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ウサギ・モノクローナル抗体作製サービス

MonoRab<sup>®</sup> Rabbit monoclonal antibody generation Services

ウサギ・モノクローナル抗体(MonoRab^®)は、一般的なマウス・モノクローナル抗体より、親和性と特異性が高いというメリットがあります。ほとんどのマウスモノクローナル抗体のKD値はナノモルやサブナノモルレベルであるのに対して、MonoRab^®のKD値は通常ピコモルレベルとなります。また、ウサギ免疫系は抗原認識能が多様で、非常によく似たタンパク質や配列の違いを認識することができます。加えて、広い範囲でのエピトープ、新規エピトープ、小さなエピトープも認識できます。特にマウスにおいて免疫原性がない抗原であっても認識することが可能です。したがって、MonoRab^®は抗イディオタイプ抗体の開発、免疫組織化学染色用抗体の作製やその他多くの用途に利用できます。

GenScriptは独自のMonoRab^®テクノロジーを利用して、お客様の要件を満たす高品質のカスタムMonoRab^®を提供いたします。

Want to Learn the Benefits of Rabbit mAbs?

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MonoRab^® is a full scale service including antigen preparation, rabbit immunization, recombinant antibody production, and antibody sequencing; ensuring ultimate levels of reproducibility and mAb purification. Talk to our technical specialists today to learn why GenScript's MonoRab^® service is perfect for everything from basic research,to in vitro diagnostics (IVD), anti-idiotype (Anti-ID) mAb generation, and more. Get a quote to let GenScript help make your research easy today!

Exceptional Specificity & Sensitivity

Picomolar K_D levels Elicits an immune response not
immunogenic in mice
Extremely Fast Turn Around Time

From antigen sequence to purified mAb in as little as 4 Months
Cuts the production time in half
Most Cost Effective Pricing

Highly competitive price
No extra licensing or royalty fees

Workflow

Milestone	Details	Times (weeks)
Immunogen preparation	Antigen production KLH conjugation for peptide Ag	3
Animal Immunization	New Zealand White Rabbit 2 Animals/group # groups optional	10-12
Cell Fusion, Screening, Subcloning & Ab Sequencing	1 Electro fusion of up to 150 96- well plates ELISA supernatant screening Subcloning V-region sequencing of up to 10 monoclones Up to 10 parental clones selected for customer analysis Up to 5 clones selected by Client	10-11
FREE Epitope Binning	Free epitope binning service included in every MonoRab^® purchase Service includes epitope binning on 5 monoRab™ chosen after in house screening	0.5
Optional Recombinant Ab Production	Up to 5 clones selected by client Ab expression and purification from plasmid QC with ELISA, SDS-PAGE, & OD280nm	4-5

QC/Validation

Included QC/Validation
High Throughout ELISA
High Throughput FACS Analysis
Epitope Binning
SDS-PAGE
OD280 nm

Deliverables

Milestone	Deliverables
Immunogen preparation	Isolated protein or peptide
Animal Immunization	Test bleed report OR 5 mL sample after 3^rd boost
Cell Fusion, Screening, Subcloning & Ab Sequencing	Up to 10 supernatants sent to customer for in house analysis mAb sequencing report for up to 5 clones with supernatant
FREE Epitope Binning	Epitope binning report
Recombinant Ab Production	1 mg purified Ab for each clone

Mid-Project Decisions

Milestone	Mid-Project Options
Immunogen preparation
Animal Immunization	Modify immunization strategy Proceed with fusion
Cell Fusion, Screening, Subcloning & Ab Sequencing	Alter screening Select up to 5 monoclones for sequencing Continue with other services: Ab screening, Ab production, ect
FREE Epitope Binning
Recombinant Ab Production

Applications

Application	Description
Exceptional Immunization with Difficult Antigens	Nanobodies Small molecules Extremely similar epitopes Non-immunogenic in mice Phosphorylated antigens
Perfect for use in In Vitro Diagnostics (IVD)	Immuno-detection of drug compounds in serum or other biological samples Immunoassay development for therapeutic antibody development
Incomparable Anti-Idiotype (Anti-ID) mAb Generation	Ab targeting the Ag binding site of another Ab Tracking Ab drug in serum or biological fluid Powerful for Ab drug PK, PD, and immunogenicity studies

Application Tailored MonoRab^® Services

Service	Description
Anti-Idiotype Antibody Development	Detect Free and/or Bound Antibody Drug Ideal for Pharmacokinetic or Immunogenicity Studies Proven Track Record of High Affinity Anti-Idiotype mAbs Guaranteed Anti-ID Pair Development Stable & Easy Scale Up
Single Chain Variable Fragment Generation	Generate Highly Specific ScFvs Perfect for In-Vivo Diagnostics, Drug Targeting, and/or Therapeutics Multiple Delivery Options to Accommodate Any Application Cell Line and DNA Based Immunization for Natural ScFv Generation ELISA and FACS Based Screening Methods
Immunohistochemistry Specific mAb Generation	Screen Up to 30 Supernatants for Your Perfect Binder Exceptional Specificity to Eliminate Background Highly Sensitive to Difficult Targets Pre-Hybridoma IHC Analysis at GenScript Ideal for Tissue Based In-Vitro Diagnostics

Case Studies

A The cross reactivity rate of GenScript produced anti-Ractopamine (small molecule) rabbit mAb is 0.0304%, showing low cross reactivity rates with similar compounds.
B GenScript produced anti-idiotype rabbit mAb 90G12F8 binds with Pembrolizumab (mAb drug) with an EC50 of 5.001 ng/ml as shown via ELISA.
C Genscript produced N-Terminal anti-flag-tag rabbit (section 1) mAb shows higher specificity and affinity than those produced in mouse (section 2) as shown via western blot.
D GenScript produced anti-idiotype rabbit mAb 90G12F8 has a K_D valu e of 2.3x10-12 M against the antibody drug (Pembrolizumab) as shown via ELISA.

ウサギ・モノクローナル抗体作製サービス

Want to Learn the Benefits of Rabbit mAbs?

Exceptional Specificity & Sensitivity

Extremely Fast Turn Around Time

Most Cost Effective Pricing

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B

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GenScript's Premier Rabbit Monoclonal Antibody Generation Platform, Featured in this Week's "Supplier Insider" Video from "The Scientist".

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