sprb cDNA ORF clone, Danio rerio(zebrafish)

Gene
Clones
gRNAs

Summary

Gene Symbol	sprb
Entrez Gene ID	795445
Full Name	sepiapterin reductase b
Synonyms	zgc:194446
General protein information	Preferred Names sepiapterin reductase b Names sepiapterin reductase b
Gene Type	protein-coding
Organism	Danio rerio(zebrafish)

mRNA and Protein(s)

mRNA	Protein	Name
NM_001128790.1	NP_001122262.1	sepiapterin reductase b

Pathways from BioSystems

Homologies

Pan troglodytes (chimpanzee)	SPR	XP_525784.2
Mus musculus (house mouse)	Spr	NP_035597.2
Danio rerio (zebrafish)	spra	NP_001019601.2
Canis lupus familiaris (dog)	SPR	XP_540234.2
Gallus gallus (chicken)	SPR	XP_423038.1
Danio rerio (zebrafish)	sprb	NP_001122262.1
Drosophila melanogaster (fruit fly)	Sptr	NP_727265.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP008000	XP_317468.3
	SPR	XP_001103332.1
Rattus norvegicus (Norway rat)	Spr	NP_062054.1
Homo sapiens (human)	SPR	NP_003115.1
Bos taurus (cattle)	SPR	NP_001069471.1
Xenopus tropicalis (tropical clawed frog)	spr	NP_001120067.1

Gene Ontology
Bibliography

Related articles in PubMed

Gene evolution and gene expression after whole genome duplication in fish: the PhyloFish database.
Pasquier J, Cabau C, Nguyen T, Jouanno E, Severac D, Braasch I, Journot L, Pontarotti P, Klopp C, Postlethwait JH, Guiguen Y, Bobe J
BMC genomics17368(2016 05)

RFX transcription factors are essential for hearing in mice.
Elkon R, Milon B, Morrison L, Shah M, Vijayakumar S, Racherla M, Leitch CC, Silipino L, Hadi S, Weiss-Gayet M, Barras E, Schmid CD, Ait-Lounis A, Barnes A, Song Y, Eisenman DJ, Eliyahu E, Frolenkov GI, Strome SE, Durand B, Zaghloul NA, Jones SM, Reith W, Hertzano R
Nature communications68549(2015 Oct)

Pigmentation pathway evolution after whole-genome duplication in fish.
Braasch I, Brunet F, Volff JN, Schartl M
Genome biology and evolution1479-93(2009 Nov)

Evolution of pigment synthesis pathways by gene and genome duplication in fish.
Braasch I, Schartl M, Volff JN
BMC evolutionary biology774(2007 May)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following sprb gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the sprb cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
ODa33409	NM_001128790.1 Latest version!	Danio rerio sepiapterin reductase b (sprb), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$174.30 $249.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
30% OFF

*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

sprb ( NM_001128790.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> NP_001122262.1 Danio rerio sepiapterin reductase b (sprb), mRNA.

Price & Availability↑

CloneID	ODa33409
Clone ID Related Accession (Same CDS sequence)	NM_001128790.1
Accession Version	NM_001128790.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 819bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-11-23
Organism	Danio rerio(zebrafish)
Product	sepiapterin reductase b
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from BC163650.1. On Jul 31, 2008 this sequence version replaced XM_001335582.2. ##Evidence-Data-START## Transcript exon combination :: BC163650.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA3505378, SAMEA3505389 [ECO:0000348] ##Evidence-Data-END##

ATGGACAGCG TGTTGTGCAG CGAGCGTAGG GATCTGGGCC GTGCGGTGTG TGTGGTCACC 
GGAGCGTCGA AAGGCTACGG CCGGACGCTG GCTCTGCAGA TCAGCTGTTT ACTGAAGCCG 
CGCTCCGTCC TGCTGCTGGT GTCCCGAACC GCAGACCAGC TCAACCAGCT GAAGCAGGAC 
ATCGTGAGTC TGCACGCGGC ACACACTGAT CTGCAGCTGG ACGTGCGCTG TGTGCAGGCC 
GATCTGCAGG AGCCCGACGG CGTGCAGAAG ACCATACAGG AGACCAGAAA CACTCTGAGC 
ACTGATATAG AGCATCTGCT GATCATCAAC AACGCAGCAT CTCTGGGTGA CGTGTCCCGC 
TGTGCTGTGA GCTTCACTGA CCCGTGTGAA GTGTCCCGGT ATCTGGCTCT GAATGTGAGC 
GGTGTGTTGA GTCTGACGGC GGGGATCCTG CAGGCGTTTC CTCCAGCGGT CGGCCTGCGC 
AGGACGGTGG TCAACATCAG CTCTCTGTGC GCCCTCAAGC CCTTCCCCTC ATGGGTGCTG 
TACTGCACGG GGAAAGCGGC CCGAGACATG ATGTTCAGGG TGCTGGCGGA GGAGGAGCCC 
GACCTCAGAG TGCTCAGCTA CGCCCCTGGG CCTCTGGACA CTGACATGCA GCTGCAGGCA 
CGCTGTTTTT CAGGTAATGT AGAGCTGCGG CGCTCTTTCT CCTCCATGTT TTCTGACGGT 
CAGCTTCTGT CCTGCGAAGA ATCCGGAGCA AAACTCATGA AGATCCTGCT GGACGACCAA 
TATCCGTCAG GTGCTCATCT GGACTACTAT GAGCTCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001122262.1
CDS	26..844
Translation	MDSVLCSERRDLGRAVCVVTGASKGYGRTLALQISCLLKPRSVLLLVSRTADQLNQLKQDIVSLHAAHTDLQLDVRCVQADLQEPDGVQKTIQETRNTLSTDIEHLLIINNAASLGDVSRCAVSFTDPCEVSRYLALNVSGVLSLTAGILQAFPPAVGLRRTVVNISSLCALKPFPSWVLYCTGKAARDMMFRVLAEEEPDLRVLSYAPGPLDTDMQLQARCFSGNVELRRSFSSMFSDGQLLSCEESGAKLMKILLDDQYPSGAHLDYYEL

Target ORF information:

RefSeq Version	NM_001128790.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio sepiapterin reductase b (sprb), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001128790.1

ORF Insert Sequence:

ATGGACAGCG TGTTGTGCAG CGAGCGTAGG GATCTGGGCC GTGCGGTGTG TGTGGTCACC 
GGAGCGTCGA AAGGCTACGG CCGGACGCTG GCTCTGCAGA TCAGCTGTTT ACTGAAGCCG 
CGCTCCGTCC TGCTGCTGGT GTCCCGAACC GCAGACCAGC TCAACCAGCT GAAGCAGGAC 
ATCGTGAGTC TGCACGCGGC ACACACTGAT CTGCAGCTGG ACGTGCGCTG TGTGCAGGCC 
GATCTGCAGG AGCCCGACGG CGTGCAGAAG ACCATACAGG AGACCAGAAA CACTCTGAGC 
ACTGATATAG AGCATCTGCT GATCATCAAC AACGCAGCAT CTCTGGGTGA CGTGTCCCGC 
TGTGCTGTGA GCTTCACTGA CCCGTGTGAA GTGTCCCGGT ATCTGGCTCT GAATGTGAGC 
GGTGTGTTGA GTCTGACGGC GGGGATCCTG CAGGCGTTTC CTCCAGCGGT CGGCCTGCGC 
AGGACGGTGG TCAACATCAG CTCTCTGTGC GCCCTCAAGC CCTTCCCCTC ATGGGTGCTG 
TACTGCACGG GGAAAGCGGC CCGAGACATG ATGTTCAGGG TGCTGGCGGA GGAGGAGCCC 
GACCTCAGAG TGCTCAGCTA CGCCCCTGGG CCTCTGGACA CTGACATGCA GCTGCAGGCA 
CGCTGTTTTT CAGGTAATGT AGAGCTGCGG CGCTCTTTCT CCTCCATGTT TTCTGACGGT 
CAGCTTCTGT CCTGCGAAGA ATCCGGAGCA AAACTCATGA AGATCCTGCT GGACGACCAA 
TATCCGTCAG GTGCTCATCT GGACTACTAT GAGCTCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Gene evolution and gene expression after whole genome duplication in fish: the PhyloFish database.
BMC genomics17368(2016 05)
Pasquier J,Cabau C,Nguyen T,Jouanno E,Severac D,Braasch I,Journot L,Pontarotti P,Klopp C,Postlethwait JH,Guiguen Y,Bobe J

RFX transcription factors are essential for hearing in mice.
Nature communications68549(2015 Oct)
Elkon R,Milon B,Morrison L,Shah M,Vijayakumar S,Racherla M,Leitch CC,Silipino L,Hadi S,Weiss-Gayet M,Barras E,Schmid CD,Ait-Lounis A,Barnes A,Song Y,Eisenman DJ,Eliyahu E,Frolenkov GI,Strome SE,Durand B,Zaghloul NA,Jones SM,Reith W,Hertzano R

Pigmentation pathway evolution after whole-genome duplication in fish.
Genome biology and evolution1479-93(2009 Nov)
Braasch I,Brunet F,Volff JN,Schartl M

Evolution of pigment synthesis pathways by gene and genome duplication in fish.
BMC evolutionary biology774(2007 May)
Braasch I,Schartl M,Volff JN