GPT cDNA ORF clone, Bison bison bison

Gene
Clones
gRNAs

Summary

Gene Symbol	GPT
Entrez Gene ID	104986881
Full Name	glutamic--pyruvic transaminase
Gene Type	protein-coding
Organism	Bison bison bison

mRNA and Protein(s)

mRNA	Protein	Name
XM_010837579.1	XP_010835881.1	LOW QUALITY PROTEIN: alanine aminotransferase 1

Pathways from BioSystems
Gene Ontology

The following GPT gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the GPT cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OBi62980	XM_010837579.1 Latest version!	Bison bison bison glutamic-pyruvate transaminase (alanine aminotransferase) (GPT), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$258.30 $369.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

GPT ( XM_010837579.1 ) cDNA ORF clone, Bison bison bison -> XP_010835881.1 Bison bison bison glutamic-pyruvate transaminase (alanine aminotransferase) (GPT), mRNA.

Price & Availability↑

CloneID	OBi62980
Clone ID Related Accession (Same CDS sequence)	XM_010837579.1
Accession Version	XM_010837579.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1185bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2014-12-30
Organism	Bison bison bison
Product	LOW QUALITY PROTEIN: alanine aminotransferase 1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_011494650.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bison bison bison Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## ##RefSeq-Attributes-START## frameshifts :: corrected 1 indel ##RefSeq-Attributes-END##

ATGGCCTTGA GGGCAGGTGA GCACAGCCAG GAGGCAGCAA ATGGGCTGAA GGAGAAGGTG 
CTGACACTGG ACTCCATGAA TCCTTATGTC CGGAGGGTGG AGTATGCGGT GCGAGGCCCC 
ATCGTGCAGC GGGCACTGGA GCTGGAGCAG GAGCTGCGCC AGGGCGTAAA GAAGCCCTTC 
ACCGAGGTCA TCCGCGCCAA CATCGGGGAC GCACAGGCCA TGGGGCAGAT CCCTATCACC 
TTCCCGCGCC AGGTCCTGGC CCTCTGCGTC CATCCCGATC TCCTGAACAG CCCTGACTTC 
CCCGACGACG CCAAGAGGAG GGCGGAGCGC ATCTTGCAGG CGTGTGGGGG CCACAGCCTG 
GGGGCCTACA GCATCAGCGC TGGCGTCCAG ATGATCCGTG AGGATGTGGC GCGGTACATC 
GAGCGGCGCG ATGGAGGCAT TCCTGCCGAC CCCAATAACA TCTTTCTGTC CACGGGGGCC 
AGCGATGCCA TTGTGACGGT GCTGAAGTTG CTGGTAACCG GCGAGGACAA CGTGTATGCC 
GAGAGCTCGC AGTTCCACTC GTTCAAGAAG GTGCTCACGG AGATGGGGCC GCCGTACGCG 
GCGCAACAGG AGCTCGCCTC CTTCCACTCG ATCTCCAAGG GCTACATGGG CGAGTGCGGC 
TTCCGCGGCG GCTACGTGGA GGTGGTGAAT ATGGACGCTG CAGTGAAGCA GCAGATGCAG 
AAGCTGCGGA GCGTGCGGCT GTGCCCACCC ACCCCGGGCC AGGTCCTGCC CGATNCTGTC 
AGCCCGCCGG CGCCCTCAGA CCCCTCCTTC CCGCGGTTCC AGGCGGAGAG GCGGGCGGTG 
CTGGCCGAGC TGGCTGCCAA GGCCAAGCTC ACGGAGCAGG TCTTCAACGA AGCTCCCGGC 
ATCCGCTGCA ATCCGGTGCA GGGCGCCATG TATTCGTTCC CGCGCGTGCA GCTGCCCCCG 
CGTGCGGTGC AGCGCGCTCA GGAGCTGGGC CTGGCTCCCG ACATGTTCTT CTGCCTGCGC 
CTCCTAGAGG AGACTGGCAT CTGCGTGGTG CCTGGGAGTG GCTTTGGACA ACGGGAAGGC 
ACCTACCACT TTCGGATGAC TATTCTGCCC CCCATGGAGA AGCTACGGCC CCTGCTGGAG 
AAGCTGAGCC AGTTCCATGC GAAGTTCACC CGCGAGTACT CCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_010835881.1
CDS	856..2040
Translation	MALRAGEHSQEAANGLKEKVLTLDSMNPYVRRVEYAVRGPIVQRALELEQELRQGVKKPFTEVIRANIGDAQAMGQIPITFPRQVLALCVHPDLLNSPDFPDDAKRRAERILQACGGHSLGAYSISAGVQMIREDVARYIERRDGGIPADPNNIFLSTGASDAIVTVLKLLVTGEDNVYAESSQFHSFKKVLTEMGPPYAAQQELASFHSISKGYMGECGFRGGYVEVVNMDAAVKQQMQKLRSVRLCPPTPGQVLPDnullVSPPAPSDPSFPRFQAERRAVLAELAAKAKLTEQVFNEAPGIRCNPVQGAMYSFPRVQLPPRAVQRAQELGLAPDMFFCLRLLEETGICVVPGSGFGQREGTYHFRMTILPPMEKLRPLLEKLSQFHAKFTREYS

Target ORF information:

RefSeq Version	XM_010837579.1
Organism	Bison bison bison
Definition	Bison bison bison glutamic-pyruvate transaminase (alanine aminotransferase) (GPT), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_010837579.1

ORF Insert Sequence:

ATGGCCTTGA GGGCAGGTGA GCACAGCCAG GAGGCAGCAA ATGGGCTGAA GGAGAAGGTG 
CTGACACTGG ACTCCATGAA TCCTTATGTC CGGAGGGTGG AGTATGCGGT GCGAGGCCCC 
ATCGTGCAGC GGGCACTGGA GCTGGAGCAG GAGCTGCGCC AGGGCGTAAA GAAGCCCTTC 
ACCGAGGTCA TCCGCGCCAA CATCGGGGAC GCACAGGCCA TGGGGCAGAT CCCTATCACC 
TTCCCGCGCC AGGTCCTGGC CCTCTGCGTC CATCCCGATC TCCTGAACAG CCCTGACTTC 
CCCGACGACG CCAAGAGGAG GGCGGAGCGC ATCTTGCAGG CGTGTGGGGG CCACAGCCTG 
GGGGCCTACA GCATCAGCGC TGGCGTCCAG ATGATCCGTG AGGATGTGGC GCGGTACATC 
GAGCGGCGCG ATGGAGGCAT TCCTGCCGAC CCCAATAACA TCTTTCTGTC CACGGGGGCC 
AGCGATGCCA TTGTGACGGT GCTGAAGTTG CTGGTAACCG GCGAGGACAA CGTGTATGCC 
GAGAGCTCGC AGTTCCACTC GTTCAAGAAG GTGCTCACGG AGATGGGGCC GCCGTACGCG 
GCGCAACAGG AGCTCGCCTC CTTCCACTCG ATCTCCAAGG GCTACATGGG CGAGTGCGGC 
TTCCGCGGCG GCTACGTGGA GGTGGTGAAT ATGGACGCTG CAGTGAAGCA GCAGATGCAG 
AAGCTGCGGA GCGTGCGGCT GTGCCCACCC ACCCCGGGCC AGGTCCTGCC CGATNCTGTC 
AGCCCGCCGG CGCCCTCAGA CCCCTCCTTC CCGCGGTTCC AGGCGGAGAG GCGGGCGGTG 
CTGGCCGAGC TGGCTGCCAA GGCCAAGCTC ACGGAGCAGG TCTTCAACGA AGCTCCCGGC 
ATCCGCTGCA ATCCGGTGCA GGGCGCCATG TATTCGTTCC CGCGCGTGCA GCTGCCCCCG 
CGTGCGGTGC AGCGCGCTCA GGAGCTGGGC CTGGCTCCCG ACATGTTCTT CTGCCTGCGC 
CTCCTAGAGG AGACTGGCAT CTGCGTGGTG CCTGGGAGTG GCTTTGGACA ACGGGAAGGC 
ACCTACCACT TTCGGATGAC TATTCTGCCC CCCATGGAGA AGCTACGGCC CCTGCTGGAG 
AAGCTGAGCC AGTTCCATGC GAAGTTCACC CGCGAGTACT CCTGA