATTGTGTTGG TGGTAAAAGA 
GGAAGAGCCC AAACGGCTCC AACAAAAACA TCCCCTAGAA ATGCAAAAAA GCATGATGAG 
TTATGGCATG ATGGAGTATG CCCATCAGTA TCCAATCCTT TAGAAGTTTA CCTCATCTCC 
TCGCCACCTG AGAATATAAC TTTTGAAGAC CCGTCCTTAG ATGTGATTCT TCTTCTAAGA 
GTTTTACATG CCATCAGCCG ATACTGGTAT TACTTGTATG ATAATGCAAT GTGCAAGGAG 
ATTATTCCAA CTAGTGAATT TATTAACAGT AAGTTGACAG CAAAAGCAAA TAGGCAGCTT 
CAAGATCCTT TAGTAATCAT GACAGGAAAT ATCCCAACGT GGCTTACTGA ACTAGGAAAA 
ACCTGCCCAT TTTTCTTCCC TTTTGATACC CGGCAAATGC TTTTTTACGT AACTGCATTT 
GATCGGGACC GAGCAATGCA AAGATTACTT GATACCAACC CAGAAATCAA CCAGTCGGAT 
TCTCAAGATA GCAGAGTTGC ACCTAGATTG GATAGAAAAA AACGTACTGT GAACAGAGAG 
GAGCTGCTGA AACAAGCGGA GTCTGTAATG CAGGATCTCG GTAGCTCACG GGCCATGTTA 
GAAATCCAGT ATGAAAATGA GGTTGGTACA GGTCTTGGGC CTACACTGGA GTTTTATGCA 
CTTGTATCTC AGGAACTTCA GAGAGCTGAC TTGGGTCTCT GGAGAGGTGA AGAAGTGACT 
CTCAGCAATC CAAAAGGAAG CCAAGAAGGG ACCAAGTATA TTCAAAACCT CCAGGGCCTG 
TTTGCACTTC CCTTTGGTAG GACAGCTAAG CCAGCCCATA TCGCAAAGGT TAAAATGAAG 
TTTCGCTTCT TAGGAAAATT AATGGCCAAG GCTATCATGG ATTTCAGATT GGTGGACCTT 
CCCCTTGGTT TACCCTTTTA TAAGTGGATG CTAAGGCAAG AAACTTCACT GACGTCGCAT 
GATTTGTTTG ACATTGACCC AGTTGTAGCC AGATCAGTGT ATCACCTAGA AGACATTGTC 
AGACAGAAGA AAAGACTTGA GCAAGATAAA TCCCAGACCA AAGAGAGTCT ACAATATGCA 
TTAGAAACCC TGACTATGAA TGGCTGCTCA GTTGAAGATC TAGGATTGGA TTTCACGCTG 
CCAGGGTTTC CCAACATTGA ACTGAAGAAA GGAGGAAAGG ATATACCAGT TACCATCCAC 
AATTTAGAGG AGTATCTAAG ACTGGTTATA TTCTGGGCAC TAAATGAAGG CGTTTCCAGG 
CAATTTGATT CCTTCAGAGA TGGATTTGAA TCTGTCTTCC CACTTAGTCA TCTTCAGTAC 
TTCTACCCGG AAGAACTGGA TCAGCTCTTG TGCGGCAGTA AAGCAGACAC GTGGGATGCG 
AAGACACTGA TGGAGTGCTG CAGGCCGGAC CACGGTTATA CTCATGACAG TCGGGCTGTG 
AAGTTCTTGT TTGAGATTCT CAGTAGTTTT GATAATGAGC AGCAGAGGTT GTTTCTCCAG 
TTTGTGACAG GTAGCCCAAG ATTGCCTGTT GGAGGCTTCC GGAGTCTGAA TCCACCTCTG 
ACAATTGTCC GGAAGACCTT CGAGTCCACG GAGAACCCCG ACGACTTCTT GCCCTCTGTA 
ATGACTTGTG TGAACTACCT TAAGTTGCCG GACTACTCGA GCATTGACAT CATGCGTGAG 
AAACTGTTGA TTGCAGCGAG AGAAGGGCAG CAGTCATTCC ATCTTTCCTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005635864.1
CDS	231..6341
Translation	MSHLGQAKHKGYSPPESRKSNSKAPKVQSNTTSELSRGHLSKRSCSSSSAVIVPQPEDPDRANTSERQKTGQVPKKDNSRGVKRSASPDYNRTNSPSSAKKPKALQHTESPSETNKPHSKSKKRHLDQEQQLKSAQSPSTSKAHTRKSGATGGSRSQKRKRTESSCLKSGSVSESTGAEERSAKPTKLASKSAASAKAGCSTITDSSSAASTSSSSSAVASASSTVPPGARVKQGKDQSKARRSRSASSPSPRRSSREKEQSKTGGSSKFDWAARFSPKVSLPKTKLSLPGSSKSETSKPGPSGLQAKLASLRKSTKKRSESPPAELPSLRRSTRQKTTGSCASASRRGSGLGKRGAAEARRQEKMADPESSQETVNSSAARTDETPQGAAASSSVAGAVGMTTSGESESDDSEMGRLQALLEARGLPPHLFGPLGPRMSQLFHRTIGSGASSKAQQLLQGLQASDESQQLQAVIEMCQLLVMGNEETLGGFPVKSVVPALITLLQMEHNFDIMNHACRALTYMMEALPRSSAVVVDAIPVFLEKLQVIQCIDVAEQALTALEMLSRRHSKAILQAGGLADCLLYLEFFSINAQRNALAIAANCCQSITPDEFHFVADSLPLLTQRLTHQDKKSVESTCLCFARLVDNFQHEENLLQQVASKDLLTNVQQLLVVTPPILSSGMFIMVVRMFSLMCSNCPTLAVQLMKQNIAETLHFLLCGASNGSCQEQIDLVPRSPQELYELTSLICELMPCLPKEGIFAVDTMLKKGNAQNTDGAIWQWRDDRGLWHPYNRIDSRIIEVAAHQVGEDEISLSTLGRVYTIDFNSMQQINEDTGTARAIQRKPNPLANTNTSGYSELKKDDARAQLMKEDPELAKSFIKTLFGVLYEVYSSSAGPAVRHKCLRAILRIIYFADAELLKDVLKNHAVSSHIASMLSSQDLKIVVGALQMAEILMQKLPDIFSVYFRREGVMHQVKHLAESESLLTSPPKACTNGSGSLGSTTSVSSGTATAATNAAADLGSPSLQHSRDDSLDLSPQGRLSDVLKRKRLPKRGPRRPKYSPPRDDDKVDNQAKSPTTTQSPKSSFLASLNPKTWGRLSAQSNSNNIEPARTAGVSGLARAASKDTISNNREKIKGWIKEQAHKFVERYFSSENMDGSNPALNVLQRLCAATEQLNLQVDGGAECLVEIRSIVSESDVSSFEIQHSGFVKQLLLYLTSKSEKDAVSREIRLKRFLHVFFSSPLPGEEPIGRVEPVGNAPLLALVHKMNNCLSQMEQFPVKVHDFPSGNGTGGSFSLNRGSQALKFFNTHQLKCQLQRHPDCANVKQWKGGPVKIDPLALVQAIERYLVVRGYGRVREDDEDSDDDGSDEEIDESLAAQFLNSGNVRHRLQFYIGEHLLPYNMTVYQAVRQFSIQAEDERESTDDESNPLGRAGIWTKTHTIWYKPVREDEESNKDCVGGKRGRAQTAPTKTSPRNAKKHDELWHDGVCPSVSNPLEVYLISSPPENITFEDPSLDVILLLRVLHAISRYWYYLYDNAMCKEIIPTSEFINSKLTAKANRQLQDPLVIMTGNIPTWLTELGKTCPFFFPFDTRQMLFYVTAFDRDRAMQRLLDTNPEINQSDSQDSRVAPRLDRKKRTVNREELLKQAESVMQDLGSSRAMLEIQYENEVGTGLGPTLEFYALVSQELQRADLGLWRGEEVTLSNPKGSQEGTKYIQNLQGLFALPFGRTAKPAHIAKVKMKFRFLGKLMAKAIMDFRLVDLPLGLPFYKWMLRQETSLTSHDLFDIDPVVARSVYHLEDIVRQKKRLEQDKSQTKESLQYALETLTMNGCSVEDLGLDFTLPGFPNIELKKGGKDIPVTIHNLEEYLRLVIFWALNEGVSRQFDSFRDGFESVFPLSHLQYFYPEELDQLLCGSKADTWDAKTLMECCRPDHGYTHDSRAVKFLFEILSSFDNEQQRLFLQFVTGSPRLPVGGFRSLNPPLTIVRKTFESTENPDDFLPSVMTCVNYLKLPDYSSIDIMREKLLIAAREGQQSFHLS

Target ORF information:

RefSeq Version	XM_005635807.1
Organism	Canis lupus familiaris(Dog)
Definition	Canis lupus familiaris thyroid hormone receptor interactor 12 (TRIP12), transcript variant X7, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005635807.1

ORF Insert Sequence:

ATGTCACATT TAGGGCAGGC AAAACATAAG GGATATAGCC CTCCTGAGAG TAGAAAATCT 
AATTCTAAGG CACCCAAAGT GCAGTCTAAT ACTACTTCTG AACTGTCAAG GGGACACCTT 
TCTAAAAGAA GCTGCAGTTC ATCATCTGCT GTCATAGTTC CACAACCAGA GGATCCAGAC 
AGAGCCAATA CTTCAGAAAG GCAAAAAACG GGGCAGGTGC CTAAGAAAGA CAATTCTCGA 
GGAGTGAAAC GCAGTGCTAG TCCAGACTAC AACAGGACCA ATTCTCCTAG CTCTGCCAAA 
AAACCAAAAG CACTTCAGCA TACTGAATCT CCCTCAGAAA CAAATAAGCC ACATAGTAAG 
TCGAAGAAGA GACATTTAGA CCAGGAGCAA CAACTGAAAT CTGCACAGTC GCCATCAACA 
AGTAAGGCTC ACACCAGAAA GAGTGGGGCC ACCGGCGGCT CACGGAGTCA GAAAAGAAAG 
AGGACAGAGA GTTCTTGTCT AAAGAGTGGT TCTGTGTCTG AGTCAACTGG TGCCGAAGAG 
AGATCTGCAA AACCCACCAA GCTGGCTTCA AAATCCGCCG CCTCGGCCAA AGCTGGGTGC 
AGCACCATCA CTGATTCTTC CTCCGCTGCC TCTACTTCCT CCTCATCTTC TGCTGTCGCC 
TCGGCCTCCT CCACTGTACC ACCAGGGGCC AGGGTGAAAC AAGGAAAAGA CCAGAGCAAG 
GCCAGGCGTT CCCGTTCAGC ATCCAGTCCC AGTCCCAGAA GAAGTAGCAG GGAAAAGGAA 
CAGAGTAAAA CTGGTGGCTC TTCAAAATTC GATTGGGCTG CTCGTTTCAG CCCTAAAGTT 
AGCCTTCCTA AAACCAAACT GTCTCTTCCA GGGTCTTCTA AGTCAGAGAC ATCAAAACCT 
GGACCTTCTG GATTACAGGC CAAATTAGCA AGTTTAAGAA AATCTACCAA GAAGCGCAGT 
GAATCCCCAC CTGCTGAGCT CCCCAGTTTG AGGCGGAGCA CACGCCAAAA GACCACGGGC 
TCCTGTGCTA GTGCCAGTCG GCGAGGCTCT GGCCTGGGCA AAAGAGGAGC AGCTGAAGCT 
CGTCGACAGG AGAAGATGGC GGACCCTGAA AGCAGCCAGG AGACCGTGAA TTCTTCAGCT 
GCACGGACAG ACGAAACTCC CCAAGGAGCT GCAGCCTCTA GTTCTGTTGC AGGGGCTGTG 
GGCATGACCA CCTCTGGGGA GAGTGAATCC GATGATTCTG AGATGGGACG ACTACAAGCT 
TTGTTAGAGG CCAGGGGTCT TCCTCCTCAT CTGTTTGGCC CTCTCGGTCC TCGGATGTCA 
CAACTTTTCC ATAGAACAAT TGGAAGCGGA GCTAGCTCTA AGGCCCAGCA GCTTCTGCAA 
GGACTACAAG CAAGTGATGA AAGCCAGCAA CTCCAGGCAG TTATTGAAAT GTGTCAGTTA 
TTGGTTATGG GAAATGAGGA GACGCTGGGA GGGTTTCCGG TCAAGAGTGT CGTTCCAGCT 
TTGATAACAT TACTGCAGAT GGAGCATAAT TTCGATATTA TGAACCATGC TTGTCGAGCC 
TTAACATATA TGATGGAAGC ACTTCCTCGG TCATCTGCTG TTGTAGTAGA TGCAATTCCT 
GTCTTTTTAG AAAAGCTGCA AGTTATTCAG TGTATTGATG TCGCAGAGCA GGCCCTGACT 
GCCTTGGAGA TGTTATCACG CAGACACAGT AAAGCCATTC TACAGGCGGG TGGTTTGGCA 
GACTGCTTGC TGTATCTAGA ATTCTTCAGC ATAAATGCCC AAAGAAATGC ACTAGCAATT 
GCAGCCAATT GCTGCCAGAG TATCACGCCA GATGAATTTC ATTTTGTGGC AGATTCCCTC 
CCGTTGCTTA CCCAAAGGCT AACCCATCAG GACAAAAAGT CAGTTGAAAG CACTTGCCTT 
TGTTTTGCAC GTCTAGTGGA CAACTTCCAA CATGAGGAGA ATTTACTCCA GCAGGTTGCC 
TCCAAAGATC TGCTTACGAA TGTTCAACAA CTCTTGGTAG TGACTCCACC CATTTTAAGT 
TCTGGGATGT TTATAATGGT GGTTCGCATG TTTTCTCTGA TGTGTTCCAA CTGTCCAACT 
TTAGCTGTTC AACTTATGAA GCAAAACATT GCAGAAACAC TTCACTTTCT CCTGTGTGGT 
GCCTCTAATG GAAGTTGTCA GGAACAGATT GATCTTGTTC CACGAAGTCC ACAAGAACTG 
TATGAACTGA CATCTCTGAT TTGCGAACTT ATGCCATGTT TACCAAAAGA AGGCATTTTT 
GCAGTTGATA CCATGCTGAA GAAGGGAAAT GCGCAGAACA CAGACGGTGC GATTTGGCAG 
TGGCGTGATG ACCGTGGCCT TTGGCATCCC TATAACAGGA TTGACAGCCG GATCATTGAG 
GTAGCAGCCC ATCAGGTCGG TGAGGATGAG ATAAGCTTGT CCACTCTGGG ACGAGTTTAT 
ACTATTGATT TTAATTCTAT GCAGCAAATC AATGAGGACA CGGGAACAGC ACGTGCCATT 
CAGAGAAAAC CTAACCCCTT AGCCAATACT AACACTAGTG GATATTCAGA GTTAAAGAAG 
GATGATGCTC GAGCACAGCT TATGAAAGAG GATCCGGAAC TGGCTAAGTC TTTTATTAAG 
ACATTATTTG GTGTTCTTTA TGAAGTGTAT AGTTCCTCAG CAGGACCTGC GGTCAGACAT 
AAGTGCCTTA GAGCAATTCT TAGGATAATT TATTTTGCGG ATGCTGAACT TCTGAAGGAT 
GTCCTGAAAA ATCATGCTGT TTCAAGTCAC ATTGCTTCCA TGCTGTCAAG CCAAGACCTG 
AAGATAGTAG TTGGAGCACT TCAGATGGCA GAAATCTTAA TGCAGAAGTT ACCTGATATT 
TTTAGTGTTT ACTTCAGAAG AGAAGGTGTA ATGCATCAAG TAAAACACTT AGCAGAATCA 
GAGTCTTTGT TGACAAGTCC ACCAAAGGCA TGTACGAATG GGTCAGGATC CCTGGGATCC 
ACGACGTCAG TCAGCAGTGG AACAGCCACG GCCGCCACCA ATGCTGCAGC TGACCTGGGG 
TCCCCCAGCT TGCAGCACAG CAGAGATGAC TCTTTAGATC TGAGCCCTCA AGGTCGATTA 
AGTGATGTTC TAAAGAGAAA ACGGCTGCCA AAACGAGGGC CAAGAAGGCC AAAGTATTCA 
CCTCCAAGAG ATGATGACAA AGTAGACAAT CAAGCTAAAA GCCCTACCAC TACTCAGTCA 
CCTAAATCTT CTTTCCTGGC AAGCTTGAAT CCAAAAACGT GGGGAAGGTT AAGCGCACAG 
TCCAACAGCA ACAACATCGA ACCAGCACGA ACTGCGGGAG TCAGTGGTCT TGCCAGGGCC 
GCCTCCAAGG ACACCATATC CAATAACAGA GAAAAAATTA AAGGTTGGAT TAAGGAACAG 
GCGCATAAAT TTGTAGAGCG TTATTTCAGT TCTGAGAACA TGGATGGAAG CAACCCTGCA 
CTGAATGTCC TTCAGAGACT TTGTGCTGCA ACTGAACAAC TCAACCTCCA GGTGGATGGT 
GGAGCTGAGT GCCTTGTAGA AATCCGTAGC ATAGTCTCAG AGTCTGATGT TTCATCATTT 
GAAATCCAAC ATAGTGGATT TGTGAAGCAG CTGTTACTTT ATTTGACATC TAAAAGTGAA 
AAGGATGCTG TGAGCCGAGA GATCAGATTA AAGAGATTCC TTCACGTATT TTTTTCTTCA 
CCACTTCCTG GAGAAGAGCC CATTGGAAGA GTAGAACCAG TGGGTAATGC GCCTTTGTTG 
GCATTAGTTC ACAAGATGAA CAACTGCCTC AGCCAGATGG AACAGTTTCC AGTCAAAGTA 
CATGACTTCC CCAGTGGGAA CGGGACAGGA GGCAGCTTTT CTCTCAACAG AGGATCACAG 
GCTTTAAAAT TTTTCAACAC ACACCAGTTA AAATGCCAGT TACAAAGACA TCCAGACTGT 
GCAAATGTGA AGCAGTGGAA GGGCGGGCCG GTCAAGATTG ATCCCCTGGC TTTGGTACAA 
GCTATTGAGA GATACCTTGT AGTTAGAGGG TATGGAAGAG TAAGAGAAGA TGATGAAGAC 
AGTGATGATG ATGGCTCTGA TGAGGAAATA GACGAGTCTC TGGCCGCTCA GTTCTTAAAT 
TCCGGAAACG TAAGGCACAG GCTACAGTTT TATATTGGAG AACATTTGCT ACCATATAAC 
ATGACTGTAT ATCAGGCAGT CCGGCAGTTC AGTATACAAG CTGAGGATGA GCGGGAGTCC 
ACAGATGATG AGAGCAACCC TCTGGGAAGA GCTGGTATTT GGACAAAGAC TCATACAATA 
TGGTACAAAC CTGTGAGAGA GGATGAAGAA AGTAATAAAG ATTGTGTTGG TGGTAAAAGA 
GGAAGAGCCC AAACGGCTCC AACAAAAACA TCCCCTAGAA ATGCAAAAAA GCATGATGAG 
TTATGGCATG ATGGAGTATG CCCATCAGTA TCCAATCCTT TAGAAGTTTA CCTCATCTCC 
TCGCCACCTG AGAATATAAC TTTTGAAGAC CCGTCCTTAG ATGTGATTCT TCTTCTAAGA 
GTTTTACATG CCATCAGCCG ATACTGGTAT TACTTGTATG ATAATGCAAT GTGCAAGGAG 
ATTATTCCAA CTAGTGAATT TATTAACAGT AAGTTGACAG CAAAAGCAAA TAGGCAGCTT 
CAAGATCCTT TAGTAATCAT GACAGGAAAT ATCCCAACGT GGCTTACTGA ACTAGGAAAA 
ACCTGCCCAT TTTTCTTCCC TTTTGATACC CGGCAAATGC TTTTTTACGT AACTGCATTT 
GATCGGGACC GAGCAATGCA AAGATTACTT GATACCAACC CAGAAATCAA CCAGTCGGAT 
TCTCAAGATA GCAGAGTTGC ACCTAGATTG GATAGAAAAA AACGTACTGT GAACAGAGAG 
GAGCTGCTGA AACAAGCGGA GTCTGTAATG CAGGATCTCG GTAGCTCACG GGCCATGTTA 
GAAATCCAGT ATGAAAATGA GGTTGGTACA GGTCTTGGGC CTACACTGGA GTTTTATGCA 
CTTGTATCTC AGGAACTTCA GAGAGCTGAC TTGGGTCTCT GGAGAGGTGA AGAAGTGACT 
CTCAGCAATC CAAAAGGAAG CCAAGAAGGG ACCAAGTATA TTCAAAACCT CCAGGGCCTG 
TTTGCACTTC CCTTTGGTAG GACAGCTAAG CCAGCCCATA TCGCAAAGGT TAAAATGAAG 
TTTCGCTTCT TAGGAAAATT AATGGCCAAG GCTATCATGG ATTTCAGATT GGTGGACCTT 
CCCCTTGGTT TACCCTTTTA TAAGTGGATG CTAAGGCAAG AAACTTCACT GACGTCGCAT 
GATTTGTTTG ACATTGACCC AGTTGTAGCC AGATCAGTGT ATCACCTAGA AGACATTGTC 
AGACAGAAGA AAAGACTTGA GCAAGATAAA TCCCAGACCA AAGAGAGTCT ACAATATGCA 
TTAGAAACCC TGACTATGAA TGGCTGCTCA GTTGAAGATC TAGGATTGGA TTTCACGCTG 
CCAGGGTTTC CCAACATTGA ACTGAAGAAA GGAGGAAAGG ATATACCAGT TACCATCCAC 
AATTTAGAGG AGTATCTAAG ACTGGTTATA TTCTGGGCAC TAAATGAAGG CGTTTCCAGG 
CAATTTGATT CCTTCAGAGA TGGATTTGAA TCTGTCTTCC CACTTAGTCA TCTTCAGTAC 
TTCTACCCGG AAGAACTGGA TCAGCTCTTG TGCGGCAGTA AAGCAGACAC GTGGGATGCG 
AAGACACTGA TGGAGTGCTG CAGGCCGGAC CACGGTTATA CTCATGACAG TCGGGCTGTG 
AAGTTCTTGT TTGAGATTCT CAGTAGTTTT GATAATGAGC AGCAGAGGTT GTTTCTCCAG 
TTTGTGACAG GTAGCCCAAG ATTGCCTGTT GGAGGCTTCC GGAGTCTGAA TCCACCTCTG 
ACAATTGTCC GGAAGACCTT CGAGTCCACG GAGAACCCCG ACGACTTCTT GCCCTCTGTA 
ATGACTTGTG TGAACTACCT TAAGTTGCCG GACTACTCGA GCATTGACAT CATGCGTGAG 
AAACTGTTGA TTGCAGCGAG AGAAGGGCAG CAGTCATTCC ATCTTTCCTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TRIP12 ( XM_005635808.3 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_005635865.1 Canis lupus familiaris thyroid hormone receptor interactor 12 (TRIP12), transcript variant X12, mRNA.

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CloneID	OCe25768
Clone ID Related Accession (Same CDS sequence)	XM_005635808.3 , XM_005635808.2
Accession Version	XM_005635808.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6081bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-09-04
Organism	Canis lupus familiaris(dog)
Product	E3 ubiquitin-protein ligase TRIP12 isoform X10
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006607.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 5, 2017 this sequence version replaced XM_005635808.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCCAACC GGCCTAATAA CAATCCAGGG GGGTCACTGC GACGTTCACA GAGGAACACT 
GCCGGGGCCC AACCACAAGA CGACTCGATA GGAGGAAGAA GCTGCAGTTC ATCATCTGCT 
GTCATAGTTC CACAACCAGA GGATCCAGAC AGAGCCAATA CTTCAGAAAG GCAAAAAACG 
GGGCAGGTGC CTAAGAAAGA CAATTCTCGA GGAGTGAAAC GCAGTGCTAG TCCAGACTAC 
AACAGGACCA ATTCTCCTAG CTCTGCCAAA AAACCAAAAG CACTTCAGCA TACTGAATCT 
CCCTCAGAAA CAAATAAGCC ACATAGTAAG TCGAAGAAGA GACATTTAGA CCAGGAGCAA 
CAACTGAAAT CTGCACAGTC GCCATCAACA AGTAAGGCTC ACACCAGAAA GAGTGGGGCC 
ACCGGCGGCT CACGGAGTCA GAAAAGAAAG AGGACAGAGA GTTCTTGTCT AAAGAGTGGT 
TCTGTGTCTG AGTCAACTGG TGCCGAAGAG AGATCTGCAA AACCCACCAA GCTGGCTTCA 
AAATCCGCCG CCTCGGCCAA AGCTGGGTGC AGCACCATCA CTGATTCTTC CTCCGCTGCC 
TCTACTTCCT CCTCATCTTC TGCTGTCGCC TCGGCCTCCT CCACTGTACC ACCAGGGGCC 
AGGGTGAAAC AAGGAAAAGA CCAGAGCAAG GCCAGGCGTT CCCGTTCAGC ATCCAGTCCC 
AGTCCCAGAA GAAGTAGCAG GGAAAAGGAA CAGAGTAAAA CTGGTGGCTC TTCAAAATTC 
GATTGGGCTG CTCGTTTCAG CCCTAAAGTT AGCCTTCCTA AAACCAAACT GTCTCTTCCA 
GGGTCTTCTA AGTCAGAGAC ATCAAAACCT GGACCTTCTG GATTACAGGC CAAATTAGCA 
AGTTTAAGAA AATCTACCAA GAAGCGCAGT GAATCCCCAC CTGCTGAGCT CCCCAGTTTG 
AGGCGGAGCA CACGCCAAAA GACCACGGGC TCCTGTGCTA GTGCCAGTCG GCGAGGCTCT 
GGCCTGGGCA AAAGAGGAGC AGCTGAAGCT CGTCGACAGG AGAAGATGGC GGACCCTGAA 
AGCAGCCAGG AGACCGTGAA TTCTTCAGCT GCACGGACAG ACGAAACTCC CCAAGGAGCT 
GCAGCCTCTA GTTCTGTTGC AGGGGCTGTG GGCATGACCA CCTCTGGGGA GAGTGAATCC 
GATGATTCTG AGATGGGACG ACTACAAGCT TTGTTAGAGG CCAGGGGTCT TCCTCCTCAT 
CTGTTTGGCC CTCTCGGTCC TCGGATGTCA CAACTTTTCC ATAGAACAAT TGGAAGCGGA 
GCTAGCTCTA AGGCCCAGCA GCTTCTGCAA GGACTACAAG CAAGTGATGA AAGCCAGCAA 
CTCCAGGCAG TTATTGAAAT GTGTCAGTTA TTGGTTATGG GAAATGAGGA GACGCTGGGA 
GGGTTTCCGG TCAAGAGTGT CGTTCCAGCT TTGATAACAT TACTGCAGAT GGAGCATAAT 
TTCGATATTA TGAACCATGC TTGTCGAGCC TTAACATATA TGATGGAAGC ACTTCCTCGG 
TCATCTGCTG TTGTAGTAGA TGCAATTCCT GTCTTTTTAG AAAAGCTGCA AGTTATTCAG 
TGTATTGATG TCGCAGAGCA GGCCCTGACT GCCTTGGAGA TGTTATCACG CAGACACAGT 
AAAGCCATTC TACAGGCGGG TGGTTTGGCA GACTGCTTGC TGTATCTAGA ATTCTTCAGC 
ATAAATGCCC AAAGAAATGC ACTAGCAATT GCAGCCAATT GCTGCCAGAG TATCACGCCA 
GATGAATTTC ATTTTGTGGC AGATTCCCTC CCGTTGCTTA CCCAAAGGCT AACCCATCAG 
GACAAAAAGT CAGTTGAAAG CACTTGCCTT TGTTTTGCAC GTCTAGTGGA CAACTTCCAA 
CATGAGGAGA ATTTACTCCA GCAGGTTGCC TCCAAAGATC TGCTTACGAA TGTTCAACAA 
CTCTTGGTAG TGACTCCACC CATTTTAAGT TCTGGGATGT TTATAATGGT GGTTCGCATG 
TTTTCTCTGA TGTGTTCCAA CTGTCCAACT TTAGCTGTTC AACTTATGAA GCAAAACATT 
GCAGAAACAC TTCACTTTCT CCTGTGTGGT GCCTCTAATG GAAGTTGTCA GGAACAGATT 
GATCTTGTTC CACGAAGTCC ACAAGAACTG TATGAACTGA CATCTCTGAT TTGCGAACTT 
ATGCCATGTT TACCAAAAGA AGGCATTTTT GCAGTTGATA CCATGCTGAA GAAGGGAAAT 
GCGCAGAACA CAGACGGTGC GATTTGGCAG TGGCGTGATG ACCGTGGCCT TTGGCATCCC 
TATAACAGGA TTGACAGCCG GATCATTGAG GTAGCAGCCC ATCAGGTCGG TGAGGATGAG 
ATAAGCTTGT CCACTCTGGG ACGAGTTTAT ACTATTGATT TTAATTCTAT GCAGCAAATC 
AATGAGGACA CGGGAACAGC ACGTGCCATT CAGAGAAAAC CTAACCCCTT AGCCAATACT 
AACACTAGTG GATATTCAGA GTTAAAGAAG GATGATGCTC GAGCACAGCT TATGAAAGAG 
GATCCGGAAC TGGCTAAGTC TTTTATTAAG ACATTATTTG GTGTTCTTTA TGAAGTGTAT 
AGTTCCTCAG CAGGACCTGC GGTCAGACAT AAGTGCCTTA GAGCAATTCT TAGGATAATT 
TATTTTGCGG ATGCTGAACT TCTGAAGGAT GTCCTGAAAA ATCATGCTGT TTCAAGTCAC 
ATTGCTTCCA TGCTGTCAAG CCAAGACCTG AAGATAGTAG TTGGAGCACT TCAGATGGCA 
GAAATCTTAA TGCAGAAGTT ACCTGATATT TTTAGTGTTT ACTTCAGAAG AGAAGGTGTA 
ATGCATCAAG TAAAACACTT AGCAGAATCA GAGTCTTTGT TGACAAGTCC ACCAAAGGCA 
TGTACGAATG GGTCAGGATC CCTGGGATCC ACGACGTCAG TCAGCAGTGG AACAGCCACG 
GCCGCCACCA ATGCTGCAGC TGACCTGGGG TCCCCCAGCT TGCAGCACAG CAGAGATGAC 
TCTTTAGATC TGAGCCCTCA AGGTCGATTA AGTGATGTTC TAAAGAGAAA ACGGCTGCCA 
AAACGAGGGC CAAGAAGGCC AAAGTATTCA CCTCCAAGAG ATGATGACAA AGTAGACAAT 
CAAGCTAAAA GCCCTACCAC TACTCAGTCA CCTAAATCTT CTTTCCTGGC AAGCTTGAAT 
CCAAAAACGT GGGGAAGGTT AAGCGCACAG TCCAACAGCA ACAACATCGA ACCAGCACGA 
ACTGCGGGAG TCAGTGGTCT TGCCAGGGCC GCCTCCAAGG ACACCATATC CAATAACAGA 
GAAAAAATTA AAGGTTGGAT TAAGGAACAG GCGCATAAAT TTGTAGAGCG TTATTTCAGT 
TCTGAGAACA TGGATGGAAG CAACCCTGCA CTGAATGTCC TTCAGAGACT TTGTGCTGCA 
ACTGAACAAC TCAACCTCCA GGTGGATGGT GGAGCTGAGT GCCTTGTAGA AATCCGTAGC 
ATAGTCTCAG AGTCTGATGT TTCATCATTT GAAATCCAAC ATAGTGGATT TGTGAAGCAG 
CTGTTACTTT ATTTGACATC TAAAAGTGAA AAGGATGCTG TGAGCCGAGA GATCAGATTA 
AAGAGATTCC TTCACGTATT TTTTTCTTCA CCACTTCCTG GAGAAGAGCC CATTGGAAGA 
GTAGAACCAG TGGGTAATGC GCCTTTGTTG GCATTAGTTC ACAAGATGAA CAACTGCCTC 
AGCCAGATGG AACAGTTTCC AGTCAAAGTA CATGACTTCC CCAGTGGGAA CGGGACAGGA 
GGCAGCTTTT CTCTCAACAG AGGATCACAG GCTTTAAAAT TTTTCAACAC ACACCAGTTA 
AAATGCCAGT TACAAAGACA TCCAGACTGT GCAAATGTGA AGCAGTGGAA GGGCGGGCCG 
GTCAAGATTG ATCCCCTGGC TTTGGTACAA GCTATTGAGA GATACCTTGT AGTTAGAGGG 
TATGGAAGAG TAAGAGAAGA TGATGAAGAC AGTGATGATG ATGGCTCTGA TGAGGAAATA 
GACGAGTCTC TGGCCGCTCA GTTCTTAAAT TCCGGAAACG TAAGGCACAG GCTACAGTTT 
TATATTGGAG AACATTTGCT ACCATATAAC ATGACTGTAT ATCAGGCAGT CCGGCAGTTC 
AGTATACAAG CTGAGGATGA GCGGGAGTCC ACAGATGATG AGAGCAACCC TCTGGGAAGA 
GCTGGTATTT GGACAAAGAC TCATACAATA TGGTACAAAC CTGTGAGAGA GGATGAAGAA 
AGTAATAAAG ATTGTGTTGG TGGTAAAAGA GGAAGAGCCC AAACGGCTCC AACAAAAACA 
TCCCCTAGAA ATGCAAAAAA GCATGATGAG TTATGGCATG ATGGAGTATG CCCATCAGTA 
TCCAATCCTT TAGAAGTTTA CCTCATCTCC TCGCCACCTG AGAATATAAC TTTTGAAGAC 
CCGTCCTTAG ATGTGATTCT TCTTCTAAGA GTTTTACATG CCATCAGCCG ATACTGGTAT 
TACTTGTATG ATAATGCAAT GTGCAAGGAG ATTATTCCAA CTAGTGAATT TATTAACAGT 
AAGTTGACAG CAAAAGCAAA TAGGCAGCTT CAAGATCCTT TAGTAATCAT GACAGGAAAT 
ATCCCAACGT GGCTTACTGA ACTAGGAAAA ACCTGCCCAT TTTTCTTCCC TTTTGATACC 
CGGCAAATGC TTTTTTACGT AACTGCATTT GATCGGGACC GAGCAATGCA AAGATTACTT 
GATACCAACC CAGAAATCAA CCAGTCGGAT TCTCAAGATA GCAGAGTTGC ACCTAGATTG 
GATAGAAAAA AACGTACTGT GAACAGAGAG GAGCTGCTGA AACAAGCGGA GTCTGTAATG 
CAGGATCTCG GTAGCTCACG GGCCATGTTA GAAATCCAGT ATGAAAATGA GGTTGGTACA 
GGTCTTGGGC CTACACTGGA GTTTTATGCA CTTGTATCTC AGGAACTTCA GAGAGCTGAC 
TTGGGTCTCT GGAGAGGTGA AGAAGTGACT CTCAGCAATC CAAAAGGAAG CCAAGAAGGG 
ACCAAGTATA TTCAAAACCT CCAGGGCCTG TTTGCACTTC CCTTTGGTAG GACAGCTAAG 
CCAGCCCATA TCGCAAAGGT TAAAATGAAG TTTCGCTTCT TAGGAAAATT AATGGCCAAG 
GCTATCATGG ATTTCAGATT GGTGGACCTT CCCCTTGGTT TACCCTTTTA TAAGTGGATG 
CTAAGGCAAG AAACTTCACT GACGTCGCAT GATTTGTTTG ACATTGACCC AGTTGTAGCC 
AGATCAGTGT ATCACCTAGA AGACATTGTC AGACAGAAGA AAAGACTTGA GCAAGATAAA 
TCCCAGACCA AAGAGAGTCT ACAATATGCA TTAGAAACCC TGACTATGAA TGGCTGCTCA 
GTTGAAGATC TAGGATTGGA TTTCACGCTG CCAGGGTTTC CCAACATTGA ACTGAAGAAA 
GGAGGAAAGG ATATACCAGT TACCATCCAC AATTTAGAGG AGTATCTAAG ACTGGTTATA 
TTCTGGGCAC TAAATGAAGG CGTTTCCAGG CAATTTGATT CCTTCAGAGA TGGATTTGAA 
TCTGTCTTCC CACTTAGTCA TCTTCAGTAC TTCTACCCGG AAGAACTGGA TCAGCTCTTG 
TGCGGCAGTA AAGCAGACAC GTGGGATGCG AAGACACTGA TGGAGTGCTG CAGGCCGGAC 
CACGGTTATA CTCATGACAG TCGGGCTGTG AAGTTCTTGT TTGAGATTCT CAGTAGTTTT 
GATAATGAGC AGCAGAGGTT GTTTCTCCAG TTTGTGACAG GTAGCCCAAG ATTGCCTGTT 
GGAGGCTTCC GGAGTCTGAA TCCACCTCTG ACAATTGTCC GGAAGACCTT CGAGTCCACG 
GAGAACCCCG ACGACTTCTT GCCCTCTGTA ATGACTTGTG TGAACTACCT TAAGTTGCCG 
GACTACTCGA GCATTGACAT CATGCGTGAG AAACTGTTGA TTGCAGCGAG AGAAGGGCAG 
CAGTCATTCC ATCTTTCCTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005635865.1
CDS	160..6240
Translation	MSNRPNNNPGGSLRRSQRNTAGAQPQDDSIGGRSCSSSSAVIVPQPEDPDRANTSERQKTGQVPKKDNSRGVKRSASPDYNRTNSPSSAKKPKALQHTESPSETNKPHSKSKKRHLDQEQQLKSAQSPSTSKAHTRKSGATGGSRSQKRKRTESSCLKSGSVSESTGAEERSAKPTKLASKSAASAKAGCSTITDSSSAASTSSSSSAVASASSTVPPGARVKQGKDQSKARRSRSASSPSPRRSSREKEQSKTGGSSKFDWAARFSPKVSLPKTKLSLPGSSKSETSKPGPSGLQAKLASLRKSTKKRSESPPAELPSLRRSTRQKTTGSCASASRRGSGLGKRGAAEARRQEKMADPESSQETVNSSAARTDETPQGAAASSSVAGAVGMTTSGESESDDSEMGRLQALLEARGLPPHLFGPLGPRMSQLFHRTIGSGASSKAQQLLQGLQASDESQQLQAVIEMCQLLVMGNEETLGGFPVKSVVPALITLLQMEHNFDIMNHACRALTYMMEALPRSSAVVVDAIPVFLEKLQVIQCIDVAEQALTALEMLSRRHSKAILQAGGLADCLLYLEFFSINAQRNALAIAANCCQSITPDEFHFVADSLPLLTQRLTHQDKKSVESTCLCFARLVDNFQHEENLLQQVASKDLLTNVQQLLVVTPPILSSGMFIMVVRMFSLMCSNCPTLAVQLMKQNIAETLHFLLCGASNGSCQEQIDLVPRSPQELYELTSLICELMPCLPKEGIFAVDTMLKKGNAQNTDGAIWQWRDDRGLWHPYNRIDSRIIEVAAHQVGEDEISLSTLGRVYTIDFNSMQQINEDTGTARAIQRKPNPLANTNTSGYSELKKDDARAQLMKEDPELAKSFIKTLFGVLYEVYSSSAGPAVRHKCLRAILRIIYFADAELLKDVLKNHAVSSHIASMLSSQDLKIVVGALQMAEILMQKLPDIFSVYFRREGVMHQVKHLAESESLLTSPPKACTNGSGSLGSTTSVSSGTATAATNAAADLGSPSLQHSRDDSLDLSPQGRLSDVLKRKRLPKRGPRRPKYSPPRDDDKVDNQAKSPTTTQSPKSSFLASLNPKTWGRLSAQSNSNNIEPARTAGVSGLARAASKDTISNNREKIKGWIKEQAHKFVERYFSSENMDGSNPALNVLQRLCAATEQLNLQVDGGAECLVEIRSIVSESDVSSFEIQHSGFVKQLLLYLTSKSEKDAVSREIRLKRFLHVFFSSPLPGEEPIGRVEPVGNAPLLALVHKMNNCLSQMEQFPVKVHDFPSGNGTGGSFSLNRGSQALKFFNTHQLKCQLQRHPDCANVKQWKGGPVKIDPLALVQAIERYLVVRGYGRVREDDEDSDDDGSDEEIDESLAAQFLNSGNVRHRLQFYIGEHLLPYNMTVYQAVRQFSIQAEDERESTDDESNPLGRAGIWTKTHTIWYKPVREDEESNKDCVGGKRGRAQTAPTKTSPRNAKKHDELWHDGVCPSVSNPLEVYLISSPPENITFEDPSLDVILLLRVLHAISRYWYYLYDNAMCKEIIPTSEFINSKLTAKANRQLQDPLVIMTGNIPTWLTELGKTCPFFFPFDTRQMLFYVTAFDRDRAMQRLLDTNPEINQSDSQDSRVAPRLDRKKRTVNREELLKQAESVMQDLGSSRAMLEIQYENEVGTGLGPTLEFYALVSQELQRADLGLWRGEEVTLSNPKGSQEGTKYIQNLQGLFALPFGRTAKPAHIAKVKMKFRFLGKLMAKAIMDFRLVDLPLGLPFYKWMLRQETSLTSHDLFDIDPVVARSVYHLEDIVRQKKRLEQDKSQTKESLQYALETLTMNGCSVEDLGLDFTLPGFPNIELKKGGKDIPVTIHNLEEYLRLVIFWALNEGVSRQFDSFRDGFESVFPLSHLQYFYPEELDQLLCGSKADTWDAKTLMECCRPDHGYTHDSRAVKFLFEILSSFDNEQQRLFLQFVTGSPRLPVGGFRSLNPPLTIVRKTFESTENPDDFLPSVMTCVNYLKLPDYSSIDIMREKLLIAAREGQQSFHLS

Target ORF information:

RefSeq Version	XM_005635808.3
Organism	Canis lupus familiaris(dog)
Definition	Canis lupus familiaris thyroid hormone receptor interactor 12 (TRIP12), transcript variant X12, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005635808.3

ORF Insert Sequence:

ATGTCCAACC GGCCTAATAA CAATCCAGGG GGGTCACTGC GACGTTCACA GAGGAACACT 
GCCGGGGCCC AACCACAAGA CGACTCGATA GGAGGAAGAA GCTGCAGTTC ATCATCTGCT 
GTCATAGTTC CACAACCAGA GGATCCAGAC AGAGCCAATA CTTCAGAAAG GCAAAAAACG 
GGGCAGGTGC CTAAGAAAGA CAATTCTCGA GGAGTGAAAC GCAGTGCTAG TCCAGACTAC 
AACAGGACCA ATTCTCCTAG CTCTGCCAAA AAACCAAAAG CACTTCAGCA TACTGAATCT 
CCCTCAGAAA CAAATAAGCC ACATAGTAAG TCGAAGAAGA GACATTTAGA CCAGGAGCAA 
CAACTGAAAT CTGCACAGTC GCCATCAACA AGTAAGGCTC ACACCAGAAA GAGTGGGGCC 
ACCGGCGGCT CACGGAGTCA GAAAAGAAAG AGGACAGAGA GTTCTTGTCT AAAGAGTGGT 
TCTGTGTCTG AGTCAACTGG TGCCGAAGAG AGATCTGCAA AACCCACCAA GCTGGCTTCA 
AAATCCGCCG CCTCGGCCAA AGCTGGGTGC AGCACCATCA CTGATTCTTC CTCCGCTGCC 
TCTACTTCCT CCTCATCTTC TGCTGTCGCC TCGGCCTCCT CCACTGTACC ACCAGGGGCC 
AGGGTGAAAC AAGGAAAAGA CCAGAGCAAG GCCAGGCGTT CCCGTTCAGC ATCCAGTCCC 
AGTCCCAGAA GAAGTAGCAG GGAAAAGGAA CAGAGTAAAA CTGGTGGCTC TTCAAAATTC 
GATTGGGCTG CTCGTTTCAG CCCTAAAGTT AGCCTTCCTA AAACCAAACT GTCTCTTCCA 
GGGTCTTCTA AGTCAGAGAC ATCAAAACCT GGACCTTCTG GATTACAGGC CAAATTAGCA 
AGTTTAAGAA AATCTACCAA GAAGCGCAGT GAATCCCCAC CTGCTGAGCT CCCCAGTTTG 
AGGCGGAGCA CACGCCAAAA GACCACGGGC TCCTGTGCTA GTGCCAGTCG GCGAGGCTCT 
GGCCTGGGCA AAAGAGGAGC AGCTGAAGCT CGTCGACAGG AGAAGATGGC GGACCCTGAA 
AGCAGCCAGG AGACCGTGAA TTCTTCAGCT GCACGGACAG ACGAAACTCC CCAAGGAGCT 
GCAGCCTCTA GTTCTGTTGC AGGGGCTGTG GGCATGACCA CCTCTGGGGA GAGTGAATCC 
GATGATTCTG AGATGGGACG ACTACAAGCT TTGTTAGAGG CCAGGGGTCT TCCTCCTCAT 
CTGTTTGGCC CTCTCGGTCC TCGGATGTCA CAACTTTTCC ATAGAACAAT TGGAAGCGGA 
GCTAGCTCTA AGGCCCAGCA GCTTCTGCAA GGACTACAAG CAAGTGATGA AAGCCAGCAA 
CTCCAGGCAG TTATTGAAAT GTGTCAGTTA TTGGTTATGG GAAATGAGGA GACGCTGGGA 
GGGTTTCCGG TCAAGAGTGT CGTTCCAGCT TTGATAACAT TACTGCAGAT GGAGCATAAT 
TTCGATATTA TGAACCATGC TTGTCGAGCC TTAACATATA TGATGGAAGC ACTTCCTCGG 
TCATCTGCTG TTGTAGTAGA TGCAATTCCT GTCTTTTTAG AAAAGCTGCA AGTTATTCAG 
TGTATTGATG TCGCAGAGCA GGCCCTGACT GCCTTGGAGA TGTTATCACG CAGACACAGT 
AAAGCCATTC TACAGGCGGG TGGTTTGGCA GACTGCTTGC TGTATCTAGA ATTCTTCAGC 
ATAAATGCCC AAAGAAATGC ACTAGCAATT GCAGCCAATT GCTGCCAGAG TATCACGCCA 
GATGAATTTC ATTTTGTGGC AGATTCCCTC CCGTTGCTTA CCCAAAGGCT AACCCATCAG 
GACAAAAAGT CAGTTGAAAG CACTTGCCTT TGTTTTGCAC GTCTAGTGGA CAACTTCCAA 
CATGAGGAGA ATTTACTCCA GCAGGTTGCC TCCAAAGATC TGCTTACGAA TGTTCAACAA 
CTCTTGGTAG TGACTCCACC CATTTTAAGT TCTGGGATGT TTATAATGGT GGTTCGCATG 
TTTTCTCTGA TGTGTTCCAA CTGTCCAACT TTAGCTGTTC AACTTATGAA GCAAAACATT 
GCAGAAACAC TTCACTTTCT CCTGTGTGGT GCCTCTAATG GAAGTTGTCA GGAACAGATT 
GATCTTGTTC CACGAAGTCC ACAAGAACTG TATGAACTGA CATCTCTGAT TTGCGAACTT 
ATGCCATGTT TACCAAAAGA AGGCATTTTT GCAGTTGATA CCATGCTGAA GAAGGGAAAT 
GCGCAGAACA CAGACGGTGC GATTTGGCAG TGGCGTGATG ACCGTGGCCT TTGGCATCCC 
TATAACAGGA TTGACAGCCG GATCATTGAG GTAGCAGCCC ATCAGGTCGG TGAGGATGAG 
ATAAGCTTGT CCACTCTGGG ACGAGTTTAT ACTATTGATT TTAATTCTAT GCAGCAAATC 
AATGAGGACA CGGGAACAGC ACGTGCCATT CAGAGAAAAC CTAACCCCTT AGCCAATACT 
AACACTAGTG GATATTCAGA GTTAAAGAAG GATGATGCTC GAGCACAGCT TATGAAAGAG 
GATCCGGAAC TGGCTAAGTC TTTTATTAAG ACATTATTTG GTGTTCTTTA TGAAGTGTAT 
AGTTCCTCAG CAGGACCTGC GGTCAGACAT AAGTGCCTTA GAGCAATTCT TAGGATAATT 
TATTTTGCGG ATGCTGAACT TCTGAAGGAT GTCCTGAAAA ATCATGCTGT TTCAAGTCAC 
ATTGCTTCCA TGCTGTCAAG CCAAGACCTG AAGATAGTAG TTGGAGCACT TCAGATGGCA 
GAAATCTTAA TGCAGAAGTT ACCTGATATT TTTAGTGTTT ACTTCAGAAG AGAAGGTGTA 
ATGCATCAAG TAAAACACTT AGCAGAATCA GAGTCTTTGT TGACAAGTCC ACCAAAGGCA 
TGTACGAATG GGTCAGGATC CCTGGGATCC ACGACGTCAG TCAGCAGTGG AACAGCCACG 
GCCGCCACCA ATGCTGCAGC TGACCTGGGG TCCCCCAGCT TGCAGCACAG CAGAGATGAC 
TCTTTAGATC TGAGCCCTCA AGGTCGATTA AGTGATGTTC TAAAGAGAAA ACGGCTGCCA 
AAACGAGGGC CAAGAAGGCC AAAGTATTCA CCTCCAAGAG ATGATGACAA AGTAGACAAT 
CAAGCTAAAA GCCCTACCAC TACTCAGTCA CCTAAATCTT CTTTCCTGGC AAGCTTGAAT 
CCAAAAACGT GGGGAAGGTT AAGCGCACAG TCCAACAGCA ACAACATCGA ACCAGCACGA 
ACTGCGGGAG TCAGTGGTCT TGCCAGGGCC GCCTCCAAGG ACACCATATC CAATAACAGA 
GAAAAAATTA AAGGTTGGAT TAAGGAACAG GCGCATAAAT TTGTAGAGCG TTATTTCAGT 
TCTGAGAACA TGGATGGAAG CAACCCTGCA CTGAATGTCC TTCAGAGACT TTGTGCTGCA 
ACTGAACAAC TCAACCTCCA GGTGGATGGT GGAGCTGAGT GCCTTGTAGA AATCCGTAGC 
ATAGTCTCAG AGTCTGATGT TTCATCATTT GAAATCCAAC ATAGTGGATT TGTGAAGCAG 
CTGTTACTTT ATTTGACATC TAAAAGTGAA AAGGATGCTG TGAGCCGAGA GATCAGATTA 
AAGAGATTCC TTCACGTATT TTTTTCTTCA CCACTTCCTG GAGAAGAGCC CATTGGAAGA 
GTAGAACCAG TGGGTAATGC GCCTTTGTTG GCATTAGTTC ACAAGATGAA CAACTGCCTC 
AGCCAGATGG AACAGTTTCC AGTCAAAGTA CATGACTTCC CCAGTGGGAA CGGGACAGGA 
GGCAGCTTTT CTCTCAACAG AGGATCACAG GCTTTAAAAT TTTTCAACAC ACACCAGTTA 
AAATGCCAGT TACAAAGACA TCCAGACTGT GCAAATGTGA AGCAGTGGAA GGGCGGGCCG 
GTCAAGATTG ATCCCCTGGC TTTGGTACAA GCTATTGAGA GATACCTTGT AGTTAGAGGG 
TATGGAAGAG TAAGAGAAGA TGATGAAGAC AGTGATGATG ATGGCTCTGA TGAGGAAATA 
GACGAGTCTC TGGCCGCTCA GTTCTTAAAT TCCGGAAACG TAAGGCACAG GCTACAGTTT 
TATATTGGAG AACATTTGCT ACCATATAAC ATGACTGTAT ATCAGGCAGT CCGGCAGTTC 
AGTATACAAG CTGAGGATGA GCGGGAGTCC ACAGATGATG AGAGCAACCC TCTGGGAAGA 
GCTGGTATTT GGACAAAGAC TCATACAATA TGGTACAAAC CTGTGAGAGA GGATGAAGAA 
AGTAATAAAG ATTGTGTTGG TGGTAAAAGA GGAAGAGCCC AAACGGCTCC AACAAAAACA 
TCCCCTAGAA ATGCAAAAAA GCATGATGAG TTATGGCATG ATGGAGTATG CCCATCAGTA 
TCCAATCCTT TAGAAGTTTA CCTCATCTCC TCGCCACCTG AGAATATAAC TTTTGAAGAC 
CCGTCCTTAG ATGTGATTCT TCTTCTAAGA GTTTTACATG CCATCAGCCG ATACTGGTAT 
TACTTGTATG ATAATGCAAT GTGCAAGGAG ATTATTCCAA CTAGTGAATT TATTAACAGT 
AAGTTGACAG CAAAAGCAAA TAGGCAGCTT CAAGATCCTT TAGTAATCAT GACAGGAAAT 
ATCCCAACGT GGCTTACTGA ACTAGGAAAA ACCTGCCCAT TTTTCTTCCC TTTTGATACC 
CGGCAAATGC TTTTTTACGT AACTGCATTT GATCGGGACC GAGCAATGCA AAGATTACTT 
GATACCAACC CAGAAATCAA CCAGTCGGAT TCTCAAGATA GCAGAGTTGC ACCTAGATTG 
GATAGAAAAA AACGTACTGT GAACAGAGAG GAGCTGCTGA AACAAGCGGA GTCTGTAATG 
CAGGATCTCG GTAGCTCACG GGCCATGTTA GAAATCCAGT ATGAAAATGA GGTTGGTACA 
GGTCTTGGGC CTACACTGGA GTTTTATGCA CTTGTATCTC AGGAACTTCA GAGAGCTGAC 
TTGGGTCTCT GGAGAGGTGA AGAAGTGACT CTCAGCAATC CAAAAGGAAG CCAAGAAGGG 
ACCAAGTATA TTCAAAACCT CCAGGGCCTG TTTGCACTTC CCTTTGGTAG GACAGCTAAG 
CCAGCCCATA TCGCAAAGGT TAAAATGAAG TTTCGCTTCT TAGGAAAATT AATGGCCAAG 
GCTATCATGG ATTTCAGATT GGTGGACCTT CCCCTTGGTT TACCCTTTTA TAAGTGGATG 
CTAAGGCAAG AAACTTCACT GACGTCGCAT GATTTGTTTG ACATTGACCC AGTTGTAGCC 
AGATCAGTGT ATCACCTAGA AGACATTGTC AGACAGAAGA AAAGACTTGA GCAAGATAAA 
TCCCAGACCA AAGAGAGTCT ACAATATGCA TTAGAAACCC TGACTATGAA TGGCTGCTCA 
GTTGAAGATC TAGGATTGGA TTTCACGCTG CCAGGGTTTC CCAACATTGA ACTGAAGAAA 
GGAGGAAAGG ATATACCAGT TACCATCCAC AATTTAGAGG AGTATCTAAG ACTGGTTATA 
TTCTGGGCAC TAAATGAAGG CGTTTCCAGG CAATTTGATT CCTTCAGAGA TGGATTTGAA 
TCTGTCTTCC CACTTAGTCA TCTTCAGTAC TTCTACCCGG AAGAACTGGA TCAGCTCTTG 
TGCGGCAGTA AAGCAGACAC GTGGGATGCG AAGACACTGA TGGAGTGCTG CAGGCCGGAC 
CACGGTTATA CTCATGACAG TCGGGCTGTG AAGTTCTTGT TTGAGATTCT CAGTAGTTTT 
GATAATGAGC AGCAGAGGTT GTTTCTCCAG TTTGTGACAG GTAGCCCAAG ATTGCCTGTT 
GGAGGCTTCC GGAGTCTGAA TCCACCTCTG ACAATTGTCC GGAAGACCTT CGAGTCCACG 
GAGAACCCCG ACGACTTCTT GCCCTCTGTA ATGACTTGTG TGAACTACCT TAAGTTGCCG 
GACTACTCGA GCATTGACAT CATGCGTGAG AAACTGTTGA TTGCAGCGAG AGAAGGGCAG 
CAGTCATTCC ATCTTTCCTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TRIP12 ( XM_005635808.2 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_005635865.1 Canis lupus familiaris thyroid hormone receptor interactor 12 (TRIP12), transcript variant X8, mRNA.

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CloneID	OCe25768
Clone ID Related Accession (Same CDS sequence)	XM_005635808.3 , XM_005635808.2
Accession Version	XM_005635808.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6081bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-09-16
Organism	Canis lupus familiaris(Dog)
Product	E3 ubiquitin-protein ligase TRIP12 isoform X7
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726103.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 17, 2015 this sequence version replaced XM_005635808.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCCAACC GGCCTAATAA CAATCCAGGG GGGTCACTGC GACGTTCACA GAGGAACACT 
GCCGGGGCCC AACCACAAGA CGACTCGATA GGAGGAAGAA GCTGCAGTTC ATCATCTGCT 
GTCATAGTTC CACAACCAGA GGATCCAGAC AGAGCCAATA CTTCAGAAAG GCAAAAAACG 
GGGCAGGTGC CTAAGAAAGA CAATTCTCGA GGAGTGAAAC GCAGTGCTAG TCCAGACTAC 
AACAGGACCA ATTCTCCTAG CTCTGCCAAA AAACCAAAAG CACTTCAGCA TACTGAATCT 
CCCTCAGAAA CAAATAAGCC ACATAGTAAG TCGAAGAAGA GACATTTAGA CCAGGAGCAA 
CAACTGAAAT CTGCACAGTC GCCATCAACA AGTAAGGCTC ACACCAGAAA GAGTGGGGCC 
ACCGGCGGCT CACGGAGTCA GAAAAGAAAG AGGACAGAGA GTTCTTGTCT AAAGAGTGGT 
TCTGTGTCTG AGTCAACTGG TGCCGAAGAG AGATCTGCAA AACCCACCAA GCTGGCTTCA 
AAATCCGCCG CCTCGGCCAA AGCTGGGTGC AGCACCATCA CTGATTCTTC CTCCGCTGCC 
TCTACTTCCT CCTCATCTTC TGCTGTCGCC TCGGCCTCCT CCACTGTACC ACCAGGGGCC 
AGGGTGAAAC AAGGAAAAGA CCAGAGCAAG GCCAGGCGTT CCCGTTCAGC ATCCAGTCCC 
AGTCCCAGAA GAAGTAGCAG GGAAAAGGAA CAGAGTAAAA CTGGTGGCTC TTCAAAATTC 
GATTGGGCTG CTCGTTTCAG CCCTAAAGTT AGCCTTCCTA AAACCAAACT GTCTCTTCCA 
GGGTCTTCTA AGTCAGAGAC ATCAAAACCT GGACCTTCTG GATTACAGGC CAAATTAGCA 
AGTTTAAGAA AATCTACCAA GAAGCGCAGT GAATCCCCAC CTGCTGAGCT CCCCAGTTTG 
AGGCGGAGCA CACGCCAAAA GACCACGGGC TCCTGTGCTA GTGCCAGTCG GCGAGGCTCT 
GGCCTGGGCA AAAGAGGAGC AGCTGAAGCT CGTCGACAGG AGAAGATGGC GGACCCTGAA 
AGCAGCCAGG AGACCGTGAA TTCTTCAGCT GCACGGACAG ACGAAACTCC CCAAGGAGCT 
GCAGCCTCTA GTTCTGTTGC AGGGGCTGTG GGCATGACCA CCTCTGGGGA GAGTGAATCC 
GATGATTCTG AGATGGGACG ACTACAAGCT TTGTTAGAGG CCAGGGGTCT TCCTCCTCAT 
CTGTTTGGCC CTCTCGGTCC TCGGATGTCA CAACTTTTCC ATAGAACAAT TGGAAGCGGA 
GCTAGCTCTA AGGCCCAGCA GCTTCTGCAA GGACTACAAG CAAGTGATGA AAGCCAGCAA 
CTCCAGGCAG TTATTGAAAT GTGTCAGTTA TTGGTTATGG GAAATGAGGA GACGCTGGGA 
GGGTTTCCGG TCAAGAGTGT CGTTCCAGCT TTGATAACAT TACTGCAGAT GGAGCATAAT 
TTCGATATTA TGAACCATGC TTGTCGAGCC TTAACATATA TGATGGAAGC ACTTCCTCGG 
TCATCTGCTG TTGTAGTAGA TGCAATTCCT GTCTTTTTAG AAAAGCTGCA AGTTATTCAG 
TGTATTGATG TCGCAGAGCA GGCCCTGACT GCCTTGGAGA TGTTATCACG CAGACACAGT 
AAAGCCATTC TACAGGCGGG TGGTTTGGCA GACTGCTTGC TGTATCTAGA ATTCTTCAGC 
ATAAATGCCC AAAGAAATGC ACTAGCAATT GCAGCCAATT GCTGCCAGAG TATCACGCCA 
GATGAATTTC ATTTTGTGGC AGATTCCCTC CCGTTGCTTA CCCAAAGGCT AACCCATCAG 
GACAAAAAGT CAGTTGAAAG CACTTGCCTT TGTTTTGCAC GTCTAGTGGA CAACTTCCAA 
CATGAGGAGA ATTTACTCCA GCAGGTTGCC TCCAAAGATC TGCTTACGAA TGTTCAACAA 
CTCTTGGTAG TGACTCCACC CATTTTAAGT TCTGGGATGT TTATAATGGT GGTTCGCATG 
TTTTCTCTGA TGTGTTCCAA CTGTCCAACT TTAGCTGTTC AACTTATGAA GCAAAACATT 
GCAGAAACAC TTCACTTTCT CCTGTGTGGT GCCTCTAATG GAAGTTGTCA GGAACAGATT 
GATCTTGTTC CACGAAGTCC ACAAGAACTG TATGAACTGA CATCTCTGAT TTGCGAACTT 
ATGCCATGTT TACCAAAAGA AGGCATTTTT GCAGTTGATA CCATGCTGAA GAAGGGAAAT 
GCGCAGAACA CAGACGGTGC GATTTGGCAG TGGCGTGATG ACCGTGGCCT TTGGCATCCC 
TATAACAGGA TTGACAGCCG GATCATTGAG GTAGCAGCCC ATCAGGTCGG TGAGGATGAG 
ATAAGCTTGT CCACTCTGGG ACGAGTTTAT ACTATTGATT TTAATTCTAT GCAGCAAATC 
AATGAGGACA CGGGAACAGC ACGTGCCATT CAGAGAAAAC CTAACCCCTT AGCCAATACT 
AACACTAGTG GATATTCAGA GTTAAAGAAG GATGATGCTC GAGCACAGCT TATGAAAGAG 
GATCCGGAAC TGGCTAAGTC TTTTATTAAG ACATTATTTG GTGTTCTTTA TGAAGTGTAT 
AGTTCCTCAG CAGGACCTGC GGTCAGACAT AAGTGCCTTA GAGCAATTCT TAGGATAATT 
TATTTTGCGG ATGCTGAACT TCTGAAGGAT GTCCTGAAAA ATCATGCTGT TTCAAGTCAC 
ATTGCTTCCA TGCTGTCAAG CCAAGACCTG AAGATAGTAG TTGGAGCACT TCAGATGGCA 
GAAATCTTAA TGCAGAAGTT ACCTGATATT TTTAGTGTTT ACTTCAGAAG AGAAGGTGTA 
ATGCATCAAG TAAAACACTT AGCAGAATCA GAGTCTTTGT TGACAAGTCC ACCAAAGGCA 
TGTACGAATG GGTCAGGATC CCTGGGATCC ACGACGTCAG TCAGCAGTGG AACAGCCACG 
GCCGCCACCA ATGCTGCAGC TGACCTGGGG TCCCCCAGCT TGCAGCACAG CAGAGATGAC 
TCTTTAGATC TGAGCCCTCA AGGTCGATTA AGTGATGTTC TAAAGAGAAA ACGGCTGCCA 
AAACGAGGGC CAAGAAGGCC AAAGTATTCA CCTCCAAGAG ATGATGACAA AGTAGACAAT 
CAAGCTAAAA GCCCTACCAC TACTCAGTCA CCTAAATCTT CTTTCCTGGC AAGCTTGAAT 
CCAAAAACGT GGGGAAGGTT AAGCGCACAG TCCAACAGCA ACAACATCGA ACCAGCACGA 
ACTGCGGGAG TCAGTGGTCT TGCCAGGGCC GCCTCCAAGG ACACCATATC CAATAACAGA 
GAAAAAATTA AAGGTTGGAT TAAGGAACAG GCGCATAAAT TTGTAGAGCG TTATTTCAGT 
TCTGAGAACA TGGATGGAAG CAACCCTGCA CTGAATGTCC TTCAGAGACT TTGTGCTGCA 
ACTGAACAAC TCAACCTCCA GGTGGATGGT GGAGCTGAGT GCCTTGTAGA AATCCGTAGC 
ATAGTCTCAG AGTCTGATGT TTCATCATTT GAAATCCAAC ATAGTGGATT TGTGAAGCAG 
CTGTTACTTT ATTTGACATC TAAAAGTGAA AAGGATGCTG TGAGCCGAGA GATCAGATTA 
AAGAGATTCC TTCACGTATT TTTTTCTTCA CCACTTCCTG GAGAAGAGCC CATTGGAAGA 
GTAGAACCAG TGGGTAATGC GCCTTTGTTG GCATTAGTTC ACAAGATGAA CAACTGCCTC 
AGCCAGATGG AACAGTTTCC AGTCAAAGTA CATGACTTCC CCAGTGGGAA CGGGACAGGA 
GGCAGCTTTT CTCTCAACAG AGGATCACAG GCTTTAAAAT TTTTCAACAC ACACCAGTTA 
AAATGCCAGT TACAAAGACA TCCAGACTGT GCAAATGTGA AGCAGTGGAA GGGCGGGCCG 
GTCAAGATTG ATCCCCTGGC TTTGGTACAA GCTATTGAGA GATACCTTGT AGTTAGAGGG 
TATGGAAGAG TAAGAGAAGA TGATGAAGAC AGTGATGATG ATGGCTCTGA TGAGGAAATA 
GACGAGTCTC TGGCCGCTCA GTTCTTAAAT TCCGGAAACG TAAGGCACAG GCTACAGTTT 
TATATTGGAG AACATTTGCT ACCATATAAC ATGACTGTAT ATCAGGCAGT CCGGCAGTTC 
AGTATACAAG CTGAGGATGA GCGGGAGTCC ACAGATGATG AGAGCAACCC TCTGGGAAGA 
GCTGGTATTT GGACAAAGAC TCATACAATA TGGTACAAAC CTGTGAGAGA GGATGAAGAA 
AGTAATAAAG ATTGTGTTGG TGGTAAAAGA GGAAGAGCCC AAACGGCTCC AACAAAAACA 
TCCCCTAGAA ATGCAAAAAA GCATGATGAG TTATGGCATG ATGGAGTATG CCCATCAGTA 
TCCAATCCTT TAGAAGTTTA CCTCATCTCC TCGCCACCTG AGAATATAAC TTTTGAAGAC 
CCGTCCTTAG ATGTGATTCT TCTTCTAAGA GTTTTACATG CCATCAGCCG ATACTGGTAT 
TACTTGTATG ATAATGCAAT GTGCAAGGAG ATTATTCCAA CTAGTGAATT TATTAACAGT 
AAGTTGACAG CAAAAGCAAA TAGGCAGCTT CAAGATCCTT TAGTAATCAT GACAGGAAAT 
ATCCCAACGT GGCTTACTGA ACTAGGAAAA ACCTGCCCAT TTTTCTTCCC TTTTGATACC 
CGGCAAATGC TTTTTTACGT AACTGCATTT GATCGGGACC GAGCAATGCA AAGATTACTT 
GATACCAACC CAGAAATCAA CCAGTCGGAT TCTCAAGATA GCAGAGTTGC ACCTAGATTG 
GATAGAAAAA AACGTACTGT GAACAGAGAG GAGCTGCTGA AACAAGCGGA GTCTGTAATG 
CAGGATCTCG GTAGCTCACG GGCCATGTTA GAAATCCAGT ATGAAAATGA GGTTGGTACA 
GGTCTTGGGC CTACACTGGA GTTTTATGCA CTTGTATCTC AGGAACTTCA GAGAGCTGAC 
TTGGGTCTCT GGAGAGGTGA AGAAGTGACT CTCAGCAATC CAAAAGGAAG CCAAGAAGGG 
ACCAAGTATA TTCAAAACCT CCAGGGCCTG TTTGCACTTC CCTTTGGTAG GACAGCTAAG 
CCAGCCCATA TCGCAAAGGT TAAAATGAAG TTTCGCTTCT TAGGAAAATT AATGGCCAAG 
GCTATCATGG ATTTCAGATT GGTGGACCTT CCCCTTGGTT TACCCTTTTA TAAGTGGATG 
CTAAGGCAAG AAACTTCACT GACGTCGCAT GATTTGTTTG ACATTGACCC AGTTGTAGCC 
AGATCAGTGT ATCACCTAGA AGACATTGTC AGACAGAAGA AAAGACTTGA GCAAGATAAA 
TCCCAGACCA AAGAGAGTCT ACAATATGCA TTAGAAACCC TGACTATGAA TGGCTGCTCA 
GTTGAAGATC TAGGATTGGA TTTCACGCTG CCAGGGTTTC CCAACATTGA ACTGAAGAAA 
GGAGGAAAGG ATATACCAGT TACCATCCAC AATTTAGAGG AGTATCTAAG ACTGGTTATA 
TTCTGGGCAC TAAATGAAGG CGTTTCCAGG CAATTTGATT CCTTCAGAGA TGGATTTGAA 
TCTGTCTTCC CACTTAGTCA TCTTCAGTAC TTCTACCCGG AAGAACTGGA TCAGCTCTTG 
TGCGGCAGTA AAGCAGACAC GTGGGATGCG AAGACACTGA TGGAGTGCTG CAGGCCGGAC 
CACGGTTATA CTCATGACAG TCGGGCTGTG AAGTTCTTGT TTGAGATTCT CAGTAGTTTT 
GATAATGAGC AGCAGAGGTT GTTTCTCCAG TTTGTGACAG GTAGCCCAAG ATTGCCTGTT 
GGAGGCTTCC GGAGTCTGAA TCCACCTCTG ACAATTGTCC GGAAGACCTT CGAGTCCACG 
GAGAACCCCG ACGACTTCTT GCCCTCTGTA ATGACTTGTG TGAACTACCT TAAGTTGCCG 
GACTACTCGA GCATTGACAT CATGCGTGAG AAACTGTTGA TTGCAGCGAG AGAAGGGCAG 
CAGTCATTCC ATCTTTCCTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005635865.1
CDS	188..6268
Translation	MSNRPNNNPGGSLRRSQRNTAGAQPQDDSIGGRSCSSSSAVIVPQPEDPDRANTSERQKTGQVPKKDNSRGVKRSASPDYNRTNSPSSAKKPKALQHTESPSETNKPHSKSKKRHLDQEQQLKSAQSPSTSKAHTRKSGATGGSRSQKRKRTESSCLKSGSVSESTGAEERSAKPTKLASKSAASAKAGCSTITDSSSAASTSSSSSAVASASSTVPPGARVKQGKDQSKARRSRSASSPSPRRSSREKEQSKTGGSSKFDWAARFSPKVSLPKTKLSLPGSSKSETSKPGPSGLQAKLASLRKSTKKRSESPPAELPSLRRSTRQKTTGSCASASRRGSGLGKRGAAEARRQEKMADPESSQETVNSSAARTDETPQGAAASSSVAGAVGMTTSGESESDDSEMGRLQALLEARGLPPHLFGPLGPRMSQLFHRTIGSGASSKAQQLLQGLQASDESQQLQAVIEMCQLLVMGNEETLGGFPVKSVVPALITLLQMEHNFDIMNHACRALTYMMEALPRSSAVVVDAIPVFLEKLQVIQCIDVAEQALTALEMLSRRHSKAILQAGGLADCLLYLEFFSINAQRNALAIAANCCQSITPDEFHFVADSLPLLTQRLTHQDKKSVESTCLCFARLVDNFQHEENLLQQVASKDLLTNVQQLLVVTPPILSSGMFIMVVRMFSLMCSNCPTLAVQLMKQNIAETLHFLLCGASNGSCQEQIDLVPRSPQELYELTSLICELMPCLPKEGIFAVDTMLKKGNAQNTDGAIWQWRDDRGLWHPYNRIDSRIIEVAAHQVGEDEISLSTLGRVYTIDFNSMQQINEDTGTARAIQRKPNPLANTNTSGYSELKKDDARAQLMKEDPELAKSFIKTLFGVLYEVYSSSAGPAVRHKCLRAILRIIYFADAELLKDVLKNHAVSSHIASMLSSQDLKIVVGALQMAEILMQKLPDIFSVYFRREGVMHQVKHLAESESLLTSPPKACTNGSGSLGSTTSVSSGTATAATNAAADLGSPSLQHSRDDSLDLSPQGRLSDVLKRKRLPKRGPRRPKYSPPRDDDKVDNQAKSPTTTQSPKSSFLASLNPKTWGRLSAQSNSNNIEPARTAGVSGLARAASKDTISNNREKIKGWIKEQAHKFVERYFSSENMDGSNPALNVLQRLCAATEQLNLQVDGGAECLVEIRSIVSESDVSSFEIQHSGFVKQLLLYLTSKSEKDAVSREIRLKRFLHVFFSSPLPGEEPIGRVEPVGNAPLLALVHKMNNCLSQMEQFPVKVHDFPSGNGTGGSFSLNRGSQALKFFNTHQLKCQLQRHPDCANVKQWKGGPVKIDPLALVQAIERYLVVRGYGRVREDDEDSDDDGSDEEIDESLAAQFLNSGNVRHRLQFYIGEHLLPYNMTVYQAVRQFSIQAEDERESTDDESNPLGRAGIWTKTHTIWYKPVREDEESNKDCVGGKRGRAQTAPTKTSPRNAKKHDELWHDGVCPSVSNPLEVYLISSPPENITFEDPSLDVILLLRVLHAISRYWYYLYDNAMCKEIIPTSEFINSKLTAKANRQLQDPLVIMTGNIPTWLTELGKTCPFFFPFDTRQMLFYVTAFDRDRAMQRLLDTNPEINQSDSQDSRVAPRLDRKKRTVNREELLKQAESVMQDLGSSRAMLEIQYENEVGTGLGPTLEFYALVSQELQRADLGLWRGEEVTLSNPKGSQEGTKYIQNLQGLFALPFGRTAKPAHIAKVKMKFRFLGKLMAKAIMDFRLVDLPLGLPFYKWMLRQETSLTSHDLFDIDPVVARSVYHLEDIVRQKKRLEQDKSQTKESLQYALETLTMNGCSVEDLGLDFTLPGFPNIELKKGGKDIPVTIHNLEEYLRLVIFWALNEGVSRQFDSFRDGFESVFPLSHLQYFYPEELDQLLCGSKADTWDAKTLMECCRPDHGYTHDSRAVKFLFEILSSFDNEQQRLFLQFVTGSPRLPVGGFRSLNPPLTIVRKTFESTENPDDFLPSVMTCVNYLKLPDYSSIDIMREKLLIAAREGQQSFHLS

Target ORF information:

RefSeq Version	XM_005635808.2
Organism	Canis lupus familiaris(Dog)
Definition	Canis lupus familiaris thyroid hormone receptor interactor 12 (TRIP12), transcript variant X8, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005635808.2

ORF Insert Sequence:

ATGTCCAACC GGCCTAATAA CAATCCAGGG GGGTCACTGC GACGTTCACA GAGGAACACT 
GCCGGGGCCC AACCACAAGA CGACTCGATA GGAGGAAGAA GCTGCAGTTC ATCATCTGCT 
GTCATAGTTC CACAACCAGA GGATCCAGAC AGAGCCAATA CTTCAGAAAG GCAAAAAACG 
GGGCAGGTGC CTAAGAAAGA CAATTCTCGA GGAGTGAAAC GCAGTGCTAG TCCAGACTAC 
AACAGGACCA ATTCTCCTAG CTCTGCCAAA AAACCAAAAG CACTTCAGCA TACTGAATCT 
CCCTCAGAAA CAAATAAGCC ACATAGTAAG TCGAAGAAGA GACATTTAGA CCAGGAGCAA 
CAACTGAAAT CTGCACAGTC GCCATCAACA AGTAAGGCTC ACACCAGAAA GAGTGGGGCC 
ACCGGCGGCT CACGGAGTCA GAAAAGAAAG AGGACAGAGA GTTCTTGTCT AAAGAGTGGT 
TCTGTGTCTG AGTCAACTGG TGCCGAAGAG AGATCTGCAA AACCCACCAA GCTGGCTTCA 
AAATCCGCCG CCTCGGCCAA AGCTGGGTGC AGCACCATCA CTGATTCTTC CTCCGCTGCC 
TCTACTTCCT CCTCATCTTC TGCTGTCGCC TCGGCCTCCT CCACTGTACC ACCAGGGGCC 
AGGGTGAAAC AAGGAAAAGA CCAGAGCAAG GCCAGGCGTT CCCGTTCAGC ATCCAGTCCC 
AGTCCCAGAA GAAGTAGCAG GGAAAAGGAA CAGAGTAAAA CTGGTGGCTC TTCAAAATTC 
GATTGGGCTG CTCGTTTCAG CCCTAAAGTT AGCCTTCCTA AAACCAAACT GTCTCTTCCA 
GGGTCTTCTA AGTCAGAGAC ATCAAAACCT GGACCTTCTG GATTACAGGC CAAATTAGCA 
AGTTTAAGAA AATCTACCAA GAAGCGCAGT GAATCCCCAC CTGCTGAGCT CCCCAGTTTG 
AGGCGGAGCA CACGCCAAAA GACCACGGGC TCCTGTGCTA GTGCCAGTCG GCGAGGCTCT 
GGCCTGGGCA AAAGAGGAGC AGCTGAAGCT CGTCGACAGG AGAAGATGGC GGACCCTGAA 
AGCAGCCAGG AGACCGTGAA TTCTTCAGCT GCACGGACAG ACGAAACTCC CCAAGGAGCT 
GCAGCCTCTA GTTCTGTTGC AGGGGCTGTG GGCATGACCA CCTCTGGGGA GAGTGAATCC 
GATGATTCTG AGATGGGACG ACTACAAGCT TTGTTAGAGG CCAGGGGTCT TCCTCCTCAT 
CTGTTTGGCC CTCTCGGTCC TCGGATGTCA CAACTTTTCC ATAGAACAAT TGGAAGCGGA 
GCTAGCTCTA AGGCCCAGCA GCTTCTGCAA GGACTACAAG CAAGTGATGA AAGCCAGCAA 
CTCCAGGCAG TTATTGAAAT GTGTCAGTTA TTGGTTATGG GAAATGAGGA GACGCTGGGA 
GGGTTTCCGG TCAAGAGTGT CGTTCCAGCT TTGATAACAT TACTGCAGAT GGAGCATAAT 
TTCGATATTA TGAACCATGC TTGTCGAGCC TTAACATATA TGATGGAAGC ACTTCCTCGG 
TCATCTGCTG TTGTAGTAGA TGCAATTCCT GTCTTTTTAG AAAAGCTGCA AGTTATTCAG 
TGTATTGATG TCGCAGAGCA GGCCCTGACT GCCTTGGAGA TGTTATCACG CAGACACAGT 
AAAGCCATTC TACAGGCGGG TGGTTTGGCA GACTGCTTGC TGTATCTAGA ATTCTTCAGC 
ATAAATGCCC AAAGAAATGC ACTAGCAATT GCAGCCAATT GCTGCCAGAG TATCACGCCA 
GATGAATTTC ATTTTGTGGC AGATTCCCTC CCGTTGCTTA CCCAAAGGCT AACCCATCAG 
GACAAAAAGT CAGTTGAAAG CACTTGCCTT TGTTTTGCAC GTCTAGTGGA CAACTTCCAA 
CATGAGGAGA ATTTACTCCA GCAGGTTGCC TCCAAAGATC TGCTTACGAA TGTTCAACAA 
CTCTTGGTAG TGACTCCACC CATTTTAAGT TCTGGGATGT TTATAATGGT GGTTCGCATG 
TTTTCTCTGA TGTGTTCCAA CTGTCCAACT TTAGCTGTTC AACTTATGAA GCAAAACATT 
GCAGAAACAC TTCACTTTCT CCTGTGTGGT GCCTCTAATG GAAGTTGTCA GGAACAGATT 
GATCTTGTTC CACGAAGTCC ACAAGAACTG TATGAACTGA CATCTCTGAT TTGCGAACTT 
ATGCCATGTT TACCAAAAGA AGGCATTTTT GCAGTTGATA CCATGCTGAA GAAGGGAAAT 
GCGCAGAACA CAGACGGTGC GATTTGGCAG TGGCGTGATG ACCGTGGCCT TTGGCATCCC 
TATAACAGGA TTGACAGCCG GATCATTGAG GTAGCAGCCC ATCAGGTCGG TGAGGATGAG 
ATAAGCTTGT CCACTCTGGG ACGAGTTTAT ACTATTGATT TTAATTCTAT GCAGCAAATC 
AATGAGGACA CGGGAACAGC ACGTGCCATT CAGAGAAAAC CTAACCCCTT AGCCAATACT 
AACACTAGTG GATATTCAGA GTTAAAGAAG GATGATGCTC GAGCACAGCT TATGAAAGAG 
GATCCGGAAC TGGCTAAGTC TTTTATTAAG ACATTATTTG GTGTTCTTTA TGAAGTGTAT 
AGTTCCTCAG CAGGACCTGC GGTCAGACAT AAGTGCCTTA GAGCAATTCT TAGGATAATT 
TATTTTGCGG ATGCTGAACT TCTGAAGGAT GTCCTGAAAA ATCATGCTGT TTCAAGTCAC 
ATTGCTTCCA TGCTGTCAAG CCAAGACCTG AAGATAGTAG TTGGAGCACT TCAGATGGCA 
GAAATCTTAA TGCAGAAGTT ACCTGATATT TTTAGTGTTT ACTTCAGAAG AGAAGGTGTA 
ATGCATCAAG TAAAACACTT AGCAGAATCA GAGTCTTTGT TGACAAGTCC ACCAAAGGCA 
TGTACGAATG GGTCAGGATC CCTGGGATCC ACGACGTCAG TCAGCAGTGG AACAGCCACG 
GCCGCCACCA ATGCTGCAGC TGACCTGGGG TCCCCCAGCT TGCAGCACAG CAGAGATGAC 
TCTTTAGATC TGAGCCCTCA AGGTCGATTA AGTGATGTTC TAAAGAGAAA ACGGCTGCCA 
AAACGAGGGC CAAGAAGGCC AAAGTATTCA CCTCCAAGAG ATGATGACAA AGTAGACAAT 
CAAGCTAAAA GCCCTACCAC TACTCAGTCA CCTAAATCTT CTTTCCTGGC AAGCTTGAAT 
CCAAAAACGT GGGGAAGGTT AAGCGCACAG TCCAACAGCA ACAACATCGA ACCAGCACGA 
ACTGCGGGAG TCAGTGGTCT TGCCAGGGCC GCCTCCAAGG ACACCATATC CAATAACAGA 
GAAAAAATTA AAGGTTGGAT TAAGGAACAG GCGCATAAAT TTGTAGAGCG TTATTTCAGT 
TCTGAGAACA TGGATGGAAG CAACCCTGCA CTGAATGTCC TTCAGAGACT TTGTGCTGCA 
ACTGAACAAC TCAACCTCCA GGTGGATGGT GGAGCTGAGT GCCTTGTAGA AATCCGTAGC 
ATAGTCTCAG AGTCTGATGT TTCATCATTT GAAATCCAAC ATAGTGGATT TGTGAAGCAG 
CTGTTACTTT ATTTGACATC TAAAAGTGAA AAGGATGCTG TGAGCCGAGA GATCAGATTA 
AAGAGATTCC TTCACGTATT TTTTTCTTCA CCACTTCCTG GAGAAGAGCC CATTGGAAGA 
GTAGAACCAG TGGGTAATGC GCCTTTGTTG GCATTAGTTC ACAAGATGAA CAACTGCCTC 
AGCCAGATGG AACAGTTTCC AGTCAAAGTA CATGACTTCC CCAGTGGGAA CGGGACAGGA 
GGCAGCTTTT CTCTCAACAG AGGATCACAG GCTTTAAAAT TTTTCAACAC ACACCAGTTA 
AAATGCCAGT TACAAAGACA TCCAGACTGT GCAAATGTGA AGCAGTGGAA GGGCGGGCCG 
GTCAAGATTG ATCCCCTGGC TTTGGTACAA GCTATTGAGA GATACCTTGT AGTTAGAGGG 
TATGGAAGAG TAAGAGAAGA TGATGAAGAC AGTGATGATG ATGGCTCTGA TGAGGAAATA 
GACGAGTCTC TGGCCGCTCA GTTCTTAAAT TCCGGAAACG TAAGGCACAG GCTACAGTTT 
TATATTGGAG AACATTTGCT ACCATATAAC ATGACTGTAT ATCAGGCAGT CCGGCAGTTC 
AGTATACAAG CTGAGGATGA GCGGGAGTCC ACAGATGATG AGAGCAACCC TCTGGGAAGA 
GCTGGTATTT GGACAAAGAC TCATACAATA TGGTACAAAC CTGTGAGAGA GGATGAAGAA 
AGTAATAAAG ATTGTGTTGG TGGTAAAAGA GGAAGAGCCC AAACGGCTCC AACAAAAACA 
TCCCCTAGAA ATGCAAAAAA GCATGATGAG TTATGGCATG ATGGAGTATG CCCATCAGTA 
TCCAATCCTT TAGAAGTTTA CCTCATCTCC TCGCCACCTG AGAATATAAC TTTTGAAGAC 
CCGTCCTTAG ATGTGATTCT TCTTCTAAGA GTTTTACATG CCATCAGCCG ATACTGGTAT 
TACTTGTATG ATAATGCAAT GTGCAAGGAG ATTATTCCAA CTAGTGAATT TATTAACAGT 
AAGTTGACAG CAAAAGCAAA TAGGCAGCTT CAAGATCCTT TAGTAATCAT GACAGGAAAT 
ATCCCAACGT GGCTTACTGA ACTAGGAAAA ACCTGCCCAT TTTTCTTCCC TTTTGATACC 
CGGCAAATGC TTTTTTACGT AACTGCATTT GATCGGGACC GAGCAATGCA AAGATTACTT 
GATACCAACC CAGAAATCAA CCAGTCGGAT TCTCAAGATA GCAGAGTTGC ACCTAGATTG 
GATAGAAAAA AACGTACTGT GAACAGAGAG GAGCTGCTGA AACAAGCGGA GTCTGTAATG 
CAGGATCTCG GTAGCTCACG GGCCATGTTA GAAATCCAGT ATGAAAATGA GGTTGGTACA 
GGTCTTGGGC CTACACTGGA GTTTTATGCA CTTGTATCTC AGGAACTTCA GAGAGCTGAC 
TTGGGTCTCT GGAGAGGTGA AGAAGTGACT CTCAGCAATC CAAAAGGAAG CCAAGAAGGG 
ACCAAGTATA TTCAAAACCT CCAGGGCCTG TTTGCACTTC CCTTTGGTAG GACAGCTAAG 
CCAGCCCATA TCGCAAAGGT TAAAATGAAG TTTCGCTTCT TAGGAAAATT AATGGCCAAG 
GCTATCATGG ATTTCAGATT GGTGGACCTT CCCCTTGGTT TACCCTTTTA TAAGTGGATG 
CTAAGGCAAG AAACTTCACT GACGTCGCAT GATTTGTTTG ACATTGACCC AGTTGTAGCC 
AGATCAGTGT ATCACCTAGA AGACATTGTC AGACAGAAGA AAAGACTTGA GCAAGATAAA 
TCCCAGACCA AAGAGAGTCT ACAATATGCA TTAGAAACCC TGACTATGAA TGGCTGCTCA 
GTTGAAGATC TAGGATTGGA TTTCACGCTG CCAGGGTTTC CCAACATTGA ACTGAAGAAA 
GGAGGAAAGG ATATACCAGT TACCATCCAC AATTTAGAGG AGTATCTAAG ACTGGTTATA 
TTCTGGGCAC TAAATGAAGG CGTTTCCAGG CAATTTGATT CCTTCAGAGA TGGATTTGAA 
TCTGTCTTCC CACTTAGTCA TCTTCAGTAC TTCTACCCGG AAGAACTGGA TCAGCTCTTG 
TGCGGCAGTA AAGCAGACAC GTGGGATGCG AAGACACTGA TGGAGTGCTG CAGGCCGGAC 
CACGGTTATA CTCATGACAG TCGGGCTGTG AAGTTCTTGT TTGAGATTCT CAGTAGTTTT 
GATAATGAGC AGCAGAGGTT GTTTCTCCAG TTTGTGACAG GTAGCCCAAG ATTGCCTGTT 
GGAGGCTTCC GGAGTCTGAA TCCACCTCTG ACAATTGTCC GGAAGACCTT CGAGTCCACG 
GAGAACCCCG ACGACTTCTT GCCCTCTGTA ATGACTTGTG TGAACTACCT TAAGTTGCCG 
GACTACTCGA GCATTGACAT CATGCGTGAG AAACTGTTGA TTGCAGCGAG AGAAGGGCAG 
CAGTCATTCC ATCTTTCCTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TRIP12 ( XM_022409672.1 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_022265380.1 Canis lupus familiaris thyroid hormone receptor interactor 12 (TRIP12), transcript variant X13, mRNA.

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CloneID	OCe25769
Clone ID Related Accession (Same CDS sequence)	XM_022409672.1 , XM_005635809.2
Accession Version	XM_022409672.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6078bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-09-04
Organism	Canis lupus familiaris(dog)
Product	E3 ubiquitin-protein ligase TRIP12 isoform X11
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006607.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCCAACC GGCCTAATAA CAATCCAGGG GGGTCACTGC GACGTTCACA GAGGAACACT 
GCCGGGGCCC AACCACAAGA CGACTCGATA GGAGGAAGAA GCTGCAGTTC ATCATCTGCT 
GTCATAGTTC CACAACCAGA GGATCCAGAC AGAGCCAATA CTTCAGAAAG GCAAAAAACG 
GGGCAGGTGC CTAAGAAAGA CAATTCTCGA GGAGTGAAAC GCAGTGCTAG TCCAGACTAC 
AACAGGACCA ATTCTCCTAG CTCTGCCAAA AAACCAAAAG CACTTCAGCA TACTGAATCT 
CCCTCAGAAA CAAATAAGCC ACATAGTAAG TCGAAGAAGA GACATTTAGA CCAGGAGCAA 
CAACTGAAAT CTGCACAGTC GCCATCAACA AGTAAGGCTC ACACCAGAAA GAGTGGGGCC 
ACCGGCGGCT CACGGAGTCA GAAAAGAAAG AGGACAGAGA GTTCTTGTCT AAAGAGTGGT 
TCTGTGTCTG AGTCAACTGG TGCCGAAGAG AGATCTGCAA AACCCACCAA GCTGGCTTCA 
AAATCCGCCG CCTCGGCCAA AGCTGGGTGC AGCACCATCA CTGATTCTTC CTCCGCTGCC 
TCTACTTCCT CCTCATCTTC TGCTGTCGCC TCGGCCTCCT CCACTGTACC ACCAGGGGCC 
AGGGTGAAAC AAGGAAAAGA CCAGAGCAAG GCCAGGCGTT CCCGTTCAGC ATCCAGTCCC 
AGTCCCAGAA GAAGTAGCAG GGAAAAGGAA CAGAGTAAAA CTGGTGGCTC TTCAAAATTC 
GATTGGGCTG CTCGTTTCAG CCCTAAAGTT AGCCTTCCTA AAACCAAACT GTCTCTTCCA 
GGGTCTTCTA AGTCAGAGAC ATCAAAACCT GGACCTTCTG GATTACAGGC CAAATTAGCA 
AGTTTAAGAA AATCTACCAA GAAGCGCAGT GAATCCCCAC CTGCTGAGCT CCCCAGTTTG 
AGGCGGAGCA CACGCCAAAA GACCACGGGC TCCTGTGCTA GTGCCAGTCG GCGAGGCTCT 
GGCCTGGGCA AAAGAGGAGC AGCTGAAGCT CGTCGACAGG AGAAGATGGC GGACCCTGAA 
AGCAGCCAGG AGACCGTGAA TTCTTCAGCT GCACGGACAG ACGAAACTCC CCAAGGAGCT 
GCAGCCTCTA GTTCTGTTGC AGGGGCTGTG GGCATGACCA CCTCTGGGGA GAGTGAATCC 
GATGATTCTG AGATGGGACG ACTACAAGCT TTGTTAGAGG CCAGGGGTCT TCCTCCTCAT 
CTGTTTGGCC CTCTCGGTCC TCGGATGTCA CAACTTTTCC ATAGAACAAT TGGAAGCGGA 
GCTAGCTCTA AGGCCCAGCA GCTTCTGCAA GGACTACAAG CAAGTGATGA AAGCCAGCAA 
CTCCAGGCAG TTATTGAAAT GTGTCAGTTA TTGGTTATGG GAAATGAGGA GACGCTGGGA 
GGGTTTCCGG TCAAGAGTGT CGTTCCAGCT TTGATAACAT TACTGCAGAT GGAGCATAAT 
TTCGATATTA TGAACCATGC TTGTCGAGCC TTAACATATA TGATGGAAGC ACTTCCTCGG 
TCATCTGCTG TTGTAGTAGA TGCAATTCCT GTCTTTTTAG AAAAGCTGCA AGTTATTCAG 
TGTATTGATG TCGCAGAGCA GGCCCTGACT GCCTTGGAGA TGTTATCACG CAGACACAGT 
AAAGCCATTC TACAGGCGGG TGGTTTGGCA GACTGCTTGC TGTATCTAGA ATTCTTCAGC 
ATAAATGCCC AAAGAAATGC ACTAGCAATT GCAGCCAATT GCTGCCAGAG TATCACGCCA 
GATGAATTTC ATTTTGTGGC AGATTCCCTC CCGTTGCTTA CCCAAAGGCT AACCCATCAG 
GACAAAAAGT CAGTTGAAAG CACTTGCCTT TGTTTTGCAC GTCTAGTGGA CAACTTCCAA 
CATGAGGAGA ATTTACTCCA GCAGGTTGCC TCCAAAGATC TGCTTACGAA TGTTCAACAA 
CTCTTGGTAG TGACTCCACC CATTTTAAGT TCTGGGATGT TTATAATGGT GGTTCGCATG 
TTTTCTCTGA TGTGTTCCAA CTGTCCAACT TTAGCTGTTC AACTTATGAA GCAAAACATT 
GCAGAAACAC TTCACTTTCT CCTGTGTGGT GCCTCTAATG GAAGTTGTCA GGAACAGATT 
GATCTTGTTC CACGAAGTCC ACAAGAACTG TATGAACTGA CATCTCTGAT TTGCGAACTT 
ATGCCATGTT TACCAAAAGA AGGCATTTTT GCAGTTGATA CCATGCTGAA GAAGGGAAAT 
GCGCAGAACA CAGACGGTGC GATTTGGCAG TGGCGTGATG ACCGTGGCCT TTGGCATCCC 
TATAACAGGA TTGACAGCCG GATCATTGAG GCAGCCCATC AGGTCGGTGA GGATGAGATA 
AGCTTGTCCA CTCTGGGACG AGTTTATACT ATTGATTTTA ATTCTATGCA GCAAATCAAT 
GAGGACACGG GAACAGCACG TGCCATTCAG AGAAAACCTA ACCCCTTAGC CAATACTAAC 
ACTAGTGGAT ATTCAGAGTT AAAGAAGGAT GATGCTCGAG CACAGCTTAT GAAAGAGGAT 
CCGGAACTGG CTAAGTCTTT TATTAAGACA TTATTTGGTG TTCTTTATGA AGTGTATAGT 
TCCTCAGCAG GACCTGCGGT CAGACATAAG TGCCTTAGAG CAATTCTTAG GATAATTTAT 
TTTGCGGATG CTGAACTTCT GAAGGATGTC CTGAAAAATC ATGCTGTTTC AAGTCACATT 
GCTTCCATGC TGTCAAGCCA AGACCTGAAG ATAGTAGTTG GAGCACTTCA GATGGCAGAA 
ATCTTAATGC AGAAGTTACC TGATATTTTT AGTGTTTACT TCAGAAGAGA AGGTGTAATG 
CATCAAGTAA AACACTTAGC AGAATCAGAG TCTTTGTTGA CAAGTCCACC AAAGGCATGT 
ACGAATGGGT CAGGATCCCT GGGATCCACG ACGTCAGTCA GCAGTGGAAC AGCCACGGCC 
GCCACCAATG CTGCAGCTGA CCTGGGGTCC CCCAGCTTGC AGCACAGCAG AGATGACTCT 
TTAGATCTGA GCCCTCAAGG TCGATTAAGT GATGTTCTAA AGAGAAAACG GCTGCCAAAA 
CGAGGGCCAA GAAGGCCAAA GTATTCACCT CCAAGAGATG ATGACAAAGT AGACAATCAA 
GCTAAAAGCC CTACCACTAC TCAGTCACCT AAATCTTCTT TCCTGGCAAG CTTGAATCCA 
AAAACGTGGG GAAGGTTAAG CGCACAGTCC AACAGCAACA ACATCGAACC AGCACGAACT 
GCGGGAGTCA GTGGTCTTGC CAGGGCCGCC TCCAAGGACA CCATATCCAA TAACAGAGAA 
AAAATTAAAG GTTGGATTAA GGAACAGGCG CATAAATTTG TAGAGCGTTA TTTCAGTTCT 
GAGAACATGG ATGGAAGCAA CCCTGCACTG AATGTCCTTC AGAGACTTTG TGCTGCAACT 
GAACAACTCA ACCTCCAGGT GGATGGTGGA GCTGAGTGCC TTGTAGAAAT CCGTAGCATA 
GTCTCAGAGT CTGATGTTTC ATCATTTGAA ATCCAACATA GTGGATTTGT GAAGCAGCTG 
TTACTTTATT TGACATCTAA AAGTGAAAAG GATGCTGTGA GCCGAGAGAT CAGATTAAAG 
AGATTCCTTC ACGTATTTTT TTCTTCACCA CTTCCTGGAG AAGAGCCCAT TGGAAGAGTA 
GAACCAGTGG GTAATGCGCC TTTGTTGGCA TTAGTTCACA AGATGAACAA CTGCCTCAGC 
CAGATGGAAC AGTTTCCAGT CAAAGTACAT GACTTCCCCA GTGGGAACGG GACAGGAGGC 
AGCTTTTCTC TCAACAGAGG ATCACAGGCT TTAAAATTTT TCAACACACA CCAGTTAAAA 
TGCCAGTTAC AAAGACATCC AGACTGTGCA AATGTGAAGC AGTGGAAGGG CGGGCCGGTC 
AAGATTGATC CCCTGGCTTT GGTACAAGCT ATTGAGAGAT ACCTTGTAGT TAGAGGGTAT 
GGAAGAGTAA GAGAAGATGA TGAAGACAGT GATGATGATG GCTCTGATGA GGAAATAGAC 
GAGTCTCTGG CCGCTCAGTT CTTAAATTCC GGAAACGTAA GGCACAGGCT ACAGTTTTAT 
ATTGGAGAAC ATTTGCTACC ATATAACATG ACTGTATATC AGGCAGTCCG GCAGTTCAGT 
ATACAAGCTG AGGATGAGCG GGAGTCCACA GATGATGAGA GCAACCCTCT GGGAAGAGCT 
GGTATTTGGA CAAAGACTCA TACAATATGG TACAAACCTG TGAGAGAGGA TGAAGAAAGT 
AATAAAGATT GTGTTGGTGG TAAAAGAGGA AGAGCCCAAA CGGCTCCAAC AAAAACATCC 
CCTAGAAATG CAAAAAAGCA TGATGAGTTA TGGCATGATG GAGTATGCCC ATCAGTATCC 
AATCCTTTAG AAGTTTACCT CATCTCCTCG CCACCTGAGA ATATAACTTT TGAAGACCCG 
TCCTTAGATG TGATTCTTCT TCTAAGAGTT TTACATGCCA TCAGCCGATA CTGGTATTAC 
TTGTATGATA ATGCAATGTG CAAGGAGATT ATTCCAACTA GTGAATTTAT TAACAGTAAG 
TTGACAGCAA AAGCAAATAG GCAGCTTCAA GATCCTTTAG TAATCATGAC AGGAAATATC 
CCAACGTGGC TTACTGAACT AGGAAAAACC TGCCCATTTT TCTTCCCTTT TGATACCCGG 
CAAATGCTTT TTTACGTAAC TGCATTTGAT CGGGACCGAG CAATGCAAAG ATTACTTGAT 
ACCAACCCAG AAATCAACCA GTCGGATTCT CAAGATAGCA GAGTTGCACC TAGATTGGAT 
AGAAAAAAAC GTACTGTGAA CAGAGAGGAG CTGCTGAAAC AAGCGGAGTC TGTAATGCAG 
GATCTCGGTA GCTCACGGGC CATGTTAGAA ATCCAGTATG AAAATGAGGT TGGTACAGGT 
CTTGGGCCTA CACTGGAGTT TTATGCACTT GTATCTCAGG AACTTCAGAG AGCTGACTTG 
GGTCTCTGGA GAGGTGAAGA AGTGACTCTC AGCAATCCAA AAGGAAGCCA AGAAGGGACC 
AAGTATATTC AAAACCTCCA GGGCCTGTTT GCACTTCCCT TTGGTAGGAC AGCTAAGCCA 
GCCCATATCG CAAAGGTTAA AATGAAGTTT CGCTTCTTAG GAAAATTAAT GGCCAAGGCT 
ATCATGGATT TCAGATTGGT GGACCTTCCC CTTGGTTTAC CCTTTTATAA GTGGATGCTA 
AGGCAAGAAA CTTCACTGAC GTCGCATGAT TTGTTTGACA TTGACCCAGT TGTAGCCAGA 
TCAGTGTATC ACCTAGAAGA CATTGTCAGA CAGAAGAAAA GACTTGAGCA AGATAAATCC 
CAGACCAAAG AGAGTCTACA ATATGCATTA GAAACCCTGA CTATGAATGG CTGCTCAGTT 
GAAGATCTAG GATTGGATTT CACGCTGCCA GGGTTTCCCA ACATTGAACT GAAGAAAGGA 
GGAAAGGATA TACCAGTTAC CATCCACAAT TTAGAGGAGT ATCTAAGACT GGTTATATTC 
TGGGCACTAA ATGAAGGCGT TTCCAGGCAA TTTGATTCCT TCAGAGATGG ATTTGAATCT 
GTCTTCCCAC TTAGTCATCT TCAGTACTTC TACCCGGAAG AACTGGATCA GCTCTTGTGC 
GGCAGTAAAG CAGACACGTG GGATGCGAAG ACACTGATGG AGTGCTGCAG GCCGGACCAC 
GGTTATACTC ATGACAGTCG GGCTGTGAAG TTCTTGTTTG AGATTCTCAG TAGTTTTGAT 
AATGAGCAGC AGAGGTTGTT TCTCCAGTTT GTGACAGGTA GCCCAAGATT GCCTGTTGGA 
GGCTTCCGGA GTCTGAATCC ACCTCTGACA ATTGTCCGGA AGACCTTCGA GTCCACGGAG 
AACCCCGACG ACTTCTTGCC CTCTGTAATG ACTTGTGTGA ACTACCTTAA GTTGCCGGAC 
TACTCGAGCA TTGACATCAT GCGTGAGAAA CTGTTGATTG CAGCGAGAGA AGGGCAGCAG 
TCATTCCATC TTTCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_022265380.1
CDS	1..6078
Translation	MSNRPNNNPGGSLRRSQRNTAGAQPQDDSIGGRSCSSSSAVIVPQPEDPDRANTSERQKTGQVPKKDNSRGVKRSASPDYNRTNSPSSAKKPKALQHTESPSETNKPHSKSKKRHLDQEQQLKSAQSPSTSKAHTRKSGATGGSRSQKRKRTESSCLKSGSVSESTGAEERSAKPTKLASKSAASAKAGCSTITDSSSAASTSSSSSAVASASSTVPPGARVKQGKDQSKARRSRSASSPSPRRSSREKEQSKTGGSSKFDWAARFSPKVSLPKTKLSLPGSSKSETSKPGPSGLQAKLASLRKSTKKRSESPPAELPSLRRSTRQKTTGSCASASRRGSGLGKRGAAEARRQEKMADPESSQETVNSSAARTDETPQGAAASSSVAGAVGMTTSGESESDDSEMGRLQALLEARGLPPHLFGPLGPRMSQLFHRTIGSGASSKAQQLLQGLQASDESQQLQAVIEMCQLLVMGNEETLGGFPVKSVVPALITLLQMEHNFDIMNHACRALTYMMEALPRSSAVVVDAIPVFLEKLQVIQCIDVAEQALTALEMLSRRHSKAILQAGGLADCLLYLEFFSINAQRNALAIAANCCQSITPDEFHFVADSLPLLTQRLTHQDKKSVESTCLCFARLVDNFQHEENLLQQVASKDLLTNVQQLLVVTPPILSSGMFIMVVRMFSLMCSNCPTLAVQLMKQNIAETLHFLLCGASNGSCQEQIDLVPRSPQELYELTSLICELMPCLPKEGIFAVDTMLKKGNAQNTDGAIWQWRDDRGLWHPYNRIDSRIIEAAHQVGEDEISLSTLGRVYTIDFNSMQQINEDTGTARAIQRKPNPLANTNTSGYSELKKDDARAQLMKEDPELAKSFIKTLFGVLYEVYSSSAGPAVRHKCLRAILRIIYFADAELLKDVLKNHAVSSHIASMLSSQDLKIVVGALQMAEILMQKLPDIFSVYFRREGVMHQVKHLAESESLLTSPPKACTNGSGSLGSTTSVSSGTATAATNAAADLGSPSLQHSRDDSLDLSPQGRLSDVLKRKRLPKRGPRRPKYSPPRDDDKVDNQAKSPTTTQSPKSSFLASLNPKTWGRLSAQSNSNNIEPARTAGVSGLARAASKDTISNNREKIKGWIKEQAHKFVERYFSSENMDGSNPALNVLQRLCAATEQLNLQVDGGAECLVEIRSIVSESDVSSFEIQHSGFVKQLLLYLTSKSEKDAVSREIRLKRFLHVFFSSPLPGEEPIGRVEPVGNAPLLALVHKMNNCLSQMEQFPVKVHDFPSGNGTGGSFSLNRGSQALKFFNTHQLKCQLQRHPDCANVKQWKGGPVKIDPLALVQAIERYLVVRGYGRVREDDEDSDDDGSDEEIDESLAAQFLNSGNVRHRLQFYIGEHLLPYNMTVYQAVRQFSIQAEDERESTDDESNPLGRAGIWTKTHTIWYKPVREDEESNKDCVGGKRGRAQTAPTKTSPRNAKKHDELWHDGVCPSVSNPLEVYLISSPPENITFEDPSLDVILLLRVLHAISRYWYYLYDNAMCKEIIPTSEFINSKLTAKANRQLQDPLVIMTGNIPTWLTELGKTCPFFFPFDTRQMLFYVTAFDRDRAMQRLLDTNPEINQSDSQDSRVAPRLDRKKRTVNREELLKQAESVMQDLGSSRAMLEIQYENEVGTGLGPTLEFYALVSQELQRADLGLWRGEEVTLSNPKGSQEGTKYIQNLQGLFALPFGRTAKPAHIAKVKMKFRFLGKLMAKAIMDFRLVDLPLGLPFYKWMLRQETSLTSHDLFDIDPVVARSVYHLEDIVRQKKRLEQDKSQTKESLQYALETLTMNGCSVEDLGLDFTLPGFPNIELKKGGKDIPVTIHNLEEYLRLVIFWALNEGVSRQFDSFRDGFESVFPLSHLQYFYPEELDQLLCGSKADTWDAKTLMECCRPDHGYTHDSRAVKFLFEILSSFDNEQQRLFLQFVTGSPRLPVGGFRSLNPPLTIVRKTFESTENPDDFLPSVMTCVNYLKLPDYSSIDIMREKLLIAAREGQQSFHLS

Target ORF information:

RefSeq Version	XM_022409672.1
Organism	Canis lupus familiaris(dog)
Definition	Canis lupus familiaris thyroid hormone receptor interactor 12 (TRIP12), transcript variant X13, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_022409672.1

ORF Insert Sequence:

ATGTCCAACC GGCCTAATAA CAATCCAGGG GGGTCACTGC GACGTTCACA GAGGAACACT 
GCCGGGGCCC AACCACAAGA CGACTCGATA GGAGGAAGAA GCTGCAGTTC ATCATCTGCT 
GTCATAGTTC CACAACCAGA GGATCCAGAC AGAGCCAATA CTTCAGAAAG GCAAAAAACG 
GGGCAGGTGC CTAAGAAAGA CAATTCTCGA GGAGTGAAAC GCAGTGCTAG TCCAGACTAC 
AACAGGACCA ATTCTCCTAG CTCTGCCAAA AAACCAAAAG CACTTCAGCA TACTGAATCT 
CCCTCAGAAA CAAATAAGCC ACATAGTAAG TCGAAGAAGA GACATTTAGA CCAGGAGCAA 
CAACTGAAAT CTGCACAGTC GCCATCAACA AGTAAGGCTC ACACCAGAAA GAGTGGGGCC 
ACCGGCGGCT CACGGAGTCA GAAAAGAAAG AGGACAGAGA GTTCTTGTCT AAAGAGTGGT 
TCTGTGTCTG AGTCAACTGG TGCCGAAGAG AGATCTGCAA AACCCACCAA GCTGGCTTCA 
AAATCCGCCG CCTCGGCCAA AGCTGGGTGC AGCACCATCA CTGATTCTTC CTCCGCTGCC 
TCTACTTCCT CCTCATCTTC TGCTGTCGCC TCGGCCTCCT CCACTGTACC ACCAGGGGCC 
AGGGTGAAAC AAGGAAAAGA CCAGAGCAAG GCCAGGCGTT CCCGTTCAGC ATCCAGTCCC 
AGTCCCAGAA GAAGTAGCAG GGAAAAGGAA CAGAGTAAAA CTGGTGGCTC TTCAAAATTC 
GATTGGGCTG CTCGTTTCAG CCCTAAAGTT AGCCTTCCTA AAACCAAACT GTCTCTTCCA 
GGGTCTTCTA AGTCAGAGAC ATCAAAACCT GGACCTTCTG GATTACAGGC CAAATTAGCA 
AGTTTAAGAA AATCTACCAA GAAGCGCAGT GAATCCCCAC CTGCTGAGCT CCCCAGTTTG 
AGGCGGAGCA CACGCCAAAA GACCACGGGC TCCTGTGCTA GTGCCAGTCG GCGAGGCTCT 
GGCCTGGGCA AAAGAGGAGC AGCTGAAGCT CGTCGACAGG AGAAGATGGC GGACCCTGAA 
AGCAGCCAGG AGACCGTGAA TTCTTCAGCT GCACGGACAG ACGAAACTCC CCAAGGAGCT 
GCAGCCTCTA GTTCTGTTGC AGGGGCTGTG GGCATGACCA CCTCTGGGGA GAGTGAATCC 
GATGATTCTG AGATGGGACG ACTACAAGCT TTGTTAGAGG CCAGGGGTCT TCCTCCTCAT 
CTGTTTGGCC CTCTCGGTCC TCGGATGTCA CAACTTTTCC ATAGAACAAT TGGAAGCGGA 
GCTAGCTCTA AGGCCCAGCA GCTTCTGCAA GGACTACAAG CAAGTGATGA AAGCCAGCAA 
CTCCAGGCAG TTATTGAAAT GTGTCAGTTA TTGGTTATGG GAAATGAGGA GACGCTGGGA 
GGGTTTCCGG TCAAGAGTGT CGTTCCAGCT TTGATAACAT TACTGCAGAT GGAGCATAAT 
TTCGATATTA TGAACCATGC TTGTCGAGCC TTAACATATA TGATGGAAGC ACTTCCTCGG 
TCATCTGCTG TTGTAGTAGA TGCAATTCCT GTCTTTTTAG AAAAGCTGCA AGTTATTCAG 
TGTATTGATG TCGCAGAGCA GGCCCTGACT GCCTTGGAGA TGTTATCACG CAGACACAGT 
AAAGCCATTC TACAGGCGGG TGGTTTGGCA GACTGCTTGC TGTATCTAGA ATTCTTCAGC 
ATAAATGCCC AAAGAAATGC ACTAGCAATT GCAGCCAATT GCTGCCAGAG TATCACGCCA 
GATGAATTTC ATTTTGTGGC AGATTCCCTC CCGTTGCTTA CCCAAAGGCT AACCCATCAG 
GACAAAAAGT CAGTTGAAAG CACTTGCCTT TGTTTTGCAC GTCTAGTGGA CAACTTCCAA 
CATGAGGAGA ATTTACTCCA GCAGGTTGCC TCCAAAGATC TGCTTACGAA TGTTCAACAA 
CTCTTGGTAG TGACTCCACC CATTTTAAGT TCTGGGATGT TTATAATGGT GGTTCGCATG 
TTTTCTCTGA TGTGTTCCAA CTGTCCAACT TTAGCTGTTC AACTTATGAA GCAAAACATT 
GCAGAAACAC TTCACTTTCT CCTGTGTGGT GCCTCTAATG GAAGTTGTCA GGAACAGATT 
GATCTTGTTC CACGAAGTCC ACAAGAACTG TATGAACTGA CATCTCTGAT TTGCGAACTT 
ATGCCATGTT TACCAAAAGA AGGCATTTTT GCAGTTGATA CCATGCTGAA GAAGGGAAAT 
GCGCAGAACA CAGACGGTGC GATTTGGCAG TGGCGTGATG ACCGTGGCCT TTGGCATCCC 
TATAACAGGA TTGACAGCCG GATCATTGAG GCAGCCCATC AGGTCGGTGA GGATGAGATA 
AGCTTGTCCA CTCTGGGACG AGTTTATACT ATTGATTTTA ATTCTATGCA GCAAATCAAT 
GAGGACACGG GAACAGCACG TGCCATTCAG AGAAAACCTA ACCCCTTAGC CAATACTAAC 
ACTAGTGGAT ATTCAGAGTT AAAGAAGGAT GATGCTCGAG CACAGCTTAT GAAAGAGGAT 
CCGGAACTGG CTAAGTCTTT TATTAAGACA TTATTTGGTG TTCTTTATGA AGTGTATAGT 
TCCTCAGCAG GACCTGCGGT CAGACATAAG TGCCTTAGAG CAATTCTTAG GATAATTTAT 
TTTGCGGATG CTGAACTTCT GAAGGATGTC CTGAAAAATC ATGCTGTTTC AAGTCACATT 
GCTTCCATGC TGTCAAGCCA AGACCTGAAG ATAGTAGTTG GAGCACTTCA GATGGCAGAA 
ATCTTAATGC AGAAGTTACC TGATATTTTT AGTGTTTACT TCAGAAGAGA AGGTGTAATG 
CATCAAGTAA AACACTTAGC AGAATCAGAG TCTTTGTTGA CAAGTCCACC AAAGGCATGT 
ACGAATGGGT CAGGATCCCT GGGATCCACG ACGTCAGTCA GCAGTGGAAC AGCCACGGCC 
GCCACCAATG CTGCAGCTGA CCTGGGGTCC CCCAGCTTGC AGCACAGCAG AGATGACTCT 
TTAGATCTGA GCCCTCAAGG TCGATTAAGT GATGTTCTAA AGAGAAAACG GCTGCCAAAA 
CGAGGGCCAA GAAGGCCAAA GTATTCACCT CCAAGAGATG ATGACAAAGT AGACAATCAA 
GCTAAAAGCC CTACCACTAC TCAGTCACCT AAATCTTCTT TCCTGGCAAG CTTGAATCCA 
AAAACGTGGG GAAGGTTAAG CGCACAGTCC AACAGCAACA ACATCGAACC AGCACGAACT 
GCGGGAGTCA GTGGTCTTGC CAGGGCCGCC TCCAAGGACA CCATATCCAA TAACAGAGAA 
AAAATTAAAG GTTGGATTAA GGAACAGGCG CATAAATTTG TAGAGCGTTA TTTCAGTTCT 
GAGAACATGG ATGGAAGCAA CCCTGCACTG AATGTCCTTC AGAGACTTTG TGCTGCAACT 
GAACAACTCA ACCTCCAGGT GGATGGTGGA GCTGAGTGCC TTGTAGAAAT CCGTAGCATA 
GTCTCAGAGT CTGATGTTTC ATCATTTGAA ATCCAACATA GTGGATTTGT GAAGCAGCTG 
TTACTTTATT TGACATCTAA AAGTGAAAAG GATGCTGTGA GCCGAGAGAT CAGATTAAAG 
AGATTCCTTC ACGTATTTTT TTCTTCACCA CTTCCTGGAG AAGAGCCCAT TGGAAGAGTA 
GAACCAGTGG GTAATGCGCC TTTGTTGGCA TTAGTTCACA AGATGAACAA CTGCCTCAGC 
CAGATGGAAC AGTTTCCAGT CAAAGTACAT GACTTCCCCA GTGGGAACGG GACAGGAGGC 
AGCTTTTCTC TCAACAGAGG ATCACAGGCT TTAAAATTTT TCAACACACA CCAGTTAAAA 
TGCCAGTTAC AAAGACATCC AGACTGTGCA AATGTGAAGC AGTGGAAGGG CGGGCCGGTC 
AAGATTGATC CCCTGGCTTT GGTACAAGCT ATTGAGAGAT ACCTTGTAGT TAGAGGGTAT 
GGAAGAGTAA GAGAAGATGA TGAAGACAGT GATGATGATG GCTCTGATGA GGAAATAGAC 
GAGTCTCTGG CCGCTCAGTT CTTAAATTCC GGAAACGTAA GGCACAGGCT ACAGTTTTAT 
ATTGGAGAAC ATTTGCTACC ATATAACATG ACTGTATATC AGGCAGTCCG GCAGTTCAGT 
ATACAAGCTG AGGATGAGCG GGAGTCCACA GATGATGAGA GCAACCCTCT GGGAAGAGCT 
GGTATTTGGA CAAAGACTCA TACAATATGG TACAAACCTG TGAGAGAGGA TGAAGAAAGT 
AATAAAGATT GTGTTGGTGG TAAAAGAGGA AGAGCCCAAA CGGCTCCAAC AAAAACATCC 
CCTAGAAATG CAAAAAAGCA TGATGAGTTA TGGCATGATG GAGTATGCCC ATCAGTATCC 
AATCCTTTAG AAGTTTACCT CATCTCCTCG CCACCTGAGA ATATAACTTT TGAAGACCCG 
TCCTTAGATG TGATTCTTCT TCTAAGAGTT TTACATGCCA TCAGCCGATA CTGGTATTAC 
TTGTATGATA ATGCAATGTG CAAGGAGATT ATTCCAACTA GTGAATTTAT TAACAGTAAG 
TTGACAGCAA AAGCAAATAG GCAGCTTCAA GATCCTTTAG TAATCATGAC AGGAAATATC 
CCAACGTGGC TTACTGAACT AGGAAAAACC TGCCCATTTT TCTTCCCTTT TGATACCCGG 
CAAATGCTTT TTTACGTAAC TGCATTTGAT CGGGACCGAG CAATGCAAAG ATTACTTGAT 
ACCAACCCAG AAATCAACCA GTCGGATTCT CAAGATAGCA GAGTTGCACC TAGATTGGAT 
AGAAAAAAAC GTACTGTGAA CAGAGAGGAG CTGCTGAAAC AAGCGGAGTC TGTAATGCAG 
GATCTCGGTA GCTCACGGGC CATGTTAGAA ATCCAGTATG AAAATGAGGT TGGTACAGGT 
CTTGGGCCTA CACTGGAGTT TTATGCACTT GTATCTCAGG AACTTCAGAG AGCTGACTTG 
GGTCTCTGGA GAGGTGAAGA AGTGACTCTC AGCAATCCAA AAGGAAGCCA AGAAGGGACC 
AAGTATATTC AAAACCTCCA GGGCCTGTTT GCACTTCCCT TTGGTAGGAC AGCTAAGCCA 
GCCCATATCG CAAAGGTTAA AATGAAGTTT CGCTTCTTAG GAAAATTAAT GGCCAAGGCT 
ATCATGGATT TCAGATTGGT GGACCTTCCC CTTGGTTTAC CCTTTTATAA GTGGATGCTA 
AGGCAAGAAA CTTCACTGAC GTCGCATGAT TTGTTTGACA TTGACCCAGT TGTAGCCAGA 
TCAGTGTATC ACCTAGAAGA CATTGTCAGA CAGAAGAAAA GACTTGAGCA AGATAAATCC 
CAGACCAAAG AGAGTCTACA ATATGCATTA GAAACCCTGA CTATGAATGG CTGCTCAGTT 
GAAGATCTAG GATTGGATTT CACGCTGCCA GGGTTTCCCA ACATTGAACT GAAGAAAGGA 
GGAAAGGATA TACCAGTTAC CATCCACAAT TTAGAGGAGT ATCTAAGACT GGTTATATTC 
TGGGCACTAA ATGAAGGCGT TTCCAGGCAA TTTGATTCCT TCAGAGATGG ATTTGAATCT 
GTCTTCCCAC TTAGTCATCT TCAGTACTTC TACCCGGAAG AACTGGATCA GCTCTTGTGC 
GGCAGTAAAG CAGACACGTG GGATGCGAAG ACACTGATGG AGTGCTGCAG GCCGGACCAC 
GGTTATACTC ATGACAGTCG GGCTGTGAAG TTCTTGTTTG AGATTCTCAG TAGTTTTGAT 
AATGAGCAGC AGAGGTTGTT TCTCCAGTTT GTGACAGGTA GCCCAAGATT GCCTGTTGGA 
GGCTTCCGGA GTCTGAATCC ACCTCTGACA ATTGTCCGGA AGACCTTCGA GTCCACGGAG 
AACCCCGACG ACTTCTTGCC CTCTGTAATG ACTTGTGTGA ACTACCTTAA GTTGCCGGAC 
TACTCGAGCA TTGACATCAT GCGTGAGAAA CTGTTGATTG CAGCGAGAGA AGGGCAGCAG 
TCATTCCATC TTTCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TRIP12 ( XM_005635809.2 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_005635866.1 Canis lupus familiaris thyroid hormone receptor interactor 12 (TRIP12), transcript variant X9, mRNA.

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CloneID	OCe25769
Clone ID Related Accession (Same CDS sequence)	XM_022409672.1 , XM_005635809.2
Accession Version	XM_005635809.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6078bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-09-16
Organism	Canis lupus familiaris(Dog)
Product	E3 ubiquitin-protein ligase TRIP12 isoform X8
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726103.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 17, 2015 this sequence version replaced XM_005635809.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCCAACC GGCCTAATAA CAATCCAGGG GGGTCACTGC GACGTTCACA GAGGAACACT 
GCCGGGGCCC AACCACAAGA CGACTCGATA GGAGGAAGAA GCTGCAGTTC ATCATCTGCT 
GTCATAGTTC CACAACCAGA GGATCCAGAC AGAGCCAATA CTTCAGAAAG GCAAAAAACG 
GGGCAGGTGC CTAAGAAAGA CAATTCTCGA GGAGTGAAAC GCAGTGCTAG TCCAGACTAC 
AACAGGACCA ATTCTCCTAG CTCTGCCAAA AAACCAAAAG CACTTCAGCA TACTGAATCT 
CCCTCAGAAA CAAATAAGCC ACATAGTAAG TCGAAGAAGA GACATTTAGA CCAGGAGCAA 
CAACTGAAAT CTGCACAGTC GCCATCAACA AGTAAGGCTC ACACCAGAAA GAGTGGGGCC 
ACCGGCGGCT CACGGAGTCA GAAAAGAAAG AGGACAGAGA GTTCTTGTCT AAAGAGTGGT 
TCTGTGTCTG AGTCAACTGG TGCCGAAGAG AGATCTGCAA AACCCACCAA GCTGGCTTCA 
AAATCCGCCG CCTCGGCCAA AGCTGGGTGC AGCACCATCA CTGATTCTTC CTCCGCTGCC 
TCTACTTCCT CCTCATCTTC TGCTGTCGCC TCGGCCTCCT CCACTGTACC ACCAGGGGCC 
AGGGTGAAAC AAGGAAAAGA CCAGAGCAAG GCCAGGCGTT CCCGTTCAGC ATCCAGTCCC 
AGTCCCAGAA GAAGTAGCAG GGAAAAGGAA CAGAGTAAAA CTGGTGGCTC TTCAAAATTC 
GATTGGGCTG CTCGTTTCAG CCCTAAAGTT AGCCTTCCTA AAACCAAACT GTCTCTTCCA 
GGGTCTTCTA AGTCAGAGAC ATCAAAACCT GGACCTTCTG GATTACAGGC CAAATTAGCA 
AGTTTAAGAA AATCTACCAA GAAGCGCAGT GAATCCCCAC CTGCTGAGCT CCCCAGTTTG 
AGGCGGAGCA CACGCCAAAA GACCACGGGC TCCTGTGCTA GTGCCAGTCG GCGAGGCTCT 
GGCCTGGGCA AAAGAGGAGC AGCTGAAGCT CGTCGACAGG AGAAGATGGC GGACCCTGAA 
AGCAGCCAGG AGACCGTGAA TTCTTCAGCT GCACGGACAG ACGAAACTCC CCAAGGAGCT 
GCAGCCTCTA GTTCTGTTGC AGGGGCTGTG GGCATGACCA CCTCTGGGGA GAGTGAATCC 
GATGATTCTG AGATGGGACG ACTACAAGCT TTGTTAGAGG CCAGGGGTCT TCCTCCTCAT 
CTGTTTGGCC CTCTCGGTCC TCGGATGTCA CAACTTTTCC ATAGAACAAT TGGAAGCGGA 
GCTAGCTCTA AGGCCCAGCA GCTTCTGCAA GGACTACAAG CAAGTGATGA AAGCCAGCAA 
CTCCAGGCAG TTATTGAAAT GTGTCAGTTA TTGGTTATGG GAAATGAGGA GACGCTGGGA 
GGGTTTCCGG TCAAGAGTGT CGTTCCAGCT TTGATAACAT TACTGCAGAT GGAGCATAAT 
TTCGATATTA TGAACCATGC TTGTCGAGCC TTAACATATA TGATGGAAGC ACTTCCTCGG 
TCATCTGCTG TTGTAGTAGA TGCAATTCCT GTCTTTTTAG AAAAGCTGCA AGTTATTCAG 
TGTATTGATG TCGCAGAGCA GGCCCTGACT GCCTTGGAGA TGTTATCACG CAGACACAGT 
AAAGCCATTC TACAGGCGGG TGGTTTGGCA GACTGCTTGC TGTATCTAGA ATTCTTCAGC 
ATAAATGCCC AAAGAAATGC ACTAGCAATT GCAGCCAATT GCTGCCAGAG TATCACGCCA 
GATGAATTTC ATTTTGTGGC AGATTCCCTC CCGTTGCTTA CCCAAAGGCT AACCCATCAG 
GACAAAAAGT CAGTTGAAAG CACTTGCCTT TGTTTTGCAC GTCTAGTGGA CAACTTCCAA 
CATGAGGAGA ATTTACTCCA GCAGGTTGCC TCCAAAGATC TGCTTACGAA TGTTCAACAA 
CTCTTGGTAG TGACTCCACC CATTTTAAGT TCTGGGATGT TTATAATGGT GGTTCGCATG 
TTTTCTCTGA TGTGTTCCAA CTGTCCAACT TTAGCTGTTC AACTTATGAA GCAAAACATT 
GCAGAAACAC TTCACTTTCT CCTGTGTGGT GCCTCTAATG GAAGTTGTCA GGAACAGATT 
GATCTTGTTC CACGAAGTCC ACAAGAACTG TATGAACTGA CATCTCTGAT TTGCGAACTT 
ATGCCATGTT TACCAAAAGA AGGCATTTTT GCAGTTGATA CCATGCTGAA GAAGGGAAAT 
GCGCAGAACA CAGACGGTGC GATTTGGCAG TGGCGTGATG ACCGTGGCCT TTGGCATCCC 
TATAACAGGA TTGACAGCCG GATCATTGAG GCAGCCCATC AGGTCGGTGA GGATGAGATA 
AGCTTGTCCA CTCTGGGACG AGTTTATACT ATTGATTTTA ATTCTATGCA GCAAATCAAT 
GAGGACACGG GAACAGCACG TGCCATTCAG AGAAAACCTA ACCCCTTAGC CAATACTAAC 
ACTAGTGGAT ATTCAGAGTT AAAGAAGGAT GATGCTCGAG CACAGCTTAT GAAAGAGGAT 
CCGGAACTGG CTAAGTCTTT TATTAAGACA TTATTTGGTG TTCTTTATGA AGTGTATAGT 
TCCTCAGCAG GACCTGCGGT CAGACATAAG TGCCTTAGAG CAATTCTTAG GATAATTTAT 
TTTGCGGATG CTGAACTTCT GAAGGATGTC CTGAAAAATC ATGCTGTTTC AAGTCACATT 
GCTTCCATGC TGTCAAGCCA AGACCTGAAG ATAGTAGTTG GAGCACTTCA GATGGCAGAA 
ATCTTAATGC AGAAGTTACC TGATATTTTT AGTGTTTACT TCAGAAGAGA AGGTGTAATG 
CATCAAGTAA AACACTTAGC AGAATCAGAG TCTTTGTTGA CAAGTCCACC AAAGGCATGT 
ACGAATGGGT CAGGATCCCT GGGATCCACG ACGTCAGTCA GCAGTGGAAC AGCCACGGCC 
GCCACCAATG CTGCAGCTGA CCTGGGGTCC CCCAGCTTGC AGCACAGCAG AGATGACTCT 
TTAGATCTGA GCCCTCAAGG TCGATTAAGT GATGTTCTAA AGAGAAAACG GCTGCCAAAA 
CGAGGGCCAA GAAGGCCAAA GTATTCACCT CCAAGAGATG ATGACAAAGT AGACAATCAA 
GCTAAAAGCC CTACCACTAC TCAGTCACCT AAATCTTCTT TCCTGGCAAG CTTGAATCCA 
AAAACGTGGG GAAGGTTAAG CGCACAGTCC AACAGCAACA ACATCGAACC AGCACGAACT 
GCGGGAGTCA GTGGTCTTGC CAGGGCCGCC TCCAAGGACA CCATATCCAA TAACAGAGAA 
AAAATTAAAG GTTGGATTAA GGAACAGGCG CATAAATTTG TAGAGCGTTA TTTCAGTTCT 
GAGAACATGG ATGGAAGCAA CCCTGCACTG AATGTCCTTC AGAGACTTTG TGCTGCAACT 
GAACAACTCA ACCTCCAGGT GGATGGTGGA GCTGAGTGCC TTGTAGAAAT CCGTAGCATA 
GTCTCAGAGT CTGATGTTTC ATCATTTGAA ATCCAACATA GTGGATTTGT GAAGCAGCTG 
TTACTTTATT TGACATCTAA AAGTGAAAAG GATGCTGTGA GCCGAGAGAT CAGATTAAAG 
AGATTCCTTC ACGTATTTTT TTCTTCACCA CTTCCTGGAG AAGAGCCCAT TGGAAGAGTA 
GAACCAGTGG GTAATGCGCC TTTGTTGGCA TTAGTTCACA AGATGAACAA CTGCCTCAGC 
CAGATGGAAC AGTTTCCAGT CAAAGTACAT GACTTCCCCA GTGGGAACGG GACAGGAGGC 
AGCTTTTCTC TCAACAGAGG ATCACAGGCT TTAAAATTTT TCAACACACA CCAGTTAAAA 
TGCCAGTTAC AAAGACATCC AGACTGTGCA AATGTGAAGC AGTGGAAGGG CGGGCCGGTC 
AAGATTGATC CCCTGGCTTT GGTACAAGCT ATTGAGAGAT ACCTTGTAGT TAGAGGGTAT 
GGAAGAGTAA GAGAAGATGA TGAAGACAGT GATGATGATG GCTCTGATGA GGAAATAGAC 
GAGTCTCTGG CCGCTCAGTT CTTAAATTCC GGAAACGTAA GGCACAGGCT ACAGTTTTAT 
ATTGGAGAAC ATTTGCTACC ATATAACATG ACTGTATATC AGGCAGTCCG GCAGTTCAGT 
ATACAAGCTG AGGATGAGCG GGAGTCCACA GATGATGAGA GCAACCCTCT GGGAAGAGCT 
GGTATTTGGA CAAAGACTCA TACAATATGG TACAAACCTG TGAGAGAGGA TGAAGAAAGT 
AATAAAGATT GTGTTGGTGG TAAAAGAGGA AGAGCCCAAA CGGCTCCAAC AAAAACATCC 
CCTAGAAATG CAAAAAAGCA TGATGAGTTA TGGCATGATG GAGTATGCCC ATCAGTATCC 
AATCCTTTAG AAGTTTACCT CATCTCCTCG CCACCTGAGA ATATAACTTT TGAAGACCCG 
TCCTTAGATG TGATTCTTCT TCTAAGAGTT TTACATGCCA TCAGCCGATA CTGGTATTAC 
TTGTATGATA ATGCAATGTG CAAGGAGATT ATTCCAACTA GTGAATTTAT TAACAGTAAG 
TTGACAGCAA AAGCAAATAG GCAGCTTCAA GATCCTTTAG TAATCATGAC AGGAAATATC 
CCAACGTGGC TTACTGAACT AGGAAAAACC TGCCCATTTT TCTTCCCTTT TGATACCCGG 
CAAATGCTTT TTTACGTAAC TGCATTTGAT CGGGACCGAG CAATGCAAAG ATTACTTGAT 
ACCAACCCAG AAATCAACCA GTCGGATTCT CAAGATAGCA GAGTTGCACC TAGATTGGAT 
AGAAAAAAAC GTACTGTGAA CAGAGAGGAG CTGCTGAAAC AAGCGGAGTC TGTAATGCAG 
GATCTCGGTA GCTCACGGGC CATGTTAGAA ATCCAGTATG AAAATGAGGT TGGTACAGGT 
CTTGGGCCTA CACTGGAGTT TTATGCACTT GTATCTCAGG AACTTCAGAG AGCTGACTTG 
GGTCTCTGGA GAGGTGAAGA AGTGACTCTC AGCAATCCAA AAGGAAGCCA AGAAGGGACC 
AAGTATATTC AAAACCTCCA GGGCCTGTTT GCACTTCCCT TTGGTAGGAC AGCTAAGCCA 
GCCCATATCG CAAAGGTTAA AATGAAGTTT CGCTTCTTAG GAAAATTAAT GGCCAAGGCT 
ATCATGGATT TCAGATTGGT GGACCTTCCC CTTGGTTTAC CCTTTTATAA GTGGATGCTA 
AGGCAAGAAA CTTCACTGAC GTCGCATGAT TTGTTTGACA TTGACCCAGT TGTAGCCAGA 
TCAGTGTATC ACCTAGAAGA CATTGTCAGA CAGAAGAAAA GACTTGAGCA AGATAAATCC 
CAGACCAAAG AGAGTCTACA ATATGCATTA GAAACCCTGA CTATGAATGG CTGCTCAGTT 
GAAGATCTAG GATTGGATTT CACGCTGCCA GGGTTTCCCA ACATTGAACT GAAGAAAGGA 
GGAAAGGATA TACCAGTTAC CATCCACAAT TTAGAGGAGT ATCTAAGACT GGTTATATTC 
TGGGCACTAA ATGAAGGCGT TTCCAGGCAA TTTGATTCCT TCAGAGATGG ATTTGAATCT 
GTCTTCCCAC TTAGTCATCT TCAGTACTTC TACCCGGAAG AACTGGATCA GCTCTTGTGC 
GGCAGTAAAG CAGACACGTG GGATGCGAAG ACACTGATGG AGTGCTGCAG GCCGGACCAC 
GGTTATACTC ATGACAGTCG GGCTGTGAAG TTCTTGTTTG AGATTCTCAG TAGTTTTGAT 
AATGAGCAGC AGAGGTTGTT TCTCCAGTTT GTGACAGGTA GCCCAAGATT GCCTGTTGGA 
GGCTTCCGGA GTCTGAATCC ACCTCTGACA ATTGTCCGGA AGACCTTCGA GTCCACGGAG 
AACCCCGACG ACTTCTTGCC CTCTGTAATG ACTTGTGTGA ACTACCTTAA GTTGCCGGAC 
TACTCGAGCA TTGACATCAT GCGTGAGAAA CTGTTGATTG CAGCGAGAGA AGGGCAGCAG 
TCATTCCATC TTTCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005635866.1
CDS	188..6265
Translation	MSNRPNNNPGGSLRRSQRNTAGAQPQDDSIGGRSCSSSSAVIVPQPEDPDRANTSERQKTGQVPKKDNSRGVKRSASPDYNRTNSPSSAKKPKALQHTESPSETNKPHSKSKKRHLDQEQQLKSAQSPSTSKAHTRKSGATGGSRSQKRKRTESSCLKSGSVSESTGAEERSAKPTKLASKSAASAKAGCSTITDSSSAASTSSSSSAVASASSTVPPGARVKQGKDQSKARRSRSASSPSPRRSSREKEQSKTGGSSKFDWAARFSPKVSLPKTKLSLPGSSKSETSKPGPSGLQAKLASLRKSTKKRSESPPAELPSLRRSTRQKTTGSCASASRRGSGLGKRGAAEARRQEKMADPESSQETVNSSAARTDETPQGAAASSSVAGAVGMTTSGESESDDSEMGRLQALLEARGLPPHLFGPLGPRMSQLFHRTIGSGASSKAQQLLQGLQASDESQQLQAVIEMCQLLVMGNEETLGGFPVKSVVPALITLLQMEHNFDIMNHACRALTYMMEALPRSSAVVVDAIPVFLEKLQVIQCIDVAEQALTALEMLSRRHSKAILQAGGLADCLLYLEFFSINAQRNALAIAANCCQSITPDEFHFVADSLPLLTQRLTHQDKKSVESTCLCFARLVDNFQHEENLLQQVASKDLLTNVQQLLVVTPPILSSGMFIMVVRMFSLMCSNCPTLAVQLMKQNIAETLHFLLCGASNGSCQEQIDLVPRSPQELYELTSLICELMPCLPKEGIFAVDTMLKKGNAQNTDGAIWQWRDDRGLWHPYNRIDSRIIEAAHQVGEDEISLSTLGRVYTIDFNSMQQINEDTGTARAIQRKPNPLANTNTSGYSELKKDDARAQLMKEDPELAKSFIKTLFGVLYEVYSSSAGPAVRHKCLRAILRIIYFADAELLKDVLKNHAVSSHIASMLSSQDLKIVVGALQMAEILMQKLPDIFSVYFRREGVMHQVKHLAESESLLTSPPKACTNGSGSLGSTTSVSSGTATAATNAAADLGSPSLQHSRDDSLDLSPQGRLSDVLKRKRLPKRGPRRPKYSPPRDDDKVDNQAKSPTTTQSPKSSFLASLNPKTWGRLSAQSNSNNIEPARTAGVSGLARAASKDTISNNREKIKGWIKEQAHKFVERYFSSENMDGSNPALNVLQRLCAATEQLNLQVDGGAECLVEIRSIVSESDVSSFEIQHSGFVKQLLLYLTSKSEKDAVSREIRLKRFLHVFFSSPLPGEEPIGRVEPVGNAPLLALVHKMNNCLSQMEQFPVKVHDFPSGNGTGGSFSLNRGSQALKFFNTHQLKCQLQRHPDCANVKQWKGGPVKIDPLALVQAIERYLVVRGYGRVREDDEDSDDDGSDEEIDESLAAQFLNSGNVRHRLQFYIGEHLLPYNMTVYQAVRQFSIQAEDERESTDDESNPLGRAGIWTKTHTIWYKPVREDEESNKDCVGGKRGRAQTAPTKTSPRNAKKHDELWHDGVCPSVSNPLEVYLISSPPENITFEDPSLDVILLLRVLHAISRYWYYLYDNAMCKEIIPTSEFINSKLTAKANRQLQDPLVIMTGNIPTWLTELGKTCPFFFPFDTRQMLFYVTAFDRDRAMQRLLDTNPEINQSDSQDSRVAPRLDRKKRTVNREELLKQAESVMQDLGSSRAMLEIQYENEVGTGLGPTLEFYALVSQELQRADLGLWRGEEVTLSNPKGSQEGTKYIQNLQGLFALPFGRTAKPAHIAKVKMKFRFLGKLMAKAIMDFRLVDLPLGLPFYKWMLRQETSLTSHDLFDIDPVVARSVYHLEDIVRQKKRLEQDKSQTKESLQYALETLTMNGCSVEDLGLDFTLPGFPNIELKKGGKDIPVTIHNLEEYLRLVIFWALNEGVSRQFDSFRDGFESVFPLSHLQYFYPEELDQLLCGSKADTWDAKTLMECCRPDHGYTHDSRAVKFLFEILSSFDNEQQRLFLQFVTGSPRLPVGGFRSLNPPLTIVRKTFESTENPDDFLPSVMTCVNYLKLPDYSSIDIMREKLLIAAREGQQSFHLS

Target ORF information:

RefSeq Version	XM_005635809.2
Organism	Canis lupus familiaris(Dog)
Definition	Canis lupus familiaris thyroid hormone receptor interactor 12 (TRIP12), transcript variant X9, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005635809.2

ORF Insert Sequence:

ATGTCCAACC GGCCTAATAA CAATCCAGGG GGGTCACTGC GACGTTCACA GAGGAACACT 
GCCGGGGCCC AACCACAAGA CGACTCGATA GGAGGAAGAA GCTGCAGTTC ATCATCTGCT 
GTCATAGTTC CACAACCAGA GGATCCAGAC AGAGCCAATA CTTCAGAAAG GCAAAAAACG 
GGGCAGGTGC CTAAGAAAGA CAATTCTCGA GGAGTGAAAC GCAGTGCTAG TCCAGACTAC 
AACAGGACCA ATTCTCCTAG CTCTGCCAAA AAACCAAAAG CACTTCAGCA TACTGAATCT 
CCCTCAGAAA CAAATAAGCC ACATAGTAAG TCGAAGAAGA GACATTTAGA CCAGGAGCAA 
CAACTGAAAT CTGCACAGTC GCCATCAACA AGTAAGGCTC ACACCAGAAA GAGTGGGGCC 
ACCGGCGGCT CACGGAGTCA GAAAAGAAAG AGGACAGAGA GTTCTTGTCT AAAGAGTGGT 
TCTGTGTCTG AGTCAACTGG TGCCGAAGAG AGATCTGCAA AACCCACCAA GCTGGCTTCA 
AAATCCGCCG CCTCGGCCAA AGCTGGGTGC AGCACCATCA CTGATTCTTC CTCCGCTGCC 
TCTACTTCCT CCTCATCTTC TGCTGTCGCC TCGGCCTCCT CCACTGTACC ACCAGGGGCC 
AGGGTGAAAC AAGGAAAAGA CCAGAGCAAG GCCAGGCGTT CCCGTTCAGC ATCCAGTCCC 
AGTCCCAGAA GAAGTAGCAG GGAAAAGGAA CAGAGTAAAA CTGGTGGCTC TTCAAAATTC 
GATTGGGCTG CTCGTTTCAG CCCTAAAGTT AGCCTTCCTA AAACCAAACT GTCTCTTCCA 
GGGTCTTCTA AGTCAGAGAC ATCAAAACCT GGACCTTCTG GATTACAGGC CAAATTAGCA 
AGTTTAAGAA AATCTACCAA GAAGCGCAGT GAATCCCCAC CTGCTGAGCT CCCCAGTTTG 
AGGCGGAGCA CACGCCAAAA GACCACGGGC TCCTGTGCTA GTGCCAGTCG GCGAGGCTCT 
GGCCTGGGCA AAAGAGGAGC AGCTGAAGCT CGTCGACAGG AGAAGATGGC GGACCCTGAA 
AGCAGCCAGG AGACCGTGAA TTCTTCAGCT GCACGGACAG ACGAAACTCC CCAAGGAGCT 
GCAGCCTCTA GTTCTGTTGC AGGGGCTGTG GGCATGACCA CCTCTGGGGA GAGTGAATCC 
GATGATTCTG AGATGGGACG ACTACAAGCT TTGTTAGAGG CCAGGGGTCT TCCTCCTCAT 
CTGTTTGGCC CTCTCGGTCC TCGGATGTCA CAACTTTTCC ATAGAACAAT TGGAAGCGGA 
GCTAGCTCTA AGGCCCAGCA GCTTCTGCAA GGACTACAAG CAAGTGATGA AAGCCAGCAA 
CTCCAGGCAG TTATTGAAAT GTGTCAGTTA TTGGTTATGG GAAATGAGGA GACGCTGGGA 
GGGTTTCCGG TCAAGAGTGT CGTTCCAGCT TTGATAACAT TACTGCAGAT GGAGCATAAT 
TTCGATATTA TGAACCATGC TTGTCGAGCC TTAACATATA TGATGGAAGC ACTTCCTCGG 
TCATCTGCTG TTGTAGTAGA TGCAATTCCT GTCTTTTTAG AAAAGCTGCA AGTTATTCAG 
TGTATTGATG TCGCAGAGCA GGCCCTGACT GCCTTGGAGA TGTTATCACG CAGACACAGT 
AAAGCCATTC TACAGGCGGG TGGTTTGGCA GACTGCTTGC TGTATCTAGA ATTCTTCAGC 
ATAAATGCCC AAAGAAATGC ACTAGCAATT GCAGCCAATT GCTGCCAGAG TATCACGCCA 
GATGAATTTC ATTTTGTGGC AGATTCCCTC CCGTTGCTTA CCCAAAGGCT AACCCATCAG 
GACAAAAAGT CAGTTGAAAG CACTTGCCTT TGTTTTGCAC GTCTAGTGGA CAACTTCCAA 
CATGAGGAGA ATTTACTCCA GCAGGTTGCC TCCAAAGATC TGCTTACGAA TGTTCAACAA 
CTCTTGGTAG TGACTCCACC CATTTTAAGT TCTGGGATGT TTATAATGGT GGTTCGCATG 
TTTTCTCTGA TGTGTTCCAA CTGTCCAACT TTAGCTGTTC AACTTATGAA GCAAAACATT 
GCAGAAACAC TTCACTTTCT CCTGTGTGGT GCCTCTAATG GAAGTTGTCA GGAACAGATT 
GATCTTGTTC CACGAAGTCC ACAAGAACTG TATGAACTGA CATCTCTGAT TTGCGAACTT 
ATGCCATGTT TACCAAAAGA AGGCATTTTT GCAGTTGATA CCATGCTGAA GAAGGGAAAT 
GCGCAGAACA CAGACGGTGC GATTTGGCAG TGGCGTGATG ACCGTGGCCT TTGGCATCCC 
TATAACAGGA TTGACAGCCG GATCATTGAG GCAGCCCATC AGGTCGGTGA GGATGAGATA 
AGCTTGTCCA CTCTGGGACG AGTTTATACT ATTGATTTTA ATTCTATGCA GCAAATCAAT 
GAGGACACGG GAACAGCACG TGCCATTCAG AGAAAACCTA ACCCCTTAGC CAATACTAAC 
ACTAGTGGAT ATTCAGAGTT AAAGAAGGAT GATGCTCGAG CACAGCTTAT GAAAGAGGAT 
CCGGAACTGG CTAAGTCTTT TATTAAGACA TTATTTGGTG TTCTTTATGA AGTGTATAGT 
TCCTCAGCAG GACCTGCGGT CAGACATAAG TGCCTTAGAG CAATTCTTAG GATAATTTAT 
TTTGCGGATG CTGAACTTCT GAAGGATGTC CTGAAAAATC ATGCTGTTTC AAGTCACATT 
GCTTCCATGC TGTCAAGCCA AGACCTGAAG ATAGTAGTTG GAGCACTTCA GATGGCAGAA 
ATCTTAATGC AGAAGTTACC TGATATTTTT AGTGTTTACT TCAGAAGAGA AGGTGTAATG 
CATCAAGTAA AACACTTAGC AGAATCAGAG TCTTTGTTGA CAAGTCCACC AAAGGCATGT 
ACGAATGGGT CAGGATCCCT GGGATCCACG ACGTCAGTCA GCAGTGGAAC AGCCACGGCC 
GCCACCAATG CTGCAGCTGA CCTGGGGTCC CCCAGCTTGC AGCACAGCAG AGATGACTCT 
TTAGATCTGA GCCCTCAAGG TCGATTAAGT GATGTTCTAA AGAGAAAACG GCTGCCAAAA 
CGAGGGCCAA GAAGGCCAAA GTATTCACCT CCAAGAGATG ATGACAAAGT AGACAATCAA 
GCTAAAAGCC CTACCACTAC TCAGTCACCT AAATCTTCTT TCCTGGCAAG CTTGAATCCA 
AAAACGTGGG GAAGGTTAAG CGCACAGTCC AACAGCAACA ACATCGAACC AGCACGAACT 
GCGGGAGTCA GTGGTCTTGC CAGGGCCGCC TCCAAGGACA CCATATCCAA TAACAGAGAA 
AAAATTAAAG GTTGGATTAA GGAACAGGCG CATAAATTTG TAGAGCGTTA TTTCAGTTCT 
GAGAACATGG ATGGAAGCAA CCCTGCACTG AATGTCCTTC AGAGACTTTG TGCTGCAACT 
GAACAACTCA ACCTCCAGGT GGATGGTGGA GCTGAGTGCC TTGTAGAAAT CCGTAGCATA 
GTCTCAGAGT CTGATGTTTC ATCATTTGAA ATCCAACATA GTGGATTTGT GAAGCAGCTG 
TTACTTTATT TGACATCTAA AAGTGAAAAG GATGCTGTGA GCCGAGAGAT CAGATTAAAG 
AGATTCCTTC ACGTATTTTT TTCTTCACCA CTTCCTGGAG AAGAGCCCAT TGGAAGAGTA 
GAACCAGTGG GTAATGCGCC TTTGTTGGCA TTAGTTCACA AGATGAACAA CTGCCTCAGC 
CAGATGGAAC AGTTTCCAGT CAAAGTACAT GACTTCCCCA GTGGGAACGG GACAGGAGGC 
AGCTTTTCTC TCAACAGAGG ATCACAGGCT TTAAAATTTT TCAACACACA CCAGTTAAAA 
TGCCAGTTAC AAAGACATCC AGACTGTGCA AATGTGAAGC AGTGGAAGGG CGGGCCGGTC 
AAGATTGATC CCCTGGCTTT GGTACAAGCT ATTGAGAGAT ACCTTGTAGT TAGAGGGTAT 
GGAAGAGTAA GAGAAGATGA TGAAGACAGT GATGATGATG GCTCTGATGA GGAAATAGAC 
GAGTCTCTGG CCGCTCAGTT CTTAAATTCC GGAAACGTAA GGCACAGGCT ACAGTTTTAT 
ATTGGAGAAC ATTTGCTACC ATATAACATG ACTGTATATC AGGCAGTCCG GCAGTTCAGT 
ATACAAGCTG AGGATGAGCG GGAGTCCACA GATGATGAGA GCAACCCTCT GGGAAGAGCT 
GGTATTTGGA CAAAGACTCA TACAATATGG TACAAACCTG TGAGAGAGGA TGAAGAAAGT 
AATAAAGATT GTGTTGGTGG TAAAAGAGGA AGAGCCCAAA CGGCTCCAAC AAAAACATCC 
CCTAGAAATG CAAAAAAGCA TGATGAGTTA TGGCATGATG GAGTATGCCC ATCAGTATCC 
AATCCTTTAG AAGTTTACCT CATCTCCTCG CCACCTGAGA ATATAACTTT TGAAGACCCG 
TCCTTAGATG TGATTCTTCT TCTAAGAGTT TTACATGCCA TCAGCCGATA CTGGTATTAC 
TTGTATGATA ATGCAATGTG CAAGGAGATT ATTCCAACTA GTGAATTTAT TAACAGTAAG 
TTGACAGCAA AAGCAAATAG GCAGCTTCAA GATCCTTTAG TAATCATGAC AGGAAATATC 
CCAACGTGGC TTACTGAACT AGGAAAAACC TGCCCATTTT TCTTCCCTTT TGATACCCGG 
CAAATGCTTT TTTACGTAAC TGCATTTGAT CGGGACCGAG CAATGCAAAG ATTACTTGAT 
ACCAACCCAG AAATCAACCA GTCGGATTCT CAAGATAGCA GAGTTGCACC TAGATTGGAT 
AGAAAAAAAC GTACTGTGAA CAGAGAGGAG CTGCTGAAAC AAGCGGAGTC TGTAATGCAG 
GATCTCGGTA GCTCACGGGC CATGTTAGAA ATCCAGTATG AAAATGAGGT TGGTACAGGT 
CTTGGGCCTA CACTGGAGTT TTATGCACTT GTATCTCAGG AACTTCAGAG AGCTGACTTG 
GGTCTCTGGA GAGGTGAAGA AGTGACTCTC AGCAATCCAA AAGGAAGCCA AGAAGGGACC 
AAGTATATTC AAAACCTCCA GGGCCTGTTT GCACTTCCCT TTGGTAGGAC AGCTAAGCCA 
GCCCATATCG CAAAGGTTAA AATGAAGTTT CGCTTCTTAG GAAAATTAAT GGCCAAGGCT 
ATCATGGATT TCAGATTGGT GGACCTTCCC CTTGGTTTAC CCTTTTATAA GTGGATGCTA 
AGGCAAGAAA CTTCACTGAC GTCGCATGAT TTGTTTGACA TTGACCCAGT TGTAGCCAGA 
TCAGTGTATC ACCTAGAAGA CATTGTCAGA CAGAAGAAAA GACTTGAGCA AGATAAATCC 
CAGACCAAAG AGAGTCTACA ATATGCATTA GAAACCCTGA CTATGAATGG CTGCTCAGTT 
GAAGATCTAG GATTGGATTT CACGCTGCCA GGGTTTCCCA ACATTGAACT GAAGAAAGGA 
GGAAAGGATA TACCAGTTAC CATCCACAAT TTAGAGGAGT ATCTAAGACT GGTTATATTC 
TGGGCACTAA ATGAAGGCGT TTCCAGGCAA TTTGATTCCT TCAGAGATGG ATTTGAATCT 
GTCTTCCCAC TTAGTCATCT TCAGTACTTC TACCCGGAAG AACTGGATCA GCTCTTGTGC 
GGCAGTAAAG CAGACACGTG GGATGCGAAG ACACTGATGG AGTGCTGCAG GCCGGACCAC 
GGTTATACTC ATGACAGTCG GGCTGTGAAG TTCTTGTTTG AGATTCTCAG TAGTTTTGAT 
AATGAGCAGC AGAGGTTGTT TCTCCAGTTT GTGACAGGTA GCCCAAGATT GCCTGTTGGA 
GGCTTCCGGA GTCTGAATCC ACCTCTGACA ATTGTCCGGA AGACCTTCGA GTCCACGGAG 
AACCCCGACG ACTTCTTGCC CTCTGTAATG ACTTGTGTGA ACTACCTTAA GTTGCCGGAC 
TACTCGAGCA TTGACATCAT GCGTGAGAAA CTGTTGATTG CAGCGAGAGA AGGGCAGCAG 
TCATTCCATC TTTCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Gene Symbol	TRIP12
Entrez Gene ID	477400
Full Name	thyroid hormone receptor interactor 12
General protein information	Preferred Names thyroid hormone receptor interactor 12 Names probable E3 ubiquitin-protein ligase TRIP12 E3 ubiquitin-protein ligase TRIP12
Gene Type	protein-coding
Organism	Canis lupus familiaris(dog)

Anopheles gambiae (African malaria mosquito)	AgaP_AGAP001296	XP_321851.5
	TRIP12	XP_002799130.1
Mus musculus (house mouse)	Trip12	NP_598736.4
Xenopus tropicalis (tropical clawed frog)	trip12	NP_001136384.1
Homo sapiens (human)	TRIP12	NP_004229.1
Canis lupus familiaris (dog)	TRIP12	XP_005635860.1
Gallus gallus (chicken)	TRIP12	XP_004937121.1
Pan troglodytes (chimpanzee)	TRIP12	XP_001138950.1
Bos taurus (cattle)	TRIP12	NP_001178132.1
Arabidopsis thaliana (thale cress)	KAK	NP_849567.2
Rattus norvegicus (Norway rat)	Trip12	NP_001026829.1
Danio rerio (zebrafish)	trip12	XP_005159284.1
Oryza sativa (rice)	Os02g0101700	NP_001045591.1

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
30% OFF

TRIP12 cDNA ORF clone, Canis lupus familiaris(dog)

Gene

Clones

gRNAs

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