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モノクローナル抗体とは違い、ポリクローナル抗体は抗原分子上に存在する多数の抗原決定基に対する抗体が混在しています。ポリクローナル抗体は免疫アッセイをする際、一次抗体または二次抗体として使われます。弊社がAAALAC Internationalで認証した動物施設を持つ、マウス、ラット、ウサギ、ニワトリ、ヤギ/羊、SPFの動物および他の種由来のポリクローナル抗体のサービスを提供しています。弊社のポリクローナル抗体パッケージには、抗原の設計、ペプチドの合成、免疫、精製、エリサテストおよび凍結乾燥サービスを含まれています。お客様は、簡単に自分の必要なパッケージを選択することができます。GenScriptは品質保証する抗体を提供します。
保証:
当社独自のOptimumAntigenTM設計ツール、T- MaxTMアジュバントおよびナノテクノロジーにより、弊社がデザイン、合成、コンジュゲイションしたペプチドを抗原としてウサギでポリクローナル抗体を作製する場合、我々は製品の最終ELISA力価が1:32,000またはそれ以上であることを保証します。すべてのタンパク質抗原（3 mg,>85% 純度）について、弊社は陽性Western blot結果及び任意のホストにつき、ELISA力価が1:32,000またはそれ以上であることを保証します。保証が満たさない場合は、プロジェクトは無料で再実施します。
サービス内容:
GenScriptは、ポリクローナル抗体作製の様々なパッケージを提供しています。当社の経験豊富な専門者は、お客様に最適なサービスパッケージを案内します。
1. 一番人気: Complete Affinity-Purified Peptide Polyclonal Antibody Package (Rabbit) (Cat.No.: SC1031)
パッケージ内容: |
納品 (納期 15-17 週間): |
- Peptide synthesis
- Two rabbits, pre-immune bleed, 4
immunizations and final bleed
- Peptide affinity purification
- ELISA test
- Lyophilization
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- 3-50 mg affinity-purified antibodies (>1:32,000) (lyophilized)
- 5 mg of unconjugated peptide
- 1-2 ml of pre-immune serum*
- ELISA results, MS and HPLC reports
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2. Western-GUARANTEED Peptide Polyclonal Antibody Package (Rabbit) (Cat.No.: SC1390)
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パッケージ内容:
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納品 in (18-20 weeks): |
- Peptide design and synthesis
- Peptide-KLH conjugation
- 6 rabbits immunization, Pre-immune bleeds and test bleeds
- Affinity purification and ELISA results
- Western blot test
- Lyophilization
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- 3-50 mg purified antibodies (lyophilized)
- 1-2 ml of pre-immune serum*
- 5 mg unconjugated peptide
- MS and HPLC reports
- Western blot, ELISA titer reports
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3. Phospho-Specific Polyclonal Antibody Package (Rabbit) (Cat.No.: SC1044)
パッケージ内容: |
納品 (in 15-17 weeks): |
- Synthesis of two peptides, one with modification (10-15 a.a, >90% purity, 15 mg)and the other without modification (10-15 a.a, >90% purity, 10 mg)
- A cysteine is automatically added to the C terminus (N terminus) of the peptide and conjugated to KLH.
- Two rabbits, pre-immune bleed, four immunizations/rabbit
- Prepare two affinity columns
- Antibody purification by modified-peptide affinity column and unmodified-peptide cross-adsorption.
- ELISA test
- Lyophilization
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- 1-30 mg phospho-specific antibodies (lyophilized) from two rabbits
- 5 mg of unconjugated phospho peptides and 5 mg of unconjugated non-phospho peptides
- 1-2 ml of pre-immune serum*
- ELISA results, MS and HPLC reports
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4. Express Complete Affinity-Purified Peptide Polyclonal Antibody Package (Rabbit)(Cat.No.: SC1180)
パッケージ内容: | 納品 (in 15-17 weeks): |
- Peptide synthesis
- Cysteine addition (C' or N')
- Conjugation to KLH
- Four rabbits, pre-immune bleed
- Three immunizations/rabbit
- Final bleed
- 5 mg peptide conjugated to resin
- Affinity purified antibody
- ELISA test
- Lyophilization (optional)
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- 4-50 mg affinity-purified antibodies (lyophilized or liquid) from 2 best rabbits
- 2 ml of pre-immune serum
- 5 mg unconjugated peptide
- ELISA results, MS and HPLC reports
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* For collection and purification of antibodies from separated animals, a total of 2 ml of pre-immune serum (1 ml from each of the 2 animals) are provided. For antibody purification from pooled serum, 1 ml of pre-immune serum is provided.
5. Or choose from other convenient packages designed to meet all your needs
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Polyclonal Antibody Package List
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Species |
Cat.No. |
Package Name
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| Mouse |
SC1046 |
Complete Peptide Polyclonal Antibody Package |
| SC1049 |
Complete Protein A-Purified Peptide Polyclonal Antibody Package |
| Rabbit |
SC1015 |
Complete Peptide Polyclonal Antibody Package |
| SC1045 |
Complete Protein A-Purified Peptide Polyclonal Antibody Package |
| SC1031 |
Complete Affinity-Purified Peptide Polyclonal Antibody Package |
| SC1044 |
Phospho-Specific Polyclonal Antibody Package |
| Goat |
SC1050 |
Complete Peptide Polyclonal Antibody Package |
| SC1051 |
Complete Protein G-Purified Peptide Polyclonal Antibody Package |
| SC1368 |
Complete Affinity-purified Peptide Polyclonal Antibody Package |
| Chicken |
SC1055 |
Complete Peptide Polyclonal IgY Antibody Package |
| SC1056 |
Complete Affinity-Purified Peptide Polyclonal Antibody Package |
| Rat |
SC1313 |
Complete Peptide Polyclonal Antibody Package |
| SC1314 |
Complete Protein G-Purified Peptide Polyclonal Antibody Package |
Note: Lyophilization extends the shelf life of your antibodies, eliminates leaking problems and loosens up the stringent temperature requirement during shipping, and reduces the overall weight of antibodies to be shipped.
Deliverables
Our typical delivery includes purified antibodies, pre-immune serum, unconjugated peptide, ELISA test report, MS and HPLC results.
| Purification |
Cat. No. |
Animal
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Deliverables
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| Pre-immune Serum |
Antibody |
Peptide |
Antisera |
| N/A |
SC1015 |
2 rabbits |
1-2 ml |
– |
5 mg |
100-120 ml |
| SC1050 |
1 goat |
2 ml |
– |
5 mg |
≈1,000 ml |
| SC1046 |
5 mice |
50-100 µl |
– |
5 mg |
2.5 ml |
| SC1313 |
3 rats |
50-100 µl |
– |
5 mg |
5 ml |
| Affinity |
SC1031 |
2 rabbits |
1-2 ml |
3-50 mg |
5 mg |
– |
| SC1368 |
1 goat |
2 ml |
3-5 mg |
5 mg |
≈900 ml |
| SC1056 |
2 chickens |
1-2 ml |
Quote |
5 mg |
4 ml |
| Protein A |
SC1049 |
5 mice |
50-100 µl |
2.5-12.5 mg |
5 mg |
– |
| SC1045 |
2 rabbits |
1-2 ml |
200-500 mg |
5 mg |
– |
| Protein G |
SC1051 |
1 goat |
2 ml |
300-1,000 mg |
5 mg |
≈900 ml |
| SC1314 |
3 rats |
50-100 μl |
4.5-22.5 mg |
5 mg |
– |
| IgY |
SC1055 |
2 chickens |
1-2 ml |
Quote |
5 mg |
4 ml |
お見積およびご注文:
 お見積およびご質問についてのお問い合わせは電話、電子メール、ファックスまたは弊社のsecured オンライン見積システムで受け付けております。
弊社は、月曜日から金曜日までの営業日においては24時間サービスを提供しています。
いつでもお客様に対応します。
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