Western blot of IP samples with A01827 or conventional secondary antibodies.
Loading:
Lane 1,3: Protein A beads (40 µl) + Glutamine Synthetase Antibody, pAb, Rabbit (10 µg) + Mouse brain lysate (200 µg)
Lane 2,4: Mouse brain lysate (20 µg)
Primary antibody:
Lane 1~4: Glutamine Synthetase Antibody, pAb, Rabbit
Secondary antibody:
Lane 1,2: Mouse Anti-Rabbit IgG Antibody (M205) [HRP], mAb (GenScript, A01827), 1:5,000
Lane 3,4: Anti-RABBIT IgG (H&L) (GOAT) Antibody Peroxidase Conjugated (Min X Bv Ch Gt GP Ham Hs Hu Ms Rt & Sh Serum Proteins), 1:5,000
Western blot of reduced and non-reduced rabbit IgG with A01827.
Loading:
Lane 1: Rabbit IgG (Reduced), 200 ng
Lane 2: Rabbit IgG (Non-reduced), 200 ng
We recommend loading no more than 200 ng of purified sample per lane
Detection antibody:
Lane 1~2: Mouse Anti-Rabbit IgG Antibody (M205) [HRP], mAb (GenScript, A01827), 1:5,000
Mouse Anti-Rabbit IgG Antibody (M205) [HRP], mAb
A01827 | |
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¥15,881.00 | |
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