Hgs cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene
Clones
gRNAs

Summary

Gene Symbol	Hgs
Entrez Gene ID	56084
Full Name	hepatocyte growth factor-regulated tyrosine kinase substrate
Synonyms	Hrs
General protein information	Preferred Names hepatocyte growth factor-regulated tyrosine kinase substrate Names hepatocyte growth factor-regulated tyrosine kinase substrate HGF-regulated tyrosine kinase substrate SNAP-25-interacting protein Hrs-2
Gene Type	protein-coding
Organism	Rattus norvegicus(Norway rat)
Genome	Chromosome: 10 Map Location: 10q32.3
Summary	interacts with SNAP-25 and is involved in regulation of neurosecretion [RGD, Feb 2006].

mRNA and Protein(s)

mRNA	Protein	Name
XM_006247908.3	XP_006247970.1	hepatocyte growth factor-regulated tyrosine kinase substrate isoform X1
NM_019387.2	NP_062260.2	hepatocyte growth factor-regulated tyrosine kinase substrate
XM_006247909.3	XP_006247971.1	hepatocyte growth factor-regulated tyrosine kinase substrate isoform X2

Pathways from BioSystems

Homologies

	HGS	XP_001111673.1
Bos taurus (cattle)	HGS	NP_001039554.1
Danio rerio (zebrafish)	hgs	NP_956162.1
Caenorhabditis elegans (roundworm)	hgrs-1	NP_501375.2
Canis lupus familiaris (dog)	HGS	XP_005624060.1
Mus musculus (house mouse)	Hgs	NP_001152800.1
Gallus gallus (chicken)	HGS	XP_426233.3
Drosophila melanogaster (fruit fly)	Hrs	NP_525099.3
Pan troglodytes (chimpanzee)	HGS	XP_511742.4
Rattus norvegicus (Norway rat)	Hgs	NP_062260.2
Xenopus tropicalis (tropical clawed frog)	hgs	NP_001016513.1
Homo sapiens (human)	HGS	NP_004703.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP008887	XP_319634.4

Gene Ontology
Bibliography

Related articles in PubMed

The ubiquitin ligase deltex-3l regulates endosomal sorting of the G protein-coupled receptor CXCR4.
Holleman J, Marchese A
Molecular biology of the cell25(12)1892-904(2014 Jun)

Analysis of ESCRT functions in exosome biogenesis, composition and secretion highlights the heterogeneity of extracellular vesicles.
Colombo M, Moita C, van Niel G, Kowal J, Vigneron J, Benaroch P, Manel N, Moita LF, Th?ry C, Raposo G
Journal of cell science126(Pt 24)5553-65(2013 Dec)

Hrs controls sorting of the epithelial Na+ channel between endosomal degradation and recycling pathways.
Zhou R, Kabra R, Olson DR, Piper RC, Snyder PM
The Journal of biological chemistry285(40)30523-30(2010 Oct)

Ubiquitylation of the gap junction protein connexin-43 signals its trafficking from early endosomes to lysosomes in a process mediated by Hrs and Tsg101.
Leithe E, Kjenseth A, Sirnes S, Stenmark H, Brech A, Rivedal E
Journal of cell science122(Pt 21)3883-93(2009 Nov)

How do ESCRT proteins control autophagy?
Rusten TE, Stenmark H
Journal of cell science122(Pt 13)2179-83(2009 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Nedd4-2 induces binding of ENaC to Hrs, which mediates the sorting decision between ENaC degradation and recycling.
Title: Hrs controls sorting of the epithelial Na+ channel between endosomal degradation and recycling pathways.

Hrs and Tsg101 as crucial regulators of trafficking of Cx43 to lysosomes.
Title: Ubiquitylation of the gap junction protein connexin-43 signals its trafficking from early endosomes to lysosomes in a process mediated by Hrs and Tsg101.

The distinct role of Hrs in promoting recycling of internalized TrkB-FL receptors is independent of its ubiquitin-interacting motif.
Title: Essential role of Hrs in endocytic recycling of full-length TrkB receptor but not its isoform TrkB.T1.

GRIF1 may participate in microtubule-based transport of early endosomes by acting as an adaptor linking Hrs-containing endosomes to kinesin.
Title: GRIF1 binds Hrs and is a new regulator of endosomal trafficking.

HRS can reduce the abundance of total and active EGFR and may reflect cell type-specific HRS function
Title: HRS inhibits EGF receptor signaling in the RT4 rat schwannoma cell line.

The following Hgs gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Hgs cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
ORa04632	NM_019387.2 Latest version!	Rattus norvegicus hepatocyte growth factor-regulated tyrosine kinase substrate (Hgs), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5-7	$391.30 $559.00
ORa34045	XM_006247908.3 Latest version!	Rattus norvegicus hepatocyte growth factor-regulated tyrosine kinase substrate (Hgs), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$475.30 $679.00
ORa34046	XM_006247909.3 Latest version!	Rattus norvegicus hepatocyte growth factor-regulated tyrosine kinase substrate (Hgs), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$475.30 $679.00

ORF Online Only Promotion

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Hgs ( NM_019387.2 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> NP_062260.2 Rattus norvegicus hepatocyte growth factor-regulated tyrosine kinase substrate (Hgs), mRNA.

Price & Availability↑

CloneID	ORa04632
Clone ID Related Accession (Same CDS sequence)	NM_019387.2
Accession Version	NM_019387.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2316bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-09-23
Organism	Rattus norvegicus(Norway rat)
Product	hepatocyte growth factor-regulated tyrosine kinase substrate
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from BC083561.1. On Oct 12, 2005 this sequence version replaced NM_019387.1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC083561.1, AB002811.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMD00052296, SAMD00052297 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGGGCGAG GCAGCGGCAC CTTCGAGCGT CTCCTAGACA AAGCCACCAG CCAGCTTCTA 
TTGGAGACAG ACTGGGAGTC CATTCTACAG ATCTGCGACC TGATCCGTCA GGGGGACACA 
CAAGCAAAAT ATGCTGTAAA CTCCATCAAG AAGAAGGTCA ATGATAAGAA CCCACATGTG 
GCTTTGTATG CTCTGGAGGT GATGGAGTCT GTGGTAAAGA ACTGTGGCCA GACAGTCCAT 
GATGAAGTGG CCAACAAACA GACCATGGAA GAACTGAAGG AGCTGCTGAA GAGGCAAGTG 
GAAGTTAATG TTCGGAACAA GATCTTGTAC CTGATCCAGG CCTGGGCACA TGCGTTCCGG 
AATGAACCCA AGTACAAGGT GGTCCAGGAC ACATACCAGA TCATGAAGGT AGAAGGACAT 
GTCTTCCCTG AGTTTAAGGA GAGCGACGCC ATGTTTGCTG CTGAAAGAGC CCCTGACTGG 
GTGGATGCTG AGGAGTGCCA TCGGTGCAGA GTACAGTTTG GAGTGGTGAC CCGCAAGCAT 
CACTGCCGAG CGTGTGGGCA GATCTTTTGT GGCAAGTGTT CCTCCAAGTA CTCCACCATC 
CCCAAGTTCG GCATTGAGAA GGAAGTGCGC GTGTGTGAGC CCTGCTATGA GCAGCTGAAC 
AAGAAGGCAG AAGGGAAAGC TGCCTCTACC ACTGAGCTGC CCCCAGAGTA CCTGACCAGC 
CCCCTGTCAC AGCAGTCTCA GCTGCCCCCA AAGCGGGATG AGACAGCACT GCAAGAAGAG 
GAGGAGCTAC AGCTGGCGCT GGCCCTATCA CAGTCAGAGG CTGAGGAGAA GGAAAGGATG 
AGACAGAAGT CAACATACAC AGCGCATCCA AAGTCAGAGC CTGCGCCCTT GGCTTCCTCT 
GCACCCCCAG CTGGTAGCCT GTACTCCTCG CCTGTGAACT CATCAGCACC TCTGGCTGAG 
GACATCGACC CTGAGCTTGC AAGATACCTC AACCGGAACT ACTGGGAGAA GAAACAGGAA 
GAAGCTCGGA AGAGCCCCAC ACCATCTGCA CCTGTGCCCC TGACCGAGCC AGCTGCCCAG 
CCCGGAGAAG GACATACAGC CCCCAACAGC ATGGTAGAGG CCCCTCTTCC AGAGACAGAC 
TCTCAGCCCA TAACTTCCTG CAGTGGCCCC TTTAGTGAGC AGTACCAGAA CGGGGAGTCG 
GAGGAGAGCC ACGAGCAGTT CCTCAAGGCC CTGCAGAATG CAGTCAGCAC TTTTGTCAAC 
CGCATGAAGA GCAACCACAT GAGGGGCCGC AGTATCACCA ATGACTCGGC TGTGCTGTCC 
CTCTTCCAGT CCATCAATAG CACACACCCA CAGCTGCTCG AGCTGCTCAA CCGGCTGGAT 
GAGCGCAGGC TGTACTACGA GGGGCTTCAG GACAAGCTGG CACAGATACG TGATGCCCGA 
GGGGCCCTGA GTGCCCTGCG TGAAGAGCAC AGGGAGAAGC TGCGCCGGGC AGCTGAGGAG 
GCGGAGCGTC AACGTCAGAT CCAGCTGGCA CAGAAGCTGG AGATCATGAG ACAAAAGAAG 
CAGGAGTATC TGGAGGTGCA GAGACAGCTA GCTATCCAGC GTCTGCAGGA ACAGGAGAAG 
GAACGGCAGA TGCGCCTGGA GCAACAGAAG CAGACTGTCC AGATGCGTGC CCAGATGCCT 
GCCTTCCCCT TGCCTTATGC CCAGCTCCAG GCTATGCCCA CAGCTGGGGG TGTACTCTAC 
CAGCCCTCAG GCCCAACCAG CTTTCCTGGC ACCTTTAGCC CAGCAGGCTC AGTAGAGGGC 
TCTCCGATGC ATGGTGTGTA TATGAGCCAG CCAGCCCCAG CCACTGGCCC CTACCCCAGC 
ATGCCTGGCA CCACAGCAGA TCCCAGCATG GTCAGCGCCT ACATGTACCC AGCAGGTGCC 
CCTGGGGCAC AGGCAGCCCC TCAGGCCCAG GCCGGGCCCA CCACCAACCC TGCCTACTCC 
TCGTACCAGC CTACTCCAAC CCCAGGCTAC CAGGCCCCAC AGAGCCTCCC AGCCATCTCC 
CAGCCTCCAC AGACCAGCAA CATTGGCTAC ATGGGGAGCC AGCCAATGTC CATGGGCTAC 
CAGCCATACA ACATGCAGAA TCTCATGACC ACCCTTCCAG GCCAGGATGC GTCTCTGCCA 
GCCCAGCAGC CCTACATCAC AGGGCAGCAG CCCATGTACC AGCAGATGGC ACCCAGCACT 
GGCCCTCCCC AGCAGCAGCC CCCTGTGGCC CAACCGCCAC CTACACAGGG ACCGCCAGCA 
CAGGGCAATG AGACCCAGCT CATCTCCTTC GACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_062260.2
CDS	15..2330
Translation	MGRGSGTFERLLDKATSQLLLETDWESILQICDLIRQGDTQAKYAVNSIKKKVNDKNPHVALYALEVMESVVKNCGQTVHDEVANKQTMEELKELLKRQVEVNVRNKILYLIQAWAHAFRNEPKYKVVQDTYQIMKVEGHVFPEFKESDAMFAAERAPDWVDAEECHRCRVQFGVVTRKHHCRACGQIFCGKCSSKYSTIPKFGIEKEVRVCEPCYEQLNKKAEGKAASTTELPPEYLTSPLSQQSQLPPKRDETALQEEEELQLALALSQSEAEEKERMRQKSTYTAHPKSEPAPLASSAPPAGSLYSSPVNSSAPLAEDIDPELARYLNRNYWEKKQEEARKSPTPSAPVPLTEPAAQPGEGHTAPNSMVEAPLPETDSQPITSCSGPFSEQYQNGESEESHEQFLKALQNAVSTFVNRMKSNHMRGRSITNDSAVLSLFQSINSTHPQLLELLNRLDERRLYYEGLQDKLAQIRDARGALSALREEHREKLRRAAEEAERQRQIQLAQKLEIMRQKKQEYLEVQRQLAIQRLQEQEKERQMRLEQQKQTVQMRAQMPAFPLPYAQLQAMPTAGGVLYQPSGPTSFPGTFSPAGSVEGSPMHGVYMSQPAPATGPYPSMPGTTADPSMVSAYMYPAGAPGAQAAPQAQAGPTTNPAYSSYQPTPTPGYQAPQSLPAISQPPQTSNIGYMGSQPMSMGYQPYNMQNLMTTLPGQDASLPAQQPYITGQQPMYQQMAPSTGPPQQQPPVAQPPPTQGPPAQGNETQLISFD

Target ORF information:

RefSeq Version	NM_019387.2
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus hepatocyte growth factor-regulated tyrosine kinase substrate (Hgs), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_019387.2

ORF Insert Sequence:

ATGGGGCGAG GCAGCGGCAC CTTCGAGCGT CTCCTAGACA AAGCCACCAG CCAGCTTCTA 
TTGGAGACAG ACTGGGAGTC CATTCTACAG ATCTGCGACC TGATCCGTCA GGGGGACACA 
CAAGCAAAAT ATGCTGTAAA CTCCATCAAG AAGAAGGTCA ATGATAAGAA CCCACATGTG 
GCTTTGTATG CTCTGGAGGT GATGGAGTCT GTGGTAAAGA ACTGTGGCCA GACAGTCCAT 
GATGAAGTGG CCAACAAACA GACCATGGAA GAACTGAAGG AGCTGCTGAA GAGGCAAGTG 
GAAGTTAATG TTCGGAACAA GATCTTGTAC CTGATCCAGG CCTGGGCACA TGCGTTCCGG 
AATGAACCCA AGTACAAGGT GGTCCAGGAC ACATACCAGA TCATGAAGGT AGAAGGACAT 
GTCTTCCCTG AGTTTAAGGA GAGCGACGCC ATGTTTGCTG CTGAAAGAGC CCCTGACTGG 
GTGGATGCTG AGGAGTGCCA TCGGTGCAGA GTACAGTTTG GAGTGGTGAC CCGCAAGCAT 
CACTGCCGAG CGTGTGGGCA GATCTTTTGT GGCAAGTGTT CCTCCAAGTA CTCCACCATC 
CCCAAGTTCG GCATTGAGAA GGAAGTGCGC GTGTGTGAGC CCTGCTATGA GCAGCTGAAC 
AAGAAGGCAG AAGGGAAAGC TGCCTCTACC ACTGAGCTGC CCCCAGAGTA CCTGACCAGC 
CCCCTGTCAC AGCAGTCTCA GCTGCCCCCA AAGCGGGATG AGACAGCACT GCAAGAAGAG 
GAGGAGCTAC AGCTGGCGCT GGCCCTATCA CAGTCAGAGG CTGAGGAGAA GGAAAGGATG 
AGACAGAAGT CAACATACAC AGCGCATCCA AAGTCAGAGC CTGCGCCCTT GGCTTCCTCT 
GCACCCCCAG CTGGTAGCCT GTACTCCTCG CCTGTGAACT CATCAGCACC TCTGGCTGAG 
GACATCGACC CTGAGCTTGC AAGATACCTC AACCGGAACT ACTGGGAGAA GAAACAGGAA 
GAAGCTCGGA AGAGCCCCAC ACCATCTGCA CCTGTGCCCC TGACCGAGCC AGCTGCCCAG 
CCCGGAGAAG GACATACAGC CCCCAACAGC ATGGTAGAGG CCCCTCTTCC AGAGACAGAC 
TCTCAGCCCA TAACTTCCTG CAGTGGCCCC TTTAGTGAGC AGTACCAGAA CGGGGAGTCG 
GAGGAGAGCC ACGAGCAGTT CCTCAAGGCC CTGCAGAATG CAGTCAGCAC TTTTGTCAAC 
CGCATGAAGA GCAACCACAT GAGGGGCCGC AGTATCACCA ATGACTCGGC TGTGCTGTCC 
CTCTTCCAGT CCATCAATAG CACACACCCA CAGCTGCTCG AGCTGCTCAA CCGGCTGGAT 
GAGCGCAGGC TGTACTACGA GGGGCTTCAG GACAAGCTGG CACAGATACG TGATGCCCGA 
GGGGCCCTGA GTGCCCTGCG TGAAGAGCAC AGGGAGAAGC TGCGCCGGGC AGCTGAGGAG 
GCGGAGCGTC AACGTCAGAT CCAGCTGGCA CAGAAGCTGG AGATCATGAG ACAAAAGAAG 
CAGGAGTATC TGGAGGTGCA GAGACAGCTA GCTATCCAGC GTCTGCAGGA ACAGGAGAAG 
GAACGGCAGA TGCGCCTGGA GCAACAGAAG CAGACTGTCC AGATGCGTGC CCAGATGCCT 
GCCTTCCCCT TGCCTTATGC CCAGCTCCAG GCTATGCCCA CAGCTGGGGG TGTACTCTAC 
CAGCCCTCAG GCCCAACCAG CTTTCCTGGC ACCTTTAGCC CAGCAGGCTC AGTAGAGGGC 
TCTCCGATGC ATGGTGTGTA TATGAGCCAG CCAGCCCCAG CCACTGGCCC CTACCCCAGC 
ATGCCTGGCA CCACAGCAGA TCCCAGCATG GTCAGCGCCT ACATGTACCC AGCAGGTGCC 
CCTGGGGCAC AGGCAGCCCC TCAGGCCCAG GCCGGGCCCA CCACCAACCC TGCCTACTCC 
TCGTACCAGC CTACTCCAAC CCCAGGCTAC CAGGCCCCAC AGAGCCTCCC AGCCATCTCC 
CAGCCTCCAC AGACCAGCAA CATTGGCTAC ATGGGGAGCC AGCCAATGTC CATGGGCTAC 
CAGCCATACA ACATGCAGAA TCTCATGACC ACCCTTCCAG GCCAGGATGC GTCTCTGCCA 
GCCCAGCAGC CCTACATCAC AGGGCAGCAG CCCATGTACC AGCAGATGGC ACCCAGCACT 
GGCCCTCCCC AGCAGCAGCC CCCTGTGGCC CAACCGCCAC CTACACAGGG ACCGCCAGCA 
CAGGGCAATG AGACCCAGCT CATCTCCTTC GACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Hgs ( XM_006247908.3 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_006247970.1 Rattus norvegicus hepatocyte growth factor-regulated tyrosine kinase substrate (Hgs), transcript variant X1, mRNA.

Price & Availability↑

CloneID	ORa34045
Clone ID Related Accession (Same CDS sequence)	XM_006247908.3
Accession Version	XM_006247908.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2517bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	hepatocyte growth factor-regulated tyrosine kinase substrate isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005109.4) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jul 26, 2016 this sequence version replaced XM_006247908.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGCGGTC CAATGGACGC TCAGAGTCCG TGTGCCAGCG CACGGCTCGT CTCGGAGCAG 
GACTCGGGGC GTCGCGGAGA TATTGCGCGT GCGCGAGCAG AAAAAGAAAG GCGGAAGCGG 
AAGTTGGGGG GCGCGTCAGC TCCGAAAGAG GGAGCGCCCG CGGCGTCGGT CCGGAGTGGG 
GTCGCCATGG GGCGAGGCAG CGGCACCTTC GAGCGTCTCC TAGACAAAGC CACCAGCCAG 
CTTCTATTGG AGACAGACTG GGAGTCCATT CTACAGATCT GCGACCTGAT CCGTCAGGGG 
GACACACAAG CAAAATATGC TGTAAACTCC ATCAAGAAGA AGGTCAATGA TAAGAACCCA 
CATGTGGCTT TGTATGCTCT GGAGGTGATG GAGTCTGTGG TAAAGAACTG TGGCCAGACA 
GTCCATGATG AAGTGGCCAA CAAACAGACC ATGGAAGAAC TGAAGGAGCT GCTGAAGAGG 
CAAGTGGAAG TTAATGTTCG GAACAAGATC TTGTACCTGA TCCAGGCCTG GGCACATGCG 
TTCCGGAATG AACCCAAGTA CAAGGTGGTC CAGGACACAT ACCAGATCAT GAAGGTAGAA 
GGACATGTCT TCCCTGAGTT TAAGGAGAGC GACGCCATGT TTGCTGCTGA AAGAGCCCCT 
GACTGGGTGG ATGCTGAGGA GTGCCATCGG TGCAGAGTAC AGTTTGGAGT GGTGACCCGC 
AAGCATCACT GCCGAGCGTG TGGGCAGATC TTTTGTGGCA AGTGTTCCTC CAAGTACTCC 
ACCATCCCCA AGTTCGGCAT TGAGAAGGAA GTGCGCGTGT GTGAGCCCTG CTATGAGCAG 
CTGAACAAGA AGGCAGAAGG GAAAGCTGCC TCTACCACTG AGCTGCCCCC AGAGTACCTG 
ACCAGCCCCC TGTCACAGCA GTCTCAGCTG CCCCCAAAGC GGGATGAGAC AGCACTGCAA 
GAAGAGGAGG AGCTACAGCT GGCGCTGGCC CTATCACAGT CAGAGGCTGA GGAGAAGGAA 
AGGATGAGAC AGAAGTCAAC ATACACAGCG CATCCAAAGT CAGAGCCTGC GCCCTTGGCT 
TCCTCTGCAC CCCCAGCTGG TAGCCTGTAC TCCTCGCCTG TGAACTCATC AGCACCTCTG 
GCTGAGGACA TCGACCCTGA GCTTGCAAGA TACCTCAACC GGAACTACTG GGAGAAGAAA 
CAGGAAGAAG CTCGGAAGAG CCCCACACCA TCTGCACCTG TGCCCCTGAC CGAGCCAGCT 
GCCCAGCCCG GAGAAGGACA TACAGCCCCC AACAGCATGG TAGAGGCCCC TCTTCCAGAG 
ACAGACTCTC AGCCCATAAC TTCCTGCAGT GGCCCCTTTA GTGAGCAGTA CCAGAACGGG 
GAGTCGGAGG AGAGCCACGA GCAGTTCCTC AAGGCCCTGC AGAATGCAGT CAGCACTTTT 
GTCAACCGCA TGAAGAGCAA CCACATGAGG GGCCGCAGTA TCACCAATGA CTCGGCTGTG 
CTGTCCCTCT TCCAGTCCAT CAATAGCACA CACCCACAGC TGCTCGAGCT GCTCAACCGG 
CTGGATGAGC GCAGGCTGTA CTACGAGGGG CTTCAGGACA AGCTGGCACA GATACGTGAT 
GCCCGAGGGG CCCTGAGTGC CCTGCGTGAA GAGCACAGGG AGAAGCTGCG CCGGGCAGCT 
GAGGAGGCGG AGCGTCAACG TCAGATCCAG CTGGCACAGA AGCTGGAGAT CATGAGACAA 
AAGAAGCAGG AGTATCTGGA GGTGCAGAGA CAGCTAGCTA TCCAGCGTCT GCAGGAACAG 
GAGAAGGAAC GGCAGATGCG CCTGGAGCAA CAGAAGCAGA CTGTCCAGAT GCGTGCCCAG 
ATGCCTGCCT TCCCCTTGCC TTATGCCCAG CTCCAGGCTA TGCCCACAGC TGGGGGTGTA 
CTCTACCAGC CCTCAGGCCC AACCAGCTTT CCTGGCACCT TTAGCCCAGC AGGCTCAGTA 
GAGGGCTCTC CGATGCATGG TGTGTATATG AGCCAGCCAG CCCCAGCCAC TGGCCCCTAC 
CCCAGCATGC CTGGCACCAC AGCAGATCCC AGCATGGTCA GCGCCTACAT GTACCCAGCA 
GGTGCCCCTG GGGCACAGGC AGCCCCTCAG GCCCAGGCCG GGCCCACCAC CAACCCTGCC 
TACTCCTCGT ACCAGCCTAC TCCAACCCCA GGCTACCAGA ATGTGGCTTC TCAGGCCCCA 
CAGAGCCTCC CAGCCATCTC CCAGCCTCCA CAGACCAGCA ACATTGGCTA CATGGGGAGC 
CAGCCAATGT CCATGGGCTA CCAGCCATAC AACATGCAGA ATCTCATGAC CACCCTTCCA 
GGCCAGGATG CGTCTCTGCC AGCCCAGCAG CCCTACATCA CAGGGCAGCA GCCCATGTAC 
CAGCAGATGG CACCCAGCAC TGGCCCTCCC CAGCAGCAGC CCCCTGTGGC CCAACCGCCA 
CCTACACAGG GACCGCCAGC ACAGGGCAAT GAGACCCAGC TCATCTCCTT CGACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006247970.1
CDS	23..2539
Translation	MSGPMDAQSPCASARLVSEQDSGRRGDIARARAEKERRKRKLGGASAPKEGAPAASVRSGVAMGRGSGTFERLLDKATSQLLLETDWESILQICDLIRQGDTQAKYAVNSIKKKVNDKNPHVALYALEVMESVVKNCGQTVHDEVANKQTMEELKELLKRQVEVNVRNKILYLIQAWAHAFRNEPKYKVVQDTYQIMKVEGHVFPEFKESDAMFAAERAPDWVDAEECHRCRVQFGVVTRKHHCRACGQIFCGKCSSKYSTIPKFGIEKEVRVCEPCYEQLNKKAEGKAASTTELPPEYLTSPLSQQSQLPPKRDETALQEEEELQLALALSQSEAEEKERMRQKSTYTAHPKSEPAPLASSAPPAGSLYSSPVNSSAPLAEDIDPELARYLNRNYWEKKQEEARKSPTPSAPVPLTEPAAQPGEGHTAPNSMVEAPLPETDSQPITSCSGPFSEQYQNGESEESHEQFLKALQNAVSTFVNRMKSNHMRGRSITNDSAVLSLFQSINSTHPQLLELLNRLDERRLYYEGLQDKLAQIRDARGALSALREEHREKLRRAAEEAERQRQIQLAQKLEIMRQKKQEYLEVQRQLAIQRLQEQEKERQMRLEQQKQTVQMRAQMPAFPLPYAQLQAMPTAGGVLYQPSGPTSFPGTFSPAGSVEGSPMHGVYMSQPAPATGPYPSMPGTTADPSMVSAYMYPAGAPGAQAAPQAQAGPTTNPAYSSYQPTPTPGYQNVASQAPQSLPAISQPPQTSNIGYMGSQPMSMGYQPYNMQNLMTTLPGQDASLPAQQPYITGQQPMYQQMAPSTGPPQQQPPVAQPPPTQGPPAQGNETQLISFD

Target ORF information:

RefSeq Version	XM_006247908.3
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus hepatocyte growth factor-regulated tyrosine kinase substrate (Hgs), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006247908.3

ORF Insert Sequence:

ATGAGCGGTC CAATGGACGC TCAGAGTCCG TGTGCCAGCG CACGGCTCGT CTCGGAGCAG 
GACTCGGGGC GTCGCGGAGA TATTGCGCGT GCGCGAGCAG AAAAAGAAAG GCGGAAGCGG 
AAGTTGGGGG GCGCGTCAGC TCCGAAAGAG GGAGCGCCCG CGGCGTCGGT CCGGAGTGGG 
GTCGCCATGG GGCGAGGCAG CGGCACCTTC GAGCGTCTCC TAGACAAAGC CACCAGCCAG 
CTTCTATTGG AGACAGACTG GGAGTCCATT CTACAGATCT GCGACCTGAT CCGTCAGGGG 
GACACACAAG CAAAATATGC TGTAAACTCC ATCAAGAAGA AGGTCAATGA TAAGAACCCA 
CATGTGGCTT TGTATGCTCT GGAGGTGATG GAGTCTGTGG TAAAGAACTG TGGCCAGACA 
GTCCATGATG AAGTGGCCAA CAAACAGACC ATGGAAGAAC TGAAGGAGCT GCTGAAGAGG 
CAAGTGGAAG TTAATGTTCG GAACAAGATC TTGTACCTGA TCCAGGCCTG GGCACATGCG 
TTCCGGAATG AACCCAAGTA CAAGGTGGTC CAGGACACAT ACCAGATCAT GAAGGTAGAA 
GGACATGTCT TCCCTGAGTT TAAGGAGAGC GACGCCATGT TTGCTGCTGA AAGAGCCCCT 
GACTGGGTGG ATGCTGAGGA GTGCCATCGG TGCAGAGTAC AGTTTGGAGT GGTGACCCGC 
AAGCATCACT GCCGAGCGTG TGGGCAGATC TTTTGTGGCA AGTGTTCCTC CAAGTACTCC 
ACCATCCCCA AGTTCGGCAT TGAGAAGGAA GTGCGCGTGT GTGAGCCCTG CTATGAGCAG 
CTGAACAAGA AGGCAGAAGG GAAAGCTGCC TCTACCACTG AGCTGCCCCC AGAGTACCTG 
ACCAGCCCCC TGTCACAGCA GTCTCAGCTG CCCCCAAAGC GGGATGAGAC AGCACTGCAA 
GAAGAGGAGG AGCTACAGCT GGCGCTGGCC CTATCACAGT CAGAGGCTGA GGAGAAGGAA 
AGGATGAGAC AGAAGTCAAC ATACACAGCG CATCCAAAGT CAGAGCCTGC GCCCTTGGCT 
TCCTCTGCAC CCCCAGCTGG TAGCCTGTAC TCCTCGCCTG TGAACTCATC AGCACCTCTG 
GCTGAGGACA TCGACCCTGA GCTTGCAAGA TACCTCAACC GGAACTACTG GGAGAAGAAA 
CAGGAAGAAG CTCGGAAGAG CCCCACACCA TCTGCACCTG TGCCCCTGAC CGAGCCAGCT 
GCCCAGCCCG GAGAAGGACA TACAGCCCCC AACAGCATGG TAGAGGCCCC TCTTCCAGAG 
ACAGACTCTC AGCCCATAAC TTCCTGCAGT GGCCCCTTTA GTGAGCAGTA CCAGAACGGG 
GAGTCGGAGG AGAGCCACGA GCAGTTCCTC AAGGCCCTGC AGAATGCAGT CAGCACTTTT 
GTCAACCGCA TGAAGAGCAA CCACATGAGG GGCCGCAGTA TCACCAATGA CTCGGCTGTG 
CTGTCCCTCT TCCAGTCCAT CAATAGCACA CACCCACAGC TGCTCGAGCT GCTCAACCGG 
CTGGATGAGC GCAGGCTGTA CTACGAGGGG CTTCAGGACA AGCTGGCACA GATACGTGAT 
GCCCGAGGGG CCCTGAGTGC CCTGCGTGAA GAGCACAGGG AGAAGCTGCG CCGGGCAGCT 
GAGGAGGCGG AGCGTCAACG TCAGATCCAG CTGGCACAGA AGCTGGAGAT CATGAGACAA 
AAGAAGCAGG AGTATCTGGA GGTGCAGAGA CAGCTAGCTA TCCAGCGTCT GCAGGAACAG 
GAGAAGGAAC GGCAGATGCG CCTGGAGCAA CAGAAGCAGA CTGTCCAGAT GCGTGCCCAG 
ATGCCTGCCT TCCCCTTGCC TTATGCCCAG CTCCAGGCTA TGCCCACAGC TGGGGGTGTA 
CTCTACCAGC CCTCAGGCCC AACCAGCTTT CCTGGCACCT TTAGCCCAGC AGGCTCAGTA 
GAGGGCTCTC CGATGCATGG TGTGTATATG AGCCAGCCAG CCCCAGCCAC TGGCCCCTAC 
CCCAGCATGC CTGGCACCAC AGCAGATCCC AGCATGGTCA GCGCCTACAT GTACCCAGCA 
GGTGCCCCTG GGGCACAGGC AGCCCCTCAG GCCCAGGCCG GGCCCACCAC CAACCCTGCC 
TACTCCTCGT ACCAGCCTAC TCCAACCCCA GGCTACCAGA ATGTGGCTTC TCAGGCCCCA 
CAGAGCCTCC CAGCCATCTC CCAGCCTCCA CAGACCAGCA ACATTGGCTA CATGGGGAGC 
CAGCCAATGT CCATGGGCTA CCAGCCATAC AACATGCAGA ATCTCATGAC CACCCTTCCA 
GGCCAGGATG CGTCTCTGCC AGCCCAGCAG CCCTACATCA CAGGGCAGCA GCCCATGTAC 
CAGCAGATGG CACCCAGCAC TGGCCCTCCC CAGCAGCAGC CCCCTGTGGC CCAACCGCCA 
CCTACACAGG GACCGCCAGC ACAGGGCAAT GAGACCCAGC TCATCTCCTT CGACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Hgs ( XM_006247909.3 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_006247971.1 Rattus norvegicus hepatocyte growth factor-regulated tyrosine kinase substrate (Hgs), transcript variant X2, mRNA.

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CloneID	ORa34046
Clone ID Related Accession (Same CDS sequence)	XM_006247909.3
Accession Version	XM_006247909.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2514bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	hepatocyte growth factor-regulated tyrosine kinase substrate isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005109.4) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jul 26, 2016 this sequence version replaced XM_006247909.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGCGGTC CAATGGACGC TCAGAGTCCG TGTGCCAGCG CACGGCTCGT CTCGGAGCAG 
GACTCGGGGC GTCGCGGAGA TATTGCGCGT GCGCGAGCAG AAAAAGAAAG GCGGAAGCGG 
AAGTTGGGGG GCGCGTCAGC TCCGAAAGAG GGAGCGCCCG CGGCGTCGGT CCGGAGTGGG 
GTCGCCATGG GGCGAGGCAG CGGCACCTTC GAGCGTCTCC TAGACAAAGC CACCAGCCAG 
CTTCTATTGG AGACAGACTG GGAGTCCATT CTACAGATCT GCGACCTGAT CCGTCAGGGG 
GACACACAAG CAAAATATGC TGTAAACTCC ATCAAGAAGA AGGTCAATGA TAAGAACCCA 
CATGTGGCTT TGTATGCTCT GGAGGTGATG GAGTCTGTGG TAAAGAACTG TGGCCAGACA 
GTCCATGATG AAGTGGCCAA CAAACAGACC ATGGAAGAAC TGAAGGAGCT GCTGAAGAGG 
CAAGTGGAAG TTAATGTTCG GAACAAGATC TTGTACCTGA TCCAGGCCTG GGCACATGCG 
TTCCGGAATG AACCCAAGTA CAAGGTGGTC CAGGACACAT ACCAGATCAT GAAGGTAGAA 
GGACATGTCT TCCCTGAGTT TAAGGAGAGC GACGCCATGT TTGCTGCTGA AAGAGCCCCT 
GACTGGGTGG ATGCTGAGGA GTGCCATCGG TGCAGAGTAC AGTTTGGAGT GGTGACCCGC 
AAGCATCACT GCCGAGCGTG TGGGCAGATC TTTTGTGGCA AGTGTTCCTC CAAGTACTCC 
ACCATCCCCA AGTTCGGCAT TGAGAAGGAA GTGCGCGTGT GTGAGCCCTG CTATGAGCAG 
CTGAACAAGA AGGCAGAAGG GAAAGCTGCC TCTACCACTG AGCTGCCCCC AGAGTACCTG 
ACCAGCCCCC TGTCACAGCA GTCTCAGCTG CCCCCAAAGC GGGATGAGAC AGCACTGCAA 
GAAGAGGAGG AGCTACAGCT GGCGCTGGCC CTATCACAGT CAGAGGCTGA GGAGAAGGAA 
AGGATGAGAC AGAAGTCAAC ATACACAGCG CATCCAAAGT CAGAGCCTGC GCCCTTGGCT 
TCCTCTGCAC CCCCAGCTGG TAGCCTGTAC TCCTCGCCTG TGAACTCATC AGCACCTCTG 
GCTGAGGACA TCGACCCTGA GCTTGCAAGA TACCTCAACC GGAACTACTG GGAGAAGAAA 
CAGGAAGAAG CTCGGAAGAG CCCCACACCA TCTGCACCTG TGCCCCTGAC CGAGCCAGCT 
GCCCAGCCCG GAGAAGGACA TACAGCCCCC AACAGCATGG TAGAGGCCCC TCTTCCAGAG 
ACAGACTCTC AGCCCATAAC TTCCTGCAGT GGCCCCTTTA GTGAGTACCA GAACGGGGAG 
TCGGAGGAGA GCCACGAGCA GTTCCTCAAG GCCCTGCAGA ATGCAGTCAG CACTTTTGTC 
AACCGCATGA AGAGCAACCA CATGAGGGGC CGCAGTATCA CCAATGACTC GGCTGTGCTG 
TCCCTCTTCC AGTCCATCAA TAGCACACAC CCACAGCTGC TCGAGCTGCT CAACCGGCTG 
GATGAGCGCA GGCTGTACTA CGAGGGGCTT CAGGACAAGC TGGCACAGAT ACGTGATGCC 
CGAGGGGCCC TGAGTGCCCT GCGTGAAGAG CACAGGGAGA AGCTGCGCCG GGCAGCTGAG 
GAGGCGGAGC GTCAACGTCA GATCCAGCTG GCACAGAAGC TGGAGATCAT GAGACAAAAG 
AAGCAGGAGT ATCTGGAGGT GCAGAGACAG CTAGCTATCC AGCGTCTGCA GGAACAGGAG 
AAGGAACGGC AGATGCGCCT GGAGCAACAG AAGCAGACTG TCCAGATGCG TGCCCAGATG 
CCTGCCTTCC CCTTGCCTTA TGCCCAGCTC CAGGCTATGC CCACAGCTGG GGGTGTACTC 
TACCAGCCCT CAGGCCCAAC CAGCTTTCCT GGCACCTTTA GCCCAGCAGG CTCAGTAGAG 
GGCTCTCCGA TGCATGGTGT GTATATGAGC CAGCCAGCCC CAGCCACTGG CCCCTACCCC 
AGCATGCCTG GCACCACAGC AGATCCCAGC ATGGTCAGCG CCTACATGTA CCCAGCAGGT 
GCCCCTGGGG CACAGGCAGC CCCTCAGGCC CAGGCCGGGC CCACCACCAA CCCTGCCTAC 
TCCTCGTACC AGCCTACTCC AACCCCAGGC TACCAGAATG TGGCTTCTCA GGCCCCACAG 
AGCCTCCCAG CCATCTCCCA GCCTCCACAG ACCAGCAACA TTGGCTACAT GGGGAGCCAG 
CCAATGTCCA TGGGCTACCA GCCATACAAC ATGCAGAATC TCATGACCAC CCTTCCAGGC 
CAGGATGCGT CTCTGCCAGC CCAGCAGCCC TACATCACAG GGCAGCAGCC CATGTACCAG 
CAGATGGCAC CCAGCACTGG CCCTCCCCAG CAGCAGCCCC CTGTGGCCCA ACCGCCACCT 
ACACAGGGAC CGCCAGCACA GGGCAATGAG ACCCAGCTCA TCTCCTTCGA CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006247971.1
CDS	23..2536
Translation	MSGPMDAQSPCASARLVSEQDSGRRGDIARARAEKERRKRKLGGASAPKEGAPAASVRSGVAMGRGSGTFERLLDKATSQLLLETDWESILQICDLIRQGDTQAKYAVNSIKKKVNDKNPHVALYALEVMESVVKNCGQTVHDEVANKQTMEELKELLKRQVEVNVRNKILYLIQAWAHAFRNEPKYKVVQDTYQIMKVEGHVFPEFKESDAMFAAERAPDWVDAEECHRCRVQFGVVTRKHHCRACGQIFCGKCSSKYSTIPKFGIEKEVRVCEPCYEQLNKKAEGKAASTTELPPEYLTSPLSQQSQLPPKRDETALQEEEELQLALALSQSEAEEKERMRQKSTYTAHPKSEPAPLASSAPPAGSLYSSPVNSSAPLAEDIDPELARYLNRNYWEKKQEEARKSPTPSAPVPLTEPAAQPGEGHTAPNSMVEAPLPETDSQPITSCSGPFSEYQNGESEESHEQFLKALQNAVSTFVNRMKSNHMRGRSITNDSAVLSLFQSINSTHPQLLELLNRLDERRLYYEGLQDKLAQIRDARGALSALREEHREKLRRAAEEAERQRQIQLAQKLEIMRQKKQEYLEVQRQLAIQRLQEQEKERQMRLEQQKQTVQMRAQMPAFPLPYAQLQAMPTAGGVLYQPSGPTSFPGTFSPAGSVEGSPMHGVYMSQPAPATGPYPSMPGTTADPSMVSAYMYPAGAPGAQAAPQAQAGPTTNPAYSSYQPTPTPGYQNVASQAPQSLPAISQPPQTSNIGYMGSQPMSMGYQPYNMQNLMTTLPGQDASLPAQQPYITGQQPMYQQMAPSTGPPQQQPPVAQPPPTQGPPAQGNETQLISFD

Target ORF information:

RefSeq Version	XM_006247909.3
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus hepatocyte growth factor-regulated tyrosine kinase substrate (Hgs), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006247909.3

ORF Insert Sequence:

ATGAGCGGTC CAATGGACGC TCAGAGTCCG TGTGCCAGCG CACGGCTCGT CTCGGAGCAG 
GACTCGGGGC GTCGCGGAGA TATTGCGCGT GCGCGAGCAG AAAAAGAAAG GCGGAAGCGG 
AAGTTGGGGG GCGCGTCAGC TCCGAAAGAG GGAGCGCCCG CGGCGTCGGT CCGGAGTGGG 
GTCGCCATGG GGCGAGGCAG CGGCACCTTC GAGCGTCTCC TAGACAAAGC CACCAGCCAG 
CTTCTATTGG AGACAGACTG GGAGTCCATT CTACAGATCT GCGACCTGAT CCGTCAGGGG 
GACACACAAG CAAAATATGC TGTAAACTCC ATCAAGAAGA AGGTCAATGA TAAGAACCCA 
CATGTGGCTT TGTATGCTCT GGAGGTGATG GAGTCTGTGG TAAAGAACTG TGGCCAGACA 
GTCCATGATG AAGTGGCCAA CAAACAGACC ATGGAAGAAC TGAAGGAGCT GCTGAAGAGG 
CAAGTGGAAG TTAATGTTCG GAACAAGATC TTGTACCTGA TCCAGGCCTG GGCACATGCG 
TTCCGGAATG AACCCAAGTA CAAGGTGGTC CAGGACACAT ACCAGATCAT GAAGGTAGAA 
GGACATGTCT TCCCTGAGTT TAAGGAGAGC GACGCCATGT TTGCTGCTGA AAGAGCCCCT 
GACTGGGTGG ATGCTGAGGA GTGCCATCGG TGCAGAGTAC AGTTTGGAGT GGTGACCCGC 
AAGCATCACT GCCGAGCGTG TGGGCAGATC TTTTGTGGCA AGTGTTCCTC CAAGTACTCC 
ACCATCCCCA AGTTCGGCAT TGAGAAGGAA GTGCGCGTGT GTGAGCCCTG CTATGAGCAG 
CTGAACAAGA AGGCAGAAGG GAAAGCTGCC TCTACCACTG AGCTGCCCCC AGAGTACCTG 
ACCAGCCCCC TGTCACAGCA GTCTCAGCTG CCCCCAAAGC GGGATGAGAC AGCACTGCAA 
GAAGAGGAGG AGCTACAGCT GGCGCTGGCC CTATCACAGT CAGAGGCTGA GGAGAAGGAA 
AGGATGAGAC AGAAGTCAAC ATACACAGCG CATCCAAAGT CAGAGCCTGC GCCCTTGGCT 
TCCTCTGCAC CCCCAGCTGG TAGCCTGTAC TCCTCGCCTG TGAACTCATC AGCACCTCTG 
GCTGAGGACA TCGACCCTGA GCTTGCAAGA TACCTCAACC GGAACTACTG GGAGAAGAAA 
CAGGAAGAAG CTCGGAAGAG CCCCACACCA TCTGCACCTG TGCCCCTGAC CGAGCCAGCT 
GCCCAGCCCG GAGAAGGACA TACAGCCCCC AACAGCATGG TAGAGGCCCC TCTTCCAGAG 
ACAGACTCTC AGCCCATAAC TTCCTGCAGT GGCCCCTTTA GTGAGTACCA GAACGGGGAG 
TCGGAGGAGA GCCACGAGCA GTTCCTCAAG GCCCTGCAGA ATGCAGTCAG CACTTTTGTC 
AACCGCATGA AGAGCAACCA CATGAGGGGC CGCAGTATCA CCAATGACTC GGCTGTGCTG 
TCCCTCTTCC AGTCCATCAA TAGCACACAC CCACAGCTGC TCGAGCTGCT CAACCGGCTG 
GATGAGCGCA GGCTGTACTA CGAGGGGCTT CAGGACAAGC TGGCACAGAT ACGTGATGCC 
CGAGGGGCCC TGAGTGCCCT GCGTGAAGAG CACAGGGAGA AGCTGCGCCG GGCAGCTGAG 
GAGGCGGAGC GTCAACGTCA GATCCAGCTG GCACAGAAGC TGGAGATCAT GAGACAAAAG 
AAGCAGGAGT ATCTGGAGGT GCAGAGACAG CTAGCTATCC AGCGTCTGCA GGAACAGGAG 
AAGGAACGGC AGATGCGCCT GGAGCAACAG AAGCAGACTG TCCAGATGCG TGCCCAGATG 
CCTGCCTTCC CCTTGCCTTA TGCCCAGCTC CAGGCTATGC CCACAGCTGG GGGTGTACTC 
TACCAGCCCT CAGGCCCAAC CAGCTTTCCT GGCACCTTTA GCCCAGCAGG CTCAGTAGAG 
GGCTCTCCGA TGCATGGTGT GTATATGAGC CAGCCAGCCC CAGCCACTGG CCCCTACCCC 
AGCATGCCTG GCACCACAGC AGATCCCAGC ATGGTCAGCG CCTACATGTA CCCAGCAGGT 
GCCCCTGGGG CACAGGCAGC CCCTCAGGCC CAGGCCGGGC CCACCACCAA CCCTGCCTAC 
TCCTCGTACC AGCCTACTCC AACCCCAGGC TACCAGAATG TGGCTTCTCA GGCCCCACAG 
AGCCTCCCAG CCATCTCCCA GCCTCCACAG ACCAGCAACA TTGGCTACAT GGGGAGCCAG 
CCAATGTCCA TGGGCTACCA GCCATACAAC ATGCAGAATC TCATGACCAC CCTTCCAGGC 
CAGGATGCGT CTCTGCCAGC CCAGCAGCCC TACATCACAG GGCAGCAGCC CATGTACCAG 
CAGATGGCAC CCAGCACTGG CCCTCCCCAG CAGCAGCCCC CTGTGGCCCA ACCGCCACCT 
ACACAGGGAC CGCCAGCACA GGGCAATGAG ACCCAGCTCA TCTCCTTCGA CTGA