IL-2 R alpha/CD25, His & Avi, Human
IL-2 R Alpha/CD25, His & Avi, Human

Immobilized IL-2 R alpha/CD25, His & Avi, Human, His Tag at 0.5 μg/ml (100 μL/well) on the plate. Dose response curve for Anti-IL-2 R alpha Antibody, hFc Tag with the EC50 of 5.5 ng/ml determined by ELISA.

IL-2 R Alpha/CD25, His & Avi, Human

The purity of IL-2 R alpha/CD25, His & Avi, Human is greater than 95% as determined by SEC-HPLC.

IL-2 R Alpha/CD25, His & Avi, Human

IL-2 R alpha/CD25, His & Avi, Human on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.

IL-2 R Alpha/CD25, His & Avi, Human

Immobilized IL-2 R alpha/CD25, His & Avi, Human, His Tag at 0.5 μg/ml (100 μl/well) on the plate. Dose response curve for Anti-IL-2 R alpha Antibody, hFc Tag with the EC50 of 5.3 ng/ml determined by ELISA.

IL-2 R Alpha/CD25, His & Avi, Human

IL-2 R alpha/CD25, His & Avi, Human, His Tag captured on CM5 Chip via anti-his antibody can bind Human IL-2, No Tag with an affinity constant of 16.82 nM as determined in SPR assay (Biacore T200).

IL-2 R alpha/CD25, His & Avi, Human

Human IL-2 receptor alpha (IL-2 R alpha), also known as Tac antigen and as CD25, was initially identified as a 55 kDa membrane glycoprotein that is capable of binding IL‑2.eceptor for interleukin-2. The receptor is involved in the regulation of immune tolerance by controlling regulatory T cells (TREGs) activity. TREGs suppress the activation and expansion of autoreactive T-cells.
Z04273
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Product Introduction
Species Human
Protein Construction
IL-2 R alpha/CD25 (Glu22-Cys213)
Accession # P01589		 His Avi
N-term C-term
Purity > 95% as determined by Bis­Tris PAGE
Endotoxin Level Less than 1EU per μg by the LAL method.
Biological Activity Measured by its binding ability in a functional ELISA.Test result was comparable to standard batch.
Expression System HEK293
Theoretical Molecular Weight 24.6 kDa
Apparent Molecular Weight Due to glycosylation, the protein migrates to 40-50 kDa based on Bis-Tris PAGE result.
Formulation Lyophilized from 0.22μm filtered solution in PBS (pH 7.4).
Reconstitution Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water.
Storage & Stability Upon receiving, the product remains stable for 6 months at -20℃ or below. Upon reconstitution, the product should be stable for 3 months at -80℃. Avoid repeated freeze-thaw cycles.

Examples
  • IL-2 R Alpha/CD25, His & Avi, Human
    • IL-2 R Alpha/CD25, His & Avi, Human

    Immobilized IL-2 R alpha/CD25, His & Avi, Human, His Tag at 0.5 μg/ml (100 μL/well) on the plate. Dose response curve for Anti-IL-2 R alpha Antibody, hFc Tag with the EC50 of 5.5 ng/ml determined by ELISA.

  • IL-2 R Alpha/CD25, His & Avi, Human
    • IL-2 R Alpha/CD25, His & Avi, Human

    The purity of IL-2 R alpha/CD25, His & Avi, Human is greater than 95% as determined by SEC-HPLC.

  • IL-2 R Alpha/CD25, His & Avi, Human
    • IL-2 R Alpha/CD25, His & Avi, Human

    IL-2 R alpha/CD25, His & Avi, Human on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.

  • IL-2 R Alpha/CD25, His & Avi, Human
    • IL-2 R Alpha/CD25, His & Avi, Human

    Immobilized IL-2 R alpha/CD25, His & Avi, Human, His Tag at 0.5 μg/ml (100 μl/well) on the plate. Dose response curve for Anti-IL-2 R alpha Antibody, hFc Tag with the EC50 of 5.3 ng/ml determined by ELISA.

  • IL-2 R Alpha/CD25, His & Avi, Human
    • IL-2 R Alpha/CD25, His & Avi, Human

    IL-2 R alpha/CD25, His & Avi, Human, His Tag captured on CM5 Chip via anti-his antibody can bind Human IL-2, No Tag with an affinity constant of 16.82 nM as determined in SPR assay (Biacore T200).


Background
Target Background Human IL-2 receptor alpha (IL-2 R alpha), also known as Tac antigen and as CD25, was initially identified as a 55 kDa membrane glycoprotein that is capable of binding IL‑2.eceptor for interleukin-2. The receptor is involved in the regulation of immune tolerance by controlling regulatory T cells (TREGs) activity. TREGs suppress the activation and expansion of autoreactive T-cells.
Synonyms CD25; IDDM10; IL-2 R alpha; IL2R; IL2RA; p55; TCGFR

For research use only. Not intended for human or animal clinical trials, therapeutic or diagnostic use.