Aprt cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene
Clones
gRNAs

Summary

Gene Symbol	Aprt
Entrez Gene ID	292072
Full Name	adenine phosphoribosyl transferase
General protein information	Preferred Names adenine phosphoribosyl transferase Names adenine phosphoribosyltransferase
Gene Type	protein-coding
Organism	Rattus norvegicus(Norway rat)
Genome	Chromosome: 19 Map Location: 19q12

mRNA and Protein(s)

mRNA	Protein	Name
NM_001013061.1	NP_001013079.1	adenine phosphoribosyltransferase

Pathways from BioSystems

Homologies

Eremothecium gossypii	AGOS_AER325W	NP_985181.1
Magnaporthe oryzae (rice blast fungus)	MGG_17399	XP_003718359.1
Canis lupus familiaris (dog)	APRT	XP_536752.2
Mus musculus (house mouse)	Aprt	NP_033828.2
Danio rerio (zebrafish)	aprt	NP_956962.1
Saccharomyces cerevisiae (baker's yeast)	APT1	NP_013690.1
Neurospora crassa	NCU02090	XP_963727.2
Xenopus tropicalis (tropical clawed frog)	aprt	NP_001007941.1
Homo sapiens (human)	APRT	NP_000476.1
	APRT	XP_001089867.1
Bos taurus (cattle)	APRT	NP_001020505.1
Gallus gallus (chicken)	APRT	NP_001264576.1
Drosophila melanogaster (fruit fly)	Aprt	NP_476637.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP005723	XP_315739.4
Kluyveromyces lactis	KLLA0B01309g	XP_451589.1
Schizosaccharomyces pombe (fission yeast)	apt1	NP_594433.1
Oryza sativa (rice)	Os12g0589100	NP_001067164.1
Pan troglodytes (chimpanzee)	APRT	XP_003952966.1
Rattus norvegicus (Norway rat)	Aprt	NP_001013079.1
Caenorhabditis elegans (roundworm)	T19B4.3	NP_491663.1
Arabidopsis thaliana (thale cress)	APT1	NP_564284.1

Gene Ontology
Bibliography

Related articles in PubMed

MHC class II-associated proteins in B-cell exosomes and potential functional implications for exosome biogenesis.
Buschow SI, van Balkom BW, Aalberts M, Heck AJ, Wauben M, Stoorvogel W
Immunology and cell biology88(8)851-6(2010 Nov-Dec)

Large-scale proteomics and phosphoproteomics of urinary exosomes.
Gonzales PA, Pisitkun T, Hoffert JD, Tchapyjnikov D, Star RA, Kleta R, Wang NS, Knepper MA
Journal of the American Society of Nephrology : JASN20(2)363-79(2009 Feb)

Identification of two novel mutations in adenine phosphoribosyltransferase gene in patients with 2,8-dihydroxyadenine urolithiasis.
Taniguchi A, Tsuchida S, Kuno S, Mita M, Machida T, Ioritani N, Terai C, Yamanaka H, Kamatani N
Nucleosides, nucleotides & nucleic acids23(8-9)1141-5(2004 Oct)

Three-dimensional structure of human adenine phosphoribosyltransferase and its relation to DHA-urolithiasis.
Silva M, Silva CH, Iulek J, Thiemann OH
Biochemistry43(24)7663-71(2004 Jun)

A role for certain mouse Aprt sequences in resistance to toxic adenine analogs.
Khattar NH, Turker MS
Somatic cell and molecular genetics23(1)51-61(1997 Jan)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following Aprt gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Aprt cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
ORa03134	NM_001013061.1 Latest version!	Rattus norvegicus adenine phosphoribosyl transferase (Aprt), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$69.30 $99.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
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*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Aprt ( NM_001013061.1 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> NP_001013079.1 Rattus norvegicus adenine phosphoribosyl transferase (Aprt), mRNA.

Price & Availability↑

CloneID	ORa03134
Clone ID Related Accession (Same CDS sequence)	NM_001013061.1
Accession Version	NM_001013061.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 543bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-11-14
Organism	Rattus norvegicus(Norway rat)
Product	adenine phosphoribosyltransferase
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from L04970.1. On Apr 20, 2005 this sequence version replaced XM_214704.2. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: FM085300.1, FQ218973.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMD00052296, SAMD00052297 [ECO:0000348] ##Evidence-Data-END##

ATGTCCGAAT CTGAGTTGCA GCTGGTGGCG CGGCGCATCC GCAGCTTCCC GGACTTCCCC 
ATCCCGGGCG TGCTGTTCAG GGATATCTCG CCCCTCTTGA AAGATCCGGA CTCCTTCCGA 
GCTTCCATCC GCCTCCTGGC CGGTCACCTG AAGTCCACGC ACGGCGGCAA GATCGACTAC 
ATCGCAGGTC TGGACTCCAG GGGCTTCCTG TTTGGCCCTT CCCTTGCTCA GGAGCTGGGG 
GTGGGCTGTG TGCTCATCCG TAAGCGCGGG AAGCTACCAG GCCCCACTGT ATCAGCCTCC 
TATTCTCTAG AGTATGGGAA GGCTGAGCTG GAAATCCAGA AAGATGCCTT AGAACCGGGG 
CAGAAAGTGG TCATTGTGGA TGATCTCCTG GCCACTGGAG GAACCATGTG TGCAGCCTGT 
GAGCTGCTGA GCCAGCTGCG GGCTGAGGTG GTGGAGTGTG TGAGCCTGGT GGAGCTGACC 
TCCCTGAAGG GCAGGGAGAA GCTGGGACCT GTGCCATTCT TCTCTCTCCT GCAGTATGAG 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001013079.1
CDS	1..543
Translation	MSESELQLVARRIRSFPDFPIPGVLFRDISPLLKDPDSFRASIRLLAGHLKSTHGGKIDYIAGLDSRGFLFGPSLAQELGVGCVLIRKRGKLPGPTVSASYSLEYGKAELEIQKDALEPGQKVVIVDDLLATGGTMCAACELLSQLRAEVVECVSLVELTSLKGREKLGPVPFFSLLQYE

Target ORF information:

RefSeq Version	NM_001013061.1
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus adenine phosphoribosyl transferase (Aprt), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001013061.1

ORF Insert Sequence:

ATGTCCGAAT CTGAGTTGCA GCTGGTGGCG CGGCGCATCC GCAGCTTCCC GGACTTCCCC 
ATCCCGGGCG TGCTGTTCAG GGATATCTCG CCCCTCTTGA AAGATCCGGA CTCCTTCCGA 
GCTTCCATCC GCCTCCTGGC CGGTCACCTG AAGTCCACGC ACGGCGGCAA GATCGACTAC 
ATCGCAGGTC TGGACTCCAG GGGCTTCCTG TTTGGCCCTT CCCTTGCTCA GGAGCTGGGG 
GTGGGCTGTG TGCTCATCCG TAAGCGCGGG AAGCTACCAG GCCCCACTGT ATCAGCCTCC 
TATTCTCTAG AGTATGGGAA GGCTGAGCTG GAAATCCAGA AAGATGCCTT AGAACCGGGG 
CAGAAAGTGG TCATTGTGGA TGATCTCCTG GCCACTGGAG GAACCATGTG TGCAGCCTGT 
GAGCTGCTGA GCCAGCTGCG GGCTGAGGTG GTGGAGTGTG TGAGCCTGGT GGAGCTGACC 
TCCCTGAAGG GCAGGGAGAA GCTGGGACCT GTGCCATTCT TCTCTCTCCT GCAGTATGAG 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

MHC class II-associated proteins in B-cell exosomes and potential functional implications for exosome biogenesis.
Immunology and cell biology88(8)851-6(2010 Nov-Dec)
Buschow SI,van Balkom BW,Aalberts M,Heck AJ,Wauben M,Stoorvogel W

Large-scale proteomics and phosphoproteomics of urinary exosomes.
Journal of the American Society of Nephrology : JASN20(2)363-79(2009 Feb)
Gonzales PA,Pisitkun T,Hoffert JD,Tchapyjnikov D,Star RA,Kleta R,Wang NS,Knepper MA

Identification of two novel mutations in adenine phosphoribosyltransferase gene in patients with 2,8-dihydroxyadenine urolithiasis.
Nucleosides, nucleotides & nucleic acids23(8-9)1141-5(2004 Oct)
Taniguchi A,Tsuchida S,Kuno S,Mita M,Machida T,Ioritani N,Terai C,Yamanaka H,Kamatani N

Three-dimensional structure of human adenine phosphoribosyltransferase and its relation to DHA-urolithiasis.
Biochemistry43(24)7663-71(2004 Jun)
Silva M,Silva CH,Iulek J,Thiemann OH

A role for certain mouse Aprt sequences in resistance to toxic adenine analogs.
Somatic cell and molecular genetics23(1)51-61(1997 Jan)
Khattar NH,Turker MS