Protein Analysis Instruments

eStain™ L1 Protein Staining System

10分の簡単操作で高精度なゲル中のタンパク質染色が可能です。

快速： 染色と脱色を10分以内に完了可能！
全自動：試薬の注入および排出は自動的に行われます。
高感度：各タンパク質は12.5ngからシャープな染色バンドとして検出可能！
効率的： ミニゲル2枚分を同時に操作可能！
高信頼性： 均等な染色と再現性の高い結果を取得可能！

eStainL1 は、Genscript によって開発されたPAGEゲル用の高速染色システムです。特許取得済みのタンパク質染色技術を用い、ミニPAGE ゲルのCBC染色と脱色を行うことができます。従来の固定、染色、脱色の3 つの工程を1 つに統合することにより、染色工程は迅速かつ効率的であり、再現性と信頼性が高い結果を得られます。

全工程は10 分以内に完了可能です。染色バッファーと脱色バッファーは、eStainL1 タンパク質染色キットに含まれています。

eStain videos
Introductory video Detailed set up and operation video

eStain™ L1 Resources
eStain User Manual estain L1 Quick Reference Card eStain Flyer

eStainについて

Shortened workflow increases your productivity

The eStain Protein Staining System combines three steps of traditional Coomassie blue staining method into one single step to greatly reduce the time required for protein staining (Table 1).

Table 1: eStain saves hours to overnight compared to conventional staining methods

	wash or Rinse Step	Stain Step	Destain or Rinse Step	Time saved with eStain L1
eStain	0 min	Electric Staining <10 min	0 min
SimplyBlue SafeSatin (Basic Protocol)	Rinse 15 min	Stain 1-3 hr	Wash 1-3 hr	2 hr , 15 min-6 hr, 15 min
Bio-Safe Coomassie Stain	Wash 15 min	Stain 1 hr	Rinse 30 min	1 hr , 45 min
Gelcode Blue Safe Protein Stain (Basic Protocol)	Wash 15 min	Stain 15 min-1 hr	Destain 1-2 hr	1 hr , 30 min - 3 hr , 15 min

Superior sensitivity (≥12.5ng protein)

Gel: ExpressPlus™ PAGE Gel, 4-12%, 10 wells (GenScript M41210)
Program: Stain
Time: 9 min 30 s

Excellent uniformity and repeatability

Gel: ExpressPlus™ PAGE Gel, 4-12%, 10 wells (GenScript, M41210)
Samples: PAGE-MASTER Protein Standard Plus, 5 ul (GenScript, MM1397-500)
Program: Stain
Time: 9 min 30 s

マニュアル

eStain Protein Staining device quick guide

Note: For complete instructions and advanced programming, please refer to the manual

Operational Instructions
Step 1. Add distilled water into the tray provided. When gel electrophoresis is complete, carefully remove the gel from the gel cassette and briefly rinse the gel with distilled water for 1 min
Step 2. Open the gel holder and place it on the table with the fabric side down. Carefully place the rinsed gel on the fabric side of the gel holder. Ensure that the upper part of the gel (with comb) point to the axis of the gel holder and is as close to the axis as possible
Step 3. Pre-wet a piece of filter paper (provided) with distilled water and place it on top of the gel
Step 4. Close the gel holder
Step 5. Choose a Channel and insert the gel holder into the channel. *Note：Please ensure that the fabric side of the gel holder is facing the user before inserting it into the Channel*
Step 6. Press the corresponding channel to start the program. The channel timer starts to countdown
Step 7. The instrument beeps as the program countdown to 0
Step 8. The channel stops flashing and the screen returns to normal. Take out the gel holder and the stained gel. The staining process is complete.