Cyp2a4 cDNA ORF clone, Mus musculus(house mouse)

Gene
Clones
gRNAs

Summary

Gene Symbol	Cyp2a4
Entrez Gene ID	13086
Full Name	cytochrome P450, family 2, subfamily a, polypeptide 4
Synonyms	AI893559,Cyp15a1,D7Ucla4
General protein information	Preferred Names cytochrome P450, family 2, subfamily a, polypeptide 4 Names cytochrome P450 2A4 CYPIIA4 cytochrome P450, 2a4 cytochrome P450-15-alpha cytochrome P450-IIA3.1 testosterone 15-alpha-hydroxylase testosterone 15alpha-hydroxylase
Gene Type	protein-coding
Organism	Mus musculus(house mouse)
Genome	Chromosome: 7 Map Location: 7 A3\|7 14.39 cM

mRNA and Protein(s)

mRNA	Protein	Name
NM_009997.2	NP_034127.2	cytochrome P450 2A4

Pathways from BioSystems

Homologies

Caenorhabditis elegans (roundworm)	cyp-36A1	NP_492267.1
Xenopus tropicalis (tropical clawed frog)	LOC101730237	XP_004917147.1
Homo sapiens (human)	CYP2A13	NP_000757.2
	CYP2A24	NP_001035305.1
Xenopus tropicalis (tropical clawed frog)	cyp2a6	NP_001010998.1
Xenopus tropicalis (tropical clawed frog)	LOC100496861	XP_002939423.1
Homo sapiens (human)	CYP2A6	NP_000753.3
Homo sapiens (human)	CYP2A7	NP_000755.2
Mus musculus (house mouse)	Cyp2a4	NP_034127.2
Rattus norvegicus (Norway rat)	Cyp2a3	NP_036674.1
Xenopus tropicalis (tropical clawed frog)	LOC100170416	NP_001123668.1
Mus musculus (house mouse)	Cyp2a5	NP_031838.2
Xenopus tropicalis (tropical clawed frog)	cyp2f2	NP_001010999.1
Xenopus tropicalis (tropical clawed frog)	LOC548374	XP_002939424.2
Xenopus tropicalis (tropical clawed frog)	LOC100486002	XP_004917148.1

Gene Ontology
Bibliography

Related articles in PubMed

Zinc Fingers and Homeoboxes 2 (Zhx2) Regulates Sexually Dimorphic Cyp Gene Expression in the Adult Mouse Liver.
Creasy KT, Jiang J, Ren H, Peterson ML, Spear BT
Gene expression17(1)7-17(2016)

Impact of nicotine metabolism on nicotine's pharmacological effects and behavioral responses: insights from a Cyp2a(4/5)bgs-null mouse.
Li L, Jia K, Zhou X, McCallum SE, Hough LB, Ding X
The Journal of pharmacology and experimental therapeutics347(3)746-54(2013 Dec)

Resveratrol improves health and survival of mice on a high-calorie diet.
Baur JA, Pearson KJ, Price NL, Jamieson HA, Lerin C, Kalra A, Prabhu VV, Allard JS, Lopez-Lluch G, Lewis K, Pistell PJ, Poosala S, Becker KG, Boss O, Gwinn D, Wang M, Ramaswamy S, Fishbein KW, Spencer RG, Lakatta EG, Le Couteur D, Shaw RJ, Navas P, Puigserver P, Ingram DK, de Cabo R, Sinclair DA
Nature444(7117)337-42(2006 Nov)

Organization and evolution of the Cyp2 gene cluster on mouse chromosome 7, and comparison with the syntenic human cluster.
Wang H, Donley KM, Keeney DS, Hoffman SM
Environmental health perspectives111(15)1835-42(2003 Nov)

Alteration of the substrate specificity of mouse 2A P450s by the identity of residue-209: steroid-binding site and orientation.
Negishi M, Iwasaki M, Juvonen RO, Aida K
The Journal of steroid biochemistry and molecular biology43(8)1031-6(1992 Dec)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

we found that the Cyp2a(4/5)bgs-null mice developed a preference for nicotine in a conditioned place preference test
Title: Impact of nicotine metabolism on nicotine's pharmacological effects and behavioral responses: insights from a Cyp2a(4/5)bgs-null mouse.

The following Cyp2a4 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Cyp2a4 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OMu05322	NM_009997.2 Latest version!	Mus musculus cytochrome P450, family 2, subfamily a, polypeptide 4 (Cyp2a4), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5-7	$223.30 $319.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Cyp2a4 ( NM_009997.2 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_034127.2 Mus musculus cytochrome P450, family 2, subfamily a, polypeptide 4 (Cyp2a4), mRNA.

Price & Availability↑

CloneID	OMu05322
Clone ID Related Accession (Same CDS sequence)	NM_009997.2
Accession Version	NM_009997.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1485bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-03
Organism	Mus musculus(house mouse)
Product	cytochrome P450 2A4
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from BC063778.1. On Oct 19, 2006 this sequence version replaced NM_009997.1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC063778.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00849385, SAMN00849386 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGCTGACCT CAGGACTCCT CCTGGTGGCT GCAGTGGCCT TCCTCAGCGT CCTGGTTTTG 
ATGTCTGTCT GGAAGCAGAG AAAGCTCTCA GGAAAGCTGC CTCCAGGACC CACCCCACTG 
CCCTTCGTTG GGAACTTCCT TCAGCTGAAC ACAGAGCAAA TGTACAACTC TCTCATGAAG 
ATCAGCCAAC GTTATGGTCC TGTATTCACC ATCTATCTGG GATCTCGCCG AATTGTGGTG 
CTGTGCGGAC AGGAGACAGT CAAGGAAGCT CTGGTGGACC AAGCTGAGGA ATTCAGCGGG 
CGGGGCGAGC AGGCTACCTT CGACTGGCTT TTCAAAGGCT ATGGCATAGC CTTCAGCAGC 
GGGGAGCGAG CCAAACAGCT AAGGCGCTTC TCCATCACCA CGCTGCGGGA CTTCGGCGTG 
GGGAAGCGTG GCATCGAGGA GCGCATCCAA GAGGAGGCGG GCTTTCTCAT CGATTCATTT 
CGGAAGACGA ACGGTGCTTT CATTGACCCC ACCTTCTACC TTAGCCGAAC AGTCTCCAAT 
GTCATTAGCT CAATCGTCTT CGGGGACCGC TTTGACTATG AGGACAAAGA GTTCCTGTCA 
CTTCTTAGAA TGATGCTGGG AAGCCTCCAG TTCACTGCTA CCTCCATGGG GCAGGTCTAT 
GAGATGTTTT CTTCTGTGAT GAAACACCTG CCAGGGCCCC AGCAACAGGC CTTTAAGGAG 
CTGCAAGGCC TGGAGGACTT TATAACCAAG AAAGTGGAAC ACAATCAGCG CACGCTGGAT 
CCCAATTCCC CAAGGGACTT CATCGACTCC TTCCTCATCC GAATGCTGGA GGAGAAGAAG 
AACCCCAATA CTGAGTTCTA CATGAAGAAC TTGGTGCTGA CTACACTAAA TCTCTTCTTT 
GCTGGCACAG AGACCGTCAG CACCACCCTG CGCTATGGCT TTCTGTTGCT CATGAAGTAC 
CCAGATATTG AGGCCAAGGT CCATGAGGAG ATTGATCGGG TGATTGGCAG GAACCGGCAG 
CCCAAGTATG AGGACCGAAT GAAGATGCCC TATACGGAGG CTGTAATCCA TGAGATCCAG 
AGATTTGCAG ACCTGATCCC CATGGGCCTG GCTCGAAGGG TCACCAAGGA CACCAAGTTT 
CGAGATTTCC TCCTCCCCAA GGGTACTGAA GTATTTCCTA TGCTGGGCTC TGTGCTGAAA 
GACCCCAAGT TCTTCTCCAA CCCCAAAGAC TTCAACCCAA AGCACTTCCT AGATGACAAG 
GGACAGTTTA AGAAGAGTGA TGCCTTTGTG CCCTTTTCCA TTGGAAAACG GTATTGTTTC 
GGAGAAGGAC TGGCTAGGAT GGAACTCTTC CTCTTCCTCA CAAACATCAT GCAGAACTTC 
CACTTCAAAT CCACACAGGA ACCCCAGGAC ATCGATGTGT CTCCTAGACT CGTGGGCTTT 
GTCACGATCC CACCAACCTA CACTATGAGT TTCTTGTCCC GTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_034127.2
CDS	27..1511
Translation	MLTSGLLLVAAVAFLSVLVLMSVWKQRKLSGKLPPGPTPLPFVGNFLQLNTEQMYNSLMKISQRYGPVFTIYLGSRRIVVLCGQETVKEALVDQAEEFSGRGEQATFDWLFKGYGIAFSSGERAKQLRRFSITTLRDFGVGKRGIEERIQEEAGFLIDSFRKTNGAFIDPTFYLSRTVSNVISSIVFGDRFDYEDKEFLSLLRMMLGSLQFTATSMGQVYEMFSSVMKHLPGPQQQAFKELQGLEDFITKKVEHNQRTLDPNSPRDFIDSFLIRMLEEKKNPNTEFYMKNLVLTTLNLFFAGTETVSTTLRYGFLLLMKYPDIEAKVHEEIDRVIGRNRQPKYEDRMKMPYTEAVIHEIQRFADLIPMGLARRVTKDTKFRDFLLPKGTEVFPMLGSVLKDPKFFSNPKDFNPKHFLDDKGQFKKSDAFVPFSIGKRYCFGEGLARMELFLFLTNIMQNFHFKSTQEPQDIDVSPRLVGFVTIPPTYTMSFLSR

Target ORF information:

RefSeq Version	NM_009997.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus cytochrome P450, family 2, subfamily a, polypeptide 4 (Cyp2a4), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_009997.2

ORF Insert Sequence:

ATGCTGACCT CAGGACTCCT CCTGGTGGCT GCAGTGGCCT TCCTCAGCGT CCTGGTTTTG 
ATGTCTGTCT GGAAGCAGAG AAAGCTCTCA GGAAAGCTGC CTCCAGGACC CACCCCACTG 
CCCTTCGTTG GGAACTTCCT TCAGCTGAAC ACAGAGCAAA TGTACAACTC TCTCATGAAG 
ATCAGCCAAC GTTATGGTCC TGTATTCACC ATCTATCTGG GATCTCGCCG AATTGTGGTG 
CTGTGCGGAC AGGAGACAGT CAAGGAAGCT CTGGTGGACC AAGCTGAGGA ATTCAGCGGG 
CGGGGCGAGC AGGCTACCTT CGACTGGCTT TTCAAAGGCT ATGGCATAGC CTTCAGCAGC 
GGGGAGCGAG CCAAACAGCT AAGGCGCTTC TCCATCACCA CGCTGCGGGA CTTCGGCGTG 
GGGAAGCGTG GCATCGAGGA GCGCATCCAA GAGGAGGCGG GCTTTCTCAT CGATTCATTT 
CGGAAGACGA ACGGTGCTTT CATTGACCCC ACCTTCTACC TTAGCCGAAC AGTCTCCAAT 
GTCATTAGCT CAATCGTCTT CGGGGACCGC TTTGACTATG AGGACAAAGA GTTCCTGTCA 
CTTCTTAGAA TGATGCTGGG AAGCCTCCAG TTCACTGCTA CCTCCATGGG GCAGGTCTAT 
GAGATGTTTT CTTCTGTGAT GAAACACCTG CCAGGGCCCC AGCAACAGGC CTTTAAGGAG 
CTGCAAGGCC TGGAGGACTT TATAACCAAG AAAGTGGAAC ACAATCAGCG CACGCTGGAT 
CCCAATTCCC CAAGGGACTT CATCGACTCC TTCCTCATCC GAATGCTGGA GGAGAAGAAG 
AACCCCAATA CTGAGTTCTA CATGAAGAAC TTGGTGCTGA CTACACTAAA TCTCTTCTTT 
GCTGGCACAG AGACCGTCAG CACCACCCTG CGCTATGGCT TTCTGTTGCT CATGAAGTAC 
CCAGATATTG AGGCCAAGGT CCATGAGGAG ATTGATCGGG TGATTGGCAG GAACCGGCAG 
CCCAAGTATG AGGACCGAAT GAAGATGCCC TATACGGAGG CTGTAATCCA TGAGATCCAG 
AGATTTGCAG ACCTGATCCC CATGGGCCTG GCTCGAAGGG TCACCAAGGA CACCAAGTTT 
CGAGATTTCC TCCTCCCCAA GGGTACTGAA GTATTTCCTA TGCTGGGCTC TGTGCTGAAA 
GACCCCAAGT TCTTCTCCAA CCCCAAAGAC TTCAACCCAA AGCACTTCCT AGATGACAAG 
GGACAGTTTA AGAAGAGTGA TGCCTTTGTG CCCTTTTCCA TTGGAAAACG GTATTGTTTC 
GGAGAAGGAC TGGCTAGGAT GGAACTCTTC CTCTTCCTCA CAAACATCAT GCAGAACTTC 
CACTTCAAAT CCACACAGGA ACCCCAGGAC ATCGATGTGT CTCCTAGACT CGTGGGCTTT 
GTCACGATCC CACCAACCTA CACTATGAGT TTCTTGTCCC GTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Zinc Fingers and Homeoboxes 2 (Zhx2) Regulates Sexually Dimorphic Cyp Gene Expression in the Adult Mouse Liver.
Gene expression17(1)7-17(2016)
Creasy KT,Jiang J,Ren H,Peterson ML,Spear BT

Impact of nicotine metabolism on nicotine's pharmacological effects and behavioral responses: insights from a Cyp2a(4/5)bgs-null mouse.
The Journal of pharmacology and experimental therapeutics347(3)746-54(2013 Dec)
Li L,Jia K,Zhou X,McCallum SE,Hough LB,Ding X

Resveratrol improves health and survival of mice on a high-calorie diet.
Nature444(7117)337-42(2006 Nov)
Baur JA,Pearson KJ,Price NL,Jamieson HA,Lerin C,Kalra A,Prabhu VV,Allard JS,Lopez-Lluch G,Lewis K,Pistell PJ,Poosala S,Becker KG,Boss O,Gwinn D,Wang M,Ramaswamy S,Fishbein KW,Spencer RG,Lakatta EG,Le Couteur D,Shaw RJ,Navas P,Puigserver P,Ingram DK,de Cabo R,Sinclair DA

Organization and evolution of the Cyp2 gene cluster on mouse chromosome 7, and comparison with the syntenic human cluster.
Environmental health perspectives111(15)1835-42(2003 Nov)
Wang H,Donley KM,Keeney DS,Hoffman SM

Alteration of the substrate specificity of mouse 2A P450s by the identity of residue-209: steroid-binding site and orientation.
The Journal of steroid biochemistry and molecular biology43(8)1031-6(1992 Dec)
Negishi M,Iwasaki M,Juvonen RO,Aida K