Nck2 cDNA ORF clone, Mus musculus(house mouse)

Gene
Clones
gRNAs

Summary

Gene Symbol	Nck2
Entrez Gene ID	17974
Full Name	non-catalytic region of tyrosine kinase adaptor protein 2
Synonyms	4833426I10Rik,Grb4,NCKbeta,Nck-2
General protein information	Preferred Names non-catalytic region of tyrosine kinase adaptor protein 2 Names cytoplasmic protein NCK2 NCK adaptor protein 2 SH2/SH3 adaptor protein NCK-beta growth factor receptor-bound protein 4
Gene Type	protein-coding
Organism	Mus musculus(house mouse)
Genome	Chromosome: 1 Map Location: 1\|1 C1.1

mRNA and Protein(s)

mRNA	Protein	Name
XM_030252054.1	XP_030107914.1	cytoplasmic protein NCK2 isoform X1
NM_010879.3	NP_035009.3	cytoplasmic protein NCK2

Pathways from BioSystems

Homologies

Xenopus tropicalis (tropical clawed frog)	nck2	NP_001007985.1
Pan troglodytes (chimpanzee)	NCK2	XP_003309223.1
Canis lupus familiaris (dog)	NCK2	XP_005626042.1
Mus musculus (house mouse)	Nck2	NP_035009.3
Danio rerio (zebrafish)	nck2b	NP_001003492.2
Gallus gallus (chicken)	NCK2	NP_001025985.1
Homo sapiens (human)	NCK2	NP_003572.2
Bos taurus (cattle)	NCK2	NP_001092506.1
Caenorhabditis elegans (roundworm)	nck-1	NP_508706.2
	NCK2	XP_001109452.2
Rattus norvegicus (Norway rat)	Nck2	NP_001101686.1

Gene Ontology
Bibliography

Related articles in PubMed

Nck2, an unexpected regulator of adipogenesis.
Haider N, Dusseault J, Rudich A, Larose L
Adipocyte6(2)154-160(2017 Apr)

Nck2 Deficiency in Mice Results in Increased Adiposity Associated With Adipocyte Hypertrophy and Enhanced Adipogenesis.
Dusseault J, Li B, Haider N, Goyette MA, C?t? JF, Larose L
Diabetes65(9)2652-66(2016 09)

Targeting NCK-Mediated Endothelial Cell Front-Rear Polarity Inhibits Neovascularization.
Dubrac A, Genet G, Ola R, Zhang F, Pibouin-Fragner L, Han J, Zhang J, Thomas JL, Chedotal A, Schwartz MA, Eichmann A
Circulation133(4)409-21(2016 Jan)

Nck influences preosteoblastic/osteoblastic migration and bone mass.
Aryal A C S, Miyai K, Izu Y, Hayata T, Notomi T, Noda M, Ezura Y
Proceedings of the National Academy of Sciences of the United States of America112(50)15432-7(2015 Dec)

The SH2/SH3 adaptor Grb4 transduces B-ephrin reverse signals.
Cowan CA, Henkemeyer M
Nature413(6852)174-9(2001 Sep)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Authors propose that Nck2 should be further investigated as a regulator of the reliance of white adipose tissue on hyperplasia versus hypertrophy during adipose tissue expansion, and hence, as a potential novel molecular target in obesity.
Title: Nck2, an unexpected regulator of adipogenesis.

Findings reveal that Nck2 is a novel regulator of adiposity and suggest that Nck2 is important in limiting WAT expansion and dysfunction in mice and humans.
Title: Nck2 Deficiency in Mice Results in Increased Adiposity Associated With Adipocyte Hypertrophy and Enhanced Adipogenesis.

Inactivation of NCK2 (and NCK1) inhibits endothelial cell polarity.
Title: Targeting NCK-Mediated Endothelial Cell Front-Rear Polarity Inhibits Neovascularization.

These results reveal that Nck regulates preosteoblastic/osteoblastic migration and bone mass.
Title: Nck influences preosteoblastic/osteoblastic migration and bone mass.

we show that the non-catalytic region of tyrosine kinase adaptor (NCK) proteins 1 and 2 are distributed in the developing spinal cord
Title: NCK is critical for the development of deleted in colorectal cancer (DCC) sensitive spinal circuits.

The following Nck2 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Nck2 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OMu22544	NM_010879.3 Latest version!	Mus musculus non-catalytic region of tyrosine kinase adaptor protein 2 (Nck2), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$188.30 $269.00
OMu22544	XM_030252054.1 Latest version!	Mus musculus non-catalytic region of tyrosine kinase adaptor protein 2 (Nck2), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$223.30 $319.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Nck2 ( NM_010879.3 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_035009.3 Mus musculus non-catalytic region of tyrosine kinase adaptor protein 2 (Nck2), mRNA.

Price & Availability↑

CloneID	OMu22544
Clone ID Related Accession (Same CDS sequence)	XM_030252054.1 , NM_010879.3
Accession Version	NM_010879.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1143bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-27
Organism	Mus musculus(house mouse)
Product	cytoplasmic protein NCK2
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AK051665.1, AK014949.1 and BQ176012.1. On Jun 18, 2008 this sequence version replaced NM_010879.2. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AK051665.1, AK014949.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00849374, SAMN00849375 [ECO:0000348] ##Evidence-Data-END##

ATGACAGAAG AAGTCATTGT CATAGCCAAG TGGGACTACA CTGCCCAGCA GGACCAGGAG 
CTCGACATCA GGAAGAACGA GCGGTTGTGG CTGCTAGATG ACTCCAAGAC GTGGTGGCGG 
GTGAGGAATG CTGCCAACAG GACAGGCTAC GTGCCTTCCA ACTATGTGGA GCGTAAGAAC 
AGCCTGAAGA AGGGCTCCCT GGTGAAGAAC CTCAAGGACA CACTAGGCCT CGGAAAGACG 
CGCAGGAAGC CAAGCGCTCG GGATGCTTCC CCAACGCCAA GCACTGATGC GGAGTACCCA 
GCCAATGGCA GCGGTGCTGA CCGCATCTAT GACCTCAACA TCCCAGCCTT TGTCAAGTTC 
GCCTACGTAG CAGAACGAGA GGATGAGCTG TCCCTGGTGA AGGGCTCCCG TGTCACAGTT 
ATGGAGAAAT GCAGTGATGG CTGGTGGCGT GGCAGCTTCA ACGGGCAGAT CGGCTGGTTC 
CCCTCCAACT ACGTTTTGGA GGAAGCGGAC GAAGCTGCGG CTGAGGCCCC CAGCTTCCTG 
AGCCTACGTC GGGGCACTGC ACTGAGCAAT GGGCAGGGTG CACGGGTGCT GCACGTGGTG 
CAGACGCTGT ACCCCTTCAG CTCGGTCACT GAGGAGGAGC TTAGTTTCGA GAAGGGGGAG 
ACCATGGAGG TGATTGAGAA GCCGGAGAAT GACCCTGAAT GGTGGAAATG CAAAAATGCC 
CGAGGCCAAG TGGGCCTGGT CCCCAAAAAC TACGTGGTTG TTCTCAGTGA TGGGCCTGCT 
CTGCACCCCG CTCACACCCC CCAGATCAGC TACACCGGGC CTTCAGCCAG CGGGCGCTTT 
GCTGGTCGGG AGTGGTACTA TGGCAACGTG ACACGGCACC AGGCCGAGTG TGCGCTCAAT 
GAGCGGGGCG TCGAGGGCGA CTTCCTCATT AGGGACAGCG AGTCCTCGCC CAGTGACTTC 
TCCGTGTCTC TCAAAGCGTC AGGGAGAAAC AAGCACTTCA AGGTGCAGCT GGTGGACAGC 
GTCTACTGCA TTGGGCAGCG GCGGTTCCAC AGCATGGACG AGCTTGTGGA GCACTACAAG 
AAGGCCCCCA TCTTCACCAG CGAGCACGGG GAGAAGCTCT ACCTTGTCCG AGCCCTACAG 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_035009.3
CDS	457..1599
Translation	MTEEVIVIAKWDYTAQQDQELDIRKNERLWLLDDSKTWWRVRNAANRTGYVPSNYVERKNSLKKGSLVKNLKDTLGLGKTRRKPSARDASPTPSTDAEYPANGSGADRIYDLNIPAFVKFAYVAEREDELSLVKGSRVTVMEKCSDGWWRGSFNGQIGWFPSNYVLEEADEAAAEAPSFLSLRRGTALSNGQGARVLHVVQTLYPFSSVTEEELSFEKGETMEVIEKPENDPEWWKCKNARGQVGLVPKNYVVVLSDGPALHPAHTPQISYTGPSASGRFAGREWYYGNVTRHQAECALNERGVEGDFLIRDSESSPSDFSVSLKASGRNKHFKVQLVDSVYCIGQRRFHSMDELVEHYKKAPIFTSEHGEKLYLVRALQ

Target ORF information:

RefSeq Version	NM_010879.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus non-catalytic region of tyrosine kinase adaptor protein 2 (Nck2), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_010879.3

ORF Insert Sequence:

ATGACAGAAG AAGTCATTGT CATAGCCAAG TGGGACTACA CTGCCCAGCA GGACCAGGAG 
CTCGACATCA GGAAGAACGA GCGGTTGTGG CTGCTAGATG ACTCCAAGAC GTGGTGGCGG 
GTGAGGAATG CTGCCAACAG GACAGGCTAC GTGCCTTCCA ACTATGTGGA GCGTAAGAAC 
AGCCTGAAGA AGGGCTCCCT GGTGAAGAAC CTCAAGGACA CACTAGGCCT CGGAAAGACG 
CGCAGGAAGC CAAGCGCTCG GGATGCTTCC CCAACGCCAA GCACTGATGC GGAGTACCCA 
GCCAATGGCA GCGGTGCTGA CCGCATCTAT GACCTCAACA TCCCAGCCTT TGTCAAGTTC 
GCCTACGTAG CAGAACGAGA GGATGAGCTG TCCCTGGTGA AGGGCTCCCG TGTCACAGTT 
ATGGAGAAAT GCAGTGATGG CTGGTGGCGT GGCAGCTTCA ACGGGCAGAT CGGCTGGTTC 
CCCTCCAACT ACGTTTTGGA GGAAGCGGAC GAAGCTGCGG CTGAGGCCCC CAGCTTCCTG 
AGCCTACGTC GGGGCACTGC ACTGAGCAAT GGGCAGGGTG CACGGGTGCT GCACGTGGTG 
CAGACGCTGT ACCCCTTCAG CTCGGTCACT GAGGAGGAGC TTAGTTTCGA GAAGGGGGAG 
ACCATGGAGG TGATTGAGAA GCCGGAGAAT GACCCTGAAT GGTGGAAATG CAAAAATGCC 
CGAGGCCAAG TGGGCCTGGT CCCCAAAAAC TACGTGGTTG TTCTCAGTGA TGGGCCTGCT 
CTGCACCCCG CTCACACCCC CCAGATCAGC TACACCGGGC CTTCAGCCAG CGGGCGCTTT 
GCTGGTCGGG AGTGGTACTA TGGCAACGTG ACACGGCACC AGGCCGAGTG TGCGCTCAAT 
GAGCGGGGCG TCGAGGGCGA CTTCCTCATT AGGGACAGCG AGTCCTCGCC CAGTGACTTC 
TCCGTGTCTC TCAAAGCGTC AGGGAGAAAC AAGCACTTCA AGGTGCAGCT GGTGGACAGC 
GTCTACTGCA TTGGGCAGCG GCGGTTCCAC AGCATGGACG AGCTTGTGGA GCACTACAAG 
AAGGCCCCCA TCTTCACCAG CGAGCACGGG GAGAAGCTCT ACCTTGTCCG AGCCCTACAG 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Nck2 ( XM_030252054.1 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_030107914.1 Mus musculus non-catalytic region of tyrosine kinase adaptor protein 2 (Nck2), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OMu22544
Clone ID Related Accession (Same CDS sequence)	XM_030252054.1 , NM_010879.3
Accession Version	XM_030252054.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1143bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	cytoplasmic protein NCK2 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000067.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGACAGAAG AAGTCATTGT CATAGCCAAG TGGGACTACA CTGCCCAGCA GGACCAGGAG 
CTCGACATCA GGAAGAACGA GCGGTTGTGG CTGCTAGATG ACTCCAAGAC GTGGTGGCGG 
GTGAGGAATG CTGCCAACAG GACAGGCTAC GTGCCTTCCA ACTATGTGGA GCGTAAGAAC 
AGCCTGAAGA AGGGCTCCCT GGTGAAGAAC CTCAAGGACA CACTAGGCCT CGGAAAGACG 
CGCAGGAAGC CAAGCGCTCG GGATGCTTCC CCAACGCCAA GCACTGATGC GGAGTACCCA 
GCCAATGGCA GCGGTGCTGA CCGCATCTAT GACCTCAACA TCCCAGCCTT TGTCAAGTTC 
GCCTACGTAG CAGAACGAGA GGATGAGCTG TCCCTGGTGA AGGGCTCCCG TGTCACAGTT 
ATGGAGAAAT GCAGTGATGG CTGGTGGCGT GGCAGCTTCA ACGGGCAGAT CGGCTGGTTC 
CCCTCCAACT ACGTTTTGGA GGAAGCGGAC GAAGCTGCGG CTGAGGCCCC CAGCTTCCTG 
AGCCTACGTC GGGGCACTGC ACTGAGCAAT GGGCAGGGTG CACGGGTGCT GCACGTGGTG 
CAGACGCTGT ACCCCTTCAG CTCGGTCACT GAGGAGGAGC TTAGTTTCGA GAAGGGGGAG 
ACCATGGAGG TGATTGAGAA GCCGGAGAAT GACCCTGAAT GGTGGAAATG CAAAAATGCC 
CGAGGCCAAG TGGGCCTGGT CCCCAAAAAC TACGTGGTTG TTCTCAGTGA TGGGCCTGCT 
CTGCACCCCG CTCACACCCC CCAGATCAGC TACACCGGGC CTTCAGCCAG CGGGCGCTTT 
GCTGGTCGGG AGTGGTACTA TGGCAACGTG ACACGGCACC AGGCCGAGTG TGCGCTCAAT 
GAGCGGGGCG TCGAGGGCGA CTTCCTCATT AGGGACAGCG AGTCCTCGCC CAGTGACTTC 
TCCGTGTCTC TCAAAGCGTC AGGGAGAAAC AAGCACTTCA AGGTGCAGCT GGTGGACAGC 
GTCTACTGCA TTGGGCAGCG GCGGTTCCAC AGCATGGACG AGCTTGTGGA GCACTACAAG 
AAGGCCCCCA TCTTCACCAG CGAGCACGGG GAGAAGCTCT ACCTTGTCCG AGCCCTACAG 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_030107914.1
CDS	358..1500
Translation	MTEEVIVIAKWDYTAQQDQELDIRKNERLWLLDDSKTWWRVRNAANRTGYVPSNYVERKNSLKKGSLVKNLKDTLGLGKTRRKPSARDASPTPSTDAEYPANGSGADRIYDLNIPAFVKFAYVAEREDELSLVKGSRVTVMEKCSDGWWRGSFNGQIGWFPSNYVLEEADEAAAEAPSFLSLRRGTALSNGQGARVLHVVQTLYPFSSVTEEELSFEKGETMEVIEKPENDPEWWKCKNARGQVGLVPKNYVVVLSDGPALHPAHTPQISYTGPSASGRFAGREWYYGNVTRHQAECALNERGVEGDFLIRDSESSPSDFSVSLKASGRNKHFKVQLVDSVYCIGQRRFHSMDELVEHYKKAPIFTSEHGEKLYLVRALQ

Target ORF information:

RefSeq Version	XM_030252054.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus non-catalytic region of tyrosine kinase adaptor protein 2 (Nck2), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_030252054.1

ORF Insert Sequence:

ATGACAGAAG AAGTCATTGT CATAGCCAAG TGGGACTACA CTGCCCAGCA GGACCAGGAG 
CTCGACATCA GGAAGAACGA GCGGTTGTGG CTGCTAGATG ACTCCAAGAC GTGGTGGCGG 
GTGAGGAATG CTGCCAACAG GACAGGCTAC GTGCCTTCCA ACTATGTGGA GCGTAAGAAC 
AGCCTGAAGA AGGGCTCCCT GGTGAAGAAC CTCAAGGACA CACTAGGCCT CGGAAAGACG 
CGCAGGAAGC CAAGCGCTCG GGATGCTTCC CCAACGCCAA GCACTGATGC GGAGTACCCA 
GCCAATGGCA GCGGTGCTGA CCGCATCTAT GACCTCAACA TCCCAGCCTT TGTCAAGTTC 
GCCTACGTAG CAGAACGAGA GGATGAGCTG TCCCTGGTGA AGGGCTCCCG TGTCACAGTT 
ATGGAGAAAT GCAGTGATGG CTGGTGGCGT GGCAGCTTCA ACGGGCAGAT CGGCTGGTTC 
CCCTCCAACT ACGTTTTGGA GGAAGCGGAC GAAGCTGCGG CTGAGGCCCC CAGCTTCCTG 
AGCCTACGTC GGGGCACTGC ACTGAGCAAT GGGCAGGGTG CACGGGTGCT GCACGTGGTG 
CAGACGCTGT ACCCCTTCAG CTCGGTCACT GAGGAGGAGC TTAGTTTCGA GAAGGGGGAG 
ACCATGGAGG TGATTGAGAA GCCGGAGAAT GACCCTGAAT GGTGGAAATG CAAAAATGCC 
CGAGGCCAAG TGGGCCTGGT CCCCAAAAAC TACGTGGTTG TTCTCAGTGA TGGGCCTGCT 
CTGCACCCCG CTCACACCCC CCAGATCAGC TACACCGGGC CTTCAGCCAG CGGGCGCTTT 
GCTGGTCGGG AGTGGTACTA TGGCAACGTG ACACGGCACC AGGCCGAGTG TGCGCTCAAT 
GAGCGGGGCG TCGAGGGCGA CTTCCTCATT AGGGACAGCG AGTCCTCGCC CAGTGACTTC 
TCCGTGTCTC TCAAAGCGTC AGGGAGAAAC AAGCACTTCA AGGTGCAGCT GGTGGACAGC 
GTCTACTGCA TTGGGCAGCG GCGGTTCCAC AGCATGGACG AGCTTGTGGA GCACTACAAG 
AAGGCCCCCA TCTTCACCAG CGAGCACGGG GAGAAGCTCT ACCTTGTCCG AGCCCTACAG 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Nck2, an unexpected regulator of adipogenesis.
Adipocyte6(2)154-160(2017 Apr)
Haider N,Dusseault J,Rudich A,Larose L

Nck2 Deficiency in Mice Results in Increased Adiposity Associated With Adipocyte Hypertrophy and Enhanced Adipogenesis.
Diabetes65(9)2652-66(2016 09)
Dusseault J,Li B,Haider N,Goyette MA,C?t? JF,Larose L

Targeting NCK-Mediated Endothelial Cell Front-Rear Polarity Inhibits Neovascularization.
Circulation133(4)409-21(2016 Jan)
Dubrac A,Genet G,Ola R,Zhang F,Pibouin-Fragner L,Han J,Zhang J,Thomas JL,Chedotal A,Schwartz MA,Eichmann A

Nck influences preosteoblastic/osteoblastic migration and bone mass.
Proceedings of the National Academy of Sciences of the United States of America112(50)15432-7(2015 Dec)
Aryal A C S,Miyai K,Izu Y,Hayata T,Notomi T,Noda M,Ezura Y

The SH2/SH3 adaptor Grb4 transduces B-ephrin reverse signals.
Nature413(6852)174-9(2001 Sep)
Cowan CA,Henkemeyer M