Anti-c-Myc Tag Antibody (9E10), mAb, Mouse
Anti-c-Myc Tag Antibody (9E10), MAb, Mouse

Figure 1: Western blot analysis of c-Myc tagged protein expressed in mammalian cell using Anti-c-Myc Tag Antibody (9E10), mAb, Mouse (GenScript, A02300, 1:1000).
M: Protein Marker
1; c-Myc tagged fusion protein 20 ng
2: c-Myc tagged fusion protein 10 ng
3: c-Myc tagged fusion protein 5 ng
The signal was developed with HRP Conjugated Goat Anti-Mouse IgG.

Anti-c-Myc Tag Antibody (9E10), MAb, Mouse

Figure 2: Flow cytometric analysis of c-Myc tagged fusion proteins expressed in mammalian cell by Anti-c-Myc Tag Antibody (9E10), mAb, Mouse (GenScript, A02300, 1:100) or normal mouse IgG antibody as negative control (black and blue, respectively). The signal was developed with FITC conjugated Goat Anti-Mouse IgG.

Anti-c-Myc Tag Antibody (9E10), mAb, Mouse

Anti-c-Myc Tag Antibody (9E10), mAb, Mouse is specific for Myc tagged proteins.
A02300
¥8,883.00

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Overview
Specificity Anti-c-Myc Tag Antibody (9E10), mAb, Mouse is specific for Myc tagged proteins.
Host Species Mouse

Applications
Working concentrations for specific applications should be determined by the investigator. The appropriate concentrations may be affected by antigen concentration, the sensitivity of the method of detection, temperature, the length of the incubations, and other factors. The suitability of this antibody for applications other than those listed below has not been determined. The following concentration ranges are recommended starting points for this product.
Application Recommended Usage
Western Blot 1:500-1:2,000
Immunohistochemistry (IHC) 1:50-1:1,000
Immunocytochemistry/Immunofluorescence (ICC/IF) 1:100-1:500
Flow Cytometry 1:50-1:200
Immunoprecipitation (IP) 1:100-1:500
ELISA 1:1000-1:10000

Properties
Form Liquid
Storage Buffer Supplied in PBS (pH 7.2), containing 0.03% Proclin 300 and 50% glycerol
Concentration 1.0 mg/ml
Storage Instructions The product remains stable for 1 year at -20℃ from the date of receipt. Avoid repeated freezing and thawing cycles.
Purification Protein A affinity column
Isotype Mouse IgG1
Clonality Monoclonal
Clone ID 9E10
Note GenScript can customize this product per customer's request including product size, buffer components, etc.

Examples
  • Anti-c-Myc Tag Antibody (9E10), MAb, Mouse
    • Anti-c-Myc Tag Antibody (9E10), MAb, Mouse

    Figure 1: Western blot analysis of c-Myc tagged protein expressed in mammalian cell using Anti-c-Myc Tag Antibody (9E10), mAb, Mouse (GenScript, A02300, 1:1000).
    M: Protein Marker
    1; c-Myc tagged fusion protein 20 ng
    2: c-Myc tagged fusion protein 10 ng
    3: c-Myc tagged fusion protein 5 ng
    The signal was developed with HRP Conjugated Goat Anti-Mouse IgG.

  • Anti-c-Myc Tag Antibody (9E10), MAb, Mouse
    • Anti-c-Myc Tag Antibody (9E10), MAb, Mouse

    Figure 2: Flow cytometric analysis of c-Myc tagged fusion proteins expressed in mammalian cell by Anti-c-Myc Tag Antibody (9E10), mAb, Mouse (GenScript, A02300, 1:100) or normal mouse IgG antibody as negative control (black and blue, respectively). The signal was developed with FITC conjugated Goat Anti-Mouse IgG.


Background
Target Background The c-Myc tag is a small peptide tag with amino acid sequence of EQKLISEEDL. It is derived from the c-myc gene product that can be added to a protein using recombinant DNA technology. c-Myc Tag Antibody (9E10) is a famous mouse monoclonal clone for the analysis of c-Myc tagged recombinant protein. The 9E10 clone has been cited in more than 9000 publications, widely used in various applications, such as Western Blot (WB), Flow cytometry (FACS), Immunoprecipitation (IP), Immunofluorescence (IF) and Immunohistochemistry (IHC) and so on. GenScript Anti-c-Myc Tag Antibody (9E10), mAb, Mouse (Cat.No.: A02300) is a recombinant antibody, which has several advantages including: good batch-to-batch consistency, high reproducibility, comparative sensitivity and specificity, and animal-free production
Synonyms c-myc Antibody; c-myc tag antibody, Myc Epitope Tag antibody

For research use only. Not intended for human and animal therapeutic or diagnostic use.